Enzyme
stringlengths 4
59
| Organism
stringlengths 1
70
| Substrate
stringlengths 1
86
| km
float64 0
1.4M
⌀ | Unit_Km
stringclasses 65
values | Kcat
stringlengths 1
16
| Unit_Kcat
stringclasses 134
values | Kcat/Km
stringlengths 1
21
| Unit_Kcat/Km
stringclasses 335
values | Commentary[Temp]
stringclasses 165
values | Commentary[pH]
stringclasses 225
values | Commentary[Mutant]
stringlengths 1
93
| Commentary[Cosubstrate]
stringlengths 1
89
| pubmed id
float64 1.5M
35.5M
⌀ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
LysJ | Thermus thermophilus HB27 | N^2-Acetyllysine | 10 | mM | 0.8 | s^-1 | 78.6 | s^-1M^-1 | 45°C | 8.9 | 2-oxoglutarate | 11,489,859 |
|
LysJ | Thermus thermophilus HB27 | N^2-Acetylornithine | 0.8 | mM | 1.0 | s^-1 | 1266.1 | s^-1M^-1 | 45°C | 8.9 | 2-oxoglutarate | 11,489,859 |
|
3α-HSD3 | Homo sapiens | DHT | 1.1 | µM | 1.5 | min^-1 | 1.3 | µM^-1min^-1 | NADPH | 11,514,561 |
|||
3α-HSD3 | Homo sapiens | Testosterone | 0.67 | µM | 0.0071 | min^-1 | 0.011 | µM^-1min^-1 | NADP^+ | 11,514,561 |
|||
Phosphoglucose isomerase | Pyrococcus furiosus | Fructose 6-phosphate | 0.63 | mM | 50°C | 7.0 | Native | 11,533,028 |
|||||
Phosphoglucose isomerase | Pyrococcus furiosus | Glucose 6-phosphate | 1.99 | mM | 50°C | 7.0 | Native | 11,533,028 |
|||||
Phosphoglucose isomerase | Pyrococcus furiosus | Fructose 6-phosphate | 0.42 | mM | 50°C | 7.0 | Recombinant | 11,533,028 |
|||||
Phosphoglucose isomerase | Pyrococcus furiosus | Glucose 6-phosphate | 2 | mM | 50°C | 7.0 | Recombinant | 11,533,028 |
|||||
Phosphoglucose isomerase | Pyrococcus furiosus | Fructose 6-phosphate | null | 11.5 | s^-1mM^-1 | 50°C | 7.0 | Native | 11,533,028 |
||||
Phosphoglucose isomerase | Pyrococcus furiosus | Glucose 6-phosphate | null | 6.2 | s^-1mM^-1 | 50°C | 7.0 | Native | 11,533,028 |
||||
Phosphoglucose isomerase | Pyrococcus furiosus | Fructose 6-phosphate | null | 16.5 | s^-1mM^-1 | 50°C | 7.0 | Recombinant | 11,533,028 |
||||
Phosphoglucose isomerase | Pyrococcus furiosus | Glucose 6-phosphate | null | 8.6 | s^-1mM^-1 | 50°C | 7.0 | Recombinant | 11,533,028 |
||||
UPPs | Escherichia coli | IPP | 4.1 | µM | 2.5 | s^-1 | 25°C | 7.5 | Wild type | FPP (10 µM) | 11,581,264 |
||
UPPs | Escherichia coli | FPP | 0.4 | µM | 2.5 | s^-1 | 25°C | 7.5 | Wild type | IPP (20 µM) | 11,581,264 |
||
UPPs | Escherichia coli | IPP | 133 | µM | 0.11 | s^-1 | 25°C | 7.5 | S71A | FPP (10 µM) | 11,581,264 |
||
UPPs | Escherichia coli | FPP | 1 | µM | 0.11 | s^-1 | 25°C | 7.5 | S71A | IPP (20 µM) | 11,581,264 |
||
UPPs | Escherichia coli | IPP | 8 | µM | 0.022 | s^-1 | 25°C | 7.5 | N74A | FPP (10 µM) | 11,581,264 |
||
UPPs | Escherichia coli | FPP | 0.4 | µM | 0.022 | s^-1 | 25°C | 7.5 | N74A | IPP (20 µM) | 11,581,264 |
||
UPPs | Escherichia coli | IPP | 46 | µM | 1.1 | s^-1 | 25°C | 7.5 | W75A | FPP (10 µM) | 11,581,264 |
||
UPPs | Escherichia coli | FPP | 3.2 | µM | 1.1 | s^-1 | 25°C | 7.5 | W75A | IPP (20 µM) | 11,581,264 |
||
UPPs | Escherichia coli | IPP | 15.7 | µM | 1.4 × 10^-4 | s^-1 | 25°C | 7.5 | R77A | FPP (10 µM) | 11,581,264 |
||
UPPs | Escherichia coli | FPP | 1.6 | µM | 1.4 × 10^-4 | s^-1 | 25°C | 7.5 | R77A | IPP (20 µM) | 11,581,264 |
||
UPPs | Escherichia coli | IPP | 91 | µM | 0.4 | s^-1 | 25°C | 7.5 | E81A | FPP (10 µM) | 11,581,264 |
||
KasA | Mycobacterium tuberculosis | C16:0-ACPEc | 3.2 | µM | 5.3 | min^-1 | 1.7 × 10^6 | M^-1min^-1 | 37°C | 6.8 | malonyl-ACPEc | 11,600,501 |
|
KasA | Mycobacterium tuberculosis | C20:0-ACPEc | 2.5 | µM | 4.5 | min^-1 | 1.8 × 10^6 | M^-1min^-1 | 37°C | 6.8 | malonyl-ACPEc | 11,600,501 |
|
KasA | Mycobacterium tuberculosis | malonyl-ACPEc | 13.5 | µM | 4.8 | min^-1 | 3.5 × 10^5 | M^-1min^-1 | 37°C | 6.8 | C16:0-ACPEc | 11,600,501 |
|
KasB | Mycobacterium tuberculosis | C16:0-ACPEc | 1.4 | µM | 1.6 | min^-1 | 1.1 × 10^6 | M^-1min^-1 | 37°C | 7.0 | malonyl-ACPEc | 11,600,501 |
|
KasB | Mycobacterium tuberculosis | C20:0-ACPEc | 1.9 | µM | 0.6 | min^-1 | 3.2 × 10^5 | M^-1min^-1 | 37°C | 7.0 | malonyl-ACPEc | 11,600,501 |
|
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | Pyruvate | 0.28 | mM | 51 | s^-1 | 182 | s^-1mM^-1 | 80°C | 8.5 | Natural | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | 2-oxoglutarate | 0.85 | mM | 19 | s^-1 | 22 | s^-1mM^-1 | 80°C | 8.5 | Natural | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | Pyruvate | 0.33 | mM | 48 | s^-1 | 146 | s^-1mM^-1 | 80°C | 8.5 | Recombinant | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | 2-oxoglutarate | 0.72 | mM | 17 | s^-1 | 23 | s^-1mM^-1 | 80°C | 8.5 | Recombinant | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | Pyruvate | 0.31 | mM | 2.2 | s^-1 | 7.2 | s^-1mM^-1 | 80°C | 8.5 | Y253F | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | 2-oxoglutarate | 1.5 | mM | 5.3 | s^-1 | 3.6 | s^-1mM^-1 | 80°C | 8.5 | Y253F | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | Pyruvate | 0.17 | mM | 3.6 | s^-1 | 21 | s^-1mM^-1 | 80°C | 8.5 | P254G | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | 2-oxoglutarate | 1.2 | mM | 5.2 | s^-1 | 4.3 | s^-1mM^-1 | 80°C | 8.5 | P254G | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | Pyruvate | 0.28 | mM | 5.7 | s^-1 | 20 | s^-1mM^-1 | 80°C | 8.5 | I255L | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | 2-oxoglutarate | 1.1 | mM | 5.1 | s^-1 | 4.6 | s^-1mM^-1 | 80°C | 8.5 | I255L | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | Pyruvate | 0.13 | mM | 3.2 | s^-1 | 25 | s^-1mM^-1 | 80°C | 8.5 | I255S | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | 2-oxoglutarate | 0.89 | mM | 7.4 | s^-1 | 8.3 | s^-1mM^-1 | 80°C | 8.5 | I255S | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | Pyruvate | 0.19 | mM | 7.9 | s^-1 | 41 | s^-1mM^-1 | 80°C | 8.5 | I255M | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | 2-oxoglutarate | 1.3 | mM | 10 | s^-1 | 7.7 | s^-1mM^-1 | 80°C | 8.5 | I255M | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | Pyruvate | 0.11 | mM | 4.1 | s^-1 | 38 | s^-1mM^-1 | 80°C | 8.5 | T256S | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | 2-oxoglutarate | 2.1 | mM | 7.4 | s^-1 | 3.4 | s^-1mM^-1 | 80°C | 8.5 | T256S | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | Pyruvate | 0.14 | mM | 4.3 | s^-1 | 31 | s^-1mM^-1 | 80°C | 8.5 | T256A | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | 2-oxoglutarate | 0.37 | mM | 5.5 | s^-1 | 15 | s^-1mM^-1 | 80°C | 8.5 | T256A | CoA | 11,683,888 |
2-oxoacid:ferredoxin oxidoreductase | Sulfolobus sp. strain 7 | Pyruvate | 0.17 | mM | 4.6 | s^-1 | 28 | s^-1mM^-1 | 80°C | 8.5 | T256V | CoA | 11,683,888 |
AGX1 | Homo sapiens | UTP | 53 | µM | 1.2 | µM^-1s^-1 | 30°C | 8.0 | Wild-type | 11,707,391 |
|||
AGX1 | Homo sapiens | GlcNAc1P | 5.3 | µM | 12.2 | µM^-1s^-1 | 30°C | 8.0 | Wild-type | 11,707,391 |
|||
AGX1 | Homo sapiens | UDPGlcNAc | 32 | µM | 2.0 | µM^-1s^-1 | 30°C | 8.0 | Wild-type | 11,707,391 |
|||
AGX1 | Homo sapiens | UDPGalNAc | 500 | µM | 0.18 | µM^-1s^-1 | 30°C | 8.0 | Wild-type | 11,707,391 |
|||
AGX2 | Homo sapiens | UTP | 49 | µM | 1.4 | µM^-1s^-1 | 30°C | 8.0 | Wild-type | 11,707,391 |
|||
AGX2 | Homo sapiens | GlcNAc1P | 6 | µM | 11.2 | µM^-1s^-1 | 30°C | 8.0 | Wild-type | 11,707,391 |
|||
AGX2 | Homo sapiens | UDPGlcNAc | 32 | µM | 2.2 | µM^-1s^-1 | 30°C | 8.0 | Wild-type | 11,707,391 |
|||
AGX2 | Homo sapiens | UDPGalNAc | 530 | µM | 0.2 | µM^-1s^-1 | 30°C | 8.0 | Wild-type | 11,707,391 |
|||
AGX1 | Homo sapiens | GlcNAc1P | null | 30°C | 8.0 | Wild-type | 1 mM UTP | 11,707,391 |
|||||
AGX1 | Homo sapiens | GlcNAc1P | null | 30°C | 8.0 | R115A | 1 mM UTP | 11,707,391 |
|||||
AGX2 | Homo sapiens | GlcNAc1P | null | 30°C | 8.0 | Wild-type | 1 mM UTP | 11,707,391 |
|||||
Afung | Archaeoglobus fulgidus | [3H]uracil-containing DNA | 1.34 | µM | 1.05 | min^-1 | 0.782 | µM^-1min^-1 | 70°C | 7.5 | 11,738,943 |
||
Afung | Archaeoglobus fulgidus | [3H]uracil-containing DNA | 1.51 | µM | 1.19 | min^-1 | 0.786 | µM^-1min^-1 | 80°C | 7.5 | 11,738,943 |
||
Afung | Archaeoglobus fulgidus | [3H]uracil-containing DNA | 0.536 | µM | 0.498 | min^-1 | 0.929 | µM^-1min^-1 | 95°C | 7.5 | 11,738,943 |
||
UPPS | Escherichia coli | FPP | 0.4 | µM | 2.5 | s^-1 | 25°C | 7.5 | Wild type | IPP | 11,744,728 |
||
UPPS | Escherichia coli | IPP | 4.1 | µM | 2.5 | s^-1 | 25°C | 7.5 | Wild type | FPP | 11,744,728 |
||
UPPS | Escherichia coli | FPP | 2 | µM | 1.1 | s^-1 | 25°C | 7.5 | W31F | IPP | 11,744,728 |
||
UPPS | Escherichia coli | IPP | 5.9 | µM | 1.1 | s^-1 | 25°C | 7.5 | W31F | FPP | 11,744,728 |
||
UPPS | Escherichia coli | FPP | 1.8 | µM | 1.1 | s^-1 | 25°C | 7.5 | W75F | IPP | 11,744,728 |
||
UPPS | Escherichia coli | IPP | 26 | µM | 1.1 | s^-1 | 25°C | 7.5 | W75F | FPP | 11,744,728 |
||
UPPS | Escherichia coli | FPP | 0.4 | µM | 2.5 | s^-1 | 25°C | 7.5 | W91F | IPP | 11,744,728 |
||
UPPS | Escherichia coli | IPP | 7.2 | µM | 2.5 | s^-1 | 25°C | 7.5 | W91F | FPP | 11,744,728 |
||
UPPS | Escherichia coli | FPP | 0.7 | µM | 1.7 | s^-1 | 25°C | 7.5 | W149F | IPP | 11,744,728 |
||
UPPS | Escherichia coli | IPP | 20 | µM | 1.7 | s^-1 | 25°C | 7.5 | W149F | FPP | 11,744,728 |
||
UPPS | Escherichia coli | FPP | 3.4 | µM | 1.5 | s^-1 | 25°C | 7.5 | W207F | IPP | 11,744,728 |
||
UPPS | Escherichia coli | IPP | 23 | µM | 1.5 | s^-1 | 25°C | 7.5 | W207F | FPP | 11,744,728 |
||
UPPS | Escherichia coli | FPP | 0.5 | µM | 3.5 | s^-1 | 25°C | 7.5 | W221F | IPP | 11,744,728 |
||
UPPS | Escherichia coli | IPP | 14 | µM | 3.5 | s^-1 | 25°C | 7.5 | W221F | FPP | 11,744,728 |
||
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 46 | mM | 17.6 | s^-1 | 0.38 | mM^-1s^-1 | 95°C | 5.0 | Wild-type | hexanol | 11,745,143 |
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 46 | mM | 10.7 | s^-1 | 0.23 | mM^-1s^-1 | 95°C | 5.0 | Wild-type (transglucosylation) | hexanol | 11,745,143 |
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 49 | mM | 7.1 | s^-1 | 0.14 | mM^-1s^-1 | 95°C | 5.0 | Wild-type (hydrolysis) | hexanol | 11,745,143 |
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 28 | mM | 13.5 | s^-1 | 0.48 | mM^-1s^-1 | 95°C | 5.0 | M424K | hexanol | 11,745,143 |
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 24 | mM | 8.6 | s^-1 | 0.36 | mM^-1s^-1 | 95°C | 5.0 | M424K (transglucosylation) | hexanol | 11,745,143 |
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 39 | mM | 5.0 | s^-1 | 0.13 | mM^-1s^-1 | 95°C | 5.0 | M424K (hydrolysis) | hexanol | 11,745,143 |
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 19 | mM | 4.4 | s^-1 | 0.23 | mM^-1s^-1 | 95°C | 5.0 | F426Y | hexanol | 11,745,143 |
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 19 | mM | 3.4 | s^-1 | 0.18 | mM^-1s^-1 | 95°C | 5.0 | F426Y (transglucosylation) | hexanol | 11,745,143 |
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 21 | mM | 0.9 | s^-1 | 0.04 | mM^-1s^-1 | 95°C | 5.0 | F426Y (hydrolysis) | hexanol | 11,745,143 |
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 32 | mM | 3.9 | s^-1 | 0.12 | mM^-1s^-1 | 95°C | 5.0 | M424K/F426Y | hexanol | 11,745,143 |
β-glucosidase CelB | Pyrococcus furiosus | pentyl-β-glucoside | 34 | mM | 3.1 | s^-1 | 0.09 | mM^-1s^-1 | 95°C | 5.0 | M424K/F426Y (transglucosylation) | hexanol | 11,745,143 |
YcjG | Escherichia coli | L-Ala-D-Glu | null | M | 10 | s^-1 | 7.7 × 10^4 | M^-1s^-1 | 30°C | 8.5 | 11,747,447 |
||
YcjG | Escherichia coli | L-Ala-D-Asp | null | M | 17 | s^-1 | 8.9 × 10^4 | M^-1s^-1 | 30°C | 8.5 | 11,747,447 |
||
YcjG | Escherichia coli | L-Ala-D-Met | null | M | 1.9 | s^-1 | 2.8 × 10^3 | M^-1s^-1 | 30°C | 8.5 | 11,747,447 |
||
YcjG | Escherichia coli | L-Ala-D-Gln | null | M | 3.3 | s^-1 | 1.8 × 10^3 | M^-1s^-1 | 30°C | 8.5 | 11,747,447 |
||
YkfB | Bacillus subtilis | L-Ala-D-Glu | null | M | 15 | s^-1 | 4.7 × 10^4 | M^-1s^-1 | 30°C | 8.5 | 11,747,447 |
||
YkfB | Bacillus subtilis | L-Ala-D-Asp | null | M | 0.053 | s^-1 | 1.9 × 10^3 | M^-1s^-1 | 30°C | 8.5 | 11,747,447 |
||
YkfB | Bacillus subtilis | L-Ala-D-Met | null | M | 1.1 | s^-1 | 2.2 × 10^3 | M^-1s^-1 | 30°C | 8.5 | 11,747,447 |
||
YkfC | Bacillus subtilis | L-Ala-D-Glu-L-Lys-D-Ala-D-Ala | null | M | 5.7 | s^-1 | 1.8 × 10^4 | M^-1s^-1 | 30°C | 8.5 | 11,747,447 |
||
YkfC | Bacillus subtilis | L-Ala-D-Glu-L-Lys-D-Ala | null | M | 2.6 | s^-1 | 2.2 × 10^4 | M^-1s^-1 | 30°C | 8.5 | 11,747,447 |
||
SSβG | Sulfolobus solfataricus | 4-MUGlc | 0.046 | mM | 140 | s^-1 | 2900 | s^-1mM^-1 | 80°C | 6.5 | WT | 11,749,955 |
|
SSβG | Sulfolobus solfataricus | 4-MUGlc | 0.34 | mM | 5.1 | s^-1 | 15 | s^-1mM^-1 | 80°C | 6.5 | E432C | 11,749,955 |
|
SSβG | Sulfolobus solfataricus | 4-MUGlc | 1.61 | mM | 33 | s^-1 | 20 | s^-1mM^-1 | 80°C | 6.5 | W433C | 11,749,955 |
|
SSβG | Sulfolobus solfataricus | 4-MUGlc | 0.068 | mM | 190 | s^-1 | 2900 | s^-1mM^-1 | 80°C | 6.5 | M439C | 11,749,955 |
|
SSβG | Sulfolobus solfataricus | 4-MUGal | 0.066 | mM | 98 | s^-1 | 1490 | s^-1mM^-1 | 80°C | 6.5 | WT | 11,749,955 |