Enzyme
stringlengths 4
59
| Organism
stringlengths 1
70
| Substrate
stringlengths 1
86
| km
float64 0
1.4M
⌀ | Unit_Km
stringclasses 65
values | Kcat
stringlengths 1
16
| Unit_Kcat
stringclasses 134
values | Kcat/Km
stringlengths 1
21
| Unit_Kcat/Km
stringclasses 335
values | Commentary[Temp]
stringclasses 165
values | Commentary[pH]
stringclasses 225
values | Commentary[Mutant]
stringlengths 1
93
| Commentary[Cosubstrate]
stringlengths 1
89
| pubmed id
float64 1.5M
35.5M
⌀ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | NADPH | 17 | µM | 6.8 | s^-1 | 4.0 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | R18A | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribitol 5-phosphate | 1,000 | µM | 0.14 | s^-1 | 0.0014 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | R18A | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | CTP | 390 | µM | 0.14 | s^-1 | 0.0036 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | R18A | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribulose 5-phosphate | 190 | µM | 0.32 | s^-1 | 0.017 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | K386A | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | NADPH | 39 | µM | 0.32 | s^-1 | 0.082 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | K386A | 11,305,920 |
|
CDP-ribitol synthase (Bcs1) | Haemophilus influenzae | D-ribitol 5-phosphate | 120 | µM | 9.7 | s^-1 | 0.81 × 10^5 | M^-1 s^-1 | 25°C | 7.2 | K386A | 11,305,920 |
|
AdhA | Pyrococcus furiosus | Pyruvaldehyde | 3.3 | mM | 14.0 | s^-1 | 4.2 | s^-1·mM^-1 | 70°C | 7.0 | Wild-type | NADPH | 11,358,525 |
AdhA | Pyrococcus furiosus | NADPH | 0.08 | mM | 14.0 | s^-1 | 175.0 | s^-1·mM^-1 | 70°C | 7.0 | Wild-type | Pyruvaldehyde | 11,358,525 |
AdhA | Pyrococcus furiosus | 2-Pentanol | 60.8 | mM | 20.2 | s^-1 | 0.3 | s^-1·mM^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
AdhA | Pyrococcus furiosus | NADP | 0.08 | mM | 20.2 | s^-1 | 252.5 | s^-1·mM^-1 | 70°C | 10.5 | Wild-type | 2-Pentanol | 11,358,525 |
AdhA | Pyrococcus furiosus | Methanol | null | 0.7 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Ethanol | null | 0.7 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1-Propanol | null | 2.1 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1-Butanol | null | 5.1 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1-Pentanol | null | 9.8 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1-Hexanol | null | 13.2 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1-Heptanol | null | 14.5 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1-Octanol | null | 14.0 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1-Nonanol | null | 11.4 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1-Decanol | null | 7.3 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 2-Propanol | null | 10.4 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 2-Butanol | null | 16.8 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 2-Pentanol | null | 41.6 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 2-Hexanol | null | 39.6 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 2-Heptanol | null | 34.8 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 2-Octanol | null | 27.1 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Ethanediol | null | 1.0 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1,2-Propanediol | null | 2.9 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Glycerol | null | 0.2 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 2-Methyl-1-propanol | null | 6.2 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 2-Methyl-2-propanol | null | 0.2 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1,2-Butanol | null | 2.4 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 1,3-Butanol | null | 6.9 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | 2,3-Butanol | null | 5.5 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | (S)-2-Butanol | null | 32.3 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | (R)-2-Butanol | null | 21.8 | U·mg^-1 | 70°C | 10.5 | Wild-type | NADP | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Pyruvaldehyde | null | 30.2 | U·mg^-1 | 70°C | 7.0 | Wild-type | NADPH | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Acetoin | null | 3.2 | U·mg^-1 | 70°C | 7.0 | Wild-type | NADPH | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Propionaldehyde | null | 2.6 | U·mg^-1 | 70°C | 7.0 | Wild-type | NADPH | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Glyceraldehyde | null | 2.6 | U·mg^-1 | 70°C | 7.0 | Wild-type | NADPH | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Pyruvate | null | 1.4 | U·mg^-1 | 70°C | 7.0 | Wild-type | NADPH | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Propionate | null | 0.7 | U·mg^-1 | 70°C | 7.0 | Wild-type | NADPH | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Acetone | null | 0.2 | U·mg^-1 | 70°C | 7.0 | Wild-type | NADPH | 11,358,525 |
|||
AdhA | Pyrococcus furiosus | Hydroxyacetone | null | 1.3 | U·mg^-1 | 70°C | 7.0 | Wild-type | NADPH | 11,358,525 |
|||
Glutathione amide reductase | Chromatium gracile | GASSAG | 97 | µM | 14981 | min^-1 | 2.6 × 10^6 | M^-1s^-1 | 25°C | 7.1 | NADH 100 µM | 11,399,772 |
|
Glutathione amide reductase | Chromatium gracile | NADH | 13.2 | µM | 11210 | min^-1 | 14.2 × 10^6 | M^-1s^-1 | 25°C | 7.1 | GASSAG 500 µM | 11,399,772 |
|
Glutathione amide reductase | Chromatium gracile | Glutathione disulfide | 6,900 | µM | 8759 | min^-1 | 0.021 × 10^6 | M^-1s^-1 | 25°C | 7.1 | 11,399,772 |
||
Glutathione amide reductase | Chromatium gracile | NADPH | 1,980 | µM | 805 | min^-1 | 0.0068 × 10^6 | M^-1s^-1 | 25°C | 7.1 | 11,399,772 |
||
Glutathione reductase | Homo sapiens | Glutathione disulfide | 80 | µM | 11345 | min^-1 | 2.37 × 10^6 | M^-1s^-1 | 11,399,772 |
||||
Glutathione reductase | Homo sapiens | GASSAG | 2,300 | µM | 3744 | min^-1 | 0.027 × 10^6 | M^-1s^-1 | 11,399,772 |
||||
Glutathione reductase | Homo sapiens | NADPH | 21.7 | µM | 16000 | min^-1 | 12.3 × 10^6 | M^-1s^-1 | 11,399,772 |
||||
Mj0226 | Methanococcus jannaschii | XTP | 0.22 | mM | 176.40 | s^-1 | 801.81 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | dITP | 0.25 | mM | 152.44 | s^-1 | 609.76 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | ITP | 0.24 | mM | 155.78 | s^-1 | 649.08 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | dGTP | 2.12 | mM | 9.33 | s^-1 | 4.4 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | GTP | 1.94 | mM | 9.11 | s^-1 | 4.7 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | 3'dGTP | 2.87 | mM | 8.22 | s^-1 | 2.86 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | ddGTP | 2.78 | mM | 3.33 | s^-1 | 1.2 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | dATP | 7.3 | mM | 0.44 | s^-1 | 0.06 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | dCTP | 5.47 | mM | 0.44 | s^-1 | 0.08 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | dTTP | 4.4 | mM | 1.78 | s^-1 | 0.4 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | dUTP | 4.22 | mM | 5.78 | s^-1 | 1.37 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | 8-oxo-dGTP | 5.3 | mM | 2.44 | s^-1 | 0.46 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | 8-Br-dGTP | 6.1 | mM | 5.55 | s^-1 | 0.91 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | GMPPNP | 3.26 | mM | 0.67 | s^-1 | 0.2 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | GTP-γ-S | 3.71 | mM | 0.45 | s^-1 | 0.12 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | 6-Cl-PuTP | 12.4 | mM | 0.89 | s^-1 | 0.07 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | dHAPTP | 3.3 | mM | 1.78 | s^-1 | 0.54 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | 2-amino-6-Cl-PuTP | 15.2 | mM | 0.67 | s^-1 | 0.04 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | 2-amino-PuTP | 24.2 | mM | 0.44 | s^-1 | 0.02 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | 2,6-diamino-PuTP | 10.8 | mM | 0.44 | s^-1 | 0.04 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | Purine TP | 29 | mM | 0.22 | s^-1 | 0.01 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Mj0226 | Methanococcus jannaschii | Etheno-dATP | 8.2 | mM | 0.67 | s^-1 | 0.08 | mM^-1s^-1 | 80°C | 10.5 | WT | Mg^2+ | 11,452,035 |
Amidase | Sulfolobus solfataricus | Acetamide | 3.6 | mM | 1.6 | s^-1 | 4.4 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Propionamide | 1.3 | mM | 3.2 | s^-1 | 24.6 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Lactamide | 3 | mM | 10.7 | s^-1 | 35.7 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Butyramide | 1.2 | mM | 9.9 | s^-1 | 82.5 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Isobutyramide | 1 | mM | 21.9 | s^-1 | 219.0 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Pentanamide | 1.3 | mM | 20.5 | s^-1 | 157.7 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Hexanamide | 5.8 | mM | 5.3 | s^-1 | 9.1 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Cyclohexanamide | 5 | mM | 16.3 | s^-1 | 32.6 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Acrylamide | 3 | mM | 8.3 | s^-1 | 27.7 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Metacrylamide | 1.2 | mM | 2.9 | s^-1 | 24.2 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Prolinamide | 6.7 | mM | 3.1 | s^-1 | 4.6 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Benzamide | 0.3 | mM | 6.4 | s^-1 | 213.3 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | o-OH benzamide | 0.4 | mM | 1.3 | s^-1 | 32.5 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | p-OH benzamide | 1.5 | mM | 1.1 | s^-1 | 7.3 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | o-NH2 benzamide | 0.3 | mM | 0.9 | s^-1 | 30.0 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | p-NH2 benzamide | 0.3 | mM | 0.8 | s^-1 | 26.6 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | o-Toluamide | 0.3 | mM | 0.3 | s^-1 | 7.5 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | p-Toluamide | 0.3 | mM | 7.3 | s^-1 | 243.3 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Nicotinamide | 0.3 | mM | 1.6 | s^-1 | 53.3 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Isonicotinamide | 0.8 | mM | 1.6 | s^-1 | 20.0 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Picolinamide | 2.5 | mM | 1.8 | s^-1 | 7.2 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | 3-Phenylpropionamide | 4 | mM | 7.1 | s^-1 | 17.7 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
Amidase | Sulfolobus solfataricus | Indol-3-acetamide | 0.9 | mM | 1.8 | s^-1 | 20.0 | M^-1s^-1 × 10^2 | 70°C | 7.5 | 11,453,462 |
||
YgdP | Escherichia coli K1 | Ap5A | 0.36 | mM | 1 | s^-1 | 2.78 | mM^-1s^-1 | 37°C | 9.0 | WT | Mg^2+ | 11,479,323 |
YgdP | Escherichia coli K1 | Ap4A | 0.07 | mM | 0.1 | s^-1 | 1.43 | mM^-1s^-1 | 37°C | 9.0 | WT | Mg^2+ | 11,479,323 |
IalA | Bartonella bacilliformis | Ap4A | null | 37°C | WT | 11,479,323 |
|||||||
IalA | Bartonella bacilliformis | Ap5A | null | 37°C | WT | 11,479,323 |