Enzyme
stringlengths
4
59
Organism
stringlengths
1
70
Substrate
stringlengths
1
86
km
float64
0
1.4M
Unit_Km
stringclasses
65 values
Kcat
stringlengths
1
16
Unit_Kcat
stringclasses
134 values
Kcat/Km
stringlengths
1
21
Unit_Kcat/Km
stringclasses
335 values
Commentary[Temp]
stringclasses
165 values
Commentary[pH]
stringclasses
225 values
Commentary[Mutant]
stringlengths
1
93
Commentary[Cosubstrate]
stringlengths
1
89
pubmed id
float64
1.5M
35.5M
Selenocysteine methyltransferase
Astragalus bisulcatus
L-Selenocysteine
0.7
mM
6.2 × 10^-3
s^-1
8.9
M^-1s^-1
S-adenosylmethionine
10,026,151
Selenocysteine methyltransferase
Astragalus bisulcatus
DL-Selenohomocysteine
0.35
mM
7.4 × 10^-3
s^-1
21
M^-1s^-1
S-adenosylmethionine
10,026,151
Homocysteine methyltransferase (YagD)
Escherichia coli
L-Homocysteine
0.045
mM
0.38
s^-1
8.5 × 10^3
M^-1s^-1
S-adenosylmethionine
10,026,151
Homocysteine methyltransferase (YagD)
Escherichia coli
L-Selenocysteine
0.59
mM
1.7 × 10^-4
s^-1
0.29
M^-1s^-1
S-adenosylmethionine
10,026,151
LpxC
Escherichia coli
UDP-3-O-acyl-GlcNAc
0.6
µM
0.09
s^-1
0.14 × 10^6
M^-1s^-1
1°C
5.7
10,026,271
LpxC
Escherichia coli
UDP-3-O-acyl-GlcNAc
2.1
µM
3.3
s^-1
1.5 × 10^6
M^-1s^-1
30°C
5.5
10,026,271
LpxC
Escherichia coli
UDP-GlcNAc
20,000
mM
0.006
s^-1
0.28
M^-1s^-1
30°C
5.5
10,026,271
LpxC
Escherichia coli
UDP-3-O-acyl-GlcNAc
null
1°C
6.0
5 µM EDTA
10,026,271
LpxC
Escherichia coli
UDP-3-O-acyl-GlcNAc
null
1°C
6.0
25 µM DPA
10,026,271
LpxC
Escherichia coli
UDP-3-O-acyl-GlcNAc
null
1°C
6.0
7 µM ZnSO4
10,026,271
LpxC
Escherichia coli
UDP-3-O-acyl-GlcNAc
null
30°C
5.5
24 µM ZnSO4
10,026,271
LpxC
Escherichia coli
UDP-GlcNAc
null
30°C
5.7
Apo-LpxC
10,026,271
LpxC
Escherichia coli
UDP-GlcNAc
null
30°C
5.7
Zn2+-reconstituted
10,026,271
LpxC
Escherichia coli
UDP-GlcNAc
null
30°C
5.7
Cd2+-reconstituted
10,026,271
LpxC
Escherichia coli
UDP-GlcNAc
null
30°C
5.7
Ca2+-reconstituted
10,026,271
LpxC
Escherichia coli
UDP-GlcNAc
null
30°C
5.7
Cu2+-reconstituted
10,026,271
LpxC
Escherichia coli
UDP-GlcNAc
null
30°C
5.7
Mg2+-reconstituted
10,026,271
LpxC
Escherichia coli
UDP-GlcNAc
null
30°C
5.7
Mn2+-reconstituted
10,026,271
LpxC
Escherichia coli
UDP-GlcNAc
null
30°C
5.7
Ni2+-reconstituted
10,026,271
Aps1
Schizosaccharomyces pombe
Ap6A
19.1
µM
2.0
s^-1
1.0 × 10^5
M^-1s^-1
37°C
7.6
1 mM MnCl2
10,090,752
Pus1
Saccharomyces cerevisiae
tRNA^Val
420
nM
0.4
min^-1
10,356,324
Pus1
Saccharomyces cerevisiae
tRNA^Ile
740
nM
0.5
min^-1
10,356,324
hMTH1
Homo sapiens
2-OH-dATP
8.3
µM
13.9
s^-1
1.68
s^-1·µM^-1
30°C
8.0
10,373,420
hMTH1
Homo sapiens
8-OH-dGTP
15.2
µM
12.3
s^-1
0.81
s^-1·µM^-1
30°C
8.0
10,373,420
hMTH1
Homo sapiens
8-OH-dATP
13.9
µM
10.8
s^-1
0.78
s^-1·µM^-1
30°C
8.0
10,373,420
hMTH1
Homo sapiens
dGTP
258
µM
15.7
s^-1
0.06
s^-1·µM^-1
30°C
8.0
10,373,420
hMTH1
Homo sapiens
2-OH-dATP
5.7
µM
4.7
s^-1
0.83
s^-1·µM^-1
30°C
7.2
10,373,420
hMTH1
Homo sapiens
8-OH-dGTP
12.8
µM
2.1
s^-1
0.16
s^-1·µM^-1
30°C
7.2
10,373,420
hMTH1
Homo sapiens
8-OH-dGTP
null
30°C
7.2-8.8
10,373,420
hMTH1
Homo sapiens
2-OH-dATP
null
30°C
7.2-8.8
10,373,420
MutT
Escherichia coli
8-OH-dGTP
null
30°C
7.5
10,373,420
MutT
Escherichia coli
dGTP
null
30°C
7.5
10,373,420
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
1
µM
6.0
s^-1
6.0
µM^-1s^-1
28°C
7.8
WT
5 mM MgCl2
10,383,414
Tyrosyl-tRNA synthetase
Escherichia coli
D-Tyr-tRNA^Tyr
0.2
µM
1.5 × 10^-3
s^-1
7.5 × 10^-3
µM^-1s^-1
28°C
7.8
WT
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
None
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
5 mM MgCl2
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
10 mM MgCl2
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
20 mM MgCl2
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
50 mM KCl
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
200 mM KCl
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
400 mM KCl
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
5 mM MgCl2 + 50 mM KCl
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
5 mM MgCl2 + 0.1 mM ZnCl2
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
1 mM spermidine
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
5 mM spermidine
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
28°C
7.8
WT
10 mM spermidine
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
D-Tyr-tRNA^Tyr
null
6.0
µM^-1s^-1
28°C
7.8
WT
5 mM MgCl2
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
L-Tyr-tRNA^Tyr
null
< 10^-3
µM^-1s^-1
28°C
7.8
WT
5 mM MgCl2
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
N-Acetyl-D-Tyr-tRNA^Tyr
null
< 10^-3
µM^-1s^-1
28°C
7.8
WT
5 mM MgCl2
10,383,414
D-Tyr-tRNA^Tyr deacylase
Escherichia coli
N-Acetyl-L-Tyr-tRNA^Tyr
null
< 10^-3
µM^-1s^-1
28°C
7.8
WT
5 mM MgCl2
10,383,414
Peptidyl-tRNA hydrolase
Escherichia coli
D-Tyr-tRNA^Tyr
null
0.016
µM^-1s^-1
28°C
7.8
WT
5 mM MgCl2
10,383,414
Peptidyl-tRNA hydrolase
Escherichia coli
L-Tyr-tRNA^Tyr
null
0.006
µM^-1s^-1
28°C
7.8
WT
5 mM MgCl2
10,383,414
Peptidyl-tRNA hydrolase
Escherichia coli
N-Acetyl-D-Tyr-tRNA^Tyr
null
0.003
µM^-1s^-1
28°C
7.8
WT
5 mM MgCl2
10,383,414
Mj0226
Methanococcus jannaschii
XTP
0.1
mM
1009.37
s^-1
10195.66
mM^-1s^-1
80°C
7.6
WT
Mg^2+
10,404,228
Mj0226
Methanococcus jannaschii
ITP
0.15
mM
911.72
s^-1
5998.16
mM^-1s^-1
80°C
7.6
WT
Mg^2+
10,404,228
Mj0226
Methanococcus jannaschii
GTP
1.11
mM
97.65
s^-1
87.66
mM^-1s^-1
80°C
7.6
WT
Mg^2+
10,404,228
Mj0226
Methanococcus jannaschii
dGTP
1.13
mM
96.64
s^-1
85.52
mM^-1s^-1
80°C
7.6
WT
Mg^2+
10,404,228
Mj0226
Methanococcus jannaschii
ATP
7.04
mM
1.02
s^-1
0.15
mM^-1s^-1
80°C
7.6
WT
Mg^2+
10,404,228
Mj0226
Methanococcus jannaschii
CTP
1.45
mM
2.23
s^-1
1.54
mM^-1s^-1
80°C
7.6
WT
Mg^2+
10,404,228
Mj0226
Methanococcus jannaschii
TTP
0.3
mM
1.77
s^-1
5.90
mM^-1s^-1
80°C
7.6
WT
Mg^2+
10,404,228
β-Glycosidase
Sulfolobus solfataricus
o-nph-β-D-Galp
1
mM
24
s^-1
24
s^-1/mM
30°C
7.4
10,467,170
β-Glycosidase
Sulfolobus solfataricus
o-nph-β-D-Galp
0.5
mM
55
s^-1
110
s^-1/mM
30°C
7.4
80 mM 1-butanol
10,467,170
β-Glycosidase
Sulfolobus solfataricus
o-nph-β-D-Galp
4
mM
1320
s^-1
330
s^-1/mM
75°C
7.4
10,467,170
β-Glycosidase
Sulfolobus solfataricus
o-nph-β-D-Galp
11
mM
1450
s^-1
131.88
s^-1/mM
75°C
7.4
80 mM 1-butanol
10,467,170
SsuE
Escherichia coli
FMN
0.054
µM
474.1
min^-1µM^-1
30°C
7.9
NADH
10,480,865
SsuE
Escherichia coli
FAD
2.7
µM
16.9
min^-1µM^-1
30°C
7.9
NADH
10,480,865
SsuE
Escherichia coli
NADPH
46
µM
33.4
min^-1µM^-1
30°C
7.9
FMN
10,480,865
SsuE
Escherichia coli
NADH
555.5
µM
0.1
min^-1µM^-1
30°C
7.9
FMN
10,480,865
SsuD
Escherichia coli
Decanesulfonic acid
35
µM
6.7
min^-1µM^-1
30°C
9.1
FMNH2, O2
10,480,865
SsuD
Escherichia coli
Octanesulfonic acid
44
µM
6.1
min^-1µM^-1
30°C
9.1
FMNH2, O2
10,480,865
SsuD
Escherichia coli
1,3-Dioxo-2-isoindolineethanesulfonic acid
114
µM
6.0
min^-1µM^-1
30°C
9.1
FMNH2, O2
10,480,865
SsuD
Escherichia coli
2-(4-Pyridyl)ethanesulfonic acid
139
µM
4.6
min^-1µM^-1
30°C
9.1
FMNH2, O2
10,480,865
SsuD
Escherichia coli
Hexanesulfonic acid
95
µM
4.0
min^-1µM^-1
30°C
9.1
FMNH2, O2
10,480,865
SsuD
Escherichia coli
N-Phenyltaurine
237
µM
3.2
min^-1µM^-1
30°C
9.1
FMNH2, O2
10,480,865
SsuD
Escherichia coli
4-Phenyl-1-butanesulfonic acid
110
µM
2.7
min^-1µM^-1
30°C
9.1
FMNH2, O2
10,480,865
SsuD
Escherichia coli
Pentanesulfonic acid
189
µM
1.8
min^-1µM^-1
30°C
9.1
FMNH2, O2
10,480,865
SsuD
Escherichia coli
MOPS
617
µM
1.1
min^-1µM^-1
30°C
9.1
FMNH2, O2
10,480,865
SsuD
Escherichia coli
Butanesulfonic acid
870
µM
0.6
min^-1µM^-1
30°C
9.1
FMNH2, O2
10,480,865
4-oxalocrotonate tautomerase
Pseudomonas putida mt-2
2-hydroxy-2,4-hexadienedioate
0.18
mM
3500
s^-1
1.9 × 10^7
M^-1s^-1
23°C
7.3
WT
10,493,802
4-oxalocrotonate tautomerase
Pseudomonas putida mt-2
2-hydroxy-2,4-hexadienedioate
1.6
mM
40
s^-1
2.5 × 10^4
M^-1s^-1
23°C
7.3
R11A
10,493,802
4-oxalocrotonate tautomerase
Pseudomonas putida mt-2
2-hydroxy-2,4-hexadienedioate
0.29
mM
28
s^-1
9.7 × 10^4
M^-1s^-1
23°C
7.3
R39A
10,493,802
4-oxalocrotonate tautomerase
Pseudomonas putida mt-2
2-hydroxy-2,4-hexadienedioate
0.47
mM
9.0
s^-1
1.9 × 10^4
M^-1s^-1
23°C
7.3
R39Q
10,493,802
4-oxalocrotonate tautomerase
Pseudomonas putida mt-2
2-hydroxy-2,4-hexadienedioate
null
≤0.4
s^-1
23°C
7.3
R11A/R39A
10,493,802
4-oxalocrotonate tautomerase
Pseudomonas putida mt-2
2-hydroxy-2,4-hexadienedioate
0.29
mM
3500
s^-1
1.2 × 10^7
M^-1s^-1
23°C
7.3
R61A
10,493,802
4-oxalocrotonate tautomerase
Pseudomonas putida mt-2
2-hydroxy-2,4-pentadienoate
1.11
mM
0.40
s^-1
3.6 × 10^2
M^-1s^-1
23°C
7.3
WT
10,493,802
TruB
Escherichia coli
[5-3H]tRNA
146
nM
0.12
s^-1
7.5 × 10^5
M^-1s^-1
37°C
7.5
Wild-type
10,529,181
TruB
Escherichia coli
[5-3H]tRNA
177
nM
0.26
s^-1
1.5 × 10^6
M^-1s^-1
37°C
7.5
C58A
10,529,181
TruB
Escherichia coli
[5-3H]tRNA
127
nM
0.16
s^-1
1.3 × 10^6
M^-1s^-1
37°C
7.5
C174A
10,529,181
TruB
Escherichia coli
[5-3H]tRNA
218
nM
0.18
s^-1
8.3 × 10^5
M^-1s^-1
37°C
7.5
C193V
10,529,181
TruB
Escherichia coli
[5-3H]tRNA
124
nM
0.11
s^-1
8.9 × 10^5
M^-1s^-1
37°C
7.5
C58A/C174A/C193A
10,529,181
RluA
Escherichia coli
[5-3H]tRNA
108
nM
0.099
s^-1
9.2 × 10^5
M^-1s^-1
37°C
7.5
Wild-type
10,529,181
RluA
Escherichia coli
[5-3H]tRNA
420
nM
0.070
s^-1
1.7 × 10^5
M^-1s^-1
37°C
7.5
C117A
10,529,181
RluA
Escherichia coli
[5-3H]tRNA
160
nM
0.15
s^-1
9.4 × 10^5
M^-1s^-1
37°C
7.5
C128A
10,529,181
Catalase-peroxidase
Synechocystis PCC 6803
H2O2
4.9
mM
3500
s^-1
7.1 × 10^5
M^-1s^-1
7.0
Recombinant
10,543,446
Catalase-peroxidase
Synechocystis PCC 6803
Peroxoacetic acid
null
8.74 × 10^3
M^-1s^-1
15°C
7.0
Recombinant
10,543,446
Catalase-peroxidase
Synechocystis PCC 6803
o-dianisidine
null
2.71 × 10^6
M^-1s^-1
15°C
7.0
Recombinant
10,543,446
Catalase-peroxidase
Synechocystis PCC 6803
Pyrogallol
null
8.62 × 10^4
M^-1s^-1
15°C
7.0
Recombinant
10,543,446
Catalase-peroxidase
Synechocystis PCC 6803
Ascorbate
null
5.43 × 10^3
M^-1s^-1
15°C
7.0
Recombinant
10,543,446
Catalase-peroxidase
Synechocystis PCC 6803
NaCN
null
4.8 × 10^5
M^-1s^-1
Recombinant
10,543,446
PQQGDH-B
Acinetobacter calcoaceticus
Glucose
26.8
mM
3436
s^-1
128
s^-1 mM^-1
Wild type
10,544,015

🚩Citation

Please cite the following paper if you use jackkuo/LLM-Enzyme-Kinetics-Archive-LLENKA in your work.

@INPROCEEDINGS{
  author={coming soon},
  title={Leveraging Large Language Models for Automated Extraction of Enzyme Kinetics Data from Scientific Literature}, 
  year={2024},
  volume={},
  number={},
  doi={coming soon}}
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