Enzyme
stringlengths 4
59
| Organism
stringlengths 1
70
| Substrate
stringlengths 1
86
| km
float64 0
1.4M
⌀ | Unit_Km
stringclasses 65
values | Kcat
stringlengths 1
16
| Unit_Kcat
stringclasses 134
values | Kcat/Km
stringlengths 1
21
| Unit_Kcat/Km
stringclasses 335
values | Commentary[Temp]
stringclasses 165
values | Commentary[pH]
stringclasses 225
values | Commentary[Mutant]
stringlengths 1
93
| Commentary[Cosubstrate]
stringlengths 1
89
| pubmed id
float64 1.5M
35.5M
⌀ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Selenocysteine methyltransferase | Astragalus bisulcatus | L-Selenocysteine | 0.7 | mM | 6.2 × 10^-3 | s^-1 | 8.9 | M^-1s^-1 | S-adenosylmethionine | 10,026,151 |
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Selenocysteine methyltransferase | Astragalus bisulcatus | DL-Selenohomocysteine | 0.35 | mM | 7.4 × 10^-3 | s^-1 | 21 | M^-1s^-1 | S-adenosylmethionine | 10,026,151 |
|||
Homocysteine methyltransferase (YagD) | Escherichia coli | L-Homocysteine | 0.045 | mM | 0.38 | s^-1 | 8.5 × 10^3 | M^-1s^-1 | S-adenosylmethionine | 10,026,151 |
|||
Homocysteine methyltransferase (YagD) | Escherichia coli | L-Selenocysteine | 0.59 | mM | 1.7 × 10^-4 | s^-1 | 0.29 | M^-1s^-1 | S-adenosylmethionine | 10,026,151 |
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LpxC | Escherichia coli | UDP-3-O-acyl-GlcNAc | 0.6 | µM | 0.09 | s^-1 | 0.14 × 10^6 | M^-1s^-1 | 1°C | 5.7 | 10,026,271 |
||
LpxC | Escherichia coli | UDP-3-O-acyl-GlcNAc | 2.1 | µM | 3.3 | s^-1 | 1.5 × 10^6 | M^-1s^-1 | 30°C | 5.5 | 10,026,271 |
||
LpxC | Escherichia coli | UDP-GlcNAc | 20,000 | mM | 0.006 | s^-1 | 0.28 | M^-1s^-1 | 30°C | 5.5 | 10,026,271 |
||
LpxC | Escherichia coli | UDP-3-O-acyl-GlcNAc | null | 1°C | 6.0 | 5 µM EDTA | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-3-O-acyl-GlcNAc | null | 1°C | 6.0 | 25 µM DPA | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-3-O-acyl-GlcNAc | null | 1°C | 6.0 | 7 µM ZnSO4 | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-3-O-acyl-GlcNAc | null | 30°C | 5.5 | 24 µM ZnSO4 | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-GlcNAc | null | 30°C | 5.7 | Apo-LpxC | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-GlcNAc | null | 30°C | 5.7 | Zn2+-reconstituted | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-GlcNAc | null | 30°C | 5.7 | Cd2+-reconstituted | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-GlcNAc | null | 30°C | 5.7 | Ca2+-reconstituted | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-GlcNAc | null | 30°C | 5.7 | Cu2+-reconstituted | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-GlcNAc | null | 30°C | 5.7 | Mg2+-reconstituted | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-GlcNAc | null | 30°C | 5.7 | Mn2+-reconstituted | 10,026,271 |
||||||
LpxC | Escherichia coli | UDP-GlcNAc | null | 30°C | 5.7 | Ni2+-reconstituted | 10,026,271 |
||||||
Aps1 | Schizosaccharomyces pombe | Ap6A | 19.1 | µM | 2.0 | s^-1 | 1.0 × 10^5 | M^-1s^-1 | 37°C | 7.6 | 1 mM MnCl2 | 10,090,752 |
|
Pus1 | Saccharomyces cerevisiae | tRNA^Val | 420 | nM | 0.4 | min^-1 | 10,356,324 |
||||||
Pus1 | Saccharomyces cerevisiae | tRNA^Ile | 740 | nM | 0.5 | min^-1 | 10,356,324 |
||||||
hMTH1 | Homo sapiens | 2-OH-dATP | 8.3 | µM | 13.9 | s^-1 | 1.68 | s^-1·µM^-1 | 30°C | 8.0 | 10,373,420 |
||
hMTH1 | Homo sapiens | 8-OH-dGTP | 15.2 | µM | 12.3 | s^-1 | 0.81 | s^-1·µM^-1 | 30°C | 8.0 | 10,373,420 |
||
hMTH1 | Homo sapiens | 8-OH-dATP | 13.9 | µM | 10.8 | s^-1 | 0.78 | s^-1·µM^-1 | 30°C | 8.0 | 10,373,420 |
||
hMTH1 | Homo sapiens | dGTP | 258 | µM | 15.7 | s^-1 | 0.06 | s^-1·µM^-1 | 30°C | 8.0 | 10,373,420 |
||
hMTH1 | Homo sapiens | 2-OH-dATP | 5.7 | µM | 4.7 | s^-1 | 0.83 | s^-1·µM^-1 | 30°C | 7.2 | 10,373,420 |
||
hMTH1 | Homo sapiens | 8-OH-dGTP | 12.8 | µM | 2.1 | s^-1 | 0.16 | s^-1·µM^-1 | 30°C | 7.2 | 10,373,420 |
||
hMTH1 | Homo sapiens | 8-OH-dGTP | null | 30°C | 7.2-8.8 | 10,373,420 |
|||||||
hMTH1 | Homo sapiens | 2-OH-dATP | null | 30°C | 7.2-8.8 | 10,373,420 |
|||||||
MutT | Escherichia coli | 8-OH-dGTP | null | 30°C | 7.5 | 10,373,420 |
|||||||
MutT | Escherichia coli | dGTP | null | 30°C | 7.5 | 10,373,420 |
|||||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | 1 | µM | 6.0 | s^-1 | 6.0 | µM^-1s^-1 | 28°C | 7.8 | WT | 5 mM MgCl2 | 10,383,414 |
Tyrosyl-tRNA synthetase | Escherichia coli | D-Tyr-tRNA^Tyr | 0.2 | µM | 1.5 × 10^-3 | s^-1 | 7.5 × 10^-3 | µM^-1s^-1 | 28°C | 7.8 | WT | 10,383,414 |
|
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | None | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 5 mM MgCl2 | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 10 mM MgCl2 | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 20 mM MgCl2 | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 50 mM KCl | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 200 mM KCl | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 400 mM KCl | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 5 mM MgCl2 + 50 mM KCl | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 5 mM MgCl2 + 0.1 mM ZnCl2 | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 1 mM spermidine | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 5 mM spermidine | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 28°C | 7.8 | WT | 10 mM spermidine | 10,383,414 |
|||||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 6.0 | µM^-1s^-1 | 28°C | 7.8 | WT | 5 mM MgCl2 | 10,383,414 |
|||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | L-Tyr-tRNA^Tyr | null | < 10^-3 | µM^-1s^-1 | 28°C | 7.8 | WT | 5 mM MgCl2 | 10,383,414 |
|||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | N-Acetyl-D-Tyr-tRNA^Tyr | null | < 10^-3 | µM^-1s^-1 | 28°C | 7.8 | WT | 5 mM MgCl2 | 10,383,414 |
|||
D-Tyr-tRNA^Tyr deacylase | Escherichia coli | N-Acetyl-L-Tyr-tRNA^Tyr | null | < 10^-3 | µM^-1s^-1 | 28°C | 7.8 | WT | 5 mM MgCl2 | 10,383,414 |
|||
Peptidyl-tRNA hydrolase | Escherichia coli | D-Tyr-tRNA^Tyr | null | 0.016 | µM^-1s^-1 | 28°C | 7.8 | WT | 5 mM MgCl2 | 10,383,414 |
|||
Peptidyl-tRNA hydrolase | Escherichia coli | L-Tyr-tRNA^Tyr | null | 0.006 | µM^-1s^-1 | 28°C | 7.8 | WT | 5 mM MgCl2 | 10,383,414 |
|||
Peptidyl-tRNA hydrolase | Escherichia coli | N-Acetyl-D-Tyr-tRNA^Tyr | null | 0.003 | µM^-1s^-1 | 28°C | 7.8 | WT | 5 mM MgCl2 | 10,383,414 |
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Mj0226 | Methanococcus jannaschii | XTP | 0.1 | mM | 1009.37 | s^-1 | 10195.66 | mM^-1s^-1 | 80°C | 7.6 | WT | Mg^2+ | 10,404,228 |
Mj0226 | Methanococcus jannaschii | ITP | 0.15 | mM | 911.72 | s^-1 | 5998.16 | mM^-1s^-1 | 80°C | 7.6 | WT | Mg^2+ | 10,404,228 |
Mj0226 | Methanococcus jannaschii | GTP | 1.11 | mM | 97.65 | s^-1 | 87.66 | mM^-1s^-1 | 80°C | 7.6 | WT | Mg^2+ | 10,404,228 |
Mj0226 | Methanococcus jannaschii | dGTP | 1.13 | mM | 96.64 | s^-1 | 85.52 | mM^-1s^-1 | 80°C | 7.6 | WT | Mg^2+ | 10,404,228 |
Mj0226 | Methanococcus jannaschii | ATP | 7.04 | mM | 1.02 | s^-1 | 0.15 | mM^-1s^-1 | 80°C | 7.6 | WT | Mg^2+ | 10,404,228 |
Mj0226 | Methanococcus jannaschii | CTP | 1.45 | mM | 2.23 | s^-1 | 1.54 | mM^-1s^-1 | 80°C | 7.6 | WT | Mg^2+ | 10,404,228 |
Mj0226 | Methanococcus jannaschii | TTP | 0.3 | mM | 1.77 | s^-1 | 5.90 | mM^-1s^-1 | 80°C | 7.6 | WT | Mg^2+ | 10,404,228 |
β-Glycosidase | Sulfolobus solfataricus | o-nph-β-D-Galp | 1 | mM | 24 | s^-1 | 24 | s^-1/mM | 30°C | 7.4 | 10,467,170 |
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β-Glycosidase | Sulfolobus solfataricus | o-nph-β-D-Galp | 0.5 | mM | 55 | s^-1 | 110 | s^-1/mM | 30°C | 7.4 | 80 mM 1-butanol | 10,467,170 |
|
β-Glycosidase | Sulfolobus solfataricus | o-nph-β-D-Galp | 4 | mM | 1320 | s^-1 | 330 | s^-1/mM | 75°C | 7.4 | 10,467,170 |
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β-Glycosidase | Sulfolobus solfataricus | o-nph-β-D-Galp | 11 | mM | 1450 | s^-1 | 131.88 | s^-1/mM | 75°C | 7.4 | 80 mM 1-butanol | 10,467,170 |
|
SsuE | Escherichia coli | FMN | 0.054 | µM | 474.1 | min^-1µM^-1 | 30°C | 7.9 | NADH | 10,480,865 |
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SsuE | Escherichia coli | FAD | 2.7 | µM | 16.9 | min^-1µM^-1 | 30°C | 7.9 | NADH | 10,480,865 |
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SsuE | Escherichia coli | NADPH | 46 | µM | 33.4 | min^-1µM^-1 | 30°C | 7.9 | FMN | 10,480,865 |
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SsuE | Escherichia coli | NADH | 555.5 | µM | 0.1 | min^-1µM^-1 | 30°C | 7.9 | FMN | 10,480,865 |
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SsuD | Escherichia coli | Decanesulfonic acid | 35 | µM | 6.7 | min^-1µM^-1 | 30°C | 9.1 | FMNH2, O2 | 10,480,865 |
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SsuD | Escherichia coli | Octanesulfonic acid | 44 | µM | 6.1 | min^-1µM^-1 | 30°C | 9.1 | FMNH2, O2 | 10,480,865 |
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SsuD | Escherichia coli | 1,3-Dioxo-2-isoindolineethanesulfonic acid | 114 | µM | 6.0 | min^-1µM^-1 | 30°C | 9.1 | FMNH2, O2 | 10,480,865 |
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SsuD | Escherichia coli | 2-(4-Pyridyl)ethanesulfonic acid | 139 | µM | 4.6 | min^-1µM^-1 | 30°C | 9.1 | FMNH2, O2 | 10,480,865 |
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SsuD | Escherichia coli | Hexanesulfonic acid | 95 | µM | 4.0 | min^-1µM^-1 | 30°C | 9.1 | FMNH2, O2 | 10,480,865 |
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SsuD | Escherichia coli | N-Phenyltaurine | 237 | µM | 3.2 | min^-1µM^-1 | 30°C | 9.1 | FMNH2, O2 | 10,480,865 |
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SsuD | Escherichia coli | 4-Phenyl-1-butanesulfonic acid | 110 | µM | 2.7 | min^-1µM^-1 | 30°C | 9.1 | FMNH2, O2 | 10,480,865 |
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SsuD | Escherichia coli | Pentanesulfonic acid | 189 | µM | 1.8 | min^-1µM^-1 | 30°C | 9.1 | FMNH2, O2 | 10,480,865 |
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SsuD | Escherichia coli | MOPS | 617 | µM | 1.1 | min^-1µM^-1 | 30°C | 9.1 | FMNH2, O2 | 10,480,865 |
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SsuD | Escherichia coli | Butanesulfonic acid | 870 | µM | 0.6 | min^-1µM^-1 | 30°C | 9.1 | FMNH2, O2 | 10,480,865 |
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4-oxalocrotonate tautomerase | Pseudomonas putida mt-2 | 2-hydroxy-2,4-hexadienedioate | 0.18 | mM | 3500 | s^-1 | 1.9 × 10^7 | M^-1s^-1 | 23°C | 7.3 | WT | 10,493,802 |
|
4-oxalocrotonate tautomerase | Pseudomonas putida mt-2 | 2-hydroxy-2,4-hexadienedioate | 1.6 | mM | 40 | s^-1 | 2.5 × 10^4 | M^-1s^-1 | 23°C | 7.3 | R11A | 10,493,802 |
|
4-oxalocrotonate tautomerase | Pseudomonas putida mt-2 | 2-hydroxy-2,4-hexadienedioate | 0.29 | mM | 28 | s^-1 | 9.7 × 10^4 | M^-1s^-1 | 23°C | 7.3 | R39A | 10,493,802 |
|
4-oxalocrotonate tautomerase | Pseudomonas putida mt-2 | 2-hydroxy-2,4-hexadienedioate | 0.47 | mM | 9.0 | s^-1 | 1.9 × 10^4 | M^-1s^-1 | 23°C | 7.3 | R39Q | 10,493,802 |
|
4-oxalocrotonate tautomerase | Pseudomonas putida mt-2 | 2-hydroxy-2,4-hexadienedioate | null | ≤0.4 | s^-1 | 23°C | 7.3 | R11A/R39A | 10,493,802 |
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4-oxalocrotonate tautomerase | Pseudomonas putida mt-2 | 2-hydroxy-2,4-hexadienedioate | 0.29 | mM | 3500 | s^-1 | 1.2 × 10^7 | M^-1s^-1 | 23°C | 7.3 | R61A | 10,493,802 |
|
4-oxalocrotonate tautomerase | Pseudomonas putida mt-2 | 2-hydroxy-2,4-pentadienoate | 1.11 | mM | 0.40 | s^-1 | 3.6 × 10^2 | M^-1s^-1 | 23°C | 7.3 | WT | 10,493,802 |
|
TruB | Escherichia coli | [5-3H]tRNA | 146 | nM | 0.12 | s^-1 | 7.5 × 10^5 | M^-1s^-1 | 37°C | 7.5 | Wild-type | 10,529,181 |
|
TruB | Escherichia coli | [5-3H]tRNA | 177 | nM | 0.26 | s^-1 | 1.5 × 10^6 | M^-1s^-1 | 37°C | 7.5 | C58A | 10,529,181 |
|
TruB | Escherichia coli | [5-3H]tRNA | 127 | nM | 0.16 | s^-1 | 1.3 × 10^6 | M^-1s^-1 | 37°C | 7.5 | C174A | 10,529,181 |
|
TruB | Escherichia coli | [5-3H]tRNA | 218 | nM | 0.18 | s^-1 | 8.3 × 10^5 | M^-1s^-1 | 37°C | 7.5 | C193V | 10,529,181 |
|
TruB | Escherichia coli | [5-3H]tRNA | 124 | nM | 0.11 | s^-1 | 8.9 × 10^5 | M^-1s^-1 | 37°C | 7.5 | C58A/C174A/C193A | 10,529,181 |
|
RluA | Escherichia coli | [5-3H]tRNA | 108 | nM | 0.099 | s^-1 | 9.2 × 10^5 | M^-1s^-1 | 37°C | 7.5 | Wild-type | 10,529,181 |
|
RluA | Escherichia coli | [5-3H]tRNA | 420 | nM | 0.070 | s^-1 | 1.7 × 10^5 | M^-1s^-1 | 37°C | 7.5 | C117A | 10,529,181 |
|
RluA | Escherichia coli | [5-3H]tRNA | 160 | nM | 0.15 | s^-1 | 9.4 × 10^5 | M^-1s^-1 | 37°C | 7.5 | C128A | 10,529,181 |
|
Catalase-peroxidase | Synechocystis PCC 6803 | H2O2 | 4.9 | mM | 3500 | s^-1 | 7.1 × 10^5 | M^-1s^-1 | 7.0 | Recombinant | 10,543,446 |
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Catalase-peroxidase | Synechocystis PCC 6803 | Peroxoacetic acid | null | 8.74 × 10^3 | M^-1s^-1 | 15°C | 7.0 | Recombinant | 10,543,446 |
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Catalase-peroxidase | Synechocystis PCC 6803 | o-dianisidine | null | 2.71 × 10^6 | M^-1s^-1 | 15°C | 7.0 | Recombinant | 10,543,446 |
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Catalase-peroxidase | Synechocystis PCC 6803 | Pyrogallol | null | 8.62 × 10^4 | M^-1s^-1 | 15°C | 7.0 | Recombinant | 10,543,446 |
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Catalase-peroxidase | Synechocystis PCC 6803 | Ascorbate | null | 5.43 × 10^3 | M^-1s^-1 | 15°C | 7.0 | Recombinant | 10,543,446 |
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Catalase-peroxidase | Synechocystis PCC 6803 | NaCN | null | 4.8 × 10^5 | M^-1s^-1 | Recombinant | 10,543,446 |
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PQQGDH-B | Acinetobacter calcoaceticus | Glucose | 26.8 | mM | 3436 | s^-1 | 128 | s^-1 mM^-1 | Wild type | 10,544,015 |