Enzyme
stringlengths 4
59
| Organism
stringlengths 1
70
| Substrate
stringlengths 1
86
| km
float64 0
1.4M
⌀ | Unit_Km
stringclasses 65
values | Kcat
stringlengths 1
16
| Unit_Kcat
stringclasses 134
values | Kcat/Km
stringlengths 1
21
| Unit_Kcat/Km
stringclasses 335
values | Commentary[Temp]
stringclasses 165
values | Commentary[pH]
stringclasses 225
values | Commentary[Mutant]
stringlengths 1
93
| Commentary[Cosubstrate]
stringlengths 1
89
| pubmed id
float64 1.5M
35.5M
⌀ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
BglB | Pyrococcus horikoshii | pNP-Gal | 7.8 | mM | 571 | s^-1 | 75.8 | s^-1mM^-1 | 90°C | 4.75 | D206N | 11,900,558 |
|
BglB | Pyrococcus horikoshii | pNP-Man | 3.2 | mM | 45.0 | s^-1 | 14.7 | s^-1mM^-1 | 90°C | 4.75 | D206N | 11,900,558 |
|
BglB | Pyrococcus horikoshii | pNP-Glc | 30.4 | mM | 439 | s^-1 | 14.4 | s^-1mM^-1 | 90°C | 4.75 | Q77R/D206N | 11,900,558 |
|
BglB | Pyrococcus horikoshii | pNP-Gal | 36.1 | mM | 66.5 | s^-1 | 1.8 | s^-1mM^-1 | 90°C | 4.75 | Q77R/D206N | 11,900,558 |
|
BglB | Pyrococcus horikoshii | pNP-Man | 0.68 | mM | 6.8 | s^-1 | 9.9 | s^-1mM^-1 | 90°C | 4.75 | Q77R/D206N | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Glc | 0.19 | mM | 1140 | s^-1 | 7337 | s^-1mM^-1 | 90°C | 4.75-5.0 | Wild-type | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Gal | 5 | mM | 2827 | s^-1 | 561 | s^-1mM^-1 | 90°C | 4.75-5.0 | Wild-type | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Man | 1.3 | mM | 65.9 | s^-1 | 49.8 | s^-1mM^-1 | 90°C | 4.75-5.0 | Wild-type | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Glc | 13.8 | mM | 207 | s^-1 | 14.9 | s^-1mM^-1 | 90°C | 4.75-5.0 | R77Q | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Gal | 21.7 | mM | 69.2 | s^-1 | 3.2 | s^-1mM^-1 | 90°C | 4.75-5.0 | R77Q | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Man | 0.64 | mM | 1.22 | s^-1 | 1.9 | s^-1mM^-1 | 90°C | 4.75-5.0 | R77Q | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Man | 0.15 | mM | 0.85 | s^-1 | 5.7 | s^-1mM^-1 | 90°C | 4.75-5.0 | R77Q | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Glc | 4.6 | mM | 116 | s^-1 | 24.9 | s^-1mM^-1 | 90°C | 4.75-5.0 | N206D | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Gal | 15.7 | mM | 180 | s^-1 | 11.5 | s^-1mM^-1 | 90°C | 4.75-5.0 | N206D | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Man | 0.63 | mM | 1.97 | s^-1 | 3.1 | s^-1mM^-1 | 90°C | 4.75-5.0 | N206D | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Glc | 13.9 | mM | 172 | s^-1 | 12.3 | s^-1mM^-1 | 90°C | 4.75-5.0 | R77Q/N206D | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Gal | 22 | mM | 29.2 | s^-1 | 1.3 | s^-1mM^-1 | 90°C | 4.75-5.0 | R77Q/N206D | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Man | 1.5 | mM | 1.9 | s^-1 | 1.3 | s^-1mM^-1 | 90°C | 4.75-5.0 | R77Q/N206D | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Man | 0.1 | mM | 1.02 | s^-1 | 10.2 | s^-1mM^-1 | 90°C | 4.75-5.0 | R77Q/N206D | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Glc | 12 | mM | 309 | s^-1 | 26 | s^-1mM^-1 | 90°C | 4.75-5.0 | N206S | 11,900,558 |
|
CelB | Pyrococcus furiosus | pNP-Gal | 200 | mM | 1090 | s^-1 | 5.5 | s^-1mM^-1 | 90°C | 4.75-5.0 | N206S | 11,900,558 |
|
CCA-adding enzyme | Escherichia coli | Val-34C | 332 | µM | 0.73 | min^-1 | 2.2 × 10^3 | min^-1M^-1 | 37°C | Wild type | CTP | 11,926,813 |
|
CCA-adding enzyme | Escherichia coli | Val-34C | 144 | µM | 15.9 | min^-1 | 1.1 × 10^5 | min^-1M^-1 | 37°C | Wild type | ATP | 11,926,813 |
|
CCA-adding enzyme | Methanococcus jannaschii | Val-34C | 430 | µM | 1.2 | min^-1 | 2.8 × 10^3 | min^-1M^-1 | 70°C | Wild type | CTP | 11,926,813 |
|
CCA-adding enzyme | Methanococcus jannaschii | Val-34C | 287 | µM | 11.5 | min^-1 | 4.0 × 10^4 | min^-1M^-1 | 70°C | Wild type | ATP | 11,926,813 |
|
CCA-adding enzyme | Sulfolobus shibatae | Val-34C | 515 | µM | 0.16 | min^-1 | 3.1 × 10^2 | min^-1M^-1 | 70°C | Wild type | CTP | 11,926,813 |
|
CCA-adding enzyme | Sulfolobus shibatae | Val-34C | 500 | µM | 2.1 | min^-1 | 4.2 × 10^3 | min^-1M^-1 | 70°C | Wild type | ATP | 11,926,813 |
|
CCA-adding enzyme | Escherichia coli | Val-34C | 366 | µM | 0.07 | min^-1 | 2.0 × 10^2 | min^-1M^-1 | 37°C | Wild type | CTP | 11,926,813 |
|
CCA-adding enzyme | Escherichia coli | Val-34C | 263 | µM | 1.3 | min^-1 | 5 × 10^3 | min^-1M^-1 | 37°C | Wild type | ATP | 11,926,813 |
|
CCA-adding enzyme | Escherichia coli | Ala-75C | 145 | µM | 0.08 | min^-1 | 5.5 × 10^2 | min^-1M^-1 | 37°C | Wild type | CTP | 11,926,813 |
|
LM-M1 | Amycolatopsis mediterranei | Benzoate | 1,100 | µM | 0.11 | min^-1 | 100 | M^-1min^-1 | 30°C | 7.4 | Holo | ATP, NADPH | 11,955,082 |
LM-M1 | Amycolatopsis mediterranei | 3-Hydroxybenzoate | 1,100 | µM | 0.5 | min^-1 | 460 | M^-1min^-1 | 30°C | 7.4 | Holo | ATP, NADPH | 11,955,082 |
LM-M1 | Amycolatopsis mediterranei | 3-Amino-5-hydroxybenzoate | 10 | µM | 30°C | 7.4 | Holo | ATP, NADPH | 11,955,082 |
||||
LM-M1 | Amycolatopsis mediterranei | Benzoate | null | 140 | M^-1min^-1 | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||
LM-M1 | Amycolatopsis mediterranei | 3-Amino-5-hydroxybenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
LM-M1 | Amycolatopsis mediterranei | 3-Hydroxybenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
LM-M1 | Amycolatopsis mediterranei | 3,5-Dihydroxybenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
LM-M1 | Amycolatopsis mediterranei | 3,5-Dichlorobenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
LM-M1 | Amycolatopsis mediterranei | 3-Aminobenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
LM-M1 | Amycolatopsis mediterranei | 3,5-Dibromobenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
LM-M1 | Amycolatopsis mediterranei | 3-Chlorobenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
LM-M1 | Amycolatopsis mediterranei | 3-Bromobenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
LM-M1 | Amycolatopsis mediterranei | 2-Aminobenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
LM-M1 | Amycolatopsis mediterranei | 3-Methoxybenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
LM-M1 | Amycolatopsis mediterranei | 3-Fluorobenzoate | null | 30°C | 7.4 | Holo | ATP, NADPH, mmCoA | 11,955,082 |
|||||
TMG | Thermotoga maritima | Maltotriose | 20.64 | mM | 3.20 | s^-1 | 0.16 | s^-1mM^-1 | 85°C | 6.5 | 12,127,967 |
||
TMG | Thermotoga maritima | Maltotetraose | 11.84 | mM | 4.34 | s^-1 | 0.37 | s^-1mM^-1 | 85°C | 6.5 | 12,127,967 |
||
TMG | Thermotoga maritima | Maltopentaose | 9.95 | mM | 5.18 | s^-1 | 0.52 | s^-1mM^-1 | 85°C | 6.5 | 12,127,967 |
||
TMG | Thermotoga maritima | Maltohexaose | 7.77 | mM | 4.56 | s^-1 | 0.59 | s^-1mM^-1 | 85°C | 6.5 | 12,127,967 |
||
TMG | Thermotoga maritima | Maltoheptaose | 7.41 | mM | 4.09 | s^-1 | 0.55 | s^-1mM^-1 | 85°C | 6.5 | 12,127,967 |
||
TMG | Thermotoga maritima | pNPG5 | 2.26 | mM | 16.28 | s^-1 | 7.2 | s^-1mM^-1 | 85°C | 6.5 | 12,127,967 |
||
Est | Pyrobaculum calidifontis | p-Nitrophenyl propionate | 348 | µM | 1480 | s^-1 | 4.3 | s^-1µM^-1 | 70°C | 7.1 | 12,147,492 |
||
Est | Pyrobaculum calidifontis | p-Nitrophenyl butyrate | 164 | µM | 1690 | s^-1 | 10 | s^-1µM^-1 | 70°C | 7.1 | 12,147,492 |
||
Est | Pyrobaculum calidifontis | p-Nitrophenyl valerate | 71 | µM | 2070 | s^-1 | 29 | s^-1µM^-1 | 70°C | 7.1 | 12,147,492 |
||
Est | Pyrobaculum calidifontis | p-Nitrophenyl caproate | 44.4 | µM | 2620 | s^-1 | 59 | s^-1µM^-1 | 70°C | 7.1 | 12,147,492 |
||
Est | Pyrobaculum calidifontis | p-Nitrophenyl caprylate | 51 | µM | 1600 | s^-1 | 31 | s^-1µM^-1 | 70°C | 7.1 | 12,147,492 |
||
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | WT | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.058 | µM | 1.2 | s^-1 | 2.1 × 10^7 | M^-1s^-1 | 60°C | 8.0 | R40G | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.058 | µM | 1.2 | s^-1 | 2.1 × 10^7 | M^-1s^-1 | 60°C | 8.0 | R42G | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.083 | µM | 1.2 | s^-1 | 1.4 × 10^7 | M^-1s^-1 | 60°C | 8.0 | R40Q | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | R42Q | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.16 | µM | 0.76 | s^-1 | 4.8 × 10^6 | M^-1s^-1 | 60°C | 8.0 | R42E | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.22 | µM | 1.2 | s^-1 | 5.5 × 10^6 | M^-1s^-1 | 60°C | 8.0 | R40G/R42G | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.22 | µM | 1.2 | s^-1 | 5.5 × 10^6 | M^-1s^-1 | 60°C | 8.0 | R40Q/R42Q | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.87 | µM | 0.28 | s^-1 | 3.2 × 10^5 | M^-1s^-1 | 60°C | 8.0 | R40E/R42E | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | K51A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | R53A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.025 | µM | 1.2 | s^-1 | 4.8 × 10^7 | M^-1s^-1 | 60°C | 8.0 | K51E/R53E | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.17 | µM | 1.2 | s^-1 | 7.1 × 10^6 | M^-1s^-1 | 60°C | 8.0 | L47G | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | L47F | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | G44A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | G52A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.033 | µM | 1.2 | s^-1 | 3.6 × 10^7 | M^-1s^-1 | 60°C | 8.0 | K193A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.041 | µM | 1.2 | s^-1 | 2.9 × 10^7 | M^-1s^-1 | 60°C | 8.0 | R194A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.066 | µM | 1.2 | s^-1 | 1.8 × 10^7 | M^-1s^-1 | 60°C | 8.0 | K195A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.33 | µM | 0.76 | s^-1 | 2.3 × 10^6 | M^-1s^-1 | 60°C | 8.0 | K193A/R194A/K195A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.58 | µM | 0.48 | s^-1 | 8.3 × 10^5 | M^-1s^-1 | 60°C | 8.0 | K193E/R194E/K195E | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | K199A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | K243A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | K243E | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | K248A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | K249A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | K263A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | K263E | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | Y237A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 0.003 | s^-1 | 3.6 × 10^5 | M^-1s^-1 | 60°C | 8.0 | K87A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | R88A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | K89A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.041 | µM | 0.0065 | s^-1 | 1.6 × 10^5 | M^-1s^-1 | 60°C | 8.0 | K87A/R88A/K89A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | K93A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.1 | µM | 0.08 | s^-1 | 8.0 × 10^5 | M^-1s^-1 | 60°C | 8.0 | R94A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.0083 | µM | 1.2 | s^-1 | 1.4 × 10^8 | M^-1s^-1 | 60°C | 8.0 | R95A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.14 | µM | 0.0125 | s^-1 | 8.9 × 10^4 | M^-1s^-1 | 60°C | 8.0 | K93A/R94A/R95A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.025 | µM | 1.2 | s^-1 | 4.8 × 10^7 | M^-1s^-1 | 60°C | 8.0 | R118A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.025 | µM | 1.2 | s^-1 | 4.8 × 10^7 | M^-1s^-1 | 60°C | 8.0 | K119A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Flap substrate | 0.083 | µM | 1.2 | s^-1 | 1.4 × 10^7 | M^-1s^-1 | 60°C | 8.0 | R118A/K119A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Nick substrate | 0.5 | µM | 0.7 | s^-1 | 1.4 × 10^6 | M^-1s^-1 | 60°C | 8.0 | WT | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Nick substrate | 3 | µM | 0.7 | s^-1 | 2.3 × 10^5 | M^-1s^-1 | 60°C | 8.0 | R40G/R42G | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Nick substrate | 0.5 | µM | 0.0035 | s^-1 | 7.0 × 10^3 | M^-1s^-1 | 60°C | 8.0 | R94A | 12,147,694 |
|
FEN-1 | Pyrococcus horikoshii | Nick substrate | 8.5 | µM | 0.0063 | s^-1 | 7.4 × 10^2 | M^-1s^-1 | 60°C | 8.0 | R118A/K119A | 12,147,694 |