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from opentrons import protocol_api |
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metadata = { |
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'protocolName': 'My Protocol', |
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'author': 'Your Name', |
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'description': 'Description of the protocol', |
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'apiLevel': '2.12' |
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} |
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def run(protocol: protocol_api.ProtocolContext): |
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plate = protocol.load_labware('corning_96_wellplate_360ul_flat', '1') |
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tiprack = protocol.load_labware('opentrons_96_tiprack_300ul', '2') |
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pipette = protocol.load_instrument('p300_single_gen2', 'right', tip_racks=[tiprack]) |
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num_samples = 96 |
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sample_volume = 200 |
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reagent_volume = 50 |
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reagent_reservoir = protocol.load_labware('usascientific_12_reservoir_22ml', '3') |
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for well in plate.wells()[:num_samples]: |
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pipette.pick_up_tip() |
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pipette.aspirate(reagent_volume, reagent_reservoir.wells()[0]) |
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pipette.dispense(reagent_volume, well) |
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pipette.blow_out(well.top()) |
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pipette.drop_tip() |
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for well in plate.wells()[:num_samples]: |
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pipette.pick_up_tip() |
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pipette.mix(5, sample_volume, well) |
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pipette.drop_tip() |
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protocol.delay(minutes=30) |
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output_plate = protocol.load_labware('corning_96_wellplate_360ul_flat', '4') |
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for src_well, dest_well in zip(plate.wells()[:num_samples], output_plate.wells()[:num_samples]): |
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pipette.pick_up_tip() |
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pipette.aspirate(sample_volume, src_well) |
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pipette.dispense(sample_volume, dest_well) |
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pipette.blow_out(dest_well.top()) |
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pipette.drop_tip() |