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from opentrons import protocol_api
metadata = {
'protocolName': 'My Protocol',
'author': 'Your Name',
'description': 'Description of the protocol',
'apiLevel': '2.12'
}
def run(protocol: protocol_api.ProtocolContext):
# Labware
plate = protocol.load_labware('corning_96_wellplate_360ul_flat', '1')
tiprack = protocol.load_labware('opentrons_96_tiprack_300ul', '2')
pipette = protocol.load_instrument('p300_single_gen2', 'right', tip_racks=[tiprack])
# Parameters
num_samples = 96
sample_volume = 200
reagent_volume = 50
# Reagent reservoirs
reagent_reservoir = protocol.load_labware('usascientific_12_reservoir_22ml', '3')
# Transfer reagent to samples
for well in plate.wells()[:num_samples]:
pipette.pick_up_tip()
pipette.aspirate(reagent_volume, reagent_reservoir.wells()[0])
pipette.dispense(reagent_volume, well)
pipette.blow_out(well.top())
pipette.drop_tip()
# Mix samples
for well in plate.wells()[:num_samples]:
pipette.pick_up_tip()
pipette.mix(5, sample_volume, well)
pipette.drop_tip()
# Incubate samples
protocol.delay(minutes=30) # Incubate for 30 minutes
# Transfer samples to a new plate
output_plate = protocol.load_labware('corning_96_wellplate_360ul_flat', '4')
for src_well, dest_well in zip(plate.wells()[:num_samples], output_plate.wells()[:num_samples]):
pipette.pick_up_tip()
pipette.aspirate(sample_volume, src_well)
pipette.dispense(sample_volume, dest_well)
pipette.blow_out(dest_well.top())
pipette.drop_tip() |