Create test_protocol.py

test_protocol.py

@@ -0,0 +1,48 @@
+from opentrons import protocol_api
+
+metadata = {
+ 'protocolName': 'My Protocol',
+ 'author': 'Your Name',
+ 'description': 'Description of the protocol',
+ 'apiLevel': '2.12'
+}
+
+def run(protocol: protocol_api.ProtocolContext):
+ # Labware
+ plate = protocol.load_labware('corning_96_wellplate_360ul_flat', '1')
+ tiprack = protocol.load_labware('opentrons_96_tiprack_300ul', '2')
+ pipette = protocol.load_instrument('p300_single_gen2', 'right', tip_racks=[tiprack])
+
+ # Parameters
+ num_samples = 96
+ sample_volume = 200
+ reagent_volume = 50
+
+ # Reagent reservoirs
+ reagent_reservoir = protocol.load_labware('usascientific_12_reservoir_22ml', '3')
+
+ # Transfer reagent to samples
+ for well in plate.wells()[:num_samples]:
+ pipette.pick_up_tip()
+ pipette.aspirate(reagent_volume, reagent_reservoir.wells()[0])
+ pipette.dispense(reagent_volume, well)
+ pipette.blow_out(well.top())
+ pipette.drop_tip()
+
+ # Mix samples
+ for well in plate.wells()[:num_samples]:
+ pipette.pick_up_tip()
+ pipette.mix(5, sample_volume, well)
+ pipette.drop_tip()
+
+ # Incubate samples
+ protocol.delay(minutes=30) # Incubate for 30 minutes
+
+ # Transfer samples to a new plate
+ output_plate = protocol.load_labware('corning_96_wellplate_360ul_flat', '4')
+ for src_well, dest_well in zip(plate.wells()[:num_samples], output_plate.wells()[:num_samples]):
+ pipette.pick_up_tip()
+ pipette.aspirate(sample_volume, src_well)
+ pipette.dispense(sample_volume, dest_well)
+ pipette.blow_out(dest_well.top())
+ pipette.drop_tip()