test_protocol.py

from opentrons import protocol_api

metadata = {
 'protocolName': 'My Protocol',
 'author': 'Your Name',
 'description': 'Description of the protocol',
 'apiLevel': '2.12'
}

def run(protocol: protocol_api.ProtocolContext):
 # Labware
 plate = protocol.load_labware('corning_96_wellplate_360ul_flat', '1')
 tiprack = protocol.load_labware('opentrons_96_tiprack_300ul', '2')
 pipette = protocol.load_instrument('p300_single_gen2', 'right', tip_racks=[tiprack])

 # Parameters
 num_samples = 96
 sample_volume = 200
 reagent_volume = 50

 # Reagent reservoirs
 reagent_reservoir = protocol.load_labware('usascientific_12_reservoir_22ml', '3')

 # Transfer reagent to samples
 for well in plate.wells()[:num_samples]:
 pipette.pick_up_tip()
 pipette.aspirate(reagent_volume, reagent_reservoir.wells()[0])
 pipette.dispense(reagent_volume, well)
 pipette.blow_out(well.top())
 pipette.drop_tip()

 # Mix samples
 for well in plate.wells()[:num_samples]:
 pipette.pick_up_tip()
 pipette.mix(5, sample_volume, well)
 pipette.drop_tip()

 # Incubate samples
 protocol.delay(minutes=30) # Incubate for 30 minutes

 # Transfer samples to a new plate
 output_plate = protocol.load_labware('corning_96_wellplate_360ul_flat', '4')
 for src_well, dest_well in zip(plate.wells()[:num_samples], output_plate.wells()[:num_samples]):
 pipette.pick_up_tip()
 pipette.aspirate(sample_volume, src_well)
 pipette.dispense(sample_volume, dest_well)
 pipette.blow_out(dest_well.top())
 pipette.drop_tip()