{"tags": [0, 0, 3, 4, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Number", "of", "glucocorticoid", "receptors", "in", "lymphocytes", "and", "their", "sensitivity", "to", "hormone", "action", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "study", "demonstrated", "a", "decreased", "level", "of", "glucocorticoid", "receptors", "(", "GR", ")", "in", "peripheral", "blood", "lymphocytes", "from", "hypercholesterolemic", "subjects", ",", "and", "an", "elevated", "level", "in", "patients", "with", "acute", "myocardial", "infarction", "."]} {"tags": [0, 0, 5, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["In", "the", "lymphocytes", "with", "a", "high", "GR", "number", ",", "dexamethasone", "inhibited", "[", "3H", "]", "-thymidine", "and", "[", "3H", "]", "-acetate", "incorporation", "into", "DNA", "and", "cholesterol", ",", "respectively", ",", "in", "the", "same", "manner", "as", "in", "the", "control", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["On", "the", "other", "hand", ",", "a", "decreased", "GR", "number", "resulted", "in", "a", "less", "efficient", "dexamethasone", "inhibition", "of", "the", "incorporation", "of", "labeled", "compounds", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["These", "data", "showed", "that", "the", "sensitivity", "of", "lymphocytes", "to", "glucocorticoids", "changed", "only", "with", "a", "decrease", "of", "GR", "level", "."]} {"tags": [0, 3, 4, 4, 4, 4, 0, 5, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["[", "1", ",", "25-Dihydroxyvitamin", "D3", "receptors", "in", "lymphocytes", "and", "T-", "and", "B-lymphocyte", "count", "in", "patients", "with", "glomerulonephritis", "]"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Content", "of", "receptors", "to", "hormonal", "form", "of", "vitamin", "D3", ",", "1.25", "(", "OH", ")", "2D3", ",", "constituted", "27.3", "fmole/mg", "of", "protein", "in", "lymphocytes", "of", "peripheric", "blood", "of", "children", "with", "glomerulonephritis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "the", "patients", "concentration", "of", "total", "and", "ionized", "form", "of", "Ca2+", "was", "decreased", "down", "to", "2.04", "mmole/L", "and", "1.09", "mmole/L", ",", "respectively", ",", "while", "an", "increase", "in", "parathormone", "(", "PTH", ")", "by", "36", "%", "and", "a", "distinct", "decrease", "in", "25", "(", "OH", ")", "D", "concentration", "(", "lower", "than", "1.25", "ng/ml", ")", "was", "found", "in", "blood", ";", "content", "of", "cAMP", "was", "also", "decreased", "in", "lymphocytes", "by", "33", "%", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["At", "the", "same", "time", ",", "total", "content", "of", "T", "lymphocytes", "was", "decreased", "1.5-fold", "in", "peripheric", "blood", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Treatment", "with", "I-hydroxyvitamin", "D3", "(", "1-1.5", "mg", "daily", ",", "within", "4", "weeks", ")", "led", "to", "normalization", "of", "total", "and", "ionized", "form", "of", "Ca2+", "and", "of", "25", "(", "OH", ")", "D", ",", "but", "did", "not", "affect", "the", "PTH", "content", "in", "blood", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["Concentration", "of", "the", "receptors", "to", "1.25", "(", "OH", ")", "2D3", "was", "elevated", "up", "to", "39.7", "fmole/mg", "after", "I", "week", "of", "the", "treatment", ",", "whereas", "it", "was", "decreased", "to", "the", "initial", "level", "24.8", "fmole/mg", "within", "4", "weeks", ";", "simultaneous", "alteration", "in", "the", "cAMP", "content", "was", "observed", "in", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Treatment", "with", "1-", "(", "OH", ")", "D3", "normalized", "also", "the", "T", "lymphocytes", "content", "in", "peripheric", "blood", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "data", "obtained", "suggest", "that", "under", "conditions", "of", "glomerulonephritis", "only", "high", "content", "of", "receptors", "to", "1.25", "(", "OH", ")", "2D3", "in", "lymphocytes", "enabled", "to", "perform", "the", "cell", "response", "to", "the", "hormone", "effect", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tumor", "and", "serum", "beta-2-microglobulin", "expression", "in", "women", "with", "breast", "cancer", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["To", "investigate", "whether", "the", "tumor", "expression", "of", "beta-2-microglobulin", "(", "beta", "2-M", ")", "could", "serve", "as", "a", "marker", "of", "tumor", "biologic", "behavior", ",", "the", "authors", "studied", "specimens", "of", "breast", "carcinomas", "from", "60", "consecutive", "female", "patients", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Presence", "of", "beta", "2-M", "was", "analyzed", "by", "immunohistochemistry", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "significant", "correlations", "were", "found", "between", "tumor", "beta", "2-M", "expression", "and", "several", "histologic", "attributes", "such", "as", "type", ",", "histologic", "and", "nuclear", "grades", ",", "mitotic", "index", ",", "necrosis", ",", "vascular", "invasion", ",", "and", "lymphocytic", "infiltration", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Likewise", ",", "beta", "2-M", "was", "not", "associated", "with", "markers", "of", "disease", "extension", "such", "as", "TNM", ",", "(", "UICC", ",", "classification", "of", "malignant", "tumors", ")", "staging", "and", "axillary", "lymph", "node", "involvement", "or", "with", "estrogen", ",", "progesterone", ",", "and", "glucocorticoid", "receptor", "levels", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "there", "was", "a", "significantly", "positive", "association", "between", "tumor", "beta", "2-M", "expression", "and", "the", "degree", "of", "lymphocytic", "infiltration", "in", "the", "tumor", "tissue", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Beta", "2-M", "serum", "levels", "were", "determined", "by", "an", "enzyme-linked", "immunosorbent", "assay", "in", "samples", "from", "22", "of", "the", "above", "women", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "some", "of", "the", "highest", "values", "had", "been", "obtained", "in", "women", "with", "larger", "(", "T4", ")", "primary", "tumors", ",", "the", "authors", "failed", "to", "detect", "any", "statistical", "relationship", "between", "beta", "2-M", "expression", "in", "the", "tumor", "with", "serum", "levels", "or", "between", "serum", "beta", "2-M", "and", "the", "above", "histologic", ",", "laboratory", ",", "and", "clinical", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["[", "Preliminary", "observation", "of", "level", "free-form", "E", "receptor", "levels", "in", "serum", "of", "normal", "childbearing-aged", "and", "pregnant", "women", "]"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "137", "cases", "of", "childbearing-aged", "and", "pregnant", "women", ",", "free", "form", "E", "receptor", "levels", "(", "sE", ")", "in", "serum", "were", "measured", "by", "ELISA", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "level", "of", "sE", "was", "significantly", "decreased", "during", "the", "first", "trimester", ",", "slightly", "higher", "in", "the", "second", "trimester", ",", "and", "recovered", "to", "normal", "in", "the", "third", "trimester", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "level", "remained", "lower", "in", "29", "PIH", "women", "but", "appeared", "higher", "in", "overdue", "pregnancies", "as", "compared", "with", "the", "normal", "3rd", "trimester", "range", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["The", "results", "indicate", "that", "there", "is", "a", "relationship", "between", "a", "change", "in", "T", "cell", "function", "and", "pregnancy", "."]} {"tags": [3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0], "tokens": ["Kappa", "B-specific", "DNA", "binding", "proteins", ":", "role", "in", "the", "regulation", "of", "human", "interleukin-2", "gene", "expression", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Transcriptional", "activation", "of", "the", "human", "interleukin-2", "(", "IL-2", ")", "gene", ",", "like", "induction", "of", "the", "IL-2", "receptor", "alpha", "(", "IL-2R", "alpha", ")", "gene", "and", "the", "type", "1", "human", "immunodeficiency", "virus", "(", "HIV-1", ")", ",", "is", "shown", "to", "be", "modulated", "by", "a", "kappa", "B-like", "enhancer", "element", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Mutation", "of", "a", "kappa", "B", "core", "sequence", "identified", "in", "the", "IL-2", "promoter", "(", "-206", "to", "-195", ")", "partially", "inhibits", "both", "mitogen-", "and", "HTLV-I", "Tax-mediated", "activation", "of", "this", "transcription", "unit", "and", "blocks", "the", "specific", "binding", "of", "two", "inducible", "cellular", "factors", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 1, 2, 2, 0], "tokens": ["These", "kappa", "B-specific", "proteins", "(", "80", "to", "90", "and", "50", "to", "55", "kilodaltons", ")", "similarly", "interact", "with", "the", "functional", "kappa", "B", "enhancer", "present", "in", "the", "IL-2R", "alpha", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0], "tokens": ["These", "data", "suggest", "that", "these", "kappa", "B-specific", "proteins", "have", "a", "role", "in", "the", "coordinate", "regulation", "of", "this", "growth", "factor-growth", "factor", "receptor", "gene", "system", "that", "controls", "T", "cell", "proliferation", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 6, 0], "tokens": ["Novel", "region", "within", "the", "V", "kappa", "gene", "promoter", "is", "responsible", "for", "tissue", "and", "stage-specific", "expression", "of", "immunoglobulin", "genes", "in", "human", "lymphoid", "neoplasms", "."]} {"tags": [3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Immunoglobulin", "gene-specific", "transacting", "factors", "have", "been", "shown", "to", "play", "a", "role", "in", "lymphoid", "tissue-specific", "expression", "of", "immunoglobulin", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "role", "of", "these", "factors", "in", "B-cell", "differentiation", "and", "stage-specific", "expression", "of", "these", "genes", "is", ",", "however", ",", "not", "fully", "understood", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "used", "a", "model", "of", "human", "lymphoid", "neoplasia", "to", "address", "this", "question", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 1, 2, 2, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Different", "fragments", "of", "unrearranged", "human", "variable", "region", "of", "immunoglobulin", "kappa", "gene", "(", "V", "kappa", ")", "were", "used", "for", "cell-free", "in", "vitro", "transcription", "and", "DNA", "mobility", "shift", "assays", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["Previously", "described", "enhancement", "of", "in", "vitro", "transcription", "that", "was", "only", "seen", "with", "nuclear", "extracts", "derived", "from", "B-cell", "neoplasms", "corresponding", "to", "the", "late", "stages", "of", "B-cell", "differentiation", "was", "shown", "to", "be", "dependent", "on", "the", "actions", "of", "these", "factor", "(", "s", ")", "on", "the", "DNA", "region", "within", "the", "V", "kappa", "gene", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["This", "region", "is", "located", "within", "the", "920", "bp", "fragment", "located", "210", "bp", "upstream", "from", "the", "coding", "region", "and", "this", "fragment", "represents", "a", "possible", "novel", "DNA", "region", ",", "which", "plays", "a", "role", "in", "the", "stage-", "and", "tissue-specific", "expression", "of", "immunoglobulin", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["[", "Determination", "of", "the", "sensitivity", "to", "glucocorticoids", "in", "vitro", "]"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0], "tokens": ["A", "modified", "method", "for", "the", "determination", "of", "glucocorticoid", "receptors", "in", "human", "lymphocytes", "is", "suggested", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["The", "principal", "distinction", "of", "the", "method", "is", "standardization", "by", "the", "lymphocyte", "count", "in", "a", "sample", "(", "1", "mln", ")", "and", "the", "labeled", "hormone", "concentration", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "modification", "saves", "time", "and", "money", ",", "limits", "the", "range", "of", "the", "data", "variations", ",", "and", "makes", "use", "of", "a", "lesser", "volume", "of", "blood", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Examinations", "of", "70", "children", "aged", "4", "to", "15", "suffering", "from", "the", "nephrotic", "form", "of", "glomerulonephritis", "have", "made", "it", "possible", "to", "distinguish", "two", "groups", "of", "patients", ":", "with", "relatively", "high", "values", "of", "specific", "binding", "X", "=", "6820.1", "+/-", "530.0", "(", "n", "=", "30", ",", "p", "=", "0.95", ",", "t", "=", "2.04", ")", ",", "this", "corresponding", "to", "a", "clinical", "form", "of", "hormone", "-sensitive", "glomerulonephritis", ",", "and", "with", "relatively", "low", "values", "of", "specific", "binding", "X", "=", "1815.2", "+/-", "302.8", "(", "n", "=", "40", ",", "p", "=", "0.95", ",", "t", "=", "1.96", ")", ",", "that", "corresponds", "to", "hormone", "-resistant", "glomerulonephritis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Dynamic", "studies", "have", "not", "shown", "any", "statistically", "significant", "changes", "in", "the", "specific", "binding", "values", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "permit", "regarding", "the", "specific", "binding", "value", "as", "a", "prognostic", "criterion", "in", "the", "assessment", "of", "corticosteroid", "therapy", ";", "this", "allows", "a", "wide", "employment", "of", "the", "described", "method", "in", "practical", "nephrology", "."]} {"tags": [3, 4, 0, 7, 8, 8, 8, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0], "tokens": ["Octamer-binding", "proteins", "from", "B", "or", "HeLa", "cells", "stimulate", "transcription", "of", "the", "immunoglobulin", "heavy-chain", "promoter", "in", "vitro", "."]} {"tags": [0, 5, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "B-cell", "-type", "specificity", "of", "the", "immunoglobulin", "(", "Ig", ")", "heavy-chain", "and", "light-chain", "promoters", "is", "mediated", "by", "an", "octanucleotide", "(", "OCTA", ")", "element", ",", "ATGCAAAT", ",", "that", "is", "also", "a", "functional", "component", "of", "other", "RNA", "polymerase", "II", "promoters", ",", "such", "as", "snRNA", "and", "histone", "H2B", "promoters", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0], "tokens": ["Two", "nuclear", "proteins", "that", "bind", "specifically", "and", "with", "high", "affinity", "to", "the", "OCTA", "element", "have", "been", "identified", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 1, 2, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["NF-A1", "is", "present", "in", "a", "variety", "of", "cell", "types", ",", "whereas", "the", "presence", "of", "NF-A2", "is", "essentially", "confined", "to", "B", "cells", ",", "leading", "to", "the", "hypothesis", "that", "NF-A2", "activates", "cell-type-specific", "transcription", "of", "the", "Ig", "promoter", "and", "NF-A1", "mediates", "the", "other", "responses", "of", "the", "OCTA", "element", "."]} {"tags": [0, 0, 0, 7, 8, 0, 7, 0, 0, 0, 0, 0, 3, 0, 0, 0, 1, 2, 0], "tokens": ["Extracts", "of", "the", "B-cell", "line", ",", "BJA-B", ",", "contain", "high", "levels", "of", "NF-A2", "and", "specifically", "transcribe", "Ig", "promoters", "."]} {"tags": [0, 0, 0, 0, 0, 7, 8, 0, 0, 1, 2, 0, 0], "tokens": ["In", "contrast", ",", "extracts", "from", "HeLa", "cells", "transcribed", "the", "Ig", "promoter", "poorly", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Surprisingly", ",", "addition", "of", "either", "affinity-enriched", "NF-A2", "or", "NF-A1", "to", "either", "a", "HeLa", "extract", "or", "a", "partially", "purified", "reaction", "system", "specifically", "stimulates", "the", "Ig", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 1, 2, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["This", "suggests", "that", "the", "constitutive", "OCTA-binding", "factor", "NF-A1", "can", "activate", "transcription", "of", "the", "Ig", "promoter", "and", "that", "B-cell", "-specific", "transcription", "of", "this", "promoter", ",", "at", "least", "in", "vitro", ",", "is", "partially", "due", "to", "a", "quantitative", "difference", "in", "the", "amount", "of", "OCTA-binding", "protein", "."]} {"tags": [0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 1, 0], "tokens": ["Because", "NF-A1", "can", "stimulate", "Ig", "transcription", ",", "the", "inability", "of", "this", "factor", "to", "activate", "in", "vivo", "the", "Ig", "promoter", "to", "the", "same", "degree", "as", "the", "snRNA", "promoters", "probably", "reflects", "a", "difference", "in", "the", "context", "of", "the", "OCTA", "element", "in", "these", "two", "types", "of", "promoters", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0], "tokens": ["Identification", "of", "a", "putative", "regulator", "of", "early", "T", "cell", "activation", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 3, 0, 0], "tokens": ["Molecules", "involved", "in", "the", "antigen", "receptor-dependent", "regulation", "of", "early", "T", "cell", "activation", "genes", "were", "investigated", "with", "the", "use", "of", "functional", "sequences", "of", "the", "T", "cell", "activation-specific", "enhancer", "of", "interleukin-2", "(", "IL-2", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["One", "of", "these", "sequences", "forms", "a", "protein", "complex", ",", "NFAT-1", ",", "specifically", "with", "nuclear", "extracts", "of", "activated", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["This", "complex", "appeared", "10", "to", "25", "minutes", "before", "the", "activation", "of", "the", "IL-2", "gene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Studies", "with", "inhibitors", "of", "protein", "synthesis", "indicated", "that", "the", "time", "of", "synthesis", "of", "the", "activator", "of", "the", "IL-2", "gene", "in", "Jurkat", "T", "cells", "corresponds", "to", "the", "time", "of", "appearance", "of", "NFAT-1", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0], "tokens": ["NFAT-1", ",", "or", "a", "very", "similar", "protein", ",", "bound", "functional", "sequences", "of", "the", "long", "terminal", "repeat", "(", "LTR", ")", "of", "the", "human", "immunodeficiency", "virus", "type", "1", ";", "the", "LTR", "of", "this", "virus", "is", "known", "to", "be", "stimulated", "during", "early", "T", "cell", "activation", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["The", "binding", "site", "for", "this", "complex", "activated", "a", "linked", "promoter", "after", "transfection", "into", "antigen", "receptor-activated", "T", "cells", "but", "not", "other", "cell", "types", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["These", "characteristics", "suggest", "that", "NFAT-1", "transmits", "signals", "initiated", "at", "the", "T", "cell", "antigen", "receptor", "."]} {"tags": [0, 0, 3, 4, 4, 0, 7, 8, 8, 0], "tokens": ["Characterization", "of", "thyroid", "hormone", "receptors", "in", "human", "IM-9", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "putatively", "identified", "more", "than", "10", "years", "ago", ",", "thyroid", "hormone", "receptors", "in", "human", "tissues", "remain", "poorly", "characterized", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 7, 8, 8, 0, 7, 8, 0], "tokens": ["As", "a", "first", "step", "towards", "understanding", "the", "mechanism", "of", "thyroid", "hormone", "action", "in", "man", "we", "have", "characterized", "T3", "binding", "sites", "in", "nuclei", "of", "the", "human", "lymphoblastoid", "line", ",", "IM-9", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "whole", "cell", "experiments", "at", "37", "degrees", "C", ",", "nuclear", "binding", "of", "[", "125I", "]", "T3", "was", "saturable", "(", "Kd", "34", "+/-", "6", "pmol/l", ")", "and", "of", "finite", "capacity", "(", "approximately", "equal", "to", "350", "sites/cell", ")", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "binding", "sites", "were", "extracted", "from", "a", "nuclear", "pellet", "by", "treatment", "with", "0.4", "mol/l", "KCl", "and", "sonication", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Separation", "of", "bound", "from", "free", "[", "125I", "]", "T3", "in", "the", "extracts", "was", "achieved", "using", "the", "calcium", "phosphate", "matrix", ",", "hydroxyapatite", "at", "a", "concentration", "of", "0.3", "ml", "of", "a", "150", "g/l", "slurry", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Rectilinear", "Scatchard", "plots", "were", "obtained", "only", "when", "the", "hydroxyapatite", "was", "washed", "with", "a", "buffer", "containing", "0.5", "%", "Triton", "X-100", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Under", "these", "conditions", "T3", "binding", "sites", "in", "the", "nuclear", "extracts", "were", "present", "at", "a", "concentration", "of", "22.4", "+/-", "8.6", "fmol/mg", "protein", "and", "showed", "an", "affinity", "of", "(", "Kd", ",", "room", "temperature", ")", "140", "+/-", "10", "pmol/l", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "same", "assay", "system", "was", "used", "to", "determine", "the", "hierarchy", "of", "affinities", "for", "a", "range", "of", "natural", "and", "synthetic", "analogues", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Calling", "T3", "100", ",", "the", "order", "of", "potencies", "observed", "was", ":", "Triac", ",", "500", ";", "3", ",", "5-diiodo-3'-isopropylthyronine", ",", "89", ";", "T4", ",", "32", ";", "3", ",", "5-dimethyl-3'isopropylthyronine", "2", ";", "3", ",", "5-T2", ",", "0.7", ",", "rT3", ",", "0.4", ";", "3'5'-T2", ",", "less", "than", "0.01", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["These", "results", "suggest", "that", "the", "T3", "binding", "sites", "present", "in", "human", "IM-9", "lymphocyte", "nuclei", "and", "extracts", "thereof", "are", "thyroid", "hormone", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4], "tokens": ["These", "cells", "may", "be", "a", "useful", "tool", "to", "increase", "our", "understanding", "of", "human", "T3", "receptors"]} {"tags": [0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 1, 0, 0, 1, 2, 2, 2, 0], "tokens": ["Definition", "of", "T-cell", "specific", "DNA-binding", "factors", "that", "interact", "with", "a", "3'-silencer", "in", "the", "CD4+", "T-cell", "gene", "Rpt-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 5, 6, 6, 0, 0, 0, 5, 0, 5, 6, 6, 0], "tokens": ["Analysis", "of", "the", "region", "3", "'", "to", "the", "CD4+", "T-cell", "gene", "Rpt-1", "(", "encoding", "regulatory", "protein", "T-lymphocyte", "1", ")", "led", "to", "the", "definition", "of", "a", "silencer", "element", "that", "inhibits", "heterologous", "gene", "expression", "in", "certain", "CD4+", "T-cell", "lines", "but", "not", "in", "B-cell", "or", "non-lymphoid", "cell", "lines", "."]} {"tags": [0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["Functional", "silencer", "activity", "in", "vivo", "was", "associated", "with", "the", "presence", "of", "a", "specific", "silencer-DNA-protein", "complex", "in", "electrophoretic", "mobility", "shift", "assays", "with", "T-cell", "extracts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 1, 2, 0], "tokens": ["Formation", "of", "this", "complex", "was", "selectively", "inhibited", "by", "the", "region", "in", "HIV-1", "containing", "a", "silencer", "element", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["We", "discuss", "the", "possibility", "that", "DNA-binding", "factors", "may", "coregulate", "HIV-1", "and", "Rpt-1", "gene", "expression", "through", "a", "common", "transcriptional", "silencer", "element", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 5, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Congenital", "immunodeficiencies", "associated", "with", "absence", "of", "HLA", "class", "II", "antigens", "on", "lymphocytes", "result", "from", "distinct", "mutations", "in", "trans-acting", "factors", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Coordinate", "regulation", "of", "HLA", "class", "II", "gene", "expression", "during", "development", "and", "coinduction", "of", "class", "II", "genes", "by", "soluble", "factors", "suggests", "that", "common", "trans-acting", "factor", "(", "s", ")", "control", "expression", "of", "these", "genes", "."]} {"tags": [0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["In", "B-lymphoblastoid", "cell", "lines", "derived", "from", "two", "independent", "class", "II-deficient", "bare", "lymphocyte", "syndrome", "patients", ",", "we", "observed", "a", "drastic", "decrease", "in", "transcription", "rates", "of", "the", "class", "II", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["When", "these", "cell", "lines", "are", "fused", ",", "class", "II", "genes", "are", "reexpressed", ",", "indicating", "that", "immunodeficiencies", "in", "bare", "lymphocyte", "syndrome", "patients", "are", "the", "result", "of", "two", "distinct", "mutations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0], "tokens": ["Further", "studies", "show", "that", "genes", "governing", "the", "expression", "of", "class", "II", "antigens", "fall", "into", "at", "least", "three", "complementation", "groups", ";", "two", "of", "these", "were", "previously", "unidentified", "in", "mutant", "cell", "lines", "generated", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["In", "addition", ",", "we", "report", "the", "identification", "of", "two", "discrete", "complexes", ",", "NFX1.1", "and", "NFX1.2", ",", "that", "bind", "to", "the", "DRA", "X", "consensus", "element", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Though", "the", "mutation", "in", "at", "least", "one", "mutant", "line", "generated", "in", "vitro", "(", "RJ2.2.5", ")", "affects", "products", "functioning", "via", "interaction", "with", "the", "X", "box", ",", "clear", "alterations", "in", "either", "NFX1.1", "or", "NFX1.2", "are", "not", "found", "in", "any", "of", "the", "mutant", "cell", "lines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["In", "vivo", "responsiveness", "to", "glucocorticoid", "correlated", "with", "glucocorticoid", "receptor", "content", "in", "peripheral", "blood", "leukocytes", "in", "normal", "humans", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Dexamethasone", "loading", "tests", "(", "0.1", "mg", "dexamethasone/kg", ",", "iv", ")", "were", "performed", "in", "18", "normal", "males", "to", "evaluate", "the", "individual", "responsiveness", "to", "glucocorticoid", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "were", "inter-individual", "differences", "in", "increase", "in", "peripheral", "blood", "polymorphonuclear", "leukocyte", "count", ",", "decrease", "in", "peripheral", "blood", "lymphocyte", "count", ",", "and", "increase", "in", "plasma", "free", "fatty", "acids", "levels", "after", "dexamethasone", "injection", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "there", "was", "a", "significant", "correlation", "between", "the", "maximum", "increase", "in", "polymorphonuclear", "leukocytes", "and", "the", "maximum", "decrease", "in", "lymphocytes", "(", "r", "=", "0.7514", ",", "p", "less", "than", "0.0003", ")", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Simultaneous", "measurements", "of", "glucocorticoid", "receptor", "content", "by", "whole-cell", "assay", "revealed", "that", "glucocorticoid", "receptor", "content", "in", "polymorphonuclear", "leukocytes", "linearly", "correlated", "with", "that", "in", "the", "corresponding", "lymphocytes", "(", "r", "=", "0.9482", ",", "p", "less", "than", "0.0001", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 3, 4, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 3, 4, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "were", "also", "significant", "correlations", "between", "the", "maximum", "increase", "in", "polymorphonuclear", "leukocytes", "and", "glucocorticoid", "receptor", "content", "in", "polymorphonuclear", "leukocytes", "(", "r", "=", "0.7239", ",", "p", "less", "than", "0.0007", ")", ",", "and", "between", "the", "maximum", "decrease", "in", "lymphocytes", "and", "glucocorticoid", "receptor", "content", "in", "lymphocytes", "(", "r", "=", "0.7703", ",", "p", "less", "than", "0.0002", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["These", "results", "suggest", "that", "individual", "differences", "are", "preserved", "both", "in", "glucocorticoid", "responsiveness", "and", "in", "glucocorticoid", "receptor", "content", "in", "peripheral", "blood", "leukocytes", "in", "normal", "humans", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Estradiol", "receptors", "in", "the", "cytosol", "of", "peripheral", "blood", "mononuclear", "cells", "in", "hepatitis", "B", "virus", "carriers", "treated", "with", "interferon-alpha", "."]} {"tags": [3, 4, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Estradiol", "receptors", "in", "the", "cytosol", "of", "peripheral", "blood", "mononuclear", "cells", "and", "the", "effects", "of", "interferon-alpha", "(", "IFN-alpha", ")", "on", "estradiol", "receptors", "were", "studied", "in", "asymptomatic", "hepatitis", "B", "virus", "(", "HBV", ")", "carriers", ",", "patients", "with", "chronic", "hepatitis", "B", "and", "normal", "controls", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "level", "of", "estradiol", "receptors", "in", "the", "cytosol", "of", "mononuclear", "cells", "was", "significantly", "lower", "in", "asymptomatic", "HBV", "carriers", "and", "patients", "with", "chronic", "hepatitis", "B", ",", "compared", "to", "normal", "controls", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["This", "low", "level", "of", "cytosol", "estradiol", "receptors", "in", "patients", "with", "chronic", "hepatitis", "B", "was", "increased", "by", "the", "administration", "of", "IFN-alpha", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["In", "addition", ",", "when", "peripheral", "blood", "mononuclear", "cells", "from", "patients", "with", "chronic", "hepatitis", "B", "were", "incubated", "with", "IFN-alpha", "in", "vitro", ",", "the", "level", "of", "cytosol", "estradiol", "receptors", "also", "increased", "by", "increasing", "the", "concentration", "of", "IFN-alpha", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 0], "tokens": ["We", "previously", "reported", "that", "the", "response", "of", "mononuclear", "cells", "to", "estrogen", "is", "impaired", "in", "HBV", "carriers", ",", "and", "our", "present", "results", "suggested", "that", "this", "may", "be", "due", "to", "the", "low", "level", "of", "estradiol", "receptors", "in", "the", "cytosol", "of", "mononuclear", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Association", "of", "increased", "lytic", "effector", "cell", "function", "with", "high", "estrogen", "receptor", "levels", "in", "tumor-bearing", "patients", "with", "breast", "cancer", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tumor-bearing", "patients", "with", "breast", "cancer", "were", "assayed", "for", "their", "natural", "killer", "(", "NK", ")", "cell", "activity", "and", "for", "the", "function", "of", "activated", "cytotoxic", "T-cells", ",", "as", "assessed", "by", "lectin-dependent", "cellular", "cytotoxicity", "(", "LDCC", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tumor-bearing", "patients", "with", "breast", "cancer", "had", "a", "significant", "increase", "in", "NK", "activity", "and", "in", "LDCC", ",", "as", "compared", "with", "healthy", "control", "individuals", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0], "tokens": ["Although", "the", "enhanced", "NK", "cell", "activity", "and", "LDCC", "were", "closely", "associated", "with", "high", "levels", "(", "greater", "than", "31", "fmol/mg", ")", "of", "estrogen", "receptor", "(", "ER", ")", "content", "in", "the", "primary", "tumor", ",", "no", "other", "clinical", "or", "histologic", "correlation", "between", "the", "increase", "in", "either", "parameter", "of", "cytotoxic", "effector", "cell", "function", "could", "be", "found", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "ER", "levels", "greater", "than", "31", "fmol/mg", "might", "be", "associated", "with", "increased", "cytotoxic", "effector", "cell", "function", "in", "tumor-bearing", "patients", "with", "breast", "cancer", "."]} {"tags": [0, 0, 3, 4, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Properties", "of", "glucocorticoid", "receptors", "in", "Epstein-Barr", "virus-transformed", "lymphocytes", "from", "patients", "with", "familial", "cortisol", "resistance", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0, 7, 8, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["In", "a", "previous", "report", "of", "two", "patients", "with", "familial", "glucocorticoid", "resistance", "due", "to", "reduced", "numbers", "of", "glucocorticoid", "receptors", "(", "GR", ")", ",", "we", "have", "shown", "decreased", "numbers", "of", "GR", "in", "peripheral", "mononuclear", "cells", "and", "cultured", "fibroblasts", "but", "normal", "affinity", "of", "GR", "in", "both", "patients", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", ",", "peripheral", "lymphocytes", "from", "these", "patients", ",", "one", "patient", "'s", "son", "and", "daughter", ",", "and", "normal", "subjects", "were", "transformed", "with", "Epstein-Barr", "virus", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Reduced", "numbers", "and", "normal", "affinity", "of", "GR", "were", "found", "in", "the", "Epstein-Barr", "virus-transformed", "lymphocytes", "from", "both", "patients", "while", "the", "son", "and", "daughter", "had", "normal", "numbers", "and", "affinity", "of", "GR", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "thermal", "stability", "of", "GR", "and", "thermal", "activation", "of", "cytosolic", "receptors", "in", "both", "patients", "were", "found", "to", "be", "normal", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "the", "percentages", "of", "nuclear", "bound", "GR", "were", "similar", "in", "both", "patients", "and", "normal", "controls", ",", "the", "absolute", "amounts", "of", "nuclear", "bound", "GR", "of", "the", "patients", "were", "about", "one-half", "that", "of", "normal", "controls", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0], "tokens": ["These", "abnormal", "properties", "of", "GR", "(", "reduced", "numbers", "of", "GR", ")", "were", "preserved", "in", "the", "transformed", "cells", "from", "the", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 0], "tokens": ["Octamer", "transcription", "factors", "1", "and", "2", "each", "bind", "to", "two", "different", "functional", "elements", "in", "the", "immunoglobulin", "heavy-chain", "promoter", "."]} {"tags": [1, 2, 2, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Immunoglobulin", "heavy-chain", "genes", "contain", "two", "conserved", "sequence", "elements", "5", "'", "to", "the", "site", "of", "transcription", "initiation", ":", "the", "octamer", "ATGCAAAT", "and", "the", "heptamer", "CTCATGA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0], "tokens": ["Both", "of", "these", "elements", "are", "required", "for", "normal", "cell-specific", "promoter", "function", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["The", "present", "study", "demonstrates", "that", "both", "the", "ubiquitous", "and", "lymphoid-cell-specific", "octamer", "transcription", "factors", "(", "OTF-1", "and", "OTF-2", ",", "respectively", ")", "interact", "specifically", "with", "each", "of", "the", "two", "conserved", "sequence", "elements", ",", "forming", "either", "homo-", "or", "heterodimeric", "complexes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "was", "surprising", ",", "since", "the", "heptamer", "and", "octamer", "sequence", "motifs", "bear", "no", "obvious", "similarity", "to", "each", "other", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0], "tokens": ["Binding", "of", "either", "factor", "to", "the", "octamer", "element", "occurred", "independently", "."]} {"tags": [0, 0, 3, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "OTF", "interaction", "with", "the", "heptamer", "sequence", "appeared", "to", "require", "the", "presence", "of", "an", "intact", "octamer", "motif", "and", "occurred", "with", "a", "spacing", "of", "either", "2", "or", "14", "base", "pairs", "between", "the", "two", "elements", ",", "suggesting", "coordinate", "binding", "resulting", "from", "protein-protein", "interactions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "degeneracy", "in", "sequences", "recognized", "by", "the", "OTFs", "may", "be", "important", "in", "widening", "the", "range", "over", "which", "gene", "expression", "can", "be", "modulated", "and", "in", "establishing", "cell", "type", "specificity", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Identification", "of", "a", "novel", "lymphoid", "specific", "octamer", "binding", "protein", "(", "OTF-2B", ")", "by", "proteolytic", "clipping", "bandshift", "assay", "(", "PCBA", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 0], "tokens": ["The", "octamer", "sequence", "ATGCAAAT", "is", "found", "in", "the", "promoters", "of", "immunoglobulin", "(", "Ig", ")", "heavy", "and", "light", "chain", "genes", "and", "in", "the", "heavy", "chain", "enhancer", "and", "is", "a", "major", "determinant", "of", "the", "cell", "type", "specific", "expression", "of", "Ig", "genes", "in", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 1, 2, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0], "tokens": ["An", "apparent", "paradox", "is", "that", "the", "same", "sequence", "serves", "as", "an", "upstream", "promoter", "or", "enhancer", "element", "in", "a", "variety", "of", "housekeeping", "genes", "such", "as", "the", "histone", "H2B", "and", "U", "snRNA", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "differential", "usage", "of", "this", "regulatory", "sequence", "motif", "is", "thought", "to", "be", "mediated", "by", "different", "species", "of", "octamer", "binding", "proteins", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["One", "species", "of", "100", "kd", ",", "designated", "OTF-1", ",", "is", "present", "in", "all", "cell", "types", "and", "may", "exert", "its", "activating", "function", "only", "when", "it", "can", "interact", "with", "additional", "adjacent", "transcription", "factors", "."]} {"tags": [0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 3, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 1, 0, 0, 1, 2, 0, 0, 0, 0], "tokens": ["The", "lymphoid", "cell", "specific", "octamer", "binding", "protein", "of", "60", "kd", "(", "OTF-2A", ")", "specifically", "stimulates", "Ig", "promoters", "which", "consist", "essentially", "of", "a", "TATA-box", "and", "an", "octamer", "sequence", "upstream", "of", "it", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Here", "we", "present", "evidence", "for", "yet", "another", "B", "cell", "specific", "octamer", "binding", "protein", "of", "75", "kd", "(", "OTF-2B", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["From", "several", "findings", ",", "including", "the", "absence", "of", "OTF-2B", "(", "but", "not", "OTF-2A", ")", "from", "a", "lymphocyte", "line", "that", "can", "not", "respond", "to", "the", "IgH", "enhancer", ",", "we", "propose", "a", "role", "of", "the", "novel", "octamer", "factor", "in", "the", "long", "range", "activation", "by", "the", "IgH", "enhancer", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["We", "have", "used", "the", "proteolytic", "clipping", "bandshift", "assay", "(", "PCBA", ")", "technique", "to", "distinguish", "the", "three", "different", "forms", "found", "in", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["This", "analysis", "indicates", "that", "the", "75", "kd-species", "OTF-2B", "is", "closely", "related", "to", "the", "60", "kd", "species", "OTF-2A", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0], "tokens": ["Inhibition", "of", "interleukin", "2", "-induced", "proliferation", "of", "cloned", "murine", "T", "cells", "by", "glucocorticoids", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0], "tokens": ["Possible", "involvement", "of", "an", "inhibitory", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 0, 0, 5, 6, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 0, 0, 0, 0], "tokens": ["The", "ability", "of", "glucocorticoids", "to", "inhibit", "interleukin", "2", "(", "IL", "2", ")", "-induced", "T", "cell", "proliferation", "in", "two", "cytotoxic", "T", "cell", "(", "CTL", ")", "clones", "has", "been", "studied", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["A", "complete", "inhibition", "of", "DNA", "synthesis", "by", "dexamethasone", "(", "Dx", ")", "could", "be", "observed", "when", "IL", "2-depleted", "cultures", "of", "CTL", "were", "either", "incubated", "for", "6", "h", "with", "the", "hormone", "prior", "to", "the", "addition", "of", "IL", "2", "or", "treated", "simultaneously", "with", "Dx", "and", "a", "low", "concentration", "of", "IL", "2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "significant", "reduction", "in", "the", "number", "and", "affinity", "of", "IL", "2", "receptors", "was", "seen", "after", "6", "h", "incubation", "with", "Dx", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["The", "order", "of", "potency", "observed", "with", "the", "different", "steroids", "indicated", "that", "this", "inhibitory", "effect", "was", "mediated", "through", "binding", "to", "a", "specific", "glucocorticoid", "receptor", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "action", "of", "these", "hormones", "possibly", "involves", "the", "synthesis", "of", "an", "inhibitory", "protein", "(", "s", ")", ",", "since", "the", "presence", "of", "cycloheximide", "during", "the", "incubation", "with", "Dx", "prevented", "the", "inhibition", "of", "DNA", "synthesis", "."]} {"tags": [0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 3, 4, 0], "tokens": ["Furthermore", ",", "supernatant", "from", "Dx-treated", "CTL", "contained", "a", "nondialyzable", "factor", "which", "inhibited", "DNA", "synthesis", "and", "cell", "growth", "of", "CTL", "clones", "induced", "by", "IL", "2", "."]} {"tags": [0, 0, 3, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Blocking", "of", "IL", "2", "synthesis", "and", "IL", "2", "receptor", "formation", "have", "been", "proposed", "as", "one", "of", "the", "major", "mechanisms", "of", "glucocorticoid-induced", "immunosuppression", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 3, 4, 0, 0], "tokens": ["Our", "results", "indicate", "that", "these", "hormones", "may", "also", "affect", "T", "cell", "proliferation", "by", "inhibiting", "IL", "2", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0], "tokens": ["Identification", "and", "purification", "of", "a", "human", "immunoglobulin-enhancer-binding", "protein", "(", "NF-kappa", "B", ")", "that", "activates", "transcription", "from", "a", "human", "immunodeficiency", "virus", "type", "1", "promoter", "in", "vitro", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "enhancer-binding", "factor", "NF-kappa", "B", ",", "which", "is", "found", "only", "in", "cells", "that", "transcribe", "immunoglobulin", "light", "chain", "genes", ",", "has", "been", "purified", "from", "nuclear", "extracts", "of", "Namalwa", "cells", "(", "human", "Burkitt", "lymphoma", "cells", ")", "by", "sequence-specific", "DNA", "affinity", "chromatography", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["The", "purified", "NF-kappa", "B", "has", "been", "identified", "as", "a", "51-kDa", "polypeptide", "by", "UV-crosslinking", "analysis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0], "tokens": ["``", "Footprint", "''", "and", "methylation-interference", "analyses", "have", "shown", "that", "purified", "NF-kappa", "B", "has", "a", "binding", "activity", "specific", "for", "the", "kappa", "light", "chain", "enhancer", "sequence", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 1, 2, 2, 2], "tokens": ["The", "purified", "factor", "activated", "in", "vitro", "transcription", "of", "the", "human", "immunodeficiency", "virus", "type", "I", "promoter", "by", "binding", "to", "an", "upstream", "NF-kappa", "B-binding", "site"]} {"tags": [5, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymphocyte", "glucocorticoid", "receptor", "binding", "in", "depression", ":", "normal", "values", "following", "recovery", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "number", "of", "glucocorticoid", "receptor", "sites", "in", "lymphocytes", "and", "plasma", "cortisol", "concentrations", "were", "measured", "in", "20", "patients", "who", "had", "recovered", "from", "major", "depressive", "disorder", "and", "20", "healthy", "control", "subjects", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "number", "of", "glucocorticoid", "receptor", "sites", "in", "lymphocytes", "from", "the", "recovered", "depressed", "group", "was", "not", "significantly", "different", "from", "that", "of", "the", "control", "group", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "the", "mean", "plasma", "cortisol", "concentration", "in", "recovered", "depressives", "was", "higher", "than", "in", "control", "subjects", ",", "the", "difference", "only", "just", "reached", "significance", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "study", "shows", "that", "the", "reduction", "in", "glucocorticoid", "receptor", "numbers", "which", "occurs", "during", "acute", "depressive", "illness", "does", "not", "persist", "on", "recovery", "and", "is", ",", "therefore", ",", "state-dependent", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0], "tokens": ["Identification", "and", "purification", "of", "a", "human", "lymphoid-specific", "octamer-binding", "protein", "(", "OTF-2", ")", "that", "activates", "transcription", "of", "an", "immunoglobulin", "promoter", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["The", "octamer", "sequence", "5'-ATGCAAAT", ",", "in", "either", "orientation", ",", "serves", "as", "an", "upstream", "element", "in", "a", "variety", "of", "promoters", "and", "also", "occurs", "as", "a", "modular", "enhancer", "element", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "is", "of", "particular", "interest", "in", "immunoglobulin", "genes", "since", "it", "is", "found", "in", "the", "upstream", "regions", "of", "all", "heavy", "and", "light", "chain", "promoters", "and", "in", "the", "heavy", "chain", "enhancer", ",", "both", "of", "which", "are", "known", "to", "be", "necessary", "for", "cell-specific", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0], "tokens": ["We", "report", "here", "the", "chromatographic", "separation", "of", "ubiquitous", "and", "B", "cell-specific", "octamer-binding", "proteins", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "B", "cell", "factor", "was", "purified", "to", "homogeneity", "using", "affinity", "chromatography", "and", "consists", "of", "three", "peptides", "of", "62", ",", "61", ",", "and", "58.5", "+/-", "1.5", "kd", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Each", "of", "the", "polypeptides", "was", "renatured", "after", "SDS-PAGE", "and", "shown", "to", "bind", "to", "the", "octamer", "sequence", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "specific", "DNA", "binding", "activity", "of", "the", "pure", "B", "cell-specific", "factor", "was", "indistinguishable", "from", "that", "of", "the", "affinity-purified", "ubiquitous", "factor", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0], "tokens": ["This", "B", "cell-specific", "octamer-binding", "factor", ",", "in", "pure", "form", ",", "activated", "transcription", "from", "a", "kappa", "light", "chain", "promoter", "in", "vitro", ",", "thus", "demonstrating", "that", "it", "is", "indeed", "a", "B", "cell-specific", "transcription", "factor", "for", "this", "gene", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["In", "addition", "to", "the", "ubiquitous", "and", "B", "cell-specific", "octamer-binding", "factors", ",", "we", "identified", "several", "additional", "proteins", ",", "one", "of", "which", "is", "B", "cell", "-specific", ",", "that", "interact", "with", "the", "kappa", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Decreased", "deoxyribonucleic", "acid", "binding", "of", "glucocorticoid-receptor", "complex", "in", "cultured", "skin", "fibroblasts", "from", "a", "patient", "with", "the", "glucocorticoid", "resistance", "syndrome", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "patient", "with", "the", "syndrome", "of", "glucocorticoid", "resistance", "was", "studied", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "27-yr-old", "woman", "initially", "was", "diagnosed", "as", "having", "Cushing", "'s", "disease", ",", "based", "on", "the", "findings", "of", "high", "plasma", "ACTH", "and", "serum", "cortisol", "levels", ",", "increased", "urinary", "cortisol", "secretion", ",", "resistance", "to", "adrenal", "suppression", "with", "dexamethasone", ",", "and", "bilateral", "adrenal", "hyperplasia", "by", "computed", "tomography", "and", "scintigraphy", "of", "the", "adrenal", "glands", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "she", "had", "no", "signs", "or", "symptoms", "of", "Cushing", "'s", "syndrome", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["During", "a", "5-yr", "follow-up", ",", "no", "clinical", "abnormalities", "developed", ",", "although", "hypercortisolism", "persisted", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["End-organ", "resistance", "to", "cortisol", "was", "suspected", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 5, 6, 6, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "explain", "the", "end-organ", "resistance", "to", "cortisol", ",", "the", "glucocorticoid", "receptors", "(", "GR", ")", "in", "peripheral", "mononuclear", "leukocytes", "and", "cultured", "skin", "fibroblasts", "from", "a", "forearm", "skin", "biopsy", "were", "characterized", "and", "compared", "with", "the", "results", "of", "similar", "studies", "in", "normal", "subjects", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "patient", "'s", "GR", "in", "whole", "cell", "assays", "had", "an", "increased", "dissociation", "constant", "(", "Kd", ")", "."]} {"tags": [0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "the", "cytosol", "of", "cultured", "skin", "fibroblasts", "from", "the", "patient", ",", "there", "was", "also", "decreased", "binding", "capacity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "thermal", "stability", "and", "the", "sedimentation", "coefficient", "in", "a", "sucrose", "density", "gradient", "of", "the", "receptors", "in", "the", "cytosol", "of", "cultured", "skin", "fibroblasts", "from", "the", "patient", "and", "normal", "subjects", "were", "similar", "."]} {"tags": [7, 8, 8, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0], "tokens": ["GR", "complex", "activation", ",", "analyzed", "by", "DEAE-cellulose", "chromatography", ",", "was", "decreased", "in", "the", "patient", "."]} {"tags": [1, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["DNA", "binding", "of", "the", "GR", "complex", "after", "temperature-induced", "activation", "was", "lower", "in", "the", "patient", "than", "in", "normal", "subjects", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nuclear", "translocation", "of", "GR", "complexes", "from", "the", "patient", "was", "also", "slightly", "decreased", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 1, 0], "tokens": ["These", "results", "suggest", "that", "the", "patient", "'s", "glucocorticoid", "resistance", "was", "due", "to", "a", "decrease", "in", "the", "affinity", "of", "the", "receptor", "for", "glucocorticoids", "and", "a", "decrease", "in", "the", "binding", "of", "the", "GR", "complex", "to", "DNA", "."]} {"tags": [3, 4, 4, 0], "tokens": ["Granulocyte-macrophage", "colony-stimulating", "factor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Sensitive", "and", "receptor-mediated", "regulation", "by", "1", ",", "25-dihydroxyvitamin", "D3", "in", "normal", "human", "peripheral", "blood", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 3, 4, 4, 3, 4, 4, 0, 3, 0, 0], "tokens": ["We", "show", "that", "1", ",", "25-dihydroxyvitamin", "D3", "(", "1", ",", "25", "[", "OH", "]", "2D3", ")", ",", "the", "most", "hormonally", "active", "metabolite", "of", "vitamin", "D3", ",", "modulates", "sensitively", "and", "specifically", "both", "the", "protein", "and", "messenger", "RNA", "accumulation", "of", "the", "multilineage", "growth", "factor", "granulocyte-macrophage", "colony-stimulating", "factor", "(", "GM-CSF", ")", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 8, 8, 8, 8, 8, 8, 8, 0, 0, 0, 0], "tokens": ["The", "regulation", "of", "GM-CSF", "expression", "is", "seen", "in", "both", "normal", "human", "mitogen-activated", "T", "lymphocytes", "and", "T", "lymphocytes", "from", "a", "line", "(", "S-LB1", ")", "transformed", "with", "human", "T", "cell", "lymphotropic", "virus", "1", "(", "HTLV-1", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "cells", "from", "a", "HTLV-1", "transformed", "T", "lymphocyte", "line", "(", "Ab-VDR", ")", "established", "from", "a", "patient", "with", "vitamin", "D-resistant", "rickets", "type", "II", "with", "undetectable", "1", ",", "25", "(", "OH", ")", "2D3", "cellular", "receptors", "are", "resistant", "to", "the", "action", "of", "1", ",", "25", "(", "OH", ")", "2D3", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0], "tokens": ["Inhibition", "of", "GM-CSF", "expression", "by", "1", ",", "25", "(", "OH", ")", "2D3", "can", "occur", "independently", "of", "interleukin", "2", "regulation", "and", "is", "probably", "mediated", "through", "cellular", "1", ",", "25", "(", "OH", ")", "2D3", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "conclude", "that", "1", ",", "25", "(", "OH", ")", "2D3", "may", "be", "important", "in", "the", "physiology", "of", "hematopoiesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Altered", "interaction", "between", "triiodothyronine", "and", "its", "nuclear", "receptors", "in", "absence", "of", "cortisol", ":", "a", "proposed", "mechanism", "for", "increased", "thyrotropin", "secretion", "in", "corticosteroid", "deficiency", "states", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thyroid", "hormones", "occasionally", "appear", "less", "effective", "when", "administered", "alone", "to", "patients", "with", "panhypopituitarism", ",", "and", "manifestations", "suggestive", "of", "hypothyroidism", "have", "been", "reported", "in", "patients", "suffering", "from", "untreated", "Addison", "'s", "disease", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "the", "latter", "condition", ",", "thyrotropin", "secretion", "is", "increased", ":", "this", "occurs", "already", "after", "as", "little", "as", "2", "days", "of", "temporary", "withdrawal", "of", "therapy", "with", "substitution", "doses", "of", "corticosteroids", "while", "circulating", "levels", "of", "thyroid", "hormones", "remain", "within", "normal", "limits", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", ",", "a", "possible", "role", "of", "cortisol", "in", "interaction", "between", "triiodothyronine", "and", "its", "nuclear", "receptors", "was", "examined", "at", "the", "level", "of", "circulating", "lymphocytes", "obtained", "from", "patients", "with", "primary", "or", "secondary", "adrenocortical", "failure", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "affinity", "of", "these", "receptors", "was", "found", "to", "be", "decreased", ",", "by", "more", "than", "50", "%", "on", "average", ",", "in", "the", "absence", "of", "cortisol", "treatments", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "change", "was", "promptly", "corrected", "upon", "resumption", "of", "therapy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "number", "of", "binding", "sites", "was", "not", "significantly", "modified", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "influence", "of", "cortisol", "on", "thyroid", "hormone", "receptors", "discussed", "here", "might", "account", "for", "the", "clinical", "observations", "mentioned", "above", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 0], "tokens": ["Inhibition", "by", "cortisol", "of", "human", "natural", "killer", "(", "NK", ")", "cell", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0], "tokens": ["The", "effects", "of", "cortisol", "on", "the", "natural", "killer", "(", "NK", ")", "activity", "of", "human", "peripheral", "blood", "mononuclear", "(", "PBM", ")", "cells", "were", "studied", "in", "vitro", "using", "a", "direct", "4-h", "51Cr-release", "assay", "and", "K", "562", "cell", "line", "as", "a", "target", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0], "tokens": ["Preincubation", "for", "20", "h", "of", "PBM", "cells", "drawn", "from", "healthy", "donors", "with", "1", "X", "10", "(", "-8", ")", "to", "1", "X", "10", "(", "-5", ")", "M", "cortisol", "resulted", "in", "a", "significant", "decrease", "of", "NK", "cell", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "magnitude", "of", "the", "suppression", "was", "directly", "related", "to", "the", "steroid", "concentration", "and", "inversely", "related", "to", "the", "number", "of", "effector", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cortisol", "was", "able", "to", "minimize", "the", "enhancement", "of", "NK", "cytotoxicity", "obtainable", "in", "the", "presence", "of", "immune", "interferon", "(", "IFN-gamma", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "significantly", "higher", "suppression", "was", "achieved", "after", "sequential", "exposure", "of", "PBM", "cells", "to", "cortisol", "and", "equimolar", "levels", "of", "prostaglandin", "E2", "(", "PgE2", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["The", "concomitant", "incubation", "with", "theophylline", "and", "isobutyl-methylxanthine", "failed", "to", "enhance", "the", "cortisol-induced", "suppression", ",", "whereas", "PgE2-dependent", "inhibition", "significantly", "increased", "after", "exposure", "of", "PBM", "cells", "to", "methyl-xanthines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "inhibitory", "effect", "of", "cortisol", "was", "partially", "or", "totally", "prevented", "by", "the", "concomitant", "incubation", "with", "equimolar", "amounts", "of", "11-deoxycortisol", "and", "RU", "486", "but", "not", "of", "progesterone", "."]} {"tags": [0, 0, 5, 6, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Treatment", "of", "NK", "effectors", "with", "a", "monoclonal", "anti-human", "corticosteroid-binding", "globulin", "(", "CBG", ")", "antibody", "produced", "an", "enhancement", "of", "the", "spontaneous", "NK", "activity", "and", "a", "partial", "suppression", "of", "cortisol-mediated", "effects", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "results", "suggest", "that", "endogenous", "glucocorticoids", "play", "a", "role", "in", "the", "regulation", "of", "NK", "cell-mediated", "cytotoxicity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0], "tokens": ["Since", "the", "effect", "of", "cortisol", "was", "additive", "to", "that", "of", "PgE2", "and", "was", "not", "changed", "by", "phosphodiesterase", "inhibitors", ",", "it", "is", "conceivable", "that", "the", "hormone", "acts", "at", "a", "level", "different", "from", "the", "adenylate", "cyclase", "-", "phosphodiesterase", "system", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0], "tokens": ["Data", "obtained", "with", "the", "use", "of", "antiglucocorticoids", "and", "the", "anti-CBG", "antibody", "are", "compatible", "with", "a", "role", "both", "of", "high-affinity", "glucocorticoid", "receptors", "and", "of", "CBG", "in", "mediating", "cortisol", "action", "on", "the", "human", "NK", "cell", "activity", "."]} {"tags": [0, 0, 3, 4, 4, 0, 1, 2, 2, 2, 2, 0], "tokens": ["Interaction", "of", "cell-type-specific", "nuclear", "proteins", "with", "immunoglobulin", "VH", "promoter", "region", "sequences", "."]} {"tags": [0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["All", "human", "and", "murine", "immunoglobulin", "heavy", "chain", "variable", "region", "(", "VH", ")", "genes", "contain", "the", "sequence", "ATGCAAAT", "approximately", "70", "nucleotides", "5", "'", "from", "the", "site", "of", "transcription", "initiation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0], "tokens": ["This", "octanucleotide", ",", "in", "reverse", "orientation", ",", "is", "also", "found", "in", "all", "light", "chain", "variable", "region", "(", "VL", ")", "genes", ",", "and", "in", "the", "immunoglobulin", "heavy", "chain", "transcriptional", "enhancer", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Transfection", "studies", "have", "established", "that", "this", "octamer", "is", "involved", "in", "the", "lymphoid-specific", "transcription", "of", "immunoglobulin", "genes", "."]} {"tags": [1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0], "tokens": ["Octamer-containing", "fragments", "have", "been", "reported", "to", "bind", "a", "factor", "present", "in", "nuclear", "extracts", "of", "human", "cell", "lines", ";", "however", ",", "identical", "binding", "activity", "was", "detected", "in", "both", "B", "lymphoid", "and", "non-lymphoid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Here", "we", "establish", "that", "nuclear", "extracts", "from", "distinct", "cell", "types", "differ", "in", "their", "ability", "to", "interact", "with", "octamer-containing", "fragments", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["We", "have", "also", "detected", "a", "DNA-protein", "interaction", "that", "may", "be", "involved", "in", "the", "cell-type", "specificity", "of", "immunoglobulin", "expression", ",", "and", "we", "have", "determined", "that", "a", "sequence", "upstream", "of", "the", "octamer", "participates", "in", "an", "interaction", "with", "a", "nuclear", "protein", "(", "s", ")", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 0, 0, 5, 6, 6, 0, 3, 4, 4, 0], "tokens": ["Preferential", "transcription", "of", "HTLV-I", "LTR", "in", "cell-free", "extracts", "of", "human", "T", "cells", "producing", "HTLV-I", "viral", "proteins", "."]} {"tags": [0, 1, 0, 0, 1, 2, 2, 2, 2, 0, 0, 1, 2, 2, 0, 0, 1, 2, 2, 2, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 7, 0, 7, 8, 8, 8, 8, 8, 0, 0, 7, 0, 7, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 7, 0, 7, 8, 8, 8, 8, 0, 3, 4, 0, 0], "tokens": ["The", "promoters", "of", "the", "adenovirus", "2", "major", "late", "gene", ",", "the", "mouse", "beta-globin", "gene", ",", "the", "mouse", "immunoglobulin", "VH", "gene", "and", "the", "LTR", "of", "the", "human", "T-lymphotropic", "retrovirus", "type", "I", "were", "tested", "for", "their", "transcription", "activities", "in", "cell-free", "extracts", "of", "four", "cell", "lines", ";", "HeLa", ",", "CESS", "(", "Epstein-Barr", "virus-transformed", "human", "B", "cell", "line", ")", ",", "MT-1", "(", "HTLV-I-infected", "human", "T", "cell", "line", "without", "viral", "protein", "synthesis", ")", ",", "and", "MT-2", "(", "HTLV-I-infected", "human", "T", "cell", "line", "producing", "viral", "proteins", ")", "."]} {"tags": [1, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["LTR", "was", "preferentially", "transcribed", "in", "the", "extracts", "of", "MT-2", "although", "the", "other", "three", "genes", "were", "transcribed", "with", "relatively", "constant", "efficiencies", "in", "different", "extracts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["The", "results", "agree", "well", "with", "the", "previous", "in", "vivo", "studies", "on", "the", "promoter", "activity", "of", "HTLV-I", "LTR", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mixing", "of", "HeLa", "and", "MT-2", "extracts", "revealed", "the", "presence", "of", "a", "LTR-specific", "stimulating", "activity", "in", "MT-2", "extracts", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 2, 0], "tokens": ["A", "nuclear", "factor", "that", "binds", "to", "a", "conserved", "sequence", "motif", "in", "transcriptional", "control", "elements", "of", "immunoglobulin", "genes", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 5, 6, 6, 6, 0], "tokens": ["Trans-acting", "factors", "that", "mediate", "B-cell", "specific", "transcription", "of", "immunoglobulin", "genes", "have", "been", "postulated", "based", "on", "an", "analysis", "of", "the", "expression", "of", "exogenously", "introduced", "immunoglobulin", "gene", "recombinants", "in", "lymphoid", "and", "non-lymphoid", "cells", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0], "tokens": ["Two", "B-cell-specific", ",", "cis-acting", "transcriptional", "regulatory", "elements", "have", "been", "identified", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["One", "element", "is", "located", "in", "the", "intron", "between", "the", "variable", "(", "V", ")", "and", "constant", "(", "C", ")", "regions", "of", "both", "heavy", "and", "kappa", "light-chain", "genes", "and", "acts", "as", "a", "transcriptional", "enhancer", "."]} {"tags": [0, 0, 1, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0], "tokens": ["The", "second", "element", "is", "found", "upstream", "of", "both", "heavy", "and", "kappa", "light-chain", "gene", "promoters", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["This", "element", "directs", "lymphoid-specific", "transcription", "even", "in", "the", "presence", "of", "viral", "enhancers", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "sought", "nuclear", "factors", "that", "might", "bind", "specifically", "to", "these", "two", "regulatory", "elements", "by", "application", "of", "a", "modified", "gel", "electrophoresis", "DNA", "binding", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["We", "report", "here", "the", "identification", "of", "a", "human", "B-cell", "nuclear", "factor", "(", "IgNF-A", ")", "that", "binds", "to", "DNA", "sequences", "in", "the", "upstream", "regions", "of", "both", "the", "mouse", "heavy", "and", "kappa", "light-chain", "gene", "promoters", "and", "also", "to", "the", "mouse", "heavy-chain", "gene", "enhancer", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["This", "sequence-specific", "binding", "is", "probably", "mediated", "by", "a", "highly", "conserved", "sequence", "motif", ",", "ATTTGCAT", ",", "present", "in", "all", "three", "transcriptional", "elements", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Interestingly", ",", "a", "factor", "showing", "similar", "binding", "specificity", "to", "IgNF-A", "is", "also", "present", "in", "human", "HeLa", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "new", "world", "primates", "as", "animal", "models", "of", "glucocorticoid", "resistance", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Many", "New", "World", "primate", "species", "have", "greatly", "increased", "plasma", "cortisol", "concentrations", ",", "decreased", "plasma", "cortisol", "binding", "globulin", "capacity", "and", "affinity", ",", "marked", "resistance", "of", "the", "hypothalamic-pituitary-adrenal", "axis", "to", "suppression", "by", "dexamethasone", ",", "and", "no", "biological", "evidence", "of", "glucocorticoid", "excess", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "primates", "also", "have", "high", "levels", "of", "circulating", "progesterone", ",", "estrogen", ",", "mineralocorticoid", ",", "androgen", "and", "vitamin", "D", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 7, 8, 8, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["The", "glucocorticoid", "target", "tissues", "that", "have", "been", "examined", "(", "circulating", "mononuclear", "lymphocytes", "and", "cultured", "skin", "fibroblasts", ")", "have", "normal", "concentrations", "of", "glucocorticoid", "receptors", "with", "decreased", "affinity", "for", "dexamethasone", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transformation", "of", "B-lymphocytes", "with", "the", "Epstein-Barr", "virus", "leads", "to", "glucocorticoid", "receptor", "induction", "that", "is", "less", "than", "that", "observed", "with", "cells", "from", "Old", "World", "primates", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "receptor", "in", "these", "cells", "has", "a", "low", "affinity", "for", "dexamethasone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "low", "affinity", "leads", "to", "an", "increased", "loss", "of", "specific", "bound", "ligand", "during", "thermal", "activation", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Meroreceptor", "generation", "is", "normal", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "molecular", "weight", "of", "the", "receptor", ",", "determined", "by", "SDS-PAGE", ",", "is", "similar", "to", "that", "of", "Old", "World", "primates", "(", "approximately", "92", ",", "000", ")", "and", "the", "activation", "pattern", "per", "se", ",", "examined", "in", "vitro", "by", "heating", "cytosol", "and", "performing", "phosphocellulose", "chromatography", ",", "appears", "similar", "to", "that", "of", "human", "controls", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "ratios", "of", "nuclear", "to", "cytosolic", "hormone-receptor-complexes", "and", "of", "cytosolic", "activated", "to", "unactivated", "receptor", "complexes", "in", "intact", "cells", "are", "similar", "to", "Old", "World", "primates", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Results", "from", "mixing", "studies", "do", "not", "support", "the", "hypothesis", "that", "a", "binding", "inhibitor", "(", "s", ")", "or", "a", "deficient", "cytosolic", "positive", "modifier", "(", "s", ")", "of", "binding", "underlies", "the", "findings", "in", "these", "primates", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "New", "World", "primates", ",", "unlike", "men", "with", "the", "syndrome", "of", "primary", "cortisol", "resistance", ",", "have", "compensated", "for", "their", "condition", "with", "intra-adrenal", "and", "mineralocorticoid", "receptor", "adaptations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "unlike", "Old", "World", "primates", ",", "cortisol", "in", "New", "World", "primates", "has", "only", "weak", "sodium-retaining", "potency", "because", "the", "aldosterone", "receptor", "has", "a", "low", "affinity", "for", "cortisol", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "common", "element", "that", "would", "explain", "the", "apparent", "resistance", "to", "six", "steroid", "hormones", "in", "New", "World", "primates"]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Acetylation", "and", "modulation", "of", "erythroid", "Kruppel-like", "factor", "(", "EKLF", ")", "activity", "by", "interaction", "with", "histone", "acetyltransferases", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Erythroid", "Kruppel-like", "factor", "(", "EKLF", ")", "is", "a", "red", "cell-specific", "transcriptional", "activator", "that", "is", "crucial", "for", "consolidating", "the", "switch", "to", "high", "levels", "of", "adult", "beta-globin", "expression", "during", "erythroid", "ontogeny", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["EKLF", "is", "required", "for", "integrity", "of", "the", "chromatin", "structure", "at", "the", "beta-like", "globin", "locus", ",", "and", "it", "interacts", "with", "a", "positive-acting", "factor", "in", "vivo", "."]} {"tags": [0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0], "tokens": ["We", "find", "that", "EKLF", "is", "an", "acetylated", "transcription", "factor", ",", "and", "that", "it", "interacts", "in", "vivo", "with", "CBP", ",", "p300", ",", "and", "P/CAF", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "its", "interactions", "with", "these", "histone", "acetyltransferases", "are", "not", "equivalent", ",", "as", "CBP", "and", "p300", ",", "but", "not", "P/CAF", ",", "utilize", "EKLF", "as", "a", "substrate", "for", "in", "vitro", "acetylation", "within", "its", "trans-activation", "region", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 0], "tokens": ["The", "functional", "effects", "of", "these", "interactions", "are", "that", "CBP", "and", "p300", ",", "but", "not", "P/CAF", ",", "enhance", "EKLF", "'s", "transcriptional", "activation", "of", "the", "beta-globin", "promoter", "in", "erythroid", "cells", "."]} {"tags": [0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["These", "results", "establish", "EKLF", "as", "a", "tissue-specific", "transcription", "factor", "that", "undergoes", "post-translational", "acetylation", "and", "suggest", "a", "mechanism", "by", "which", "EKLF", "is", "able", "to", "alter", "chromatin", "structure", "and", "induce", "beta-globin", "expression", "within", "the", "beta-like", "globin", "cluster", "."]} {"tags": [0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Recognition", "of", "herpes", "simplex", "virus", "type", "2", "tegument", "proteins", "by", "CD4", "T", "cells", "infiltrating", "human", "genital", "herpes", "lesions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "local", "cellular", "immune", "response", "to", "herpes", "simplex", "virus", "(", "HSV", ")", "is", "important", "in", "the", "control", "of", "recurrent", "HSV", "infection", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "antiviral", "functions", "of", "infiltrating", "CD4-bearing", "T", "cells", "may", "include", "cytotoxicity", ",", "inhibition", "of", "viral", "growth", ",", "lymphokine", "secretion", ",", "and", "support", "of", "humoral", "and", "CD8", "responses", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "antigens", "recognized", "by", "many", "HSV-specific", "CD4", "T", "cells", "localizing", "to", "genital", "HSV-2", "lesions", "are", "unknown", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T", "cells", "recognizing", "antigens", "encoded", "within", "map", "units", "0.67", "to", "0.73", "of", "HSV", "DNA", "are", "frequently", "recovered", "from", "herpetic", "lesions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 0, 0, 3, 4, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Expression", "cloning", "with", "this", "region", "of", "DNA", "now", "shows", "that", "tegument", "protein", "VP22", "and", "the", "viral", "dUTPase", ",", "encoded", "by", "genes", "UL49", "and", "UL50", ",", "respectively", ",", "are", "T-cell", "antigens", "."]} {"tags": [0, 3, 0, 3, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0], "tokens": ["Separate", "epitopes", "in", "VP22", "were", "defined", "for", "T-cell", "clones", "from", "each", "of", "three", "patients", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Reactivity", "with", "the", "tegument", "protein", "encoded", "by", "UL21", "was", "identified", "for", "an", "additional", "patient", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 0, 0, 3, 0], "tokens": ["Three", "new", "epitopes", "were", "identified", "in", "VP16", ",", "a", "tegument", "protein", "associated", "with", "VP22", "."]} {"tags": [0, 7, 8, 8, 8, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Some", "tegument-specific", "CD4", "T-cell", "clones", "exhibited", "cytotoxic", "activity", "against", "HSV-infected", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "suggest", "that", "herpes", "simplex", "tegument", "proteins", "are", "processed", "for", "antigen", "presentation", "in", "vivo", "and", "are", "possible", "candidate", "compounds", "for", "herpes", "simplex", "vaccines", "."]} {"tags": [3, 0, 3, 4, 4, 4, 0, 5, 6, 0], "tokens": ["Fibrinogen", "activates", "NF-kappa", "B", "transcription", "factors", "in", "mononuclear", "phagocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Adhesion", "to", "extracellular", "matrices", "is", "known", "to", "modulate", "leukocyte", "activation", ",", "although", "the", "mechanisms", "are", "not", "fully", "understood", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Mononuclear", "phagocytes", "are", "exposed", "to", "fibrinous", "provisional", "matrix", "throughout", "migration", "into", "inflammatory", "foci", ",", "so", "this", "study", "was", "undertaken", "to", "determine", "whether", "fibrinogen", "triggers", "activation", "of", "NF-kappa", "B", "transcription", "factors", "."]} {"tags": [7, 8, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["U937", "cells", "differentiated", "with", "PMA", "in", "nonadherent", "culture", "were", "shown", "to", "express", "two", "fibrinogen-binding", "integrins", ",", "predominately", "CD11b/CD18", ",", "and", "to", "a", "lesser", "extent", ",", "CD11c/CD18", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cells", "stimulated", "with", "fibrinogen", "(", "10-100", "microg/ml", ")", "/Mn2+", "(", "50", "microM", ")", "for", "2", "h", "were", "examined", "by", "electrophoretic", "mobility", "shift", "assay", "."]} {"tags": [3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0], "tokens": ["NF-kappa", "B", "activation", ",", "minimal", "in", "unstimulated", "cells", ",", "was", "substantially", "up-regulated", "by", "fibrinogen", "."]} {"tags": [3, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0], "tokens": ["Fibrinogen", "also", "caused", "activation", "of", "AP-1", ",", "but", "not", "SP1", "or", "cAMP", "response", "element-binding", "protein", "(", "CREB", ")", "factors", "."]} {"tags": [0, 3, 0, 3, 0, 3, 0, 3, 0, 3, 4, 0, 0], "tokens": ["Blocking", "mAbs", "against", "CD18", "and", "CD11b", "abrogated", "fibrinogen", "-induced", "NF-kappa", "B", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0], "tokens": ["To", "determine", "the", "effects", "on", "transcriptional", "regulation", ",", "U937", "cells", "were", "transfected", "with", "a", "plasmid", "containing", "the", "HIV-1", "enhancer", "(", "bearing", "two", "NF-kappa", "B", "sites", ")", "coupled", "to", "a", "chloramphenicol", "acetyltransferase", "(", "CAT", ")", "reporter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cells", "were", "subsequently", "stimulated", "with", "1", ")", "PMA", "for", "24", "h", ",", "inducing", "CAT", "activity", "by", "2.6-fold", ",", "2", ")", "fibrinogen", "/Mn2+", "for", "2", "h", ",", "inducing", "CAT", "activity", "by", "3.2-fold", ",", "or", "3", ")", "costimulation", "with", "fibrinogen", "and", "PMA", ",", "inducing", "5.7-fold", "the", "CAT", "activity", "induced", "by", "PMA", "alone", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 0], "tokens": ["We", "conclude", "that", "contact", "with", "fibrinogen-derived", "proteins", "may", "contribute", "to", "mononuclear", "phagocyte", "activation", "by", "signaling", "through", "CD11b/CD18", ",", "resulting", "in", "selective", "activation", "of", "transcriptional", "regulatory", "factors", ",", "including", "NF-kappa", "B", "."]} {"tags": [5, 6, 6, 6, 6, 6, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Peripheral", "blood", "T", "cells", "and", "monocytes", "and", "B", "cell", "lines", "derived", "from", "patients", "with", "lupus", "express", "estrogen", "receptor", "transcripts", "similar", "to", "those", "of", "normal", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["OBJECTIVE", ":", "To", "identify", "and", "characterize", "estrogen", "receptor", "(", "ER", ")", "transcripts", "expressed", "in", "immune", "cells", "of", "patients", "with", "systemic", "lupus", "erythematosus", "(", "SLE", ")", "and", "healthy", "donors", "."]} {"tags": [0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Peripheral", "blood", "monocytes", "and", "T", "cells", "were", "prepared", "from", "patients", "with", "SLE", "(", "n", "=", "6", ")", "and", "healthy", "donors", "(", "n", "=", "8", ")", "."]} {"tags": [5, 6, 0, 0, 0, 5, 0, 5, 0], "tokens": ["T", "cells", "were", "separated", "into", "CD4", "and", "CD8", "."]} {"tags": [0, 5, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Some", "monocytes", "and", "T", "cells", "were", "stimulated", "with", "estradiol", ",", "PMA", ",", "and", "ionomycin", "."]} {"tags": [0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Epstein-Barr", "virus-transformed", "B", "cell", "lines", "(", "n", "=", "7", ")", "and", "B", "cell", "hybridomas", "(", "n", "=", "2", ")", "established", "from", "patients", "with", "SLE", "and", "a", "healthy", "individual", "were", "used", "as", "a", "B", "cell", "source", "."]} {"tags": [0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "cells", "were", "examined", "for", "ER", "mRNA", "by", "reverse", "transcription", "nested", "polymerase", "chain", "reaction", "."]} {"tags": [0, 1, 0, 0, 0, 0, 0, 0], "tokens": ["Amplified", "cDNA", "were", "sequenced", "by", "standard", "methods", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "In", "all", "cells", "tested", ",", "ER", "mRNA", "was", "expressed", "without", "prior", "in", "vitro", "stimulation", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0], "tokens": ["Partial", "sequences", "from", "exons", "1-8", "were", "nearly", "identical", "to", "the", "published", "sequence", "of", "the", "human", "ER", "mRNA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0], "tokens": ["There", "were", "no", "notable", "differences", "in", "the", "ER", "transcripts", "between", "patients", "and", "healthy", "controls", "."]} {"tags": [0, 0, 0, 0, 1, 2, 0, 1, 2, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Variant", "receptor", "transcripts", "lacking", "exon", "5", "or", "exon", "7", ",", "which", "encodes", "the", "hormone", "binding", "domain", ",", "were", "identified", "in", "the", "majority", "of", "the", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Precise", "deletion", "of", "the", "exons", "suggests", "that", "they", "are", "alternatively", "spliced", "transcripts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0], "tokens": ["Whether", "the", "detected", "transcripts", "are", "translated", "into", "functional", "receptor", "proteins", "remains", "to", "be", "determined", "."]} {"tags": [0, 0, 0, 0, 0, 0, 9, 10, 0, 0], "tokens": ["In", "vitro", "stimulation", "did", "not", "affect", "ER", "mRNA", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "presence", "of", "variants", "did", "not", "correlate", "with", "disease", "activity", "or", "medication", "."]} {"tags": [0, 0, 5, 0, 5, 6, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 4, 4, 0, 0, 0], "tokens": ["CONCLUSION", ":", "Monocytes", ",", "T", "cells", ",", "and", "B", "cells", "in", "patients", "express", "transcripts", "of", "the", "normal", "wild", "type", "ER", "and", "the", "hormone", "binding", "domain", "variants", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 3, 4, 0, 3, 0], "tokens": ["DNA", "damaging", "agents", "induce", "expression", "of", "Fas", "ligand", "and", "subsequent", "apoptosis", "in", "T", "lymphocytes", "via", "the", "activation", "of", "NF-kappa", "B", "and", "AP-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Apoptosis", "induced", "by", "DNA", "damage", "and", "other", "stresses", "can", "proceed", "via", "expression", "of", "Fas", "ligand", "(", "FasL", ")", "and", "ligation", "of", "its", "receptor", ",", "Fas", "(", "CD95", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "report", "that", "activation", "of", "the", "two", "transcription", "factors", "NF-kappa", "B", "and", "AP-1", "is", "crucially", "involved", "in", "FasL", "expression", "induced", "by", "etoposide", ",", "teniposide", ",", "and", "UV", "irradiation", "."]} {"tags": [0, 3, 4, 0, 3, 4, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "nondegradable", "mutant", "of", "I", "kappa", "B", "blocked", "both", "FasL", "expression", "and", "apoptosis", "induced", "by", "DNA", "damage", "but", "not", "Fas", "ligation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "stimuli", "also", "induced", "the", "stress-activated", "kinase", "pathway", "(", "SAPK/JNK", ")", ",", "which", "was", "required", "for", "the", "maximal", "induction", "of", "apoptosis", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0], "tokens": ["A", "1.2", "kb", "FasL", "promoter", "responded", "to", "DNA", "damage", ",", "as", "well", "as", "coexpression", "with", "p65", "Rel", "or", "Fos/Jun", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0], "tokens": ["Mutations", "in", "the", "relevant", "NF-kappa", "B", "and", "AP-1", "binding", "sites", "eliminated", "these", "responses", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Thus", ",", "activation", "of", "NF-kappa", "B", "and", "AP-1", "contributes", "to", "stress-induced", "apoptosis", "via", "the", "expression", "of", "FasL", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["A", "small", ",", "nonpeptidyl", "mimic", "of", "granulocyte-colony-stimulating", "factor", "[", "see", "commetns", "]"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["A", "nonpeptidyl", "small", "molecule", "SB", "247464", ",", "capable", "of", "activating", "granulocyte-colony-stimulating", "factor", "(", "G-CSF", ")", "signal", "transduction", "pathways", ",", "was", "identified", "in", "a", "high-throughput", "assay", "in", "cultured", "cells", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 5, 6, 6, 6, 6, 0, 0, 5, 6, 0, 0, 0], "tokens": ["Like", "G-CSF", ",", "SB", "247464", "induced", "tyrosine", "phosphorylation", "of", "multiple", "signaling", "proteins", "and", "stimulated", "primary", "murine", "bone", "marrow", "cells", "to", "form", "granulocytic", "colonies", "in", "vitro", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["It", "also", "elevated", "peripheral", "blood", "neutrophil", "counts", "in", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "extracellular", "domain", "of", "the", "murine", "G-CSF", "receptor", "was", "required", "for", "the", "activity", "of", "SB", "247464", ",", "suggesting", "that", "the", "compound", "acts", "by", "oligomerizing", "receptor", "chains", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "results", "indicate", "that", "a", "small", "molecule", "can", "activate", "a", "receptor", "that", "normally", "binds", "a", "relatively", "large", "protein", "ligand", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 9, 10, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0], "tokens": ["Minimal", "residual", "disease", "in", "acute", "myelogenous", "leukemia", "with", "PML/RAR", "alpha", "or", "AML1/ETO", "mRNA", "and", "phenotypic", "analysis", "of", "possible", "T", "and", "natural", "killer", "cells", "in", "bone", "marrow", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "studied", "minimal", "residual", "disease", "(", "MRD", ")", "of", "patients", "with", "acute", "myeloid", "leukemia", "(", "AML", ")", "who", "have", "PML/RAR", "alpha", "or", "AML1/ETO", "as", "well", "as", "the", "phenotypic", "analysis", "of", "lymphocyte", "subsets", "involved", "in", "antitumor", "immunity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Eight", "patients", "in", "long-term", "(", "LT", ";", "3", "to", "15", "years", ")", "and", "15", "patients", "in", "short-term", "(", "ST", ";", "up", "to", "3", "years", ")", "remission", "were", "studied", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0], "tokens": ["Using", "the", "reverse", "transcription-polymerase", "chain", "reaction", "(", "RT", ")", "assay", ",", "the", "limit", "of", "detection", "was", "10", "(", "-5", ")", "to", "10", "(", "-6", ")", "for", "PML/RAR", "alpha", "transcript", "and", "10", "(", "-4", ")", "to", "10", "(", "-5", ")", "for", "the", "AML1/ETO", "transcript", "."]} {"tags": [0, 0, 5, 6, 6, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Simultaneously", ",", "T", "lymphocyte", "subsets", "and", "NK", "cells", "from", "the", "peripheral", "blood", "(", "PB", ")", "and", "bone", "marrow", "(", "BM", ")", "were", "investigated", "by", "flow", "cytometric", "analysis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Four", "of", "the", "eight", "patients", "in", "LT", "and", "7", "of", "the", "15", "patients", "in", "ST", "remission", "were", "MRD-positive", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "all", "MRD-positive", "patients", "in", "LT", "remission", "are", "still", "until", "now", "event-free", ",", "3", "of", "the", "7", "MRD-positive", "(", "MRD+", ")", "patients", "in", "ST", "remission", "soon", "relapsed", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 3, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "total", "populations", "of", "CD4", "+", ",", "CD8", "+", "and", "CD56", "+", "[", "possible", "T-cell", "and", "natural", "killer", "(", "T/NK", ")", "populations", "]", "in", "the", "BM", "of", "ST", "patients", "and", "MRD+/LT", "patients", "were", "significantly", "(", "p", "<", ".01", ")", "low", "."]} {"tags": [0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "CD8+", "CD28+", "population", "showed", "the", "same", "tendency", "(", "p", "<", ".01-.02", ")", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "T/NK", "subsets", "in", "the", "BM", "of", "MRD-negative", "(", "MRD-", ")", "LT", "(", "MRD-/LT", ")", "patients", "showed", "similar", "numbers", "of", "cells", "as", "normal", "volunteers", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Basically", ",", "the", "total", "percentage", "of", "the", "CD4+", ",", "CD8+", "and", "CD56+", "cell", "populations", "in", "the", "BM", "was", "increased", "and", "in", "the", "following", "order", ":", "MRD-/LT", "patients", ",", "normal", "volunteers", ",", "MRD+/LT", "patients", "and", "MRD+", "or", "-/ST", "patients", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "percentages", "of", "the", "T/NK-cell", "subsets", "in", "the", "PB", "were", "not", "significantly", "different", "among", "these", "groups", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "the", "difference", "of", "the", "possible", "T/NK-cell", "phenotype", "in", "the", "BM", "may", "strongly", "influence", "clinical", "and", "molecular", "remission", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0, 0], "tokens": ["These", "results", "still", "remain", "to", "be", "confirmed", "by", "further", "studies", "of", "the", "functional", "anti-tumor", "immunity", "of", "T/NK", "cells", "of", "AML", "in", "remission", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 5, 6, 0], "tokens": ["Mycobacterium", "tuberculosis", "mannose-capped", "lipoarabinomannan", "can", "induce", "NF-kappaB", "-dependent", "activation", "of", "human", "immunodeficiency", "virus", "type", "1", "long", "terminal", "repeat", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tuberculosis", "has", "emerged", "as", "an", "epidemic", ",", "extended", "by", "the", "large", "number", "of", "individuals", "infected", "with", "human", "immunodeficiency", "virus", "type", "1", "(", "HIV-1", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "major", "goal", "of", "this", "study", "was", "to", "determine", "whether", "the", "mycobacterial", "cell", "wall", "component", "mannose-capped", "lipoarabinomannan", "(", "ManLAM", ")", "of", "Mycobacterium", "tuberculosis", "(", "M.", "tuberculosis", ")", "could", "activate", "transcription", "of", "HIV-1", "in", "T", "cells", "with", "the", "use", "of", "an", "in", "vitro", "cell", "culture", "system", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "experiments", "are", "of", "prime", "importance", "considering", "that", "CD4", "-expressing", "T", "lymphocytes", "represent", "the", "major", "virus", "reservoir", "in", "the", "peripheral", "blood", "of", "infected", "individuals", "."]} {"tags": [0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "the", "1G5", "cell", "line", "harbouring", "the", "luciferase", "reporter", "gene", "under", "the", "control", "of", "the", "HIV-1", "LTR", ",", "it", "was", "first", "found", "that", "culture", "protein", "filtrates", "(", "CFP", ")", "from", "M.", "tuberculosis", "or", "purified", "ManLAM", "could", "activate", "HIV-1", "LTR-dependent", "gene", "expression", "unlike", "similarly", "prepared", "CFP", "extracts", "devoid", "of", "ManLAM", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 4, 4, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "implication", "of", "protein", "tyrosine", "kinase", "(", "s", ")", ",", "protein", "kinase", "A", "and/or", "protein", "kinase", "C", "was", "highlighted", "by", "the", "abrogation", "of", "the", "ManLAM-mediated", "activation", "of", "HIV-1", "LTR-driven", "gene", "expression", "using", "herbimycin", "A", "and", "H7", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["It", "was", "also", "determined", ",", "using", "electrophoresis", "mobility", "shift", "assays", ",", "that", "M.", "tuberculosis", "ManLAM", "led", "to", "the", "nuclear", "translocation", "of", "the", "transcription", "factor", "NF-kappaB", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0], "tokens": ["M.", "tuberculosis", "ManLAM", "resulted", "in", "clear", "induction", "of", "the", "luciferase", "gene", "placed", "under", "the", "control", "of", "the", "wild-type", ",", "but", "not", "the", "kappaB-mutated", ",", "HIV-1", "LTR", "region", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Finally", ",", "the", "ManLAM-mediated", "activation", "of", "HIV-1", "LTR", "transcription", "was", "found", "to", "be", "independent", "of", "the", "autocrine", "or", "paracrine", "action", "of", "endogenous", "TNF-alpha", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "results", "suggest", "that", "M.", "tuberculosis", "can", "upregulate", "HIV-1", "expression", "in", "T", "cells", "and", "could", "thus", "have", "the", "potential", "to", "influence", "the", "pathogenesis", "of", "HIV-1", "infection", "."]} {"tags": [1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["Human", "immunodeficiency", "virus", "type", "1", "long", "terminal", "repeat", "quasispecies", "differ", "in", "basal", "transcription", "and", "nuclear", "factor", "recruitment", "in", "human", "glial", "cells", "and", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "generation", "of", "genomic", "diversity", "during", "the", "course", "of", "infection", "has", "the", "potential", "to", "affect", "all", "aspects", "of", "HIV-1", "replication", ",", "including", "expression", "of", "the", "proviral", "genome", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "gain", "a", "better", "understanding", "of", "the", "impact", "of", "long", "terminal", "repeat", "(", "LTR", ")", "sequence", "diversity", "on", "LTR-directed", "gene", "expression", "in", "cells", "of", "the", "central", "nervous", "system", "(", "CNS", ")", "and", "immune", "system", ",", "we", "amplified", "and", "cloned", "LTRs", "from", "proviral", "DNA", "in", "HIV-1-infected", "peripheral", "blood", "."]} {"tags": [0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Sequence", "analysis", "of", "nineteen", "LTRs", "cloned", "from", "2", "adult", "and", "3", "pediatric", "patients", "revealed", "an", "average", "of", "33", "nucleotide", "changes", "(", "with", "respect", "to", "the", "sequence", "of", "the", "LAI", "LTR", ")", "within", "the", "455-bp", "U3", "region", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 7, 8, 8, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 7, 8, 0, 0, 7, 8, 8, 8, 0, 0], "tokens": ["Transient", "expression", "analyses", "in", "cells", "of", "neuroglial", "and", "lymphocytic", "origin", "demonstrated", "that", "some", "of", "these", "LTRs", "had", "activities", "which", "varied", "significantly", "from", "the", "LAI", "LTR", "in", "U-373", "MG", "cells", "(", "an", "astrocytoma", "cell", "line", ")", "as", "well", "as", "in", "Jurkat", "cells", "(", "a", "CD4-positive", "lymphocyte", "cell", "line", ")", "."]} {"tags": [0, 1, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 7, 8, 0, 0, 1, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 1, 2, 0], "tokens": ["While", "LTRs", "which", "demonstrated", "the", "highest", "activities", "in", "U-373", "MG", "cells", "also", "yielded", "high", "activities", "in", "Jurkat", "cells", ",", "the", "LTRs", "were", "generally", "more", "active", "in", "Jurkat", "cells", "when", "compared", "to", "the", "LAI", "LTR", "."]} {"tags": [0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Differences", "in", "LTR", "sequence", "also", "resulted", "in", "differences", "in", "transcription", "factor", "recruitment", "to", "cis-acting", "sites", "within", "the", "U3", "region", "of", "the", "LTR", ",", "as", "demonstrated", "by", "electrophoretic", "mobility", "shift", "assays", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["In", "particular", ",", "naturally", "occurring", "sequence", "variation", "impacted", "transcription", "factor", "binding", "to", "an", "activating", "transcription", "factor", "/", "cAMP", "response", "element", "binding", "(", "ATF", "/", "CREB", ")", "binding", "site", "(", "located", "between", "the", "LEF-1", "and", "distal", "NF-kappaB", "transcription", "factor", "binding", "sites", ")", "that", "we", "identified", "in", "previous", "studies", "of", "the", "HIV-1", "LTR", "."]} {"tags": [0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "findings", "suggest", "that", "LTR", "sequence", "changes", "can", "significantly", "affect", "basal", "LTR", "function", "and", "transcription", "factor", "recruitment", ",", "which", "may", ",", "in", "turn", ",", "alter", "the", "course", "of", "viral", "replication", "in", "cells", "of", "CNS", "and", "immune", "system", "origin", "."]} {"tags": [3, 4, 4, 4, 4, 0, 5, 0, 0], "tokens": ["HMG", "box", "containing", "transcription", "factors", "in", "lymphocyte", "differentiation", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "identification", "of", "the", "mammalian", "sex-determining", "gene", "Sry", "has", "led", "to", "the", "discovery", "of", "a", "large", "family", "of", "related", "(", "'", "HMG", "box", "'", ")", "transcription", "factors", "that", "control", "developmental", "events", "in", "yeast", ",", "C.", "elegans", ",", "Drosophila", "and", "vertebrates", "."]} {"tags": [0, 5, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0], "tokens": ["In", "lymphocyte", "differentiation", ",", "several", "HMG", "box", "proteins", "play", "a", "decisive", "role", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Sox-4", "is", "important", "for", "very", "early", "B-cell", "differentiation", ",", "while", "TCF-1", "/", "LEF-1", "play", "a", "crucial", "role", "in", "early", "thymocyte", "development", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["TCF/LEF", "proteins", "have", "recently", "been", "found", "to", "constitute", "a", "downstream", "component", "of", "the", "Wingless/Wnt", "signal", "transduction", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "flies", ",", "this", "pathway", "controls", "segment", "polarity", ";", "in", "Xenopus", "it", "controls", "the", "definition", "of", "the", "body", "axis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Deregulation", "of", "the", "pathway", "occurs", "in", "several", "human", "tumors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0], "tokens": ["These", "insights", "in", "the", "molecular", "events", "that", "are", "involved", "in", "TCF/LEF", "function", "in", "these", "organisms", "may", "eventually", "lead", "to", "the", "understanding", "of", "the", "function", "of", "these", "HMG", "box", "proteins", "in", "lymphoid", "development"]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["Transcriptional", "regulation", "by", "C/EBP", "alpha", "and", "-beta", "in", "the", "expression", "of", "the", "gene", "for", "the", "MRP14", "myeloid", "calcium", "binding", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0], "tokens": ["Transcriptional", "regulation", "of", "the", "gene", "for", "the", "myeloid", "calcium", "binding", "protein", ",", "MRP14", ",", "was", "investigated", "in", "human", "monocytic", "leukemia", "cell", "lines", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 7, 8, 8, 0, 7, 8, 8, 0, 0, 7, 8, 8, 0], "tokens": ["The", "MRP14", "gene", "was", "not", "expressed", "in", "monoblastic", "ML-1", "cells", ",", "promonocytic", "U-937", "cells", ",", "or", "promyelocytic", "HL-60", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["On", "the", "other", "hand", ",", "the", "gene", "was", "expressed", "in", "monocytic", "THP-1", "cells", "and", "in", "the", "HL-60", "cells", "treated", "with", "1", ",", "25-dihydroxyvitamin", "D3", "(", "VD3", ")", "."]} {"tags": [0, 0, 0, 3, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["The", "level", "of", "MRP14", "in", "VD3-treated", "HL-60", "cells", "was", "two-fold", "higher", "than", "that", "in", "THP-1", "cells", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 3, 4, 4, 4, 4, 0, 7, 8, 8, 8, 8, 8, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Among", "several", "known", "transcription", "factor", "binding", "motifs", ",", "nuclear", "protein", "(", "s", ")", "of", "VD3-treated", "HL-60", "cells", "and", "THP-1", "cells", "bound", "to", "the", "CCAAT/enhancer", "binding", "protein", "(", "C/EBP", ")", "-binding", "motif", "that", "was", "located", "in", "the", "upstream", "region", "of", "the", "MRP14", "gene", "(", "-81", ")", ",", "as", "evidenced", "by", "the", "competitive", "gel", "mobility-shift", "assay", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 7, 8, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 7, 8, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["An", "antibody", "for", "C/EBP", "alpha", "super-shifted", "the", "nucleoprotein", "complex", "in", "THP-1", "cells", "but", "not", "in", "the", "VD3-treated", "HL-60", "cells", ",", "whereas", "an", "antibody", "for", "C/EBP", "beta", "blocked", "the", "formation", "of", "the", "complex", "with", "the", "nuclear", "factor", "of", "the", "HL-60", "cells", "but", "not", "with", "that", "of", "THP-1", "cells", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["An", "anti-C/EBP", "delta", "antibody", "had", "no", "effect", "on", "the", "complex", "in", "either", "cell", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 7, 8, 0, 3, 4, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Thus", ",", "it", "was", "concluded", "that", "C/EBP", "alpha", "and", "-beta", "were", "able", "to", "bind", "to", "the", "C/EBP", "motif", ",", "and", "that", "C/EBP", "alpha", "bound", "to", "the", "motif", "in", "THP-1", "cells", "and", "C/EBP", "beta", "bound", "to", "that", "in", "the", "VD3-treated", "HL-60", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 1, 2, 2, 0, 7, 8, 0, 7, 8, 0], "tokens": ["Furthermore", ",", "to", "examine", "the", "transcriptional", "activity", "of", "the", "C/EBP", "motif", ",", "we", "transfected", "several", "constructed", "luciferase", "reporter", "DNAs", "into", "HL-60", "cells", "and", "THP-1", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 7, 8, 0, 0, 0, 0, 0, 0], "tokens": ["The", "luciferase", "activity", "of", "the", "C/EBP", "motif", "in", "HL-60", "cells", "was", "increased", "by", "VD3", "treatment", "."]} {"tags": [0, 3, 4, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0, 0, 0, 0], "tokens": ["The", "C/EBP", "motif", "in", "the", "MRP14", "gene", "was", "confirmed", "to", "function", "as", "a", "regulatory", "region", "in", "VD3-treated", "HL-60", "cells", "and", "THP-1", "cells", "by", "the", "assay", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Since", "C/EBP", "beta", "was", "also", "detected", "in", "VD3-untreated", "HL-60", "cells", "by", "immunoblotting", ",", "VD3", "activated", "C/EBP", "beta", "to", "bind", "to", "the", "motif", ",", "probably", "through", "post-translational", "modification", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Patients", "with", "high-risk", "myelodysplastic", "syndrome", "can", "have", "polyclonal", "or", "clonal", "haemopoiesis", "in", "complete", "haematological", "remission", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0], "tokens": ["The", "clonality", "of", "mature", "peripheral", "blood-derived", "myeloid", "and", "lymphoid", "cells", "and", "bone", "marrow", "haemopoietic", "progenitors", "from", "18", "females", "with", "myelodysplasia", "(", "MDS", ")", "(", "five", "refractory", "anaemia", ",", "RA", ";", "one", "RA", "with", "ringed", "sideroblasts", ",", "RARS", ";", "three", "chronic", "myelomonocytic", "leukaemia", ",", "CMML", ";", "four", "RA", "with", "excess", "of", "blasts", ",", "RAEB", ";", "five", "RAEB", "in", "transformation", ",", "RAEB-t", ")", "was", "studied", "by", "X-chromosome", "inactivation", "analysis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 5, 6, 6, 0, 5, 6, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0], "tokens": ["Using", "the", "human", "androgen-receptor", "(", "HUMARA", ")", "assay", ",", "we", "analysed", "the", "clonal", "patterns", "of", "highly", "purified", "immature", "CD34+", "38-", "and", "committed", "CD34+", "38+", "marrow-derived", "progenitors", ",", "and", "CD16+", "14-", "granulocytes", ",", "CD14+", "monocytes", ",", "CD3+", "T", "and", "CD19+", "B", "lymphocytes", "from", "peripheral", "blood", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "high-risk", "patients", "(", "RAEB", ",", "RAEB-t", ")", ",", "clonality", "analysis", "was", "performed", "before", "and", "after", "intensive", "remission-induction", "treatment", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 5, 0], "tokens": ["All", "patients", ",", "except", "one", "with", "RA", ",", "had", "predominance", "of", "a", "single", "clone", "in", "their", "granulocytes", "and", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["The", "same", "clonal", "pattern", "was", "found", "in", "CD34+", "progenitor", "cells", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "CD3+", "T", "lymphocytes", "were", "polyclonal", "or", "oligoclonal", "in", "14/18", "patients", "."]} {"tags": [1, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["X-chromosome", "inactivation", "patterns", "of", "CD19+", "B", "cells", "were", "highly", "concordant", "with", "CD3+", "T", "cells", "except", "for", "two", "patients", "(", "one", "RA", ",", "one", "CMML", ")", "with", "monoclonal", "B", "and", "polyclonal", "T", "lymphocytes", ",", "therefore", "suggesting", "a", "clonal", "mutation", "in", "a", "progenitor", "common", "to", "the", "myeloid", "and", "B-lymphoid", "lineages", "or", "the", "coexistence", "of", "MDS", "and", "a", "B-cell", "disorder", "in", "these", "particular", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["After", "high-dose", "non-myeloablative", "chemotherapy", ",", "polyclonal", "haemopoiesis", "was", "reinstalled", "in", "the", "mature", "myeloid", "cells", "and", "immature", "and", "committed", "marrow", "progenitors", "in", "three", "of", "four", "patients", "achieving", "complete", "haematological", "remission", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", "we", "conclude", "that", "most", "haematological", "remissions", "in", "MDS", "are", "associated", "with", "restoration", "of", "polyclonal", "haemopoiesis", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Prominent", "sex", "steroid", "metabolism", "in", "human", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 7, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0], "tokens": ["Steroid", "metabolism", "was", "investigated", "in", "cultured", "human", "B-lymphoblastoid", "cells", "(", "B-LCL", ")", ",", "and", "peripheral", "blood", "T", "and", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Gene", "expression", "was", "examined", "by", "reverse-transcription", "polymerase", "chain", "reaction", "amplification", "(", "RT-PCR", ")", "."]} {"tags": [0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 3, 0, 3, 0, 3, 0, 3, 4, 4, 0, 0, 3, 0, 3, 4, 4, 4, 0, 0, 3, 0, 3, 4, 0, 0], "tokens": ["Appropriate", "sized", "transcripts", "were", "detected", "in", "both", "cultured", "and", "fresh", "peripheral", "lymphocytes", "for", "CYP11A", ",", "CYP17", ",", "HSD11L", "(", "11beta-hydroxysteroid", "dehydrogenase", "I", ")", ",", "HSD17B1", "(", "17beta-hydroxysteroid", "dehydrogenase", "type", "I", ")", "and", "SRD5A1", "(", "5alpha-reductase", "I", ")", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 3, 0], "tokens": ["B-LCL", ",", "but", "not", "T", "and", "B", "cells", ",", "expressed", "CYP11B", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 4, 4, 4, 4, 0, 0, 7, 0, 5, 6, 0], "tokens": ["There", "was", "minimal", "expression", "of", "HSD3B1", "and", "HSD3B2", "(", "3beta-hydroxysteroid", "dehydrogenase", "I", "and", "II", ")", "in", "B-LCL", "and", "T", "cells", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 0, 0], "tokens": ["Transcripts", "for", "CYP19", "and", "HSD11K", "were", "not", "detected", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Corresponding", "enzymatic", "activity", "was", "detectable", "only", "for", "17-hydroxysteroid", "dehydrogenase", "and", "5alpha-reductase", ",", "respectively", "producing", "testosterone", "and", "5alpha-dihydrotestosterone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Steroid", "identities", "were", "confirmed", "by", "gas", "chromatography/mass", "spectrometry", "(", "GC/MS", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["One", "metabolite", "thought", "to", "be", "deoxycorticosterone", "was", "identified", "by", "GC/MS", "as", "6alpha-hydroxypregnanolone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "was", "concluded", "that", "sex", "hormone", "metabolism", ",", "including", "androgen", "synthesis", ",", "occurs", "in", "lymphocytes", ",", "and", "may", "modulate", "immune", "response", "."]} {"tags": [5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0], "tokens": ["Human", "white", "blood", "cells", "and", "hair", "follicles", "are", "good", "sources", "of", "mRNA", "for", "the", "pterin", "carbinolamine", "dehydratase/dimerization", "cofactor", "of", "HNF1", "for", "mutation", "detection", "."]} {"tags": [3, 4, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Pterin", "carbinolamine", "dehydratase/dimerization", "cofactor", "of", "HNF1", "(", "PCD/DCoH", ")", "is", "a", "protein", "that", "has", "a", "dual", "function", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "is", "a", "pterin", "4alpha-carbinolamine", "dehydratase", "that", "is", "involved", "in", "the", "regeneration", "of", "the", "cofactor", "tetrahydrobiopterin", "during", "the", "phenylalanine", "hydroxylase-", "catalyzed", "hydroxylation", "of", "phenylalanine", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["In", "addition", ",", "it", "is", "the", "dimerization", "cofactor", "of", "HNF1", "that", "is", "able", "to", "activate", "the", "transcriptional", "activity", "of", "HNF1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["Deficiencies", "in", "the", "gene", "for", "this", "dual", "functional", "protein", "result", "in", "hyperphenylalaninemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0], "tokens": ["Here", "we", "report", "for", "the", "first", "time", "that", "the", "PCD/DCoH", "mRNA", "is", "present", "in", "human", "white", "blood", "cells", "and", "hair", "follicles", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Taking", "advantage", "of", "this", "finding", ",", "a", "sensitive", ",", "rapid", "and", "convenient", "method", "for", "screening", "mutations", "occurring", "in", "the", "coding", "region", "of", "this", "gene", "has", "been", "described", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Copyright", "1998", "Academic", "Press", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0], "tokens": ["Biochemical", "characterization", "of", "the", "NF-Y", "transcription", "factor", "complex", "during", "B", "lymphocyte", "development", "."]} {"tags": [0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0], "tokens": ["The", "transcription", "factor", ",", "NF-Y", ",", "plays", "a", "critical", "role", "in", "tissue-specific", "major", "histocompatibility", "complex", "class", "II", "gene", "transcription", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "report", "the", "biochemical", "properties", "of", "the", "heterotrimeric", "NF-Y", "complex", "have", "been", "characterized", "during", "stage-specific", "B-cell", "development", ",", "and", "in", "several", "class", "II-", "mutant", "B-cell", "lines", ",", "which", "represent", "distinct", "bare", "lymphocyte", "syndrome", "class", "II", "genetic", "complementation", "groups", "."]} {"tags": [0, 3, 4, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 0, 0, 5, 6, 6, 6, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 0, 7, 0, 7, 0, 0, 0, 0, 3, 0, 3, 4, 4, 3, 4, 0], "tokens": ["The", "NF-Y", "complex", "derived", "from", "class", "II+", "mature", "B-cells", "bound", "with", "high", "affinity", "to", "anion", "exchangers", ",", "and", "eluted", "as", "an", "intact", "trimeric", "complex", ",", "whereas", ",", "NF-Y", "derived", "from", "class", "II-", "plasma", "B-cells", ",", "and", "from", "bare", "lymphocyte", "syndrome", "group", "II", "cell", "lines", ",", "RJ2.2.5", "and", "RM3", ",", "dissociated", "into", "discrete", "NF-YA", "and", "NF-YB", ":", "C", "subunit", "fractions", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0], "tokens": ["Recombination", "of", "the", "MPC11", "plasma", "B-cell", "derived", "NF-Y", "A", ":", "B", ":", "C", "complex", "with", "the", "low", "molecular", "mass", "protein", "fraction", ",", "NF-Y-associated", "factors", "(", "YAFs", ")", ",", "derived", "from", "mature", "A20", "B-cell", "nuclei", ",", "conferred", "high", "affinity", "anion", "exchange", "binding", "to", "NF-Y", "as", "an", "intact", "trimeric", "complex", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 3, 4, 0, 3, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 0, 0, 0, 3, 0, 0, 1, 2, 2, 0, 0, 0], "tokens": ["Recombination", "of", "the", "native", "NF-YA", ":", "B", ":", "C", "complex", "with", "the", "transcriptional", "cofactor", ",", "PC4", ",", "likewise", "conferred", "high", "affinity", "NF-Y", "binding", "to", "anion", "exchangers", ",", "and", "stabilized", "NF-Y", "interaction", "with", "CCAAT-box", "DNA", "motifs", "in", "vitro", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 3, 0], "tokens": ["Interaction", "between", "PC4", "and", "NF-Y", "was", "mapped", "to", "the", "C-terminal", "region", "of", "PC4", ",", "and", "the", "subunit", "interaction", "subdomain", "of", "the", "highly", "conserved", "DNA", "binding-subunit", "interaction", "domain", "(", "DBD", ")", "of", "NF-YA", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 6, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["These", "results", "suggest", "that", "in", "class", "II+", "mature", "B-cells", "NF-Y", "is", "associated", "with", "the", "protein", "cofactor", ",", "PC4", ",", "which", "may", "play", "an", "important", "role", "in", "NF-Y-mediated", "transcriptional", "control", "of", "class", "II", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Arrest", "of", "B", "lymphocyte", "terminal", "differentiation", "by", "CD40", "signaling", ":", "mechanism", "for", "lack", "of", "antibody-secreting", "cells", "in", "germinal", "centers", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Despite", "extensive", "research", ",", "the", "role", "of", "CD40", "signaling", "in", "B", "cell", "terminal", "differentiation", "remains", "controversial", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "show", "that", "CD40", "engagement", "arrests", "B", "cell", "differentiation", "prior", "to", "plasma", "cell", "formation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 0], "tokens": ["This", "arrest", "is", "manifested", "at", "a", "molecular", "level", "as", "a", "reduction", "in", "mRNA", "levels", "of", "secretory", "immunoglobulin", "gene", "products", "such", "as", "mu", "(", "s", ")", "and", "J", "chain", "as", "well", "as", "the", "loss", "of", "the", "transcriptional", "regulator", "BLIMP-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0], "tokens": ["Furthermore", ",", "the", "inhibition", "of", "B", "cell", "differentiation", "by", "CD40", "engagement", "could", "not", "be", "overcome", "by", "either", "mitogens", "or", "cytokines", ",", "but", "could", "be", "reversed", "by", "antibodies", "that", "interfere", "with", "the", "CD40", "/", "gp39", "interaction", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["These", "data", "suggest", "that", "secretory", "immunoglobulin", "is", "not", "produced", "by", "B", "cells", "that", "are", "actively", "engaged", "by", "gp39-expressing", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "positively", "charged", "alpha-lipoic", "acid", "analogue", "with", "increased", "cellular", "uptake", "and", "more", "potent", "immunomodulatory", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["alpha-Lipoic", "acid", "(", "LA", ")", "is", "taken", "up", "by", "cells", "and", "reduced", "to", "its", "potent", "dithiol", "form", ",", "dihydrolipoate", "(", "DHLA", ")", ",", "much", "of", "which", "is", "rapidly", "effluxed", "out", "from", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "improve", "retention", "in", "cells", ",", "the", "LA", "molecule", "was", "modified", "to", "confer", "a", "positive", "charge", "at", "physiological", "pH", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["N", ",", "N-dimethyl", ",", "N'-2-amidoethyl-lipoate", "was", "synthesized", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "protonated", "form", "of", "the", "new", "molecule", "is", "referred", "to", "as", "LA-Plus", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "uptake", "of", "LA-Plus", "by", "human", "Wurzburg", "T", "cells", "was", "higher", "compared", "to", "that", "of", "LA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Several-fold", "higher", "amounts", "of", "DHLA-Plus", ",", "the", "corresponding", "reduced", "form", "of", "LA-Plus", ",", "were", "detected", "in", "LA-Plus", "treated", "cells", "compared", "to", "the", "amount", "of", "DHLA", "found", "in", "cells", "treated", "with", "LA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 3, 4, 0, 0], "tokens": ["At", "100", "microM", ",", "LA", "did", "not", "but", "LA-Plus", "inhibited", "H2O2", "induced", "NF-kappaB", "activation", "and", "NF-kappaB", "directed", "IL-2", "receptor", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Both", "LA", "and", "LA-Plus", "synergised", "with", "selenium", "in", "inhibiting", "H2O2", "induced", "NF-kappaB", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0], "tokens": ["At", "150", "microM", "LA-Plus", ",", "but", "not", "LA", ",", "inhibited", "TNFalpha", "induced", "NF-kappaB", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["At", "5", "microM", "LA-Plus", ",", "but", "not", "LA", ",", "protected", "against", "both", "spontaneous", "and", "etoposide", "induced", "apoptosis", "in", "rat", "thymocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["LA-Plus", "is", "thus", "an", "improved", "form", "of", "LA", "with", "increased", "therapeutic", "potential", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Copyright", "1998", "Academic", "Press", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Carrier", "identification", "in", "X-linked", "immunodeficiency", "diseases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["OBJECTIVE", ":", "Carrier", "identification", "in", "X-linked", "immunodeficiency", "disorders", "can", "be", "based", "on", "the", "demonstration", "of", "non-random", "X", "inactivation", "(", "NRXI", ")", "in", "affected", "blood", "cell", "lineages", "when", "growth", "is", "impaired", "in", "cells", "expressing", "the", "abnormal", "gene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "examined", "the", "utility", "of", "seeking", "evidence", "of", "NRXI", "to", "test", "the", "carrier", "status", "of", "women", "in", "families", "affected", "by", "X-linked", "severe", "combined", "immunodeficiency", "(", "XSCID", ")", "and", "X-linked", "hypogammaglobulinaemia", "(", "XLH", ")", ",", "to", "identify", "as", "carriers", "the", "mothers", "of", "boys", "with", "SCID", "or", "hypogammaglobulinaemia", "whose", "phenotype", "suggested", "X-linkage", "and", "to", "infer", "X-linkage", "in", "boys", "with", "SCID", "or", "hypogammaglobulinaemia", "whose", "disease", "was", "not", "clearly", "X-linked", "on", "the", "basis", "either", "of", "family", "history", "or", "clinical", "and", "immunological", "characteristics", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["METHODOLOGY", ":", "A", "polymerase", "chain", "reaction-based", "method", "was", "used", "to", "amplify", "a", "polymorphic", "CAG", "repeat", "in", "the", "first", "exon", "of", "the", "androgen", "receptor", "gene", "after", "selective", "digestion", "of", "the", "active", "X", "chromosome", "with", "a", "methylation-sensitive", "enzyme", ",", "HpaII", "to", "distinguish", "between", "the", "paternal", "and", "maternal", "alleles", "and", "to", "identify", "their", "methylation", "status", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "Heterozygosity", "was", "found", "in", "24", "of", "31", "female", "subjects", "(", "77", "%", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["As", "anticipated", ",", "NRXI", "could", "be", "demonstrated", "in", "all", "lymphoid", "cells", "studied", "from", "obligate", "carriers", "of", "XSCID", "and", "an", "obligate", "carrier", "of", "XLH", "but", "not", "on", "a", "carrier", "of", "X-linked", "immunodeficiency", "with", "hyper-IgM", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "finding", "of", "NRXI", "in", "the", "mother", "of", "a", "boy", "with", "a", "SCID", "variant", "showed", "her", "to", "be", "a", "carrier", "of", "XSCID", "and", "establishes", "that", "her", "son", "has", "XSCID", ",", "not", "otherwise", "evident", "from", "available", "data", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSIONS", ":", "This", "PCR", "assay", "provides", "a", "rapid", "method", "for", "carrier", "detection", "of", "X-linked", "immunodeficiencies", ",", "and", "has", "allowed", "us", "to", "expand", "the", "phenotype", "of", "XSCID"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Leukocyte-endothelial", "interaction", "is", "augmented", "by", "high", "glucose", "concentrations", "and", "hyperglycemia", "in", "a", "NF-kB-dependent", "fashion", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "addressed", "the", "role", "of", "hyperglycemia", "in", "leukocyte-endothelium", "interaction", "under", "flow", "conditions", "by", "exposing", "human", "umbilical", "vein", "endothelial", "cells", "for", "24", "h", "to", "normal", "(", "5", "mM", ")", ",", "high", "concentration", "of", "glucose", "(", "30", "mM", ")", ",", "advanced", "glycosylation", "end", "product-albumin", "(", "100", "microg/ml", ")", ",", "or", "hyperglycemic", "(", "174-316", "mg/dl", ")", "sera", "from", "patients", "with", "diabetes", "and", "abnormal", "hemoglobin", "A1c", "(", "8.1+/-1.4", "%", ")", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["At", "the", "end", "of", "incubation", "endothelial", "cells", "were", "perfused", "with", "total", "leukocyte", "suspension", "in", "a", "parallel", "plate", "flow", "chamber", "under", "laminar", "flow", "(", "1.5", "dyn/cm2", ")", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Rolling", "and", "adherent", "cells", "were", "evaluated", "by", "digital", "image", "processing", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Results", "showed", "that", "30", "mM", "glucose", "significantly", "(", "P", "<", "0.01", ")", "increased", "the", "number", "of", "adherent", "leukocytes", "to", "endothelial", "cells", "in", "respect", "to", "control", "(", "5", "mM", "glucose", ";", "151+/-19", "versus", "33+/-8", "cells/mm2", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "similar", "response", "was", "induced", "by", "endothelial", "stimulation", "with", "IL-1beta", ",", "here", "used", "as", "positive", "control", "(", "195+/-20", "cells/mm2", ")", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "number", "of", "rolling", "cells", "on", "endothelial", "surface", "was", "not", "affected", "by", "high", "glucose", "level", "."]} {"tags": [0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0], "tokens": ["Stable", "adhesion", "of", "leukocytes", "to", "glucose-treated", "as", "well", "as", "to", "IL-1beta-stimulated", "endothelial", "cells", "was", "preceded", "by", "short", "interaction", "of", "leukocytes", "with", "the", "endothelial", "surface", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "distance", "travelled", "by", "leukocytes", "before", "arrest", "on", "30", "mM", "glucose", ",", "or", "on", "IL-1beta-treated", "endothelial", "cells", ",", "was", "significantly", "(", "P", "<", "0.01", ")", "higher", "than", "that", "observed", "for", "leukocytes", "adhering", "on", "control", "endothelium", "(", "30", "mM", "glucose", ":", "76.7+/-3.5", ";", "IL1beta", ":", "69.7+/-4", "versus", "5", "mM", "glucose", ":", "21.5+/-5", "microm", ")", "."]} {"tags": [0, 0, 0, 3, 0, 3, 4, 4, 4, 0, 0, 3, 4, 4, 4, 0, 5, 6, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Functional", "blocking", "of", "E-selectin", ",", "intercellular", "cell", "adhesion", "molecule-1", ",", "and", "vascular", "cell", "adhesion", "molecule-1", "on", "endothelial", "cells", "with", "the", "corresponding", "mouse", "mAb", "significantly", "inhibited", "glucose-induced", "increase", "in", "leukocyte", "adhesion", "(", "67+/-16", ",", "83+/-12", ",", "62+/-8", "versus", "144+/-21", "cells/", "mm2", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 4, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Confocal", "fluorescence", "microscopy", "studies", "showed", "that", "30", "mM", "glucose", "induced", "an", "increase", "in", "endothelial", "surface", "expression", "of", "E-selectin", ",", "intercellular", "cell", "adhesion", "molecule-1", ",", "and", "vascular", "cell", "adhesion", "molecule-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Electrophoretic", "mobility", "shift", "assay", "of", "nuclear", "extracts", "of", "human", "umbilical", "vein", "endothelial", "cells", "(", "HUVEC", ")", "exposed", "for", "1", "h", "to", "30", "mM", "glucose", "revealed", "an", "intense", "NF-kB", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Treatment", "of", "HUVEC", "exposed", "to", "high", "glucose", "with", "the", "NF-kB", "inhibitors", "pyrrolidinedithiocarbamate", "(", "100", "microM", ")", "and", "tosyl-phe-chloromethylketone", "(", "25", "microM", ")", "significantly", "reduced", "(", "P", "<", "0.05", ")", "leukocyte", "adhesion", "in", "respect", "to", "HUVEC", "treated", "with", "glucose", "alone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "significant", "(", "P", "<", "0.01", ")", "inhibitory", "effect", "on", "glucose-induced", "leukocyte", "adhesion", "was", "observed", "after", "blocking", "protein", "kinase", "C", "activity", "with", "staurosporine", "(", "5", "nM", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["When", "HUVEC", "were", "treated", "with", "specific", "antisense", "oligodesoxynucleotides", "against", "PKCalpha", "and", "PKCepsilon", "isoforms", "before", "the", "addition", "of", "30", "mM", "glucose", ",", "a", "significant", "(", "P", "<", "0.05", ")", "reduction", "in", "the", "adhesion", "was", "also", "seen", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Advanced", "glycosylation", "end", "product-albumin", "significantly", "increased", "the", "number", "of", "adhering", "leukocytes", "in", "respect", "to", "native", "albumin", "used", "as", "control", "(", "110+/-16", "versus", "66+/-7", ",", "P", "<", "0.01", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0], "tokens": ["Sera", "from", "diabetic", "patients", "significantly", "(", "P", "<", "0.01", ")", "enhanced", "leukocyte", "adhesion", "as", "compared", "with", "controls", ",", "despite", "normal", "levels", "of", "IL-1beta", "and", "TNFalpha", "in", "these", "sera", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["These", "data", "indicate", "that", "high", "glucose", "concentration", "and", "hyperglycemia", "promote", "leukocyte", "adhesion", "to", "the", "endothelium", "through", "upregulation", "of", "cell", "surface", "expression", "of", "adhesive", "proteins", ",", "possibly", "depending", "on", "NF-kB", "activation", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ikaros", "in", "hemopoietic", "lineage", "determination", "and", "homeostasis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Studies", "on", "the", "molecular", "mechanisms", "that", "control", "hemopoietic", "differentiation", "have", "focused", "on", "signaling", "cascades", "and", "nuclear", "effectors", "that", "drive", "this", "complex", "developmental", "system", "in", "a", "regulated", "fashion", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "review", "the", "role", "of", "Ikaros", ",", "the", "founding", "member", "of", "a", "unique", "family", "of", "zinc", "finger", "transcription", "factors", "in", "this", "developmental", "process", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Studies", "on", "an", "Ikaros", "null", "mutation", "have", "revealed", "an", "essential", "role", "for", "this", "factor", "in", "lymphoid", "cell", "fate", "determination", "and", "at", "subsequent", "branch", "points", "of", "the", "T", "cell", "differentiation", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Differences", "in", "the", "phenotypes", "of", "a", "null", "and", "a", "dominant", "negative", "(", "DN", ")", "Ikaros", "mutation", "provide", "insight", "into", "a", "regulatory", "network", "through", "which", "Ikaros", "proteins", "exert", "their", "effects", "in", "development", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 3], "tokens": ["In", "addition", "a", "comparative", "analysis", "of", "the", "hemopoietic", "stem", "cell", "and", "precursor", "compartment", "resulting", "from", "the", "two", "Ikaros", "mutations", "reveals", "a", "profound", "yet", "not", "absolute", "requirement", "for", "Ikaros"]} {"tags": [0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 1, 2, 2, 2, 0, 0, 3, 4, 4, 4, 3, 0], "tokens": ["Induction", "of", "early", "B", "cell", "factor", "(", "EBF", ")", "and", "multiple", "B", "lineage", "genes", "by", "the", "basic", "helix-loop-helix", "transcription", "factor", "E12", "."]} {"tags": [0, 3, 4, 0, 0, 0, 3, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "transcription", "factors", "encoded", "by", "the", "E2A", "and", "early", "B", "cell", "factor", "(", "EBF", ")", "genes", "are", "required", "for", "the", "proper", "development", "of", "B", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "absence", "of", "B", "lineage", "cells", "in", "E2A-", "and", "EBF-deficient", "mice", "has", "made", "it", "difficult", "to", "determine", "the", "function", "or", "relationship", "between", "these", "proteins", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "report", "the", "identification", "of", "a", "novel", "model", "system", "in", "which", "the", "role", "of", "E2A", "and", "EBF", "in", "the", "regulation", "of", "multiple", "B", "lineage", "traits", "can", "be", "studied", "."]} {"tags": [0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["We", "found", "that", "the", "conversion", "of", "70Z/3", "pre-B", "lymphocytes", "to", "cells", "with", "a", "macrophage-like", "phenotype", "is", "associated", "with", "the", "loss", "of", "E2A", "and", "EBF", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 7, 8, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "we", "show", "that", "ectopic", "expression", "of", "the", "E2A", "protein", "E12", "in", "this", "macrophage", "line", "results", "in", "the", "induction", "of", "many", "B", "lineage", "genes", ",", "including", "EBF", ",", "IL7Ralpha", ",", "lambda5", ",", "and", "Rag-1", ",", "and", "the", "ability", "to", "induce", "kappa", "light", "chain", "in", "response", "to", "mitogen", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Activation", "of", "EBF", "may", "be", "one", "of", "the", "critical", "functions", "of", "E12", "in", "regulating", "the", "B", "lineage", "phenotype", "since", "expression", "of", "EBF", "alone", "leads", "to", "the", "activation", "of", "a", "subset", "of", "E12", "-inducible", "traits", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 3, 0, 0, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Our", "data", "demonstrate", "that", ",", "in", "the", "context", "of", "this", "macrophage", "line", ",", "E12", "induces", "expression", "of", "EBF", "and", "together", "these", "transcription", "factors", "coordinately", "regulate", "numerous", "B", "lineage-associated", "genes", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 5, 6, 6, 0], "tokens": ["Activation-induced", "down-regulation", "of", "retinoid", "receptor", "RXRalpha", "expression", "in", "human", "T", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0], "tokens": ["Role", "of", "cell", "cycle", "regulation", "."]} {"tags": [0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "5.4-kilobase", "mRNA", ",", "the", "expression", "of", "which", "is", "down-regulated", "after", "treatment", "of", "human", "peripheral", "blood", "mononuclear", "cells", "(", "PBMCs", ")", "with", "various", "T", "cell-activating", "agents", ",", "was", "isolated", "using", "an", "mRNA", "differential", "display", "method", "."]} {"tags": [0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 9, 0, 9, 10, 10, 10, 10, 0], "tokens": ["Nucleotide", "sequence", "analysis", "identified", "the", "5", "'", "end", "of", "this", "RNA", "as", "human", "retinoid", "receptor", "RXRalpha", "mRNA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", ",", "we", "report", "the", "nucleotide", "sequence", "of", "3.6", "kilobases", "of", "this", "RNA", ",", "which", "represents", "the", "3", "'", "end", "of", "RXRalpha", "mRNA", ",", "the", "sequence", "of", "which", "has", "not", "been", "previously", "described", "."]} {"tags": [0, 5, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Activated", "PBMCs", "also", "expressed", "lower", "levels", "of", "RXRalpha", "protein", ",", "and", "a", "DNA", "binding", "assay", "showed", "that", "the", "activation-induced", "loss", "of", "RXRalpha", "mRNA", "and", "protein", "expression", "correlated", "with", "the", "loss", "of", "DNA", "binding", "activity", "of", "this", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "present", "evidence", "that", "the", "transition", "from", "G0/G1", "to", "S", "phase", "of", "the", "cell", "cycle", "results", "in", "the", "down-regulation", "of", "RXRalpha", "expression", "and", "that", "cell", "cycle", "inhibitors", ",", "which", "block", "the", "cells", "in", "G1", "phase", ",", "prevent", "this", "down-regulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "decrease", "in", "the", "levels", "of", "RXRalpha", "mRNA", "was", "found", "to", "be", "regulated", "at", "the", "post-transcriptional", "level", "and", "involved", "new", "protein", "synthesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "observations", "indicate", "that", "the", "levels", "of", "RXRalpha", "expression", "in", "T", "lymphocytes", "are", "coupled", "to", "cell", "cycle", "progression", ",", "and", "there", "is", "tight", "regulatory", "control", "of", "RXRalpha", "expression", "during", "the", "transition", "from", "G0/G1", "to", "S", "phase", "of", "the", "cell", "cycle", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Epidemiology", "and", "pathogenesis", "of", "AIDS-related", "lymphomas", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Among", "patients", "with", "congenital", "and", "acquired", "immunodeficiencies", ",", "non-Hodgkin", "'s", "lymphoma", "(", "NHLs", ")", "are", "the", "most", "common", "tumors", "of", "the", "immune", "system", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "the", "setting", "of", "human", "immunodeficiency", "virus", "(", "HIV", ")", "infection", ",", "as", "many", "as", "10", "%", "to", "20", "%", "of", "people", "ultimately", "developed", "NHLs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "tumors", "are", "clinically", "aggressive", ",", "frequently", "involve", "extranodal", "sites", ",", "and", "often", "exhibit", "unique", "features", "that", "distinguish", "them", "from", "NHL", "arising", "in", "individuals", "with", "other", "forms", "of", "immunosuppression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 0, 1, 2, 0, 0, 0, 0], "tokens": ["Important", "in", "the", "development", "of", "HIV-associated", "NHL", "are", "cytokines", "and", "other", "factors", "that", "induce", "B-cell", "proliferation", "and", "increase", "the", "likelihood", "of", "mutations", "of", "c-myc", ",", "bcl-6", ",", "and", "other", "tumor-suppressor", "genes", "with", "carcinogenic", "potential", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Specific", "forms", "of", "HIV-associated", "NHL", "are", "linked", "to", "expression", "of", "Epstein-Barr", "virus", "(", "EBV", ")", "-latent", "proteins", ";", "the", "newly", "described", "DNA", "virus", ",", "Karposi", "'s", "sarcoma-associated", "herpesvirus/human", "herpesvirus-8", "(", "KSHV/HHV-8", ")", ";", "and", "perhaps", "HIV", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Elucidation", "of", "the", "factors", "that", "contribute", "to", "the", "high", "incidence", "of", "NHL", "among", "patients", "infected", "with", "HIV", "provides", "insights", "into", "important", "elements", "of", "lymphomagenesis", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 4, 0, 3, 0, 3, 0, 3, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Matrix", "metalloproteinase", "expression", "in", "human", "breast", "cancer", ":", "an", "immunohistochemical", "study", "including", "correlation", "with", "cathepsin", "D", ",", "type", "IV", "collagen", ",", "laminin", ",", "fibronectin", ",", "EGFR", ",", "c-erbB-2", "oncoprotein", ",", "p53", ",", "steroid", "receptors", "status", "and", "proliferative", "indices", "."]} {"tags": [3, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Matrix", "metalloproteinase", "s", "(", "MMPs", ")", "are", "a", "group", "of", "enzymes", "thought", "to", "be", "responsible", "for", "both", "normal", "connective", "tissue", "matrix", "remodelling", "and", "accelerated", "breakdown", "associated", "with", "tumour", "development", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 4, 4, 0, 3, 0, 0, 3, 0, 3, 4, 0, 3, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "current", "study", "aimed", "to", "investigate", "the", "immunohistochemical", "expression", "of", "matrix", "metalloproteinase", "3", "(", "MMP-3", ",", "stromelysin-1", ")", "in", "correlation", "with", "the", "expression", "of", "Basement", "Membrane", "(", "BM", ")", "antigen", "(", "type", "IV", "collagen", ",", "laminin", ")", ",", "fibronectin", ",", "cathepsin", "D", ",", "p53", ",", "c-erbB-2", ",", "proliferative", "activity", "(", "Ki-67", ",", "PCNA", ")", ",", "steroid", "receptor", "content", "as", "well", "as", "to", "the", "other", "conventional", "clinicopathological", "parameters", "in", "breast", "cancer", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0], "tokens": ["This", "study", "was", "performed", "on", "a", "series", "of", "frozen", "and", "paraffin", "sections", "from", "84", "breast", "cancer", "specimens", "by", "immunohistochemistry", "using", "the", "monoclonal", "antibody", "MMP-3", "(", "Ab-1", ")", "."]} {"tags": [3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Stromelysin-1", "(", "ST1", ")", "was", "observed", "in", "about", "10", "%", "of", "epithelial", "cells", "in", "the", "control", "groups", "(", "cases", "of", "fibrocystic", "and", "benign", "proliferative", "breast", "disease", ")", ",", "while", "expression", "(", ">", "10", "%", "of", "expression", ")", "was", "detected", "in", "89.7", "%", "of", "tumours", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "expression", "of", "ST1", "in", "carcinoma", "cells", "was", "strongly", "associated", "with", "its", "presence", "in", "the", "stroma", "(", "p", "<", "0.001", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "significantly", "positive", "correlation", "was", "found", "between", "ST1", "expression", ",", "and", "p53", "tumour", "suppressor", "gene", "product", "(", "p", "=", "0.004", ")", ",", "and", "a", "relationship", "with", "c-erbB-2", "protein", "and", "progesterone", "receptor", "status", "was", "also", "indicated", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "findings", "suggest", "that", "ST1", "expression", "in", "breast", "cancer", "tissue", "is", "irrespective", "of", "the", "expression", "of", "the", "extracellular", "matrix", "component", ",", "the", "proteolytic", "enzyme", "cathepsin", "D", "and", "the", "growth", "fraction", "of", "the", "tumour", ",", "and", "that", "it", "could", "be", "a", "potential", "new", "prognostic", "marker", "in", "breast", "cancer", "."]} {"tags": [0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Use", "of", "transfected", "liver", "cells", "to", "evaluate", "potential", "mechanisms", "of", "alcohol-induced", "liver", "injury", "[", "see", "comments", "]"]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "is", "increased", "activity", "of", "the", "proinflammatory", "cytokine", ",", "tumor", "necrosis", "factor", "(", "TNF", ")", "in", "alcoholic", "liver", "disease", "(", "ALD", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Hepatic", "neutrophil", "infiltration", "is", "a", "principal", "injurious", "manifestation", "of", "ALD", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["TNF", "can", "induce", "cellular", "oxidative", "injury", "directly", ",", "and", "indirectly", "by", "inducing", "neutrophil", "chemotactic", "factor", "(", "IL-8", ")", "production", "by", "hepatocytes", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["IL-8", "activates", "and", "chemotactically", "attracts", "neutrophils", "to", "the", "liver", "where", "they", "release", "oxidizing", "substances", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Patients", "with", "ALD", "also", "have", "decreased", "protective", "factors", "for", "cellular", "oxidative", "injury", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Manganous", "superoxide", "dismutase", "(", "MnSOD", ")", "is", "an", "antioxidant", "protective", "factor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 7, 8, 8, 8, 8, 0], "tokens": ["The", "objectives", "of", "these", "studies", "were", "to", "investigate", "mechanisms", "for", "induction", "of", "an", "injurious", "factor", "(", "IL-8", ")", "and", "a", "protective", "factor", "(", "MnSOD", ")", "in", "the", "HepG2", "human", "hepatoma", "cell", "line", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 7, 8, 8, 0, 0, 3, 0, 0, 0, 0], "tokens": ["In", "the", "first", "set", "of", "experiments", ",", "IL-8", "gene", "reporter", "constructs", "were", "used", "to", "transiently", "transfect", "a", "derivative", "(", "MVh2E1-9", ")", "of", "the", "HepG2", "cell", "line", "which", "expresses", "P-4502E1", "and", "metabolizes", "ethanol", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 1, 2, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 1, 2, 2, 0, 1, 2, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Inactivation", "of", "the", "NF-kappaB", "and", "3'NF-IL-6", "DNA", "binding", "sites", "decreased", "IL-8", "gene", "transcriptional", "activation", "in", "response", "to", "TNF", "while", "inactivation", "of", "the", "5'NF-IL-6", "binding", "site", "increased", "IL-8", "gene", "transcriptional", "activity", "in", "response", "to", "TNF", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0], "tokens": ["This", "system", "may", "be", "useful", "to", "assess", "the", "effects", "of", "ethanol", "on", "TNF", "-induced", "hepatocyte", "IL-8", "production", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "the", "second", "set", "of", "experiments", ",", "HepG2", "cells", "were", "cultured", "in", "25", "to", "100", "mmol", "concentrations", "of", "ethanol", "."]} {"tags": [0, 3, 0, 0, 0, 7, 8, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Both", "TNF", "and", "ethanol", "increased", "HepG2", "cell", "MnSOD", "activity", "in", "short-term", "(", "72", "hr", ")", "cultures", "with", "ethanol", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0], "tokens": ["However", ",", "after", "long-term", "(", "10", "weeks", ")", "culture", "with", "ethanol", ",", "there", "was", "no", "induction", "of", "MnSOD", "by", "ethanol", "and", "there", "was", "a", "diminished", "induction", "of", "MnSOD", "in", "response", "to", "TNF", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 7, 8, 0, 0, 7, 8, 0], "tokens": ["Further", "studies", "are", "needed", "to", "assess", "the", "effect", "of", "this", "diminished", "induction", "of", "MnSOD", "with", "chronic", "ethanol", "culture", "on", "HepG2", "cell", "susceptibility", "to", "TNF", "cytotoxicity", "."]} {"tags": [0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["We", "conclude", "that", "transfected", "liver", "cell", "lines", "can", "be", "used", "to", "evaluate", "mechanisms", "for", "increased", "injurious", "factors", "and", "decreased", "protective", "factors", "in", "alcoholic", "liver", "injury", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["Tissue", "factor", "transcription", "driven", "by", "Egr-1", "is", "a", "critical", "mechanism", "of", "murine", "pulmonary", "fibrin", "deposition", "in", "hypoxia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Local", "hypoxemia", "and", "stasis", "trigger", "thrombosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0], "tokens": ["We", "have", "demonstrated", "previously", "that", "in", "a", "murine", "model", "of", "normobaric", "hypoxia", "pulmonary", "fibrin", "deposition", "is", "a", "result", "of", "expression", "of", "tissue", "factor", ",", "especially", "in", "oxygen-deprived", "mononuclear", "phagocytes", "(", "MPs", ")", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["We", "now", "show", "that", "transcription", "factor", "early-growth-response", "gene", "product", "(", "Egr-1", ")", "is", "rapidly", "activated", "in", "hypoxia", ",", "both", "in", "vitro", "and", "in", "vivo", ",", "and", "is", "responsible", "for", "transcription", "and", "expression", "of", "tissue", "factor", "in", "hypoxic", "lung", "."]} {"tags": [3, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["MPs", "and", "HeLa", "cells", "subjected", "to", "hypoxia", "(", "pO2", "approximately", "13", "torr", ")", "had", "increased", "levels", "of", "tissue", "factor", "transcripts", "(", "approximately", "18-fold", ")", "and", "an", "increased", "rate", "of", "transcription", "(", "approximately", "15-fold", ")", ",", "based", "on", "nuclear", "run-on", "analysis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 7, 8, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 2, 0], "tokens": ["Gel-shift", "analysis", "of", "nuclear", "extracts", "from", "hypoxic", "MPs", "and", "HeLa", "cells", "demonstrated", "increased", "DNA-binding", "activity", "at", "the", "serum", "response", "region", "(", "SRR", ";", "-111/+14", "bp", ")", "of", "the", "tissue", "factor", "promoter", "at", "Egr-1", "motifs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "32P-labeled", "Egr", "consensus", "oligonucleotide", ",", "we", "observed", "induction", "of", "DNA-binding", "activity", "in", "nuclear", "extracts", "from", "hypoxic", "lung", "and", "HeLa", "cells", "because", "of", "activation", "of", "Egr-1", ",", "by", "means", "of", "supershift", "analysis", "."]} {"tags": [0, 0, 0, 7, 8, 0, 1, 2, 0, 3, 4, 4, 4, 0, 0, 1, 2, 2, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0], "tokens": ["Transient", "transfection", "of", "HeLa", "cells", "with", "chimeric", "plasmids", "containing", "wild-type", "or", "mutant", "SRR", "from", "the", "tissue", "factor", "promoter", "showed", "that", "intact", "Sp1", "sites", "are", "necessary", "for", "basal", "promoter", "activity", ",", "whereas", "the", "integrity", "of", "Egr-1", "sites", "was", "required", "for", "hypoxia-enhanced", "expression", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 3, 0], "tokens": ["A", "central", "role", "for", "Egr-1", "in", "hypoxia-mediated", "tissue", "factor", "expression", "was", "confirmed", "by", "experiments", "with", "homozygous", "Egr-1", "null", "mice", ";", "wild-type", "mice", "subjected", "to", "oxygen", "deprivation", "expressed", "tissue", "factor", "and", "showed", "fibrin", "deposition", ",", "but", "hypoxic", "homozygous", "Egr-1", "null", "mice", "displayed", "neither", "tissue", "factor", "nor", "fibrin", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "delineate", "a", "novel", "biology", "for", "hypoxia-induced", "fibrin", "deposition", ",", "in", "which", "oxygen", "deprivation-induced", "activation", "of", "Egr-1", ",", "resulting", "in", "expression", "of", "tissue", "factor", ",", "has", "an", "unexpected", "and", "central", "role", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 3, 4, 0], "tokens": ["Kinetics", "of", "cytokine", "and", "NFAT", "gene", "expression", "in", "human", "interleukin-2-dependent", "T", "lymphoblasts", "stimulated", "via", "T-cell", "receptor", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T", "cells", "respond", "to", "mitogenic", "or", "antigenic", "stimulation", "by", "proliferation", "and", "by", "turning", "on", "cytokine", "gene", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 3, 4, 0, 3, 4, 4, 0, 3, 0, 0], "tokens": ["Here", "we", "have", "analysed", "the", "kinetics", "and", "nature", "of", "cytokine", "production", "in", "human", "peripheral", "blood-derived", "T", "lymphoblasts", "stimulated", "with", "anti-CD3", "antibodies", "or", "Lens", "culinaris", "lectin", "(", "LCL", ")", "."]} {"tags": [5, 6, 0, 0, 0, 5, 6, 6, 6, 0, 5, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0], "tokens": ["T", "cells", "were", "purified", "from", "peripheral", "blood", "mononuclear", "cells", "(", "PBMC", ")", "and", "primarily", "activated", "with", "anti-CD3", "antibodies", "and", "cultured", "in", "the", "presence", "of", "interleukin-2", "(", "IL-2", ")", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0], "tokens": ["Anti-CD3-restimulated", "T", "cells", "(", "mainly", "CD8+", ")", "produced", "IL-2", ",", "interferon-gamma", "(", "IFN-gamma", ")", "and", "tumour", "necrosis", "factor-alpha", "(", "TNF-alpha", ")", "and", "low", "levels", "of", "IL-4", "and", "IL-10", "transcripts", "and", "proteins", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0], "tokens": ["No", "IL-6", "gene", "expression", "was", "observed", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "LCL-stimulated", "cells", "the", "cytokine", "production", "pattern", "was", "very", "similar", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Steady-state", "mRNA", "levels", "of", "IL-2", ",", "IL-10", "and", "IFN-gamma", "peaked", "at", "3", "hr", "after", "anti-CD3", "stimulation", "and", "declined", "rapidly", "thereafter", "."]} {"tags": [0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "kinetics", "of", "TNF-alpha", "mRNA", "expression", "was", "faster", ",", "being", "at", "its", "peak", "level", "1", "hr", "after", "stimulation", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Anti-CD3-stimulated", "IL-2", "gene", "expression", "was", "down-regulated", "by", "protein", "synthesis", "inhibitor", ",", "whereas", "IL-10", ",", "IFN-gamma", "and", "TNF-alpha", "genes", "were", "readily", "induced", "independent", "of", "ongoing", "protein", "synthesis", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T-cell", "receptor", "stimulation", "also", "induced", "a", "very", "rapid", "expression", "of", "c-jun", ",", "c-fos", "and", "NFATc1", "(", "NFATc", ")", "genes", ",", "the", "gene", "products", "of", "which", "are", "involved", "in", "cytokine", "gene", "expression", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 5, 6, 6, 0, 0, 3, 0, 3, 0, 3, 0], "tokens": ["In", "conclusion", ",", "the", "cytokines", "synthesized", "by", "IL-2-dependent", "T", "cells", "were", "predominantly", "IL-2", ",", "IFN-gamma", "and", "TNF-alpha", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["An", "animal", "model", "to", "study", "local", "oxidation", "of", "LDL", "and", "its", "biological", "effects", "in", "the", "arterial", "wall", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Oxidized", "LDL", "(", "oxLDL", ")", "is", "present", "in", "atherosclerotic", "lesions", "and", "is", "believed", "to", "play", "a", "key", "role", "in", "atherogenesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mainly", "on", "the", "basis", "of", "cell", "culture", "studies", ",", "oxLDL", "has", "been", "shown", "to", "produce", "many", "biological", "effects", "that", "influence", "the", "atherosclerotic", "process", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "study", "LDL", "oxidation", "in", "vivo", ",", "we", "have", "established", "a", "model", "in", "which", "Sprague-Dawley", "rats", "are", "given", "a", "single", "injection", "of", "unmodified", "human", "LDL", "(", ">", "or", "=", "4", "mg/kg", "body", "weight", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Within", "6", "hours", ",", "an", "accumulation", "of", "apolipoprotein", "B", "and", "epitopes", "present", "on", "oxLDL", "are", "detected", "in", "the", "arterial", "endothelium", "and", "media", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "presence", "of", "oxLDL", "is", "associated", "with", "activation", "of", "the", "transcription", "factor", "nuclear", "factor-kappaB", "in", "the", "endothelium", "as", "well", "as", "endothelial", "expression", "of", "intercellular", "adhesion", "molecule-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Injection", "of", "LDL", "enriched", "with", "the", "antioxidant", "probucol", "resulted", "in", "arterial", "accumulation", "of", "apolipoprotein", "B", ",", "but", "the", "expression", "of", "oxLDL-specific", "epitopes", "was", "reduced", "at", "24", "hours", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "this", "simple", "model", "has", "the", "potential", "to", "analyze", "the", "mechanisms", "behind", "and", "biological", "effects", "of", "LDL", "oxidation", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Molecular", "mechanisms", "of", "promoter", "regulation", "of", "the", "gp34", "gene", "that", "is", "trans-activated", "by", "an", "oncoprotein", "Tax", "of", "human", "T", "cell", "leukemia", "virus", "type", "I", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "investigated", "the", "molecular", "mechanism", "of", "transcriptional", "activation", "of", "the", "gp34", "gene", "by", "the", "Tax", "oncoprotein", "of", "human", "T", "cell", "leukemia", "virus", "type", "I", "(", "HTLV-I", ")", "."]} {"tags": [3, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 5, 6, 6, 6, 0], "tokens": ["gp34", "is", "a", "type", "II", "transmembrane", "molecule", "belonging", "to", "the", "tumor", "necrosis", "factor", "family", "and", "is", "constitutively", "expressed", "on", "HTLV-I-producing", "cells", "but", "not", "normal", "resting", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 3, 0, 0, 7, 8, 8, 8, 8, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0], "tokens": ["The", "transcriptional", "regulatory", "region", "of", "the", "gp34", "gene", "was", "activated", "by", "HTLV-I", "Tax", "in", "the", "human", "T", "cell", "line", "Jurkat", ",", "in", "which", "endogenous", "gp34", "is", "induced", "by", "Tax", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Sequence", "analysis", "demonstrated", "that", "two", "NF-kappaB-like", "elements", "(", "1", "and", "2", ")", "were", "present", "in", "the", "regulatory", "region", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Both", "NF-kappaB-like", "elements", "were", "able", "to", "bind", "to", "NF-kappaB", "or", "its", "related", "factor", "(", "s", ")", "in", "a", "Tax", "-dependent", "manner", "."]} {"tags": [3, 4, 0, 0, 0, 1, 2, 2, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Chloramphenicol", "acetyltransferase", "assays", "indicated", "that", "NF-kappaB-like", "element", "1", "was", "Tax", "-responsive", ",", "although", "the", "activity", "was", "lower", "than", "that", "the", "native", "promoter", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NF-kappaB", "-like", "element", "2", "elevated", "promoter", "activity", "when", "combined", "with", "NF-kappaB-like", "element", "1", ",", "indicating", "cooperative", "function", "of", "the", "elements", "for", "maximum", "promoter", "function", "."]} {"tags": [0, 0, 1, 2, 0, 0, 1, 2, 0, 3, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Unlike", "typical", "NF-kappaB", "elements", ",", "the", "NF-kappaB-like", "elements", "in", "gp34", "were", "not", "activated", "by", "treatment", "of", "Jurkat", "cells", "with", "phorbol", "ester", "despite", "induction", "of", "the", "NF-kappaB", "-like", "binding", "activity", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0], "tokens": ["Chloramphenicol", "acetyltransferase", "reporter", "assays", "using", "the", "region", "upstream", "of", "the", "NF-kappaB-like", "elements", "identified", "an", "upstream", "region", "that", "reduced", "transcription", "from", "cognate", "and", "noncognate", "core", "promoters", "in", "a", "Tax-independent", "manner", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "results", "imply", "complex", "regulation", "of", "expression", "of", "the", "gp34", "gene", "and", "suggest", "implication", "of", "gp34", "in", "proliferation", "of", "HTLV-I", "infected", "T", "cells", "."]} {"tags": [3, 4, 0, 0, 3, 4, 4, 0, 0, 5, 0, 7, 8, 0], "tokens": ["beta-Amyloid", "fibrils", "activate", "parallel", "mitogen-activated", "protein", "kinase", "pathways", "in", "microglia", "and", "THP1", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["The", "senile", "plaques", "of", "Alzheimer", "'s", "disease", "are", "foci", "of", "local", "inflammatory", "responses", ",", "as", "evidenced", "by", "the", "presence", "of", "acute", "phase", "proteins", "and", "oxidative", "damage", "."]} {"tags": [3, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["Fibrillar", "forms", "of", "beta-amyloid", "(", "Abeta", ")", ",", "which", "are", "the", "primary", "constituents", "of", "senile", "plaques", ",", "have", "been", "shown", "to", "activate", "tyrosine", "kinase-dependent", "signal", "transduction", "cascades", ",", "resulting", "in", "inflammatory", "responses", "in", "microglia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "downstream", "signaling", "pathways", "mediating", "Abeta", "-induced", "inflammatory", "events", "are", "not", "well", "characterized", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 0, 7, 8, 8, 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0], "tokens": ["We", "report", "that", "exposure", "of", "primary", "rat", "microglia", "and", "human", "THP1", "monocytes", "to", "fibrillar", "Abeta", "results", "in", "the", "tyrosine", "kinase", "-dependent", "activation", "of", "two", "parallel", "signal", "transduction", "cascades", "involving", "members", "of", "the", "mitogen-activated", "protein", "kinase", "(", "MAPK", ")", "superfamily", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 0, 5, 0, 3, 0, 7, 8, 0], "tokens": ["Abeta", "stimulated", "the", "rapid", ",", "transient", "activation", "of", "extracellular", "signal-regulated", "kinase", "1", "(", "ERK1", ")", "and", "ERK2", "in", "microglia", "and", "ERK2", "in", "THP1", "monocytes", "."]} {"tags": [0, 0, 3, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "second", "superfamily", "member", ",", "p38", "MAPK", ",", "was", "also", "activated", "with", "similar", "kinetics", "."]} {"tags": [3, 4, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Scavenger", "receptor", "and", "receptor", "for", "advanced", "glycated", "end", "products", "(", "RAGE", ")", "ligands", "failed", "to", "activate", "ERK", "and", "p38", "MAPK", "in", "the", "absence", "of", "significant", "increases", "in", "protein", "tyrosine", "phosphorylation", ",", "demonstrating", "that", "scavenger", "receptors", "and", "RAGE", "are", "not", "linked", "to", "these", "pathways", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Importantly", ",", "the", "stress-activated", "protein", "kinases", "(", "SAPKs", ")", "were", "not", "significantly", "activated", "in", "response", "to", "Abeta", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Downstream", "effectors", "of", "the", "MAPK", "signal", "transduction", "cascades", "include", "MAPKAP", "kinases", ",", "such", "as", "RSK1", "and", "RSK2", ",", "as", "well", "as", "transcription", "factors", "."]} {"tags": [0, 0, 5, 0, 7, 8, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Exposure", "of", "microglia", "and", "THP1", "monocytes", "to", "Abeta", "resulted", "in", "the", "activation", "of", "RSK1", "and", "RSK2", "and", "phosphorylation", "of", "cAMP", "response", "element-binding", "protein", "at", "Ser133", ",", "providing", "a", "mechanism", "for", "Abeta", "-induced", "changes", "in", "gene", "expression"]} {"tags": [0, 0, 3, 4, 4, 4, 4, 0, 3, 4, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Regulation", "of", "cellular", "retinoic", "acid", "binding", "protein", "(", "CRABP", "II", ")", "during", "human", "monocyte", "differentiation", "in", "vitro", "."]} {"tags": [3, 4, 4, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Cellular", "retinoic", "acid", "binding", "proteins", "(", "CRABP", ")", "are", "low", "molecular", "weight", "proteins", "whose", "precise", "function", "remains", "unknown", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["They", "bind", "retinoids", "and", "may", "thereby", "modulate", "the", "intracellular", "steady-state", "concentration", "of", "retinoids", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Whereas", "CRABP", "I", "is", "ubiquitously", "expressed", ",", "CRABP", "II", "is", "mainly", "detected", "in", "various", "cell", "types", "of", "the", "skin", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 5, 6, 6, 0, 5, 0, 0, 0, 0, 5, 6, 6, 0, 5, 0, 0], "tokens": ["By", "representative", "difference", "analysis", "we", "found", "that", "CRABP", "II", "is", "also", "strongly", "expressed", "in", "human", "monocyte-derived", "macrophages", "(", "MAC", ")", "but", "not", "in", "freshly", "isolated", "monocytes", "(", "MO", ")", "."]} {"tags": [0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 5, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "CRABP", "II", "mRNA", "was", "gradually", "upregulated", "during", "differentiation", "from", "MO", "to", "MAC", "in", "the", "presence", "of", "2", "%", "serum", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Adherence", ",", "which", "is", "important", "for", "MO", "differentiation", ",", "induced", "CRABP", "II", "expression", ",", "but", "the", "addition", "of", "10", "(", "-7", ")", "M", "retinoic", "acid", "inhibited", "the", "upregulation", "of", "CRABP", "II", "expression", "during", "MO/MAC", "differentiation", "."]} {"tags": [0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0], "tokens": ["As", "MO", "can", "differentiate", "along", "the", "classical", "pathway", "not", "only", "to", "MAC", "but", "also", "to", "dendritic", "cells", "we", "analyzed", "the", "expression", "of", "CRABP", "II", "in", "MO-derived", "dendritic", "cells", "cultured", "with", "10", "%", "FCS", ",", "IL-4", ",", "and", "GM-CSF", "."]} {"tags": [0, 0, 0, 5, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["In", "contrast", "to", "MAC", ",", "MO-derived", "dendritic", "cells", "showed", "an", "extremely", "low", "expression", "of", "CRABP", "II", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 5, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["From", "these", "results", "we", "conclude", "(", "1", ")", "that", "the", "availability", "and", "the", "metabolism", "of", "retinoids", "may", "be", "different", "in", "MAC", "compared", "to", "MO", "and", "dendritic", "cells", "and", "(", "2", ")", "that", "this", "may", "influence", "differentiation", "and", "activation", "of", "those", "cells", "."]} {"tags": [3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transcription", "factor", "B-cell-specific", "activator", "protein", "(", "BSAP", ")", "is", "differentially", "expressed", "in", "B", "cells", "and", "in", "subsets", "of", "B-cell", "lymphomas", "."]} {"tags": [0, 1, 2, 2, 2, 1, 0, 0, 3, 4, 3, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "paired", "box", "containing", "gene", "PAX-5", "encodes", "the", "transcription", "factor", "BSAP", "(", "B-cell-specific", "activator", "protein", ")", ",", "which", "plays", "a", "key", "role", "in", "B-lymphocyte", "development", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Despite", "its", "known", "involvement", "in", "a", "rare", "subtype", "of", "non-Hodgkin", "'s", "lymphoma", "(", "NHL", ")", ",", "a", "detailed", "examination", "of", "BSAP", "expression", "in", "NHL", "has", "not", "been", "previously", "reported", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", ",", "we", "analyzed", "normal", "and", "malignant", "lymphoid", "tissues", "and", "cell", "lines", ",", "including", "102", "cases", "of", "B-cell", "NHL", ",", "23", "cases", "of", "T-", "and", "null-cell", "NHL", ",", "and", "18", "cases", "of", "Hodgkin", "'s", "disease", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Normal", "lymphoid", "tissues", "showed", "strong", "nuclear", "BSAP", "expression", "in", "mantle", "zone", "B", "cells", ",", "less", "intense", "reactivity", "in", "follicular", "center", "B", "cells", ",", "and", "no", "expression", "in", "cells", "of", "the", "T-cell-rich", "zones", "."]} {"tags": [5, 6, 6, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 6, 6, 6, 0, 0, 0], "tokens": ["Monocytoid", "B", "cells", "showed", "weak", "expression", ",", "whereas", "plasma", "cells", "and", "extrafollicular", "large", "transformed", "B", "cells", "were", "negative", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Of", "the", "102", "B-cell", "NHLs", ",", "83", "(", "81", "%", ")", "demonstrated", "BSAP", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["All", "of", "the", "13", "(", "100", "%", ")", "B-cell", "chronic", "lymphocytic", "leukemias", "(", "B-CLLs", ")", ",", "21", "of", "(", "100", "%", ")", "mantle", "cells", "(", "MCLs", ")", ",", "and", "20", "of", "21", "(", "95", "%", ")", "follicular", "lymphomas", "(", "FLs", ")", "were", "positive", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Moderate", "staining", "intensities", "were", "found", "in", "most", "B-CLL", "and", "FL", "cases", ",", "whereas", "most", "MCLs", "showed", "strong", "reactions", ",", "paralleling", "the", "strong", "reactivity", "of", "nonmalignant", "mantle", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Eight", "of", "12", "(", "67", "%", ")", "marginal", "zone", "lymphoma", "cases", "showed", "negative", "or", "low", "BSAP", "levels", ",", "and", "17", "of", "24", "(", "71", "%", ")", "large", "B-cell", "lymphomas", "displayed", "moderate", "to", "strong", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["of", "the", "23", "T-", "and", "null-cell", "lymphomas", "reacted", "with", "the", "BSAP", "antisera", ",", "whereas", "in", "Hodgkin", "'s", "disease", ",", "2", "of", "4", "(", "50", "%", ")", "nodular", "lymphocytic", "predominance", "and", "5", "of", "14", "(", "36", "%", ")", "classical", "cases", "showed", "weak", "nuclear", "or", "nucleolar", "BSAP", "reactions", "in", "a", "fraction", "of", "the", "tumor", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 7, 8, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0], "tokens": ["Western", "blot", "analysis", "showed", "a", "52-kD", "BSAP", "band", "in", "B-cell", "lines", ",", "but", "not", "in", "non-B-cell", "or", "plasma", "cell", "lines", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["We", "conclude", "that", "BSAP", "expression", "is", "largely", "restricted", "to", "lymphomas", "of", "B-cell", "lineage", "and", "that", "BSAP", "expression", "varies", "in", "B-cell", "subsets", "and", "subtypes", "of", "B-cell", "NHL", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0], "tokens": ["The", "high", "levels", "of", "BSAP", ",", "especially", "those", "found", "in", "large-cell", "lymphomas", "and", "in", "some", "follicular", "lymphomas", ",", "may", "be", "a", "consequence", "of", "deregulated", "gene", "expression", "and", "suggest", "a", "possible", "involvement", "of", "PAX-5", "in", "certain", "B-cell", "malignancies", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "is", "a", "US", "government", "work", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "are", "no", "restrictions", "on", "its", "use", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 0], "tokens": ["Mitogen", "and", "growth", "factor-induced", "activation", "of", "a", "STAT-like", "molecule", "in", "channel", "catfish", "lymphoid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "article", "describes", "the", "identification", "of", "a", "putative", "STAT", "molecule", "in", "the", "channel", "catfish", "(", "Ictalurus", "punctatus", ")", ",", "the", "first", "report", "of", "such", "a", "molecule", "in", "a", "'lower", "'", "vertebrate", "."]} {"tags": [0, 3, 4, 0, 3, 4, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "monoclonal", "antibody", "against", "human", "STAT6", "recognizes", "an", "approximately", "100", "kDa", "molecule", "that", "becomes", "activated", "and", "translocates", "to", "the", "nucleus", "upon", "both", "growth", "factor", "and", "mitogen", "stimulation", "of", "catfish", "leukocytes", "."]} {"tags": [0, 0, 3, 4, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "presumed", "catfish", "STAT", "binds", "the", "mammalian", "interferon-gamma", "activation", "site", ",", "a", "known", "motif", "of", "mammalian", "STAT", "binding", ",", "as", "shown", "by", "electromobility", "shift", "assays", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["Purification", "of", "the", "proteins", "present", "in", "these", "DNA", "complexes", "confirms", "that", "the", "catfish", "reactive", "molecule", "binds", "to", "the", "interferon-gamma", "activation", "site", "sequence", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "suggest", "that", "STAT", "molecules", "have", "been", "highly", "conserved", "in", "vertebrate", "evolution", "."]} {"tags": [0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 3, 4, 4, 4, 4, 4, 0], "tokens": ["Isolation", "and", "analysis", "of", "a", "T", "cell", "clone", "variant", "exhibiting", "constitutively", "phosphorylated", "Ser133", "cAMP", "response", "element-binding", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["In", "driving", "T", "cell", "proliferation", ",", "IL-2", "stimulates", "a", "new", "program", "of", "gene", "expression", "that", "includes", "proliferating", "cell", "nuclear", "antigen", "(", "PCNA", ")", ",", "a", "requisite", "processivity", "factor", "for", "DNA", "polymerase", "delta", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 7, 0], "tokens": ["PCNA", "transcription", "is", "regulated", "in", "part", "through", "tandem", "CRE", "sequences", "in", "the", "promoter", "and", "CRE", "binding", "proteins", ";", "IL-2", "stimulates", "CREB", "phosphorylation", "in", "the", "resting", "cloned", "T", "lymphocyte", ",", "L2", "."]} {"tags": [0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["After", "culturing", "L2", "cells", "for", "greater", "than", "91", "days", ",", "we", "consistently", "isolate", "a", "stable", "variant", "that", "exhibits", "constitutive", "CREB", "phosphorylation", "."]} {"tags": [7, 8, 8, 8, 8, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["L2", "and", "L2", "variant", "cells", "were", "tested", "for", "IL-2", "responsiveness", "and", "rapamycin", "sensitivity", "with", "respect", "to", "specific", "kinase", "activity", ",", "PCNA", "expression", "and", "proliferation", "."]} {"tags": [0, 7, 8, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "L2", "cells", ",", "IL-2", "stimulated", "and", "rapamycin", "inhibited", "the", "following", ":", "cAMP-independent", "CREB", "kinase", "activity", ",", "PCNA", "expression", "and", "proliferation", "."]} {"tags": [0, 7, 8, 8, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "L2", "variant", "cells", ",", "CREB", "kinase", "activity", "was", "constitutively", "high", ";", "IL-2", "stimulated", "and", "rapamycin", "blocked", "PCNA", "expression", "and", "proliferation", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 7, 8, 0], "tokens": ["These", "results", "indicate", "that", "IL-2", "induces", "a", "rapamycin-sensitive", ",", "cAMP-independent", "CREB", "kinase", "activity", "in", "L2", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["However", ",", "phosphorylation", "of", "CREB", "alone", "is", "not", "sufficient", "to", "drive", "PCNA", "expression", "and", "L2", "cell", "proliferation", "in", "the", "absence", "of", "IL-2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 5, 6, 6, 6, 0], "tokens": ["Serotonin", "derivative", ",", "N-", "(", "p-coumaroyl", ")", "serotonin", ",", "inhibits", "the", "production", "of", "TNF-alpha", ",", "IL-1alpha", ",", "IL-1beta", ",", "and", "IL-6", "by", "endotoxin-stimulated", "human", "blood", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "reported", "that", "N-", "(", "p-coumaroyl", ")", "serotonin", "(", "CS", ")", "and", "its", "derivatives", "with", "antioxidative", "activity", "are", "present", "in", "safflower", "seeds", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["As", "reactive", "oxygen", "species", "(", "ROS", ")", "are", "implicated", "in", "the", "signaling", "of", "lipopolysaccharide", "(", "LPS", ")", ",", "we", "examined", "whether", "CS", "has", "a", "suppressive", "effect", "on", "inflammatory", "cytokine", "generation", "from", "human", "monocyte", "s", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0], "tokens": ["CS", "at", "50-200", "microM", "reduced", "tumor", "necrosis", "factor", "(", "TNF", ")", ",", "interleukin-1", "(", "IL-1", ")", ",", "and", "IL-6", "activities", "in", "the", "culture", "supernatants", "from", "LPS-stimulated", "human", "blood", "monocytes", "without", "cytotoxicity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["ELISA", "assay", "revealed", "that", "the", "production", "of", "TNF-alpha", ",", "IL-1alpha", ",", "IL-1beta", ",", "and", "IL-6", "was", "inhibited", "by", "CS", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 5, 0, 0, 0, 0, 0], "tokens": ["Northern", "blot", "analysis", "showed", "that", "LPS-induced", "expression", "of", "these", "cytokine", "mRNA", "in", "monocytes", "was", "suppressed", "by", "CS", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NF-kappaB", "activation", "was", "also", "inhibited", "by", "CS", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 3, 0, 0], "tokens": ["These", "findings", "indicate", "that", "CS", "has", "a", "suppressive", "effect", "on", "proinflammatory", "cytokine", "production", "from", "monocytes", ",", "and", "this", "effect", "is", "based", "in", "part", "on", "the", "suppression", "of", "cytokine", "mRNA", "expression", "through", "inhibition", "of", "NF-kappaB", "activation", "."]} {"tags": [0, 0, 0, 9, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Elevated", "expression", "of", "differentiation", "inhibitory", "factor", "nm23", "mRNA", "in", "monoblastic", "crisis", "of", "a", "patient", "with", "chronic", "myelogenous", "leukemia", "."]} {"tags": [3, 4, 4, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Differentiation", "inhibitory", "factor", "nm23", "gene", "has", "been", "found", "to", "be", "expressed", "in", "high", "quantities", "in", "acute", "myelogenous", "leukemia", "(", "AML", ")", ",", "especially", "in", "acute", "monocytic", "leukemia", "(", "AML-M5", ")", "and", "is", "suggested", "as", "a", "new", "prognostic", "factor", "in", "AML-M5", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "report", "an", "example", "of", "elevated", "expression", "of", "nm23", "mRNA", "in", "a", "patient", "with", "chronic", "myelogenous", "leukemia", "(", "CML", ")", "who", "developed", "monoblastic", "crisis", "."]} {"tags": [0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Relative", "levels", "of", "nm23-H1", "and", "-H2", "mRNA", "extracted", "from", "the", "patient", "'s", "peripheral", "blood", "mononuclear", "cells", "and", "bone", "marrow", "mononuclear", "cells", "were", "measured", "by", "quantitative", "reverse", "transcriptase", "polymerase", "chain", "reaction", "."]} {"tags": [0, 0, 0, 9, 10, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["The", "level", "of", "nm23-H1", "mRNA", "in", "CML", "cells", "at", "the", "chronic", "phase", "was", "as", "high", "as", "that", "in", "bone", "marrow", "cells", "from", "healthy", "volunteers", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "mRNA", "level", "of", "nm23-H2", "was", "slightly", "below", "the", "normal", "level", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["At", "blastic", "crisis", ",", "however", ",", "expression", "of", "both", "nm23-H1", "and", "-H2", "mRNA", "was", "elevated", "to", "about", "three", "to", "nine", "times", "of", "that", "at", "the", "chronic", "phase", "."]} {"tags": [5, 6, 6, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Proliferated", "blastic", "cells", "were", "positive", "for", "non-specific", "esterase", ",", "and", "the", "serum", "lysozyme", "level", "was", "elevated", "and", "diagnosed", "as", "monoblastic", "crisis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "patient", "received", "combined", "chemotherapy", "but", "response", "was", "partial", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0], "tokens": ["These", "findings", "are", "compatible", "with", "our", "previous", "report", "that", "nm23", "gene", "is", "overexpressed", "in", "monocytic", "leukemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 5, 6, 0], "tokens": ["Increased", "transcription", "decreases", "the", "spontaneous", "mutation", "rate", "at", "the", "thymidine", "kinase", "locus", "in", "human", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transcription", "increases", "DNA", "repair", "efficiency", "and", "modulates", "the", "distribution", "of", "certain", "types", "of", "DNA", "damage", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "increased", "transcription", "level", "stimulates", "spontaneous", "mutation", "rate", "in", "yeast", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["We", "explored", "whether", "transcription", "level", "affects", "spontaneous", "mutation", "rate", "in", "human", "cells", "."]} {"tags": [0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0], "tokens": ["We", "first", "developed", "two", "thymidine", "kinase", "(", "tk", ")", "inducible", "human", "cell", "lines", "using", "the", "Gal4-Estrogen", "receptor", "system", "."]} {"tags": [0, 0, 7, 8, 8, 8, 8, 8, 8, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["In", "our", "TK6i-G3", "and", "G9", "tk", "heterozygous", "cell", "lines", ",", "the", "active", "tk", "allele", "is", "linked", "to", "an", "inducible", "promoter", "element", "."]} {"tags": [9, 10, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tk", "mRNA", "is", "induced", "following", "treatment", "with", "estrogen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Spontaneous", "mutation", "rate", "was", "significantly", "decreased", "in", "human", "cell", "lines", "after", "induction", "in", "contrast", "to", "the", "report", "in", "yeast", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "humans", "may", "have", "evolved", "different", "or", "additional", "mechanisms", "to", "deal", "with", "transcription", "related", "spontaneous", "mutagenesis", "."]} {"tags": [0, 0, 0, 0, 0, 0], "tokens": ["Copyright", "1998", "Elsevier", "Science", "B.V", "."]} {"tags": [0, 0, 0, 0], "tokens": ["All", "rights", "reserved", "."]} {"tags": [0, 0, 1, 2, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Mutation", "of", "BCL-6", "gene", "in", "normal", "B", "cells", "by", "the", "process", "of", "somatic", "hypermutation", "of", "Ig", "genes", "."]} {"tags": [1, 2, 2, 2, 2, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Immunoglobulin", "(", "Ig", ")", "genes", "are", "hypermutated", "in", "B", "lymphocytes", "that", "are", "the", "precursors", "to", "memory", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "mutations", "are", "linked", "to", "transcription", "initiation", ",", "but", "non-Ig", "promoters", "are", "permissible", "for", "the", "mutation", "process", ";", "thus", ",", "other", "genes", "expressed", "in", "mutating", "B", "cells", "may", "also", "be", "subject", "to", "somatic", "hypermutation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["Significant", "mutations", "were", "not", "observed", "in", "c-MYC", ",", "S14", ",", "or", "alpha-fetoprotein", "(", "AFP", ")", "genes", ",", "but", "BCL-6", "was", "highly", "mutated", "in", "a", "large", "proportion", "of", "memory", "B", "cells", "of", "normal", "individuals", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["The", "mutation", "pattern", "was", "similar", "to", "that", "of", "Ig", "genes", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["CD28-mediated", "activation", "in", "CD45RA+", "and", "CD45RO+", "T", "cells", ":", "enhanced", "levels", "of", "reactive", "oxygen", "intermediates", "and", "c-Rel", "nuclear", "translocation", "in", "CD45RA+", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0], "tokens": ["We", "have", "analyzed", "the", "effect", "of", "complete", "T", "cell", "activation", "(", "anti-CD3", "plus", "anti-CD28", ")", "on", "the", "activation", "of", "NF-kappaB", "in", "CD45RA+", "(", "naive", ")", "and", "CD45RO+", "(", "memory/effector", ")", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 5, 6, 0], "tokens": ["Long", "exposure", "(", "24", "h", ")", "induced", "stronger", "NF-kappaB", "DNA", "binding", "in", "CD45RA+", "cells", "than", "in", "CD45RO+", "cells", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0], "tokens": ["Analysis", "of", "the", "nuclear", "c-Rel", "protein", "indicated", "that", "after", "anti-CD3+anti-CD28", "stimulation", "the", "level", "of", "c-Rel", "was", "higher", "in", "CD45RA+", "cells", "."]} {"tags": [0, 0, 0, 3, 4, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Analysis", "of", "the", "cytoplasmic", "inhibitor", "IkappaBalpha", "indicated", "that", "anti-CD3+anti-CD28", "stimulation", "induced", "a", "long-lasting", "degradation", "in", "CD45RA+", "cells", "but", "in", "CD45RO+", "cells", "the", "degradation", "process", "was", "more", "rapid", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Because", "the", "CD28", "costimulus", "is", "known", "to", "induce", "the", "production", "of", "reactive", "oxygen", "intermediates", "(", "ROIs", ")", ",", "the", "intracellular", "ROI", "levels", "in", "CD45RA+", "and", "CD45RO+", "cells", "were", "compared", "by", "flow", "cytometry", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["ROIs", "were", "produced", "in", "both", "cell", "types", ",", "but", "more", "strongly", "in", "CD45RA+", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["The", "data", "presented", "in", "this", "study", "further", "emphasize", "the", "differences", "between", "CD45RA+", "and", "CD45RO+", "T", "lymphocytes", "in", "ROI-dependent", "signaling", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Non-Hodgkin", "'s", "lymphoma", "involving", "bilateral", "breasts", "[", "see", "comments", "]"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "describe", "here", "two", "cases", "of", "diffuse", "large", "cell", "type", "non-Hodgkin", "'s", "lymphoma", "affecting", "the", "bilateral", "breasts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "contralateral", "tumor", "in", "one", "case", "appeared", "17", "months", "after", "the", "first", "mastectomy", ",", "whereas", "the", "bilateral", "tumors", "occurred", "concurrently", "in", "the", "other", "patient", "who", "was", "pregnant", "and", "showed", "widespread", "dissemination", "at", "initial", "presentation", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Lymphoma", "cells", "from", "both", "cases", "showed", "the", "mature", "B-cell", "immunophenotype", "and", "had", "rearrangements", "of", "the", "BCL6", "gene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Both", "patients", "developed", "progressive", "disease", "despite", "chemo-radiotherapy", "and", "died", "of", "leukemic", "manifestations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "were", "no", "apparent", "pathological", "features", "of", "lymphomas", "of", "mucosa-associated", "lymphoid", "tissue", "origin"]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Biochemical", "characterization", "of", "MIP-1", "alpha", "nuclear", "protein", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["A", "family", "of", "hematopoietic", "specific", "transcription", "factors", ",", "MIP-1", "alpha", "nuclear", "protein", "(", "MNP", ")", "family", ",", "has", "recently", "been", "identified", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 5, 0, 5, 0, 0, 5, 6, 0], "tokens": ["They", "are", "intimately", "involved", "in", "regulating", "the", "transcription", "of", "the", "huMIP-1", "alpha", "gene", "in", "monocytes", ",", "T-cells", ",", "and", "transformed", "B-cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 0, 5, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["One", "member", "of", "the", "family", "(", "MNP-1", ")", "is", "essential", "for", "promoter", "activity", "in", "monocytes", "and", "B-cells", ",", "while", "another", "(", "MNP-2", ")", "is", "required", "for", "full", "promotor", "activity", "in", "T-cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "third", "member", "of", "the", "family", "(", "MNP-3", ")", "is", "expressed", "in", "PMA", "induced", "HL60", "cells", "and", "probably", "has", "a", "role", "in", "monocyte", "differentiation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0], "tokens": ["In", "this", "communication", "we", "demonstrate", "by", "two", "techniques", "that", "MNP-1", "and", "MNP-2", "are", "distinct", "but", "related", "factors", ",", "and", "we", "present", "further", "evidence", "to", "show", "that", "MNP-1", "acts", "as", "a", "heterodimer", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 5, 6, 6, 6, 0], "tokens": ["Bcl-3", "expression", "and", "nuclear", "translocation", "are", "induced", "by", "granulocyte-macrophage", "colony-stimulating", "factor", "and", "erythropoietin", "in", "proliferating", "human", "erythroid", "precursors", "."]} {"tags": [3, 0, 0, 1, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Bcl-3", "is", "a", "proto-oncogene", "involved", "in", "the", "chromosomal", "translocation", "t", "(", "14", ";", "19", ")", "found", "in", "some", "patients", "with", "chronic", "lymphocytic", "leukemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["It", "shares", "structural", "similarities", "with", "and", "is", "a", "member", "of", "the", "IkappaB", "family", "of", "proteins", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "report", ",", "involvement", "of", "Bcl-3", "in", "hematopoietic", "growth", "factor-stimulated", "erythroid", "proliferation", "and", "differentiation", "was", "examined", "."]} {"tags": [0, 7, 8, 0, 0, 7, 8, 8, 0, 3, 4, 4, 0, 3, 0, 0, 3, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "TF-1", "cells", ",", "an", "erythroleukemia", "cell", "line", ",", "granulocyte-macrophage", "colony-stimulating", "factor", "(", "GM-CSF", ")", "and", "erythropoietin", "(", "Epo", ")", "greatly", "enhanced", "Bcl-3", "expression", "at", "both", "the", "protein", "and", "mRNA", "levels", "in", "association", "with", "stimulation", "of", "proliferation", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Bcl-3", "protein", "was", "also", "highly", "expressed", "in", "early", "burst-forming", "unit-erythroid", "(", "BFU-E", ")", "-derived", "erythroid", "precursors", "(", "day", "7", ")", "and", "decreased", "during", "maturation", "(", "days", "10", "and", "14", ")", ",", "suggesting", "that", "Bcl-3", "is", "involved", "in", "normal", "erythroid", "proliferation", "."]} {"tags": [0, 0, 5, 6, 0, 3, 0, 0, 0], "tokens": ["In", "these", "hematopoietic", "cells", ",", "Bcl-3", "was", "hyperphosphorylated", "."]} {"tags": [3, 0, 3, 0, 0, 0, 0, 0, 3, 0], "tokens": ["GM-CSF", "and", "Epo", "modulated", "the", "subcellular", "localization", "of", "Bcl-3", "."]} {"tags": [0, 0, 0, 7, 8, 0, 3, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Upon", "stimulation", "of", "TF-1", "cells", "with", "GM-CSF", "or", "Epo", ",", "the", "nuclear", "translocation", "of", "Bcl-3", "was", "dramatically", "enhanced", "."]} {"tags": [0, 0, 3, 0, 7, 8, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 0], "tokens": ["Overexpression", "of", "Bcl-3", "in", "TF-1", "cells", "by", "transient", "transfection", "along", "with", "the", "NF-kappaB", "factors", "p50", "or", "p52", "resulted", "in", "significant", "induction", "of", "an", "human", "immunodeficiency", "virus-type", "1", "(", "HIV-1", ")", "kappaB-TATA-luceriferase", "reporter", "plasmid", ",", "demonstrating", "that", "Bcl-3", "has", "a", "positive", "role", "in", "transactivation", "of", "kappaB-containing", "genes", "in", "erythroid", "cells", "."]} {"tags": [0, 0, 3, 0, 9, 10, 0, 0, 0, 0, 0], "tokens": ["Stimulation", "with", "GM-CSF", "enhanced", "c-myb", "mRNA", "expression", "in", "these", "cells", "."]} {"tags": [3, 0, 0, 0, 0, 7, 8, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Bcl-3", "in", "nuclear", "extracts", "of", "TF-1", "cells", "bound", "to", "a", "kappaB", "enhancer", "in", "the", "c-myb", "promoter", "together", "with", "NF-kappaB2/p52", "and", "this", "binding", "activity", "was", "enhanced", "by", "GM-CSF", "stimulation", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 7, 8, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["Furthermore", ",", "cotransfection", "of", "Bcl-3", "with", "p52", "or", "p50", "in", "TF-1", "cells", "resulted", "in", "significant", "activation", "of", "a", "c-myb", "kappaB-TATA-luceriferase", "reporter", "plasmid", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 1, 0], "tokens": ["These", "findings", "suggest", "that", "Bcl-3", "may", "participate", "in", "the", "transcriptional", "regulation", "of", "certain", "kappaB-containing", "genes", "involved", "in", "hematopoiesis", ",", "including", "c-myb", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Copyright", "1998", "by", "The", "American", "Society", "of", "Hematology", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 4, 4, 4, 4, 0, 0], "tokens": ["5-Lipoxygenase", "compartmentalization", "in", "granulocytic", "cells", "is", "modulated", "by", "an", "internal", "bipartite", "nuclear", "localizing", "sequence", "and", "nuclear", "factor", "kappa", "B", "complex", "formation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["A", "region", "of", "basic", "amino", "acids", "spanning", "residues", "639-656", "in", "the", "human", "5-lipoxygenase", "sequence", "resembles", "a", "consensus", "bipartite", "nuclear", "localizing", "sequence", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0], "tokens": ["A", "synthetic", "peptide", "consisting", "of", "the", "Kaposi", "fibroblast", "growth", "factor", "signal", "sequence", "fused", "to", "the", "5-lipoxygenase639-656", "bipartite", "nuclear", "localizing", "sequence", "has", "a", "prominent", "inhibitory", "effect", "on", "5-lipoxygenase", "catalysis", "in", "granulocytic", "HL-60", "cells", "activated", "by", "calcium", "ionophor", "A23187", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Recombinant", "5-lipoxygenase", "was", "not", "affected", "by", "the", "peptide", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0], "tokens": ["The", "peptide", "also", "inhibited", "redistribution", "of", "5-lipoxygenase", "from", "the", "cytosol", "to", "the", "nuclear", "membrane", "of", "HL-60", "cells", "stimulated", "by", "A23187", "."]} {"tags": [3, 4, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 7, 8, 0, 0], "tokens": ["5-Lipoxygenase", "protein", "was", "detected", "in", "nuclear", "factor", "kappaB", "(", "NF-kappaB", ")", "p65", "subunit", "immunoprecipitate", "fractions", "prepared", "from", "HL-60", "cell", "lysates", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "amount", "of", "5-lipoxygenase", "protein", "coimmunoprecipitated", "by", "NF-kappaB", "antiserum", "was", "increased", "following", "A23187", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["In", "cells", "treated", "with", "agents", "that", "block", "5-lipoxygenase", "translocation", "to", "the", "nucleus", ",", "5-lipoxygenase", "protein", "appearing", "in", "the", "NF-kappaB", "immunoprecipitate", "was", "diminished", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Our", "results", "implicate", "an", "internal", "bipartite", "nuclear", "localizing", "sequence", "as", "a", "regulatory", "domain", "that", "modulates", "5-lipoxygenase", "redistribution", "and", "catalysis", "in", "granulocytic", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Additionally", ",", "our", "results", "suggest", "that", "molecular", "determinants", "which", "govern", "5-lipoxygenase", "and", "NF-kappaB", "redistribution", "to", "the", "nucleus", "may", "be", "coordinately", "controlled", "in", "granulocytic", "cells", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Copyright", "1998", "Academic", "Press", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Inhibition", "of", "CD28/CD3-mediated", "costimulation", "of", "naive", "and", "memory", "human", "T", "lymphocytes", "by", "intracellular", "incorporation", "of", "polyclonal", "antibodies", "specific", "for", "the", "activator", "protein-1", "transcriptional", "complex", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "number", "of", "indirect", "methods", "have", "been", "utilized", "in", "demonstrating", "activator", "protein-1", "transcription", "factor", "function", "in", "IL-2", "promoter", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0], "tokens": ["However", ",", "there", "has", "been", "no", "direct", "demonstration", "that", "activator", "protein-1", "is", "involved", "in", "CD28-dependent", "costimulation", "of", "IL-2", "gene", "transcription", "in", "freshly", "isolated", "naive", "and", "memory", "human", "T", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["To", "address", "this", "issue", ",", "the", "method", "of", "scrape", "loading", "was", "applied", "to", "purified", "peripheral", "blood", "T", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Since", "scrape", "loading", "relies", "on", "adherent", "cells", ",", "peripheral", "blood", "human", "T", "(", "PB-T", ")", "cells", "were", "immobilized", "on", "the", "nonspecific", "cell", "attachment", "factor", "poly-L-lysine", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cells", "scraped", "off", "poly-L-lysine", "in", "the", "presence", "of", "Ig", "FITC", "efficiently", "incorporated", "Ig", ",", "with", "relatively", "uniform", "fluorescence", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T", "cells", "retained", "their", "physical", "parameters", "as", "measured", "by", "forward", "and", "side", "light", "scatter", ",", "and", "functional", "activity", "as", "measured", "by", "costimulation", "of", "proliferation", "and", "IL-2", "production", "after", "being", "scraped", "off", "this", "substrate", "."]} {"tags": [5, 6, 6, 0, 0, 3, 0, 0, 0, 0, 0, 5, 0, 5, 0, 5, 0, 0, 5, 0, 0], "tokens": ["CD28/CD3-costimulated", "T", "cells", "produced", "intracellular", "IL-2", "from", "all", "subsets", "measured", "(", "CD4+", ",", "CD4-", ",", "CD45RO+", ",", "and", "CD45RO-", ")", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0], "tokens": ["IL-2", "production", "and", "intracellular", "accumulation", "in", "nonscraped", "PB-T", "cells", "activated", "with", "CD28/CD3", "coligation", "were", "skewed", "favoring", "CD45RO+", "and", "CD4+", "subsets", ",", "as", "was", "IL-2", "production", "in", "scraped", "PB-T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "intracellular", "incorporation", "of", "Abs", "specific", "for", "c-Fos", "and", "c-Jun", "family", "members", "by", "scrape", "loading", "inhibited", "the", "production", "and", "intracellular", "accumulation", "of", "IL-2", "within", "6", "h", "of", "costimulation", "with", "PMA/ionomycin", ",", "or", "costimulation", "by", "CD28", "and", "CD3", "ligation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Scrape", "loading", "thus", "provides", "an", "efficient", "mechanism", "for", "intracellular", "incorporation", "of", "macromolecules", ",", "and", "the", "first", "direct", "evidence", "that", "c-Fos", "and", "c-Jun", "are", "involved", "in", "transcription", "of", "the", "IL-2", "gene", "within", "its", "correct", "chromosomal", "context", ",", "in", "resting", "human", "T", "lymphocyte", "subpopulations", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 1, 2, 0, 3, 4, 0], "tokens": ["Differential", "expression", "of", "Nur77", "family", "members", "in", "human", "T-lymphotropic", "virus", "type", "1-infected", "cells", ":", "transactivation", "of", "the", "TR3/nur77", "gene", "by", "Tax", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["We", "analyzed", "the", "differential", "expression", "and", "regulation", "of", "three", "members", "of", "the", "Nur77", "transcription", "factor", "family", "by", "the", "human", "T-lymphotropic", "virus", "type", "1", "(", "HTLV-1", ")", "Tax", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0, 7, 8, 8, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "demonstrated", "that", "in", "both", "HTLV-1-infected", "cells", "and", "Tax-expressing", "JPX-9", "cells", ",", "TR3/nur77", "is", "highly", "expressed", ",", "whereas", "neither", "NOR-1", "nor", "NOT", "expression", "is", "detectable", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0], "tokens": ["Transient", "transfection", "analysis", "further", "confirmed", "the", "Tax", "transactivation", "of", "the", "TR3/nur77", "promoter", "but", "not", "the", "NOR-1", "promoter", "in", "different", "cell", "types", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0, 1, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "expression", "of", "a", "luciferase", "reporter", "gene", "driven", "by", "the", "NGFI-B", "(", "rat", "homolog", "of", "TR3/Nur77", ")", "response", "element", "(", "NBRE", ")", "provided", "evidence", "that", "Tax", "-mediated", "transactivation", "resulted", "in", "the", "induction", "of", "a", "functional", "protein", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Cotransfection", "assays", "with", "the", "TR3/nur77", "promoter", "sequence", "or", "the", "NBRE", "binding", "motif", "together", "with", "a", "series", "of", "Tax", "mutants", "have", "shown", "that", "Tax", "-induced", "TR3/nur77", "expression", "is", "mediated", "by", "CREB/ATF-related", "transcription", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Negative", "regulation", "of", "the", "heat", "shock", "transcriptional", "response", "by", "HSBP1", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["In", "response", "to", "stress", ",", "heat", "shock", "factor", "1", "(", "HSF1", ")", "acquires", "rapid", "DNA", "binding", "and", "transient", "transcriptional", "activity", "while", "undergoing", "conformational", "transition", "from", "an", "inert", "non-DNA-binding", "monomer", "to", "active", "functional", "trimers", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Attenuation", "of", "the", "inducible", "transcriptional", "response", "occurs", "during", "heat", "shock", "or", "upon", "recovery", "at", "non-stress", "conditions", "and", "involves", "dissociation", "of", "the", "HSF1", "trimer", "and", "loss", "of", "activity", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["We", "have", "used", "the", "hydrophobic", "repeats", "of", "the", "HSF1", "trimerization", "domain", "in", "the", "yeast", "two-hybrid", "protein", "interaction", "assay", "to", "identify", "heat", "shock", "factor", "binding", "protein", "1", "(", "HSBP1", ")", ",", "a", "novel", ",", "conserved", ",", "76-amino-acid", "protein", "that", "contains", "two", "extended", "arrays", "of", "hydrophobic", "repeats", "that", "interact", "with", "the", "HSF1", "heptad", "repeats", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["HSBP1", "is", "nuclear-localized", "and", "interacts", "in", "vivo", "with", "the", "active", "trimeric", "state", "of", "HSF1", "that", "appears", "during", "heat", "shock", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["During", "attenuation", "of", "HSF1", "to", "the", "inert", "monomer", ",", "HSBP1", "associates", "with", "Hsp70", "."]} {"tags": [3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0], "tokens": ["HSBP1", "negatively", "affects", "HSF1", "DNA-binding", "activity", ",", "and", "overexpression", "of", "HSBP1", "in", "mammalian", "cells", "represses", "the", "transactivation", "activity", "of", "HSF1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 4, 4, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["To", "establish", "a", "biological", "role", "for", "HSBP1", ",", "the", "homologous", "Caenorhabditis", "elegans", "protein", "was", "overexpressed", "in", "body", "wall", "muscle", "cells", "and", "was", "shown", "to", "block", "activation", "of", "the", "heat", "shock", "response", "from", "a", "heat", "shock", "promoter-reporter", "construct", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Alteration", "in", "the", "level", "of", "HSBP1", "expression", "in", "C.", "elegans", "has", "severe", "effects", "on", "survival", "of", "the", "animals", "after", "thermal", "and", "chemical", "stress", ",", "consistent", "with", "a", "role", "for", "HSBP1", "as", "a", "negative", "regulator", "of", "the", "heat", "shock", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Long-range", "transcriptional", "regulation", "of", "cytokine", "gene", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Most", "studies", "on", "the", "control", "of", "cytokine", "gene", "expression", "have", "involved", "the", "functional", "analysis", "of", "proximal", "promoters", "."]} {"tags": [0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Recent", "work", "has", "identified", "distal", "elements", "that", "mediate", "long-range", "cytokine", "gene", "regulation", "and", "has", "implicated", "chromatin", "reorganization", "in", "regulation", "of", "cytokine", "gene", "loci", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["These", "studies", "have", "begun", "to", "elucidate", "the", "basis", "for", "cell-specificity", "and", "high-level", "expression", "of", "cytokine", "genes", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["A", "signaling", "complex", "of", "Ca2+-calmodulin-dependent", "protein", "kinase", "IV", "and", "protein", "phosphatase", "2A", "[", "see", "comments", "]"]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0, 3, 0, 0], "tokens": ["Stimulation", "of", "T", "lymphocytes", "results", "in", "a", "rapid", "increase", "in", "intracellular", "calcium", "concentration", "(", "[", "Ca2+", "]", "i", ")", "that", "parallels", "the", "activation", "of", "Ca2+-calmodulin-dependent", "protein", "kinase", "IV", "(", "CaMKIV", ")", ",", "a", "nuclear", "enzyme", "that", "can", "phosphorylate", "and", "activate", "the", "cyclic", "adenosine", "monophosphate", "(", "cAMP", ")", "response", "element-binding", "protein", "(", "CREB", ")", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "inactivation", "of", "CaMKIV", "occurs", "despite", "the", "sustained", "increase", "in", "[", "Ca2+", "]", "i", "that", "is", "required", "for", "T", "cell", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["A", "stable", "and", "stoichiometric", "complex", "of", "CaMKIV", "with", "protein", "serine-threonine", "phosphatase", "2A", "(", "PP2A", ")", "was", "identified", "in", "which", "PP2A", "dephosphorylates", "CaMKIV", "and", "functions", "as", "a", "negative", "regulator", "of", "CaMKIV", "signaling", "."]} {"tags": [0, 7, 8, 8, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0], "tokens": ["In", "Jurkat", "T", "cells", ",", "inhibition", "of", "PP2A", "activity", "by", "small", "t", "antigen", "enhanced", "activation", "of", "CREB", "-mediated", "transcription", "by", "CaMKIV", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0], "tokens": ["These", "findings", "reveal", "an", "intracellular", "signaling", "mechanism", "whereby", "a", "protein", "serine-threonine", "kinase", "(", "CaMKIV", ")", "is", "regulated", "by", "a", "tightly", "associated", "protein", "serine-threonine", "phosphatase", "(", "PP2A", ")", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Hypoxia", "down-regulates", "MCP-1", "expression", ":", "implications", "for", "macrophage", "distribution", "in", "tumors", "."]} {"tags": [3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Monocyte", "chemoattractant", "protein", "1", "(", "MCP-1", ")", "is", "likely", "to", "contribute", "to", "the", "macrophage", "infiltrate", "in", "human", "ovarian", "carcinomas", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "MCP-1", "is", "predominantly", "expressed", "by", "the", "tumor", "parenchyma", ",", "macrophages", "accumulate", "at", "highest", "density", "in", "necrotic", "regions", ",", "which", "are", "associated", "with", "low", "oxygen", "tensions", "."]} {"tags": [3, 4, 4, 4, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tumor", "necrosis", "factor", "alpha", "(", "TNF-alpha", ")", "can", "stimulate", "MCP-1", "production", "and", "is", "also", "present", "within", "ovarian", "carcinomas", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0], "tokens": ["We", "have", "investigated", "the", "effect", "of", "hypoxia", "both", "on", "MCP-1", "expression", "in", "ovarian", "cancer", "cell", "lines", "and", "monocyte", "migration", "."]} {"tags": [0, 0, 9, 10, 10, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Hypoxia", "down-regulated", "TNF-alpha-induced", "MCP-1", "mRNA", "and", "protein", "production", "by", "ovarian", "cancer", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "effect", "was", "mimicked", "by", "cobalt", "chloride", "and", "desferrioxamine", ",", "consistent", "with", "a", "specific", "oxygen-sensing", "mechanism", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Unlike", "antioxidants", ",", "hypoxia", "did", "not", "inhibit", "nuclear", "factor", "KB", "mobilization", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Monocyte", "migration", "in", "response", "to", "MCP-1", "was", "also", "diminished", "under", "hypoxic", "conditions", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["Down-regulation", "of", "MCP-1", "expression", "and", "the", "inhibition", "of", "monocyte", "migration", "are", "independent", "effects", "of", "hypoxia", "that", "may", "contribute", "to", "the", "distribution", "of", "macrophages", "within", "ovarian", "tumors", "."]} {"tags": [0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Activation", "of", "nuclear", "factor", "kappa", "B", "inflammatory", "bowel", "disease", "[", "see", "comments", "]"]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BACKGROUND", ":", "Expression", "of", "pro-inflammatory", "cytokines", "is", "increased", "in", "the", "intestinal", "lamina", "propria", "of", "patients", "with", "inflammatory", "bowel", "disease", "(", "IBD", ")", "."]} {"tags": [3, 4, 4, 4, 0, 3, 4, 4, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Nuclear", "factor", "kappa", "B", "(", "NF", "kappa", "B", ")", "controls", "transcription", "of", "inflammation", "genes", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0], "tokens": ["On", "activation", ",", "NF", "kappa", "B", "is", "rapidly", "released", "from", "its", "cytoplasmic", "inhibitor", "(", "I", "kappa", "B", ")", ",", "transmigrates", "into", "the", "nucleus", ",", "and", "binds", "to", "DNA", "response", "elements", "in", "gene", "promoter", "regions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["AIMS", ":", "To", "investigate", "whether", "increased", "activation", "of", "NF", "kappa", "B", "is", "important", "in", "IBD", "and", "may", "be", "down-regulated", "by", "anti-inflammatory", "treatment", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["METHODS", ":", "Activation", "of", "NF", "kappa", "B", "was", "determined", "by", "western", "blot", "assessment", "and", "electrophoretic", "mobility", "shift", "assay", "in", "nuclear", "extracts", "of", "colonic", "biopsy", "samples", "as", "well", "as", "lamina", "propria", "mononuclear", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "Nuclear", "levels", "of", "NF", "kappa", "B", "p65", "are", "increased", "in", "lamina", "propria", "biopsy", "specimens", "from", "patients", "with", "Crohn", "'s", "disease", "in", "comparison", "with", "patients", "with", "ulcerative", "colitis", "and", "controls", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Increased", "activation", "of", "NF", "kappa", "B", "was", "detected", "in", "lamina", "propria", "mononuclear", "cells", "from", "patients", "with", "active", "IBD", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Corticosteroids", "strongly", "inhibit", "intestinal", "NF", "kappa", "B", "activation", "in", "IBD", "in", "vivo", "and", "in", "vitro", "by", "stabilising", "the", "cytosolic", "inhibitor", "I", "kappa", "B", "alpha", "against", "activation", "induced", "degradation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSIONS", ":", "In", "both", "IBDs", ",", "but", "particularly", "Crohn", "'s", "disease", ",", "increased", "activation", "of", "NF", "kappa", "B", "may", "be", "involved", "in", "the", "regulation", "of", "the", "inflammatory", "response", "."]} {"tags": [0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "NF", "kappa", "B", "activation", "may", "represent", "a", "mechanism", "by", "which", "steroids", "exert", "an", "anti-inflammatory", "effect", "in", "IBD"]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Insufficient", "glycemic", "control", "increases", "nuclear", "factor-kappa", "B", "binding", "activity", "in", "peripheral", "blood", "mononuclear", "cells", "isolated", "from", "patients", "with", "type", "1", "diabetes", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["OBJECTIVE", ":", "The", "redox-sensitive", "transcription", "factor", "nuclear", "factor-kappa", "B", "(", "NF-kappa", "B", ")", "is", "believed", "to", "contribute", "to", "late", "diabetic", "complications", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["It", "is", "unknown", "whether", "NF-kappa", "B", "is", "influenced", "by", "glycemic", "control", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 6, 6, 0, 5, 0, 0], "tokens": ["RESEARCH", "DESIGN", "AND", "METHODS", ":", "To", "determine", "whether", "NF-kappa", "B", "is", "activated", "in", "patients", "with", "insufficient", "glycemic", "control", "(", "HbA1c", ">", "10", "%", ")", ",", "we", "developed", "a", "tissue", "culture-independent", "electrophoretic", "mobility", "shift", "assay", "(", "EMSA", ")", "-based", "semiquantitative", "detection", "system", "that", "allowed", "us", "to", "determine", "NF-kappa", "B", "activation", "in", "ex", "vivo-isolated", "peripheral", "blood", "mononuclear", "cells", "(", "PBMCs", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "included", "43", "patients", "with", "type", "1", "diabetes", "in", "this", "cross-sectional", "study", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["10", "of", "those", "received", "the", "antioxidant", "thioctic", "acid", "(", "600", "mg/day", "p.o.", ")", "for", "2", "weeks", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "Monocytes", "of", "patients", "with", "HbA1c", "levels", ">", "10", "%", "demonstrated", "significantly", "higher", "NF-kappa", "B", "binding", "activity", "in", "an", "EMSA", "and", "a", "stronger", "NF-kappa", "B", "staining", "in", "immunohistochemistry", "than", "monocytes", "of", "patients", "with", "HbA1c", "levels", "of", "6-8", "%", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "increase", "in", "NF-kappa", "B", "activation", "correlated", "with", "an", "increase", "in", "plasmatic", "markers", "of", "lipid", "peroxidation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["Treatment", "with", "the", "antioxidant", "thioctic", "acid", "decreased", "NF-kappa", "B", "binding", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSIONS", ":", "Hyperglycemia", "induces", "activation", "of", "the", "transcription", "factor", "NF-kappa", "B", "in", "ex", "vivo-isolated", "PBMCs", "of", "patients", "with", "type", "1", "diabetes", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["NF-kappa", "B", "activation", "is", "at", "least", "partially", "dependent", "on", "oxidative", "stress", ",", "since", "the", "antioxidant", "thioctic", "acid", "significantly", "lowered", "the", "extent", "of", "NF-kappa", "B", "binding", "activity", "."]} {"tags": [0, 0, 0, 3, 4, 0, 3, 0, 0, 3, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Synergistic", "activation", "of", "MAP", "kinase", "(", "ERK1/2", ")", "by", "erythropoietin", "and", "stem", "cell", "factor", "is", "essential", "for", "expanded", "erythropoiesis", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Stem", "cell", "factor", "(", "SCF", ")", "and", "erythropoietin", "(", "EPO", ")", "work", "synergistically", "to", "support", "erythropoiesis", ",", "but", "the", "mechanism", "for", "this", "synergism", "is", "unknown", "."]} {"tags": [0, 0, 7, 8, 8, 8, 8, 0, 7, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["By", "using", "purified", "human", "erythroid", "colony-forming", "cells", "(", "ECFC", ")", ",", "we", "have", "found", "that", "SCF", "and", "EPO", "synergistically", "activate", "MAP", "kinase", "(", "MAPK", ",", "ERK1/2", ")", ",", "which", "correlates", "with", "the", "cell", "growth", "and", "thus", "may", "be", "responsible", "for", "the", "synergistic", "effects", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Treatment", "of", "the", "cells", "with", "PD98059", "and", "wortmannin", ",", "inhibitors", "of", "MEK", "and", "PI-3", "kinase", ",", "respectively", ",", "inhibited", "the", "synergistic", "activation", "of", "MAPK", "and", "also", "the", "cell", "growth", ",", "further", "supporting", "this", "conclusion", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Wortmannin", "only", "inhibits", "MAPK", "activation", "induced", "by", "EPO", "but", "not", "that", "by", "SCF", ",", "suggesting", "that", "SCF", "and", "EPO", "may", "activate", "MAPK", "through", "different", "pathways", ",", "which", "would", "facilitate", "synergy", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Furthermore", ",", "EPO", ",", "but", "not", "SCF", ",", "led", "to", "activation", "of", "STAT5", ",", "whereas", "SCF", "and", "wortmannin", "had", "no", "effect", "on", "the", "EPO", "-induced", "STAT5", "activation", ",", "suggesting", "that", "STAT5", "is", "not", "involved", "in", "the", "synergistic", "action", "of", "SCF", "and", "EPO", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Together", ",", "the", "data", "suggest", "that", "synergistic", "activation", "of", "MAPK", "by", "SCF", "and", "EPO", "is", "essential", "for", "expanded", "erythropoiesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Copyright", "1998", "by", "The", "American", "Society", "of", "Hematology", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["The", "molecular", "and", "phenotypic", "profile", "of", "primary", "central", "nervous", "system", "lymphoma", "identifies", "distinct", "categories", "of", "the", "disease", "and", "is", "consistent", "with", "histogenetic", "derivation", "from", "germinal", "center-related", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Primary", "central", "nervous", "system", "lymphoma", "(", "PCNSL", ")", "is", "a", "major", "cause", "of", "morbidity", "and", "mortality", "among", "human", "immunodeficiency", "virus", "(", "HIV", ")", "-infected", "individuals", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "precise", "histogenetic", "derivation", "and", "the", "molecular", "pathogenesis", "of", "PCNSL", "is", "poorly", "understood", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "an", "attempt", "to", "clarify", "the", "histogenesis", "and", "pathogenesis", "of", "these", "lymphomas", ",", "49", "PCNSL", "(", "26", "acquired", "immunodeficiency", "syndrome", "[", "AIDS", "]", "-related", "and", "23", "AIDS-unrelated", ")", "were", "analyzed", "for", "multiple", "biologic", "markers", ",", "which", "are", "known", "to", "bear", "histogenetic", "and", "pathogenetic", "significance", "for", "mature", "B-cell", "neoplasms", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["PCNSL", "associated", "frequently", "(", "50.0", "%", ")", "with", "mutations", "of", "BCL-6", "5", "'", "noncoding", "regions", ",", "which", "are", "regarded", "as", "a", "marker", "of", "B-cell", "transition", "through", "the", "germinal", "center", "(", "GC", ")", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Expression", "of", "BCL-6", "protein", ",", "which", "is", "restricted", "to", "GC", "B", "cells", "throughout", "physiologic", "B-cell", "maturation", ",", "was", "detected", "in", "100", "%", "AIDS-unrelated", "PCNSL", "and", "in", "56.2", "%", "AIDS-related", "cases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Notably", ",", "among", "AIDS-related", "PCNSL", ",", "expression", "of", "BCL-6", "was", "mutually", "exclusive", "with", "expression", "of", "Epstein-Barr", "virus", "(", "EBV", ")", "-encoded", "latent", "membrane", "protein", "(", "LMP", ")", "-1", "and", ",", "with", "few", "exceptions", ",", "also", "of", "BCL-2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 5, 6, 6, 0], "tokens": ["All", "but", "one", "PCNSL", "expressed", "hMSH2", ",", "which", "among", "mature", "B", "cells", "selectively", "stains", "GC", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0], "tokens": ["These", "data", "suggest", "that", "PCNSL", "may", "be", "frequently", "related", "to", "GC", "B", "cells", "and", "may", "be", "segregated", "into", "two", "major", "biologic", "categories", "based", "on", "the", "expression", "pattern", "of", "BCL-6", ",", "LMP-1", ",", "and", "BCL-2", "."]} {"tags": [3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BCL-6", "(", "+", ")", "/", "LMP-1", "(", "-", ")", "/", "BCL-2", "(", "-", ")", "PCNSL", "occur", "both", "in", "the", "presence", "and", "in", "the", "absence", "of", "HIV", "infection", "and", "consistently", "display", "a", "large", "noncleaved", "cell", "morphology", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Conversely", ",", "BCL-6", "(", "-", ")", "/", "LMP-1", "(", "+", ")", "/", "BCL-2", "(", "+", ")", "PCNSL", "are", "restricted", "to", "HIV-infected", "hosts", "and", "are", "represented", "by", "lymphomas", "with", "immunoblastic", "features", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "are", "relevant", "for", "the", "pathogenesis", "and", "histogenesis", "of", "PCNSL", "and", "may", "be", "helpful", "to", "segregate", "distinct", "biologic", "and", "prognostic", "categories", "of", "these", "lymphomas", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Copyright", "1998", "by", "The", "American", "Society", "of", "Hematology", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 5, 6, 0], "tokens": ["Antioxidant", "regulation", "of", "phorbol", "ester-induced", "adhesion", "of", "human", "Jurkat", "T-cells", "to", "endothelial", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Regulation", "of", "adhesion", "molecule", "expression", "and", "function", "by", "reactive", "oxygen", "species", "via", "specific", "redox", "sensitive", "mechanisms", "have", "been", "reported", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 5, 6, 0, 0, 0], "tokens": ["The", "effects", "of", "clinically", "safe", "antioxidants", "in", "the", "regulation", "of", "adhesion", "molecule", "expression", "in", "human", "endothelial", "cells", "(", "ECV", ")", ",", "and", "adherence", "of", "human", "Jurkat", "T", "cells", "to", "ECV", "cells", "were", "investigated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "thiol", "antioxidant", ",", "alpha-lipoate", ",", "at", "clinically", "relevant", "doses", "down-regulated", "phorbol", "12-myristate", "13-acetate", "(", "PMA", ")", "-induced", "adhesion", "molecule", "expression", "and", "cell-cell", "adhesion", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "PMA-induced", "ICAM-1", "and", "VCAM-1", "expression", "as", "well", "as", "PMA-induced", "adhesion", "of", "Jurkat", "T-cells", "to", "ECV", "cells", "by", "alpha-lipoate", "was", "dose", "dependent", "(", "50-250", "microM", ")", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["The", "effect", "was", "significant", "for", "ICAM-1", "(", "p", "<", ".01", ")", "and", "VCAM-1", "(", "p", "<", ".01", ")", "expression", "in", "cells", "pretreated", "with", "100", "microM", "alpha-lipoate", "compared", "to", "PMA-activated", "untreated", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "PMA-induced", "adhesion", "molecule", "expression", "and", "cell-cell", "adhesion", "was", "more", "pronounced", "when", "a", "combination", "of", "antioxidants", ",", "alpha-lipoate", "and", "alpha-tocopherol", ",", "were", "used", "compared", "to", "the", "use", "of", "either", "of", "these", "antioxidant", "alone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "regulation", "of", "adhesion", "molecule", "expression", "and", "function", "by", "low", "concentration", "of", "antioxidants", "investigated", "does", "not", "appear", "to", "be", "NF-kappaB", "regulated", "or", "transcription", "dependent", "because", "no", "change", "in", "the", "mRNA", "response", "was", "observed", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0], "tokens": ["Protein", "kinase", "C", "(", "PKC", ")", "has", "been", "suggested", "to", "regulate", "PMA-induced", "adhesion", "molecule", "expression", "by", "post-transcriptional", "stabilization", "of", "adhesion", "molecule", "mRNA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Alpha-lipoate", "pretreatment", "did", "not", "influence", "the", "response", "of", "PKC", "activity", "to", "PMA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Oxidants", "are", "known", "to", "be", "involved", "in", "the", "regulation", "of", "cell", "adhesion", "processes", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Treatment", "of", "ECV", "cells", "with", "PMA", "induced", "generation", "of", "intracellular", "oxidants", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Alpha-lipoate", "(", "100", "or", "250", "microM", ")", "treatment", "decreased", "PMA-induced", "generation", "of", "intracellular", "oxidants", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "inhibitory", "effect", "of", "low", "concentration", "of", "alpha-lipaote", "alone", "or", "in", "combination", "with", "alpha-tocopherol", "on", "agonist-induced", "adhesion", "processes", "observed", "in", "this", "study", "may", "be", "of", "potential", "therapeutic", "value", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 1, 2, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Binding", "of", "human", "immunodeficiency", "virus", "type", "1", "to", "CD4", "and", "CXCR4", "receptors", "differentially", "regulates", "expression", "of", "inflammatory", "genes", "and", "activates", "the", "MEK", "/ERK", "signaling", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "previously", "shown", "that", "binding", "of", "human", "immunodeficiency", "virus", "type", "1", "(", "HIV-1", ")", "virions", "to", "CD4", "receptors", "stimulates", "association", "of", "Lck", "with", "Raf-1", "and", "results", "in", "the", "activation", "of", "Raf-1", "kinase", "in", "a", "Ras-independent", "manner", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["In", "the", "present", "study", ",", "we", "demonstrate", "that", "HIV-1", "envelope", "glycoproteins", "of", "both", "T-cell-tropic", "and", "macrophagetropic", "strains", "rapidly", "activate", "the", "ERK/mitogen-activated", "protein", "(", "MAP", ")", "kinase", "pathway", "and", "the", "binding", "of", "nuclear", "transcription", "factors", "(", "AP-1", ",", "NF-kappaB", ",", "and", "C/EBP", ")", "and", "stimulate", "expression", "of", "cytokine", "and", "chemokine", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["The", "activation", "of", "this", "signaling", "pathway", "requires", "functional", "CD4", "receptors", "and", "is", "independent", "of", "binding", "to", "CXCR4", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0], "tokens": ["Binding", "of", "the", "natural", "ligand", "stromal", "cell-derived", "factor", "1", "(", "SDF-1", ")", "to", "CXCR4", ",", "which", "inhibits", "entry", "of", "T-cell-tropic", "HIV-1", ",", "activates", "also", "the", "ERK/", "MAP", "kinase", "pathway", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["However", ",", "SDF-1", "did", "not", "affect", "the", "CD4-mediated", "expression", "of", "cytokine", "and", "chemokine", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "provide", "firm", "molecular", "evidence", "that", "binding", "of", "HIV-1", "envelope", "glycoproteins", "to", "CD4", "receptor", "initiates", "a", "signaling", "pathway", "(", "s", ")", "independent", "of", "the", "binding", "to", "the", "chemokine", "receptor", "that", "leads", "to", "the", "aberrant", "expression", "of", "inflammatory", "genes", "and", "may", "contribute", "significantly", "to", "HIV-1", "replication", "as", "well", "as", "to", "deregulation", "of", "the", "immune", "system", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 3, 4, 0], "tokens": ["Antigen", "receptor", "signaling", "induces", "MAP", "kinase-mediated", "phosphorylation", "and", "degradation", "of", "the", "BCL-6", "transcription", "factor", "."]} {"tags": [0, 1, 2, 0, 0, 3, 4, 4, 4, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "bcl-6", "proto-oncogene", "encodes", "a", "POZ/zinc", "finger", "transcriptional", "repressor", "expressed", "in", "germinal", "center", "(", "GC", ")", "B", "and", "T", "cells", "and", "required", "for", "GC", "formation", "and", "antibody", "affinity", "maturation", "."]} {"tags": [0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Deregulation", "of", "bcl-6", "expression", "by", "chromosomal", "rearrangements", "and", "point", "mutations", "of", "the", "bcl-6", "promoter", "region", "are", "implicated", "in", "the", "pathogenesis", "of", "B-cell", "lymphoma", "."]} {"tags": [0, 0, 0, 1, 0, 0, 0, 0, 0], "tokens": ["The", "signals", "regulating", "bcl-6", "expression", "are", "not", "known", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0], "tokens": ["Here", "we", "show", "that", "antigen", "receptor", "activation", "leads", "to", "BCL-6", "phosphorylation", "by", "mitogen-activated", "protein", "kinase", "(", "MAPK", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0], "tokens": ["Phosphorylation", ",", "in", "turn", ",", "targets", "BCL-6", "for", "rapid", "degradation", "by", "the", "ubiquitin", "/", "proteasome", "pathway", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["These", "findings", "indicate", "that", "BCL-6", "expression", "is", "directly", "controlled", "by", "the", "antigen", "receptor", "via", "MAPK", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0], "tokens": ["This", "signaling", "pathway", "may", "be", "crucial", "for", "the", "control", "of", "B-cell", "differentiation", "and", "antibody", "response", "and", "has", "implications", "for", "the", "regulation", "of", "other", "POZ/zinc", "finger", "transcription", "factors", "in", "other", "tissues", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptors", "are", "differentially", "expressed", "in", "the", "cells", "and", "tissues", "of", "the", "immune", "system", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["Cytosolic", "glucocorticoid", "receptor", "(", "GR", ")", "binding", "studies", "on", "immune", "tissues", "demonstrate", "that", "the", "thymus", "exhibits", "three", "to", "four", "times", "higher", "levels", "of", "GR", "protein", "than", "the", "spleen", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["High", "levels", "of", "GR", "are", "consistent", "with", "the", "exquisite", "sensitivity", "of", "the", "thymus", "to", "glucocorticoid", "exposure", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 6, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Nevertheless", ",", "whole", "cell", "binding", "studies", "reveal", "similar", "levels", "of", "GR", "in", "immature", "thymic", "T", "lymphocytes", "and", "more", "mature", ",", "splenic", "T", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 3, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "whole", "cell", "binding", "techniques", "indicate", "that", "neutrophils", "(", "which", "represent", "roughly", "30", "%", "of", "splenic", "leukocytes", ")", "exhibit", "higher", "GR", "than", "both", "T", "and", "B", "lymphocytes", ",", "further", "contradicting", "results", "from", "cytosolic", "binding", "studies", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["To", "address", "these", "inconsistencies", ",", "GR", "protein", "was", "assessed", "in", "immune", "cells", "and", "tissues", "using", "cytosolic", "radioligand", "binding", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Western", "blot", "analysis", ",", "and", "immunocytochemistry", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Consistent", "with", "previous", "cytosolic", "receptor", "binding", "studies", "on", "immune", "tissue", "homogenates", ",", "thymic", "T", "cells", "were", "found", "to", "have", "higher", "levels", "of", "GR", "than", "T", "cells", "isolated", "from", "the", "spleen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 0, 5, 0], "tokens": ["In", "addition", ",", "neutrophils", "were", "found", "to", "have", "fewer", "GR", "than", "lymphocytes", "and", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "indicate", "a", "meaningful", "relationship", "between", "receptor", "expression", "and", "known", "sensitivity", "to", "glucocorticoids", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 2, 0], "tokens": ["Functional", "replacement", "of", "the", "mouse", "E2A", "gene", "with", "a", "human", "HEB", "cDNA", "."]} {"tags": [0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "mammalian", "E2A", ",", "HEB", ",", "and", "E2-2", "genes", "encode", "a", "unique", "class", "of", "basic", "helix-loop-helix", "(", "bHLH", ")", "transcription", "factors", "that", "are", "evolutionarily", "conserved", "and", "essential", "for", "embryonic", "and", "postnatal", "development", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["While", "the", "structural", "and", "functional", "similarities", "among", "the", "gene", "products", "are", "well", "demonstrated", ",", "it", "is", "not", "clear", "why", "deletion", "of", "E2A", ",", "but", "not", "HEB", "or", "E2-2", ",", "leads", "to", "a", "complete", "arrest", "in", "B-lymphocyte", "development", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 1, 2, 2, 0], "tokens": ["To", "understand", "the", "molecular", "basis", "of", "the", "functional", "specificity", "between", "E2A", "and", "HEB", "/", "E2-2", "in", "mammalian", "development", ",", "we", "generated", "and", "tested", "a", "panel", "of", "E2A", "knockin", "mutations", "including", "subtle", "mutations", "in", "the", "E12", "and", "E47", "exons", "and", "substitution", "of", "both", "E12", "and", "E47", "exons", "with", "a", "human", "HEB", "cDNA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["We", "find", "that", "the", "alternatively", "spliced", "E12", "and", "E47", "bHLH", "proteins", "of", "the", "E2A", "gene", "play", "similar", "and", "additive", "roles", "in", "supporting", "B", "lymphopoiesis", "."]} {"tags": [0, 0, 0, 0, 0, 1, 0, 0, 0, 1, 2, 2, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Further", ",", "we", "find", "that", "HEB", "driven", "by", "the", "endogenous", "E2A", "promoter", "can", "functionally", "replace", "E2A", "in", "supporting", "B-cell", "commitment", "and", "differentiation", "toward", "completion", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0], "tokens": ["Finally", ",", "the", "postnatal", "lethality", "associated", "with", "E2A", "disruption", "is", "fully", "rescued", "by", "the", "addition", "of", "HEB", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "study", "suggests", "that", "the", "functional", "divergence", "among", "E12", ",", "E47", ",", "and", "HEB", "in", "different", "cell", "types", "is", "partially", "defined", "by", "the", "context", "of", "gene", "expression", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 1, 2, 2, 0], "tokens": ["Biased", "dependency", "of", "CD80", "versus", "CD86", "in", "the", "induction", "of", "transcription", "factors", "regulating", "the", "human", "IL-2", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", "to", "the", "signals", "obtained", "by", "ligation", "of", "the", "TCR", ",", "T", "cells", "need", "additional", ",", "co-stimulatory", "signals", "to", "be", "activated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 3, 0, 3, 0, 5, 6, 0, 5, 0, 0], "tokens": ["One", "such", "co-stimulatory", "signal", "is", "delivered", "when", "CD28", "on", "T", "cells", "binds", "to", "CD80", "or", "CD86", "on", "antigen-presenting", "cells", "(", "APC", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 5, 6, 6, 6, 0, 0, 0, 0], "tokens": ["In", "the", "present", "study", ",", "we", "analyzed", "the", "ability", "of", "CD80", "and", "CD86", "to", "co-stimulate", "human", "T", "cells", "activated", "by", "superantigen", "."]} {"tags": [0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Using", "the", "Raji", "B", "cell", "lymphoma", ",", "which", "express", "similar", "levels", "of", "CD80", "and", "CD86", ",", "it", "was", "found", "that", "T", "cell", "proliferation", "was", "mainly", "co-stimulated", "by", "CD80", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 3, 4, 4, 4, 0, 0, 3, 0, 3, 0, 3, 0, 3, 0], "tokens": ["To", "further", "characterize", "the", "consequences", "of", "this", "biased", "co-stimulatory", "dependency", ",", "we", "employed", "a", "well-defined", "system", "of", "transfected", "CHO", "cells", "expressing", "human", "MHC", "class", "II", "together", "with", "CD80", ",", "CD86", "or", "CD80", "and", "CD86", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Proliferation", "of", "freshly", "prepared", "CD4+", "T", "cells", "required", "the", "presence", "of", "either", "CD80", "or", "CD86", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0], "tokens": ["However", ",", "IL-2", "production", "reached", "only", "suboptimal", "levels", "in", "the", "presence", "of", "CD86", "but", "optimal", "levels", "with", "CD80", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 0, 3, 0, 5, 6, 0, 0, 7, 8, 8, 0, 0, 1, 2, 2, 2, 0], "tokens": ["To", "analyze", "IL-2", "transcriptional", "activity", "in", "CD80", "and", "CD86", "co-stimulated", "T", "cells", "we", "used", "Jurkat", "T", "cells", "transfected", "with", "luciferase", "reporter", "gene", "constructs", "."]} {"tags": [3, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 3, 0], "tokens": ["CD80", "induced", "higher", "levels", "of", "IL-2", "promoter", "-enhancer", "activity", "compared", "to", "CD86", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 1, 2, 2, 0, 3, 4, 0, 1, 2, 2, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0], "tokens": ["Furthermore", ",", "the", "activity", "of", "transcription", "factors", "regulating", "the", "IL-2", "promoter-enhancer", "region", "including", "activation", "protein-1", ",", "CD28", "response", "element", "and", "nuclear", "factor", "kappaB", "were", "4-8", "times", "higher", "after", "CD80", "compared", "to", "CD86", "ligation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "results", "suggest", "that", "the", "eventual", "appearance", "of", "CD80", "on", "recently", "activated", "CD86+", "APC", "is", "important", "for", "the", "superinduction", "of", "IL-2", "production", "and", "to", "support", "vigorous", "T", "cell", "proliferation", "."]} {"tags": [0, 0, 0, 1, 2, 0, 1, 2, 2, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Duplication", "of", "the", "DR3", "gene", "on", "human", "chromosome", "1p36", "and", "its", "deletion", "in", "human", "neuroblastoma", "."]} {"tags": [0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 5, 0], "tokens": ["The", "human", "DR3", "gene", ",", "whose", "product", "is", "also", "known", "as", "Wsl-1/APO-3/TRAMP/LARD", ",", "encodes", "a", "tumor", "necrosis", "factor-related", "receptor", "that", "is", "expressed", "primarily", "on", "the", "surface", "of", "thymocytes", "and", "lymphocytes", "."]} {"tags": [3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["DR3", "is", "capable", "of", "inducing", "both", "NF-kappa", "B", "activation", "and", "apoptosis", "when", "overexpressed", "in", "mammalian", "cells", ",", "although", "its", "ligand", "has", "not", "yet", "been", "identified", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0, 0, 0, 0], "tokens": ["We", "report", "here", "that", "the", "DR3", "gene", "locus", "is", "tandemly", "duplicated", "on", "human", "chromosome", "band", "1p36.2-p36.3", "and", "that", "these", "genes", "are", "hemizygously", "deleted", "and/or", "translocated", "to", "another", "chromosome", "in", "neuroblastoma", "(", "NB", ")", "cell", "lines", "with", "amplified", "MYCN", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Duplication", "of", "at", "least", "a", "portion", "of", "the", "DR3", "gene", ",", "including", "the", "extracellular", "and", "transmembrane", "regions", "but", "not", "the", "cytoplasmic", "domain", ",", "was", "demonstrated", "by", "both", "fluorescence", "in", "situ", "hybridization", "and", "genomic", "Southern", "blotting", "."]} {"tags": [0, 0, 7, 8, 8, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 1, 0], "tokens": ["In", "most", "NB", "cell", "lines", ",", "both", "the", "DR3", "and", "the", "DR3L", "sequences", "are", "simultaneously", "deleted", "and/or", "translocated", "to", "another", "chromosome", "."]} {"tags": [0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8], "tokens": ["Finally", ",", "DR3/", "Wsl-1", "protein", "expression", "is", "quite", "variable", "among", "these", "NB", "cell", "lines", ",", "with", "very", "low", "or", "undetectable", "levels", "in", "7", "of", "17", "NB", "cell", "lines"]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Differential", "protection", "of", "normal", "and", "malignant", "human", "myeloid", "progenitors", "(", "CFU-GM", ")", "from", "Ara-C", "toxicity", "using", "cycloheximide", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Cycloheximide", ",", "a", "reversible", "protein", "synthesis", "inhibitor", ",", "is", "thought", "to", "block", "DNA", "replication", "in", "normal", "cells", "by", "preventing", "synthesis", "of", "a", "labile", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "animal", "systems", ",", "cycloheximide", "protects", "normal", "cells", "from", "cytotoxic", "S-phase", "specific", "agents", ",", "such", "as", "cytosine", "arabinoside", "(", "Ara-C", ")", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Malignant", "cells", "appear", "not", "to", "be", "susceptible", "to", "cycloheximide-induced", "cycle", "arrest", "and", ",", "subsequently", ",", "may", "not", "be", "protected", "from", "Ara-C", "cytotoxicity", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 0], "tokens": ["The", "effect", "of", "cycloheximide", "on", "granulocyte/macrophage", "progenitors", "(", "CFU-GM", ")", "after", "in", "vitro", "Ara-C", "exposure", "was", "examined", "using", "normal", "human", "bone", "marrow", ",", "malignant", "progenitors", "from", "patients", "with", "chronic", "myelogenous", "leukemia", "(", "CML", ")", ",", "and", "clonogenic", "cells", "from", "the", "human", "acute", "nonlymphocytic", "leukemia", "cell", "lines", "HL-60", "and", "KG-1", "."]} {"tags": [7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mononuclear", "or", "clonogenic", "cells", "were", "incubated", "for", "one", "hour", "with", "cycloheximide", ",", "followed", "by", "the", "addition", ",", "for", "three", "or", "17", "hours", ",", "of", "Ara-C", "before", "being", "plated", "in", "a", "methylcellulose", "culture", "system", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CFU-GM", "survival", "was", "significantly", "increase", "if", "normal", "cells", "were", "treated", "with", "cycloheximide", "before", "Ara-C", "exposure", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 7, 8, 8, 8, 8, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["Similar", "cycloheximide", "pretreatment", "of", "CML", "progenitors", "and", "clonogenic", "HL-60", "and", "KG-1", "cells", "failed", "to", "protect", "CFU-GM", "from", "Ara-C-induced", "cytotoxicity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Primary", "cortisol", "resistance", "accompanied", "by", "a", "reduction", "in", "glucocorticoid", "receptors", "in", "two", "members", "of", "the", "same", "family", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "report", "describes", "studies", "of", "a", "man", "suspected", "of", "having", "primary", "cortisol", "resistance", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "conclusion", "is", "based", "on", "his", "high", "plasma", "cortisol", "levels", "and", "high", "24-h", "urinary", "17-hydroxycorticosteroid", "and", "cortisol", "excretion", ",", "plus", "the", "fact", "that", "he", "had", "no", "manifestations", "of", "Cushing", "'s", "syndrome", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Among", "family", "members", "tested", ",", "his", "mother", "also", "had", "hypercortisolemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Both", "mother", "and", "son", "had", "high", "levels", "of", "unbound", "plasma", "cortisol", ",", "but", "their", "plasma", "ACTH", "concentrations", "were", "within", "the", "normal", "range", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Both", "were", "partially", "resistant", "to", "dexamethasone", "adrenal", "suppression", ",", "and", "both", "had", "mild", "hypertension", "without", "hypokalemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0], "tokens": ["To", "study", "this", "apparent", "end-organ", "resistance", "to", "cortisol", ",", "we", "examined", "the", "glucocorticoid", "receptors", "in", "peripheral", "mononuclear", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "whole", "cell", "assays", ",", "glucocorticoid", "receptors", "in", "both", "patients", "were", "found", "to", "have", "reduced", "total", "binding", "capacity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["We", "conclude", "that", "these", "two", "patients", ",", "members", "of", "the", "same", "family", ",", "have", "primary", "cortisol", "resistance", "accompanied", "by", "a", "reduced", "number", "of", "glucocorticoid", "receptors", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["[", "Glucocorticoid", "receptors", "and", "response", "to", "polychemotherapy", "in", "acute", "lymphatic", "leukemia", "]"]} {"tags": [3, 4, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptor", "(", "GR", ")", "levels", "were", "quantified", "in", "leukemic", "blasts", "from", "peripheral", "blood", "of", "86", "patients", "with", "acute", "lymphoblastic", "leukemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "subsequent", "achievement", "of", "complete", "remission", "after", "combination", "chemotherapy", "was", "correlated", "with", "high", "receptor", "levels", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Forty-seven", "of", "50", "patients", "with", "leukemic", "cells", "containing", "more", "than", "6", ",", "000", "receptor", "sites", "and", "22", "of", "36", "patients", "with", "cells", "containing", "less", "than", "6", ",", "000", "receptor", "sites", "achieved", "remission", "."]} {"tags": [0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "study", "of", "glucocorticoid", "receptors", "in", "leukemic", "cells", "may", "predict", "response", "to", "combination", "chemotherapy", "in", "patients", "with", "acute", "lymphoblastic", "leukemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["Specific", "uptake", "of", "1", ",", "25-dihydroxycholecalciferol", "by", "human", "chronic", "myeloid", "leukemia", "cells", "."]} {"tags": [0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "examined", "mononuclear", "cell", "preparations", "from", "patients", "with", "chronic", "myeloid", "leukemia", "[", "CML", "]", "for", "binding", "of", "and", "response", "to", "1", ",", "25-dihydroxycholecalciferol", "[", "1", ",", "25-", "(", "OH", ")", "2D3", "]", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Whole", "cells", "specifically", "took", "up", "[", "3H", "]", "-1", ",", "25-", "(", "OH", ")", "2D3", "with", "high", "affinity", "(", "Kd", "3.6", "X", "10", "(", "-11", ")", "M", ")", "and", "low", "capacity", "."]} {"tags": [0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Subcellular", "fractionation", "of", "labeled", "cells", "showed", "that", "binding", "was", "restricted", "to", "cytosols", "and", "nuclei", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Sucrose", "gradient", "centrifugation", "of", "cells", "preincubated", "with", "[", "3H", "]", "-1", ",", "25-", "(", "OH", ")", "2D3", "revealed", "a", "single", "3.6S", "peak", "which", "was", "totally", "displaced", "with", "100-fold", "excess", "nonradioactive", "hormone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "we", "were", "unable", "to", "demonstrate", "specific", "binding", "of", "1", ",", "25-", "(", "OH", ")", "2D3", "by", "postlabeling", "standard", "cytosol", "preparations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0], "tokens": ["In", "addition", ",", "cytosols", "prepared", "from", "a", "mixture", "of", "CML", "cells", "and", "1", ",", "25-", "(", "OH", ")", "2D3", "receptor-positive", "T47D", "(", "human", "breast", "cancer", ")", "cells", "had", "less", "than", "10", "%", "of", "the", "binding", "measured", "in", "T47D", "cytosol", "alone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0], "tokens": ["However", ",", "the", "levels", "of", "binding", "in", "T47D", "cytosols", "were", "not", "reduced", "if", "the", "receptors", "were", "occupied", "with", "[", "3H", "]", "-1", ",", "25-", "(", "OH", ")", "2D3", "prior", "to", "the", "addition", "of", "the", "CML", "cytosols", "."]} {"tags": [0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "CML", "cells", "appear", "to", "contain", "both", "the", "receptor", "for", "1", ",", "25-", "(", "OH", ")", "2D3", "and", "an", "unknown", "substance", "which", "prevents", "its", "detection", "following", "the", "preparation", "of", "cytosol", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cells", "from", "patients", "with", "CML", "in", "the", "chronic", "phase", "specifically", "bound", "more", "1", ",", "25-", "(", "OH", ")", "2D3", "[", "18.0", "+/-", "3.2", "(", "S.E.", ")", "fmol/10", "(", "7", ")", "cells", "]", "than", "did", "those", "in", "acute", "myeloid", "transformation", "[", "7.2", "+/-", "1.5", "]", "or", "than", "did", "cells", "from", "patients", "with", "acute", "myeloid", "leukemia", "[", "2.6", "+/-", "0.8", "]", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Only", "cells", "from", "the", "first", "group", "of", "patients", "responded", "to", "the", "addition", "of", "1", ",", "25-", "(", "OH", ")", "2D3", "by", "differentiating", "along", "the", "monocyte-macrophage", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "conclude", "that", "the", "differentiation-induction", "effect", "of", "1", ",", "25-", "(", "OH", ")", "2D3", "is", "likely", "to", "depend", "on", "adequate", "levels", "of", "receptor", "and", "that", "intact", "cells", "rather", "than", "cytosol", "preparations", "should", "be", "studied", "before", "cells", "of", "a", "particular", "tissue", "are", "designated", "as", "receptor", "negative", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Effect", "of", "cell", "cycle", "position", "on", "dexamethasone", "binding", "by", "mouse", "and", "human", "lymphoid", "cell", "lines", ":", "correlation", "between", "an", "increase", "in", "dexamethasone", "binding", "during", "S", "phase", "and", "dexamethasone", "sensitivity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0], "tokens": ["We", "determined", "the", "effect", "of", "cell", "cycle", "position", "on", "the", "amount", "of", "dexamethasone", "that", "was", "specifically", "bound", "by", "mouse", "and", "human", "lymphoid", "cell", "lines", "."]} {"tags": [7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cell", "lines", "that", "were", "either", "sensitive", "or", "resistant", "to", "growth", "inhibition", "by", "dexamethasone", "were", "compared", "."]} {"tags": [5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Exponentially", "growing", "cells", "were", "separated", "by", "centrifugal", "elutriation", "into", "fractions", "that", "corresponded", "to", "different", "positions", "in", "the", "cell", "cycle", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "cell", "cycle", "phase", "distribution", "of", "each", "fraction", "was", "estimated", "by", "flow", "cytometry", "and", "autoradiography", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "amount", "of", "dexamethasone", "bound", "per", "cell", "in", "each", "fraction", "was", "measured", "by", "a", "whole", "cell", "binding", "assay", "."]} {"tags": [0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "three", "dexamethasone-sensitive", "cell", "lines", "(", "two", "mouse", "and", "one", "human", ")", ",", "we", "found", "that", "the", "amount", "of", "dexamethasone", "bound", "per", "cell", "increased", "2-4-fold", "between", "G1", "phase", "and", "S", "phase", ",", "and", "then", "decreased", "during", "G2/M", "phase", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["Results", "were", "the", "same", "when", "the", "amount", "of", "dexamethasone", "bound", "per", "milligram", "of", "cell", "protein", "was", "measured", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Binding", "affinity", "was", "the", "same", "during", "G1", "phase", "and", "S", "phase", ",", "but", "the", "proportion", "of", "bound", "dexamethasone", "that", "translocated", "to", "the", "nucleus", "was", "greater", "during", "S", "phase", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "we", "found", "that", "the", "amount", "of", "dexamethasone", "bound", "per", "cell", "by", "three", "dexamethasone-resistant", "cell", "lines", "(", "two", "mouse", "and", "one", "human", ")", "did", "not", "increase", "during", "S", "phase", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "results", "indicate", "that", "cell", "cycle", "changes", "in", "dexamethasone", "binding", "are", "not", "simply", "related", "to", "changes", "in", "cell", "protein", "or", "cell", "volume", "during", "the", "cell", "cycle", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["An", "increase", "in", "dexamethasone", "binding", "during", "S", "phase", "may", "be", "required", "for", "dexamethasone", "to", "inhibit", "cell", "growth", ",", "and", "a", "failure", "of", "dexamethasone", "binding", "to", "increase", "during", "S", "phase", "might", "represent", "a", "new", "mechanism", "of", "dexamethasone", "resistance", "in", "lymphoid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0], "tokens": ["Presence", "and", "steroid", "inducibility", "of", "glutamine", "synthetase", "in", "human", "leukemic", "cells", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Glutamine", "synthetase", "(", "EC", "6.3.1.2", ";", "GS", ")", "is", "present", "in", "lymphoblasts", "from", "patients", "with", "acute", "lymphoblastic", "leukemia", "(", "ALL", ")", "as", "well", "as", "in", "normal", "peripheral", "blood", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["In", "16", "out", "of", "20", "ALL", "patients", "studied", "exposure", "of", "the", "cells", "to", "physiological", "concentrations", "of", "dexamethasone", "in", "vitro", "increased", "enzyme", "activity", "above", "the", "control", "levels", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0], "tokens": ["The", "increase", "was", "specific", "for", "glucocorticoid", "receptor", "ligands", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 0], "tokens": ["A", "direct", "correlation", "was", "found", "between", "the", "magnitude", "of", "glucocorticoid-mediated", "increase", "of", "GS", "activity", "and", "the", "cellular", "levels", "of", "specific", "glucocorticoid", "receptors", "assayed", "in", "the", "same", "cell", "specimen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "the", "basal", "levels", "of", "the", "enzyme", "measured", "in", "cells", "prior", "to", "exposure", "to", "dexamethasone", "correlated", "negatively", "with", "receptor", "density", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["It", "is", "suggested", "that", "the", "presence", "of", "steroid-inducible", "GS", "in", "ALL", "cells", "may", "prove", "to", "be", "a", "marker", "for", "functional", "receptor", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Binding", "of", "progestins", "to", "the", "glucocorticoid", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Correlation", "to", "their", "glucocorticoid-like", "effects", "on", "in", "vitro", "functions", "of", "human", "mononuclear", "leukocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "number", "of", "physiological", "and", "synthetic", "progestins", "were", "tested", "for", "their", "ability", "to", "compete", "with", "[", "3H", "]", "dexamethasone", "for", "the", "binding", "to", "the", "glucocorticoid", "receptor", "of", "human", "mononuclear", "leukocytes", "and", "their", "ability", "to", "elicit", "glucocorticoid-like", "effects", "on", "the", "same", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["As", "compared", "to", "the", "reference", "compound", "dexamethasone", "(", "relative", "receptor", "binding", "affinity", "defined", "as", "100", "%", ")", ",", "two", "potent", "synthetic", "progestins", "with", "a", "pregnane-type", "structure", ",", "megestrol", "acetate", "and", "medroxyprogesterone", "acetate", ",", "were", "found", "to", "display", "a", "considerable", "binding", "affinity", "towards", "the", "receptor", "(", "46", "and", "42", "%", ",", "respectively", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "relative", "binding", "affinity", "of", "the", "naturally", "occurring", "ligand", ",", "cortisol", ",", "to", "the", "receptor", "was", "clearly", "lower", "(", "25", "%", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "effective", "binding", "of", "medroxyprogesterone", "acetate", "to", "the", "glucocorticoid", "receptor", "was", "confirmed", "by", "direct", "binding", "studies", "utilizing", "a", "tritiated", "derivative", "of", "this", "steroid", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "evidence", "for", "the", "existence", "of", "a", "specific", "progesterone", "receptor", "in", "human", "mononuclear", "leukocytes", "was", "obtained", "as", "judged", "by", "the", "results", "of", "competition", "experiments", "where", "a", "progesterone", "receptor-specific", "ligand", "[", "3H", "]", "Org", "2058", "was", "used", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["Medroxyprogesterone", "acetate", "and", "megestrol", "acetate", "also", "induced", "glucocorticoid-like", "effects", "on", "the", "lymphocyte", "functions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 4, 0, 3, 0, 0, 0, 0, 0, 5, 6, 6, 0, 7, 8, 8, 0], "tokens": ["These", "included", "inhibition", "of", "the", "proliferative", "responses", "to", "the", "T-cell", "mitogens", "concanavalin", "A", "and", "phytohaemagglutinin", "and", "an", "enhanced", "accumulation", "of", "immunoglobulin", "secreting", "cells", "in", "pokeweed", "mitogen-stimulated", "cultures", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "progestin", "effect", "appears", "to", "be", "mediated", "through", "a", "radiosensitive", "(", "suppressor", ")", "subpopulation", "of", "T", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["In", "contrast", ",", "the", "synthetic", "progestins", "related", "structurally", "to", "19-nortestosterone", ",", "norethisterone", "and", "d-norgestrel", ",", "were", "virtually", "devoid", "of", "binding", "affinity", "towards", "the", "glucocorticoid", "receptor", "nor", "did", "they", "measurably", "influence", "the", "in", "vitro", "lymphocyte", "functions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "studies", "demonstrate", "that", "certain", "progestins", "in", "common", "clinical", "use", "probably", "possess", "inherent", "glucocorticoid", "activity", "and", "suggest", "that", "side", "effects", "attributable", "to", "this", "character", "(", "e.g.", "suppression", "of", "the", "pituitary-adrenal", "axis", ")", "might", "be", "expected", "when", "these", "compounds", "are", "used", "in", "pharmacological", "doses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0], "tokens": ["Metabolic", "and", "ultrastructural", "aspects", "of", "the", "in", "vitro", "lysis", "of", "chronic", "lymphocytic", "leukemia", "cells", "by", "glucocorticoids", "."]} {"tags": [5, 6, 6, 6, 6, 6, 6, 6, 0, 5, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 5, 0, 0, 0, 0, 3, 0], "tokens": ["Human", "chronic", "lymphocytic", "leukemia", "(", "CLL", ")", "cells", "like", "prothymocytes", "and", "immunoactivated", "T-lymphocytes", "are", "readily", "lysed", "in", "vitro", "by", "pharmacological", "concentrations", "of", "glucocorticoids", "such", "as", "cortisol", ",", "whereas", "peripheral", "blood", "lymphocytes", "and", "thymocytes", "are", "unaffected", "by", "the", "hormone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["In", "this", "study", ",", "metabolic", "and", "ultrastructural", "aspects", "of", "the", "cortisol-induced", "killing", "process", "of", "CLL", "cells", "are", "recorded", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "vitro", "lysis", "was", "found", "to", "be", "temperature", "dependent", "and", "was", "detected", "only", "after", "6", "to", "8", "hr", "incubation", "with", "cortisol", "by", "means", "of", "the", "trypan", "blue", "exclusion", "test", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "30", "min", "of", "incubation", "with", "cortisol", "at", "either", "37", "degrees", "or", "4", "degrees", "followed", "by", "the", "removal", "of", "the", "hormone", "was", "still", "sufficient", "to", "induce", "the", "lytic", "process", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Ultrastructural", "studies", "demonstrated", "sequential", "changes", "in", "the", "cytoplasm", ",", "including", "swelling", "of", "mitochondria", "and", "cytoplasmic", "decompartmentalization", ",", "followed", "by", "loss", "of", "surface", "microvilli", "with", "the", "appearance", "of", "``", "holes", "''", "in", "the", "cell", "membrane", ",", "and", "subsequent", "condensation", "of", "nuclear", "chromatin", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "large", "holes", "in", "the", "membrane", "appearing", "after", "6", "hr", "of", "incubation", "with", "the", "hormone", "may", "be", "the", "cause", "for", "the", "penetration", "of", "the", "viable", "stain", "into", "the", "dead", "cells", ",", "as", "seen", "by", "light", "microscopy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Addition", "of", "metabolic", "inhibitors", "including", "actinomycin", "D", ",", "puromycin", ",", "and", "cycloheximide", "following", "administration", "of", "cortisol", "resulted", "in", "inhibition", "of", "the", "cell", "lysis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["An", "excess", "of", "an", "antagonist", "such", "as", "cortexolone", "was", "found", "to", "inhibit", "the", "cortisol-induced", "cytolysis", "of", "the", "CLL", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["It", "is", "suggested", "that", "the", "glucocorticoid-induced", "lysis", "of", "human", "CLL", "cells", "is", "similar", "to", "the", "phenomenon", "observed", "in", "rat", "or", "murine", "lymphocytes", "and", "is", "mediated", "by", "interaction", "of", "the", "steroid", "molecule", "with", "the", "cytoplasmic", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "resulting", "complex", "appears", "to", "activate", "specific", "gene", "(", "s", ")", "the", "products", "of", "which", "eventually", "cause", "cytolysis", "."]} {"tags": [3, 4, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptors", "in", "lymphoid", "tumors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "is", "a", "range", "of", "levels", "of", "glucocorticoid", "receptor", "numbers", "seen", "in", "the", "various", "subclasses", "of", "acute", "lymphatic", "leukemia", "(", "ALL", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "variability", "can", "not", "be", "explained", "by", "the", "known", "correlation", "between", "active", "cell", "proliferation", "and", "an", "increase", "in", "the", "number", "of", "receptors", ",", "since", "the", "tumors", "with", "the", "highest", "growth", "fraction", "(", "i.e.", ",", "Burkitt", "'s", "lymphoma", "and", "T-cell", "leukemia", ")", "tend", "to", "have", "lower", "average", "receptor", "numbers", "than", "do", "tumors", "with", "lower", "growth", "fractions", "such", "as", "common", "ALL", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["All", "clinical", "specimens", "from", "patients", "with", "lymphatic", "leukemia", "have", "some", "measurable", "level", "of", "glucocorticoid", "receptors", ";", "therefore", ",", "the", "resistance", "seen", "in", "vivo", "can", "not", "be", "explained", "by", "the", "lack", "of", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "there", "has", "been", "a", "positive", "correlation", ",", "in", "our", "hands", ",", "with", "receptor", "level", "and", "prognosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0], "tokens": ["On", "the", "basis", "of", "in", "vitro", "models", ",", "it", "is", "proposed", "that", "perhaps", "the", "high", "receptor", "cell", "lines", "(", "i.e.", ",", "common", "ALL", "of", "childhood", ")", "have", "relative", "stability", "of", "their", "genetic", "material", "making", "glucocorticoid-resistant", "mutations", "less", "likely", "to", "occur", "in", "patients", "with", "these", "cells", "than", "in", "low-receptor", "cell", "lines", "(", "i.e.", ",", "T-cell", "leukemia", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "greater", "genetic", "variability", "in", "the", "low-receptor", "lines", "could", "account", "for", "the", "earlier", "emergence", "of", "clinical", "glucocorticoid", "resistance", "in", "these", "patients", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["[", "Tumor", "histology", "and", "steroid", "receptors", "in", "breast", "carcinoma", "]"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0], "tokens": ["In", "Specimens", "of", "115", "patients", "with", "breast", "cancer", "4", "tumorparameters", "(", "tumorsize", ",", "tumorboder", ",", "nucleargrade", ",", "lymphocytic", "stromal", "reaction", ")", "3", "features", "of", "regional", "lymphnodes", "(", "sinushistiocytosis", ",", "T-cellreaction", ",", "lymphnode", "metastases", ")", "and", "estrogen", "and", "progesteron", "receptors", "were", "determined", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "strong", "sinushistiocytosis", "and", "T-cellreaction", "could", "be", "verified", "mainly", "in", "metastases", "in", "free", "lymphnodes", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "steroid", "receptor", "content", "does", "not", "correlate", "with", "histological", "parameters"]} {"tags": [3, 0, 0, 0, 0], "tokens": ["Aldosterone-receptor", "deficiency", "in", "pseudohypoaldosteronism", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Pseudohypoaldosteronism", ",", "a", "syndrome", "characterized", "by", "salt", "wasting", "and", "failure", "to", "thrive", ",", "usually", "presents", "in", "infancy", "as", "high", "urinary", "levels", "of", "sodium", "despite", "hyponatremia", ",", "hyperkalemia", ",", "hyperreninemia", ",", "and", "elevated", "aldosterone", "levels", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 5, 6, 0], "tokens": ["We", "have", "investigated", "this", "syndrome", "for", "the", "possibility", "of", "abnormal", "Type", "I", "or", "``", "mineralocorticoid-like", "''", "receptors", ",", "which", "have", "intrinsic", "steroid", "specificity", "indistinguishable", "from", "that", "of", "renal", "mineralocorticoid", "receptors", "and", "are", "found", "in", "many", "tissues", "and", "cells", ",", "including", "mononuclear", "leukocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "studied", "three", "patients", "with", "pseudohypoaldosteronism", ":", "the", "28-year-old", "index", "case", "in", "Melbourne", "(", "Patient", "1", ")", "and", "two", "siblings", "in", "Munich", ",", "eight", "and", "two", "years", "of", "age", "(", "Patients", "2", "and", "3", ")", ";", "clinically", ",", "Patient", "3", "had", "a", "less", "severe", "case", "than", "his", "sister", "."]} {"tags": [5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Percoll-separated", "control", "monocytes", "bound", "[", "3H", "]", "aldosterone", "with", "high", "affinity", "(", "Kd", "approximately", "3", "nM", ")", "and", "limited", "capacity", "(", "150", "to", "600", "sites", "per", "cell", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["On", "repeated", "examination", ",", "no", "[", "3H", "]", "aldosterone", "binding", "was", "found", "in", "monocytes", "from", "Patients", "1", "and", "2", ";", "in", "Patient", "3", ",", "the", "levels", "were", "62", "sites", "per", "cell", ",", "more", "than", "2", "S.D.", "below", "those", "of", "the", "control", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Levels", "in", "the", "parents", "of", "the", "Munich", "patients", "(", "first", "cousins", ")", "were", "normal", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["It", "appears", "that", "pseudohypoaldosteronism", "is", "caused", "by", "a", "Type", "I", "receptor", "defect", ",", "that", "the", "defect", "may", "be", "complete", "or", "partial", ",", "that", "transmission", "may", "be", "autosomal", "recessive", ",", "and", "that", "the", "study", "of", "patients", "with", "pseudohypoaldosteronism", "may", "indicate", "physiologic", "roles", "for", "Type", "I", "receptors", "in", "nonepithelial", "tissues", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 7, 8, 8, 0], "tokens": ["Glucocorticoid", "inhibition", "of", "urokinase-like", "plasminogen", "activators", "in", "cultured", "human", "lymphoblasts", "."]} {"tags": [0, 7, 8, 8, 8, 0, 7, 0, 7, 8, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Two", "human", "lymphoblast", "cell", "lines", ",", "LICR-LON-HMy2", "(", "HMy2", "cells", ")", "and", "GM4672A", "cells", ",", "are", "moderately", "growth", "inhibited", "by", "dexamethasone", "(", "1", ",", "4-pregnadien-9-fluoro-16", "alpha-methyl-11", "beta", ",", "17", "alpha", ",", "21-triol-3", ",", "20-dione", ")", "(", "Dex", ")", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 3, 0, 0], "tokens": ["Both", "cell", "types", "secrete", "a", "urokinase", "(", "UK", ")", "-like", "plasminogen", "activator", "(", "PA", ")", "."]} {"tags": [0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Treatment", "of", "both", "HMy2", "and", "GM4672A", "cells", "with", "Dex", "for", "1-4", "days", "inhibits", "extracellular", "PA", "activity", "in", "a", "concentration-dependent", "manner", ",", "being", "half-maximal", "at", "approximately", "1", "X", "10", "(", "-9", ")", "M", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Inhibition", "of", "PA", "in", "both", "cell", "types", "is", "specific", "for", "active", "glucocorticoids", ",", "and", "this", "specificity", "parallels", "the", "ability", "of", "various", "steroids", "to", "bind", "to", "glucocorticoid", "receptors", "."]} {"tags": [7, 8, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0], "tokens": ["HMy2", "cell", "PA", "is", "fully", "suppressible", "by", "Dex", ",", "whereas", "up", "to", "one", "third", "of", "the", "activator", "expressed", "by", "GM4672A", "cells", "is", "resistant", "to", "glucocorticoid", "inhibition", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 3, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Mixing", "experiments", "using", "a", "UK", "standard", "and", "conditioned", "media", "from", "Dex-treated", "cells", "suggest", "an", "absence", "of", "glucocorticoid-inducible", "inhibitors", "to", "UK", "or", "plasmin", "in", "both", "cell", "types", "."]} {"tags": [0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["However", ",", "conditioned", "media", "from", "Dex-treated", "GM4672A", "cells", "inhibits", "a", "portion", "of", "the", "homologous", "cellular", "activator", "in", "conditioned", "media", "from", "control", "GM4672A", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0], "tokens": ["Thus", ",", "low", "levels", "of", "glucocorticoid-inducible", "inhibitors", "may", "contribute", "to", ",", "but", "can", "not", "fully", "account", "for", ",", "Dex", "inhibition", "of", "GM4672A", "PA", "activity", "."]} {"tags": [3, 4, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid-inducible", "inhibitors", "in", "HMy2", "cells", "are", "either", "totally", "absent", "or", "are", "present", "at", "undetectable", "levels", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["Thus", ",", "regulation", "of", "UK-like", "PAs", "in", "HMy2", "and", "GM4672A", "cells", "differs", "with", "respect", "to", "the", "extent", "to", "which", "glucocorticoids", "inhibit", "constitutively", "expressed", "activator", "levels", ",", "as", "well", "as", "the", "possible", "contribution", "of", "glucocorticoid-inducible", "inhibitors", "to", "the", "regulatory", "process", "in", "GM4672A", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Characterization", "of", "aldosterone", "binding", "sites", "in", "circulating", "human", "mononuclear", "leukocytes", "."]} {"tags": [0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Aldosterone", "binding", "sites", "in", "human", "mononuclear", "leukocytes", "were", "characterized", "after", "separation", "of", "cells", "from", "blood", "by", "a", "Percoll", "gradient", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["After", "washing", "and", "resuspension", "in", "RPMI-1640", "medium", ",", "cells", "were", "incubated", "at", "37", "degrees", "C", "for", "1", "h", "with", "different", "concentrations", "of", "[", "3H", "]", "aldosterone", "plus", "a", "100-fold", "concentration", "of", "RU-26988", "(", "11", "alpha", ",", "17", "alpha-dihydroxy-17", "beta-propynylandrost-1", ",", "4", ",", "6-trien-3-one", ")", ",", "with", "or", "without", "an", "excess", "of", "unlabeled", "aldosterone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Aldosterone", "binds", "to", "a", "single", "class", "of", "receptors", "with", "an", "affinity", "of", "2.7", "+/-", "0.5", "nM", "(", "means", "+/-", "SD", ",", "n", "=", "14", ")", "and", "a", "capacity", "of", "290", "+/-", "108", "sites/cell", "(", "n", "=", "14", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "specificity", "data", "show", "a", "hierarchy", "of", "affinity", "of", "desoxycorticosterone", "=", "corticosterone", "=", "aldosterone", "greater", "than", "hydrocortisone", "greater", "than", "dexamethasone", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["The", "results", "indicate", "that", "mononuclear", "leukocytes", "could", "be", "useful", "for", "studying", "the", "physiological", "significance", "of", "these", "mineralocorticoid", "receptors", "and", "their", "regulation", "in", "humans", "."]} {"tags": [3, 4, 4, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Mineralocorticoid", "and", "glucocorticoid", "receptors", "in", "circulating", "mononuclear", "leukocytes", "of", "patients", "with", "primary", "hyperaldosteronism", "."]} {"tags": [3, 4, 4, 4, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mineralocorticoid", "and", "glucocorticoid", "receptors", "were", "measured", "in", "circulating", "mononuclear", "leukocytes", "in", "5", "patients", "affected", "by", "Conn", "'s", "syndrome", "(", "3", "cases", "of", "bilateral", "adrenal", "hyperplasia", "and", "2", "cases", "of", "adenoma", "plus", "unilateral", "hyperplasia", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "number", "of", "the", "binding", "sites", "per", "cell", "resulted", "significantly", "lower", "(", "189", "+/-", "114", ",", "mean", "+/-", "SD", ")", ",", "as", "compared", "with", "the", "normal", "controls", "(", "298", "+/-", "105", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "affinity", "of", "aldosterone", "for", "the", "receptor", "was", "found", "to", "be", "not", "different", "than", "that", "of", "healthy", "control", "subjects", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["The", "capacity", "and", "the", "affinity", "of", "dexamethasone", "for", "glucocorticoid", "receptors", "ranged", "in", "the", "normal", "values", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["These", "data", "suggest", "a", "possible", "down-regulation", "of", "mineralocorticoid", "receptors", "in", "humans", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Short-term", "and", "long-term", "effects", "of", "estrogen", "on", "lymphoid", "tissues", "and", "lymphoid", "cells", "with", "some", "remarks", "on", "the", "significance", "for", "carcinogenesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Estrogens", "have", "long", "been", "thought", "to", "play", "a", "role", "in", "regulating", "the", "immune", "system", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "difference", "in", "some", "types", "of", "immune", "responses", "between", "males", "and", "females", "is", "well-known", ",", "as", "is", "the", "pronounced", "thymic", "involution", "induced", "by", "exogenous", "estrogens", "."]} {"tags": [0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Estrogens", "stimulate", "some", "aspects", "of", "macrophage", "activity", "and", ",", "depending", "on", "dose", "and", "mitogen", ",", "inhibit", "or", "stimulate", "lymphocyte", "proliferative", "response", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Another", "example", "is", "the", "estrogen", "effect", "on", "the", "delayed", "type", "hypersensitivity", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["A", "broad", "review", "is", "given", "of", "such", "estrogen", "effects", "on", "lymphoid", "tissue", "and", "immune", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Most", "of", "the", "studies", "published", "so", "far", "are", "phenomenological", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "recent", "description", "of", "estrogen", "receptors", "in", "the", "thymus", "and", "in", "some", "lymphocyte", "subpopulations", ",", "as", "well", "as", "a", "deeper", "understanding", "of", "regulating", "factors", "in", "the", "immune", "system", ",", "open", "the", "possibility", "of", "a", "more", "detailed", "understanding", "of", "the", "estrogen", "mechanism", "of", "interference", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Estrogen", "effects", "in", "adults", "are", "reversible", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["After", "treating", "neonatal", "mice", "with", "the", "synthetic", "estrogen", "diethylstilbestrol", "(", "DES", ")", ",", "disturbances", "are", "induced", "in", "lymphocyte", "populations", "and", "lymphocyte", "functions", "which", "are", "permanent", "and", "irreversible", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymphocytes", "from", "adult", ",", "neonatally", "DES-treated", "female", "mice", "have", "a", "reduced", "mitogen", "response", "to", "ConA", "and", "LPS", "(", "T", "and", "B", "cell", "mitogen", ")", "and", "the", "delayed", "type", "hypersensitivity", "response", "is", "depressed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0], "tokens": ["A", "detailed", "analysis", "demonstrated", "a", "decreased", "T", "helper", "cell", "population", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "activity", "of", "Natural", "Killer", "cells", "is", "permanently", "reduced", "and", "this", "functional", "impairment", "is", "related", "to", "a", "decreased", "number", "of", "these", "cells", ",", "in", "turn", "determined", "at", "the", "bone", "marrow", "level", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "same", "animals", "have", "an", "increased", "sensitivity", "to", "chemical", "carcinogens", "(", "methylcholanthrene", ")", "and", "they", "spontaneously", "develop", "epithelial", "changes", "in", "the", "uterine", "cervix", "which", "morphologically", "are", "similar", "to", "adenocarcinoma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0], "tokens": ["The", "association", "between", "estrogen-associated", "malignancy", "and", "estrogen", "effects", "in", "lymphocyte", "functions", "deserves", "further", "study", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["Drugs", "affecting", "the", "hormonal", "receptors", "of", "normal", "and", "leukaemic", "peripheral", "leucocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 7, 0, 7, 0, 7, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "authors", "investigated", "the", "behaviour", "of", "steroid", "hormone", "uptake", "in", "leukaemic", "cells", "(", "CML", ",", "CLL", ",", "AML", ",", "ALL", ")", ",", "in", "basal", "conditions", "and", "after", "incubation", "with", "drugs", "which", "modify", "the", "cellular", "concentration", "of", "cAMP", ",", "PGE", "and", "PGF", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "results", "demonstrated", "the", "presence", "in", "leukaemic", "cells", "of", "an", "alteration", "in", "the", "incorporation", "of", "steroid", "hormones", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "alteration", "was", "scarcely", "modified", "by", "incubation", "with", "theophylline", ",", "which", "increases", "cellular", "concentration", "of", "cAMP", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["On", "the", "other", "hand", ",", "it", "was", "moderately", "counteracted", "by", "thioproline", "and", "was", "evidently", "inhibited", "by", "flurbiprofen", ",", "which", "also", "reduced", "cellular", "concentrations", "of", "prostaglandins", ",", "particularly", "PGE2", ",", "with", "the", "exception", "of", "PGF2", "which", "showed", "a", "poor", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 0], "tokens": ["Differences", "were", "observed", "in", "the", "behavior", "of", "hormonal", "uptake", "of", "CML", ",", "in", "contrast", "to", "that", "of", "AML", ",", "CLL", "and", "ALL", "peripheral", "leucocytes", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0], "tokens": ["Human", "breast", "cancer", "and", "impaired", "NK", "cell", "function", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Recent", "advances", "in", "tumor", "immunology", "have", "led", "to", "the", "discovery", "of", "a", "new", "lymphoid", "cell", "with", "unique", "antitumor", "activity", "."]} {"tags": [5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Natural", "killer", "(", "NK", ")", "cells", "form", "an", "antitumor", "surveillance", "system", "and", "appear", "to", "be", "vital", "in", "preventing", "tumor", "growth", "and", "metastasis", "in", "animal", "models", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0], "tokens": ["We", "studied", "NK", "activity", "in", "patients", "with", "benign", "and", "malignant", "breast", "disease", ",", "using", "a", "chromium-51", "release", "microtiter", "cytotoxicity", "assay", "with", "K562", "cells", "as", "targets", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Compared", "with", "benign", "controls", ",", "patients", "with", "malignancies", "had", "significantly", "depressed", "NK", "-mediated", "lysis", "(", "P", "less", "than", "0.01", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "lysis", "in", "those", "with", "advanced", "disease", "(", "stages", "II", ",", "III", ",", "and", "IV", ")", "was", "significantly", "less", "than", "in", "those", "with", "limited", "disease", "(", "stage", "I", ")", "(", "P", "less", "than", "0.01", ")", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NK", "activity", "was", "not", "correlated", "to", "estrogen", "or", "progesterone", "receptor", "states", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Positive", "correlation", "of", "a", "depressed", "natural", "killer", "activity", "with", "the", "extent", "of", "tumor", "spread", "supports", "the", "concept", "of", "an", "NK", "cell", "immune", "surveillance", "system", "in", "breast", "cancer", "and", "emphasizes", "its", "importance", "in", "this", "malignancy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["Immunosuppressive", "effect", "of", "serum", "progesterone", "during", "pregnancy", "depends", "on", "the", "progesterone", "binding", "capacity", "of", "the", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cytotoxic", "activity", "and", "progesterone", "binding", "capacity", "of", "the", "lymphocytes", ",", "together", "with", "serum", "progesterone", "concentrations", ",", "were", "determined", "in", "women", "with", "normal", "pregnancy", "or", "with", "a", "clinical", "diagnosis", "of", "threatened", "abortion", "or", "threatened", "premature", "labour", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "lymphocytes", "of", "women", "with", "threatened", "abortion", "or", "threatened", "premature", "labour", "showed", "significantly", "higher", "cytotoxic", "activity", "(", "P", "less", "than", "0.001", ")", "and", "significantly", "lower", "progesterone", "binding", "capacity", "(", "P", "less", "than", "0.001", ")", "than", "did", "lymphocytes", "obtained", "from", "the", "healthy", "pregnant", "women", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Significant", "inverse", "correlation", "was", "found", "between", "progesterone", "binding", "capacity", "and", "cytotoxic", "activity", "of", "the", "lymphocytes", "(", "P", "less", "than", "0.001", ")", ",", "but", "the", "progesterone", "concentration", "of", "the", "pregnancy", "serum", "appeared", "to", "have", "no", "influence", "on", "the", "other", "two", "parameters", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["The", "findings", "indicate", "that", "intact", "progesterone", "binding", "capacity", "of", "the", "lymphocytes", "is", "an", "essential", "factor", "for", "the", "manifestation", "of", "the", "blocking", "effect", "exerted", "by", "pregnancy", "serum", "on", "lymphocyte", "cytotoxicity", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Serum", "sex", "steroid", "and", "peptide", "hormone", "concentrations", ",", "and", "endometrial", "estrogen", "and", "progestin", "receptor", "levels", "during", "administration", "of", "human", "leukocyte", "interferon", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Five", "normally", "cycling", "healthy", "women", "were", "given", "daily", "subcutaneous", "injections", "of", "human", "leukocyte", "interferon", "(", "3", "X", "10", "(", "6", ")", "units/day", ")", "from", "the", "3rd", "through", "23rd", "day", "of", "the", "menstrual", "cycle", ",", "and", "serum", "steroid", "and", "peptide", "hormone", "concentrations", "monitored", "at", "3-day", "intervals", "during", "the", "treatment", "and", "the", "preceding", "control", "cycle", "."]} {"tags": [0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 3, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Concentrations", "of", "cytosol", "and", "nuclear", "estrogen", "receptors", "(", "ERC", "and", "ERN", ",", "respectively", ")", "and", "progestin", "receptors", "(", "PRC", "and", "PRN", ")", "were", "also", "measured", "from", "endometrial", "biopsies", "taken", "on", "the", "24th", "day", "of", "the", "control", "and", "treatment", "cycle", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "an", "extensive", "monitoring", "of", "clinical", "chemical", "and", "hematological", "tests", "from", "the", "blood", "samples", "were", "performed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Serum", "estradiol", "and", "progesterone", "concentrations", "were", "significantly", "decreased", "during", "the", "treatment", "cycle", ",", "suggesting", "that", "interferon", "interacts", "in", "vivo", "with", "the", "function", "of", "both", "FSH", "and", "LH", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 3, 0, 0], "tokens": ["No", "significant", "changes", "were", "observed", "in", "the", "serum", "peptide", "hormone", "concentrations", "measured", "(", "FSH", ",", "LH", ",", "prolactin", ",", "insulin", ",", "growth", "hormone", "and", "TSH", ")", ";", "neither", "were", "the", "levels", "of", "endometrial", "ERC", ",", "ERN", ",", "PRC", "and", "PRN", "affected", "by", "interferon", "administration", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 5, 0, 0], "tokens": ["As", "expected", ",", "interferon", "administration", "resulted", "in", "decreased", "leukocyte", "counts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "an", "increasing", "tendency", "in", "the", "activities", "of", "serum", "alkaline", "phosphatase", "and", "gamma-glutamyltransferase", "during", "the", "interferon", "therapy", "shows", "that", "interferon", "may", "slightly", "interfere", "with", "the", "liver", "function", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "suggest", "that", "one", "of", "the", "mechanisms", "by", "which", "interferon", "treatment", "may", "affect", "the", "growth", "of", "hormone-dependent", "neoplasms", "could", "be", "the", "interaction", "with", "production", "and/or", "function", "of", "circulating", "hormonal", "compounds", "."]} {"tags": [0, 3, 4, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["[", "Glucocorticoid", "receptor", "level", "in", "the", "blood", "leukocytes", "in", "different", "acute", "diseases", "]"]} {"tags": [0, 0, 3, 4, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Content", "of", "glucocorticoid", "receptors", "in", "cytosol", "of", "blood", "leukocytes", ",", "concentration", "of", "cortisol", "and", "amount", "of", "leukocytes", "in", "blood", "were", "studied", "in", "20", "patients", "with", "acute", "impairments", "within", "the", "second", "day", "of", "the", "disease", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Content", "of", "receptors", "in", "cytosol", "of", "blood", "leukocytes", "was", "studied", "using", "3H-triamcinolone", "acetonide", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Distinct", "increase", "in", "amount", "of", "the", "leukocyte", "glucocorticoid", "receptors", "was", "found", "in", "patients", "with", "poisoning", "by", "dichlorethane", "and", "hypnotic", "drugs", "under", "conditions", "of", "acute", "myocardial", "infarction", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "acute", "pancreatitis", "content", "of", "the", "leukocyte", "receptors", "was", "not", "altered", "as", "compared", "with", "controls", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Concentration", "of", "endogenous", "cortisol", "was", "increased", "in", "blood", "of", "all", "the", "patients", ",", "except", "of", "the", "cases", "of", "acetate", "intoxication", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["Reverse", "correlation", "was", "observed", "between", "concentration", "of", "cortisol", "in", "blood", "and", "content", "of", "glucocorticoid", "receptors", "in", "leukocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["But", "in", "the", "patients", "with", "acute", "pancreatitis", "the", "decrease", "in", "content", "of", "leukocyte", "glucocorticoid", "receptors", "was", "not", "observed", "although", "there", "was", "an", "increase", "in", "cortisol", "concentration", "in", "blood", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5], "tokens": ["The", "role", "of", "glucocorticoid", "receptors", "in", "immunological", "processes", "under", "conditions", "of", "purulent", "complications", "and", "possibility", "to", "regulate", "the", "metabolism", "in", "leukocytes"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Therapeutic", "concentrations", "of", "glucocorticoids", "suppress", "the", "antimicrobial", "activity", "of", "human", "macrophages", "without", "impairing", "their", "responsiveness", "to", "gamma", "interferon", "."]} {"tags": [0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 5, 0, 0, 5, 0], "tokens": ["By", "exposing", "human", "blood-derived", "macrophages", "and", "alveolar", "macrophages", "in", "vitro", "to", "dexamethasone", ",", "we", "showed", "in", "these", "studies", "that", "glucocorticoids", "markedly", "suppress", "the", "antimicrobial", "activity", "of", "macrophages", "but", "not", "macrophage", "activation", "by", "lymphokines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["As", "little", "as", "2.5", "X", "10", "(", "-8", ")", "mol/liter", "of", "dexamethasone", "prevented", "macrophages", "from", "inhibiting", "germination", "of", "Aspergillus", "spores", "or", "from", "eliminating", "ingested", "bacteria", "such", "as", "Listeria", ",", "Nocardia", ",", "or", "Salmonella", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Damage", "to", "macrophage", "function", "was", "inhibited", "by", "progesterone", "and", "appeared", "to", "be", "receptor-mediated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "accordance", "with", "in", "vivo", "observations", ",", "dexamethasone", "required", "24-36", "h", "to", "suppress", "antimicrobial", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["While", "glucocorticoids", "interfered", "with", "base-line", "activity", "of", "macrophages", ",", "dexamethasone", "concentrations", "comparable", "to", "drug", "levels", "in", "patients", "had", "no", "effect", "on", "macrophage", "activation", "."]} {"tags": [0, 5, 0, 3, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Proliferating", "lymphocytes", "and", "gamma-interferon", "thus", "increased", "the", "antimicrobial", "activity", "of", "phagocytes", "exposed", "to", "glucocorticoids", "over", "that", "of", "control", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Macrophage", "activation", "and", "correction", "of", "the", "dexamethasone", "effect", "by", "gamma-interferon", ",", "however", ",", "was", "dependent", "on", "the", "pathogen", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "lymphokine", "enhanced", "the", "antimicrobial", "activity", "of", "dexamethasone-treated", "macrophages", "against", "Listeria", "and", "Salmonella", "but", "not", "against", "Aspergillus", "or", "Nocardia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0], "tokens": ["Dexamethasone-induced", "damage", "to", "the", "antimicrobial", "activity", "of", "human", "macrophages", "in", "vitro", "parallels", "observations", "that", "glucocorticoids", "render", "laboratory", "animals", "susceptible", "to", "listeriosis", "and", "aspergillosis", "by", "damaging", "resident", "macrophages", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Suppression", "of", "macrophage", "antimicrobial", "activity", "should", "thus", "be", "considered", "when", "treating", "patients", "with", "glucocorticoids", ";", "its", "prevention", "by", "gamma-interferon", "might", "be", "beneficial", "for", "some", "but", "not", "all", "pathogens", "."]} {"tags": [3, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["Interleukin", "2", "receptor", "(", "Tac", "antigen", ")", "expression", "in", "HTLV-I-associated", "adult", "T-cell", "leukemia", "."]} {"tags": [3, 0, 3, 0, 0, 0, 3, 0, 0, 0, 5, 0, 0, 0], "tokens": ["Interleukin-2", "(", "IL-2", ")", "is", "a", "lymphokine", "synthesized", "by", "some", "T-cells", "following", "activation", "."]} {"tags": [0, 5, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Resting", "T-cells", "do", "not", "express", "IL-2", "receptors", ",", "but", "receptors", "are", "rapidly", "expressed", "on", "T-cells", "following", "interaction", "of", "antigens", ",", "mitogens", ",", "or", "monoclonal", "antibodies", "with", "the", "antigen-specific", "T-cell", "receptor", "complex", "."]} {"tags": [0, 3, 0, 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "anti-Tac", ",", "a", "monoclonal", "antibody", "that", "recognizes", "the", "IL-2", "receptor", ",", "the", "receptor", "has", "been", "purified", "and", "shown", "to", "be", "a", "Mr", "33", ",", "000", "peptide", "that", "is", "posttranslationally", "glycosylated", "to", "a", "Mr", "55", ",", "000", "mature", "form", "."]} {"tags": [0, 0, 5, 0, 0, 7, 8, 8, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Normal", "resting", "T-cells", "and", "most", "leukemic", "T-cell", "populations", "do", "not", "express", "IL-2", "receptors", ";", "however", ",", "the", "leukemic", "cells", "of", "the", "11", "patients", "examined", "who", "had", "human", "T-cell", "lymphotropic", "virus-associated", "adult", "T-cell", "leukemia", "expressed", "the", "Tac", "antigen", "."]} {"tags": [0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["In", "human", "T-cell", "lymphotropic", "virus-I", "infected", "cells", ",", "the", "Mr", "42", ",", "000", "long", "open", "reading", "frame", "protein", "encoded", "in", "part", "by", "the", "pX", "region", "of", "this", "virus", "may", "act", "as", "a", "transacting", "transcriptional", "activator", "that", "induces", "IL-2", "receptor", "gene", "transcription", ",", "thus", "providing", "an", "explanation", "for", "the", "constant", "association", "of", "IL-2", "receptor", "expression", "with", "adult", "T-cell", "lymphotropic", "virus-I", "infection", "of", "lymphoid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["The", "constant", "expression", "of", "large", "numbers", "of", "IL-2", "receptors", "which", "may", "be", "aberrant", "may", "play", "a", "role", "in", "the", "uncontrolled", "growth", "of", "adult", "T-cell", "leukemia", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Two", "patients", "with", "Tac-positive", "adult", "T-cell", "leukemia", "have", "been", "treated", "with", "the", "anti-Tac", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 4, 0], "tokens": ["One", "of", "the", "patients", "had", "6-", "and", "3-mo", "remissions", "of", "his", "leukemia", "following", "two", "courses", "of", "therapy", "with", "this", "monoclonal", "antibody", "directed", "toward", "this", "growth", "factor", "receptor", "."]} {"tags": [5, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymphocyte", "glucocorticoid", "receptor", "binding", "in", "depressed", "patients", "with", "hypercortisolemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Despite", "elevated", "levels", "of", "serum", "and", "urinary", "cortisol", ",", "patients", "with", "depressive", "illness", "manifest", "none", "of", "the", "clinical", "stigmata", "of", "glucocorticoid", "excess", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["This", "hypercortisolemia", "in", "the", "absence", "of", "clinical", "effects", "suggests", "a", "state", "of", "hormone", "resistance", "and", "could", "be", "mediated", "by", "alterations", "in", "the", "glucocorticoid", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 5, 6, 0], "tokens": ["Earlier", "studies", "have", "shown", "that", "small", "doses", "of", "glucocorticoids", "cause", "a", "decrease", "in", "glucocorticoid", "receptor", "binding", "in", "normal", "human", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["White", "cells", "from", "depressed", "patients", "with", "significant", "hypercortisolemia", "would", "be", "expected", "to", "show", "a", "similar", "change", "in", "receptor", "concentration", "if", "peripheral", "tissues", "are", "adequately", "exposed", "to", "and", "sensitive", "to", "the", "hormone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", "we", "compared", "the", "binding", "of", "[", "3H", "]", "dexamethasone", "to", "lymphocytes", "from", "normal", "subjects", "and", "depressed", "patients", "with", "hypercortisolemia", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymphocytes", "from", "normal", "subjects", "had", "a", "mean", "receptor", "concentration", "of", "10.2", "+/-", "0.66", "fm/10", "(", "6", ")", "cells", "(", "S.E.M.", ")", "and", "a", "dissociation", "constant", "of", "4.8", "+/-", "0.47", "nM", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymphocytes", "from", "depressed", "patients", "with", "abnormal", "0800", "h", "serum", "cortisol", "after", "dexamethasone", "had", "a", "mean", "receptor", "concentration", "of", "8.8", "+/-", "0.75", "fm/10", "(", "6", ")", "cells", ",", "which", "was", "not", "significantly", "different", "from", "that", "in", "lymphocytes", "from", "normal", "subjects", "or", "from", "depressed", "subjects", "with", "normal", "post-dexamethasone", "cortisol", "levels", "(", "9.4", "+/-", "0.95", "fm/10", "(", "6", ")", "cells", ")", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymphocytes", "from", "depressed", "patients", "with", "elevated", "urinary", "free", "cortisol", "excretion", "(", "UFC", ")", "also", "had", "normal", "receptor", "concentration", "and", "binding", "affinity", "for", "dexamethasone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "lack", "of", "a", "change", "in", "lymphocyte", "glucocorticoid", "receptor", "concentration", "in", "the", "presence", "of", "cortisol", "excess", "suggests", "the", "possibility", "that", "hypercortisolemia", "in", "depressive", "illness", "represents", "a", "state", "of", "peripheral", "glucocorticoid", "resistance", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Structure", "and", "regulation", "of", "the", "glucocorticoid", "hormone", "receptor", "."]} {"tags": [0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "glucocorticoid", "receptor", "is", "an", "intracellular", "protein", "which", "possesses", "three", "distinct", "domains", ",", "one", "that", "binds", "agonist", "and", "antagonist", "steroids", ",", "one", "that", "binds", "DNA", ",", "and", "one", "that", "binds", "anti-receptor", "antibodies", "and", "is", "required", "for", "glucocorticoid", "modulation", "of", "gene", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "intact", "cells", ",", "receptor", "number", ",", "affinity", "and", "activity", "can", "change", "in", "response", "to", "factors", "that", "bind", "to", "the", "receptor", ",", "or", "that", "act", "indirectly", "through", "ill-defined", "mechanisms", "which", "may", "include", "resumption", "or", "arrest", "of", "cell", "cycling", "and", "variations", "in", "intracellular", "calcium", "ion", "concentrations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Some", "of", "these", "factors", "appear", "to", "exert", "their", "effect", "by", "controlling", "critical", "receptor", "properties", "such", "as", "ATP-dependent", "phosphorylation", ",", "integrity", "of", "thiol", "groups", ",", "and", "exposure", "of", "key", "amino", "acid", "residues", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "agonists", "promote", "the", "'transformation", "'", "of", "the", "receptor", "into", "the", "DNA-binding", "state", ",", "which", "is", "competent", "for", "modulating", "gene", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Glucocorticoid", "antagonists", "are", "steroids", "that", "interact", "with", "the", "receptor", "but", "either", "fail", "to", "produce", "a", "stable", "complex", "or", "produce", "a", "stable", "but", "inefficient", "complex", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "substituent", "groups", "that", "confer", "agonist", "or", "antagonist", "activity", "to", "the", "steroid", "have", "been", "identified", ",", "the", "molecular", "determinants", "of", "this", "difference", "at", "the", "receptor", "level", "remain", "unknown", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Most", "in", "vitro", "and", "in", "vivo", "data", "on", "receptor", "regulation", "can", "be", "accommodated", "by", "postulating", "the", "existence", "of", "an", "intracellular", "cycle", "that", "involves", "five", "states", "of", "the", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "active", "free", "receptor", "is", "phosphorylated", ",", "reduced", ",", "and", "presumably", "oligomeric", "(", "state", "A", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Following", "binding", "of", "an", "agonist", "(", "state", "B", ")", ",", "it", "can", "become", "transformed", "by", "dissociation", "into", "its", "subunits", "and", "dephosphorylation", "(", "state", "C", ")", "."]} {"tags": [0, 3, 4, 0, 0, 0, 1, 0, 0, 0, 0, 0], "tokens": ["The", "transformed", "receptor", "then", "interacts", "with", "chromatin", "(", "state", "D", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Dissociation", "of", "the", "steroid", "and", "oxidation", "of", "receptor", "thiol", "group", "(", "s", ")", "lead", "to", "the", "inactive", "receptor", "form", "(", "state", "E", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Reduction", "and", "rephosphorylation", "of", "the", "receptor", "enable", "it", "to", "bind", "steroids", "again", "so", "that", "the", "cycle", "is", "closed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Thermodynamics", "of", "steroid", "binding", "to", "the", "human", "glucocorticoid", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["The", "thermodynamics", "of", "the", "interaction", "of", "glucocorticoids", "with", "their", "receptor", "were", "studied", "in", "cytosol", "from", "human", "lymphoblastoid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "rate", "and", "affinity", "constants", "of", "dexamethasone", "and", "cortisol", "between", "0", "degree", "and", "25", "degrees", "C", "were", "calculated", "by", "curve-fitting", "from", "time-course", "and", "equilibrium", "kinetics", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "data", "were", "consistent", "with", "a", "simple", "reversible", "bimolecular", "interaction", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Arrhenius", "and", "Va", "n't", "Hoff", "plots", "were", "curvilinear", "for", "both", "steroids", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["At", "equilibrium", ",", "the", "solution", "for", "the", "equation", "delta", "G", "=", "delta", "H", "-", "T", "X", "delta", "S", "(", "eqn.", "1", ")", "was", "(", "in", "kJ", "X", "mol-1", ")", "-47", "=", "36", "-", "83", "(", "dexamethasone", ")", "and", "-42", "=", "-9", "-", "33", "(", "cortisol", ")", "at", "0", "degree", "C", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Enthalpy", "and", "entropy", "changes", "decreased", "quasi-linearly", "with", "temperature", "such", "that", ",", "at", "25", "degrees", "C", ",", "the", "respective", "values", "were", "-50", "=", "-75", "+", "25", "and", "-43", "=", "-48", "+", "5", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "for", "both", "steroids", ",", "the", "interaction", "was", "entropy-driven", "at", "low", "temperature", "and", "became", "entirely", "enthalpy-driven", "at", "20", "degrees", "C", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thermodynamic", "values", "for", "the", "transition", "state", "were", "calculated", "from", "the", "rate", "constants", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["For", "the", "forward", "reaction", ",", "eqn.", "(", "1", ")", "gave", "45", "=", "84", "-", "39", "(", "dexamethasone", ")", "and", "46", "=", "60", "-", "14", "(", "cortisol", ")", "at", "0", "degree", "C", ",", "and", "44", "=", "24", "+", "20", "(", "dexamethasone", ")", "and", "46", "=", "28", "+", "18", "(", "cortisol", ")", "at", "25", "degrees", "C", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "fit", "quite", "well", "with", "a", "two-step", "model", "[", "Ross", "&", "Subramanian", "(", "1981", ")", "Biochemistry", "20", ",", "3096-3102", "]", "proposed", "for", "ligand-protein", "interactions", ",", "which", "involves", "a", "partial", "immobilization", "of", "the", "reacting", "species", "governed", "by", "hydrophobic", "forces", ",", "followed", "by", "stabilization", "of", "the", "complex", "by", "short-range", "interactions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["On", "the", "basis", "of", "this", "model", ",", "an", "analysis", "of", "the", "transition-state", "thermodynamics", "led", "to", "the", "conclusion", "that", "no", "more", "than", "half", "of", "the", "steroid", "molecular", "area", "is", "engaged", "in", "the", "binding", "process", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Cell", "cycle-related", "changes", "in", "number", "of", "T-lymphocyte", "receptors", "for", "glucocorticoids", "and", "insulin", "."]} {"tags": [0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Enriched", "human", "peripheral", "T-lymphocytes", "were", "stimulated", "with", "PHA", "and", "examined", "for", "variations", "in", "insulin", "and", "glucocorticoid", "(", "dexamethasone", ")", "receptor", "numbers", "during", "the", "early", "phases", "of", "the", "cell", "cycle", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cells", "in", "G0", ",", "G1a", "and", "G1b", "phases", ",", "where", "the", "G1a", "-", "G1b", "transition", "is", "an", "Interleukin", "2", "dependent", "event", ",", "were", "quantitated", "by", "flow", "cytometry", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Few", "but", "significant", "numbers", "of", "glucocorticoid", "receptors", "(", "2700/cell", ")", "and", "no", "insulin", "receptors", "(", "-1/cell", ")", "were", "found", "in", "the", "resting", "(", "G0", ")", "phase", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["As", "cells", "entered", "the", "G1a", "phase", "the", "specific", "binding", "of", "dexamethasone", "increased", "and", "of", "insulin", "took", "place", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "the", "specific", "binding", "further", "increased", "as", "T-cells", "entered", "the", "G1b", "phase", "(", "as", "measured", "at", "44", "h", "of", "incubation", "and", "using", "hydroxyurea-treated", "cells", ")", ",", "the", "major", "changes", "in", "the", "specific", "binding", "of", "dexamethasone", "took", "place", "during", "the", "period", "16", "-", "20", "h", "after", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Based", "on", "these", "findings", ",", "it", "is", "concluded", "that", "both", "receptor", "types", "(", "cell", "membrane", "and", "cytoplasmic", "receptors", ")", "are", "being", "formed", "and", "increased", "at", "G1", "phase", "prior", "to", "cell", "proliferation", ",", "indicating", "the", "importance", "of", "G1", "phase", "in", "immunoregulation", "."]} {"tags": [3, 4, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 7, 0], "tokens": ["Glucocorticoid", "receptors", "and", "cortico-sensitivity", "in", "a", "human", "clonal", "monocytic", "cell", "line", ",", "CM-SM", "."]} {"tags": [7, 0, 0, 7, 8, 0, 5, 6, 6, 6, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CM-SM", "is", "a", "clonal", "line", "of", "human", "precursor", "mononuclear", "phagocytes", "inducible", "to", "macrophage", "differentiation", "in", "response", "to", "the", "tumor", "promoter", "phorbol", "ester", "12-O-tetradecanoyl-phorbol-13-acetate", "(", "TPA", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Untreated", "CM-SM", "cells", "contain", "single", "class", ",", "high-affinity", "(", "KD", "=", "4.0", "X", "10", "(", "-9", ")", "M", ")", "glucocorticoid-specific", "receptor", "sites", "(", "approximately", "60", ",", "000", "per", "cell", ")", ",", "as", "measured", "by", "a", "whole", "cell", "assay", ",", "at", "37", "degrees", "C", ",", "using", "[", "3H", "]", "triamcinolone", "acetonide", "(", "TA", ")", "."]} {"tags": [0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Exposure", "of", "CM-SM", "to", "dexamethasone", "(", "DEX", ")", "produced", "a", "progressive", ",", "dose-", "and", "time-related", "series", "of", "changes", "in", "CM-SM", "cell", "growth", ",", "saturation", "density", ",", "morphology", ",", "and", "functional", "properties", ",", "with", "half-maximal", "effects", "at", "about", "10", "(", "-9", ")", "M", "for", "DEX", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["TA-receptor", "sites", "rapidly", "decreased", "(", "about", "70", "%", ")", "after", "DEX", "treatment", ",", "without", "any", "apparent", "change", "in", "steroid", "specificity", "and", "affinity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["After", "5", "days", "in", "culture", "with", "a", "saturating", "concentration", "(", "3.6", "X", "10", "(", "-8", ")", "M", ")", "of", "hormone", ",", "the", "cells", "reached", "a", "saturation", "density", "of", "about", "9.0", "X", "10", "(", "6", ")", "viable", "cells/ml", "(", "about", "4.0", "X", "10", "(", "6", ")", "viable", "cells/ml", "in", "the", "controls", ")", ",", "while", "the", "modal", "volume", "of", "the", "resulting", "cell", "population", "was", "approximately", "60", "%", ",", "as", "compared", "to", "the", "volume", "of", "untreated", "cells", "."]} {"tags": [7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["DEX-treated", "cells", "appeared", "less", "differentiated", "than", "controls", ",", "as", "assessed", "by", "combined", "morphologic", ",", "antigenic", ",", "and", "cytoenzymatic", "analyses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["DEX", "almost", "completely", "inhibited", "TPA", "activation", "of", "the", "following", "macrophage", "functions", ":", "adherency", "to", "the", "culture", "plate", ",", "expression", "of", "lysosomal", "enzymes", ",", "Fc", "and", "C3", "receptors", ",", "and", "stimulation", "of", "phagocytosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0], "tokens": ["After", "removal", "of", "DEX", ",", "the", "cells", ",", "within", "a", "few", "passages", ",", "returned", "to", "a", "state", "apparently", "identical", "to", "the", "untreated", "controls", "and", "could", "be", "induced", "to", "macrophage", "differentiation", "in", "response", "to", "TPA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Acute", "lymphoblastic", "leukemia", "in", "children", ":", "current", "status", ",", "controversies", ",", "and", "future", "perspective", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Disease-free", "survival", "(", "DFS", ")", "in", "childhood", "ALL", "is", "60", "%", ",", "and", "survival", "in", "good", ",", "average", ",", "and", "poor", "prognostic", "groups", "defined", "by", "initial", "WBC", "and", "age", "is", "90", ",", "60", ",", "and", "45", "%", ",", "respectively", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Additional", "immunological", ",", "morphological", ",", "biochemical", ",", "cytokinetic", ",", "and", "cytogenetic", "factors", "have", "been", "identified", ",", "illustrating", "the", "heterogeneity", "of", "ALL", "and", "its", "derivation", "from", "malignant", "clones", "at", "various", "stages", "of", "differentiation", "and", "with", "varying", "rates", "of", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Of", "biologic", "importance", ",", "these", "factors", "may", "refine", "further", "the", "characteristic", "features", "of", "clinically-determined", "prognostic", "groups", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Multivariate", "analysis", "of", "large", "prospective", "trials", "with", "homogeneous", "therapy", "will", "be", "required", "to", "determine", "the", "independent", "prognostic", "importance", "of", "these", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Current", "treatment", "strategies", "in", "ALL", "include", "(", "1", ")", "tailoring", "therapy", "and", "its", "intensity", "to", "prognostic", "groups", ";", "(", "2", ")", "multiple-drug", "combinations", "in", "induction", ";", "(", "3", ")", "early", "use", "of", "intrathecal", "(", "IT", ")", "methotrexate", "(", "MTX", ")", ";", "(", "4", ")", "CNS", "prophylaxis", "with", "IT", "MTX", "alone", "in", "good", "prognosis", "patients", "and", "combined", "cranial", "radiation", "(", "CXRT", ")", ",", "1800", "rads", "plus", "IT", "MTX", ",", "in", "average", "and", "poor", "prognosis", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Current", "studies", "show", "a", "CNS", "relapse", "rate", "of", "5", "%", "in", "all", "prognostic", "groups", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Late", "neuropsychological", "defects", "caused", "by", "cranial", "XRT", "and", "IT", "MTX", "have", "prompted", "programs", "designed", "to", "reduce", "the", "potential", "late", "toxicity", "of", "CNS", "prophylaxis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["More", "pronounced", "in", "younger", "children", ",", "these", "abnormalities", "include", "decreased", "IQ", ",", "visual-motor", "incoordination", ",", "poor", "performance", "in", "mathematics", ",", "and", "memory", "dysfunction", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Until", "1980", ",", "more", "intensive", "induction", ",", "consolidation", ",", "and", "maintenance", "therapy", "had", "failed", "to", "prolong", "DFS", "in", "children", "with", "a", "poor", "prognosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "West", "Germany", "(", "Berlin-Frankfurt-Muenster", "protocol", ")", "a", "70", "to", "75", "%", "DFS", "is", "seen", "in", "all", "patients", "regardless", "of", "initial", "WBC", ",", "suggesting", "that", "effective", "therapy", "will", "override", "prognostic", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ultra-high-dose", "MTX", ",", "without", "cranial", "radiation", ",", "is", "also", "showing", "promise", "in", "poor", "prognosis", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Other", "issues", "include", "the", "optimal", "duration", "of", "therapy", ",", "the", "role", "of", "testicular", "biopsies", ",", "and", "prophylactic", "testicular", "radiation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Recent", "studies", "suggest", "that", "prognostic", "factors", "lose", "their", "significance", "after", "2", "years", "of", "continuous", "complete", "remission", "and", "that", "2", "years", "of", "maintenance", "therapy", "is", "adequate", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Bilateral", "open-wedge", "testicular", "biopsies", "have", "identified", "occult", "testicular", "disease", "in", "8", "to", "10", "%", "of", "males", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "unified", "approach", "to", "children", "with", "leukemia/lymphoma", ",", "a", "group", "with", "a", "particularly", "poor", "prognosis", ",", "utilizing", "NHL-type", "therapy", "may", "be", "more", "effective", "than", "conventional", "ALL", "therapy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["(", "ABSTRACT", "TRUNCATED", "AT", "400", "WORDS", ")"]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptor", "and", "in", "vitro", "sensitivity", "to", "steroid", "hormones", "in", "human", "lymphoproliferative", "diseases", "and", "myeloid", "leukemia", "."]} {"tags": [0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "glucocorticoid", "receptor", "(", "GR", ")", "quantitation", "by", "a", "whole-cell", "assay", "and/or", "cytosol", "technique", "and", "the", "in", "vitro", "sensitivity", "to", "steroids", "have", "been", "assessed", "in", "peripheral", "blood", "cells", "from", "normal", "donors", "and", "patients", "with", "chronic", "lymphatic", "leukemia", "(", "CLL", ")", ",", "acute", "lymphoblastic", "leukemia", "(", "ALL", ")", ",", "lymphosarcoma", "cell", "leukemia", "(", "LSCL", ")", ",", "acute", "nonlymphatic", "leukemia", "(", "ANLL", ")", ",", "and", "chronic", "myeloid", "leukemia", "(", "CML", ")", "."]} {"tags": [0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Within", "the", "lymphoproliferative", "diseases", ",", "ALL", "cells", "exhibited", "the", "highest", "GR", "concentration", "(", "regardless", "of", "the", "method", "used", ")", "and", "the", "highest", "in", "vitro", "inhibition", "of", "spontaneous", "[", "3H", "]", "thymidine", "(", "[", "3H", "]", "TdR", ")", "uptake", "by", "glucocorticoids", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "significant", "relationship", "between", "GR", "concentration", "(", "whole-cell", "assay", ")", "and", "in", "vitro", "sensitivity", "to", "dexamethasone", "was", "also", "found", "."]} {"tags": [0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["On", "the", "contrary", ",", "CLL", "cells", "presented", "the", "highest", "sensitivity", "to", "glucocorticoids", "in", "PHA-stimulated", "cell", "cultures", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cells", "from", "the", "only", "two", "ALL", "patients", "who", "did", "not", "undergo", "a", "remission", "after", "glucocorticoid-inclusive", "chemotherapy", "had", "both", "the", "lowest", "in", "vitro", "sensitivity", "to", "dexamethasone", "and", "the", "lowest", "GR", "concentration", "with", "whole-cell", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Concerning", "myeloid", "leukemia", ",", "ANLL", "patients", "had", "GR", "concentrations", "slightly", "higher", "than", "those", "found", "in", "the", "ALL", "group", "but", "exhibited", "the", "lowest", "degree", "of", "inhibition", "of", "spontaneous", "[", "3H", "]", "TdR", "uptake", "by", "dexamethasone", "(", "stimulatory", "effects", "occurred", "in", "some", "cases", ")", "."]} {"tags": [7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CML", "cells", "exhibited", "an", "inhibition", "degree", "by", "in", "vitro", "glucocorticoids", "significantly", "higher", "than", "that", "of", "ANLL", "cells", "but", "not", "different", "from", "that", "of", "lymphoproliferative", "diseases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "clear", "relationship", "among", "GR", "pattern", ",", "in", "vitro", "cell", "sensitivity", "to", "glucocorticoids", ",", "and", "clinicohematologic", "parameters", "was", "observed", "in", "myeloid", "leukemia-bearing", "patients", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0], "tokens": ["Glucocorticoid", "receptors", "and", "in", "vitro", "corticosensitivity", "of", "peanut-positive", "and", "peanut-negative", "human", "thymocyte", "subpopulations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "6", "human", "thymus", "glands", ",", "the", "immature", "subset", "of", "thymocytes", "was", "separated", "from", "the", "more", "mature", "one", ",", "by", "differential", "peanut", "lectin", "agglutination", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "2", "cell", "subpopulations", "were", "analyzed", "for", "glucocorticoid", "receptor", "content", "by", "using", "a", "whole", "cell", "assay", ",", "with", "(", "3H", ")", "-triamcinolone", "acetonide", "as", "tracer", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "unagglutinated", "thymocytes", "(", "peanut", "negative", ")", "contained", "about", "2", "times", "more", "receptor", "sites", "per", "cell", "than", "agglutinated", "(", "peanut", "positive", ")", "ones", "(", "7650", "+/-", "1550", "S.D.", "verus", "3195", "+/-", "896", "S.D.", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "affinity", "for", "steroid", "was", "similar", "in", "both", "cell", "subsets", ",", "as", "was", "the", "stereospecificity", "for", "glucocorticoids", ",", "the", "time-course", "of", "steroid-receptor", "association", ",", "and", "cytoplasmic", "to", "nuclear", "translocation", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Despite", "the", "greater", "number", "of", "glucocorticoid", "receptor", "sites", ",", "the", "peanut-negative", "thymocyte", "subpopulation", "did", "not", "differ", "from", "the", "peanut-positive", "one", "in", "its", "sensitivity", "to", "the", "inhibitory", "effects", "of", "triamcinolone", "acetonide", ",", "as", "determined", "by", "measurements", "of", "the", "incorporation", "of", "radiolabeled", "precursors", "of", "protein", "and", "DNA", "."]} {"tags": [0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0], "tokens": ["Moreover", ",", "the", "peanut-negative", "subset", "appeared", "more", "resistant", "in", "vitro", "to", "the", "steroid-induced", "cell", "lysis", "as", "compared", "to", "the", "peanut-positive", "one", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "our", "data", "suggest", "that", "glucocorticoid", "receptor", "density", "and", "corticosensitivity", "are", "not", "directly", "correlated", "and", "that", "the", "number", "of", "glucocorticoid", "receptor", "sites", "may", "be", "dependent", "on", "the", "degree", "of", "immunologic", "maturation"]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Defective", "binding", "and", "function", "of", "1", ",", "25-dihydroxyvitamin", "D3", "receptors", "in", "peripheral", "mononuclear", "cells", "of", "patients", "with", "end-organ", "resistance", "to", "1", ",", "25-dihydroxyvitamin", "D", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lectin-induced", "DNA", "synthesis", "by", "peripheral", "mononuclear", "cells", "from", "17", "normal", "donors", "was", "inhibited", "(", "40-60", "%", ")", "by", "1", ",", "25-dihydroxyvitamin", "D3", "(", "1", ",", "25", "[", "OH", "]", "2D3", ")", "at", "physiological", "concentrations", "(", "10", "(", "-10", ")", "-10", "(", "-9", ")", "M", ")", "."]} {"tags": [0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["The", "lymphocytes", "acquire", "specific", "receptors", "for", "1", ",", "25", "(", "OH", ")", "2D3", "upon", "activation", "by", "the", "lectins", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "process", "precedes", "the", "inhibitory", "effect", "of", "1", ",", "25", "(", "OH", ")", "2D3", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "studied", "lymphocytes", "from", "six", "patients", "from", "four", "different", "kindreds", "with", "the", "syndrome", "of", "hereditary", "end-organ", "resistance", "to", "1", ",", "25", "(", "OH", ")", "2D", "(", "the", "so-called", "vitamin", "D-dependent", "rickets", "type", "II", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "five", "patients", "(", "three", "kindreds", ")", "peripheral", "blood", "mononuclear", "cells", "did", "not", "acquire", "receptors", "for", "1", ",", "25", "(", "OH", ")", "2D3", "upon", "phytohemagglutinin-induced", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "in", "contrast", "to", "normal", "lymphocytes", ",", "the", "mitogenic", "stimulation", "of", "these", "patients", "'", "lymphocytes", "by", "phytohemagglutinin", "and", "concanavalin", "A", "was", "not", "inhibited", "by", "1", ",", "25", "(", "OH", ")", "2D3", "."]} {"tags": [0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Activated", "lymphocytes", "of", "the", "sixth", "patient", "from", "a", "fourth", "kindred", "exhibited", "normal", "binding", "of", "[", "3H", "]", "1", ",", "25", "(", "OH", ")", "2D3", "but", "the", "hormone", "failed", "to", "inhibit", "the", "mitogenic", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "similar", "pattern", "of", "the", "vitamin", "D", "effector", "system", "was", "previously", "observed", "in", "fibroblasts", "cultured", "from", "skin", "biopsies", "of", "the", "same", "group", "of", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "conclusions", "from", "these", "findings", "are", ":", "(", "a", ")", "the", "inhibition", "of", "mitogenic", "stimulation", "by", "1", ",", "25", "(", "OH", ")", "2D3", "is", "mediated", "by", "specific", "functional", "receptors", "to", "the", "hormone", ";", "and", "(", "b", ")", "the", "receptors", "for", "1", ",", "25", "(", "OH", ")", "2D3", "in", "mononuclear", "cells", "are", "probably", "controlled", "genetically", "by", "the", "same", "mechanisms", "as", "the", "effector", "system", "in", "well-characterized", "target", "organs", "of", "the", "hormone", ",", "such", "as", "intestine", "and", "kidney", "."]} {"tags": [3, 4, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptors", "of", "mononuclear", "leukocytes", "from", "myasthenia", "gravis", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 5, 6, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "present", "study", "was", "performed", "to", "analyse", "glucocorticoid", "receptor", "(", "GR", ")", "binding", "in", "peripheral", "blood", "mononuclear", "leukocytes", "(", "MNL", ")", "from", "39", "myasthenia", "gravis", "(", "MG", ")", "patients", "(", "unoperated", "patients", "(", "n", "=", "13", ")", ",", "thymectomized", "patients", "(", "n", "=", "14", ")", "and", "patients", "receiving", "glucocorticoids", ":", "thymectomized", "(", "n", "=", "11", ")", "and", "unoperated", "(", "n", "=", "6", "]", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "whole", "cell", "binding", "assay", "with", "3", "(", "H", ")", "dexamethasone", "was", "used", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["GR", "mean", "values", "were", "significantly", "higher", "in", "the", "MNL", "of", "MG", "patients", "(", "thymectomized", "or", "not", ")", "not", "receiving", "glucocorticoid", "than", "in", "the", "MNL", "of", "healthy", "donors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Affinity", "was", "within", "the", "normal", "range", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Sex", ",", "age", "or", "clinical", "forms", "of", "illness", "did", "not", "influence", "the", "results", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "patients", "receiving", "prednisone", "(", "Pd", ")", "the", "GR", "values", "were", "significantly", "lower", "than", "in", "MG", "patients", "without", "Pd", "therapy", ",", "independent", "of", "Pd", "dose", "or", "time", "of", "administration", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "differences", "in", "receptor", "binding", "between", "normal", "subjects", "and", "MG", "patients", "receiving", "Pd", "have", "been", "found", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Immunological", "interference", "of", "high", "dose", "corticosteroids", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["High-dose", "corticosteroids", "(", "HDC", ")", "will", "influence", "cellular", "as", "well", "as", "humoral", "participants", "of", "the", "immune", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "lymphoid", "tissue", "will", "decrease", "in", "size", "and", "weight", "after", "prolonged", "treatment", "with", "HDC", "."]} {"tags": [0, 0, 0, 0, 0, 0], "tokens": ["Lymphocyte", "functions", "will", "be", "impaired", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0], "tokens": ["Reduced", "synthesis", "of", "B-", "as", "well", "as", "T-lymphocytes", "will", "be", "seen", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "inhibitory", "effect", "on", "B-cell", "function", "can", "be", "observed", "both", "as", "decreased", "serum", "levels", "of", "immunoglobulins", "and", "as", "impaired", "binding", "of", "antibodies", "and", "complement", "to", "the", "cellular", "surface", "."]} {"tags": [0, 5, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0], "tokens": ["Reduced", "T-cell", "function", "indicated", "by", "impaired", "stimulation", "by", "PHA", "and", "porkweed", "as", "well", "as", "by", "impaired", "lymphokinin", "effects", "on", "leukocyte", "migration", "inhibition", "has", "been", "reported", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Reduced", "lymphocyte", "adherence", "to", "antigen", "and", "suppressed", "lymphocyte", "reaction", "have", "also", "been", "observed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Humoral", "factors", "involved", "in", "chemotaxis", ",", "opsonisation", ",", "phagocytosis", ",", "vascular", "permeability", "leading", "to", "leakage", "of", "fluid", "and", "cells", "and", "factors", "involved", "in", "lysis", "of", "antigens", "are", "impaired", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "can", "be", "explained", "partly", "by", "the", "observed", "reduced", "complement", "activation", "via", "the", "alternative", "as", "well", "as", "the", "classical", "pathway", "in", "association", "with", "HDC", "therapy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Acute", "processes", "with", "increased", "vascular", "permeability", "and", "accumulation", "of", "leukocytes", "as", "impairing", "factors", "could", "be", "influenced", "beneficially", "by", "HDC", "therapy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "positive", "effect", "can", "be", "seen", "in", "treatment", "of", "septic", "shock", "or", "rejection", "of", "a", "transplant", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "if", "sepsis", "or", "rejection", "is", "not", "rapidly", "reversed", ",", "complications", "such", "as", "multisystem", "organ", "failure", "and", "bacteremia", "are", "prone", "to", "appear", "."]} {"tags": [0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Identification", "of", "human", "leukemic", "glucocorticoid", "receptors", "using", "affinity", "labeling", "and", "anti-human", "glucocorticoid", "receptor", "antibodies", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 7, 8, 8, 8, 7, 0, 0, 7, 8, 8, 8, 8, 7, 0], "tokens": ["Antisera", "raised", "against", "human", "lymphoid", "glucocorticoid", "receptors", "were", "used", "in", "combination", "with", "the", "glucocorticoid", "receptor", "affinity", "label", "[", "3H", "]", "dexamethasone", "21-mesylate", "[", "(", "3H", "]", "DM", ")", "to", "identify", "the", "glucocorticoid", "receptors", "of", "the", "human", "B-lymphoblastoid", "cell", "line", "IM-9", "and", "the", "human", "T-cell", "leukemic", "cell", "line", "CEM-C7", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Antisera", "were", "obtained", "following", "immunization", "of", "New", "Zealand", "White", "rabbits", "with", "[", "3H", "]", "triamcinolone", "acetonide", "[", "(", "3H", "]", "TA", ")", "-glucocorticoid", "receptor", "complexes", "partially", "purified", "by", "two-stage", "DNA-cellulose", "chromatography", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0], "tokens": ["The", "presence", "of", "anti-human", "glucocorticoid", "receptor", "antibodies", "was", "verified", "by", ":", "(", "a", ")", "adsorption", "of", "[", "3H", "]", "TA-receptor-antibody", "complexes", "to", "Protein", "A", ";", "(", "b", ")", "a", "shift", "to", "higher", "apparent", "molecular", "weight", "in", "the", "elution", "position", "from", "Sephacryl", "S300", "of", "[", "3H", "]", "TA-receptor", "complexes", "incubated", "with", "immune", "serum", ";", "and", "(", "c", ")", "the", "ability", "of", "immune", "serum", "to", "displace", "[", "3H", "]", "TA-receptor", "complexes", "on", "sucrose", "gradients", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["These", "antibodies", "also", "recognized", "rat", "liver", "and", "murine", "S49", "cell", "glucocorticoid", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Sodium", "dodecyl", "sulfate-polyacrylamide", "gel", "electrophoresis", "of", "[", "3H", "]", "DM-labeled", "IM-9", "cytosol", "identified", "a", "major", "competable", "band", "with", "a", "molecular", "weight", "of", "approximately", "90", ",", "000", ",", "three", "minor", "competable", "components", "with", "molecular", "weights", "of", "approximately", "78", ",", "000", ",", "approximately", "51", ",", "000", ",", "and", "approximately", "38", ",", "500", ",", "and", "at", "least", "21", "other", "noncompetable", "components", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Following", "immunoprecipitation", "of", "[", "3H", "]", "DM-labeled", "cytosol", "with", "immune", "serum", ",", "only", "the", "Mr", "90", ",", "000", "and", "78", ",", "000", "components", "were", "seen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Sodium", "dodecyl", "sulfate-polyacrylamide", "gel", "electrophoresis", "of", "[", "3H", "]", "DM-labeled", "CEM-C7", "cytosol", "revealed", "a", "larger", "number", "of", "[", "3H", "]", "DM-labeled", "components", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "after", "immunoprecipitation", "of", "[", "3H", "]", "DM-labeled", "CEM-C7", "cytosol", ",", "a", "predominant", "competable", "component", "with", "a", "molecular", "weight", "of", "90", ",", "000", "was", "easily", "identified", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 7, 0, 0, 0], "tokens": ["This", "component", "was", "markedly", "diminished", "when", "cytosols", "from", "the", "glucocorticoid", "receptor-deficient", "cell", "line", "ICR-27", "were", "used", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Thus", ",", "the", "combination", "of", "affinity", "labeling", "and", "anti-human", "glucocorticoid", "receptor", "antibodies", "is", "capable", "of", "providing", "direct", "physical", "identification", "of", "human", "lymphoid", "glucocorticoid", "receptors", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Effect", "of", "thymosin", "on", "glucocorticoid", "receptor", "activity", "and", "glucocorticoid", "sensitivity", "of", "human", "thymocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Incubation", "with", "thymosin", "fraction", "5", ",", "(", "TMS", "F5", "at", "300", "micrograms/ml", ")", "a", "partially", "purified", "thymic", "factor", ",", "reduced", "the", "steroid", "binding", "activity", "of", "human", "infant", "thymocytes", "from", "9.6", "+/-", "2.1", "fmole/ml", "to", "5.0", "+/-", "2.0", "fmole/ml", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "glucocorticoid", "receptor", "activity", "in", "normal", "infant", "thymocytes", "was", "found", "to", "be", "2", ",", "146", "+/-", "726", "(", "s.d.", ")", "sites", "per", "cell", "with", "dissociation", "constant", "of", "1.4", "+/-", "0.6", "X", "10", "(", "-8", ")", "M", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["TMS", "F5", "also", "increased", "the", "resistance", "of", "human", "thymocytes", "to", "the", "cytolytic", "effect", "of", "dexamethasone", "(", "2.5", "X", "10", "(", "-8", ")", "M", ")", "to", "168.6", "+/-", "30.2", "%", "of", "control", "(", "P", "less", "than", "0.01", ")", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["In", "animals", ",", "medullary", "and", "peripheral", "blood", "T", "cells", "are", "more", "resistant", "to", "glucocorticoids", "than", "immature", "thymic", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "results", "show", "that", "thymosin", "can", "induce", "changes", "consistent", "with", "differentiation", "in", "human", "thymocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "in", "vitro", "results", "are", "consistent", "with", "a", "physiological", "role", "of", "thymosin", "in", "intrathymic", "T", "cell", "maturation", "in", "man", "."]} {"tags": [0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Incubation", "of", "a", "human", "malignant", "thymus", "derived", "T", "cell", "line", "(", "MOLT", "3", ")", "with", "TMS", "F5", "also", "resulted", "in", "a", "significant", "reduction", "of", "the", "number", "of", "steroid", "binding", "sites", "to", "44.2", "+/-", "15.3", "%", "of", "control", "(", "P", "less", "than", "0.05", ")", ",", "but", "TMS", "F5", "did", "not", "significantly", "reduce", "the", "glucocorticoid", "sensitivity", "of", "MOLT", "3", "cells", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 5, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["Specific", "high-affinity", "receptors", "for", "1", ",", "25-dihydroxyvitamin", "D3", "in", "human", "peripheral", "blood", "mononuclear", "cells", ":", "presence", "in", "monocytes", "and", "induction", "in", "T", "lymphocytes", "following", "activation", "."]} {"tags": [5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Human", "peripheral", "blood", "monocytes", "have", "high", "affinity", "binding", "sites", "for", "1", ",", "25-", "(", "OH", ")", "2D3", "(", "Kd", "0.14", "nM", ",", "sedimentation", "coefficient", "3.7S", ")", "."]} {"tags": [5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0], "tokens": ["Resting", "human", "peripheral", "blood", "T", "lymphocytes", ",", "however", ",", "do", "not", "have", "a", "demonstrable", "1", ",", "25-", "(", "OH", ")", "2D3", "receptor", "."]} {"tags": [0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["After", "activation", "with", "phytohemagglutinin", "the", "T", "cells", "exhibit", "the", "receptor", "within", "24", "h", ",", "and", "this", "expression", "is", "blocked", "by", "cycloheximide", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "receptor", "in", "activated", "T", "lymphocytes", "has", "a", "sedimentation", "coefficient", "of", "3.7S", "and", "a", "high", "affinity", "(", "Kd", "0.10", "nM", ")", "for", "the", "ligand", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Effects", "of", "chronic", "glucocorticoid", "excess", "in", "man", "on", "insulin", "binding", "to", "circulating", "cells", ":", "differences", "between", "endogenous", "and", "exogenous", "hypercorticism", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "measured", "[", "125I", "]", "insulin", "binding", "to", "circulating", "monocytes", "or", "erythrocytes", "from", "16", "patients", "with", "chronic", "glucocorticoid", "excess", ",", "9", "chronically", "treated", "with", "prednisone", "and", "7", "with", "adrenocortical", "hyperfunction", "."]} {"tags": [0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["With", "monocytes", ",", "[", "125I", "]", "insulin", "binding", "was", "iincreased", "in", "all", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Analysis", "of", "binding", "data", "indicated", "that", "increased", "binding", "in", "patients", "treated", "with", "prednisone", "was", "due", "to", "an", "ncrease", "in", "receptor", "concentration", ",", "whereas", "in", "patients", "with", "adrenocortical", "hyperfunction", ",", "it", "was", "due", "to", "an", "increase", "in", "receptor", "affinity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["With", "erythrocytes", "from", "patients", "with", "adrenocortical", "hyperfunction", "there", "was", "an", "increase", "in", "receptor", "affinity", "and", "a", "decrease", "in", "receptor", "concentration", ",", "so", "that", "the", "binding", "of", "[", "125I", "]", "insulin", "was", "normal", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "disparity", "of", "results", "between", "endogenous", "and", "exogenous", "hypercorticism", ",", "between", "the", "two", "cell", "types", ",", "and", "between", "the", "present", "studies", "and", "previous", "studies", "suggest", "that", "the", "effects", "of", "glucocorticoid", "excess", "on", "the", "insulin", "receptor", "are", "extremely", "complex", "and", "wide-ranging", "and", "that", "in", "this", "condition", ",", "extrapolations", "in", "humans", "from", "data", "with", "circulating", "cells", "to", "liver", "and", "muscle", "may", "not", "be", "appropriate", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Reduced", "level", "of", "cellular", "glucocorticoid", "receptors", "in", "patients", "with", "anorexia", "nervosa", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Specific", "glucocorticoid", "receptors", "were", "measured", "in", "circulating", "mononuclear", "leukocytes", "from", "12", "patients", "with", "anorexia", "nervosa", "and", "21", "healthy", "control", "subjects", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cells", "from", "patients", "were", "found", "to", "contain", "a", "significantly", "(", "p", "less", "than", "0.01", ")", "lower", "level", "of", "glucocorticoid", "receptor", "(", "3830", "+/-", "210", "sites/cell", ",", "mean", "+/-", "SE", ")", "than", "those", "from", "controls", "(", "4930", "+/-", "250", "sites/cell", ")", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "partial", "glucocorticoid", "receptor", "defect", "may", "well", "explain", "the", "abnormal", "cortisol", "metabolism", "and", "glucocorticoid", "resistance", "commonly", "found", "in", "patients", "with", "anorexia", "nervosa", "."]} {"tags": [0, 0, 0, 3, 4, 0, 5, 6, 0], "tokens": ["Regulation", "of", "the", "glucocorticoid", "receptor", "in", "human", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "presence", "of", "a", "glucocorticoid", "receptor", "in", "human", "lymphocytes", "is", "well", "established", ",", "but", "factors", "affecting", "its", "regulation", "have", "not", "been", "described", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "a", "competitive", "binding", "whole", "cell", "assay", ",", "we", "have", "examined", "the", "binding", "of", "[", "3H", "]", "-dexamethasone", "at", "24", "and", "37", "degrees", "C", "in", "untreated", "normal", "subjects", "and", "in", "healthy", "subjects", "taking", "various", "glucocorticoid", "preparations", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["At", "24", "degrees", "C", "normal", "human", "lymphocytes", "had", "6000", "binding", "sites/cell", "and", "a", "dissociation", "constant", "of", "4", "x", "10", "(", "-9", ")", "M", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "administration", "of", "1", "mg", "of", "dexamethasone", ",", "5", "mg", "of", "prednisone", ",", "and", "37.5", "mg", "of", "cortisone", "acetate", "resulted", "in", "a", "30", "%", "decrease", "in", "binding", "sites", "after", "1", "week", "with", "no", "change", "in", "binding", "affinity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "changes", "in", "the", "number", "of", "binding", "sites", "was", "noted", "before", "1", "week", "and", "the", "diminished", "number", "persisted", "for", "1", "week", "after", "discontinuation", "of", "glucocorticoid", "treatment", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymphocytes", "from", "hospitalized", "patients", "taking", "40-60", "mg", "of", "dexamethasone", "daily", "demonstrated", "the", "same", "change", "in", "number", "of", "binding", "sites", "that", "was", "seen", "in", "normal", "subjects", "taking", "1", "mg", "of", "dexamethasone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["When", "binding", "assays", "were", "carried", "out", "at", "physiologic", "temperature", "there", "was", "the", "same", "decrease", "in", "number", "of", "binding", "sites", "after", "dexamethasone", "administration", ",", "and", "in", "addition", ",", "there", "was", "a", "two-fold", "increase", "in", "binding", "affinity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "administration", "results", "in", "a", "time-dependent", "decrease", "in", "the", "number", "of", "lymphocyte", "glucocorticoid", "binding", "sites", "that", "is", "independent", "of", "the", "type", "of", "glucocorticoid", "administered", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "is", "the", "first", "in", "vivo", "demonstration", "that", "glucocorticoids", "modulate", "their", "own", "receptors", "in", "man", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Immunoglobulin", "localization", "in", "benign", "and", "malignant", "lesions", "of", "the", "human", "mammary", "gland", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "direct", "imunofluorescence", ",", "lesions", "from", "266", "human", "breast", "specimens", "were", "studied", "for", "the", "presence", "of", "IgA", ",", "IgM", ",", "or", "IgG", "localization", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "lesions", "included", "benign", "elements", "from", "66", "subcutaneous", "mastectomy", "specimens", "in", "which", "the", "absence", "of", "simultaneous", "breast", "malignancy", "was", "documented", ",", "primary", "breast", "carcinomas", "from", "153", "mastectomy", "specimens", ",", "and", "47", "biopsies", "containing", "metastatic", "breast", "cancer", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["A", "statistically", "significant", "association", "of", "IgA", "and", "IgM", "with", "benign", "lesions", "was", "contrasted", "to", "the", "association", "of", "IgG", "with", "malignant", "lesions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "both", "primary", "and", "metastatic", "lesions", ",", "IgG", "localization", "was", "associated", "with", "estrogen-receptor-poor", "primary", "cancers", "as", "compared", "with", "estrogen-receptor-rich", "primary", "cancers", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Among", "primary", "breast", "cancer", "patients", ",", "IgG", "localization", "in", "the", "tumor", "correlated", "with", "relative", "lymphopenia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "shorter", "disease-free", "interval", "was", "noted", "in", "association", "with", "IgG", "localization", "among", "the", "metastatic", "breast", "lesions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "statistically", "significant", "association", "between", "stage", "of", "disease", "and", "immunoglobulin", "presence", "was", "demonstrable", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Moderate-to-severe", "intraductal", "epithelial", "hyperplasias", "were", "more", "often", "associated", "with", "immunoglobulin", "G", "localization", "that", "were", "other", "benign", "lesions"]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Correlation", "of", "steroid", "receptors", "with", "histologic", "differentiation", "in", "mammary", "carcinoma", "."]} {"tags": [0, 0, 0, 0], "tokens": ["A", "Singapore", "experience", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cancer", "of", "the", "breast", "is", "the", "most", "common", "tumor", "in", "females", "in", "Singapore", ",", "with", "the", "rate", "of", "20.7", "per", "100", ",", "000", "per", "year", "(", "1977", "estimate", ")", ",", "which", "is", "predicted", "to", "increase", "to", "29.8", "per", "100", ",", "000", "women", "per", "year", "by", "1995", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "detailed", "histopathologic", "review", "of", "50", "primary", "breast", "cancer", "tumors", "analyzed", "for", "estrogen", "receptor", "(", "ER", ")", "level", "was", "carried", "out", "and", "a", "variety", "of", "morphologic", "features", "correlated", "with", "ER", "results", "to", "identify", "any", "factors", "that", "will", "improve", "the", "management", "and", "prognosis", "for", "breast", "cancer", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cytosol", "was", "incubated", "with", "3H-estradiol", "in", "the", "presence", "and", "absence", "of", "cold", "diethylstilbestrol", ",", "and", "bound", "and", "free", "hormone", "were", "separated", "by", "Dextran-coated", "charcoal", "method", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 0, 0], "tokens": ["Tumors", "binding", "more", "than", "5", "fmol/mg", "cytosol", "protein", "were", "classified", "as", "ER", "-positive", "."]} {"tags": [3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Progesterone", "receptor", "(", "PR", ")", "level", "was", "analyzed", "in", "some", "specimens", "with", "the", "use", "of", "a", "similar", "method", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Most", "of", "the", "patients", "were", "Chinese", "(", "90", "%", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Three", "patients", "were", "Malays", ",", "one", "was", "Indian", ",", "and", "one", "was", "European", "in", "this", "series", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Results", "indicated", "that", "there", "was", "strong", "correlation", "between", "ER", "level", ",", "age", ",", "and", "histologic", "grade", "of", "the", "tumors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["No", "correlation", "existed", "between", "absence", "or", "presence", "of", "lymph", "node", "metastases", "and", "ER", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "there", "was", "a", "trend", "for", "ER", "-positive", "tumors", "to", "have", "a", "low-grade", "lymphocytic", "infiltration", ",", "the", "difference", "was", "not", "statistically", "significant", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Mononuclear", "cells", "infiltrating", "human", "mammary", "carcinomas", ":", "immunohistochemical", "analysis", "with", "monoclonal", "antibodies", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 5, 6, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 0, 3, 0, 0], "tokens": ["Breast", "carcinomas", "were", "examined", "by", "the", "immunoperoxidase", "technique", "using", "antisera", "specific", "for", "lymphocyte", "subsets", ",", "monocytes", ",", "NK", "cells", "and", "major", "histocompatibility", "antigens", "(", "HLA-A", ",", "-B", ",", "-C", ";", "Ia-like", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Sixty-four", "per", "cent", "of", "the", "patients", "had", "a", "moderate", "or", "strong", "mononuclear", "cell", "infiltration", ",", "77", "%", "of", "the", "patients", "without", "mononuclear", "cell", "infiltration", "had", "receptors", "for", "estrogens", "as", "compared", "to", "51", "%", "of", "the", "patients", "with", "infiltration", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 0, 5, 6, 0, 0, 0, 7, 8, 0, 0, 0], "tokens": ["The", "majority", "of", "the", "infiltrating", "mononuclear", "cells", "were", "T", "cells", ";", "generally", "the", "OKT8", "cells", "were", "predominant", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "Leu", "3A/OKT8", "cell", "ratio", "was", "not", "related", "to", "histological", "type", ",", "tumor", "size", ",", "age", "of", "the", "patient", "or", "presence", "of", "metastases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Some", "of", "the", "T", "cells", "had", "the", "Ia", "antigen", "and", "were", "thus", "probably", "activated", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "B", "cells", "were", "either", "absent", "or", "less", "numerous", "than", "the", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "was", "no", "relation", "between", "their", "distribution", "and", "the", "various", "parameters", "studied", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["A", "few", "monocytes", "were", "heterogeneous", "according", "to", "their", "markers", "(", "OKM", "I", "and", "acid", "phosphatase", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 3, 4, 0], "tokens": ["In", "6", "cases", "only", "there", "was", "a", "strong", "infiltration", "of", "mononuclear", "cells", "positive", "for", "acid", "phosphatase", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["The", "number", "of", "the", "natural", "killer", "cells", "was", "also", "low", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Only", "a", "few", "mononuclear", "infiltrating", "cells", "had", "receptors", "for", "transferrin", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 5, 6, 0, 0], "tokens": ["There", "was", "a", "positive", "correlation", "between", "the", "inflammatory", "infiltration", "and", "the", "presence", "of", "HLA", "class-I", "antigens", "on", "tumor", "cell", "s", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Some", "of", "the", "antisera", "specific", "for", "lymphocyte", "subsets", "also", "stained", "the", "breast", "carcinoma", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "great", "variations", "in", "the", "subsets", "of", "mononuclear", "cells", "in", "breast", "carcinomas", "may", "correspond", "to", "various", "systems", "of", "defense", "against", "neoplasm", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "case", "of", "male", "pseudohermaphroditism", "with", "normal", "androgen", "receptor", "binding", "and", "47", ",", "XYY", "karyotype", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["A", "case", "of", "male", "pseudohermaphroditism", "with", "47", ",", "XYY", "karyotype", "in", "blood", "and", "cutaneous", "fibroblasts", "is", "described", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "plasma", "testosterone", "response", "to", "HCG", "stimulation", "was", "slightly", "below", "the", "normal", "range", "on", "two", "occasions", "suggesting", "a", "deficit", "of", "gonadal", "function", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "study", "of", "the", "receptors", "for", "dihydrotestosterone", "in", "fibroblasts", "of", "genital", "and", "nongenital", "skin", "showed", "a", "normal", "concentration", "of", "receptors", "in", "genital", "skin", ";", "5-alpha-reductase", "activity", "in", "fibroblasts", "of", "the", "genital", "skin", "was", "low", ",", "but", "the", "plasma", "relationship", "testosterone/dihydrotestosterone", "under", "HCG", "stimulation", "was", "normal", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "diagnostic", "possibility", "of", "a", "complete", "testicular", "feminization", "syndrome", "with", "normal", "receptors", "for", "dihydrotestosterone", "is", "commented", "on", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0, 0], "tokens": ["1", ",", "25-Dihydroxyvitamin", "D3", "inhibits", "antigen-induced", "T", "cell", "activation", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "proliferative", "response", "of", "murine", "spleen", "and", "thymus", "cells", "to", "antigen", "but", "not", "to", "lectin", "was", "inhibited", "by", "the", "active", "metabolite", "of", "vitamin", "D3", ",", "1", ",", "25-", "(", "OH", ")", "2D3", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "directly", "examine", "the", "effect", "of", "1", ",", "25-", "(", "OH", ")", "2D3", "on", "T", "cell", "activation", "in", "the", "absence", "of", "other", "complicating", "interactions", ",", "we", "utilized", "a", "panel", "of", "cloned", "Ia-restricted", "T", "cell", "hybridomas", "that", "secrete", "IL", "2", "on", "activation", "by", "cloned", "Ia-bearing", "stimulator", "cells", "(", "TA3", ")", "or", "when", "stimulated", "by", "mitogen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Physiologic", "concentrations", "of", "1", ",", "25-", "(", "OH", ")", "2D3", "(", "0.01", "to", "0.1", "nm", ")", "inhibited", "the", "antigen-induced", "secretion", "of", "IL", "2", "by", "several", "of", "these", "T", "cell", "hybridomas", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0], "tokens": ["This", "inhibition", "was", "dependent", "on", "the", "concentration", "of", "the", "free", "hormone", "and", "could", "be", "overcome", "by", "increasing", "the", "number", "of", "Ia-bearing", "stimulator", "cells", "used", "."]} {"tags": [0, 0, 0, 7, 8, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Pretreatment", "of", "the", "T", "hybridoma", "but", "not", "the", "TA3", "stimulator", "cell", "with", "1", ",", "25-", "(", "OH", ")", "2D3", "resulted", "in", "inhibition", "of", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["These", "results", "are", "consistent", "with", "the", "finding", "that", "specific", "1", ",", "25-", "(", "OH", ")", "2D3", "receptors", "are", "present", "on", "the", "T", "cell", "hybridomas", "but", "are", "lacking", "in", "TA3", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 3, 0, 0, 0, 3, 4, 0], "tokens": ["1", ",", "25-", "(", "OH", ")", "2D3", "failed", ",", "however", ",", "to", "inhibit", "the", "activation", "of", "the", "T", "cell", "hybridomas", "by", "lectin", "or", "by", "an", "anti-Thy-1", "antibody", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "findings", "suggest", "that", "1", ",", "25-", "(", "OH", ")", "2D3", "may", "be", "interfering", "with", "early", "events", "of", "antigen-induced", "T", "cell", "activation", ",", "perhaps", "by", "hindering", "T", "cell", "recognition", "of", "the", "relevant", "antigen", "on", "stimulator", "cell", "surfaces", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0], "tokens": ["This", "system", "should", "prove", "useful", "in", "studying", "the", "molecular", "mechanisms", "by", "which", "1", ",", "25-", "(", "OH", ")", "2D3", "acts", "to", "inhibit", "T", "cell", "activation", "and", "subsequent", "IL", "2", "production", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptors", "and", "steroid", "sensitivity", "in", "normal", "and", "neoplastic", "human", "lymphoid", "tissues", ":", "a", "review", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "determination", "of", "estrogen", "and", "progesterone", "receptors", "in", "breast", "cancer", "has", "been", "shown", "to", "be", "useful", "in", "predicting", "the", "response", "to", "endocrine", "therapy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Given", "their", "well-known", "inhibitory", "effects", "on", "lymphoid", "tissue", ",", "glucocorticoids", "have", "been", "used", "widely", "in", "the", "treatment", "of", "leukemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Given", "these", "facts", ",", "over", "the", "last", "10", "years", ",", "several", "investigators", "have", "measured", "the", "number", "of", "glucocorticoid", "receptors", "in", "normal", "and", "neoplastic", "lymphoid", "tissue", "to", "see", "whether", "their", "number", "correlated", "with", "glucocorticoid", "responsiveness", "in", "vitro", "or", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "clear", "correlation", "could", "be", "established", "between", "the", "level", "of", "glucocorticoid", "receptor", "and", "the", "in", "vitro", "action", "of", "steroids", "in", "normal", "and", "neoplastic", "lymphoid", "tissue", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "attempts", "to", "correlate", "glucocorticoid", "receptor", "levels", "in", "acute", "lymphocytic", "leukemia", "to", "in", "vivo", "steroid", "responsiveness", "and", "immunological", "type", "using", "the", "whole-cell-binding", "assay", "for", "receptor", "determination", "and", "selecting", "the", "patients", "according", "to", "age", "and", "immunological", "criteria", "have", "been", "more", "successful", "."]} {"tags": [0, 3, 4, 0, 5, 6, 6, 0], "tokens": ["[", "Glucocorticoid", "receptors", "in", "normal", "human", "lymphocytes", "]"]} {"tags": [5, 6, 6, 6, 6, 0, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "(", "GC", ")", "receptors", "were", "studied", "in", "intact", "lymphocytes", "from", "11", "donors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["GC", "binding", "parameters", "were", "found", "to", "be", "highly", "reproducible", "in", "repeated", "experiments", "with", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["It", "was", "shown", "that", "GC", "receptors", "in", "donors", "'", "lymphocytes", "could", "be", "distributed", "into", "two", "different", "classes", "similarly", "to", "the", "pattern", "seen", "in", "skin", "fibroblasts", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Human", "lymphocytes", "are", "an", "adequate", "object", "for", "studying", "genetically", "determined", "variability", "of", "GC", "receptors", "and", "its", "clinical", "importance", "."]} {"tags": [0, 3, 4, 4, 0, 5, 6, 6, 0, 5, 6, 0], "tokens": ["Specific", "estrogen", "binding", "sites", "in", "human", "lymphoid", "cells", "and", "thymic", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "binding", "of", "estrogen", "in", "preparations", "of", "human", "peripheral", "blood", "mononuclear", "cells", ",", "as", "well", "as", "by", "splenic", "and", "thymic", "cells", "is", "demonstrated", "by", "three", "different", "approaches", "(", "Dextran-coated", "charcoal", "method", ",", "whole", "cell", "assay", ",", "and", "gel", "filtration", "on", "a", "sepharose", "4B", "column", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Scatchard", "'s", "analysis", "of", "[", "3H", "]", "-moxestrol", "(", "R2858", ")", "and", "[", "3H", "]", "-estradiol", "binding", "proves", "the", "existence", "of", "a", "single", "class", "of", "receptor", "sites", "having", "a", "dissociation", "constant", "of", "0.18-2.4", "X", "10", "(", "-9", ")", "M", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Physicochemical", "properties", "of", "the", "binder", ",", "including", "binding", "capacity", "and", "steroid", "specificity", ",", "are", "quite", "similar", "to", "those", "reported", "for", "the", "thymus", "of", "small", "mammalian", "species", "or", "human", "thymoma", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Administration", "of", "fibroblast", "interferon", "to", "patients", "with", "advanced", "breast", "cancer", ":", "possible", "effects", "on", "skin", "metastasis", "and", "on", "hormone", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Eleven", "patients", "with", "metastasized", "breast", "cancer", "received", "8", "intramuscular", "injections", "of", "6", "x", "10", "(", "6", ")", "units", "of", "human", "fibroblast", "interferon", "over", "a", "period", "of", "40", "days", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "injections", "did", "not", "cause", "local", "irritation", "or", "inflammation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Fever", "occurred", "in", "only", "1", "of", "the", "11", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "several", "types", "of", "metastases", "were", "monitored", ",", "only", "skin", "nodules", "consistently", "(", "10", "out", "of", "11", "patients", ")", "exhibited", "changes", "that", "were", "suggestive", "of", "a", "therapeutic", "effect", "of", "the", "treatment", "regimen", ":", "either", "a", "simple", "decrease", "in", "size", "of", "some", "nodules", "or", "central", "necrosis", "accompanied", "by", "an", "inflammatory", "reaction", "."]} {"tags": [0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NK-activity", "of", "peripheral", "blood", "leukocytes", "was", "significantly", "increased", "after", "administration", "of", "the", "first", "dose", ";", "the", "effect", "of", "subsequent", "injections", "was", "less", "clear", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Receptors", "for", "estrogens", "and", "progestogens", "were", "increased", "in", "the", "tumor", "biopsies", "of", "2", "out", "of", "2", "and", "5", "out", "of", "6", "patients", "tested", "respectively", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Decreased", "glucocorticoid", "receptor", "binding", "in", "adrenal", "insufficiency", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "examine", "the", "effect", "of", "glucocorticoid", "deficiency", "on", "the", "glucocorticoid", "receptor", ",", "we", "examine", "the", "binding", "of", "[", "3H", "]", "dexamethasone", "to", "lymphocytes", "in", "normal", "subjects", "and", "patients", "with", "adrenal", "insufficiency", "before", "and", "after", "glucocorticoid", "replacement", "therapy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "a", "whole", "cell", "competitive", "binding", "assay", ",", "normal", "human", "lymphocytes", "had", "5977", "+/-", "1487", "(", "mean", "+/-", "SD", ")", "binding", "sites/cell", "and", "a", "dissociation", "constant", "of", "10", "+/-", "2", "nM", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymphocytes", "from", "patients", "with", "untreated", "adrenal", "insufficiency", "had", "fewer", "binding", "sites", "(", "3364", "+/-322", ")", "and", "a", "2-fold", "increase", "in", "binding", "affinity", "(", "5.4", "+/-", "0.9", "mM", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "administration", "of", "conventional", "replacement", "doses", "of", "cortisone", "acetate", "for", "6", "months", "caused", "no", "change", "in", "receptor", "number", ",", "but", "was", "associated", "with", "a", "decrease", "in", "binding", "affinity", "toward", "normal", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["After", "long", "term", "glucocorticoid", "replacement", "therapy", ",", "binding", "parameters", "were", "similar", "to", "those", "in", "patients", "before", "treatment", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "physiological", "implications", "of", "the", "decreased", "receptor", "number", "and", "increased", "binding", "affinity", "in", "adrenal", "insufficiency", "remain", "to", "be", "elucidated", "."]} {"tags": [3, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptor", "concentrations", "and", "terminal", "transferase", "activity", "as", "indicators", "of", "prognosis", "in", "acute", "non-lymphocytic", "leukaemia", "."]} {"tags": [0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Activity", "of", "terminal", "deoxynucleotidyl", "transferase", "(", "TdT", ")", ",", "adenosine", "deaminase", ",", "and", "5'nucleotidase", "and", "the", "cellular", "concentration", "of", "glucocorticoid", "(", "dexamethasone", ")", "receptor", "were", "determined", "in", "25", "patients", "with", "acute", "non-lymphocytic", "leukaemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["All", "patients", "were", "treated", "according", "to", "a", "common", "protocol", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Increased", "activity", "of", "TdT", "(", "greater", "than", "0.1", "unit/microgram", "DNA", ")", "was", "found", "in", "11", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "group", "of", "patients", "was", "shown", "to", "have", "higher", "remission", "and", "survival", "rates", "(", "p", "=", "0.06", ")", "compared", "with", "patients", "with", "low", "activity", "of", "TdT", "."]} {"tags": [0, 3, 4, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "glucocorticoid", "receptor", "concentration", "of", "the", "leukaemic", "blast", "cells", "ranged", "from", "0", "to", "0.94", "fmol/microgram", "DNA", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Thirteen", "patients", "had", "blast", "cells", "with", "a", "glucocorticoid", "receptor", "concentration", "over", "0.22", "fmol/microgram", "DNA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "patients", "had", "significantly", "increased", "remission", "and", "survival", "rates", "(", "p", "=", "0.006", ")", "compared", "with", "those", "with", "a", "low", "receptor", "concentration", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "finding", "can", "not", "be", "explained", "by", "a", "difference", "in", "sensitivity", "to", "glucocorticoids", "since", "these", "were", "not", "used", "as", "therapeutic", "agents", "."]} {"tags": [3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Adenosine", "deaminase", "and", "5'nucleotidase", "activities", "both", "varied", "within", "two", "orders", "of", "magnitude", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "correlation", "could", "be", "found", "between", "activities", "of", "these", "enzymes", "and", "remission", "or", "survival", "rate", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "show", "that", "measurements", "of", "TdT", "activity", "and", "the", "glucocorticoid", "receptor", "concentration", "yield", "valuable", "prognostic", "information", "in", "acute", "non-lymphocytic", "leukaemia"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["[", "3H", "]", "cortivazol", ":", "a", "unique", "high", "affinity", "ligand", "for", "the", "glucocorticoid", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cortivazol", "(", "CVZ", ")", "and", "deacylcortivazol", "(", "DAC", ")", "are", "pyrazolosteroids", "with", "potent", "glucocorticoid", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["In", "previous", "work", "we", "showed", "that", "DAC", "is", "40-fold", "more", "potent", "than", "dexamethasone", "(", "DEX", ")", "in", "lysing", "leukemic", "lymphoblasts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 7, 8, 8, 8, 7, 8, 0], "tokens": ["To", "assess", "the", "interaction", "between", "these", "atypical", "steroids", "and", "the", "glucocorticoid", "receptor", ",", "we", "examined", "the", "binding", "of", "[", "3H", "]", "CVZ", "to", "cytosol", "from", "glucocorticoid-sensitive", "and", "-resistant", "variants", "of", "the", "human", "leukemic", "cell", "line", "CEM", "C7", "."]} {"tags": [0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "glucocorticoid-sensitive", "cells", "[", "3H", "]", "CVZ", "causes", "a", "2-fold", "induction", "of", "glutamine", "synthetase", "and", "binds", "to", "a", "protein", "in", "the", "4.6", "S", "region", "of", "high", "salt", "sucrose", "gradients", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["On", "DEAE-cellulose", "chromatography", ",", "[", "3H", "]", "CVZ-receptor", "complexes", "show", "a", "shift", "from", "high", "(", "0.25", "M", "KP", ")", "to", "low", "salt", "(", "0.09", "M", "KP", ")", "eluting", "forms", "upon", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CVZ", "competes", "for", "a", "97", ",", "000-dalton", "protein", "labeled", "by", "[", "3H", "]", "dexamethasone", "mesylate", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Scatchard", "analysis", "of", "the", "binding", "of", "[", "3H", "]", "CVZ", "in", "glucocorticoid-sensitive", "cells", "revealed", "a", "curvilinear", "plot", "which", "resolved", "into", "high", "(", "0.4", "nM", ")", "and", "low", "(", "11", "nM", ")", "affinity", "components", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["The", "receptor", "concentration", "of", "the", "low", "affinity", "site", "(", "0.30", "pmol/mg", "protein", ")", "was", "approximately", "twice", "that", "of", "the", "high", "affinity", "site", "(", "0.14", "pmol/mg", "protein", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Dissociation", "experiments", "with", "dilution", "and/or", "excess", "unlabeled", "CVZ", "supported", "the", "presence", "of", "independent", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "the", "binding", "of", "[", "3H", "]", "DEX", "to", "C7", "cytosol", "revealed", "a", "single", "class", "of", "binding", "sites", "(", "Kd", "=", "1.9", "nM", ";", "receptor", "concentration", ",", "0.46", "pmol/mg", "protein", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Examination", "of", "the", "binding", "of", "[", "3H", "]", "CVZ", "using", "10", "(", "-5", ")", "M", "DEX", "as", "the", "competing", "ligand", "showed", "that", "DEX", "binds", "only", "to", "the", "low", "affinity", "site", "detected", "by", "[", "3H", "]", "CVZ", "."]} {"tags": [0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 7, 8, 8, 7, 0], "tokens": ["In", "cytosol", "from", "a", "glucocorticoid-resistant", "cell", "line", "with", "virtually", "no", "[", "3H", "]", "DEX", "binding", ",", "[", "3H", "]", "CVZ", "detected", "a", "single", "high", "affinity", "binding", "site", "that", "was", "similar", "in", "dissociation", "constant", "(", "0.8", "nM", ")", "and", "receptor", "concentration", "(", "0.13", "pmol/mg", "protein", ")", "to", "the", "high", "affinity", "site", "detected", "in", "the", "glucocorticoid-sensitive", "cell", "line", "C7", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["A", "controlled", "pore", "glass", "bead", "assay", "for", "the", "measurement", "of", "cytoplasmic", "and", "nuclear", "glucocorticoid", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["An", "assay", "for", "the", "quantitation", "of", "cytoplasmic", "and", "nuclear", "glucocorticoid", "receptors", "in", "lymphoid", "tissue", "has", "been", "developed", "using", "controlled", "pore", "glass", "(", "CPG", ")", "beads", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Soluble", "receptor", "--", "3H-steroid", "complex", "(", "cytosol", "or", "nuclear", "extract", ")", "is", "adsorbed", "quantitatively", "within", "the", "crevasses", "of", "porous", "glass", "beads", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Excess", "labeled", "steroid", "as", "well", "as", "most", "non-specifically", "bound", "steroid", "is", "easily", "washed", "away", ",", "leaving", "the", "hormone-receptor", "complex", "retained", "by", "the", "beads", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Bound", "3H-steroid", "is", "eluted", "with", "ethanol", "and", "measured", "for", "radioactivity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "procedure", "which", "is", "simple", ",", "rapid", ",", "and", "highly", "reproducible", "is", "carried", "out", "using", "frozen", "samples", "(", "stable", "for", "many", "months", ")", "containing", "as", "few", "as", "1", "X", "10", "(", "7", ")", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "comparison", "of", "the", "CPG", "assay", "to", "dextran", "coated", "charcoal", "and", "a", "whole", "cell", "assay", "demonstrates", "that", "CPG", "and", "dextran", "coated", "charcoal", "give", "equivalent", "measurements", "of", "cytosolic", "receptor", "concentration", ",", "while", "the", "CPG", "and", "whole", "cell", "assays", "provide", "equivalent", "values", "for", "total", "receptor", "content", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Plasmacytoid", "blast", "crisis", "in", "B-cell", "chronic", "lymphocytic", "leukemia", ":", "effect", "of", "estradiol", "on", "growth", "and", "differentiation", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 5, 6, 0, 3, 4, 0, 0, 0, 3, 4, 4, 4, 0, 0, 3, 0, 3, 4, 4, 4, 0, 0, 3, 4, 4, 4, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Evolution", "of", "a", "case", "of", "chronic", "lymphocytic", "leukemia", "(", "CLL", ")", "into", "blast", "crisis", "was", "found", "to", "be", "characterized", "by", "three", "unusual", "features", "(", "1", ")", "the", "phenotype", "of", "the", "emerging", "blast", "cells", "was", "that", "of", "pre-plasmacytoid", "cells", "as", "shown", "by", "plasma", "cell", "morphology", "and", "an", "immunological", "phenotype", "corresponding", "partially", "with", "CLL-", "or", "intermediate", "B-cells", ",", "partially", "with", "plasma", "cells", "(", "terminal", "transferase", "-", ",", "common", "acute", "lymphocytic", "leukemia", "antigen", "-", ",", "Ia+", ",", "surface", "immunoglobulin", "heavy", "chains", "-", ",", "surface", "kappa", "light", "chains", "+", ",", "intracytoplasmic", "immunoglobulin", "A+", "and", "G+", ",", "BA-1+", ",", "polyclonal", "gammaglobulin", "production", ")", ";", "(", "2", ")", "cytogenetic", "analysis", "of", "spontaneous", "metaphases", "revealed", "that", "in", "addition", "to", "the", "typical", "CLL", "abnormality", ",", "trisomy", "12", ",", "in", "all", "of", "the", "cells", ",", "an", "additional", "translocation", "between", "chromosomes", "14", "and", "17", "was", "present", "in", "40", "%", "with", "a", "presumptive", "breakpoint", "on", "chromosome", "14", "(", "q12-3", ")", "never", "described", "before", "(", "commonly", "q32", ")", "and", "(", "3", ")", "the", "progression", "of", "the", "disease", "was", "associated", "with", "a", "striking", "increase", "in", "the", "expression", "by", "the", "transformed", "cells", "of", "specific", "binding", "sites", "for", "estradiol", "(", "E2", ")", "due", "to", "an", "actual", "increase", "in", "total", "cellular", "receptor", "proteins", "and", "not", "to", "a", "change", "in", "receptor", "affinity", "for", "E2", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["The", "functional", "status", "of", "the", "steroid", "receptors", "was", "confirmed", "by", "nuclear", "transfer", "of", "the", "cytoplasmic", "hormone-receptor", "complex", "upon", "temperature", "activation", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Since", "the", "rise", "in", "E2-receptor", "display", "paralleled", "a", "large", "increase", "in", "the", "proliferative", "activity", "of", "the", "cells", "as", "well", "as", "a", "change", "in", "their", "maturation", "status", "the", "question", "was", "raised", "as", "to", "whether", "the", "E2-receptor", "should", "be", "considered", "as", "a", "physiological", "marker", "of", "growth", "rate", "or", "of", "cellular", "differentiation", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Exposure", "of", "the", "patient", "'s", "blast", "cells", "to", "E2", "in", "vitro", "resulted", "in", "cessation", "of", "cell", "growth", "following", "at", "least", "one", "mitosis", "after", "addition", "of", "the", "inducer", "as", "seen", "from", "the", "replacement", "of", "the", "large", "blasts", "by", "small", "CLL-like", "cells", "without", "definite", "signs", "of", "alteration", "of", "the", "differentiation", "status", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "suggests", "the", "association", "of", "E2-receptor", "expression", "with", "control", "of", "growth", "rather", "than", "cell", "maturation", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 5, 6, 6, 0, 5, 6, 0], "tokens": ["Multiple", "forms", "and", "fragments", "of", "cytosolic", "glucocorticoid", "receptors", "from", "human", "leukemic", "cells", "and", "normal", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therapy", "with", "glucocorticoids", "is", "generally", "more", "effective", "in", "acute", "lymphoblastic", "leukemia", "than", "in", "other", "types", "of", "human", "leukemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Previous", "studies", ",", "however", ",", "have", "not", "revealed", "any", "consistent", "relationship", "between", "clinical", "responsiveness", "and", "the", "cellular", "or", "cytosolic", "concentration", "of", "glucocorticoid-binding", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["The", "objectives", "of", "this", "study", "were", "to", "determine", "whether", "there", "are", "intrinsic", "structural", "differences", "among", "the", "glucocorticoid", "receptors", "in", "various", "types", "of", "leukemic", "cells", "and", "normal", "lymphocytes", "and", "to", "investigate", "the", "role", "of", "endogenous", "peptidases", "in", "receptor", "degradation", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cytosols", "were", "prepared", "from", "fresh", "or", "rapidly", "frozen", "leukocytes", "from", "6", "healthy", "adults", "and", "35", "high-risk", "leukemia", "patients", "(", "median", "white", "blood", "cell", "count", ",", "150", ",", "000", "cells/microliter", ";", "median", "age", ",", "13", "years", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Receptors", "were", "labeled", "with", "[", "3H", "]", "triamcinolone", "acetonide", "and", "quantitated", "by", "charcoal-dextran", "treatment", "or", "Sephadex", "LH-20", "chromatography", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Mean", "and", "median", "cytosolic", "receptor", "concentrations", "in", "12", "acute", "lymphoblastic", "leukemia", "specimens", "lacking", "the", "standard", "B-cell", "or", "T-cell", "markers", "(", "``", "null", "cells", "''", ")", "were", "approximately", "4-fold", "higher", "than", "in", "23", "other", "leukemic", "cell", "specimens", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "other", "consistent", "differences", "in", "receptor", "content", "were", "observed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Agarose", "filtration", "and", "ultracentrifugation", "in", "hypotonic", "buffers", "containing", "20", "mM", "Na2MoO4", "revealed", "complexes", "of", "similar", "size", "and", "shape", "in", "all", "clinical", "specimens", "tested", "and", "two", "established", "leukemic", "cell", "lines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["They", "had", "Stokes", "radii", "(", "Rs", ")", "of", "8.1", "+/-", "0.5", "(", "S.D.", ")", "nm", "(", "n", "=", "50", ")", ",", "sedimentation", "coefficients", "of", "9.5", "+/-", "0.3S", "(", "n", "=", "40", ")", ",", "molecular", "weights", "of", "approximately", "330", ",", "000", ",", "and", "axial", "ratios", "(", "a/b", ")", "of", "approximately", "12", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "hypertonic", ",", "molybdate-free", "buffer", ",", "these", "oligomeric", "complexes", "were", "dissociated", "into", "subunits", "with", "Rs", "of", "5.9", "+/-", "0.3", "nm", "(", "n", "=", "12", ")", "and", "a/b", "of", "11", "to", "12", ",", "as", "observed", "previously", "for", "other", "receptors", "."]} {"tags": [0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Fragmentation", "of", "the", "oligomer", "and", "the", "subunit", "was", "evident", "in", "some", "cytosols", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["High", "activities", "of", "peptidases", "of", "various", "specificities", "were", "detected", "in", "leukemic", "cell", "cytosols", ",", "as", "in", "other", "cytosols", ",", "by", "fluorometric", "assays", "with", "derivatives", "of", "7-amino-4-methylcoumarin", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Receptor", "cleavage", "by", "these", "and", "other", "endogenous", "enzymes", "may", "account", "for", "previous", "observations", "of", "``", "abnormal", "''", "receptors", "in", "cytosols", "from", "some", "leukemic", "specimens", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "conclude", "that", "intrinsic", "structural", "defects", "in", "the", "receptors", "are", "unlikely", "explanations", "for", "the", "unresponsiveness", "of", "some", "types", "of", "leukemia", "to", "steroid", "therapy", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptor", "number", "and", "intracellular", "water", "space", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "order", "to", "elucidate", "the", "relationship", "between", "cell", "water", "content", "and", "number", "of", "glucocorticoid", "receptors", ",", "eleven", "normal", "and", "malignant", "lymphoid", "or", "myelomonocytic", "cell", "types", "originating", "from", "mouse", ",", "rat", "and", "man", "were", "investigated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "cellular", "water", "space", "was", "measured", "with", "3H2O", ",", "and", "glucocorticoid", "receptor", "number", "was", "measured", "in", "a", "whole-cell", "binding", "assay", "with", "[", "3H", "]", "dexamethasone", "at", "30", "and", "37", "degrees", "C", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0], "tokens": ["The", "intracellular", "water", "phase", "concentration", "of", "glucocorticoid", "receptors", "(", "around", "40", "nmol/l", "cell", "water", ")", ",", "and", "the", "dependence", "of", "receptor", "affinity", "on", "temperature", "were", "similar", "in", "normal", "and", "malignant", "rodent", "and", "human", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0], "tokens": ["It", "is", "concluded", "that", "comparisons", "of", "glucocorticoid", "receptor", "levels", "are", "best", "made", "on", "the", "basis", "of", "intracellular", "receptor", "concentrations", "."]} {"tags": [0, 0, 0, 5, 6, 0, 3, 4, 4, 4, 0], "tokens": ["Covalent", "labeling", "of", "rat", "thymocyte", "and", "human", "lymphoid", "glucocorticoid", "receptor", "."]} {"tags": [5, 6, 0, 0, 3, 0, 0, 0], "tokens": ["Lymphoid", "cells", "contain", "specific", "receptors", "for", "glucocorticoids", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "used", "[", "3H", "]", "dexamethasone-21-mesylate", "to", "label", "covalently", "glucocorticoid", "receptors", "in", "rat", "thymic", "lymphocytes", "and", "in", "neoplastic", "cells", "obtained", "from", "patients", "with", "acute", "lymphoblastic", "leukemia", "and", "malignant", "lymphoma", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "covalently", "labeled", "glucocorticoid", "receptors", "were", "identified", "by", "polyacrylamide", "gel", "electrophoresis", "(", "in", "the", "presence", "of", "0.1", "%", "sodium", "dodecyl", "sulfate", ")", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "cytosolic", "fractions", "prepared", "from", "rat", "thymic", "lymphocytes", ",", "[", "3H", "]", "-dexamethasone-21-mesylate", "labels", "a", "protein", "(", "Mr", "approximately", "equal", "to", "95", ",", "000", ")", "which", "was", "identified", "as", "the", "glucocorticoid", "receptor", "by", "the", "following", "criteria", ":", "(", "a", ")", "labeling", "of", "this", "moiety", "is", "inhibited", "by", "treatment", "with", "a", "100-fold", "molar", "excess", "of", "glucocorticoids", ",", "such", "as", "dexamethasone", "and", "triamcinolone", "acetonide", ";", "and", "(", "b", ")", "the", "covalently", "labeled", "Mr", "approximately", "equal", "to", "95", ",", "000", "protein", "is", "activated", "(", "by", "heating", "at", "20", "degrees", "for", "30", "min", ")", "to", "a", "form", "that", "binds", "to", "DNA-cellulose", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["When", "intact", "thymocytes", "are", "treated", "with", "[", "3H", "]", "dexamethasone-21-mesylate", ",", "an", "Mr", "approximately", "equal", "to", "95", ",", "000", "moiety", "is", "also", "labeled", "covalently", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Approximately", "35", "%", "of", "the", "glucocorticoid", "receptors", "can", "be", "labeled", "covalently", "when", "intact", "thymocytes", "are", "treated", "with", "100", "nM", "[", "3H", "]", "dexamethasone-21-mesylate", "for", "30", "min", "at", "4", "degrees", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Neoplastic", "cells", "from", "acute", "lymphoblastic", "leukemia", "and", "malignant", "lymphoma", "were", "treated", "with", "[", "3H", "]", "dexamethasone-21-mesylate", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "all", "samples", ",", "an", "Mr", "approximately", "equal", "to", "95", ",", "000", "moiety", "was", "labeled", "covalently", ";", "labeling", "was", "inhibited", "by", "excess", "glucocorticoid", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Smaller", "moieties", "were", "also", "identified", "by", "competition", "experiments", ";", "these", "may", "represent", "proteolytic", "fragments", "of", "the", "Mr", "approximately", "equal", "to", "95", ",", "000", "receptor", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Thus", ",", "in", "rat", "and", "human", "lymphoid", "cells", ",", "[", "3H", "]", "dexamethasone-21-mesylate", "can", "be", "used", "to", "label", "covalently", "the", "glucocorticoid", "receptor", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "association", "of", "cytosol", "oestrogen", "and", "progesterone", "receptors", "with", "histological", "features", "of", "breast", "cancer", "and", "early", "recurrence", "of", "disease", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0], "tokens": ["Two", "hundred", "and", "eighty-eight", "primary", "breast", "tumours", "were", "examined", "for", "the", "presence", "or", "absence", "of", "oestrogen", "(", "REc", ")", "and", "progesterone", "(", "RPc", ")", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Analysis", "has", "shown", "a", "relative", "interdependence", "between", "the", "steroid", "receptor", "status", "of", "primary", "breast", "cancer", "and", "other", "prognostic", "variables", "such", "as", "histological", "grade", ",", "lymphocytic", "infiltration", "and", "tumour", "elastosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "were", "significant", "associations", "between", "epithelial", "cellularity", ",", "stromal", "fibrosis", "and", "the", "value", "of", "REc", "in", "those", "tumours", "in", "which", "the", "receptor", "was", "present", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Cellularity", "and", "fibrosis", "were", "unrelated", "to", "the", "presence", "or", "absence", "of", "oestrogen", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["By", "contrast", ",", "neither", "the", "presence", "or", "absence", "nor", "the", "value", "of", "RPc", "could", "be", "related", "to", "cellularity", "or", "fibrosis", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "value", "of", "REc", "and", "RPc", "analysis", "as", "an", "indicator", "of", "prognosis", "was", "examined", "in", "a", "sub-group", "of", "175", "patients", "receiving", "no", "additional", "treatment", "following", "mastectomy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Overall", "relapse-free", "survival", "(", "RFS", ")", "was", "no", "different", "for", "those", "patients", "with", "receptors", "compared", "to", "those", "without", "them", "(", "REc", "P", "=", "0.11", ",", "RPc", "P", "=", "0.7", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "was", "no", "difference", "in", "RFS", "of", "receptor", "positive", "and", "negative", "tumours", "when", "the", "axillary", "node", "status", "was", "taken", "into", "account", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["Clinical", "implications", "of", "glucocorticoid", "receptors", "in", "human", "leukemia", "."]} {"tags": [5, 6, 6, 0, 3, 4, 0], "tokens": ["Normal", "lymphoid", "cells", "contain", "glucocorticoid", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "variety", "of", "stimuli", "that", "activate", "these", "cells", "also", "induce", "increases", "in", "receptor", "concentration", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Similar", "glucocorticoid", "receptors", "can", "be", "detected", "in", "lymphoid", "cells", "from", "patients", "with", "acute", "lymphoblastic", "leukemia", "(", "ALL", ")", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Absence", "of", "the", "glucocorticoid", "receptor", "(", "usually", "found", "in", "treated", "patients", ")", "predicts", "lack", "of", "glucocorticoid", "responsiveness", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "in", "our", "hands", ",", "glucocorticoid", "receptor", "levels", "correlate", "with", "the", "duration", "of", "complete", "remission", "in", "ALL", "(", "though", "not", "in", "other", "forms", "of", "leukemia", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["This", "association", "is", "independent", "of", "cell", "type", ",", "age", ",", "sex", ",", "or", "initial", "leukocyte", "count", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "level", "of", "receptor", "shows", "a", "negative", "correlation", "with", "increasing", "aggressiveness", "of", "the", "tumor", "(", "null-cell", "leukemia", "greater", "than", "T-cell", "leukemia", "greater", "than", "Burkitt", "'s", "lymphoma", ")", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Corticosteroid-mediated", "immunoregulation", "in", "man", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoids", "have", "profound", "and", "complex", "effects", "on", "the", "human", "immune", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "precise", "mechanisms", "of", "the", "corticosteroid-induced", "immunoregulation", "in", "man", "have", "not", "been", "precisely", "defined", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Intracytoplasmic", "corticosteroid-specific", "receptors", "appear", "to", "be", "an", "important", "common", "pathway", "for", "steroid-induced", "changes", ",", "but", "variations", "of", "receptor", "parameters", "do", "not", "account", "for", "the", "multifaceted", "effects", "on", "the", "immune", "system", "."]} {"tags": [5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Human", "circulating", "mononuclear", "cells", "redistribute", "out", "of", "the", "intravascular", "compartment", "following", "treatment", "with", "corticosteroids", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "certain", "components", "at", "this", "redistribution", "phenomenon", "have", "been", "well-characterized", ",", "the", "importance", "of", "this", "compartmental", "cellular", "shift", "with", "respect", "to", "the", "mechanisms", "of", "corticosteroid-induced", "immunoregulation", "are", "less", "well-defined", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Recent", "observations", "that", "activated", "lymphocytes", "may", "be", "sensitive", "to", "the", "lytic", "effects", "of", "glucocorticoids", "suggest", "that", "under", "certain", "situations", "the", "elimination", "of", "selected", "subsets", "of", "cells", "may", "be", "a", "relevant", "mechanism", "of", "corticosteroid-mediated", "immunoregulation", "in", "man", "."]} {"tags": [0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 0], "tokens": ["Corticosteroid-mediated", "effects", "on", "monocyte", "function", "may", "be", "an", "important", "mechanism", "of", "drug-induced", "immunoregulation", "in", "monocyte-dependent", "responses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 0], "tokens": ["In", "some", "experimental", "conditions", ",", "corticosteroids", "inhibit", "Interleukin", "1", "production", "by", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0], "tokens": ["The", "immunoregulatory", "effects", "of", "corticosteroids", "on", "lymphocyte", "immune", "responses", "are", "complex", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "vitro", "corticosteroids", "appear", "to", "selectively", "affect", "early", "immunoregulatory", "events", "as", "opposed", "to", "altering", "an", "established", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0], "tokens": ["Multiple", "sites", "of", "steroid-induced", "modulations", "of", "human", "B", "cell", "responses", "have", "been", "defined", "."]} {"tags": [7, 8, 8, 8, 0, 0, 0, 0, 0], "tokens": ["Human", "lymphoid", "cell", "lines", "and", "glucocorticoids", ":", "II", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0], "tokens": ["Whole", "cell", "and", "cytoplasmic", "binding", "properties", "of", "lymphoblastoid", ",", "leukaemia", "and", "lymphoma", "lines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 7, 0, 0, 0, 0, 0], "tokens": ["The", "glucocorticoid", "binding", "properties", "of", "18", "human", "lymphoid", "cell", "lines", "(", "HLCL", ")", "have", "been", "investigated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "specificity", "of", "steroid", "binding", "was", "confirmed", "with", "various", "glucocorticoid", "agonists", "and", "antagonists", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 7, 0, 0, 7, 0], "tokens": ["A", "gradation", "in", "whole", "cell", "and", "cytoplasmic", "glucocorticoid", "binding", "capacity", "was", "observed", "in", "the", "different", "cell", "line", "types", ":", "lymphoblastoid", "greater", "than", "lymphoma", "greater", "than", "leukaemia", "."]} {"tags": [0, 3, 4, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "cytoplasmic", "receptors", "of", "leukaemia", "and", "lymphoblastoid", "lines", "appeared", "to", "contain", "both", "proteinaceous", "and", "phospholipid", "components", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cytoplasmic", "steroid-receptor", "complexes", "exhibited", "a", "wide", "range", "of", "sedimentation", "coefficients", "(", "8.5-11.3S", ")", "in", "low", "ionic", "strength", "buffer", "but", "there", "was", "no", "correlation", "with", "cell", "line", "type", "or", "glucocorticoid", "sensitivity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Activation", "of", "these", "complexes", "by", "heat", "(", "37", "degrees", "C", ")", "or", "exposure", "to", "high", "ionic", "strength", "buffer", "(", "0.3", "M", "NaCl", ")", "induced", "nuclear", "binding", "of", "steroid", "but", "only", "complexes", "in", "high", "ionic", "strength", "buffer", "manifested", "changes", "in", "sedimentation", "coefficient", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0], "tokens": ["No", "correlation", "was", "observed", "between", "the", "level", "or", "nature", "of", "glucocorticoid", "binding", "and", "the", "cytolethal", "or", "cytostatic", "responsiveness", "of", "HLCL", "to", "glucocorticoid", "treatment", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0], "tokens": ["The", "resistance", "to", "cytolethal", "effects", "can", "not", "be", "ascribed", "to", "a", "failure", "of", "cells", "to", "take", "up", "and", "bind", "steroid", "or", "to", "significant", "differences", "in", "the", "molecular", "species", "of", "cytoplasmic", "receptors", "present", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6], "tokens": ["The", "molecular", "mechanisms", "by", "which", "glucocorticoids", "achieve", "cytolethal", "responses", "in", "human", "lymphoid", "cells"]} {"tags": [3, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0], "tokens": ["CD28", "-mediated", "activation", "in", "CD45RA+", "and", "CD45RO+", "T", "cells", ":", "enhanced", "levels", "of", "reactive", "oxygen", "intermediates", "and", "c-Rel", "nuclear", "translocation", "in", "CD45RA+", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0], "tokens": ["We", "have", "analyzed", "the", "effect", "of", "complete", "T", "cell", "activation", "(", "anti-CD3", "plus", "anti-CD28", ")", "on", "the", "activation", "of", "NF-kappaB", "in", "CD45RA+", "(", "naive", ")", "and", "CD45RO+", "(", "memory/effector", ")", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 5, 6, 0], "tokens": ["Long", "exposure", "(", "24", "h", ")", "induced", "stronger", "NF-kappaB", "DNA", "binding", "in", "CD45RA+", "cells", "than", "in", "CD45RO+", "cells", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0], "tokens": ["Analysis", "of", "the", "nuclear", "c-Rel", "protein", "indicated", "that", "after", "anti-CD3+anti-CD28", "stimulation", "the", "level", "of", "c-Rel", "was", "higher", "in", "CD45RA+", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Analysis", "of", "the", "cytoplasmic", "inhibitor", "IkappaBalpha", "indicated", "that", "anti-CD3+anti-CD28", "stimulation", "induced", "a", "long-lasting", "degradation", "in", "CD45RA+", "cells", "but", "in", "CD45RO+", "cells", "the", "degradation", "process", "was", "more", "rapid", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Because", "the", "CD28", "costimulus", "is", "known", "to", "induce", "the", "production", "of", "reactive", "oxygen", "intermediates", "(", "ROIs", ")", ",", "the", "intracellular", "ROI", "levels", "in", "CD45RA+", "and", "CD45RO+", "cells", "were", "compared", "by", "flow", "cytometry", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["ROIs", "were", "produced", "in", "both", "cell", "types", ",", "but", "more", "strongly", "in", "CD45RA+", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["The", "data", "presented", "in", "this", "study", "further", "emphasize", "the", "differences", "between", "CD45RA+", "and", "CD45RO+", "T", "lymphocytes", "in", "ROI-dependent", "signaling", "pathways", "."]} {"tags": [3, 0, 7, 8, 0, 0, 0, 0], "tokens": ["Ikaros", "in", "hemopoietic", "lineage", "determination", "and", "homeostasis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Studies", "on", "the", "molecular", "mechanisms", "that", "control", "hemopoietic", "differentiation", "have", "focused", "on", "signaling", "cascades", "and", "nuclear", "effectors", "that", "drive", "this", "complex", "developmental", "system", "in", "a", "regulated", "fashion", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "review", "the", "role", "of", "Ikaros", ",", "the", "founding", "member", "of", "a", "unique", "family", "of", "zinc", "finger", "transcription", "factors", "in", "this", "developmental", "process", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["Studies", "on", "an", "Ikaros", "null", "mutation", "have", "revealed", "an", "essential", "role", "for", "this", "factor", "in", "lymphoid", "cell", "fate", "determination", "and", "at", "subsequent", "branch", "points", "of", "the", "T", "cell", "differentiation", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Differences", "in", "the", "phenotypes", "of", "a", "null", "and", "a", "dominant", "negative", "(", "DN", ")", "Ikaros", "mutation", "provide", "insight", "into", "a", "regulatory", "network", "through", "which", "Ikaros", "proteins", "exert", "their", "effects", "in", "development", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", "a", "comparative", "analysis", "of", "the", "hemopoietic", "stem", "cell", "and", "precursor", "compartment", "resulting", "from", "the", "two", "Ikaros", "mutations", "reveals", "a", "profound", "yet", "not", "absolute", "requirement", "for", "Ikaros", "in", "the", "production", "and", "differentiation", "of", "these", "populations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 1, 2, 0], "tokens": ["Overexpression", "of", "p65", "and", "c-Jun", "substitutes", "for", "B7-1", "costimulation", "by", "targeting", "the", "CD28RE", "within", "the", "IL-2", "promoter", "."]} {"tags": [0, 0, 0, 3, 0, 3, 4, 0, 3, 0, 0, 1, 2, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0], "tokens": ["The", "role", "of", "Rel", "and", "activation", "protein-1", "(", "AP-1", ")", "in", "IL-2", "promoter", "activity", "in", "B7-1-", "and", "leukocyte", "function-associated", "Ag-3", "(", "LFA.", "3", ")", "-costimulated", "T", "cells", "has", "been", "evaluated", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 1, 2, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0], "tokens": ["We", "demonstrate", "that", "overexpression", "of", "c-Jun", "but", "not", "c-Fos", "increases", "IL-2", "promoter", "activity", "in", "both", "B7-1-", "and", "LFA-3-costimulated", "Jurkat", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Cotransfection", "of", "both", "c-Jun", "and", "c-Fos", "substitutes", "for", "B7-1", "costimulation", "in", "driving", "an", "activation", "protein-1", "response", "element", "but", "not", "for", "the", "IL-2", "promoter", "."]} {"tags": [0, 0, 3, 4, 0, 0, 7, 8, 8, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 1, 2, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 7, 8, 0], "tokens": ["Overexpression", "of", "Rel", "proteins", "demonstrated", "that", "p65-expressing", "Jurkat", "cells", "transcribed", "equally", "well", "a", "nuclear", "factor", "kappabeta", "reporter", "construct", "when", "costimulated", "with", "B7-1", "or", "LFA-3", ",", "but", "transcription", "of", "IL-2", "promoter", "or", "CD28", "response", "element", "(", "CD28RE", ")", "-driven", "reporters", "was", "superior", "in", "B7-1-costimulated", "cells", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["Combined", "expression", "of", "c-Jun", "and", "p65", "induced", "vigorous", "transcription", "of", "IL-2", "promoter-", "and", "CD28RE-driven", "reporter", "constructs", "in", "both", "LFA-3-", "and", "B7-1-costimulated", "Jurkat", "cells", "."]} {"tags": [0, 0, 1, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 1, 2, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Mutating", "the", "CD28RE", "but", "not", "the", "upstream", "nuclear", "factor", "kappabeta-binding", "site", "in", "the", "IL-2", "promoter", "reduced", "B7-1", "-driven", "transcription", ">", "90", "%", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 1, 2, 0], "tokens": ["The", "results", "implicates", "a", "major", "role", "of", "the", "CD28RE", "in", "the", "integration", "of", "p65", "/", "c-Jun", "-mediated", "transcription", "within", "the", "IL-2", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 1, 0, 0, 0, 1, 2, 0], "tokens": ["We", "suggest", "that", "the", "transition", "from", "an", "autocrine", "LFA-3", "-driven", "immune", "response", "to", "a", "B7", "--", "induced", "paracrine", "immune", "response", "involves", "the", "activation", "of", "c-Jun", "and", "p65", ",", "which", "target", "the", "CD28RE", "region", "of", "the", "IL-2", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Defects", "in", "actin-cap", "formation", "in", "Vav-deficient", "mice", "implicate", "an", "actin", "requirement", "for", "lymphocyte", "signal", "transduction", "."]} {"tags": [0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BACKGROUND", ":", "Antigen-receptor", "interactions", "on", "lymphocytes", "result", "in", "local", "clustering", "of", "actin", ",", "receptors", "and", "signaling", "molecules", "into", "an", "asymmetric", "membrane", "structure", "termed", "a", "cap", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "actin", "polymerization", "is", "known", "to", "be", "required", ",", "the", "mechanisms", "underlying", "cap", "formation", "are", "unclear", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 0], "tokens": ["We", "have", "studied", "the", "events", "underlying", "cap", "formation", "using", "mice", "bearing", "a", "null", "mutation", "in", "vav", "(", "vav", "-/-", ")", ",", "a", "gene", "that", "encodes", "a", "guanine-nucleotide", "exchange", "factor", "for", "the", "GTPase", "Rac", "."]} {"tags": [0, 0, 5, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "Lymphocytes", "from", "vav", "-/-", "mice", "failed", "to", "form", "T-cell", "receptor", "caps", "following", "activation", "and", "had", "a", "defective", "actin", "cytoskeleton", "."]} {"tags": [0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "vav-/-", "T", "cells", "were", "deficient", "in", "interleukin-2", "(", "IL-2", ")", "production", "and", "proliferation", ",", "and", "the", "peak", "of", "Ca2+", "mobilization", "was", "reduced", "although", "of", "normal", "duration", "."]} {"tags": [0, 0, 3, 4, 4, 0, 3, 4, 0, 3, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 1, 2, 2, 2, 0, 0, 0], "tokens": ["Activation", "of", "Jun", "N-terminal", "kinase", "or", "stress-activated", "kinase", "(", "JNK", "or", "SAPK", ")", "and", "mitogen-activated", "protein", "kinase", "(", "MAPK", ")", "and", "the", "induction", "of", "the", "transcription", "factor", "NF-ATc1", "and", "egr-1", "genes", "was", "normal", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Despite", "the", "reduced", "Ca2+", "mobilization", ",", "translocation", "of", "cytoplasmic", "NF-ATc", "to", "the", "nucleus", "was", "normal", ",", "reflecting", "that", "the", "lower", "levels", "of", "Ca2+", "in", "vav-/-", "cells", "were", "still", "sufficient", "to", "activate", "calcineurin", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Treatment", "of", "lymphocytes", "with", "cytochalasin", "D", ",", "which", "blocks", "actin", "polymerization", ",", "inhibited", "cap", "formation", "and", "produced", "defects", "in", "signaling", "and", "IL-2", "transcriptional", "induction", "in", "response", "to", "antigen-receptor", "signaling", "that", "were", "nearly", "identical", "to", "those", "seen", "in", "vav-/-", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["In", "transfection", "studies", ",", "either", "constitutively", "active", "Vav", "or", "Rac", "could", "complement", "constitutively", "active", "calcineurin", "to", "activate", "NF-AT-dependent", "transcription", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["CONCLUSIONS", ":", "These", "results", "indicate", "that", "Vav", "is", "required", "for", "cap", "formation", "in", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "the", "correlation", "between", "cap", "formation", ",", "IL-2", "production", "and", "proliferation", "supports", "the", "hypothesis", "that", "an", "actin-dependent", "pathway", "is", "a", "source", "of", "specialized", "growth", "regulatory", "signals", "."]} {"tags": [3, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["CD14", "-dependent", "activation", "of", "human", "endothelial", "cells", "by", "Bacteroides", "fragilis", "outer", "membrane", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "studied", "the", "capacity", "of", "isolated", "Bacteriodes", "fragilis", "outer", "membrane", ",", "B.", "fragilis", "NCTC9343", "lipopolysaccharide", "(", "LPS", ";", "endotoxin", ")", ",", "and", "B.", "fragilis", "NCTC9343", "capsular", "polysaccharides", "to", "activate", "human", "umbilical", "vein", "endothelial", "cell", "(", "HUVEC", ")", "monolayers", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 1, 2, 0, 0], "tokens": ["To", "assess", "HUVEC", "activation", ",", "E-selectin", "expression", "was", "measured", "by", "enzyme-linked", "immunosorbent", "assay", "(", "ELISA", ")", ",", "Northern", "blot", "analysis", "for", "E-selectin-specific", "mRNA", ",", "and", "electrophoretic", "gel", "mobility", "shift", "assay", "(", "EMSA", ")", "for", "NF-kappa", "B", ",", "a", "transcription", "factor", "necessary", "for", "E-selectin", "gene", "activation", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Exposure", "of", "HUVECs", "to", "B.", "fragilis", "outer", "membrane", "fractions", ",", "separated", "from", "other", "components", "of", "the", "B.", "fragilis", "cell", "wall", "by", "isopycnic", ",", "sucrose", "gradient", "centrifugation", ",", "significantly", "increased", "surface", "expression", "of", "E-selectin", "and", "induced", "functional", "endothelial", "cell", "-dependent", "leukocyte", "adhesion", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0], "tokens": ["B.", "fragilis", "outer", "membranes", "induced", "translocation", "of", "NF-kappa", "B", "to", "HUVEC", "nuclei", "and", "accumulation", "of", "E-selectin", "mRNA", "in", "HUVEC", "cytoplasm", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["E-selectin", "expression", "induced", "by", "B.", "fragilis", "outer", "membranes", "was", "not", "blocked", "by", "polymixin", "B", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "E-selectin", "expression", "induced", "by", "outer", "membrane", "fractions", "purified", "from", "E.", "coli", "was", "competitively", "inhibited", "by", "polymixin", "B", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Neither", "purified", "B.", "fragilis", "LPS", ",", "a", "prominent", "constituent", "of", "the", "outer", "membrane", ",", "nor", "purified", "B.", "fragilis", "capsular", "polysaccharides", "induced", "HUVEC", "activation", "."]} {"tags": [0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Two", "different", "monoclonal", "antibodies", "directed", "against", "human", "CD14", "completely", "inhibited", "B.", "fragilis", "outer", "membrane-induced", "NF-kappa", "B", "activation", ",", "E-selectin", "transcription", ",", "and", "E-selectin", "surface", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["We", "conclude", "that", "the", "outer", "membrane", "component", "of", "the", "B.", "fragilis", "cell", "wall", "contains", "a", "proinflammatory", "factor", "(", "s", ")", ",", "that", "is", "not", "LPS", ",", "which", "induces", "human", "endothelial", "cell", "activation", "by", "a", "soluble", "CD14", "-dependent", "mechanism", "."]} {"tags": [3, 0, 0, 5, 6, 0, 0, 0], "tokens": ["NF-kappaB", "protects", "HIV-1-infected", "myeloid", "cells", "from", "apoptosis", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0], "tokens": ["HIV-1", "infection", "of", "primary", "monocytic", "cells", "and", "myeloid", "cell", "lines", "results", "in", "sustained", "NF-kappaB", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Recently", ",", "NF-kappaB", "induction", "has", "been", "shown", "to", "play", "a", "role", "in", "protecting", "cells", "from", "programmed", "cell", "death", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 8, 8, 8, 0, 0, 0, 0], "tokens": ["In", "the", "present", "study", ",", "we", "sought", "to", "investigate", "whether", "constitutive", "NF-kappaB", "activity", "in", "chronically", "HIV-1-infected", "promonocytic", "U937", "(", "U9-IIIB", ")", "and", "myeloblastic", "PLB-985", "(", "PLB-IIIB", ")", "cells", "affects", "apoptotic", "signaling", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 7, 8, 0], "tokens": ["TNFalpha", "and", "cycloheximide", "caused", "infected", "cells", "to", "undergo", "apoptosis", "more", "rapidly", "than", "parental", "U937", "and", "PLB-985", "cells", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "TNFalpha", "-induced", "NF-kappaB", "activation", "using", "the", "antioxidant", "N-acetylcysteine", "(", "NAC", ")", "resulted", "in", "increased", "apoptosis", "in", "both", "U937", "and", "U9-IIIB", "cells", ",", "while", "preactivation", "of", "NF-kappaB", "with", "the", "non-apoptotic", "inducer", "IL-1beta", "caused", "a", "relative", "decrease", "in", "apoptosis", "."]} {"tags": [0, 0, 0, 3, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "constitutive", "NF-kappaB", "activity", "in", "U9-IIIB", "and", "PLB-IIIB", "cells", "also", "induced", "apoptosis", ",", "suggesting", "that", "NF-kappaB", "protects", "cells", "from", "a", "persistent", "apoptotic", "signal", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["TNFalpha", "plus", "NAC", "treatment", "resulted", "in", "a", "marked", "decrease", "in", "Bcl-2", "protein", "levels", "in", "HIV-1-infected", "cells", ",", "coupled", "with", "an", "increase", "in", "Bax", "protein", "compared", "to", "uninfected", "cells", ",", "suggesting", "that", "the", "difference", "in", "susceptibility", "to", "TNFalpha", "-induced", "apoptosis", "may", "relate", "to", "the", "differences", "in", "relative", "levels", "of", "Bcl-2", "and", "Bax", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["The", "protective", "role", "of", "NF-kappaB", "in", "blocking", "TNFalpha-", "and", "HIV-1-induced", "apoptosis", "was", "supported", "by", "studies", "in", "Jurkat", "T", "cells", "engineered", "to", "express", "IkappaB", "alpha", "repressor", "mutants", "(", "TD-IkappaB", ")", "under", "the", "control", "of", "a", "tetracycline-responsive", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Cells", "underwent", "apoptosis", "in", "response", "to", "TNFalpha", "only", "when", "NF-kappaB", "activation", "was", "inhibited", "by", "TD-IkappaB", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 7, 8, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 6, 0], "tokens": ["As", "was", "observed", "for", "the", "U9-IIIB", "cells", ",", "TNFalpha", "treatment", "also", "induced", "a", "marked", "decrease", "in", "Bcl-2", "protein", "levels", "in", "TD-IkappaB", "expressing", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["These", "experiments", "demonstrate", "that", "apoptotic", "signaling", "is", "perturbed", "in", "HIV-1-infected", "U9-IIIB", "cells", "and", "indicate", "that", "NF-kappaB", "activation", "may", "play", "an", "additional", "protective", "role", "against", "HIV-1-induced", "apoptosis", "in", "myeloid", "cells", "."]} {"tags": [5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Human", "normal", "peripheral", "blood", "B-lymphocytes", "are", "deficient", "in", "DNA-dependent", "protein", "kinase", "activity", "due", "to", "the", "expression", "of", "a", "variant", "form", "of", "the", "Ku86", "protein", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0], "tokens": ["The", "heterodimeric", "Ku", "protein", ",", "which", "comprises", "a", "86", "kDa", "(", "Ku86", ")", "amd", "a", "70", "kDa", "(", "Ku70", ")", "subunits", ",", "is", "an", "abundant", "nuclear", "DNA-binding", "protein", "which", "binds", "in", "vitro", "to", "DNA", "termini", "without", "sequence", "specificity", "."]} {"tags": [3, 0, 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ku", "is", "the", "DNA-targeting", "component", "of", "the", "large", "catalytic", "sub-unit", "of", "the", "DNA-dependent", "protein", "kinase", "complex", "(", "DNA-PK", "[", "CS", "]", ")", ",", "that", "plays", "a", "critical", "role", "in", "mammalian", "double-strand", "break", "repair", "and", "lymphoid", "V", "(", "D", ")", "J", "recombination", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 5, 0, 0, 0], "tokens": ["By", "using", "electrophoretic", "mobility", "shift", "assays", ",", "we", "demonstrated", "that", "in", "addition", "to", "the", "major", "Ku", "x", "DNA", "complex", "usually", "detected", "in", "cell", "line", "extracts", ",", "a", "second", "complex", "with", "faster", "electrophoretic", "mobility", "was", "observed", "in", "normal", "peripheral", "blood", "lymphocytes", "(", "PBL", ")", "extracts", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 5, 6, 0, 0, 5, 6, 6, 0], "tokens": ["The", "presence", "of", "this", "faster", "migrating", "complex", "was", "restricted", "to", "B", "cells", "among", "the", "circulating", "lymphocyte", "population", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Western", "blot", "analysis", "revealed", "that", "B", "cells", "express", "a", "variant", "form", "of", "the", "Ku86", "protein", "with", "an", "apparent", "molecular", "weight", "of", "69", "kDa", ",", "and", "not", "the", "86", "kDa-", "full-length", "protein", "."]} {"tags": [0, 0, 3, 4, 0, 0, 1, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Although", "the", "heterodimer", "Ku70/variant-Ku86", "binds", "to", "DNA-ends", ",", "this", "altered", "form", "of", "the", "Ku", "heterodimer", "has", "a", "decreased", "ability", "to", "recruit", "the", "catalytic", "component", "of", "the", "complex", ",", "DNA-PK", "(", "CS", ")", ",", "which", "contributes", "to", "an", "absence", "of", "detectable", "DNA-PK", "activity", "in", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "provide", "a", "molecular", "basis", "for", "the", "increased", "sensitivity", "of", "B", "cells", "to", "ionizing", "radiation", "and", "identify", "a", "new", "mechanism", "of", "regulation", "of", "DNA-PK", "activity", "that", "operates", "in", "vivo", "."]} {"tags": [0, 0, 5, 6, 6, 6, 6, 0, 3, 0, 3, 4, 4, 0, 0, 0, 3, 0], "tokens": ["Co-stimulation", "of", "human", "peripheral", "blood", "mononuclear", "cells", "with", "IL-2", "and", "anti-CD3", "monoclonal", "antibodies", "induces", "phosphorylation", "of", "CREB", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Phosphorylation", "of", "the", "cAMP-response", "element", "binding", "protein", "CREB", "within", "1", "h", "of", "CD2", "but", "not", "CD3", "cross-linking", "of", "human", "PBMC", "was", "recently", "demonstrated", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0], "tokens": ["The", "absence", "of", "P-CREB", "following", "CD3", "cross-linking", "was", "unexpected", ",", "as", "other", "laboratories", "reported", "increased", "phosphorylation", "of", "CREB", "following", "CD3", "cross-linking", "of", "the", "Jurkat", "lymphocyte", "cell", "line", "."]} {"tags": [0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0], "tokens": ["Due", "to", "Jurkat", "T-cells", "being", "IL-2-independent", ",", "it", "was", "postulated", "that", "IL-2", "might", "provide", "a", "necessary", "co-stimulus", "for", "phosphorylation", "of", "CREB", "in", "primary", "lymphocytes", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0, 0, 3, 4, 4, 4, 4, 4, 0], "tokens": ["Therefore", ",", "P-CREB", "was", "evaluated", "following", "co-stimulation", "of", "human", "PBMC", "through", "the", "IL-2", "and", "CD2", "or", "CD3", "receptors", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["IL-2", "did", "not", "further", "augment", "phosphorylation", "of", "CREB", "following", "CD2", "cross-linking", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "while", "neither", "IL-2", "nor", "CD3", "cross-linking", "alone", "induced", "P-CREB", ",", "a", "4.5-fold", "increase", "in", "phosphorylation", "of", "CREB", "within", "1", "h", "of", "IL-2/CD3", "co-stimulation", "was", "observed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Phosphorylation", "was", "not", "associated", "with", "the", "induction", "of", "cAMP", ",", "and", "inhibition", "of", "PKA", "signaling", "had", "no", "effect", "on", "P-CREB", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Consistent", "with", "signal", "transduction", "through", "p56lck", "or", "p59fyn", ",", "inhibition", "of", "PTK", "signaling", "reduced", "phosphorylation", "50", "%", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interestingly", ",", "inhibiting", "PKC", "signaling", "with", "calphostin", "C", "further", "increased", "P-CREB", "levels", "3-fold", "over", "that", "observed", "in", "IL-2/CD3", "co-stimulated", "cells", "not", "pretreated", "with", "a", "PKC", "inhibitor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", "to", "previous", "studies", "performed", "in", "the", "absence", "of", "exogenous", "IL-2", ",", "no", "increase", "in", "binding", "of", "CREB", "to", "a", "32P-labeled", "oligonucleotide", "probe", "was", "observed", "by", "electrophoretic", "mobility", "shift", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["These", "data", "suggest", "that", "the", "IL-2", "and", "CD3", "signaling", "pathways", "provide", "a", "necessary", "and", "co-operative", "stimulus", "promoting", "phosphorylation", "of", "CREB", "following", "receptor", "cross-linking", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["HIV-1", "infection", "induces", "a", "selective", "reduction", "in", "STAT5", "protein", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["HIV-1", "infection", "is", "accompanied", "by", "qualitative", "and", "quantitative", "defects", "in", "CD4+", "T", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Loss", "of", "immune", "function", "in", "HIV", "patients", "is", "usually", "associated", "with", "a", "profound", "dysregulation", "of", "cytokine", "production", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 0, 0], "tokens": ["To", "investigate", "whether", "cytokine", "signaling", "defects", "occur", "during", "HIV", "infection", ",", "PHA", "blasts", "from", "healthy", "human", "donors", "were", "infected", "with", "two", "strains", "of", "HIV-1", "and", "screened", "for", "the", "expression", "of", "STAT", "proteins", "used", "in", "cytokine", "signaling", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "selective", "decrease", "in", "STAT5B", "was", "seen", "8", "days", "after", "infection", "with", "the", "BZ167", "dual-tropic", "HIV", "isolate", ",", "but", "not", "with", "the", "Ba-L", ",", "M-tropic", "strain", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Based", "on", "these", "findings", ",", "purified", "T", "cells", "from", "HIV-infected", "patients", "in", "different", "stages", "of", "disease", "were", "also", "tested", "for", "STAT", "expression", ";", "decreases", "in", "STAT5A", ",", "STAT5B", ",", "and", "STAT1alpha", "were", "observed", "in", "all", "patients", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["The", "reduction", "in", "STATs", "seen", "in", "vivo", "and", "in", "vitro", "after", "HIV", "infection", "may", "contribute", "to", "the", "loss", "of", "T", "cell", "function", "in", "HIV", "disease", "."]} {"tags": [3, 4, 0, 0, 5, 0, 5, 0, 0], "tokens": ["Transcription", "factors", "that", "regulate", "monocyte", "/", "macrophage", "differentiation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Although", "all", "the", "cells", "in", "an", "organism", "contain", "the", "same", "genetic", "information", ",", "differences", "in", "the", "cell", "phenotype", "arise", "from", "the", "expression", "of", "lineage-specific", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["During", "myelopoiesis", ",", "external", "differentiating", "signals", "regulate", "the", "expression", "of", "a", "set", "of", "transcription", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 5, 0, 5, 0], "tokens": ["The", "combined", "action", "of", "these", "transcription", "factors", "subsequently", "determines", "the", "expression", "of", "myeloid-specific", "genes", "and", "the", "generation", "of", "monocytes", "and", "macrophages", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "particular", ",", "the", "transcription", "factor", "PU.1", "has", "a", "critical", "role", "in", "this", "process", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 0], "tokens": ["We", "review", "the", "contribution", "of", "several", "transcription", "factors", "to", "the", "control", "of", "macrophage", "development"]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transcription", "factor", "LKLF", "is", "sufficient", "to", "program", "T", "cell", "quiescence", "via", "a", "c-Myc", "--", "dependent", "pathway", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["T", "lymphocytes", "circulate", "in", "a", "quiescent", "state", "until", "they", "encounter", "cognate", "antigen", "bound", "to", "the", "surface", "of", "an", "antigen-presenting", "cell", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "molecular", "pathways", "that", "regulate", "T", "cell", "quiescence", "remain", "largely", "unknown", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "show", "that", "forced", "expression", "of", "the", "lung", "Kruppel-like", "transcription", "factor", "(", "LKLF", ")", "in", "Jurkat", "T", "cells", "is", "sufficient", "to", "program", "a", "quiescent", "phenotype", "characterized", "by", "decreased", "proliferation", ",", "reduced", "cell", "size", "and", "protein", "synthesis", "and", "decreased", "surface", "expression", "of", "activation", "markers", "."]} {"tags": [0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Conversely", ",", "LKLF-deficient", "peripheral", "T", "cells", "produced", "by", "gene", "targeting", "showed", "increased", "proliferation", ",", "increased", "cell", "size", "and", "enhanced", "expression", "of", "surface", "activation", "markers", "in", "vivo", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 3, 0], "tokens": ["LKLF", "appeared", "to", "function", ",", "at", "least", "in", "part", ",", "by", "decreasing", "expression", "of", "the", "proto-oncogene", "encoding", "c-Myc", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Forced", "expression", "of", "LKLF", "was", "associated", "with", "markedly", "decreased", "c-Myc", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 0], "tokens": ["In", "addition", ",", "many", "effects", "of", "LKLF", "expression", "were", "mimicked", "by", "expression", "of", "the", "dominant-negative", "MadMyc", "protein", "and", "rescued", "by", "overexpression", "of", "c-Myc", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "LKLF", "is", "both", "necessary", "and", "sufficient", "to", "program", "quiescence", "in", "T", "cells", "and", "functions", ",", "in", "part", ",", "by", "negatively", "regulating", "a", "c-Myc", "--", "dependent", "pathway", "."]} {"tags": [3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0], "tokens": ["HTLV-1", "p12", "(", "I", ")", "protein", "enhances", "STAT5", "activation", "and", "decreases", "the", "interleukin-2", "requirement", "for", "proliferation", "of", "primary", "human", "peripheral", "blood", "mononuclear", "cells", "."]} {"tags": [0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "p12", "(", "I", ")", "protein", ",", "encoded", "by", "the", "pX", "open", "reading", "frame", "I", "of", "the", "human", "T-lymphotropic", "virus", "type", "1", "(", "HTLV-1", ")", ",", "is", "a", "hydrophobic", "protein", "that", "localizes", "to", "the", "endoplasmic", "reticulum", "and", "the", "Golgi", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "p12", "(", "I", ")", "contains", "4", "minimal", "proline-rich", ",", "src", "homology", "3-binding", "motifs", "(", "PXXP", ")", ",", "a", "characteristic", "commonly", "found", "in", "proteins", "involved", "in", "signaling", "pathways", ",", "it", "has", "not", "been", "known", "whether", "p12", "(", "I", ")", "has", "a", "role", "in", "modulating", "intracellular", "signaling", "pathways", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["This", "study", "demonstrated", "that", "p12", "(", "I", ")", "binds", "to", "the", "cytoplasmic", "domain", "of", "the", "interleukin-2", "receptor", "(", "IL-2R", ")", "beta", "chain", "that", "is", "involved", "in", "the", "recruitment", "of", "the", "Jak1", "and", "Jak3", "kinases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 3, 0], "tokens": ["As", "a", "result", "of", "this", "interaction", ",", "p12", "(", "I", ")", "increases", "signal", "transducers", "and", "activators", "of", "transcription", "5", "(", "STAT5", ")", "DNA", "binding", "and", "transcriptional", "activity", "and", "this", "effect", "depends", "on", "the", "presence", "of", "both", "IL-2R", "beta", "and", "gamma", "(", "c", ")", "chains", "and", "Jak3", "."]} {"tags": [0, 0, 7, 8, 8, 8, 8, 8, 0, 5, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 3, 4, 4, 4, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Transduction", "of", "primary", "human", "peripheral", "blood", "mononuclear", "cells", "(", "PBMCs", ")", "with", "a", "human", "immunodeficiency", "virus", "type", "1-based", "retroviral", "vector", "expressing", "p12", "(", "I", ")", "also", "resulted", "in", "increased", "STAT5", "phosphorylation", "and", "DNA", "binding", "."]} {"tags": [0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["However", ",", "p12", "(", "I", ")", "could", "increase", "proliferation", "of", "human", "PBMCs", "only", "after", "stimulation", "of", "T-cell", "receptors", "by", "treatment", "of", "cells", "with", "low", "concentrations", "of", "alphaCD3", "and", "alphaCD28", "antibodies", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["In", "addition", ",", "the", "proliferative", "advantage", "of", "p12", "(", "I", ")", "-transduced", "PBMCs", "was", "evident", "mainly", "at", "low", "concentrations", "of", "IL-2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0], "tokens": ["Together", ",", "these", "data", "indicate", "that", "p12", "(", "I", ")", "may", "confer", "a", "proliferative", "advantage", "on", "HTLV-1-infected", "cells", "in", "the", "presence", "of", "suboptimal", "antigen", "stimulation", "and", "that", "this", "event", "may", "account", "for", "the", "clonal", "proliferation", "of", "infected", "T", "cells", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["(", "Blood.", "2001", ";", "98", ":", "823-829", ")"]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Single", "dose", "intranasal", "administration", "of", "retinal", "autoantigen", "generates", "a", "rapid", "accumulation", "and", "cell", "activation", "in", "draining", "lymph", "node", "and", "spleen", ":", "implications", "for", "tolerance", "therapy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BACKGROUND/AIMS", ":", "A", "single", "intranasal", "delivery", "of", "retinal", "autoantigen", "suppresses", "effectively", "experimental", "autoimmune", "uveoretinitis", "(", "EAU", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "further", "unravel", "underlying", "mechanisms", "the", "authors", "wished", "to", "determine", ",", "firstly", ",", "the", "kinetics", "of", "antigen", "delivery", "and", ",", "secondly", ",", "the", "early", "cellular", "responses", "involved", "in", "the", "initial", "stages", "of", "nasal", "mucosal", "tolerance", "induction", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["METHODS", ":", "Flow", "cytometry", ",", "cell", "proliferation", "assays", ",", "and", "microscopy", "were", "used", "to", "track", "antigen", "following", "a", "single", ",", "intranasal", "dose", "of", "Alexa-488", "labelled", "retinal", "antigen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "A", "rapid", "accumulation", "of", "antigen", "within", "both", "superficial", "cervical", "lymph", "nodes", "(", "SCLN", ")", "and", "spleen", "was", "observed", "after", "30", "minutes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 3, 4, 0, 0, 0], "tokens": ["Significant", "proliferative", "responses", "to", "IRBP", "were", "elicited", "by", "48", "hours", "indicating", "that", "systemic", "priming", "of", "naive", "T", "cells", "to", "retinal", "antigen", "had", "occurred", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cell", "activation", "was", "further", "confirmed", "by", "immunoprecipitation", "studies", ",", "which", "demonstrated", "phosphorylation", "of", "STAT4", "but", "not", "STAT6", "in", "both", "lymph", "nodes", "and", "spleen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "at", "24", "hours", ",", "STAT4", "heterodimerisation", "with", "STAT", "3", "was", "only", "observed", "in", "spleen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["CONCLUSIONS", ":", "The", "results", "provide", "novel", "evidence", "that", "following", "a", "single", "intranasal", "application", "rapid", "transfer", "of", "antigen", "occurs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Resulting", "T", "cell", "proliferation", "develops", "consequent", "to", "differential", "cell", "signalling", "in", "SCLN", "and", "spleen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Further", "understanding", "of", "these", "underlying", "cellular", "mechanisms", ",", "in", "particular", "as", "is", "inferred", "by", "the", "results", "the", "contribution", "of", "local", "versus", "systemic", "tolerance", "induction", ",", "may", "assist", "in", "strategies", "to", "clinically", "apply", "mucosal", "tolerance", "therapy", "successfully", "."]} {"tags": [0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Regulation", "of", "interleukin", "(", "IL", ")", "-18", "receptor", "alpha", "chain", "expression", "on", "CD4", "(", "+", ")", "T", "cells", "during", "T", "helper", "(", "Th", ")", "1/Th2", "differentiation", "."]} {"tags": [0, 0, 0, 0, 3, 0], "tokens": ["Critical", "downregulatory", "role", "of", "IL-4", "."]} {"tags": [3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Interleukin", "(", "IL", ")", "-18", "has", "been", "well", "characterized", "as", "a", "costimulatory", "factor", "for", "the", "induction", "of", "IL-12-mediated", "interferon", "(", "IFN", ")", "-gamma", "production", "by", "T", "helper", "(", "Th", ")", "1", "cells", ",", "but", "also", "can", "induce", "IL-4", "production", "and", "thus", "facilitate", "the", "differentiation", "of", "Th2", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0], "tokens": ["To", "determine", "the", "mechanisms", "by", "which", "IL-18", "might", "regulate", "these", "diametrically", "distinct", "immune", "responses", ",", "we", "have", "analyzed", "the", "role", "of", "cytokines", "in", "the", "regulation", "of", "IL-18", "receptor", "alpha", "chain", "(", "IL-18Ralpha", ")", "expression", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 0, 0, 3, 0], "tokens": ["The", "majority", "of", "peripheral", "CD4", "(", "+", ")", "T", "cells", "constitutively", "expressed", "the", "IL-18Ralpha", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Upon", "antigen", "stimulation", "in", "the", "presence", "of", "IL-12", ",", "marked", "enhancement", "of", "IL-18Ralpha", "expression", "was", "observed", "."]} {"tags": [3, 0, 0, 0, 3, 0, 3, 0], "tokens": ["IL-12", "-mediated", "upregulation", "of", "IL-18Ralpha", "required", "IFN-gamma", "."]} {"tags": [5, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0], "tokens": ["Activated", "CD4", "(", "+", ")", "T", "cells", "that", "expressed", "low", "levels", "of", "IL-18Ralpha", "could", "produce", "IFN-gamma", "when", "stimulated", "with", "the", "combination", "of", "IL-12", "and", "IL-18", ",", "while", "CD4", "(", "+", ")", "cells", "which", "expressed", "high", "levels", "of", "IL-18Ralpha", "could", "respond", "to", "IL-18", "alone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["In", "contrast", ",", "T", "cell", "stimulation", "in", "the", "presence", "of", "IL-4", "resulted", "in", "a", "downregulation", "of", "IL-18Ralpha", "expression", "."]} {"tags": [0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Both", "IL-4", "(", "-/", ")", "-", "and", "signal", "transducer", "and", "activator", "of", "transcription", "(", "Stat", ")", "6", "(", "-/", ")", "-", "T", "cells", "expressed", "higher", "levels", "of", "IL-18Ralpha", "after", "TCR", "stimulation", "."]} {"tags": [0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "activated", "T", "cells", "from", "Stat6", "(", "-/", ")", "-", "mice", "produced", "more", "IFN-gamma", "in", "response", "to", "IL-18", "than", "wild-type", "controls", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "positive/negative", "regulation", "of", "the", "IL-18Ralpha", "by", "the", "major", "inductive", "cytokines", "(", "IL-12", "and", "IL-4", ")", "determines", "the", "capacity", "of", "IL-18", "to", "polarize", "an", "immune", "response", "."]} {"tags": [3, 4, 4, 0], "tokens": ["Glucocorticoid-regulated", "transcription", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoids", "are", "the", "most", "effective", "antiinflammatory", "drugs", "used", "in", "the", "treatment", "of", "asthma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["They", "act", "by", "binding", "to", "a", "specific", "receptor", "(", "GR", ")", "that", ",", "upon", "activation", ",", "translocates", "to", "the", "nucleus", "and", "either", "increases", "(", "transactivates", ")", "or", "decreases", "(", "transrepresses", ")", "gene", "expression", "."]} {"tags": [0, 0, 3, 4, 4, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "pro-inflammatory", "transcription", "factors", "such", "as", "activator", "protein", "(", "AP", ")", "-1", ",", "signal", "transducers", "and", "activators", "of", "transcription", "(", "STATs", ")", ",", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NFAT", ")", "and", "nuclear", "factor", "(", "NF", ")", "-kappa", "B", "is", "thought", "to", "be", "a", "major", "action", "of", "glucocorticoids", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 1, 2, 2, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0], "tokens": ["Acetylation", "of", "histones", "allows", "unwinding", "of", "the", "local", "DNA", "structure", "and", "enables", "RNA", "polymerase", "II", "to", "enhance", "gene", "transcription", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 3, 4, 0, 3, 0, 0], "tokens": ["Histone", "acetylation", "is", "regulated", "by", "a", "balance", "between", "the", "activity", "of", "histone", "acetyltransferases", "(", "HATs", ")", "and", "histone", "deacetylases", "(", "HDACs", ")", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0], "tokens": ["GR", "acts", "as", "a", "direct", "inhibitor", "of", "NF-kappa", "B-induced", "HAT", "activity", "and", "also", "by", "recruiting", "HDAC2", "to", "the", "NF-kappa", "B/HAT", "complex", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["A", "sub-group", "of", "patients", "with", "glucocorticoid-insensitive", "asthma", "have", "an", "inability", "to", "induce", "histone", "acetylation", "in", "response", "to", "dexamethasone", "suggesting", "reduced", "expression", "of", "a", "GR-specific", "HAT", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "suggests", "that", "pharmacological", "manipulation", "of", "specific", "histone", "acetylation", "status", "is", "a", "potentially", "useful", "approach", "for", "the", "treatment", "of", "inflammatory", "diseases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Identification", "of", "the", "precise", "mechanism", "by", "which", "activated", "GR", "recruits", "HDAC2", "may", "reveal", "new", "targets", "for", "the", "development", "of", "drugs", "that", "may", "dissociate", "the", "antiinflammatory", "actions", "of", "glucocorticoids", "from", "their", "side", "effects", "that", "are", "largely", "due", "to", "gene", "induction", "."]} {"tags": [0, 0, 0, 0], "tokens": ["Copyright", "Academic", "Press", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Differential", "ultraviolet-B-induced", "immunomodulation", "in", "XPA", ",", "XPC", ",", "and", "CSB", "DNA", "repair-deficient", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ultraviolet", "B", "irradiation", "has", "serious", "consequences", "for", "cellular", "immunity", "and", "can", "suppress", "the", "rejection", "of", "skin", "tumors", "and", "the", "resistance", "to", "infectious", "diseases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["DNA", "damage", "plays", "a", "crucial", "role", "in", "these", "immunomodulatory", "effects", "of", "ultraviolet", "B", ",", "as", "impaired", "repair", "of", "ultraviolet-B-induced", "DNA", "damage", "has", "been", "shown", "to", "cause", "suppression", "of", "cellular", "immunity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ultraviolet-B-induced", "DNA", "damage", "is", "repaired", "by", "the", "nucleotide", "excision", "repair", "mechanism", "very", "efficiently", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nucleotide", "excision", "repair", "comprises", "two", "subpathways", ":", "transcription-coupled", "and", "global", "genome", "repair", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", "the", "immunologic", "consequences", "of", "specific", "nucleotide", "excision", "repair", "defects", "in", "three", "mouse", "models", ",", "XPA", ",", "XPC", ",", "and", "CSB", "mutant", "mice", ",", "were", "investigated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["XPA", "mice", "carry", "a", "total", "nucleotide", "excision", "repair", "defect", ",", "whereas", "XPC", "and", "CSB", "mice", "only", "lack", "global", "genome", "and", "transcription-coupled", "nucleotide", "excision", "repair", ",", "respectively", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "data", "demonstrate", "that", "cellular", "immune", "parameters", "in", "XPA", ",", "XPC", ",", "and", "CSB", "mice", "are", "normal", "compared", "with", "their", "wild-type", "(", "control", ")", "littermates", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "may", "indicate", "that", "the", "reported", "altered", "cellular", "responses", "in", "xeroderma", "pigmentosum", "patients", "are", "not", "constitutive", "but", "could", "be", "due", "to", "external", "factors", ",", "such", "as", "ultraviolet", "B", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Upon", "exposure", "to", "ultraviolet", "B", ",", "only", "XPA", "mice", "are", "very", "sensitive", "to", "ultraviolet-B-induced", "inhibition", "of", "Th1-mediated", "contact", "hypersensitivity", "responses", "and", "interferon-gamma", "production", "in", "skin", "draining", "lymph", "nodes", "."]} {"tags": [0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lipopolysaccharide-stimulated", "tumor", "necrosis", "factor", "alpha", "and", "interleukin-10", "production", "are", "significantly", "augmented", "in", "both", "XPA", "and", "CSB", "mice", "after", "ultraviolet", "B", "exposure", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymph", "node", "cell", "numbers", "were", "increased", "very", "significantly", "in", "XPA", ",", "mildly", "increased", "in", "CSB", ",", "and", "not", "in", "XPC", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "general", "XPC", "mice", "do", "not", "exhibit", "any", "indication", "of", "enhanced", "ultraviolet", "B", "susceptibility", "with", "regard", "to", "the", "immune", "parameters", "analyzed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "suggest", "that", "both", "global", "genome", "repair", "and", "transcription-coupled", "repair", "are", "needed", "to", "prevent", "immunomodulation", "by", "ultraviolet", "B", ",", "whereas", "transcription-coupled", "repair", "is", "the", "major", "DNA", "repair", "subpathway", "of", "nucleotide", "excision", "repair", "that", "prevents", "the", "acute", "ultraviolet-B-induced", "effects", "such", "as", "erythema", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Biomechanical", "strain", "induces", "class", "a", "scavenger", "receptor", "expression", "in", "human", "monocyte/macrophages", "and", "THP-1", "cells", ":", "a", "potential", "mechanism", "of", "increased", "atherosclerosis", "in", "hypertension", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BACKGROUND", ":", "Although", "hypertension", "is", "an", "important", "risk", "factor", "for", "the", "development", "of", "atherosclerosis", ",", "the", "mechanisms", "for", "this", "interaction", "are", "incompletely", "described", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Previous", "studies", "have", "suggested", "that", "biomechanical", "strain", "regulates", "macrophage", "phenotype", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["We", "tested", "the", "hypothesis", "that", "biomechanical", "strain", "can", "induce", "expression", "of", "the", "class", "A", "scavenger", "receptor", "(", "SRA", ")", ",", "an", "important", "lipoprotein", "receptor", "in", "atherogenesis", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["METHODS", "AND", "RESULTS", ":", "Human", "monocyte/macrophages", "or", "THP-1", "cells", "were", "cultured", "in", "a", "device", "that", "imposes", "uniform", "biaxial", "cyclic", "1-Hz", "strains", "of", "0", "%", ",", "1", "%", ",", "2", "%", ",", "or", "3", "%", ",", "and", "SRA", "expression", "was", "analyzed", "."]} {"tags": [0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Mechanical", "strains", "induced", "SRA", "mRNA", "(", "3.5+/-0.6-fold", "at", "3", "%", "strain", "for", "48", "hours", ",", "P", "<", "0.01", ")", "and", "SRA", "protein", "in", "THP-1", "cells", "in", "an", "amplitude-dependent", "manner", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0], "tokens": ["This", "induction", "was", "accompanied", "by", "augmented", "expression", "of", "the", "class", "B", "scavenger", "receptor", "CD36", "(", "2.8+/-0.3-fold", ",", "P", "<", "0.001", ")", "but", "not", "by", "increased", "peroxisome", "proliferator-activated", "receptor-gamma", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "evaluate", "this", "effect", "in", "vivo", ",", "apolipoprotein", "E", "(", "-/-", ")", "mice", "were", "randomly", "assigned", "to", "receive", "standard", "chow", ",", "a", "high-cholesterol", "diet", ",", "or", "a", "high-cholesterol", "diet", "with", "hypertension", "induced", "by", "angiotensin", "II", "infusion", "for", "8", "weeks", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Immunohistochemistry", "revealed", "that", "among", "macrophages", "in", "atherosclerotic", "lesions", "of", "the", "aorta", ",", "the", "proportion", "of", "macrophages", "with", "SRA", "expression", "was", "highest", "in", "hypertensive", "animals", "on", "a", "high-cholesterol", "diet", "(", "43.9+/-0.7", "%", ",", "versus", "12.0+/-2.0", "%", "for", "normotensive", "animals", "on", "a", "high-cholesterol", "diet", "and", "4.7+/-4.7", "%", "for", "animals", "on", "standard", "chow", ";", "P", "<", "0.001", ")", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSIONS", ":", "Biomechanical", "strain", "induces", "SRA", "expression", "by", "monocyte/macrophages", ",", "suggesting", "a", "novel", "mechanism", "for", "promotion", "of", "atherosclerosis", "in", "hypertensive", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["High", "glucose", "induces", "MCP-1", "expression", "partly", "via", "tyrosine", "kinase-AP-1", "pathway", "in", "peritoneal", "mesothelial", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BACKGROUND", ":", "High", "glucose", "in", "peritoneal", "dialysis", "solutions", "has", "been", "implicated", "in", "the", "pathogenesis", "of", "peritoneal", "fibrosis", "in", "chronic", "ambulatory", "peritoneal", "dialysis", "(", "CAPD", ")", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "mechanisms", "are", "not", "very", "clear", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0], "tokens": ["Peritoneal", "macrophages", "seem", "to", "participate", "in", "the", "process", "of", "peritoneal", "fibrosis", "and", "monocyte", "chemoattractant", "protein-1", "(", "MCP-1", ")", "plays", "a", "key", "role", "in", "the", "recruitment", "of", "monocytes", "toward", "the", "peritoneal", "cavity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["However", ",", "little", "is", "known", "about", "the", "effect", "of", "high", "glucose", "on", "MCP-1", "expression", "and", "its", "signal", "transduction", "pathway", "in", "human", "peritoneal", "mesothelial", "cells", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Mesothelial", "cells", "were", "cultured", "with", "glucose", "(", "5", "to", "100", "mmol/L", ")", "or", "mannitol", "chronically", "for", "up", "to", "seven", "days", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["MCP-1", "expression", "of", "mRNA", "and", "protein", "was", "measured", "by", "Northern", "blot", "analysis", "and", "enzyme-linked", "immunosorbent", "assay", "(", "ELISA", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Chemotactic", "activity", "of", "high-glucose-conditioned", "culture", "supernatant", "was", "measured", "by", "chemotactic", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 3, 4, 0, 3, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "examine", "the", "roles", "of", "the", "transcription", "factors", "activator", "protein-1", "(", "AP-1", ")", "and", "nuclear", "factor-kappaB", "(", "NF-kappaB", ")", ",", "electrophoretic", "mobility", "shift", "assay", "(", "EMSA", ")", "was", "performed", "."]} {"tags": [0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "Glucose", "induced", "MCP-1", "mRNA", "expression", "in", "a", "time-", "and", "dose-dependent", "manner", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["MCP-1", "protein", "in", "cell", "culture", "supernant", "was", "also", "increased", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Equivalent", "concentrations", "of", "mannitol", "had", "no", "significant", "effect", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["High-glucose-conditioned", "supernatant", "possessed", "an", "increased", "chemotactic", "activity", "for", "monocytes", ",", "which", "was", "neutralized", "by", "anti-MCP-1", "antibody", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["EMSA", "revealed", "that", "glucose", "increased", "the", "AP-1", "binding", "activity", "in", "a", "time-", "and", "dose-dependent", "manner", ",", "but", "not", "NF-kappaB", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0], "tokens": ["Curcumin", ",", "an", "inhibitor", "of", "AP-1", ",", "dose-dependently", "suppressed", "the", "induction", "of", "MCP-1", "mRNA", "by", "high", "glucose", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Tyrosine", "kinase", "inhibitors", "such", "as", "genistein", "(", "12.5", "to", "50", "micromol/L", ")", "and", "herbimycin", "A", "(", "0.1", "to", "1", "micromol/L", ")", "inhibited", "the", "high-glucose-induced", "MCP-1", "mRNA", "expression", "in", "a", "dose-dependent", "manner", ",", "and", "also", "suppressed", "the", "high-glucose-induced", "AP-1", "binding", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["CONCLUSIONS", ":", ":", "High", "glucose", "induced", "mesothelial", "MCP-1", "expression", "partly", "via", "the", "tyrosine", "kinase-", "AP-1", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Signal", "thresholds", "and", "modular", "synergy", "during", "expression", "of", "costimulatory", "molecules", "in", "B", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 3, 0], "tokens": ["We", "analyzed", "intracellular", "pathways", "modulating", "surface", "densities", "of", "CD80", "and", "CD86", "in", "B", "cells", "activated", "through", "ligation", "of", "the", "Ag", "receptor", ",", "and", "the", "adhesion", "molecule", "CD54", "."]} {"tags": [0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Whereas", "B", "cell", "Ag", "receptor", "(", "BCR", ")", "cross-linking", "alone", "stimulated", "increased", "expression", "of", "CD86", ",", "up-regulation", "of", "CD80", "required", "dual", "stimulation", "with", "anti-IgM", "and", "anti-CD54", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "principal", "downstream", "component", "contributed", "by", "BCR", "signaling", ",", "toward", "both", "CD80", "and", "CD86", "induction", ",", "was", "the", "elevated", "concentration", "of", "free", "cytoplasmic", "Ca", "(", "2+", ")", ",", "recruited", "by", "way", "of", "capacitative", "influx", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["This", "alone", "was", "sufficient", "to", "generate", "an", "increase", "in", "CD86", "levels", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "CD80", "enhancement", "required", "the", "concerted", "action", "of", "both", "intracellular", "Ca", "(", "2+", ")", "concentration", "and", "CD54-initiated", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "nexus", "between", "anti-IgM", "and", "anti-CD54", "stimulation", ",", "in", "the", "context", "of", "CD80", "regulation", ",", "was", "identified", "to", "involve", "a", "self-propagating", "process", "of", "sequential", "synergy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "first", "step", "involved", "amplified", "accumulation", "of", "intracellular", "cAMP", ",", "as", "a", "result", "of", "cross-talk", "between", "BCR", "-mobilized", "Ca", "(", "2+", ")", "and", "CD54-derived", "signals", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["This", "then", "facilitated", "a", "second", "synergistic", "interaction", "between", "Ca", "(", "2+", ")", "and", "cAMP", ",", "culminating", "in", "CD80", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "findings", "of", "distinct", "signal", "transducer", "requirements", ",", "with", "the", "added", "consequences", "of", "cross-talk", ",", "offers", "an", "explanation", "for", "variable", "modulation", "of", "costimulatory", "molecule", "expression", "in", "response", "to", "diverse", "physiological", "stimuli", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Importantly", ",", "these", "results", "also", "reveal", "how", "concentration", "threshold", "barriers", "for", "recruitment", "of", "individual", "second", "messengers", "can", "be", "overcome", "by", "constructive", "convergence", "of", "signaling", "modules", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "role", "of", "Epstein-Barr", "virus", "in", "neoplastic", "transformation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "review", ",", "we", "focus", "on", "new", "data", "from", "basic", ",", "translational", "and", "clinical", "research", "relating", "to", "the", "Epstein-Barr", "virus", "(", "EBV", ")", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 0, 0, 0, 0, 5, 6, 0, 5, 0, 5, 0], "tokens": ["Beside", "its", "well-known", "tropism", "for", "B", "lymphocytes", "and", "epithelial", "cells", ",", "EBV", "also", "infects", "T", "lymphocytes", ",", "monocytes", "and", "granulocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["After", "primary", "infection", ",", "EBV", "persists", "throughout", "the", "life", "span", "in", "resting", "memory", "B", "cells", ",", "from", "where", "it", "is", "reactivated", "upon", "breakdown", "of", "cellular", "immunity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0], "tokens": ["In", "the", "process", "of", "neoplastic", "transformation", ",", "the", "EBV-encoded", "latent", "membrane", "protein", "1", "(", "LMP1", ")", "oncogene", "represents", "the", "major", "driving", "force", "."]} {"tags": [3, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0], "tokens": ["LMP1", "acts", "like", "a", "constitutively", "activated", "receptor", "of", "the", "tumor", "necrosis", "factor", "receptor", "family", "and", "allows", "the", "amplification", "or", "bypassing", "of", "physiological", "regulatory", "signals", "through", "direct", "and", "indirect", "interactions", "with", "proteins", "of", "the", "tumor", "necrosis", "factor", "receptor-associated", "factor", "(", "TRAF", ")", "family", "."]} {"tags": [3, 0, 3, 0, 0, 3, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0], "tokens": ["TRAF2", "-mediated", "NF-kappaB", "activation", ",", "AP-1", "induction", "and", "JAK3", "/", "STAT", "activation", "may", "result", "in", "sustained", "proliferation", "leading", "to", "lymphoma", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "ability", "of", "LMP1", "to", "suppress", "germinal", "center", "formation", "and", "its", "capacity", "to", "mediate", "its", "own", "transcriptional", "activation", "shed", "new", "light", "on", "the", "pathogenesis", "of", "EBV-associated", "latency", "type", "II", "lymphoproliferations", "like", "Hodgkin", "'s", "disease", "and", "angioimmunoblastic", "lymphadenopathy", "."]} {"tags": [0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "carboxy", "terminus", "of", "LMP1", "is", "also", "a", "reliable", "marker", "for", "individual", "EBV", "strain", "identification", "and", "thus", "offers", "new", "possibilities", "in", "tracing", "the", "molecular", "events", "leading", "to", "posttransplant", "lymphoproliferative", "disorders", "(", "PTLDs", ")", "."]} {"tags": [5, 6, 6, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0], "tokens": ["Cytotoxic", "T", "lymphocytes", "directed", "against", "well-characterized", "epitopes", "of", "EBV", "latency", "genes", "represent", "an", "already", "successful", "and", "promising", "therapeutic", "approach", "to", "EBV-associated", "lymphomas", ",", "in", "particular", "PTLDs"]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interferon-alpha", "drives", "T", "cell-mediated", "immunopathology", "in", "the", "intestine", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "ability", "of", "interferon", "(", "IFN", ")", "-alpha", "to", "induce", "autoimmunity", "and", "exacerbate", "Th1", "diseases", "is", "well", "known", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "recently", "described", "enhanced", "expression", "of", "IFN-alpha", "in", "the", "mucosa", "of", "patients", "with", "celiac", "disease", "(", "CD", ")", ",", "a", "gluten-sensitive", "Th1-mediated", "enteropathy", ",", "characterized", "by", "villous", "atrophy", "and", "crypt", "cell", "hyperplasia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Previous", "studies", "from", "this", "laboratory", "have", "shown", "that", "T", "cell", "activation", "in", "explant", "cultures", "of", "human", "fetal", "gut", "can", "also", "result", "in", "villous", "atrophy", "and", "crypt", "cell", "hyperplasia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 3, 0, 3, 0], "tokens": ["We", "have", ",", "therefore", ",", "examined", "changes", "that", "take", "place", "in", "explant", "cultures", "of", "human", "fetal", "gut", "after", "activation", "of", "T", "cells", "with", "anti-CD3", "and/or", "IFN-alpha", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["We", "show", "that", "activation", "of", "T", "cells", "with", "anti-CD3", "alone", "elicits", "a", "small", "IFN-gamma", "and", "TNF-alpha", "response", "with", "no", "tissue", "injury", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Similarly", ",", "no", "changes", "are", "seen", "in", "explants", "cultured", "with", "IFN-alpha", "alone", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "addition", "of", "IFN-alpha", "with", "anti-CD3", "results", "in", "enhanced", "Th1", "response", "and", "crypt", "cell", "hyperplasia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "is", "associated", "with", "enhanced", "phosphorylation", "of", "STAT1", ",", "STAT3", ",", "and", "Fyn", ",", "a", "Src", "homology", "tyrosine", "kinase", ",", "which", "interacts", "with", "both", "TCR", "and", "IFN-alpha", "signal", "components", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Together", "these", "data", "indicate", "that", "IFN-alpha", "can", "facilitate", "activation", "of", "Th1-reactive", "cells", "in", "the", "gut", "and", "drive", "immunopathology", "."]} {"tags": [0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Suppression", "of", "tumor", "necrosis", "factor", "alpha", "production", "by", "cAMP", "in", "human", "monocytes", ":", "dissociation", "with", "mRNA", "level", "and", "independent", "of", "interleukin-10", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 5, 6, 0], "tokens": ["BACKGROUND", ":", "Elevation", "of", "cellular", "cAMP", "inhibits", "lipopolysaccharide", "(", "LPS", ")", "-stimulated", "tumor", "necrosis", "factor", "alpha", "(", "TNF-alpha", ")", "production", "and", "increases", "the", "expression", "of", "interleukin", "(", "IL", ")", "-10", "in", "mononuclear", "cells", "."]} {"tags": [1, 2, 0, 0, 0, 0, 0, 3, 4, 3, 4, 4, 0, 3, 0, 0], "tokens": ["TNF-alpha", "gene", "expression", "obligates", "activation", "of", "the", "transcription", "factor", "nuclear", "factor", "kappaB", "(", "NF-kappaB", ")", "."]} {"tags": [0, 3, 0, 3, 0, 5, 0, 0, 0, 3, 0, 0], "tokens": ["Exogenous", "IL-10", "inhibits", "NF-kappaB", "in", "monocytes", "and", "thus", "attenuates", "TNF-alpha", "production", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 5, 6, 0, 0, 0], "tokens": ["We", "examined", "the", "role", "of", "endogenous", "IL-10", "in", "the", "regulation", "of", "NF-kappaB", "activation", "and", "TNF-alpha", "production", "in", "human", "monocytes", "by", "cAMP", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Human", "monocytes", "were", "stimulated", "with", "Escherichia", "coli", "LPS", "(", "100", "ng/ml", ")", "with", "and", "without", "forskolin", "(", "FSK", ",", "50", "microM", ")", "or", "dibutyryl", "cyclic", "AMP", "(", "dbcAMP", ",", "100", "microM", ")", "."]} {"tags": [3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cytokine", "(", "TNF-alpha", "and", "IL-10", ")", "release", "was", "measured", "by", "immunoassay", "."]} {"tags": [9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["TNF-alpha", "mRNA", "was", "measured", "by", "reverse", "transcription", "polymerase", "chain", "reaction", ",", "and", "NF-kappaB", "DNA", "binding", "activity", "was", "assessed", "by", "gel", "mobility", "shift", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "cAMP-elevating", "agents", "inhibited", "LPS-stimulated", "TNF-alpha", "release", "(", "0.77", "+/-", "0.13", "ng/10", "(", "6", ")", "cells", "in", "LPS", "+", "dbcAMP", "and", "0.68", "+/-", "0.19", "ng/10", "(", "6", ")", "cells", "in", "LPS", "+", "FSK", ",", "both", "P", "<", "0.05", "vs", "1.61", "+/-", "0.34", "ng/10", "(", "6", ")", "cells", "in", "LPS", "alone", ")", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Conversely", ",", "cAMP", "enhanced", "LPS-stimulated", "IL-10", "release", "(", "100", "+/-", "21.5", "pg/10", "(", "6", ")", "cells", "in", "LPS", "+", "dbcAMP", "and", "110", "+/-", "25.2", "pg/10", "(", "6", ")", "cells", "in", "LPS", "+", "FSK", ",", "both", "P", "<", "0.05", "vs", "53.3", "+/-", "12.8", "pg/10", "(", "6", ")", "cells", "in", "LPS", "alone", ")", "."]} {"tags": [0, 9, 10, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Neither", "TNF-alpha", "mRNA", "expression", "nor", "NF-kappaB", "activation", "stimulated", "by", "LPS", "was", "inhibited", "by", "the", "cAMP-elevating", "agents", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Neutralization", "of", "IL-10", "with", "a", "specific", "antibody", "did", "not", "attenuate", "the", "effect", "of", "cAMP-elevating", "agents", "on", "TNF-alpha", "production", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["CONCLUSION", ":", "The", "results", "indicate", "that", "cAMP", "inhibits", "LPS-stimulated", "TNF-alpha", "production", "through", "a", "posttranscriptional", "mechanism", "that", "is", "independent", "of", "endogenous", "IL-10", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Copyright", "2001", "Academic", "Press", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 2, 0, 3, 4, 4, 0, 0, 7, 8, 8, 0], "tokens": ["Activation", "of", "the", "p21", "(", "CIP1/WAF1", ")", "promoter", "by", "bone", "morphogenetic", "protein-2", "in", "mouse", "B", "lineage", "cells", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BMPs", "exert", "a", "negative", "growth", "effect", "on", "various", "types", "of", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "previously", "reported", "that", "BMP-2", "inhibited", "the", "growth", "of", "HS-72", "mouse", "hybridoma", "cells", "by", "inducing", "p21", "(", "CIP1/WAF1", ")", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 1, 2, 2, 2, 2, 0, 7, 8, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["In", "the", "present", "study", ",", "we", "demonstrated", "that", "BMP-2", "activated", "the", "mouse", "p21", "(", "CIP1/WAF1", ")", "promoter", "in", "HS-72", "cells", ",", "and", "that", "a", "29-base", "pair", "(", "b", ")", "region", "of", "the", "promoter", "(", "-1928/-1900", "relative", "to", "the", "TATA", "box", ")", ",", "conserved", "between", "mice", "and", "humans", ",", "was", "responsive", "to", "BMP-2", "as", "well", "as", "expression", "of", "Smad1", ",", "Smad4", ",", "and", "constitutively", "active", "mutants", "of", "BMP", "type", "I", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0, 7, 8, 0], "tokens": ["Furthermore", ",", "an", "oligonucleotide", "containing", "the", "29-b", "region", "was", "found", "to", "be", "associated", "with", "Smad4", "and", "phosphorylated", "Smad1", "in", "the", "nuclear", "extract", "of", "BMP-2", "-stimulated", "HS-72", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 1, 2, 0, 7, 8, 0], "tokens": ["These", "results", "suggested", "that", "BMP-2", "might", "activate", "p21", "(", "CIP1/WAF1", ")", "transcription", "by", "inducing", "a", "binding", "of", "Smad4", "and", "Smad1", "to", "the", "29-b", "region", "in", "HS-72", "cells", "."]} {"tags": [5, 6, 0, 0, 0, 5, 6, 0], "tokens": ["Dendritic", "cell", "development", "from", "common", "myeloid", "progenitors", "."]} {"tags": [5, 6, 0, 5, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Dendritic", "cells", "(", "DCs", ")", "are", "professional", "antigen-presenting", "cells", "which", "both", "initiate", "adaptive", "immune", "responses", "and", "control", "tolerance", "to", "self-antigens", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "has", "been", "suggested", "that", "these", "different", "effects", "on", "responder", "cells", "depend", "on", "subsets", "of", "DCs", "arising", "from", "either", "myeloid", "or", "lymphoid", "hematopoietic", "origins", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "model", ",", "CD8", "alpha+", "Mac-1-", "DCs", "are", "supposed", "to", "be", "of", "lymphoid", "while", "CD8", "alpha-", "Mac-1+", "DCs", "are", "supposed", "to", "be", "of", "myeloid", "origin", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 5, 6, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "summarize", "our", "findings", "that", "both", "CD8", "alpha+", "and", "CD8", "alpha-", "DCs", "can", "arise", "from", "clonogenic", "common", "myeloid", "progenitors", "(", "CMPs", ")", "in", "both", "thymus", "and", "spleen", "."]} {"tags": [0, 3, 4, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", "CD8", "alpha", "expression", "DCs", "does", "not", "indicate", "a", "lymphoid", "origin", "and", "differences", "among", "CD8", "alpha+", "and", "CD8", "alpha-", "DCs", "might", "rather", "reflect", "maturation", "status", "than", "ontogeny", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["On", "the", "basis", "of", "transplantation", "studies", ",", "it", "seems", "likely", "that", "most", "of", "the", "DCs", "in", "secondary", "lymphoid", "organs", "and", "a", "substantial", "fraction", "of", "thymic", "DCs", "are", "myeloid-derived", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 3, 0, 0, 0, 3, 0, 3, 4, 4, 4, 4, 0], "tokens": ["Macrophage", "stimulation", "with", "Murabutide", ",", "an", "HIV-suppressive", "muramyl", "peptide", "derivative", ",", "selectively", "activates", "extracellular", "signal-regulated", "kinases", "1", "and", "2", ",", "C/EBPbeta", "and", "STAT1", ":", "role", "of", "CD14", "and", "Toll-like", "receptors", "2", "and", "4", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "smallest", "unit", "of", "bacterial", "peptidoglycans", "known", "to", "be", "endowed", "with", "biological", "activities", "is", "muramyl", "dipeptide", "(", "MDP", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0], "tokens": ["A", "clinically", "acceptable", "synthetic", "derivative", "of", "MDP", ",", "namely", "murabutide", "(", "MB", ")", ",", "has", "been", "found", "to", "present", "interesting", "pharmacological", "properties", "and", "to", "suppress", "HIV-1", "replication", "in", "monocyte-derived", "macrophages", "(", "MDM", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "addressed", "the", "signaling", "events", "activated", "in", "MDM", "following", "stimulation", "with", "either", "MB", "or", "the", "potent", "immunostimulant", "LPS", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 0, 3, 4, 4, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "also", "examined", "whether", "signaling", "by", "muramyl", "peptides", "involves", "the", "use", "of", "cell", "surface", "receptors", ",", "including", "CD14", "and", "Toll-like", "receptor", "2", "(", "TLR2", ")", "or", "TLR4", "that", "are", "known", "to", "be", "signal-transducing", "receptors", "for", "other", "bacterial", "cell", "wall", "components", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 4, 0, 0], "tokens": ["We", "demonstrate", "that", ",", "unlike", "LPS", ",", "the", "safe", "immunomodulator", "MB", "selectively", "activates", "extracellular", "signal-regulated", "kinases", "(", "Erk", ")", "1/2", ",", "in", "the", "absence", "of", "detectable", "Jun", "N-terminal", "kinase", "(", "JNK", ")", "or", "p38", "mitogen-activated", "kinase", "activation", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Furthermore", ",", "STAT1", "activation", "but", "weak", "or", "no", "activation", "of", "STAT3", "or", "STAT5", "respectively", ",", "could", "be", "detected", "in", "MB-stimulated", "MDM", "."]} {"tags": [0, 7, 8, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Using", "MonoMac6", "cells", ",", "we", "observed", "high", "C/EBPbeta", "and", "AP-1", "but", "weaker", "and", "transient", "NF-kappaB", "activation", "by", "MB", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Moreover", ",", "the", "truncated", "form", "of", "C/EBPbeta", ",", "known", "to", "repress", "HIV-1", "transcription", ",", "was", "detected", "in", "extracts", "from", "MB-treated", "THP-1", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 0], "tokens": ["Surprisingly", ",", "neither", "MB", "nor", "MDP", "were", "able", "to", "transduce", "signals", "via", "CD14", "and", "TLR2", "or", "4", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "findings", "present", "major", "differences", "in", "the", "early", "cell", "activation", "process", "between", "LPS", "and", "muramyl", "peptides", ",", "and", "strongly", "argue", "for", "the", "implication", "of", "co-receptors", "other", "than", "TLR2", "and", "TLR4", "in", "mediating", "the", "signaling", "events", "induced", "by", "defined", "subunits", "of", "bacterial", "peptidoglycans", "."]} {"tags": [3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nuclear", "peroxisome", "proliferator-activated", "receptors", "alpha", "and", "gamma", "have", "opposing", "effects", "on", "monocyte", "chemotaxis", "in", "endometriosis", "."]} {"tags": [0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "peroxisome", "proliferator-activated", "receptors", "(", "PPARs", ")", "alpha", "and", "gamma", "are", "nuclear", "receptors", "that", "play", "important", "roles", "in", "inflammatory", "diseases", "like", "ulcerative", "colitis", "and", "arthritis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", ",", "we", "examined", "the", "possible", "role", "of", "PPARs", "in", "macrophage", "attraction", "into", "the", "peritoneal", "cavity", "of", "patients", "with", "endometriosis", "."]} {"tags": [0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 7, 8, 8, 0], "tokens": ["We", "identified", "PPAR-alpha", "and", "-gamma", "messenger", "RNA", "by", "RT-PCR", "and", "protein", "by", "immunoblotting", "of", "lysates", "of", "peritoneal", "macrophages", "and", "monocytic", "U937", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "immunocytochemistry", ",", "we", "localized", "PPAR-alpha", "and", "-gamma", "within", "the", "nuclei", "of", "both", "cell", "types", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Monocyte", "chemotactic", "activity", "of", "peritoneal", "fluid", "from", "patients", "with", "endometriosis", "was", "quantified", "in", "Boyden", "chambers", "."]} {"tags": [0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Migration", "of", "U937", "cells", "was", "increased", "by", "WY", "14643", "and", "reduced", "by", "rosiglitazone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Peritoneal", "fluid", "from", "patients", "with", "endometriosis", "activated", "U937", "cells", "transiently", "transfected", "with", "a", "PPAR-alpha/GAL4", "luciferase", "reporter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["By", "contrast", ",", "peritoneal", "fluid", "did", "not", "cause", "significant", "activation", "of", "PPAR-gamma/GAL4", "constructs", "."]} {"tags": [0, 7, 8, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "U937", "cells", "transiently", "transfected", "with", "a", "PPAR", "response", "element", "luciferase", "reporter", "showed", "disease", "stage-dependent", "up-regulation", "when", "treated", "with", "peritoneal", "fluid", "from", "patients", "with", "endometriosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Treatment", "with", "peritoneal", "fluid", "from", "healthy", "controls", "down-regulated", "PPAR", "response", "element", "transactivation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["We", "conclude", "that", "peritoneal", "fluid", "of", "endometriosis", "patients", "contains", "activators", "of", "PPAR-alpha", "that", "stimulate", "macrophage", "chemotaxis", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibitors", "of", "PPAR-alpha", "or", "activators", "of", "PPAR-gamma", "could", "be", "developed", "for", "the", "treatment", "of", "inflammation", "associated", "with", "endometriosis", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 4, 0, 5, 6, 6, 6, 0, 0, 0, 0], "tokens": ["Long-term-impaired", "expression", "of", "nuclear", "factor-kappa", "B", "and", "I", "kappa", "B", "alpha", "in", "peripheral", "blood", "mononuclear", "cells", "of", "trauma", "patients", "."]} {"tags": [3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nuclear", "factor", "(", "NF", ")", "-kappa", "B", "expression", "and", "dimer", "characteristics", "were", "studied", "in", "peripheral", "blood", "mononuclear", "cells", "(", "PBMCs", ")", "of", "major-trauma", "patients", "and", "healthy", "controls", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Analysis", "of", "PBMCs", "on", "days", "1", ",", "3", ",", "5", ",", "and", "10", "after", "trauma", "revealed", "that", "expression", "of", "both", "p65p50", "heterodimers", "and", "p50p50", "homodimers", "was", "significantly", "reduced", "compared", "with", "that", "in", "controls", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0], "tokens": ["In", "vitro", "lipopolysaccharide", "(", "LPS", ")", "stimulation", "of", "PBMCs", "induced", "NF-kappa", "B", "translocation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "throughout", "the", "survey", ",", "p65p50", "activation", "remained", "significantly", "lower", "in", "trauma", "patients", "than", "in", "controls", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["After", "LPS", "stimulation", "in", "vitro", ",", "the", "p65p50/p50p50", "ratio", "was", "significantly", "lower", "in", "PBMCs", "from", "trauma", "patients", "than", "from", "healthy", "controls", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "ex", "vivo", "expression", "of", "I", "kappa", "B", "alpha", "was", "higher", "in", "PBMCs", "of", "controls", "than", "of", "trauma", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["LPS", "did", "not", "induce", "I", "kappa", "B", "expression", "in", "PBMCs", "from", "trauma", "patients", ",", "but", "strong", "induction", "was", "obtained", "with", "staphylococci", ",", "suggesting", "that", "this", "defect", "is", "not", "universal", "and", "depends", "on", "the", "nature", "of", "the", "activating", "signal", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "no", "direct", "correlation", "was", "found", "between", "levels", "of", "interleukin-10", "or", "transforming", "growth", "factor-beta", "and", "NF-kappa", "B", ",", "these", "immunosuppressive", "cytokines", "were", "significantly", "elevated", "in", "trauma", "patients", "by", "10", "days", "after", "admission", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "long-term", "low-basal", "and", "LPS-induced", "nuclear", "translocation", "of", "NF-kappa", "B", "recalled", "long-term", "immunoparalysis", "observed", "in", "patients", "with", "severe", "inflammatory", "stress", "such", "as", "trauma", "."]} {"tags": [0, 0, 0, 0], "tokens": ["Hepatic", "ischemia-reperfusion", "injury", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BACKGROUND", ":", "The", "morbidity", "associated", "with", "liver", "transplantation", "and", "major", "hepatic", "resections", "is", "partly", "a", "result", "of", "ischemia-reperfusion", "injury", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["DATA", "SOURCES", ":", "The", "entire", "world", "literature", "on", "the", "subject", "was", "searched", "via", "Medline", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Keywords", "included", "reperfusion", "injury", ",", "transplantation", ",", "liver", "resection", ",", "nitric", "oxide", ",", "endothelin", ",", "cytokines", ",", "Kupffer", "cells", ",", "ischemic/ischaemic", "preconditioning", ",", "and", "nuclear", "factor-kappa", "B", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSIONS", ":", "An", "imbalance", "between", "endothelin", "and", "nitric", "oxide", "levels", "results", "in", "failure", "of", "the", "hepatic", "microcirculation", "at", "the", "onset", "of", "reperfusion", "."]} {"tags": [0, 0, 3, 4, 4, 0, 0, 0, 0, 3, 4, 0, 3, 4, 0, 0], "tokens": ["Activation", "of", "nuclear", "factor-kappa", "B", "in", "the", "liver", "promotes", "proinflammatory", "cytokine", "and", "adhesion", "molecule", "synthesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "result", "in", "oxygen-derived", "free", "radical", "production", "and", "neutrophil", "recruitment", ",", "further", "contributing", "to", "cellular", "injury", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Various", "therapeutic", "modalities", "acting", "on", "the", "above", "mediators", "have", "been", "successfully", "used", "to", "attenuate", "reperfusion", "injury", "in", "animal", "models", "of", "hepatic", "transplantation", "and", "resection", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Application", "of", "the", "knowledge", "gained", "from", "animal", "models", "of", "hepatic", "ischemia-reperfusion", "to", "the", "clinical", "setting", "will", "improve", "the", "outcome", "of", "hepatic", "surgery", "."]} {"tags": [0, 0, 1, 2, 2, 0, 0, 0, 0, 0], "tokens": ["Down-regulation", "of", "IL-12", "p40", "gene", "in", "Plasmodium", "berghei-infected", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0], "tokens": ["We", "analyzed", "the", "mechanism", "that", "causes", "suppression", "of", "IL-12", "p40", "gene", "induction", "during", "Plasmodium", "berghei", "infection", "."]} {"tags": [0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Although", "IL-12", "together", "with", "IFN-gamma", "plays", "an", "important", "role", "in", "protection", "against", "pathogenic", "infection", ",", "the", "IL-12", "p70", "protein", "production", "of", "infected", "macrophages", "is", "lower", "than", "that", "by", "the", "uninfected", "macrophages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 2, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "showed", "in", "the", "present", "study", "that", "the", "induction", "of", "IL-12", "p40", "gene", "but", "not", "IL-12", "p35", "gene", "in", "macrophages", "of", "P.", "berghei-infected", "mice", "was", "profoundly", "inhibited", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["The", "inhibition", "was", "induced", "by", "interaction", "with", "macrophages", "that", "had", "contacted", "with", "P.", "berghei-infected", "erythrocytes", "and", "was", "mediated", "by", "a", "soluble", "factor", ",", "IL-10", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 6, 0], "tokens": ["There", "was", "comparable", "activation", "of", "NF-kappaB", "in", "uninfected", "and", "infected", "cells", "."]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 5, 6, 0], "tokens": ["The", "induction", "of", "IFN-regulatory", "factor-1", "gene", "was", "comparable", "in", "transcription", "level", "in", "uninfected", "and", "infected", "cells", ",", "while", "the", "unidentified", "complex", "formation", "of", "IFN-regulatory", "factor-1", "was", "observed", "in", "infected", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", ",", "the", "inhibition", "of", "the", "IL-12", "p40", "gene", "induction", "appeared", "to", "be", "regulated", "at", "transcriptional", "regulation", "level", "of", "the", "gene", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "Friend", "of", "GATA", "proteins", "U-shaped", ",", "FOG-1", ",", "and", "FOG-2", "function", "as", "negative", "regulators", "of", "blood", ",", "heart", ",", "and", "eye", "development", "in", "Drosophila", "."]} {"tags": [3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Friend", "of", "GATA", "(", "FOG", ")", "proteins", "regulate", "GATA", "factor-activated", "gene", "transcription", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["During", "vertebrate", "hematopoiesis", ",", "FOG", "and", "GATA", "proteins", "cooperate", "to", "promote", "erythrocyte", "and", "megakaryocyte", "differentiation", "."]} {"tags": [0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "Drosophila", "FOG", "homologue", "U-shaped", "(", "Ush", ")", "is", "expressed", "similarly", "in", "the", "blood", "cell", "anlage", "during", "embryogenesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0, 0], "tokens": ["During", "hematopoiesis", ",", "the", "acute", "myeloid", "leukemia", "1", "homologue", "Lozenge", "and", "Glial", "cells", "missing", "are", "required", "for", "the", "production", "of", "crystal", "cells", "and", "plasmatocytes", ",", "respectively", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "additional", "factors", "have", "been", "predicted", "to", "control", "crystal", "cell", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 3, 0, 0, 0], "tokens": ["In", "this", "report", ",", "we", "show", "that", "Ush", "is", "expressed", "in", "hemocyte", "precursors", "and", "plasmatocytes", "throughout", "embryogenesis", "and", "larval", "development", ",", "and", "the", "GATA", "factor", "Serpent", "is", "essential", "for", "Ush", "embryonic", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "loss", "of", "ush", "function", "results", "in", "an", "overproduction", "of", "crystal", "cells", ",", "whereas", "forced", "expression", "of", "Ush", "reduces", "this", "cell", "population", "."]} {"tags": [3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Murine", "FOG-1", "and", "FOG-2", "also", "can", "repress", "crystal", "cell", "production", ",", "but", "a", "mutant", "version", "of", "FOG-2", "lacking", "a", "conserved", "motif", "that", "binds", "the", "corepressor", "C-terminal", "binding", "protein", "fails", "to", "affect", "the", "cell", "lineage", "."]} {"tags": [0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "GATA", "factor", "Pannier", "(", "Pnr", ")", "is", "required", "for", "eye", "and", "heart", "development", "in", "Drosophila", "."]} {"tags": [0, 3, 0, 3, 0, 3, 0, 0, 3, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["When", "Ush", ",", "FOG-1", ",", "FOG-2", ",", "or", "mutant", "FOG-2", "is", "coexpressed", "with", "Pnr", "during", "these", "developmental", "processes", ",", "severe", "eye", "and", "heart", "phenotypes", "result", ",", "consistent", "with", "a", "conserved", "negative", "regulation", "of", "Pnr", "function", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8], "tokens": ["These", "results", "indicate", "that", "the", "fly", "and", "mouse", "FOG", "proteins", "function", "similarly", "in", "three", "distinct", "cellular", "contexts", "in", "Drosophila", ",", "but", "may", "use", "different", "mechanisms", "to", "regulate", "genetic", "events", "in", "blood", "vs", ".", "cardial", "or", "eye", "cell", "lineages"]} {"tags": [0, 3, 4, 4, 3, 0, 0, 0, 0, 0, 3, 4, 3, 0], "tokens": ["The", "RING", "finger", "protein", "Siah-1", "regulates", "the", "level", "of", "the", "transcriptional", "coactivator", "OBF-1", "."]} {"tags": [0, 3, 4, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "transcriptional", "coactivator", "OBF-1", ",", "which", "interacts", "with", "Oct-1", "and", "Oct-2", "and", "the", "octamer", "site", "DNA", ",", "has", "been", "shown", "to", "be", "critical", "for", "development", "of", "a", "normal", "immune", "response", "and", "the", "formation", "of", "germinal", "centers", "in", "secondary", "lymphoid", "organs", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 3, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Here", "we", "have", "identified", "the", "RING", "finger", "protein", "Siah-1", "as", "a", "protein", "interacting", "specifically", "with", "OBF-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["This", "interaction", "is", "mediated", "by", "the", "C-terminal", "part", "of", "Siah-1", "and", "by", "residues", "in", "the", "N-terminus", "of", "OBF-1", ",", "partly", "distinct", "from", "the", "residues", "required", "for", "formation", "of", "a", "complex", "with", "the", "Oct", "POU", "domains", "and", "the", "DNA", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interaction", "between", "Siah-1", "and", "OBF-1", "leads", "to", "downregulation", "of", "OBF-1", "protein", "level", "but", "not", "mRNA", ",", "and", "to", "a", "corresponding", "reduction", "in", "octamer", "site-dependent", "transcription", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Inhibition", "of", "the", "ubiquitin-proteasome", "pathway", "in", "B", "cells", "leads", "to", "elevated", "levels", "of", "OBF-1", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 9, 10, 0], "tokens": ["Furthermore", ",", "in", "immunized", "mice", ",", "OBF-1", "protein", "amounts", "are", "dramatically", "increased", "in", "primary", "activated", "B", "cells", ",", "without", "concomitant", "increase", "in", "OBF-1", "mRNA", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0], "tokens": ["These", "data", "suggest", "that", "Siah-1", "is", "part", "of", "a", "novel", "regulatory", "loop", "controlling", "the", "level", "of", "OBF-1", "protein", "in", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0], "tokens": ["The", "beta-catenin", "--", "TCF-1", "pathway", "ensures", "CD4", "(", "+", ")", "CD8", "(", "+", ")", "thymocyte", "survival", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "association", "of", "trans-acting", "T", "cell", "factors", "(", "TCFs", ")", "or", "lymphoid", "enhancer", "factor", "1", "(", "LEF-1", ")", "with", "their", "coactivator", "beta-catenin", "mediates", "transient", "transcriptional", "responses", "to", "extracellular", "Wnt", "signals", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0], "tokens": ["We", "show", "here", "that", "T", "cell", "maturation", "depends", "on", "the", "presence", "of", "the", "beta-catenin", "--", "binding", "domain", "in", "TCF-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0], "tokens": ["This", "domain", "is", "necessary", "to", "mediate", "the", "survival", "of", "immature", "CD4", "(", "+", ")", "CD8", "(", "+", ")", "double-positive", "(", "DP", ")", "thymocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 4, 4, 4, 0], "tokens": ["Accelerated", "spontaneous", "thymocyte", "death", "in", "the", "absence", "of", "TCF-1", "correlates", "with", "aberrantly", "low", "expression", "of", "the", "anti-apoptotic", "protein", "Bcl-x", "(", "L", ")", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 6, 0, 0, 0], "tokens": ["Increasing", "anti-apoptotic", "effectors", "in", "thymocytes", "by", "the", "use", "of", "a", "Bcl-2", "transgene", "rescued", "TCF-1-deficient", "DP", "thymocytes", "from", "apoptosis", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Thus", ",", "TCF-1", ",", "upon", "association", "with", "beta-catenin", ",", "transiently", "ensures", "the", "survival", "of", "immature", "T", "cells", ",", "which", "enables", "them", "to", "generate", "and", "edit", "T", "cell", "receptor", "(", "TCR", ")", "alpha", "chains", "and", "attempt", "TCR", "-mediated", "positive", "selection", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["TRAIL/Apo2L", "ligand", "selectively", "induces", "apoptosis", "and", "overcomes", "drug", "resistance", "in", "multiple", "myeloma", ":", "therapeutic", "applications", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Multiple", "myeloma", "(", "MM", ")", "remains", "incurable", "and", "novel", "treatments", "are", "urgently", "needed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0], "tokens": ["Preclinical", "in", "vitro", "and", "in", "vivo", "evaluations", "were", "performed", "to", "assess", "the", "potential", "therapeutic", "applications", "of", "human", "recombinant", "tumor", "necrosis", "factor", "(", "TNF", ")", "-related", "apoptosis-inducing", "ligand/Apo2", "ligand", "(", "TRAIL/Apo2L", ")", "in", "MM", "."]} {"tags": [3, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["TRAIL/Apo2L", "potently", "induced", "apoptosis", "of", "MM", "cells", "from", "patients", "and", "the", "majority", "of", "MM", "cell", "lines", ",", "including", "cells", "sensitive", "or", "resistant", "to", "dexamethasone", "(", "Dex", ")", ",", "doxorubicin", "(", "Dox", ")", ",", "melphalan", ",", "and", "mitoxantrone", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["TRAIL/Apo2L", "also", "overcame", "the", "survival", "effect", "of", "interleukin", "6", "on", "MM", "cells", "and", "did", "not", "affect", "the", "survival", "of", "peripheral", "blood", "and", "bone", "marrow", "mononuclear", "cells", "and", "purified", "B", "cells", "from", "healthy", "donors", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "status", "of", "the", "TRAIL", "receptors", "(", "assessed", "by", "immunoblotting", "and", "flow", "cytometry", ")", "could", "not", "predict", "TRAIL", "sensitivity", "of", "MM", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["The", "anti-MM", "activity", "of", "TRAIL/Apo2L", "was", "confirmed", "in", "nu/xid/bg", "mice", "xenografted", "with", "human", "MM", "cells", ";", "TRAIL", "(", "500", "microg", "intraperitoneally", "daily", "for", "14", "days", ")", "was", "well", "tolerated", "and", "significantly", "suppressed", "the", "growth", "of", "plasmacytomas", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Dox", "up-regulated", "the", "expression", "of", "the", "TRAIL", "receptor", "death", "receptor", "5", "(", "DR5", ")", "and", "synergistically", "enhanced", "the", "effect", "of", "TRAIL", "not", "only", "against", "MM", "cells", "sensitive", "to", ",", "but", "also", "against", "those", "resistant", "to", ",", "Dex-", "or", "Dox-induced", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["Nuclear", "factor", "(", "NF", ")", "-kappaB", "inhibitors", ",", "such", "as", "SN50", "(", "a", "cell-permeable", "inhibitor", "of", "the", "nuclear", "translocation", "and", "transcriptional", "activity", "of", "NF-kappaB", ")", "or", "the", "proteasome", "inhibitor", "PS-341", ",", "enhanced", "the", "proapoptotic", "activity", "of", "TRAIL/Apo2L", "against", "TRAIL-sensitive", "MM", "cells", ",", "whereas", "SN50", "reversed", "the", "TRAIL", "resistance", "of", "ARH-77", "and", "IM-9", "MM", "cells", "."]} {"tags": [0, 0, 5, 6, 6, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Importantly", ",", "normal", "B", "lymphocytes", "were", "not", "sensitized", "to", "TRAIL", "by", "either", "Dox", ",", "SN50", ",", "or", "PS-341", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "preclinical", "studies", "suggest", "that", "TRAIL/Apo2L", "can", "overcome", "conventional", "drug", "resistance", "and", "provide", "the", "basis", "for", "clinical", "trials", "of", "TRAIL", "-based", "treatment", "regimens", "to", "improve", "outcome", "in", "patients", "with", "MM", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["(", "Blood.", "2001", ";", "98", ":", "795-804", ")"]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 0, 0, 3, 0, 0, 5, 6, 6, 0], "tokens": ["CD28", "costimulation", "is", "required", "not", "only", "to", "induce", "IL-12", "receptor", "but", "also", "to", "render", "janus", "kinases/STAT4", "responsive", "to", "IL-12", "stimulation", "in", "TCR-triggered", "T", "cells", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "activation", "of", "resting", "T", "cells", "for", "the", "acquisition", "of", "various", "functions", "depends", "on", "whether", "CD28", "costimulatory", "signals", "are", "provided", "upon", "T", "cell", "receptor", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0, 0, 3, 0, 0], "tokens": ["Here", ",", "we", "investigated", "how", "CD28", "costimulation", "functions", "to", "allow", "TCR", "-triggered", "resting", "T", "cells", "to", "acquire", "IL-12", "responsiveness", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0], "tokens": ["When", "T", "cells", "are", "stimulated", "with", "low", "doses", "of", "anti-CD3", "mAb", ",", "CD28", "costimulation", "was", "required", "for", "the", "optimal", "levels", "of", "IL-12", "receptor", "(", "IL-12R", ")", "expression", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "stimulation", "of", "T", "cells", "with", "high", "doses", "of", "anti-CD3", "alone", "induced", "comparable", "levels", "of", "IL-12R", "expression", "to", "those", "induced", "upon", "CD28", "costimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 5, 6, 6, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Nevertheless", ",", "there", "was", "a", "substantial", "difference", "in", "IL-12", "responsiveness", "between", "these", "two", "groups", "of", "T", "cells", ":", "compared", "to", "anti-CD28-costimulated", "T", "cells", ",", "T", "cells", "that", "were", "not", "costimulated", "with", "anti-CD28", "exhibited", "decreased", "levels", "of", "Janus", "kinases", "(", "JAK", ")", "JAK2/TYK2", "and", "STAT4", "phosphorylation", "and", "IFN-y", "production", "following", "IL-12", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Importantly", ",", "STAT6", "phosphorylation", "following", "IL-4", "stimulation", "was", "not", "decreased", "in", "anti-CD28-uncostimulated", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0], "tokens": ["These", "resutls", "indicate", "that", "CD28", "costimulation", "not", "only", "contributes", "to", "up-regulating", "IL-12R", "expression", "but", "is", "also", "required", "to", "render", "JAKs/STAT4", "responsive", "to", "IL-12", "stimulation", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Constitutively", "activated", "Akt-1", "is", "vital", "for", "the", "survival", "of", "human", "monocyte-differentiated", "macrophages", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Role", "of", "Mcl-1", ",", "independent", "of", "nuclear", "factor", "(", "NF", ")", "-kappaB", ",", "Bad", ",", "or", "caspase", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 1, 2, 0, 0, 1, 2, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 0, 0], "tokens": ["Recent", "data", "from", "mice", "deficient", "for", "phosphatase", "and", "tensin", "homologue", "deleted", "from", "chromosome", "10", "or", "src", "homology", "2", "domain-containing", "5", "'", "inositol", "phosphatase", ",", "phosphatases", "that", "negatively", "regulate", "the", "phosphatidylinositol", "3-kinase", "(", "PI3K", ")", "pathway", ",", "revealed", "an", "increased", "number", "of", "macrophages", "in", "these", "animals", ",", "suggesting", "an", "essential", "role", "for", "the", "PI3K", "pathway", "for", "macro-phage", "survival", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 3, 0, 0, 5, 0, 0], "tokens": ["Here", ",", "we", "focused", "on", "the", "role", "of", "the", "PI3K-regulated", "serine/threonine", "kinase", "Akt-1", "in", "modulating", "macrophage", "survival", "."]} {"tags": [3, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Akt-1", "was", "constitutively", "activated", "in", "human", "macrophages", "and", "addition", "of", "the", "PI3K", "inhibitor", ",", "LY294002", ",", "suppressed", "the", "activation", "of", "Akt-1", "and", "induced", "cell", "death", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "suppression", "of", "Akt-1", "by", "inhibition", "of", "PI3K", "or", "a", "dominant", "negative", "(", "DN", ")", "Akt-1", "resulted", "in", "loss", "of", "mitochondrial", "transmembrane", "potential", ",", "activation", "of", "caspases-9", "and", "-3", ",", "and", "DNA", "fragmentation", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 4, 0], "tokens": ["The", "effects", "of", "PI3K", "inhibition", "were", "reversed", "by", "the", "ectopic", "expression", "of", "constitutively", "activated", "Akt-1", "or", "Bcl-x", "(", "L", ")", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 5, 6, 0], "tokens": ["Inhibition", "of", "PI3K", "/Akt-1", "pathway", "either", "by", "LY294002", "or", "DN", "Akt-1", "had", "no", "effect", "on", "the", "constitutive", "or", "inducible", "activation", "of", "nuclear", "factor", "(", "NF", ")", "-kappaB", "in", "human", "macrophages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 3, 0, 5, 0, 0, 0, 0, 0], "tokens": ["However", ",", "after", "inhibition", "of", "the", "PI3K", "/", "Akt-1", "pathway", ",", "a", "marked", "decrease", "in", "the", "expression", "of", "the", "antiapoptotic", "molecule", "Mcl-1", ",", "but", "not", "other", "Bcl-2", "family", "members", "was", "observed", ",", "and", "Mcl-1", "rescued", "macrophages", "from", "LY294002-induced", "cell", "death", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["Further", ",", "inhibition", "of", "Mcl-1", "by", "antisense", "oligonucleotides", ",", "also", "resulted", "in", "macrophage", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0], "tokens": ["Thus", ",", "our", "findings", "demonstrate", "that", "the", "constitutive", "activation", "of", "Akt-1", "regulates", "macrophage", "survival", "through", "Mcl-1", ",", "which", "is", "independent", "of", "caspases", ",", "NF-kappaB", ",", "or", "Bad", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["GRbeta", "expression", "in", "nasal", "polyp", "inflammatory", "cells", "and", "its", "relationship", "to", "the", "anti-inflammatory", "effects", "of", "intranasal", "fluticasone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BACKGROUND", ":", "Nasal", "polyposis", "disease", "is", "an", "inflammatory", "disorder", "with", "intense", "eosinophilic", "infiltration", "of", "respiratory", "mucosa", "that", "is", "often", "difficult", "to", "control", "with", "topical", "steroids", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Recent", "evidence", "suggests", "that", "overexpression", "of", "the", "glucocorticoid", "receptor", "splice", "variant", "GRbeta", "in", "inflammatory", "cells", "might", "contribute", "to", "steroid", "insensitivity", "in", "diseases", "such", "as", "asthma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["OBJECTIVE", ":", "The", "purposes", "of", "this", "investigation", "were", "to", "determine", "whether", "nasal", "polyp", "(", "NP", ")", "inflammatory", "cells", "overexpress", "GRbeta", "and", "to", "examine", "whether", "GRbeta", "overexpression", "is", "associated", "with", "insensitivity", "to", "the", "potent", "topical", "steroid", "fluticasone", "propionate", "(", "FP", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Biopsies", "were", "obtained", "from", "10", "subjects", "with", "NPs", "before", "and", "4", "weeks", "after", "treatment", "with", "intranasal", "FP", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Middle", "turbinates", "biopsies", "from", "6", "healthy", ",", "nonallergic", "subjects", "served", "as", "normal", "controls", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 9, 10, 0], "tokens": ["Biopsies", "were", "immunostained", "for", "inflammatory", "cell", "markers", "as", "well", "as", "GRbeta", "and", "probed", "for", "various", "cytokine", "mRNA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "anti-inflammatory", "response", "to", "FP", "was", "examined", "in", "relation", "to", "pretreatment", "levels", "of", "GRbeta", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 5, 0], "tokens": ["RESULTS", ":", "The", "total", "numbers", "of", "inflammatory", "cells", "were", "increased", "in", "NPs", "."]} {"tags": [0, 0, 0, 5, 6, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "percentage", "of", "inflammatory", "cells", "expressing", "GRbeta", "was", "also", "increased", "(", "40.5", "%", "+/-", "19.2", "%", "vs", "16.1", "%", "+/-", "4.0", "%", ",", "P", "=.009", ")", "."]} {"tags": [3, 0, 0, 5, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0, 5, 0], "tokens": ["GRbeta", "expression", "in", "NPs", "was", "almost", "exclusive", "to", "T", "lymphocytes", ",", "eosinophils", ",", "and", "macrophages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 6, 0, 0, 3, 0, 0, 0, 5, 6, 6, 0], "tokens": ["An", "inverse", "correlation", "was", "observed", "between", "the", "baseline", "inflammatory", "cell", "GRbeta", "expression", "and", "the", "reduction", "after", "FP", "treatment", "in", "EG2-positive", "eosinophils", ",", "CD4-positive", "T", "lymphocytes", ",", "endothelial", "VCAM-1", "expression", ",", "and", "IL-4", "mRNA-positive", "cells", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NPs", "that", "were", "``", "FP-insensitive", "''", "in", "terms", "of", "suppression", "of", "eosinophil", "numbers", "(", "major", "basic", "protein", "-positive", ")", "had", "a", "significantly", "greater", "percentage", "of", "GRbeta-positive", "inflammatory", "cells", ",", "a", "higher", "ratio", "of", "GRbeta-positive/GRalpha-positive", "cells", ",", "and", "increased", "numbers", "of", "GRbeta-positive", "eosinophils", "and", "macrophages", "in", "comparison", "with", "those", "that", "were", "``", "FP-sensitive.", "''"]} {"tags": [5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["``", "FP-insensitive", "''", "NPs", "also", "demonstrated", "a", "higher", "percentage", "of", "IL-5-positive", "inflammatory", "cells", "expressing", "GRbeta", "before", "and", "after", "FP", "treatment", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["CONCLUSION", ":", "GRbeta", "expression", "appears", "to", "be", "a", "marker", "of", "steroid", "insensitivity", "in", "NPs", "."]} {"tags": [0, 0, 3, 0, 5, 6, 6, 0, 0, 5, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["Expression", "of", "GRbeta", "by", "NP", "inflammatory", "cells", ",", "particularly", "T", "cells", "and", "eosinophils", ",", "might", "render", "them", "resistant", "to", "suppression", "by", "topical", "steroids", "and", "thereby", "contribute", "to", "persistent", "NP", "inflammation", "."]} {"tags": [0, 0, 0, 3, 0, 3, 4, 4, 4, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transcriptional", "regulation", "of", "galectin-10", "(", "eosinophil", "Charcot-Leyden", "crystal", "protein", ")", ":", "a", "GC", "box", "(", "-44", "to", "-50", ")", "controls", "butyric", "acid", "induction", "of", "gene", "expression", "."]} {"tags": [3, 0, 3, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 4, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Galectin-10", "(", "gal-10", ",", "also", "known", "as", "Charcot-Leyden", "crystal", "protein", ")", "is", "a", "member", "of", "the", "galectin", "family", "of", "beta-galactoside", "binding", "proteins", "that", "is", "expressed", "uniquely", "in", "eosinophilic", "and", "basophilic", "leukocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["To", "gain", "a", "better", "understanding", "of", "galectin", "gene", "expression", ",", "we", "present", "an", "analysis", "of", "the", "transcriptional", "regulation", "of", "the", "gene", "encoding", "gal-10", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 2, 2, 2, 2, 2, 0], "tokens": ["Analysis", "of", "the", "minimal", "promoter", "revealed", "nine", "consensus-binding", "sites", "for", "transcription", "factors", ",", "including", "several", "that", "are", "also", "found", "in", "the", "minimal", "promoters", "of", "galectins", "-1", ",", "-2", ",", "and", "-3", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "decrease", "in", "gal-10", "promoter", "activity", "after", "disruption", "of", "either", "the", "GC", "box", "(", "-44", "to", "-50", ")", "or", "the", "Oct", "site", "(", "-255", "to", "-261", ")", "suggests", "that", "these", "sites", ",", "along", "with", "the", "previously", "characterized", "GATA", "and", "EoTF", "sites", ",", "are", "necessary", "for", "full", "promoter", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 0, 3, 0, 0, 1, 2, 2, 0, 0, 1, 2, 0, 0, 0], "tokens": ["By", "supershift", "analysis", ",", "we", "demonstrate", "binding", "of", "the", "transcription", "factors", "Sp1", "and", "Oct1", "to", "the", "consensus", "GC", "box", "and", "the", "Oct", "site", ",", "respectively", "."]} {"tags": [0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Similar", "to", "gal-1", ",", "gal-10", "expression", "is", "induced", "by", "butyric", "acid", ",", "an", "effect", "that", "is", "lost", "upon", "ablation", "of", "the", "GC", "box", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 3, 4, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Additionally", ",", "we", "demonstrate", "AML3", "binding", "to", "the", "consensus", "AML", "site", "and", "YY1", "binding", "to", "the", "Inr", "sequence", ",", "both", "elements", "functioning", "as", "silencers", "in", "the", "gal-10", "promoter", "."]} {"tags": [0, 0, 0], "tokens": ["Tumour-stromal", "interactions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Role", "of", "the", "stroma", "in", "mammary", "development", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mammary", "development", "depends", "on", "branching", "morphogenesis", ",", "namely", "the", "bifurcation", "and", "extension", "of", "ductal", "growth", "points", "(", "end", "buds", ")", "and", "secretory", "lobules", "into", "a", "more", "or", "less", "fatty", "stroma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Because", "breast", "carcinomas", "are", "overwhelmingly", "ductal", "in", "origin", ",", "this", "review", "focuses", "on", "stromal", "influences", "guiding", "postnatal", "ductal", "development", "and", "there", "is", "only", "the", "briefest", "account", "of", "the", "role", "of", "embryonic", "stroma", "(", "mesenchyme", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "stroma", "as", "the", "necessary", "target", "for", "endocrine", "mammogens", "and", "the", "source", "of", "stimulatory", "growth", "factors", "is", "described", "and", "the", "importance", "of", "mammary", "epithelium-induced", "modifications", "of", "the", "periductal", "stroma", "is", "emphasized", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Evidence", "is", "presented", "that", "if", "they", "are", "to", "grow", ",", "end", "buds", "must", "condition", "proximal", "fatty", "stroma", "by", "recruiting", "white", "blood", "cells", "as", "well", "as", "inducing", "stromal", "cell", "division", "and", ",", "possibly", ",", "estrogen", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "induction", "of", "a", "fibrous", "stromal", "tunic", "around", "the", "end", "bud", "is", "described", "and", "its", "likely", "role", "as", "a", "complex", "ductal", "morphogen", "is", "discussed", ";", "a", "possible", "role", "in", "growth", "inhibition", "is", "also", "considered", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "the", "signals", "governing", "fibrotic", "induction", ",", "ductal", "morphogenesis", ",", "and", "growth", "inhibition", "are", "unknown", ",", "a", "role", "for", "transforming", "growth", "factor-beta", "is", "highly", "likely", "and", "is", "discussed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Finally", ",", "a", "need", "for", "new", "conceptual", "and", "experimental", "approaches", "to", "understanding", "stromal-epithelial", "signaling", "is", "discussed", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Adipophilin", "is", "a", "sensitive", "marker", "for", "lipid", "loading", "in", "human", "blood", "monocytes", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Adipophilin", ",", "a", "marker", "of", "lipid", "accumulation", "initially", "described", "in", "adipocytes", ",", "was", "recently", "shown", "to", "be", "induced", "in", "macrophage", "foam", "cells", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 5, 0, 0, 0, 0, 0], "tokens": ["We", "found", "that", "even", "freshly", "isolated", "blood", "monocytes", "express", "adipophilin", "and", "that", "the", "amount", "of", "adipophilin", "protein", "is", "variable", "in", "monocytes", "from", "different", "healthy", "individuals", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "physiological", "expression", "of", "adipophilin", "does", "not", "correlate", "with", "the", "levels", "of", "free", "fatty", "acids", ",", "cholesterylesters", "or", "free", "cholesterol", "."]} {"tags": [3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Enzymatically", "modified", "low-density", "lipoprotein", "(", "E-LDL", ")", "induces", "rapid", "foam", "cell", "formation", "in", "monocytes", "and", "upregulates", "adipophilin", "mRNA", "and", "protein", "within", "2", "h", "of", "incubation", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 3, 0], "tokens": ["This", "rapid", "induction", "of", "adipophilin", "is", "accompanied", "by", "a", "significant", "increase", "of", "free", "fatty", "acids", "in", "monocytes", "incubated", "with", "E-LDL", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Adipophilin", "facilitates", "the", "uptake", "of", "free", "fatty", "acids", ",", "and", "here", "we", "demonstrate", "that", "free", "fatty", "acids", "increase", "is", "related", "to", "the", "early", "upregulation", "of", "adipophilin", "expression", "in", "blood", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 9, 10, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Fatty", "acids", "are", "ligands", "for", "peroxisome", "proliferator-activated", "receptor-gamma", "(", "PPARgamma", ")", ",", "and", "the", "upregulation", "of", "adipophilin", "mRNA", "by", "PPARgamma", "agonists", "like", "15d-PGJ", "(", "2", ")", "and", "ciglitazone", "indicates", "that", "PPARgamma", "may", "mediate", "the", "induction", "of", "adipophilin", "expression", "in", "human", "blood", "monocytes", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 7, 8, 8, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Constitutive", "expression", "of", "MHC", "class", "II", "genes", "in", "melanoma", "cell", "lines", "results", "from", "the", "transcription", "of", "class", "II", "transactivator", "abnormally", "initiated", "from", "its", "B", "cell-specific", "promoter", "."]} {"tags": [0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["In", "melanoma", "cell", "lines", ",", "two", "different", "patterns", "of", "MHC", "class", "II", "expression", "have", "been", "described", ",", "either", "an", "IFN", "gamma-inducible", "expression", "of", "HLA-DR", "and", "HLA-DP", ",", "with", "a", "faint", "or", "null", "expression", "of", "HLA-DQ", ",", "resembling", "that", "described", "for", "melanocytes", ",", "or", "a", "constitutive", "expression", ",", "i.e.", ",", "IFN-gamma", "independent", ",", "of", "all", "three", "HLA-D", "isotypes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["As", "this", "latter", "phenotype", "has", "been", "associated", "with", "a", "more", "rapid", "progression", "of", "melanoma", "tumors", ",", "we", "have", "analyzed", "in", "different", "melanoma", "cell", "lines", "the", "molecular", "mechanisms", "leading", "to", "this", "abnormal", "pattern", "of", "MHC", "class", "II", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "agreement", "with", "the", "evidence", "of", "a", "coordinate", "transcription", "of", "the", "HLA-D", "genes", "in", "these", "cell", "lines", ",", "we", "have", "shown", "the", "constitutive", "expression", "of", "CIITA", "(", "class", "II", "transactivator", ")", "transcripts", ",", "CIITA", "being", "known", "as", "the", "master", "switch", "of", "MHC", "class", "II", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Unexpectedly", ",", "these", "transcripts", "initiate", "from", "promoter", "III", "of", "the", "CIITA", "gene", ",", "a", "promoter", "that", "is", "mainly", "used", "constitutively", "in", "B", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["This", "expression", "was", "further", "shown", "to", "occur", "through", "factor", "(", "s", ")", "acting", "on", "the", "enhancer", "located", "upstream", "of", "CIITA", "promoter", "III", ",", "which", "was", "previously", "described", "in", "epithelioid", "cells", "as", "an", "IFN-gamma-response", "sequence", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "hypothesis", "of", "a", "general", "abnormality", "of", "the", "IFN-gamma", "transduction", "pathway", "was", "dismissed", "."]} {"tags": [0, 0, 0, 1, 0, 1, 2, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6], "tokens": ["Constitutive", "transcription", "of", "CIITA", "from", "promoter", "III", "having", "been", "observed", "in", "unrelated", "melanoma", "cell", "lines", ",", "we", "propose", "the", "hypothesis", "that", "this", "phenomenon", "might", "not", "be", "a", "random", "event", ",", "but", "could", "be", "linked", "to", "the", "neoplasic", "state", "of", "the", "melanoma", "cells"]} {"tags": [3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Caspase", "-mediated", "calcineurin", "activation", "contributes", "to", "IL-2", "release", "during", "T", "cell", "activation", "."]} {"tags": [3, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Calcineurin", ",", "a", "Ca", "(", "2+", ")", "/calmodulin-dependent", "Ser/Thr", "phosphatase", "(", "protein", "phosphatase", "2B", ")", ",", "plays", "a", "critical", "role", "in", "IL-2", "production", "during", "T", "cell", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "has", "been", "previously", "reported", "that", "IL-2", "release", "in", "activated", "Jurkat", "T", "requires", "caspase-like", "activity", "(", "Posmantur", "et", "al.", "(", "1998", ")", "Exp.", "Cell.", "Res.", "244", ",", "302-309", ")", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["We", "report", "here", "that", "the", "60-kDa", "catalytic", "subunit", "of", "calcineurin", "A", "(", "Cn", "A", ")", "was", "partially", "cleaved", "to", "a", "45-kDa", "form", "in", "phytohemagglutinin", "A", "(", "PHA", ")", "or", "phorbol", "ester", "+", "ionomycin", "(", "P", "+", "I", ")", "-activated", "Jurkat", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["In", "parallel", ",", "proteolytic", "activation", "of", "upstream", "caspases", "(", "caspase-8", "and", "-9", ")", "as", "well", "as", "effector", "caspase-3", "was", "also", "observed", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cn", "A", "cleavage", "was", "caspase", "mediated", ",", "since", "it", "was", "inhibitable", "by", "pan-caspase", "inhibitor", "Cbz-Asp-CH", "(", "2", ")", "OC", "(", "O", ")", "-2", ",", "6-dichlorobenzene", "(", "Z-D-DCB", ")", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Cn", "A", "cleavage", "was", "also", "observed", "when", "purified", "calcineurin", "was", "digested", "in", "vitro", "with", "caspase-3", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Truncated", "Cn", "A", "was", "associated", "with", "enhanced", "phosphatase", "activity", "and", "reduced", "calmodulin", "sensitivity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 3, 0, 3, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "in", "PHA", "or", "P", "+", "I-activated", "Jurkat", "cells", ",", "dephosphorylation", "of", "calcineurin", "substrate", "NFATc", "(", "a", "transcription", "factor", "known", "to", "be", "involved", "in", "transactivation", "of", "the", "IL-2", "gene", ")", ",", "was", "also", "suppressed", "by", "Z-D-DCB", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Taken", "together", ",", "our", "results", "suggest", "that", "caspase-mediated", "cleavage", "of", "Cn", "A", "contributes", "to", "IL-2", "production", "during", "T", "cell", "activation", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Copyright", "2001", "Academic", "Press", "."]} {"tags": [0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["Maturation", "of", "human", "dendritic", "cells", "as", "sulfasalazine", "target", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["AIM", ":", "Sulfasalazine", ",", "a", "nonsteroidal", "anti-inflammatory", "drug", ",", "is", "effective", "in", "treating", "some", "autoimmune", "diseases", ",", "but", "its", "mechanism", "of", "action", "is", "unclear", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["To", "determine", "whether", "dendritic", "cells", "could", "be", "a", "possible", "target", "of", "the", "drug", ",", "we", "studied", "the", "effects", "of", "sulfasalazine", "and", "its", "metabolites", ",", "aminosalicylate", "and", "sulfapyridine", ",", "on", "in", "vitro", "maturation", "(", "terminal", "differentiation", ")", "of", "human", "myeloid", "dendritic", "cells", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 0, 0, 5, 6, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 4, 4, 4, 0], "tokens": ["METHODS", ":", "We", "prepared", "immature", "dendritic", "cells", "by", "incubating", "CD14-positive", "cells", "in", "the", "presence", "of", "granulocyte-", "macrophage", "colony-stimulating", "factor", "and", "interleukin", "(", "IL", ")", "-4", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "cells", "were", "matured", "by", "addition", "of", "tumor", "necrosis", "factor", "(", "TNF", ")", "-a", ",", "IL-1", "beta", ",", "and", "prostaglandin", "E2", "in", "the", "presence", "of", "sulfasalazine", "or", "its", "metabolites", "--", "aminosalicylate", "and", "sulfapyridine", ",", "or", "their", "combinations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0], "tokens": ["We", "quantified", "the", "effect", "of", "drugs", "on", "the", "dendritic", "cell", "characteristics", ",", "such", "as", "stimulation", "of", "autologous", "and", "allogeneic", "pan-T", "cell", "proliferation", ",", "surface", "marker", "phenotype", ",", "IL-12", "p40", "subunit", "secretion", ",", "and", "activation", "of", "nuclear", "transcription", "factor", "(", "NF", ")", "-kappa", "B", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "Dendritic", "cells", "treated", "with", "sulfasalazine", "(", "1.25", "micromol/L", "or", "2.5", "micromol/L", ")", "could", "not", "stimulate", "T", "cells", "(", "p", "<", "0.028", ",", "two-sided", "paired", "t-test", ")", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "distinction", "to", "drug-free", "maturing", "dendritic", "cells", ",", "2.5", "micromol/L", "sulfasalazine", "upregulated", "the", "levels", "of", "CD14", "and", "CD68", "and", "downregulated", "the", "levels", "of", "CD40", ",", "CD80", ",", "and", "CD83", "(", "for", "all", "CD", "markers", ",", "p", "<", "0.03", "for", "difference", "between", "measurements", "in", "the", "absence", "and", "the", "presence", "of", "sulfasalazine", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["From", "concentration-dependent", "changes", "in", "CD83", "expression", ",", "we", "found", "an", "apparent", "ID50", ">", ">", "1.5", "micromol/L", "sulfasalazine", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "apparent", "ID50", "value", "for", "aminosalicylate-inhibited", "maturation", "was", "4", "micromol/L", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Sulfapyridine", "had", "no", "effect", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["At", "1.25", "micromol/L", ",", "sulfasalazine", "largely", "inhibited", "NF-kB", "activation", "in", "dendritic", "cells", "."]} {"tags": [0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSION", ":", "Maturing", "human", "dendritic", "cells", "are", "hundred-fold", "more", "sensitive", "to", "sulfasalazine", "than", "T", "cells", "and", "NK", "cells", "and", "the", "most", "sensitive", "human", "cells", "described", "so", "far", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "dendritic", "cell", "maturation", "is", "an", "important", "target", "of", "sulfasalazine", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Because", "of", "the", "role", "of", "dendritic", "cells", "in", "(", "auto", ")", "immunity", ",", "inhibition", "of", "their", "maturation", "might", "provide", "a", "target", "for", "further", "optimization", "of", "sulfasalazine", "therapy", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Defective", "function", "of", "the", "proteasome", "in", "autoimmunity", ":", "involvement", "of", "impaired", "NF-kappaB", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Type", "1", "diabetes", "(", "also", "known", "as", "insulin-dependent", "diabetes", "mellitus", "or", "juvenile-onset", "diabetes", ")", "is", "usually", "caused", "by", "T", "cell-mediated", "autoimmunity", ",", "with", "a", "prediabetic", "state", "characterized", "by", "the", "production", "of", "autoantibodies", "specific", "for", "proteins", "expressed", "by", "pancreatic", "beta", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 1, 0], "tokens": ["The", "nonobese", "patient", "with", "diabetes", "(", "NOD", ")", "mouse", "is", "a", "spontaneous", "model", "of", "type", "1", "diabetes", "with", "a", "strong", "genetic", "component", "that", "maps", "to", "the", "major", "histocompatibility", "complex", "(", "MHC", ")", "region", "of", "the", "genome", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["A", "specific", "proteasome", "defect", "has", "been", "identified", "in", "NOD", "mouse", "in", "select", "lymphocytic", "and", "monocytic", "lineages", "that", "results", "from", "down-regulation", "of", "expression", "of", "the", "proteasome", "subunit", "LMP2", ",", "which", "is", "encoded", "by", "a", "gene", "in", "the", "MHC", "genomic", "region", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0], "tokens": ["This", "defect", "prevents", "the", "proteolytic", "processing", "required", "for", "the", "production", "and", "activation", "of", "the", "transcription", "factor", "nuclear", "factor-kappaB", "(", "NF-kappaB", ")", ",", "which", "plays", "important", "roles", "in", "immune", "and", "inflammatory", "responses", ",", "as", "well", "as", "increases", "the", "susceptibility", "of", "the", "affected", "cells", "to", "apoptosis", "induced", "by", "tumor", "necrosis", "factor-alpha", "(", "TNF-alpha", ")", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "novel", "role", "of", "the", "proteasome", "in", "dysfunction", "in", "autoimmunity", "is", "presented", "and", "documented", "to", "be", "both", "tissue", "and", "developmental", "stage", "specific", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "propose", "a", "role", "of", "the", "proteasome", "as", "a", "step", "in", "disease", "pathogenesis", "and", "tissue", "targeting", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Down-regulation", "of", "TDT", "transcription", "in", "CD4", "(", "+", ")", "CD8", "(", "+", ")", "thymocytes", "by", "Ikaros", "proteins", "in", "direct", "competition", "with", "an", "Ets", "activator", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ikaros", "is", "a", "unique", "regulator", "of", "lymphopoiesis", "that", "associates", "with", "pericentromeric", "heterochromatin", "and", "has", "been", "implicated", "in", "heritable", "gene", "inactivation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Binding", "and", "competition", "experiments", "demonstrate", "that", "Ikaros", "dimers", "compete", "with", "an", "Ets", "activator", "for", "occupancy", "of", "the", "lymphocyte-specific", "TdT", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 8, 0], "tokens": ["Mutations", "that", "selectively", "disrupt", "Ikaros", "binding", "to", "an", "integrated", "TdT", "promoter", "had", "no", "effect", "on", "promoter", "function", "in", "a", "CD4", "(", "+", ")", "CD8", "(", "+", ")", "thymocyte", "line", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "these", "mutations", "abolished", "down-regulation", "on", "differentiation", ",", "providing", "evidence", "that", "Ikaros", "plays", "a", "direct", "role", "in", "repression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Reduced", "access", "to", "restriction", "enzyme", "cleavage", "suggested", "that", "chromatin", "alterations", "accompany", "down-regulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "Ikaros-dependent", "down-regulation", "event", "and", "the", "observed", "chromatin", "alterations", "appear", "to", "precede", "pericentromeric", "repositioning", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 0], "tokens": ["Current", "models", "propose", "that", "the", "functions", "of", "Ikaros", "should", "be", "disrupted", "by", "a", "small", "isoform", "that", "retains", "the", "dimerization", "domain", "and", "lacks", "the", "DNA-binding", "domain", "."]} {"tags": [0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 8, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Surprisingly", ",", "in", "the", "CD4", "(", "+", ")", "CD8", "(", "+", ")", "thymocyte", "line", ",", "overexpression", "of", "a", "small", "Ikaros", "isoform", "had", "no", "effect", "on", "differentiation", "or", "on", "the", "pericentromeric", "targeting", "and", "DNA-binding", "properties", "of", "Ikaros", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 3, 4, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Rather", ",", "the", "small", "isoform", "assembled", "into", "multimeric", "complexes", "with", "DNA-bound", "Ikaros", "at", "the", "pericentromeric", "foci", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 1, 2, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["The", "capacity", "for", "in", "vivo", "multimer", "formation", "suggests", "that", "interactions", "between", "Ikaros", "dimers", "bound", "to", "the", "TdT", "promoter", "and", "those", "bound", "to", "pericentromeric", "repeat", "sequences", "may", "contribute", "to", "the", "pericentromeric", "repositioning", "of", "the", "inactive", "gene", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Type", "I", "interferons", "and", "IL-12", ":", "convergence", "and", "cross-regulation", "among", "mediators", "of", "cellular", "immunity", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Therapeutic", "use", "of", "type", "I", "IFN", "(", "IFN-alpha/beta", ")", "has", "become", "common", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Many", "of", "the", "diverse", "diseases", "targeted", "are", "marked", "by", "pathogenetic", "abnormalities", "in", "cell-mediated", "immunity", "(", "CMI", ")", ",", "these", "cellular", "immune", "responses", "either", "causing", "injury", "to", "the", "host", ",", "lacking", "sufficient", "vigor", "for", "virus", "or", "tumor", "clearance", ",", "or", "both", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "general", ",", "therapeutic", "efficacy", "is", "limited", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["It", "is", "thus", "notable", "that", "the", "pleiotropic", "effects", "of", "type", "I", "IFN", "on", "CMI", "remain", "poorly", "understood", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0], "tokens": ["We", "characterized", "the", "effects", "of", "type", "I", "IFN", "on", "the", "production", "of", "IL-12", ",", "the", "central", "immunoregulatory", "cytokine", "of", "the", "CD4", "(", "+", ")", "T", "cell", "arm", "of", "CMI", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["We", "show", "that", "type", "I", "IFN", "are", "potent", "inhibitors", "of", "IL-12", "production", "by", "human", "monocytes/macrophages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 0], "tokens": ["The", "underlying", "mechanism", "involves", "transcriptional", "inhibition", "of", "the", "IL-12p40", "gene", ",", "marked", "by", "down-regulation", "of", "PU.1", "binding", "activity", "at", "the", "upstream", "Ets", "site", "of", "the", "IL-12p40", "promoter", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Type", "I", "IFN", "have", "previously", "been", "shown", "to", "be", "able", "to", "substitute", "for", "IL-12", "in", "driving", "IFN-gamma", "production", "from", "T", "and", "NK", "cells", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "ability", "of", "IFN-alpha/beta", "to", "suppress", "IL-12", "production", "while", "up-regulating", "IFN-gamma", "production", "suggests", "a", "possible", "mechanistic", "basis", "for", "the", "difficulties", "of", "employing", "these", "cytokines", "in", "diseases", "involving", "abnormalities", "of", "CMI", "."]} {"tags": [3, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nuclear", "factor-kappaB", "suppressive", "and", "inhibitor-kappaB", "stimulatory", "effects", "of", "troglitazone", "in", "obese", "patients", "with", "type", "2", "diabetes", ":", "evidence", "of", "an", "antiinflammatory", "action", "?"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "has", "been", "shown", "recently", "that", "troglitazone", "exerts", "an", "anti-inflammatory", "effect", ",", "in", "vitro", ",", "and", "in", "experimental", "animals", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 3, 4, 4, 0, 5, 6, 0, 5, 0, 0, 3, 4, 4, 4, 4, 0, 3, 4, 4, 0, 3, 4, 4, 0, 0, 3, 4, 0], "tokens": ["To", "test", "these", "properties", "in", "humans", ",", "we", "investigated", "the", "effect", "of", "troglitazone", "on", "the", "proinflammatory", "transcription", "factor", "nuclear", "factor-kappaB", "and", "its", "inhibitory", "protein", "IkappaB", "in", "mononuclear", "cells", "(", "MNC", ")", "and", "plasma", "soluble", "intracellular", "adhesion", "molecule-1", ",", "monocyte", "chemoattractant", "protein-1", ",", "plasminogen", "activator", "inhibitor-1", ",", "and", "C-reactive", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "also", "examined", "the", "effect", "of", "troglitazone", "on", "reactive", "oxygen", "species", "generation", ",", "p47", "(", "phox", ")", "subunit", "expression", ",", "9-hydroxyoctadecadienoic", "acid", "(", "9-HODE", ")", ",", "13-HODE", ",", "o-tyrosine", ",", "and", "m-tyrosine", "in", "obese", "patients", "with", "type", "2", "diabetes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Seven", "obese", "patients", "with", "type", "2", "diabetes", "were", "treated", "with", "troglitazone", "(", "400", "mg/day", ")", "for", "4", "weeks", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Blood", "samples", "were", "obtained", "at", "weekly", "intervals", "."]} {"tags": [3, 4, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nuclear", "factor-kappaB", "binding", "activity", "in", "MNC", "nuclear", "extracts", "was", "significantly", "inhibited", "after", "troglitazone", "treatment", "at", "week", "1", "and", "continued", "to", "be", "inhibited", "up", "to", "week", "4", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["On", "the", "other", "hand", ",", "IkappaB", "protein", "levels", "increased", "significantly", "after", "troglitazone", "treatment", "at", "week", "1", ",", "and", "this", "increase", "persisted", "throughout", "the", "study", "."]} {"tags": [3, 4, 4, 4, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Plasma", "monocyte", "chemoattractant", "protein-1", "and", "soluble", "intracellular", "adhesion", "molecule-1", "concentrations", "did", "not", "decrease", "significantly", "after", "troglitazone", "treatment", ",", "although", "there", "was", "a", "trend", "toward", "inhibition", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 3, 4, 4, 4, 4, 4, 0, 0, 3, 4, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Reactive", "oxygen", "species", "generation", "by", "polymorphonuclear", "cells", "and", "MNC", ",", "p47", "(", "phox", ")", "subunit", "protein", "quantities", ",", "plasminogen", "activator", "inhibitor-1", ",", "and", "C-reactive", "protein", "levels", "decreased", "significantly", "after", "troglitazone", "intake", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["13-HODE/linoleic", "acid", "and", "9-HODE/linoleic", "acid", "ratios", "also", "decreased", "after", "troglitazone", "intake", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "o-tyrosine/phenylalanine", "and", "m-tyrosine/phenylalanine", "ratios", "did", "not", "change", "significantly", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "show", "that", "troglitazone", "has", "profound", "antiinflammatory", "effects", "in", "addition", "to", "antioxidant", "effects", "in", "obese", "type", "2", "diabetics", ";", "these", "effects", "may", "be", "relevant", "to", "the", "recently", "described", "beneficial", "antiatherosclerotic", "effects", "of", "troglitazone", "at", "the", "vascular", "level", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["OX40", "stimulation", "by", "gp34/OX40", "ligand", "enhances", "productive", "human", "immunodeficiency", "virus", "type", "1", "infection", "."]} {"tags": [3, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 6, 0], "tokens": ["OX40", "is", "a", "member", "of", "the", "tumor", "necrosis", "factor", "(", "TNF", ")", "receptor", "superfamily", "and", "known", "to", "be", "an", "important", "costimulatory", "molecule", "expressed", "on", "activated", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 7, 8, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0], "tokens": ["To", "investigate", "the", "role", "of", "costimulation", "of", "OX40", "in", "human", "immunodeficiency", "virus", "type", "1", "(", "HIV-1", ")", "infection", "by", "its", "natural", "ligand", ",", "gp34", ",", "the", "OX40-transfected", "ACH-2", "cell", "line", ",", "ACH-2/OX40", ",", "chronically", "infected", "with", "HIV-1", ",", "was", "cocultured", "with", "paraformaldehyde", "(", "PFA", ")", "-fixed", "gp34-transfected", "mouse", "cell", "line", ",", "SV-T2/gp34", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "results", "showed", "that", "HIV-1", "production", "was", "strongly", "induced", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 4, 0], "tokens": ["This", "was", "followed", "by", "apparent", "apoptosis", ",", "and", "both", "processes", "were", "specifically", "inhibited", "by", "the", "gp34", "-specific", "neutralizing", "monoclonal", "antibody", "5A8", "."]} {"tags": [0, 3, 4, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Endogenous", "TNF", "alpha", "(", "TNF-alpha", ")", "and", "TNF-beta", "production", "were", "not", "involved", "in", "the", "enhanced", "HIV-1", "production", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 7, 8, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Furthermore", ",", "enhanced", "HIV-1", "transcription", "in", "gp34", "-stimulated", "ACH-2/OX40", "cells", "was", "dependent", "on", "the", "kappa", "B", "site", "of", "the", "HIV-1", "long", "terminal", "repeat", ",", "and", "the", "OX40-gp34", "interaction", "activated", "NF-kappa", "B", "consisting", "of", "p50", "and", "p65", "subunits", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 7, 8, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["When", "primary", "activated", "CD4", "(", "+", ")", "T", "cells", "acutely", "infected", "with", "HIV-1", "(", "NL4-3", ")", "(", "CXCR4-using", "T-cell-line-tropic", ")", "were", "cocultured", "with", "PFA-fixed", "gp34", "(", "+", ")", "human", "T-cell", "leukemia", "virus", "type", "1-bearing", "MT-2", "cells", "or", "SV-T2/gp34", "cells", ",", "HIV-1", "production", "was", "also", "markedly", "enhanced", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["The", "enhancement", "was", "again", "significantly", "inhibited", "by", "5A8", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0], "tokens": ["The", "present", "study", "first", "shows", "that", "OX40-gp34", "interaction", "stimulates", "HIV-1", "expression", "and", "suggests", "that", "OX40", "triggering", "by", "gp34", "may", "play", "an", "important", "role", "in", "enhancing", "HIV-1", "production", "in", "both", "acutely", "and", "latently", "infected", "CD4", "(", "+", ")", "T", "cells", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Troglitazone", ",", "a", "PPARgamma", "ligand", ",", "inhibits", "osteopontin", "gene", "expression", "in", "human", "monocytes/macrophage", "THP-1", "cells", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Peroxizome", "proliferator-activated", "receptor-gamma", "(", "PPARgamma", ")", "is", "a", "member", "of", "the", "nuclear", "receptor", "family", "of", "transcription", "factors", "that", "regulate", "adipocyte", "differentiation", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["Recent", "studies", "indicate", "that", "liganded", "PPARgamma", "not", "only", "promotes", "differentiation", "but", "also", "inhibits", "the", "activation", "of", "macrophages", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["Osteopontin", ",", "a", "component", "of", "extracellular", "matrix", ",", "is", "synthesized", "by", "macrophages", "in", "atherosclerotic", "plaques", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", ",", "we", "examined", "whether", "PPARgamma", "ligand", "regulates", "osteopontin", "gene", "expression", "in", "THP-1", "cells", ",", "a", "cell", "line", "derived", "from", "human", "monocytic", "leukemia", "cells", "which", "can", "differentiate", "to", "macrophage", "upon", "stimulation", "with", "phorbol", "ester", "PMA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Northern", "blot", "analysis", "showed", "that", "osteopontin", "expression", "is", "markedly", "induced", "in", "response", "to", "PMA", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Troglitazone", ",", "a", "PPARgamma", "ligand", ",", "dramatically", "attenuated", "the", "PMA-induced", "osteopontin", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0], "tokens": ["Transient", "transfection", "assays", "of", "the", "human", "osteopontin", "promoter/luciferase", "construct", "which", "contains", "a", "5'-flanking", "region", "between", "-1500", "and", "+87", "relative", "to", "the", "transcription", "start", "site", "demonstrate", "that", "either", "treatment", "with", "troglitazone", "or", "cotransfection", "of", "PPARgamma", "expression", "vector", "inhibits", "osteopontin", "promoter", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "indicate", "that", "troglitazone", "reduces", "osteopontin", "gene", "expression", "at", "transcriptional", "level", "through", "PPARgamma", "activation", ",", "and", "suggest", "the", "role", "of", "troglitazone", "in", "inhibiting", "the", "ability", "of", "macrophages", "to", "produce", "extracellular", "matrix", ",", "which", "is", "particularly", "relevant", "to", "atherosclerotic", "plaque", "formation", "."]} {"tags": [3, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Transcription", "factor", "NF-kappa", "B", "regulates", "Ig", "lambda", "light", "chain", "gene", "rearrangement", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 5, 6, 0], "tokens": ["The", "tissue-", "and", "stage-specific", "assembly", "of", "Ig", "and", "TCR", "genes", "is", "mediated", "by", "a", "common", "V", "(", "D", ")", "J", "recombinase", "complex", "in", "precursor", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 3, 0, 0, 1, 2, 2, 0], "tokens": ["Directed", "alterations", "in", "the", "accessibility", "of", "V", ",", "D", ",", "and", "J", "gene", "segments", "target", "the", "recombinase", "to", "specific", "Ag", "receptor", "loci", "."]} {"tags": [0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Accessibility", "within", "a", "given", "locus", "is", "regulated", "by", "the", "functional", "interaction", "of", "transcription", "factors", "with", "cognate", "enhancer", "elements", "and", "correlates", "with", "the", "transcriptional", "activity", "of", "unrearranged", "gene", "segments", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 3, 4, 4, 3, 0], "tokens": ["As", "demonstrated", "in", "our", "prior", "studies", ",", "rearrangement", "of", "the", "Igkappa", "locus", "is", "regulated", "by", "the", "inducible", "transcription", "factor", "NF-kappaB", "."]} {"tags": [0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 0, 0, 1, 2, 0, 0, 1, 2, 2, 0], "tokens": ["In", "contrast", "to", "the", "Igkappa", "locus", ",", "known", "transcriptional", "control", "elements", "in", "the", "Iglambda", "locus", "lack", "functional", "NF-kappaB", "binding", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 6, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Consistent", "with", "this", "observation", ",", "the", "expression", "of", "assembled", "Iglambda", "genes", "in", "mature", "B", "cells", "has", "been", "shown", "to", "be", "NF-kappaB", "independent", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 5, 6, 6, 0], "tokens": ["Nonetheless", ",", "we", "now", "show", "that", "specific", "repression", "of", "NF-kappaB", "inhibits", "germline", "transcription", "and", "recombination", "of", "Iglambda", "gene", "segments", "in", "precursor", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Molecular", "analyses", "indicate", "that", "the", "block", "in", "NF-kappaB", "impairs", "Iglambda", "rearrangement", "at", "the", "level", "of", "recombinase", "accessibility", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "the", "activities", "of", "known", "Iglambda", "promoter", "and", "enhancer", "elements", "are", "unaffected", "in", "the", "same", "cellular", "background", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 6, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["These", "findings", "expand", "the", "range", "of", "NF-kappaB", "action", "in", "precursor", "B", "cells", "beyond", "Igkappa", "to", "include", "the", "control", "of", "recombinational", "accessibility", "at", "both", "L", "chain", "loci", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "our", "results", "strongly", "suggest", "the", "existence", "of", "a", "novel", "Iglambda", "regulatory", "element", "that", "is", "either", "directly", "or", "indirectly", "activated", "by", "NF-kappaB", "during", "the", "early", "stages", "of", "B", "cell", "development", "."]} {"tags": [3, 4, 3, 0, 0, 0, 0, 3, 4, 4, 4, 0, 5, 6, 0], "tokens": ["Transcription", "factor", "STAT5A", "is", "a", "substrate", "of", "Bruton", "'s", "tyrosine", "kinase", "in", "B", "cells", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["STAT5A", "is", "a", "molecular", "regulator", "of", "proliferation", ",", "differentiation", ",", "and", "apoptosis", "in", "lymphohematopoietic", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0], "tokens": ["Here", "we", "show", "that", "STAT5A", "can", "serve", "as", "a", "functional", "substrate", "of", "Bruton", "'s", "tyrosine", "kinase", "(", "BTK", ")", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Purified", "recombinant", "BTK", "was", "capable", "of", "directly", "binding", "purified", "recombinant", "STAT5A", "with", "high", "affinity", "(", "K", "(", "d", ")", "=", "44", "nm", ")", ",", "as", "determined", "by", "surface", "plasmon", "resonance", "using", "a", "BIAcore", "biosensor", "system", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0], "tokens": ["BTK", "was", "also", "capable", "of", "tyrosine-phosphorylating", "ectopically", "expressed", "recombinant", "STAT5A", "on", "Tyr", "(", "694", ")", "both", "in", "vitro", "and", "in", "vivo", "in", "a", "Janus", "kinase", "3", "-independent", "fashion", "."]} {"tags": [3, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 3, 4, 0, 3, 0], "tokens": ["BTK", "phosphorylated", "the", "Y665F", ",", "Y668F", ",", "and", "Y682F", ",", "Y683F", "mutants", "but", "not", "the", "Y694F", "mutant", "of", "STAT5A", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["STAT5A", "mutations", "in", "the", "Src", "homology", "2", "(", "SH2", ")", "and", "SH3", "domains", "did", "not", "alter", "the", "BTK", "-mediated", "tyrosine", "phosphorylation", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Recombinant", "BTK", "proteins", "with", "mutant", "pleckstrin", "homology", ",", "SH2", ",", "or", "SH3", "domains", "were", "capable", "of", "phosphorylating", "STAT5A", ",", "whereas", "recombinant", "BTK", "proteins", "with", "SH1/kinase", "domain", "mutations", "were", "not", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 3, 0], "tokens": ["In", "pull-down", "experiments", ",", "only", "full-length", "BTK", "and", "its", "SH1/kinase", "domain", "(", "but", "not", "the", "pleckstrin", "homology", ",", "SH2", ",", "or", "SH3", "domains", ")", "were", "capable", "of", "binding", "STAT5A", "."]} {"tags": [0, 0, 3, 4, 4, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ectopically", "expressed", "BTK", "kinase", "domain", "was", "capable", "of", "tyrosine-phosphorylating", "STAT5A", "both", "in", "vitro", "and", "in", "vivo", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["BTK", "-mediated", "tyrosine", "phosphorylation", "of", "ectopically", "expressed", "wild", "type", "(", "but", "not", "Tyr", "(", "694", ")", "mutant", ")", "STAT5A", "enhanced", "its", "DNA", "binding", "activity", "."]} {"tags": [0, 3, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "BTK", "-competent", "chicken", "B", "cells", ",", "anti-IgM-stimulated", "tyrosine", "phosphorylation", "of", "STAT5", "protein", "was", "prevented", "by", "pretreatment", "with", "the", "BTK", "inhibitor", "LFM-A13", "but", "not", "by", "pretreatment", "with", "the", "JAK3", "inhibitor", "HI-P131", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 5, 6, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 0, 0, 5, 6, 0, 0, 3, 4, 4, 0], "tokens": ["B", "cell", "antigen", "receptor", "ligation", "resulted", "in", "enhanced", "tyrosine", "phosphorylation", "of", "STAT5", "in", "BTK", "-deficient", "chicken", "B", "cells", "reconstituted", "with", "wild", "type", "human", "BTK", "but", "not", "in", "BTK", "-deficient", "chicken", "B", "cells", "reconstituted", "with", "kinase-inactive", "mutant", "BTK", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Similarly", ",", "anti-IgM", "stimulation", "resulted", "in", "enhanced", "tyrosine", "phosphorylation", "of", "STAT5A", "in", "BTK", "-competent", "B", "cells", "from", "wild", "type", "mice", "but", "not", "in", "BTK", "-deficient", "B", "cells", "from", "XID", "mice", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", "to", "B", "cells", "from", "XID", "mice", ",", "B", "cells", "from", "JAK3", "knockout", "mice", "showed", "a", "normal", "STAT5A", "phosphorylation", "response", "to", "anti-IgM", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6], "tokens": ["These", "findings", "provide", "unprecedented", "experimental", "evidence", "that", "BTK", "plays", "a", "nonredundant", "and", "pivotal", "role", "in", "B", "cell", "antigen", "receptor-mediated", "STAT5A", "activation", "in", "B", "cells"]} {"tags": [0, 0, 3, 0, 0, 0, 5, 6, 0, 3, 0, 0, 0], "tokens": ["Role", "of", "T-bet", "in", "commitment", "of", "TH1", "cells", "before", "IL-12", "-dependent", "selection", "."]} {"tags": [0, 3, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0], "tokens": ["How", "cytokines", "control", "differentiation", "of", "helper", "T", "(", "TH", ")", "cells", "is", "controversial", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 1, 2, 2, 0, 0], "tokens": ["We", "show", "that", "T-bet", ",", "without", "apparent", "assistance", "from", "interleukin", "12", "(", "IL-12", ")", "/", "STAT4", ",", "specifies", "TH1", "effector", "fate", "by", "targeting", "chromatin", "remodeling", "to", "individual", "interferon-gamma", "(", "IFN-gamma", ")", "alleles", "and", "by", "inducing", "IL-12", "receptor", "beta2", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0], "tokens": ["Subsequently", ",", "it", "appears", "that", "IL-12", "/", "STAT4", "serves", "two", "essential", "functions", "in", "the", "development", "of", "TH1", "cells", ":", "as", "growth", "signal", ",", "inducing", "survival", "and", "cell", "division", ";", "and", "as", "trans-activator", ",", "prolonging", "IFN-gamma", "synthesis", "through", "a", "genetic", "interaction", "with", "the", "coactivator", ",", "CREB-binding", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "suggest", "that", "a", "cytokine", "does", "not", "simply", "induce", "TH", "fate", "choice", "but", "instead", "may", "act", "as", "an", "essential", "secondary", "stimulus", "that", "mediates", "selective", "survival", "of", "a", "lineage", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Stat6", "is", "necessary", "and", "sufficient", "for", "IL-4", "'s", "role", "in", "Th2", "differentiation", "and", "cell", "expansion", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["IL-4", "plays", "a", "critical", "role", "in", "the", "differentiation", "of", "T", "CR-stimulated", "naive", "CD4", "T", "cells", "to", "the", "Th2", "phenotype", "."]} {"tags": [0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0, 0, 3, 0], "tokens": ["In", "response", "to", "IL-4", ",", "the", "IL-4R", "activates", "a", "set", "of", "phosphotyrosine", "binding", "domain-containing", "proteins", ",", "including", "insulin", "receptor", "substrate", "1/2", ",", "Shc", ",", "and", "IL-4R", "interacting", "protein", ",", "as", "well", "as", "Stat6", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Stat6", "has", "been", "shown", "to", "be", "required", "for", "Th2", "differentiation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "determine", "the", "roles", "of", "the", "phosphotyrosine", "binding", "adaptors", "in", "Th2", "differentiation", ",", "we", "prepared", "a", "retrovirus", "containing", "a", "mutant", "of", "the", "human", "(", "h", ")", "IL-4R", "alpha-chain", ",", "Y497F", ",", "which", "is", "unable", "to", "recruit", "these", "adaptors", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["The", "mutant", "hIL-4Ralpha", ",", "as", "well", "as", "the", "wild-type", "(", "WT", ")", "hIL-4Ralpha", ",", "was", "introduced", "into", "naive", "CD4", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Upon", "hIL-4", "stimulation", ",", "Y497F", "worked", "as", "well", "as", "the", "WT", "hIL-4Ralpha", "in", "driving", "Th2", "differentiation", ",", "as", "measured", "by", "Gata3", "up-regulation", "and", "IL-4", "production", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Furthermore", ",", "IL-4-driven", "cell", "expansion", "was", "also", "normal", "in", "the", "cells", "infected", "with", "Y497F", ",", "although", "cells", "infected", "with", "Y497F", "were", "not", "capable", "of", "phosphorylating", "insulin", "receptor", "substrate", "2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "suggest", "that", "the", "signal", "pathway", "mediated", "by", "Y497", "is", "dispensable", "for", "both", "IL-4", "-driven", "Th2", "differentiation", "and", "cell", "expansion", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Both", "WT", "and", "Y497F", "hIL-4Ralpha", "lose", "the", "ability", "to", "drive", "Th2", "differentiation", "and", "cell", "expansion", "in", "Stat6-knockout", "CD4", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "constitutively", "activated", "form", "of", "Stat6", "introduced", "into", "CD4", "T", "cells", "resulted", "in", "both", "Th2", "differentiation", "and", "enhanced", "cell", "expansion", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Thus", ",", "activated", "Stat6", "is", "necessary", "and", "sufficient", "to", "mediate", "both", "IL-4", "-driven", "Th2", "differentiation", "and", "cell", "expansion", "in", "CD4", "T", "cells", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 3, 0], "tokens": ["The", "effect", "of", "HIV-1", "regulatory", "proteins", "on", "cellular", "genes", ":", "derepression", "of", "the", "IL-2", "promoter", "by", "Tat", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "HIV-infected", "individuals", "dysregulation", "of", "the", "immune", "system", "is", "characterized", "by", "severe", "disorders", "of", "the", "cytokine", "network", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0], "tokens": ["Increase", "secretion", "of", "IL-2", ",", "the", "major", "T", "cell", "growth", "and", "differentiation", "cytokine", ",", "may", "play", "a", "decisive", "role", "in", "sensitization", "of", "T", "cells", "for", "activation", "induced", "apoptosis", "and", "indirect", "death", "of", "activated", "T", "cells", "through", "augmented", "virus", "replication", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["We", "investigated", "the", "cause", "of", "enhanced", "IL-2", "secretion", "and", "found", "that", "the", "HIV", "Tat", "induces", "this", "effect", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 1, 2, 0, 0], "tokens": ["We", "demonstrate", "that", "increased", "IL-2", "secretion", "is", "due", "to", "Tat", "-enhanced", "IL-2", "promoter", "activation", "."]} {"tags": [3, 0, 0, 0, 0, 1, 2, 2, 0, 1, 2, 2, 2, 0, 0, 0, 1, 2, 0], "tokens": ["Tat", "derepresses", "and", "activates", "the", "distal", "AP-1", "site", "(", "position", "-185", "to", "-177", ")", "in", "the", "IL-2", "promoter", "."]} {"tags": [0, 5, 6, 6, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 1, 2, 0, 0], "tokens": ["In", "nonstimulated", "T", "cells", "a", "repressor", "complex", "containing", "NF-IL6", ",", "JunB", ",", "c-Fos", "and", "Fra-1", "is", "formed", "on", "the", "AP-1", "(", "IL-2/d", ")", "site", "and", "represses", "IL-2", "promoter", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 3, 0, 0, 0, 1, 2, 2, 2, 2, 0], "tokens": ["After", "T", "cell", "activation", ",", "a", "heterodimeric", "activator", "containing", "p65", "and", "c-Jun", "binds", "to", "the", "AP-1", "(", "IL-2/d", ")", "site", "."]} {"tags": [3, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0], "tokens": ["HIV", "Tat", "enhances", "activation", "of", "NF-kappaB", "and", "consequently", ",", "activates", "the", "AP-1", "(", "IL-2/d", ")", "site", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "data", "provide", "evidence", "for", "a", "novel", "mechanism", "by", "which", "HIV", "Tat", "dysregulates", "IL-2", "production", "and", "therefore", "may", "contribute", "to", "the", "HIV-1", "infection", "in", "a", "way", "yet", "to", "be", "clarified", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Transforming", "growth", "factor-beta1", "interferes", "with", "thrombopoietin-induced", "signal", "transduction", "in", "megakaryoblastic", "and", "erythroleukemic", "cells", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["OBJECTIVE", ":", "Thrombopoietin", "(", "TPO", ")", "and", "transforming", "growth", "factor-beta", "(", "1", ")", "(", "TGF-beta", "(", "1", ")", ")", "have", "been", "shown", "to", "exert", "opposite", "effects", "on", "proliferation", "and", "megakaryocytic", "differentiation", "of", "hematopoietic", "cells", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "determine", "whether", "TGF-beta", "(", "1", ")", "interferes", "directly", "with", "TPO", "-induced", "signal", "transduction", "in", "hematopoietic", "cells", ",", "we", "compared", "the", "regulatory", "effects", "in", "the", "TPO", "-responsive", "cell", "lines", "Mo-7e", "and", "HEL", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["MATERIALS", "AND", "METHODS", ":", "The", "cells", "were", "stimulated", "by", "100", "ng/mL", "TPO", "and/or", "100", "ng/mL", "TGF-beta1", "and", "analyzed", "for", "proliferation", "(", "3H", "thymidine", "incorporation", ")", ",", "viability", "(", "trypan", "blue", "exclusion", ")", ",", "and", "protein", "expression", "and", "phosphorylation", "(", "Western", "blot", ")", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["RESULTS", ":", "TPO", "enhanced", "the", "proliferation", "of", "Mo-7e", "cells", "as", "determined", "by", "3H-thymidine", "incorporation", ",", "whereas", "TGF-beta1", "suppressed", "baseline", "cell", "growth", "and", "antagonized", "the", "proliferative", "effect", "of", "TPO", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["TPO", "-induced", "proliferation", "also", "was", "reduced", "by", "a", "specific", "inhibitor", "of", "the", "mitogen-activated", "protein", "kinase", "(", "MAPK", ")", "pathway", "(", "PD098059", ")", ",", "which", "inhibits", "activation", "of", "the", "MAPK", "extracellular", "signal-regulated", "kinases", "(", "ERK", ")", "ERK1", "and", "ERK2", ",", "and", "AG490", ",", "an", "inhibitor", "of", "Janus", "kinase-2", ",", "which", "completely", "blocked", "TPO", "-induced", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 7, 8, 8, 8, 0], "tokens": ["As", "demonstrated", "by", "Western", "blotting", ",", "TGF-beta1", "reduced", "the", "TPO", "-stimulated", "ERK1", "/", "ERK2", "and", "STAT5", "phosphorylation", "in", "Mo-7e", "and", "HEL", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0], "tokens": ["This", "effect", "was", "completely", "reversed", "by", "preincubation", "with", "a", "tyrosine", "phosphatase", "inhibitor", "(", "Na3VO4", ")", ",", "which", "suggests", "that", "TGF-beta1", "activated", "a", "phosphatase", "."]} {"tags": [0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 0], "tokens": ["Although", "STAT3", "also", "was", "activated", "by", "TPO", ",", "STAT3", "activation", "remained", "unaltered", "by", "TGF-beta1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["CONCLUSION", ":", "Taken", "together", ",", "these", "data", "suggest", "that", "TGF-beta1", "modulates", "TPO", "-mediated", "effects", "on", "megakaryocytic", "proliferation", "by", "interfering", "with", "TPO", "-induced", "signal", "transduction", ",", "particularly", "by", "reducing", "the", "activities", "of", "MAPK", "ERK1/ERK2", "and", "STAT5", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0], "tokens": ["Invariant", "chain", "induces", "B", "cell", "maturation", "by", "activating", "a", "TAF", "(", "II", ")", "105-NF-kappaB", "-dependent", "transcription", "program", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Early", "stages", "of", "B", "cell", "development", "occur", "in", "the", "bone", "marrow", ",", "resulting", "in", "formation", "of", "immature", "B", "cells", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["From", "there", "these", "immature", "cells", "migrate", "to", "the", "spleen", "where", "they", "differentiate", "to", "mature", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "final", "maturation", "step", "is", "crucial", "for", "the", "B", "cells", "to", "become", "responsive", "to", "antigens", "and", "to", "participate", "in", "the", "immune", "response", "."]} {"tags": [0, 0, 3, 4, 0, 3, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 3, 4, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Recently", ",", "invariant", "chain", "(", "Ii", ")", ",", "a", "major", "histocompatibility", "complex", "class", "II", "chaperone", ",", "as", "well", "as", "the", "transcription", "factors", "c-Rel", "and", "p65/RelA", ",", "were", "found", "to", "play", "a", "role", "in", "the", "final", "antigen-independent", "differentiation", "stage", "of", "B", "cells", "in", "the", "spleen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", ",", "we", "investigated", "a", "possible", "link", "between", "Ii", "-dependent", "B", "cell", "maturation", "and", "the", "NF-kappaB", "pathway", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 3, 4, 4, 3, 4, 4, 4, 4, 4, 0], "tokens": ["Our", "studies", "indicate", "that", "Ii", "-induced", "B", "cell", "maturation", "involves", "activation", "of", "transcription", "mediated", "by", "the", "NF-kappaB", "p65/RelA", "homodimer", "and", "requires", "the", "B", "cell-enriched", "coactivator", "TBP-associated", "factor", "(", "II", ")", "105", "."]} {"tags": [0, 0, 0, 5, 0, 0], "tokens": ["Androgens", "indirectly", "accelerate", "thymocyte", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Apoptotic", "processes", ",", "or", "the", "disturbance", "of", "the", "natural", "regulation", "of", "these", "processes", ",", "may", "be", "involved", "in", "the", "pathogenesis", "of", "autoimmune", "diseases", "(", "AID", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Women", "are", ",", "in", "general", ",", "more", "susceptible", "than", "men", "to", "develop", "AID", "like", "rheumatoid", "arthritis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Androgens", "and", "glucocorticoids", ",", "in", "contrast", "to", "oestrogens", ",", "have", "favourable", "effects", "in", "AID", "models", "as", "well", "as", "in", "human", "AID", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["It", "is", "known", "that", "glucocorticoids", "(", "GC", ")", ",", "used", "for", "treatment", "of", "AID", ",", "increase", "apoptosis", "in", "the", "thymus", "resulting", "in", "decreased", "numbers", "of", "CD4+", "CD8+", "thymocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["It", "was", "asked", "whether", "androgens", ",", "in", "contrast", "to", "oestrogens", ",", "exert", "their", "favourable", "effects", "in", "the", "treatment", "of", "AID", "by", "a", "mechanism", "comparable", "to", "that", "described", "for", "GC", "by", "eliminating", "the", "apoptosis", "prone", "CD4+", "CD8+", "population", "in", "the", "thymus", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "both", "androgens", "and", "oestrogens", "proved", "thymolytic", ",", "a", "significantly", "decreased", "percentage", "of", "CD4+", "CD8+", "thymocytes", "was", "observed", "by", "flow", "cytometry", "after", "treatment", "of", "mice", "with", "the", "androgen", "methyltestosterone", ",", "but", "not", "with", "the", "oestrogen", "ethinylestradiol", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "investigate", "whether", "the", "observed", "thymolytic", "effects", "were", "due", "to", "the", "presence", "of", "hormone", "receptors", "on", "thymocytes", ",", "cells", "were", "isolated", "from", "the", "thymus", "and", "incubated", "with", "androgens", "or", "oestrogens", "to", "measure", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Several", "techniques", "were", "used", "to", "determine", "thymocyte", "apoptosis", "in", "vitro", ",", "but", "no", "enhanced", "apoptotic", "signal", "was", "observed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "the", "very", "sensitive", "TUNEL", "assay", ",", "no", "direct", "effect", "of", "androgens", "on", "thymocytes", "in", "vitro", "could", "be", "observed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "is", "in", "sharp", "contrast", "to", "the", "high", "signal", "observed", "with", "GC", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", ",", "upon", "in", "vivo", "androgen", "treatment", ",", "other", "cells", "containing", "androgen", "receptors", "than", "thymocytes", "are", "probably", "involved", "in", "inducing", "the", "increase", "in", "thymic", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "study", "the", "role", "of", "the", "androgen", "receptor", "on", "thymocyte", "apoptosis", ",", "androgen", "receptor", "mutant", "(", "Tfm/Y", ")", "mice", "were", "treated", "with", "androgens", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["No", "alterations", "of", "thymocyte", "subpopulations", "were", "seen", ",", "suggesting", "that", "changes", "in", "the", "percentage", "of", "CD4+", "CD8+", "thymocytes", "after", "administration", "of", "androgens", "depend", "on", "the", "presence", "of", "functional", "androgen", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["Thus", ",", "it", "is", "concluded", "that", "androgens", "indirectly", "accelerate", "thymocyte", "apoptosis", "in", "vivo", "."]} {"tags": [0, 0, 0, 3, 4, 3, 0, 3, 0, 5, 6, 6, 0, 3, 4, 4, 4, 4, 0], "tokens": ["Inhibition", "of", "the", "transcription", "factors", "AP-1", "and", "NF-kappaB", "in", "CD4", "T", "cells", "by", "peroxisome", "proliferator-activated", "receptor", "gamma", "ligands", "."]} {"tags": [0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "peroxisome", "proliferator-activated", "receptor", "gamma", "(", "PPARgamma", ")", ",", "a", "member", "of", "the", "nuclear", "hormone", "receptor", "superfamily", ",", "is", "essential", "for", "adipocyte", "differentiation", "and", "glucose", "homeostasis", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["PPARgamma", "has", "been", "found", "recently", "to", "regulate", "macrophage", "activation", "in", "response", "to", "mitogens", "and", "inflammation", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "study", "shows", "PPARgamma", "to", "be", "preferentially", "expressed", "in", "the", "nuclei", "of", "resting", "T", "cells", "and", "to", "increase", "upon", "activation", "of", "T", "cells", "by", "either", "anti-CD3", "and", "anti-CD28", "or", "phorbol", "myristyl", "acetate", "(", "PMA", ")", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0], "tokens": ["We", "also", "found", "the", "PPARgamma", "ligand", "ciglitizone", "to", "attenuate", "the", "activation", "of", "T", "cells", "by", "inhibiting", "cytokine", "gene", "expression", "and", "anti-CD3", "and", "anti-CD28", "or", "PMA-induced", "proliferative", "responses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 0, 3, 0], "tokens": ["Inhibition", "of", "both", "the", "proliferative", "response", "and", "inflammatory", "cytokine", "expression", "in", "CD4", "T", "cells", "was", "correlated", "with", "suppression", "of", "the", "activated", "transcription", "factors", "AP1", "and", "NF-kappaB", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["PPARgamma", "ligands", "also", "strongly", "inhibited", "SEA-induced", "Vbeta3", "T", "cell", "activation", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 0, 5, 6, 0], "tokens": ["These", "results", ",", "together", "with", "previous", "findings", "of", "the", "inhibitory", "effect", "of", "PPARgamma", "ligands", "on", "activated", "macrophages", ",", "provide", "clear", "evidence", "for", "PPARgamma", "as", "a", "negative", "regulator", "of", "the", "inflammatory", "activation", "of", "both", "macrophage", "and", "T", "cells", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["PPARgamma", "may", "thus", "be", "a", "potential", "therapeutic", "target", "for", "the", "treatment", "of", "autoimmunity", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 5, 6, 0], "tokens": ["A", "prominent", "role", "for", "activator", "protein-1", "in", "the", "transcription", "of", "the", "human", "2B4", "(", "CD244", ")", "gene", "in", "NK", "cells", "."]} {"tags": [0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 0, 5, 6, 0, 0], "tokens": ["The", "cell", "surface", "glycoprotein", "2B4", "(", "CD244", ")", "of", "the", "Ig", "superfamily", "is", "involved", "in", "the", "regulation", "of", "NK", "and", "T", "lymphocyte", "functions", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "recently", "identified", "CD48", "as", "the", "high", "affinity", "counterreceptor", "for", "2B4", "in", "both", "mice", "and", "humans", "."]} {"tags": [0, 3, 4, 0, 3, 0, 0, 3, 4, 4, 4, 4, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "cytoplasmic", "domain", "of", "2B4", "associates", "with", "src", "homology", "2", "domain-containing", "protein", "or", "signaling", "lymphocyte", "activation", "molecule-associated", "protein", ",", "whose", "mutation", "is", "the", "underlying", "genetic", "defect", "in", "the", "X-linked", "lymphoproliferative", "syndrome", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0], "tokens": ["In", "this", "study", ",", "we", "report", "the", "molecular", "cloning", "and", "characterization", "of", "the", "human", "2B4", "(", "h2B4", ")", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0], "tokens": ["Through", "primer", "extension", "analysis", ",", "we", "found", "that", "the", "transcription", "of", "the", "h2B4", "gene", "initiates", "at", "multiple", "start", "sites", "."]} {"tags": [0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 1, 2, 0], "tokens": ["We", "isolated", "h2B4", "genomic", "clones", "and", "PCR", "amplified", "the", "5", "'", "untranslated", "region", "containing", "the", "promoter", "elements", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 7, 8, 8, 8, 0], "tokens": ["We", "have", "identified", "a", "functional", "AP-1", "site", "that", "lies", "between", "(", "-106", "to", "-100", ")", "through", "transient", "transfection", "analysis", "in", "YT", "cells", ",", "a", "human", "NK", "cell", "line", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["EMSAs", "with", "Abs", "specific", "for", "various", "protein", "factors", "of", "the", "AP-1", "family", "revealed", "that", "multiple", "members", "of", "the", "Jun", "family", "are", "involved", "in", "the", "regulation", "of", "the", "h2B4", "gene", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mutation", "of", "the", "AP-1", "site", "not", "only", "abolishes", "protein/DNA", "interactions", "but", "also", "promoter", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["These", "results", "demonstrate", "a", "significant", "role", "for", "AP-1", "in", "the", "transcriptional", "regulation", "of", "the", "h2B4", "gene", "."]} {"tags": [0, 0, 0, 5, 6, 0, 3, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Functional", "correction", "of", "FA-C", "cells", "with", "FANCC", "suppresses", "the", "expression", "of", "interferon", "gamma-inducible", "genes", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Because", "hematopoietic", "cells", "derived", "from", "Fanconi", "anemia", "(", "FA", ")", "patients", "of", "the", "C-complementation", "group", "(", "FA-C", ")", "are", "hypersensitive", "to", "the", "inhibitory", "effects", "of", "interferon", "gamma", "(", "IFNgamma", ")", ",", "the", "products", "of", "certain", "IFNgamma-inducible", "genes", "known", "to", "influence", "hematopoietic", "cell", "survival", "were", "quantified", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 3, 0, 5, 6, 0, 5, 6, 6, 6, 0, 0, 5, 6, 6, 0], "tokens": ["High", "constitutive", "expression", "of", "the", "IFNgamma-inducible", "genes", ",", "IFN-stimulated", "gene", "factor", "3", "gamma", "subunit", "(", "ISGF3gamma", ")", ",", "IFN", "regulatory", "factor-1", "(", "IRF-1", ")", ",", "and", "the", "cyclin-dependent", "kinase", "inhibitor", "p21", "(", "WAF1", ")", "was", "found", "in", "FANCC", "mutant", "B", "lymphoblasts", ",", "low-density", "bone", "marrow", "cells", ",", "and", "murine", "embryonic", "fibroblasts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0], "tokens": ["Paradoxically", ",", "these", "cells", "do", "not", "activate", "signal", "transducer", "and", "activator", "of", "transcription", "(", "STAT", ")", "1", "properly", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["In", "an", "attempt", "to", "clarify", "mechanisms", "by", "which", "FA-C", "cells", "overexpress", "IFNgamma-inducible", "genes", "in", "the", "face", "of", "defective", "STAT1", "phosphorylation", ",", "it", "was", "reasoned", "that", "decreased", "levels", "of", "activated", "STAT1", "might", "result", "in", "reduced", "expression", "of", "a", "hematopoietic", "IFNgamma-responsive", "protein", "that", "normally", "modulates", "expression", "of", "other", "IFNgamma-responsive", "genes", "."]} {"tags": [0, 0, 0, 3, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 3, 4, 4, 0, 0, 1, 2, 0, 0, 0, 0], "tokens": ["Levels", "of", "the", "IFNgamma", "-inducible", "factor", "IFN", "consensus", "sequence", "binding", "protein", "(", "ICSBP", ")", ",", "a", "negative", "trans-acting", "regulator", "of", "some", "IFNgamma-inducible", "genes", ",", "were", "quantified", "."]} {"tags": [3, 0, 0, 0, 0, 5, 6, 6, 0, 5, 0], "tokens": ["ICSBP", "levels", "were", "reduced", "in", "FA-C", "B", "lymphoblasts", "and", "MEFs", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 3, 0, 0, 3, 4, 4, 4, 0], "tokens": ["However", ",", "enforced", "expression", "of", "ICSBP", "failed", "to", "down-regulate", "IRF-1", ",", "ISGF3gamma", ",", "and", "p21", "(", "WAF1", ")", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Thus", ",", "the", "FANCC", "protein", "functions", "to", "modulate", "expression", "of", "a", "family", "of", "genes", "that", "in", "normal", "cells", "are", "inducible", "only", "by", "specific", "environmental", "cues", "for", "apoptosis", "or", "mitogenic", "inhibition", ",", "but", "it", "does", "so", "independently", "of", "the", "classic", "IFN-STAT1", "pathway", "and", "is", "not", "the", "direct", "result", "of", "reduced", "ICSBP", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "genetic", "investigation", "of", "E2A", "function", "in", "lymphocyte", "development", "."]} {"tags": [5, 0, 0, 0, 5, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymphocytes", "are", "derived", "from", "hematopoietic", "stem", "cells", "(", "HSC", ")", "following", "a", "series", "of", "regulated", "differentiation", "events", "."]} {"tags": [5, 6, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Multipotent", "HSCs", "become", "committed", "to", "the", "B", "cell", "lineage", "in", "bone", "marrow", "and", "the", "T", "cell", "lineage", "in", "the", "thymus", "after", "receiving", "appropriate", "signals", "from", "the", "corresponding", "microenvironment", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0], "tokens": ["These", "committed", "lymphoid", "cells", "must", "then", "undergo", "V", "(", "D", ")", "J", "recombination", "at", "the", "immunoglobulin", "gene", "or", "T", "cell", "receptor", "gene", "locus", "resulting", "in", "clonal", "production", "of", "functional", "B", "or", "T", "lymphocytes", ",", "respectively", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0], "tokens": ["Lymphocyte", "commitment", "and", "differentiation", "are", "accompanied", "by", "programmed", "gene", "expression", "or", "repression", "events", "which", "are", "driven", "by", "lineage", "and", "stage", "specific", "transcription", "factors", "."]} {"tags": [0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "basic-helix-loop-helix", "(", "bHLH", ")", "transcription", "factors", "encoded", "by", "the", "E2A", "gene", "are", "involved", "in", "several", "differentiation", "events", "during", "B", "and", "T", "cell", "development", ",", "including", "lineage", "commitment", ",", "initiation", "of", "V", "(", "D", ")", "J", "recombination", ",", "and", "antigen", "receptor", "mediated", "proliferation", "and", "differentiation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Several", "recent", "reviews", "have", "provided", "a", "comprehensive", "discussion", "of", "biochemical", ",", "cellular", ",", "and", "genetic", "research", "on", "E2A", "function", "in", "lymphocyte", "development", "(", "1", ",", "2", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0], "tokens": ["Here", ",", "we", "only", "discuss", "some", "of", "the", "genetic", "approaches", "our", "laboratory", "(", "except", "where", "it", "is", "noted", ")", "has", "undertaken", "to", "investigate", "the", "molecular", "pathways", "mediated", "by", "E2A", "transcription", "factors", "in", "lymphocyte", "development"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["D609", "inhibits", "ionizing", "radiation-induced", "oxidative", "damage", "by", "acting", "as", "a", "potent", "antioxidant", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tricyclodecan-9-yl-xanthogenate", "(", "D609", ")", "has", "been", "extensively", "studied", "in", "biological", "systems", "and", "exhibits", "a", "variety", "of", "biological", "functions", ",", "including", "antiviral", ",", "antitumor", ",", "and", "anti-inflammatory", "activities", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Most", "of", "these", "activities", "have", "been", "largely", "attributed", "to", "the", "inhibitory", "effect", "of", "D609", "on", "phosphatidylcholine-specific", "phospholipase", "C", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "as", "a", "xanthate", "derivative", ",", "D609", "is", "a", "strong", "electrolyte", "and", "readily", "dissociates", "to", "xanthate", "anions", "and", "cations", "of", "alkali", "metals", "in", "solution", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Xanthate", "anions", "and", "protonated", "xanthic", "acid", "contain", "a", "free", "thiol", "moiety", "and", "are", "highly", "reductive", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "implies", "that", "D609", "and", "other", "xanthate", "derivatives", "may", "function", "as", "potent", "antioxidants", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Indeed", ",", "we", "found", "that", "D609", "inhibited", "the", "Fenton", "reaction-induced", "oxidation", "of", "dihydrorhodamine", "123", "in", "a", "dose-dependent", "manner", "similar", "to", "that", "of", "pyrrolidinedithiocarbamate", ",", "a", "well", "known", "antioxidant", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "D609", "inhibited", "the", "formation", "of", "the", "alpha-phenyl-tert-butylnitrone-free", "radical", "spin", "adducts", "and", "lipid", "peroxidation", "of", "synaptosomal", "membranes", "by", "the", "Fenton", "reagents", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Furthermore", ",", "preincubation", "of", "lymphocytes", "with", "D609", "resulted", "in", "a", "significant", "diminution", "of", "ionizing", "radiation", "(", "IR", ")", "-induced", "1", ")", "production", "of", "reactive", "oxygen", "species", ";", "2", ")", "decrease", "in", "intracellular", "reduced", "glutathione", ";", "3", ")", "oxidative", "damage", "to", "proteins", "and", "lipids", ";", "and", "4", ")", "activation", "of", "nuclear", "factor-kappaB", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "when", "D609", "(", "50", "mg/kg", "i.v.", ")", "was", "administered", "to", "mice", "10", "min", "prior", "to", "total", "body", "IR", "(", "6.5", "and", "8.5", "Gy", ")", ",", "it", "protected", "the", "mice", "from", "IR-induced", "lethality", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "these", "results", "indicate", "that", "D609", "is", "a", "potent", "antioxidant", "and", "has", "the", "ability", "to", "inhibit", "IR-induced", "cellular", "oxidative", "stress", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 4, 0], "tokens": ["Inhibition", "of", "AP-1", "by", "the", "glucocorticoid-inducible", "protein", "GILZ", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "immunosuppressive", "effects", "of", "glucocorticoids", "arise", "largely", "by", "inhibition", "of", "cytokine", "gene", "expression", ",", "which", "has", "been", "ascribed", "to", "interference", "between", "the", "glucocorticoid", "receptor", "and", "transcription", "factors", "such", "as", "AP-1", "and", "NF-kappa", "B", "as", "well", "as", "by", "competition", "for", "common", "coactivators", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0], "tokens": ["Here", "we", "show", "that", "glucocorticoid-induced", "inhibition", "of", "interleukin-2", "mRNA", "expression", "in", "activated", "normal", "T", "cells", "required", "new", "protein", "synthesis", ",", "suggesting", "that", "this", "phenomenon", "is", "secondary", "to", "expression", "of", "glucocorticoid-regulated", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 0], "tokens": ["One", "of", "the", "most", "prominent", "glucocorticoid-induced", "genes", "is", "glucocorticoid-induced", "leucine", "zipper", "(", "GILZ", ")", ",", "which", "has", "been", "reported", "to", "inhibit", "activation-induced", "up-regulation", "of", "Fas", "ligand", "(", "FasL", ")", "mRNA", "."]} {"tags": [0, 0, 0, 0, 0, 1, 0, 7, 8, 8, 0, 0, 0, 0, 1, 2, 0, 0, 0, 1, 2, 0], "tokens": ["Indeed", ",", "transient", "expression", "of", "GILZ", "in", "Jurkat", "T", "cells", "blocked", "induction", "of", "a", "reporter", "construct", "driven", "by", "the", "FasL", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 3, 0, 3, 0, 3, 4, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0], "tokens": ["This", "could", "be", "accounted", "for", "by", "GILZ", "-mediated", "inhibition", "of", "Egr-2", "and", "Egr-3", ",", "NFAT/AP-1", "-inducible", "transcription", "factors", "that", "bind", "a", "regulatory", "element", "in", "the", "FasL", "promoter", "and", "up-regulate", "FasL", "expression", "."]} {"tags": [3, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 3, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["GILZ", "also", "potently", "inhibited", "AP-1-driven", "and", "IL-2", "promoter-driven", "reporter", "constructs", ",", "and", "recombinant", "GILZ", "specifically", "interacted", "with", "c-Fos", "and", "c-Jun", "in", "vitro", "and", "inhibited", "the", "binding", "of", "active", "AP-1", "to", "its", "target", "DNA", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 3, 4, 4, 0, 3, 0], "tokens": ["Whereas", "homodimerization", "of", "GILZ", "required", "the", "presence", "of", "its", "leucine", "zipper", ",", "the", "interaction", "with", "c-Fos", "and", "c-Jun", "occurred", "through", "the", "N-terminal", "60-amino", "acid", "region", "of", "GILZ", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "GILZ", "represents", "a", "glucocorticoid-induced", "gene", "product", "that", "can", "inhibit", "a", "variety", "of", "activation-induced", "events", ",", "at", "least", "in", "part", "by", "direct", "interference", "with", "AP-1", ",", "and", "is", "therefore", "a", "candidate", "for", "a", "mediator", "of", "glucocorticoid-induced", "immunosuppression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Pharmacokinetic", "differences", "between", "a", "T", "cell-tolerizing", "and", "a", "T", "cell-activating", "peptide", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Vaccination", "with", "a", "peptide", "representing", "a", "CTL", "epitope", "from", "the", "human", "papillomavirus", "(", "HPV", ")", "16", "E7", "protein", "induces", "a", "specific", "CTL", "response", "that", "prevents", "the", "outgrowth", "of", "HPV16", "E7-expressing", "tumors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "vaccination", "with", "a", "peptide", "encoding", "an", "adenovirus", "type", "5", "(", "Ad5", ")", "E1A", "CTL", "epitope", "results", "in", "CTL", "tolerance", "and", "enhanced", "growth", "of", "an", "Ad5", "E1A-expressing", "tumor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "is", "unclear", "why", "these", "peptides", "induce", "such", "opposite", "effects", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "determine", "whether", "a", "difference", "in", "pharmacokinetics", "can", "explain", "the", "functional", "contrasts", ",", "tritiated", "Ad5", "E1A", "and", "HPV16", "E7", "peptides", "were", "injected", "into", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Results", "show", "that", "the", "tolerizing", "peptide", "spread", "through", "the", "body", "16", "times", "faster", "than", "the", "activating", "peptide", "and", "was", "cleared", "at", "least", "2", "times", "faster", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["The", "HPV16", "E7", "peptide", "kinetics", "correlated", "with", "the", "kinetics", "of", "HPV16", "E7", "-specific", "CTL", "induction", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "Ad5", "E1A", "peptide", "injection", "resulted", "in", "physical", "deletion", "of", "preexisting", "Ad5", "E1A", "-specific", "CTLs", "within", "24", "h", "after", "injection", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "tolerization", "occurred", "at", "the", "time", "when", "the", "peptide", "reached", "its", "maximum", "peptide", "concentration", "in", "the", "organs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0], "tokens": ["These", "data", "suggest", "that", "ubiquitous", "expression", "of", "the", "tolerizing", "Ad5", "E1A", "peptide", "within", "a", "short", "period", "of", "time", "causes", "activation-induced", "cell", "death", "of", "Ad5", "E1A", "-specific", "CTLs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", ",", "information", "on", "the", "pharmacokinetics", "of", "peptides", "is", "vital", "for", "the", "safety", "and", "efficacy", "of", "peptide-based", "vaccines", "."]} {"tags": [3, 0, 3, 0, 3, 4, 4, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Smad3", "and", "Smad4", "mediate", "transforming", "growth", "factor-beta1", "-induced", "IgA", "expression", "in", "murine", "B", "lymphocytes", "."]} {"tags": [3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 3, 0, 1, 0], "tokens": ["Transforming", "growth", "factor", "(", "TGF", ")", "-beta1", "is", "well", "established", "as", "a", "critical", "IgA", "isotype", "switching", "factor", "and", "Smad", "molecules", "have", "been", "reported", "to", "act", "as", "transducers", "and", "transcriptional", "factors", "in", "the", "expression", "of", "TGF-beta1", "-targeted", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0], "tokens": ["We", "examined", "the", "involvement", "of", "Smad", "proteins", "in", "TGF-beta1", "-induced", "IgA", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 9, 10, 10, 10, 10, 10, 10, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 8, 0], "tokens": ["First", ",", "we", "found", "that", "TGF-beta1", "significantly", "increases", "endogenous", "germ-line", "(", "GL", ")", "alpha", "transcripts", "by", "LPS-stimulated", "CH12.LX.4933", "(", "mu", "(", "+", ")", ")", "B", "lymphoma", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 1, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 0, 3, 0], "tokens": ["To", "investigate", "its", "signaling", "mechanisms", ",", "the", "lymphoma", "cell", "line", "was", "transfected", "with", "pFL3", "that", "contains", "the", "TGF-beta-responsive", "element", "of", "the", "GLalpha", "promoter", ",", "and", "stimulated", "with", "TGF-beta1", "."]} {"tags": [0, 0, 9, 10, 10, 0, 3, 0, 1, 2, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Similar", "to", "endogenous", "GLalpha", "transcripts", ",", "TGF-beta1", "induces", "GLalpha", "promoter", "activity", "and", "overexpression", "of", "Smad3", "markedly", "enhances", "the", "promoter", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["This", "activity", "is", "further", "augmented", "by", "cotransfected", "Smad4", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 1, 2, 0, 0], "tokens": ["On", "the", "other", "hand", ",", "Smad7", "substantially", "abrogates", "the", "synergistic", "effect", "of", "Smad3/4", "on", "GLalpha", "promoter", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 9, 10, 10, 0, 5, 6, 6, 6, 0, 0], "tokens": ["In", "addition", ",", "overexpression", "of", "Smad3/4", "enhances", "TGF-beta1", "-induced", "endogenous", "GLalpha", "transcripts", "in", "normal", "spleen", "B", "cell", "s", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Finally", ",", "in", "the", "presence", "of", "TGF-beta1", ",", "overexpression", "of", "Smad3/4", "selectively", "increases", "both", "surface", "IgA", "expression", "and", "IgA", "production", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "results", "from", "the", "present", "study", "indicate", "that", "Smad3", ",", "Smad4", ",", "and", "Smad7", ",", "at", "least", "in", "part", ",", "serve", "as", "mediators", "linking", "TGF-beta1", "to", "transcriptional", "regulation", "of", "IgA", "switching", "related", "gene", "and", "regulation", "of", "IgA", "class", "switching", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "translesion", "DNA", "polymerase", "zeta", "plays", "a", "major", "role", "in", "Ig", "and", "bcl-6", "somatic", "hypermutation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ig", "somatic", "mutations", "would", "be", "introduced", "by", "a", "polymerase", "(", "pol", ")", "while", "repairing", "DNA", "outside", "main", "DNA", "replication", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "show", "that", "human", "B", "cells", "constitutively", "express", "the", "translesion", "pol", "zeta", ",", "which", "effectively", "extends", "DNA", "past", "mismatched", "bases", "(", "mispair", "extender", ")", ",", "and", "pol", "eta", ",", "which", "bypasses", "DNA", "lesions", "in", "an", "error-free", "fashion", "."]} {"tags": [0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 5, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["Upon", "B", "cell", "receptor", "(", "BCR", ")", "engagement", "and", "coculture", "with", "activated", "CD4+", "T", "cells", ",", "these", "lymphocytes", "upregulated", "pol", "zeta", ",", "downregulated", "pol", "eta", ",", "and", "mutated", "the", "Ig", "and", "bcl-6", "genes", "."]} {"tags": [0, 0, 0, 3, 4, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "the", "pol", "zeta", "REV3", "catalytic", "subunit", "by", "specific", "phosphorothioate-modified", "oligonucleotides", "impaired", "Ig", "and", "bcl-6", "hypermutation", "and", "UV", "damage-induced", "DNA", "mutagenesis", ",", "without", "affecting", "cell", "cycle", "or", "viability", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Thus", ",", "pol", "zeta", "plays", "a", "critical", "role", "in", "Ig", "and", "bcl-6", "hypermutation", ",", "perhaps", "facilitated", "by", "the", "downregulation", "of", "pol", "eta", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Molecular", "mechanism", "of", "cell", "cycle", "progression", "induced", "by", "the", "oncogene", "product", "Tax", "of", "human", "T-cell", "leukemia", "virus", "type", "I", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "trans-activator", "protein", "Tax", "of", "human", "T-cell", "leukemia", "virus", "type", "I", "(", "HTLV-I", ")", "plays", "an", "important", "role", "in", "the", "development", "of", "adult", "T-cell", "leukemia", "through", ",", "at", "least", "in", "part", ",", "its", "ability", "to", "stimulate", "cell", "growth", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0], "tokens": ["We", "previously", "reported", "that", "Tax", "induced", "cell", "cycle", "progression", "from", "G0/G1", "phase", "to", "S", "and", "G2/M", "phases", "in", "human", "T-cell", "line", "Kit", "225", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "elucidate", "molecular", "mechanism", "of", "Tax", "-induced", "cell", "cycle", "progression", ",", "we", "systematically", "examined", "the", "effects", "of", "Tax", "on", "biochemical", "events", "associated", "with", "cell", "cycle", "progression", "."]} {"tags": [0, 0, 3, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Introduction", "of", "Tax", "into", "resting", "Kit", "225", "cells", "induced", "activation", "of", "the", "G1/S", "transition", "regulation", "cascade", "consisting", "of", "activation", "of", "cyclin", "dependent", "kinase", "2", "(", "CDK2", ")", "and", "CDK4", ",", "phosphorylation", "of", "the", "Rb", "family", "proteins", "and", "an", "increase", "in", "free", "E2F", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 0, 3, 4, 0, 3, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 0], "tokens": ["The", "kinase", "activation", "was", "found", "to", "result", "from", "Tax", "-induced", "expression", "of", "genes", "for", "cell", "cycle", "regulatory", "molecules", "including", "cyclin", "D2", ",", "cyclin", "E", ",", "E2F1", ",", "CDK2", ",", "CDK4", "and", "CDK6", ",", "and", "Tax", "-induced", "reduction", "of", "CDK", "inhibitors", "p19", "(", "INK4d", ")", "and", "p27", "(", "Kip1", ")", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "modulations", "by", "Tax", "always", "paralleled", "the", "ability", "of", "Tax", "to", "activate", "the", "NF-kappaB", "transcription", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0], "tokens": ["These", "results", "indicate", "the", "important", "role", "of", "Tax", "-mediated", "trans-activation", "of", "the", "genes", "for", "cell", "cycle", "regulatory", "molecules", "in", "Tax", "-induced", "cell", "cycle", "progression", "."]} {"tags": [3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0], "tokens": ["Cot", "kinase", "induces", "cyclooxygenase-2", "expression", "in", "T", "cells", "through", "activation", "of", "the", "nuclear", "factor", "of", "activated", "T", "cells", "."]} {"tags": [3, 0, 3, 0, 0, 0, 0, 5, 6, 6, 0, 3, 4, 4, 0, 0], "tokens": ["Cyclooxygenase-2", "(", "COX-2", ")", "is", "induced", "in", "human", "T", "lymphocytes", "upon", "T", "cell", "receptor", "triggering", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 7, 8, 8, 0], "tokens": ["Here", "we", "report", "that", "Cot", "kinase", ",", "a", "mitogen-activated", "protein", "kinase", "kinase", "kinase", "involved", "in", "T", "cell", "activation", ",", "up-regulates", "COX-2", "gene", "expression", "in", "Jurkat", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0], "tokens": ["Induction", "of", "COX-2", "promoter", "activity", "by", "Cot", "kinase", "occurred", "mainly", "through", "activation", "of", "the", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NFAT", ")", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 1, 2, 2, 2, 1, 2, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Mutation", "of", "the", "distal", "(", "-105/-97", ")", "and", "proximal", "(", "-76/-61", ")", "NFAT", "response", "elements", "in", "the", "COX-2", "promoter", "abolished", "the", "activation", "induced", "by", "Cot", "kinase", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 0, 0, 0, 0, 1, 2, 0, 0, 0], "tokens": ["Even", "more", ",", "coexpression", "of", "a", "dominant", "negative", "version", "of", "NFAT", "inhibited", "Cot", "kinase", "-mediated", "COX-2", "promoter", "activation", ",", "whereas", "cotransfection", "of", "a", "constitutively", "active", "version", "of", "the", "calcium-dependent", "phosphatase", "calcineurin", "synergizes", "with", "Cot", "kinase", "in", "the", "up-regulation", "of", "COX-2", "promoter", "-driven", "transcription", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 4, 0, 3, 0], "tokens": ["Strikingly", ",", "Cot", "kinase", "increased", "transactivation", "mediated", "by", "a", "GAL4-NFAT", "fusion", "protein", "containing", "the", "N-terminal", "transactivation", "domain", "of", "NFATp", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 1, 2, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", "to", "phorbol", "ester", "plus", "calcium", "ionophore", "A23187", ",", "Cot", "kinase", "increases", "both", "COX-2", "promoter", "activity", "and", "NFAT", "-mediated", "transactivation", "in", "a", "cyclosporin", "A-independent", "manner", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 1, 2, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "indicate", "that", "Cot", "kinase", "up-regulates", "COX-2", "promoter", "-driven", "transcription", "through", "the", "NFAT", "response", "elements", ",", "being", "the", "Cot", "kinase", "-induced", "NFAT", "-dependent", "transactivation", "presumably", "implicated", "in", "this", "up-regulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 5, 6, 0], "tokens": ["Positive", "and", "negative", "roles", "of", "the", "trans-acting", "T", "cell", "factor-1", "for", "the", "acquisition", "of", "distinct", "Ly-49", "MHC", "class", "I", "receptors", "by", "NK", "cells", "."]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Members", "of", "the", "Ly-49", "gene", "family", "code", "for", "class", "I", "MHC-specific", "receptors", "that", "regulate", "NK", "cell", "function", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Due", "to", "a", "combinatorial", "distribution", "of", "Ly-49", "receptors", ",", "NK", "cells", "display", "considerable", "clonal", "heterogeneity", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 3, 4, 4, 0, 3, 0, 0], "tokens": ["The", "acquisition", "of", "one", "Ly-49", "receptor", ",", "Ly-49A", "is", "strictly", "dependent", "on", "the", "transcriptional", "trans-acting", "factor", "T", "cell-specific", "factor-1", "(", "TCF-1", ")", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 3, 0, 0], "tokens": ["Indeed", ",", "TCF-1", "binds", "to", "two", "sites", "in", "the", "Ly-49a", "promoter", "and", "regulates", "its", "activity", ",", "suggesting", "that", "the", "Ly-49a", "gene", "is", "a", "direct", "TCF-1", "target", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["TCF-1", "deficiency", "resulted", "in", "the", "altered", "usage", "of", "additional", "Ly-49", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "show", "in", "this", "study", ",", "using", "TCF-1", "beta", "(", "2", ")", "-microglobulin", "double-deficient", "mice", ",", "that", "these", "repertoire", "alterations", "are", "not", "due", "to", "Ly-49/MHC", "class", "I", "interactions", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Our", "findings", "rather", "suggest", "a", "TCF-1", "-dependent", ",", "cell", "autonomous", "effect", "on", "the", "acquisition", "of", "multiple", "Ly-49", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Besides", "reduced", "receptor", "usage", "(", "Ly-49A", "and", "D", ")", ",", "we", "also", "observed", "no", "effect", "(", "Ly-49C", ")", "and", "significantly", "expanded", "(", "Ly-49G", "and", "I", ")", "receptor", "usage", "in", "the", "absence", "of", "TCF-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 1, 2, 0], "tokens": ["These", "effects", "did", "not", "in", "all", "cases", "correlate", "with", "the", "presence", "of", "TCF", "binding", "sites", "in", "the", "respective", "proximal", "promoter", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Therefore", ",", "besides", "TCF-1", "binding", "to", "the", "proximal", "promoter", ",", "Ly-49", "acquisition", "may", "also", "be", "regulated", "by", "TCF-1", "binding", "to", "more", "distant", "cis-acting", "elements", "and/or", "by", "regulating", "the", "expression", "of", "additional", "trans-acting", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 1, 2, 0], "tokens": ["Consistent", "with", "the", "observed", "differential", ",", "positive", "or", "negative", "role", "of", "TCF-1", "for", "Ly-49", "receptor", "acquisition", ",", "reporter", "gene", "assays", "revealed", "the", "presence", "of", "an", "inducing", "as", "well", "as", "a", "repressing", "TCF", "site", "in", "certain", "proximal", "Ly-49", "promoters", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0], "tokens": ["These", "findings", "reveal", "an", "important", "role", "of", "TCF-1", "for", "the", "formation", "of", "the", "NK", "cell", "receptor", "repertoire", "."]} {"tags": [0, 0, 3, 0, 3, 4, 4, 4, 4, 3, 0, 3, 0, 3, 4, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Ligation", "of", "CD11b", "and", "CD11c", "beta", "(", "2", ")", "integrins", "by", "antibodies", "or", "soluble", "CD23", "induces", "macrophage", "inflammatory", "protein", "1alpha", "(", "MIP-1alpha", ")", "and", "MIP-1beta", "production", "in", "primary", "human", "monocytes", "through", "a", "pathway", "dependent", "on", "nuclear", "factor-kappaB", "."]} {"tags": [3, 0, 3, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["Chemokines", "and", "adhesion", "molecules", "such", "as", "integrins", "play", "a", "major", "part", "in", "the", "trafficking", ",", "extravasation", ",", "and", "recruitment", "of", "leukocytes", "to", "inflammatory", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["This", "study", "investigated", "the", "effects", "of", "beta", "(", "2", ")", "integrin", "engagement", "on", "chemokine", "production", "by", "freshly", "isolated", "human", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 4, 4, 0, 3, 4, 4, 4, 4, 0, 3, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 3, 0], "tokens": ["We", "found", "that", "ligation", "of", "CD11b", "or", "CD11c", "but", "not", "CD11a", "alpha", "chains", "of", "beta", "(", "2", ")", "integrins", "by", "antibodies", "or", "soluble", "CD23", "(", "sCD23", ")", "fusion", "proteins", "rapidly", "induced", "transcription", "and", "secretion", "of", "interleukin", "8", ",", "macrophage", "inflammatory", "protein", "(", "MIP", ")", "1alpha", ",", "and", "MIP-1beta", "."]} {"tags": [0, 0, 1, 0, 0, 1, 2, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Because", "the", "promoters", "of", "these", "chemokine", "genes", "contain", "kappaB", "binding", "sites", ",", "we", "assessed", "the", "possible", "role", "of", "nuclear", "factor-kappaB", "(", "NF-kappaB", ")", "in", "controlling", "induction", "of", "the", "genes", "through", "beta", "(", "2", ")", "integrin", "engagement", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 3, 0], "tokens": ["Electrophoretic", "mobility", "shift", "assays", "showed", "that", "sCD23", "or", "antibodies", "to", "CD11b", "or", "to", "CD11c", "up-regulated", "DNA-binding", "activity", "of", "NF-kappaB", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Activation", "of", "NF-kappaB", "was", "accompanied", "by", "degradation", "of", "its", "cytosolic", "inhibitor", "IkappaB-alpha", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Blockade", "of", "depletion", "of", "IkappaB-alpha", "by", "proteasome", "inhibitors", "(", "proteasome", "inhibitor", "I", "or", "acetyl-leucinyl-leucinyl-norleucinal", ")", "led", "to", "concomitant", "inhibition", "of", "NF-kappaB", "DNA-binding", "activity", "and", "expression", "of", "MIP-1alpha", "and", "MIP-1beta", "messenger", "RNA", "induced", "by", "beta", "(", "2", ")", "integrin", "ligation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 4, 4, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "suggest", "that", "triggering", "of", "CD11b", "or", "CD11c", "beta", "(", "2", ")", "integrin", "on", "primary", "human", "monocytes", "provides", "activation", "signals", "leading", "to", "nuclear", "translocation", "of", "NF-kappaB", "and", "subsequent", "secretion", "of", "MIP-1alpha", "and", "MIP-1beta", "that", "may", "have", "an", "important", "role", "in", "recruitment", "of", "other", "inflammatory", "cells", "during", "initiation", "of", "an", "inflammatory", "response"]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 3, 0, 0, 0, 5, 6, 0], "tokens": ["Synergistic", "transcriptional", "activation", "of", "human", "Acyl-coenzyme", "A", ":", "cholesterol", "acyltransterase-1", "gene", "by", "interferon-gamma", "and", "all-trans-retinoic", "acid", "THP-1", "cells", "."]} {"tags": [3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Acyl-coenzyme", "A", ":", "cholesterol", "acyltransferase", "(", "ACAT", ")", "is", "an", "intracellular", "enzyme", "involved", "in", "cellular", "cholesterol", "homeostasis", "and", "in", "atherosclerotic", "foam", "cell", "formation", "."]} {"tags": [1, 2, 2, 0, 0, 1, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Human", "ACAT-1", "gene", "contains", "two", "promoters", "(", "P1", "and", "P7", ")", ",", "each", "located", "in", "a", "different", "chromosome", "(", "1", "and", "7", ")", "(", "Li", ",", "B.", "L.", ",", "Li", ",", "X.", "L.", ",", "Duan", ",", "Z.", "J.", ",", "Lee", ",", "O.", ",", "Lin", ",", "S.", ",", "Ma", ",", "Z.", "M.", ",", "Chang", ",", "C.", "C.", ",", "Yang", ",", "X.", "Y.", ",", "Park", ",", "J.", "P.", ",", "Mohandas", ",", "T.", "K.", ",", "Noll", ",", "W.", ",", "Chan", ",", "L.", ",", "and", "Chang", ",", "T.", "Y.", "(", "1999", ")", "J.", "Biol", "Chem.", "274", ",", "11060-11071", ")", "."]} {"tags": [3, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 5, 6, 6, 6, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interferon-gamma", "(", "IFN-gamma", ")", ",", "a", "cytokine", "that", "exerts", "many", "pro-atherosclerotic", "effects", "in", "vivo", ",", "causes", "up-regulation", "of", "ACAT-1", "mRNA", "in", "human", "blood", "monocyte-derived", "macrophages", "and", "macrophage-like", "cells", "but", "not", "in", "other", "cell", "types", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 2, 2, 2, 0], "tokens": ["To", "examine", "the", "molecular", "nature", "of", "this", "observation", ",", "we", "identified", "within", "the", "ACAT-1", "P1", "promoter", "a", "159-base", "pair", "core", "region", "."]} {"tags": [0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["This", "region", "contains", "4", "Sp1", "elements", "and", "an", "IFN-gamma", "activated", "sequence", "(", "GAS", ")", "that", "overlaps", "with", "the", "second", "Sp1", "element", "."]} {"tags": [0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 1, 2, 0], "tokens": ["In", "the", "monocytic", "cell", "line", "THP-1", "cell", ",", "the", "combination", "of", "IFN-gamma", "and", "all-trans-retinoic", "acid", "(", "a", "known", "differentiation", "agent", ")", "enhances", "the", "ACAT-1", "P1", "promoter", "but", "not", "the", "P7", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 0], "tokens": ["Additional", "experiments", "showed", "that", "all-trans-retinoic", "acid", "causes", "large", "induction", "of", "the", "transcription", "factor", "STAT1", ",", "while", "IFN-gamma", "causes", "activation", "of", "STAT1", "such", "that", "it", "binds", "to", "the", "GAS/Sp1", "site", "in", "the", "ACAT-1", "P1", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0], "tokens": ["Our", "work", "provides", "a", "molecular", "mechanism", "to", "account", "for", "the", "effect", "of", "IFN-gamma", "in", "causing", "transcriptional", "activation", "of", "ACAT-1", "in", "macrophage-like", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhaled", "nitric", "oxide", "down-regulates", "intrapulmonary", "nitric", "oxide", "production", "in", "lipopolysaccharide-induced", "acute", "lung", "injury", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["OBJECTIVE", ":", "To", "examine", "whether", "inhaled", "nitric", "oxide", "(", "NO", ")", "affected", "the", "intrapulmonary", "production", "of", "NO", ",", "reactive", "oxygen", "species", ",", "and", "nuclear", "factor-kappaB", "in", "a", "lipopolysaccharide", "(", "LPS", ")", "-induced", "model", "of", "acute", "lung", "injury", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["DESIGN", ":", "Prospective", ",", "randomized", ",", "laboratory", "study", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["SETTING", ":", "Experimental", "laboratory", "at", "a", "biomedical", "institute", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["SUBJECTS", ":", "Twenty", "male", "rabbits", "weighing", "2.5-3.5", "kg", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["INTERVENTIONS", ":", "Saline", "or", "LPS", "(", "5", "mg/kg", "of", "body", "weight", ")", "was", "administered", "intravenously", "with", "or", "without", "NO", "inhalation", "(", "10", "ppm", ")", "in", "each", "group", "of", "five", "rabbits", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["MEASUREMENTS", "AND", "MAIN", "RESULTS", ":", "LPS", "increased", "the", "lung", "leak", "index", ",", "the", "neutrophils", "and", "NO", "levels", "in", "bronchoalveolar", "lavage", "fluid", ",", "and", "NO", "levels", "produced", "by", "resting", "and", "stimulated", "alveolar", "macrophages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Inhaled", "NO", "decreased", "the", "lung", "leak", "index", ",", "the", "neutrophils", "and", "NO", "levels", "as", "measured", "by", "nitrite", "levels", "in", "the", "lavage", "fluid", ",", "and", "NO", "produced", "by", "the", "resting", "and", "stimulated", "alveolar", "macrophages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 5, 6, 0], "tokens": ["Inhaled", "NO", "also", "blocked", "the", "activities", "of", "reactive", "oxygen", "species", "and", "nuclear", "factor-kappaB", "binding", "to", "DNA", "in", "lavage", "cells", "and", "in", "alveolar", "macrophages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSION", ":", "Inhaled", "NO", "attenuates", "LPS-induced", "acute", "lung", "injury", ",", "possibly", "by", "decreasing", "NO", "production", "in", "the", "lungs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["The", "mechanism", "of", "reducing", "NO", "production", "resulting", "from", "inhaled", "NO", "may", "involve", ",", "in", "part", ",", "the", "activities", "of", "reactive", "oxygen", "species", "and/or", "nuclear", "factor-kappaB", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0], "tokens": ["Treatment", "of", "allergic", "airway", "inflammation", "and", "hyperresponsiveness", "by", "antisense-induced", "local", "blockade", "of", "GATA-3", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 5, 6, 0, 0, 5, 6, 6, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Recent", "studies", "in", "transgenic", "mice", "have", "revealed", "that", "expression", "of", "a", "dominant", "negative", "form", "of", "the", "transcription", "factor", "GATA-3", "in", "T", "cells", "can", "prevent", "T", "helper", "cell", "type", "2", "(", "Th2", ")", "-mediated", "allergic", "airway", "inflammation", "in", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "it", "remains", "unclear", "whether", "GATA-3", "plays", "a", "role", "in", "the", "effector", "phase", "of", "allergic", "airway", "inflammation", "and", "whether", "antagonizing", "the", "expression", "and/or", "function", "of", "GATA-3", "can", "be", "used", "for", "the", "therapy", "of", "allergic", "airway", "inflammation", "and", "hyperresponsiveness", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", ",", "we", "analyzed", "the", "effects", "of", "locally", "antagonizing", "GATA-3", "function", "in", "a", "murine", "model", "of", "asthma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "could", "suppress", "GATA-3", "expression", "in", "interleukin", "(", "IL", ")", "-4", "-producing", "T", "cells", "in", "vitro", "and", "in", "vivo", "by", "an", "antisense", "phosphorothioate", "oligonucleotide", "overlapping", "the", "translation", "start", "site", "of", "GATA-3", ",", "whereas", "nonsense", "control", "oligonucleotides", "were", "virtually", "inactive", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "a", "murine", "model", "of", "asthma", "associated", "with", "allergic", "pulmonary", "inflammation", "and", "hyperresponsiveness", "in", "ovalbumin", "(", "OVA", ")", "-sensitized", "mice", ",", "local", "intranasal", "administration", "of", "fluorescein", "isothiocyanate-labeled", "GATA-3", "antisense", "oligonucleotides", "led", "to", "DNA", "uptake", "in", "lung", "cells", "associated", "with", "a", "reduction", "of", "intracellular", "GATA-3", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 5, 0, 0, 0], "tokens": ["Such", "intrapulmonary", "blockade", "of", "GATA-3", "expression", "caused", "an", "abrogation", "of", "signs", "of", "lung", "inflammation", "including", "infiltration", "of", "eosinophils", "and", "Th2", "cytokine", "production", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "treatment", "with", "antisense", "but", "not", "nonsense", "oligonucleotides", "induced", "a", "significant", "reduction", "of", "airway", "hyperresponsiveness", "in", "OVA-sensitized", "mice", "to", "levels", "comparable", "to", "saline-treated", "control", "mice", ",", "as", "assessed", "by", "both", "enhanced", "pause", "(", "PenH", ")", "responses", "and", "pulmonary", "resistance", "determined", "by", "body", "plethysmography", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "indicate", "a", "critical", "role", "for", "GATA-3", "in", "the", "effector", "phase", "of", "a", "murine", "asthma", "model", "and", "suggest", "that", "local", "delivery", "of", "GATA-3", "antisense", "oligonucleotides", "may", "be", "a", "novel", "approach", "for", "the", "treatment", "of", "airway", "hyperresponsiveness", "such", "as", "in", "asthma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["This", "approach", "has", "the", "potential", "advantage", "of", "suppressing", "the", "expression", "of", "various", "proinflammatory", "Th2", "cytokines", "simultaneously", "rather", "than", "suppressing", "the", "activity", "of", "a", "single", "cytokine", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T", "helper-cell", "phenotype", "regulates", "atherosclerosis", "in", "mice", "under", "conditions", "of", "mild", "hypercholesterolemia", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BACKGROUND", ":", "T", "cells", "are", "implicated", "in", "atherosclerosis", ",", "but", "little", "is", "known", "about", "the", "genetic", "control", "or", "molecular", "pathways", ",", "especially", "under", "conditions", "of", "mild", "hypercholesterolemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 4, 4, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0], "tokens": ["METHODS", "AND", "RESULTS", ":", "BALB/c", "mice", ",", "making", "a", "CD4+", "Th2", "(", "IL-4+", ")", "cell", "response", ",", "express", "both", "MHC", "class", "II", "antigens", "(", "IA", "(", "d", ")", ",", "IE", "(", "d", ")", ")", "and", "are", "atherosclerosis-resistant", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["C57Bl/6", "mice", "produce", "a", "CD4+", "Th1", "(", "interferon", "[", "IFN", "]", "gamma+", ")", "response", ",", "express", "IA", "(", "b", ")", "but", "no", "IE", ",", "and", "are", "atherosclerosis-prone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "evaluate", "T", "helper-cell", "phenotype", "in", "fatty", "streak", "formation", ",", "wild-type", "C57Bl/6", "mice", "(", "IA", "(", "b", ")", "+IE-", ")", "and", "transgenic", "mice", ",", "either", "AB", "(", "o", ")", ",", "IA", "(", "b", ")", "-IE-", ";", "ABEalpha", ",", "IA-IE", "(", "k", ")", "+", ";", "or", "BL", ":", "TG", ":", "Ealpha", ",", "IA", "(", "b", ")", "+IE", "(", "k", ")", "+", ",", "were", "fed", "a", "high-cholesterol", "diet", "for", "16", "weeks", "and", "evaluated", "histomorphometrically", "for", "aortic", "lesions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lesion", "size", "in", "AB", "(", "o", ")", ",", "ABEalpha", ",", "and", "BL", ":", "TG", ":", "Ealpha", "strains", "was", "decreased", "by", "54", "%", ",", "79", "%", ",", "and", "82", "%", ",", "respectively", ",", "compared", "with", "wild-type", ",", "correlating", "with", "decreased", "Th1", "and", "increased", "Th2", "expression", "and", "suggesting", "that", "T", "helper-cell", "phenotype", "is", "important", "in", "fatty", "lesion", "development", "."]} {"tags": [0, 5, 6, 0, 3, 0, 3, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Decreasing", "Th1", "cells", "by", "antibodies", "(", "alpha-CD4", ")", "or", "cytokines", "(", "IL-4", ")", "also", "caused", ">", "/=80", "%", "reductions", "in", "lesion", "size", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 5, 0], "tokens": ["Immunohistology", "revealed", "IFN-gamma", ",", "but", "not", "IL-4", ",", "colocalized", "with", "activated", "macrophages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Confirming", "these", "findings", "in", "a", "different", "mouse", "strain", ",", "BALB/c", "Stat", "6", "knockout", "mice", "(", "Th2", "cell-deficient", ")", "developed", "aortic", "lesions", "comparable", "to", "C57Bl/6", "mice", "on", "the", "same", "diet", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSIONS", ":", "In", "mildly", "hypercholesterolemic", "C57Bl/6", "mice", ",", "presence", "of", "IA", "(", "b", ")", "and", "absence", "of", "IE", "regulated", "CD4+", "T", "helper-cell", "phenotype", ";", "fatty", "lesions", "were", "proportional", "to", "IFNgamma+", "Th1", "cells", "in", "both", "C57Bl/6", "and", "BALB/c", "strains", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["IFN-gamma", "may", "participate", "through", "macrophage", "activation", ",", "whereas", "IL-4", "may", "act", "to", "limit", "Th1-cell", "response", "."]} {"tags": [0, 0, 3, 0, 3, 3, 4, 4, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Requirement", "for", "p38", "and", "p44/p42", "mitogen-activated", "protein", "kinases", "in", "RAGE", "-mediated", "nuclear", "factor-kappaB", "transcriptional", "activation", "and", "cytokine", "secretion", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Advanced", "glycation", "end", "product", "(", "AGE", ")", "activation", "of", "the", "signal-transducing", "receptor", "for", "AGE", "(", "RAGE", ")", "has", "been", "linked", "to", "a", "proinflammatory", "phenotypic", "change", "within", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "precise", "intracellular", "signaling", "pathways", "involved", "have", "not", "been", "elucidated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["We", "demonstrate", "here", "that", "human", "serum", "albumin", "modified", "with", "N", "(", "varepsilon", ")", "-", "(", "carboxymethyl", ")", "lysine", "(", "CML", ")", ",", "a", "major", "AGE", "adduct", "that", "progressively", "accumulates", "with", "aging", ",", "diabetes", ",", "and", "renal", "failure", ",", "induced", "nuclear", "factor", "(", "NF", ")", "-kappaB", "-driven", "reporter", "gene", "expression", "in", "human", "monocytic", "THP-1", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0], "tokens": ["The", "NF-kappaB", "response", "was", "blocked", "with", "a", "synthetic", "peptide", "corresponding", "to", "the", "putative", "ligand-binding", "domain", "of", "RAGE", ",", "with", "anti-", "RAGE", "antiserum", ",", "and", "by", "coexpression", "of", "truncated", "receptors", "lacking", "the", "intracellular", "domain", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Signal", "transduction", "from", "RAGE", "to", "NF-kappaB", "involved", "the", "generation", "of", "reactive", "oxygen", "species", ",", "since", "reporter", "gene", "expression", "was", "blocked", "with", "the", "antioxidant", "N-acetyl-L-cysteine", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0], "tokens": ["CML-modified", "albumin", "produced", "rapid", "transient", "activation", "of", "tyrosine", "phosphorylation", ",", "extracellular", "signal-regulated", "kinase", "1", "and", "2", ",", "and", "p38", "mitogen-activated", "protein", "kinase", "(", "MAPK", ")", ",", "but", "not", "c-Jun", "NH", "(", "2", ")", "-terminal", "kinase", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RAGE", "-mediated", "NF-kappaB", "activation", "was", "suppressed", "by", "the", "selective", "p38", "MAPK", "inhibitor", "SB203580", "and", "by", "coexpression", "of", "a", "kinase-dead", "p38", "dominant-negative", "mutant", "."]} {"tags": [0, 0, 3, 0, 3, 4, 0, 0, 0, 3, 4, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["Activation", "of", "NF-kappaB", "by", "CML-modified", "albumin", "increased", "secretion", "of", "proinflammatory", "cytokines", "(", "tumor", "necrosis", "factor-alpha", ",", "interleukin-1beta", ",", "and", "monocyte", "chemoattractant", "protein-1", ")", "severalfold", ",", "and", "inhibition", "of", "p38", "MAPK", "blocked", "these", "increases", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 3, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "indicate", "that", "p38", "MAPK", "activation", "mediates", "RAGE", "-induced", "NF-kappaB", "-dependent", "secretion", "of", "proinflammatory", "cytokines", "and", "suggest", "that", "accelerated", "inflammation", "may", "be", "a", "consequence", "of", "cellular", "activation", "induced", "by", "this", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Antigen-receptor", "cross-linking", "and", "lipopolysaccharide", "trigger", "distinct", "phosphoinositide", "3-kinase", "-dependent", "pathways", "to", "NF-kappa", "B", "activation", "in", "primary", "B", "cells", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "NF-kappaB/Rel", "transcription", "factors", "play", "an", "important", "role", "in", "the", "expression", "of", "genes", "involved", "in", "B", "cell", "development", ",", "differentiation", "and", "function", "."]} {"tags": [3, 4, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nuclear", "NF-kappaB", "is", "induced", "in", "B", "cells", "by", "engagement", "of", "either", "the", "BCR", "or", "CD40", "or", "by", "stimulation", "with", "lipopolysaccharide", "(", "LPS", ")", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Despite", "the", "importance", "of", "NF-kappaB", "to", "B", "cell", "function", ",", "little", "is", "known", "about", "the", "signaling", "pathways", "leading", "to", "NF-kappaB", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["In", "this", "report", "we", "address", "the", "role", "of", "phosphoinositide", "3'-kinase", "(", "PI", "3-kinase", ")", "in", "BCR", "-", "and", "LPS-induced", "NF-kappaB", "activation", "using", "populations", "of", "primary", "murine", "resting", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0], "tokens": ["Using", "the", "specific", "pharmacological", "inhibitors", "of", "PI", "3-kinase", ",", "Wortmannin", "and", "LY294002", ",", "we", "demonstrate", "that", "PI", "3-kinase", "activity", "is", "vital", "for", "BCR", "-induced", "NF-kappaB", "DNA-binding", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 3, 0], "tokens": ["Furthermore", ",", "we", "show", "that", "this", "is", "achieved", "via", "protein", "kinase", "C", "-dependent", "degradation", "of", "IkappaBalpha", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Similar", "analyses", "reveal", "that", "PI", "3-kinase", "is", "also", "critical", "in", "triggering", "NF-kappaB", "DNA-binding", "activity", "and", "IkappaBalpha", "degradation", "following", "LPS", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Interestingly", ",", "a", "PKC", "inhibitor", "which", "blocked", "the", "BCR", "-induced", "IkappaBalpha", "degradation", "had", "no", "effect", "on", "the", "degradation", "of", "IkappaBalpha", "after", "LPS", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0], "tokens": ["Taken", "together", ",", "our", "results", "indicate", "the", "involvement", "of", "PI", "3-kinase", "in", "at", "least", "two", "distinct", "signaling", "pathways", "leading", "to", "activation", "of", "NF-kappaB", "in", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 3, 0], "tokens": ["Tetramer-guided", "epitope", "mapping", ":", "rapid", "identification", "and", "characterization", "of", "immunodominant", "CD4+", "T", "cell", "epitopes", "from", "complex", "antigens", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 0], "tokens": ["T", "cell", "responses", "to", "Ags", "involve", "recognition", "of", "selected", "peptide", "epitopes", "contained", "within", "the", "antigenic", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 3, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0], "tokens": ["In", "this", "report", ",", "we", "describe", "a", "new", "approach", "for", "direct", "identification", "of", "CD4+", "T", "cell", "epitopes", "of", "complex", "Ags", "that", "uses", "human", "class", "II", "tetramers", "to", "identify", "reactive", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["With", "a", "panel", "of", "60", "overlapping", "peptides", "covering", "the", "entire", "sequence", "of", "the", "VP16", "protein", ",", "a", "major", "Ag", "for", "HSV-2", ",", "we", "generated", "a", "panel", "of", "class", "II", "MHC", "tetramers", "loaded", "with", "peptide", "pools", "that", "were", "used", "to", "stain", "peripheral", "lymphocytes", "of", "an", "HSV-2", "infected", "individual", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["With", "this", "approach", ",", "we", "identified", "four", "new", "DRA1*0101/DRB1*0401-", "and", "two", "DRA1*0101/DRB1*0404-restricted", ",", "VP16-specific", "epitopes", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["By", "using", "tetramers", "to", "sort", "individual", "cells", ",", "we", "easily", "obtained", "a", "large", "number", "of", "clones", "specific", "to", "these", "epitopes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "DRA1*0101/DRB1*0401", "and", "DRA1*0101/DRB1*0404", "are", "structurally", "very", "similar", ",", "nonoverlapping", "VP16", "epitopes", "were", "identified", ",", "illustrating", "high", "selectivity", "of", "individual", "allele", "polymorphisms", "within", "common", "MHC", "variants", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "rapid", "approach", "to", "detecting", "CD4+", "T", "cell", "epitopes", "from", "complex", "Ags", "can", "be", "applied", "to", "any", "known", "Ag", "that", "gives", "a", "T", "cell", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Localized", "pancreatic", "NF-kappaB", "activation", "and", "inflammatory", "response", "in", "taurocholate-induced", "pancreatitis", "."]} {"tags": [3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transcription", "factor", "nuclear", "factor-kappaB", "(", "NF-kappaB", ")", "is", "activated", "in", "cerulein", "pancreatitis", "and", "mediates", "cytokine", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "role", "of", "transcription", "factor", "activation", "in", "other", "models", "of", "pancreatitis", "has", "not", "been", "established", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "report", "upregulation", "of", "NF-kappaB", "and", "inflammatory", "molecules", ",", "and", "their", "correlation", "with", "local", "pancreatic", "injury", ",", "in", "a", "model", "of", "severe", "pancreatitis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Rats", "received", "intraductal", "infusion", "of", "taurocholate", "or", "saline", ",", "and", "the", "pancreatic", "head", "and", "tail", "were", "analyzed", "separately", "."]} {"tags": [3, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 3, 4, 4, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["NF-kappaB", "and", "activator", "protein-1", "(", "AP-1", ")", "activation", "were", "assessed", "by", "gel", "shift", "assay", ",", "and", "mRNA", "expression", "of", "interleukin-6", ",", "tumor", "necrosis", "factor-alpha", ",", "KC", ",", "monocyte", "chemoattractant", "protein-1", ",", "and", "inducible", "nitric", "oxide", "synthase", "was", "assessed", "by", "semiquantitative", "RT-PCR", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0], "tokens": ["Morphological", "damage", "and", "trypsin", "activation", "were", "much", "greater", "in", "the", "pancreatic", "head", "than", "tail", ",", "in", "parallel", "with", "a", "stronger", "activation", "of", "NF-kappaB", "and", "cytokine", "mRNA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Saline", "infusion", "mildly", "affected", "these", "parameters", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["AP-1", "was", "strongly", "activated", "in", "both", "pancreatic", "segments", "after", "either", "taurocholate", "or", "saline", "infusion", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NF-kappaB", "inhibition", "with", "N-acetylcysteine", "ameliorated", "the", "local", "inflammatory", "response", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Correlation", "between", "localized", "NF-kappaB", "activation", ",", "cytokine", "upregulation", ",", "and", "tissue", "damage", "suggests", "a", "key", "role", "for", "NF-kappaB", "in", "the", "development", "of", "the", "inflammatory", "response", "of", "acute", "pancreatitis", "."]} {"tags": [3, 4, 4, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CD45", "tyrosine", "phosphatase", "controls", "common", "gamma-chain", "cytokine", "-mediated", "STAT", "and", "extracellular", "signal-related", "kinase", "phosphorylation", "in", "activated", "human", "lymphoblasts", ":", "inhibition", "of", "proliferation", "without", "induction", "of", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["The", "objective", "of", "this", "study", "was", "to", "test", "whether", "CD45", "signals", "can", "influence", "signaling", "processes", "in", "activated", "human", "lymphoblasts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "this", "end", ",", "we", "generated", "lymphoblasts", "which", "proliferate", "in", "response", "to", "common", "gamma-chain", "cytokines", ",", "but", "readily", "undergo", "apoptosis", "after", "cytokine", "withdrawal", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "experiments", "with", "the", "CD45R0", "mAb", "UCHL-1", ",", "but", "not", "control", "CD45", "mAbs", ",", "we", "found", "significant", "inhibition", "of", "proliferation", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 5, 0], "tokens": ["Interestingly", ",", "the", "pan-CD45", "mAb", "GAP8.3", ",", "which", "is", "most", "effective", "in", "inhibition", "of", "OKT-3-mediated", "proliferation", "in", "quiescent", "lymphocytes", ",", "was", "ineffective", "in", "lymphoblasts", "."]} {"tags": [0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Addition", "of", "CD3", "mAb", "OKT-3", "had", "no", "influence", "on", "IL-2-mediated", "proliferation", "(", "with", "or", "without", "UCHL-1", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "after", "addition", "of", "OKT-3", "to", "IL-4", "-", "and", "IL-7", "-stimulated", "proliferation", "assays", ",", "UCHL-1", "signals", "could", "not", "significantly", "alter", "cellular", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "did", "not", "find", "induction", "of", "apoptosis", "following", "CD45R0", "signaling", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 4, 4, 4, 4, 4, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "Western", "blots", "using", "mAbs", "detecting", "phosphorylated", "STAT-3", ",", "STAT-5", ",", "STAT-6", ",", "or", "extracellular", "signal-related", "kinase", "1/2", ",", "we", "found", "that", "CD45R0", "signaling", "could", "effectively", "diminish", "phosphorylation", "of", "these", "intracellular", "signaling", "components", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 9, 10, 0, 0], "tokens": ["Using", "RT-PCR", ",", "we", "found", "that", "CD45R0", "signaling", "inhibited", "IL-2", "mRNA", "production", "without", "major", "influence", "on", "IL-13", ",", "IL-5", ",", "or", "IFN-gamma", "mRNA", "levels", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Costimulation", "with", "OKT-3", "and", "IL-2", "optimally", "induced", "secretion", "of", "IFN-gamma", ",", "TNF-alpha", ",", "and", "IL-5", ",", "which", "was", "not", "decreased", "by", "CD45", "signals", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["In", "conclusion", ",", "we", "illustrate", "that", "CD45R0", "signals", "control", "early", "cytokine", "receptor-associated", "signaling", "processes", "and", "mRNA", "and", "DNA", "synthesis", "in", "activated", "human", "lymphoblasts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Furthermore", ",", "we", "show", "the", "existence", "of", "CD45", "epitopes", "(", "GAP8.3", ")", ",", "which", "are", "active", "and", "critical", "for", "signaling", "in", "quiescent", "lymphocytes", ",", "but", "are", "nonfunctional", "in", "activated", "human", "lymphoblasts", "."]} {"tags": [1, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Pax5", "determines", "the", "identity", "of", "B", "cells", "from", "the", "beginning", "to", "the", "end", "of", "B-lymphopoiesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Despite", "being", "one", "of", "the", "most", "intensively", "studied", "cell", "types", ",", "the", "molecular", "basis", "of", "B", "cell", "specification", "is", "largely", "unknown", "."]} {"tags": [0, 1, 2, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "Pax5", "gene", "encoding", "the", "transcription", "factor", "BSAP", "is", "required", "for", "progression", "of", "B-lymphopoiesis", "beyond", "the", "pro-B", "cell", "stage", "."]} {"tags": [5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Pax5-deficient", "pro-B", "cells", "are", ",", "however", ",", "not", "yet", "committed", "to", "the", "B-lymphoid", "lineage", ",", "but", "instead", "have", "a", "broad", "lymphomyeloid", "developmental", "potential", "."]} {"tags": [1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Pax5", "appears", "to", "mediate", "B-lineage", "commitment", "by", "repressing", "the", "transcription", "of", "non-B-lymphoid", "genes", "and", "by", "simultaneously", "activating", "the", "expression", "of", "B-lineage-specific", "genes", "."]} {"tags": [1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Pax5", "thus", "functions", "both", "as", "a", "transcriptional", "repressor", "and", "activator", ",", "depending", "on", "its", "interactions", "with", "corepressors", "of", "the", "Groucho", "protein", "family", "or", "with", "positive", "regulators", "such", "as", "the", "TATA-binding", "protein", "."]} {"tags": [0, 0, 0, 0, 5, 0, 5, 6, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Once", "committed", "to", "the", "B-lineage", ",", "B", "cells", "require", "Pax5", "function", "to", "maintain", "their", "B-lymphoid", "identity", "throughout", "B", "cell", "development"]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Partners", "in", "transcription", ":", "NFAT", "and", "AP-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Combinatorial", "regulation", "is", "a", "powerful", "mechanism", "that", "enables", "tight", "control", "of", "gene", "expression", ",", "via", "integration", "of", "multiple", "signaling", "pathways", "that", "induce", "different", "transcription", "factors", "required", "for", "enhanceosome", "assembly", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "four", "calcium-regulated", "transcription", "factors", "of", "the", "NFAT", "family", "act", "synergistically", "with", "AP-1", "(", "Fos/Jun", ")", "proteins", "on", "composite", "DNA", "elements", "which", "contain", "adjacent", "NFAT", "and", "AP-1", "binding", "sites", ",", "where", "they", "form", "highly", "stable", "ternary", "complexes", "to", "regulate", "the", "expression", "of", "diverse", "inducible", "genes", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 0], "tokens": ["Concomitant", "induction", "of", "NFAT", "and", "AP-1", "requires", "concerted", "activation", "of", "two", "different", "signaling", "pathways", ":", "calcium/calcineurin", ",", "which", "promotes", "NFAT", "dephosphorylation", ",", "nuclear", "translocation", "and", "activation", ";", "and", "protein", "kinase", "C", "(", "PKC", ")", "/Ras", ",", "which", "promotes", "the", "synthesis", ",", "phosphorylation", "and", "activation", "of", "members", "of", "the", "Fos", "and", "Jun", "families", "of", "transcription", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0], "tokens": ["A", "fifth", "member", "of", "the", "NFAT", "family", ",", "NFAT5", ",", "controls", "the", "cellular", "response", "to", "osmotic", "stress", ",", "by", "a", "mechanism", "that", "requires", "dimer", "formation", "and", "is", "independent", "of", "calcineurin", "or", "of", "interaction", "with", "AP-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Pharmacological", "interference", "with", "theNFAT", ":", "AP-1", "interaction", "may", "be", "useful", "in", "selective", "manipulation", "of", "the", "immune", "response", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Balanced", "activation", "of", "NFAT", "and", "AP-1", "is", "known", "to", "be", "required", "for", "productive", "immune", "responses", ",", "but", "the", "role", "of", "NFAT", ":", "AP-1", "interactions", "in", "other", "cell", "types", "and", "biological", "processes", "remains", "to", "be", "understood", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cytokine", "production", "by", "Vgamma", "(", "+", ")", "-T-cell", "subsets", "is", "an", "important", "factor", "determining", "CD4", "(", "+", ")", "-Th-cell", "phenotype", "and", "susceptibility", "of", "BALB/c", "mice", "to", "coxsackievirus", "B3-induced", "myocarditis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Two", "coxsackievirus", "B3", "(", "CVB3", ")", "variants", "(", "H3", "and", "H310A1", ")", "differ", "by", "a", "single", "amino", "acid", "mutation", "in", "the", "VP2", "capsid", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["H3", "induces", "severe", "myocarditis", "in", "BALB/c", "mice", ",", "but", "H310A1", "is", "amyocarditic", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Infection", "with", "H3", ",", "but", "not", "H310A1", ",", "preferentially", "activates", "Vgamma4", "Vdelta4", "cells", ",", "which", "are", "strongly", "positive", "for", "gamma", "interferon", "(", "IFN-gamma", ")", ",", "whereas", "Vgamma1", "Vdelta4", "cells", "are", "increased", "in", "both", "H3", "and", "H310A1", "virus-infected", "animals", "."]} {"tags": [0, 0, 5, 6, 6, 6, 6, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Depletion", "of", "Vgamma1", "(", "+", ")", "cells", "using", "monoclonal", "anti-Vgamma1", "antibody", "enhanced", "myocarditis", "and", "CD4", "(", "+", ")", "-", ",", "IFN-gamma", "(", "+", ")", "-cell", "responses", "in", "both", "H3-", "and", "H310A1-infected", "mice", "yet", "decreased", "the", "CD4", "(", "+", ")", "-", ",", "IL-4", "(", "+", ")", "-cell", "response", "."]} {"tags": [0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0], "tokens": ["Depleting", "Vgamma4", "(", "+", ")", "cells", "suppressed", "myocarditis", "and", "reduced", "CD4", "(", "+", ")", "IFN-gamma", "(", "+", ")", "cells", "but", "increased", "CD4", "(", "+", ")", "IL-4", "(", "+", ")", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "role", "of", "cytokine", "production", "by", "Vgamma1", "(", "+", ")", "and", "Vgamma4", "(", "+", ")", "T", "cells", "was", "investigated", "by", "adoptively", "transferring", "these", "cells", "isolated", "from", "H3-infected", "BALB/c", "Stat4", "knockout", "(", "Stat4ko", ")", "(", "defective", "in", "IFN-gamma", "expression", ")", "or", "BALB/c", "Stat6ko", "(", "defective", "in", "IL-4", "expression", ")", "mice", "into", "H3", "virus-infected", "wild-type", "BALB/c", "recipients", "."]} {"tags": [5, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0], "tokens": ["Vgamma4", "and", "Vgamma1", "(", "+", ")", "T", "cells", "from", "Stat4ko", "mice", "expressed", "IL-4", "but", "no", "or", "minimal", "IFN-gamma", ",", "whereas", "these", "cell", "populations", "derived", "from", "Stat6ko", "mice", "expressed", "IFN-gamma", "but", "no", "IL-4", "."]} {"tags": [5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0], "tokens": ["Stat4ko", "Vgamma1", "(", "+", ")", "cells", "(", "IL-4", "(", "+", ")", ")", "suppress", "myocarditis", "."]} {"tags": [5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0], "tokens": ["Stat6ko", "Vgamma1", "(", "+", ")", "cells", "(", "IFN-gamma", "(", "+", ")", ")", "were", "not", "inhibitory", "."]} {"tags": [5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0], "tokens": ["Stat6ko", "Vgamma4", "(", "+", ")", "cells", "(", "IFN-gamma", "(", "+", ")", ")", "significantly", "enhanced", "myocarditis", "."]} {"tags": [5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Stat4ko", "Vgamma4", "(", "+", ")", "cells", "(", "IL-4", "(", "+", ")", ")", "neither", "inhibited", "nor", "enhanced", "disease", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0], "tokens": ["These", "results", "show", "that", "distinct", "gammadelta-T-cell", "subsets", "control", "myocarditis", "susceptibility", "and", "bias", "the", "CD4", "(", "+", ")", "-Th-cell", "response", "."]} {"tags": [0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["The", "cytokines", "produced", "by", "the", "Vgamma", "subpopulation", "have", "a", "significant", "influence", "on", "the", "CD4", "(", "+", ")", "-Th-cell", "phenotype", "."]} {"tags": [3, 0, 0, 0, 3, 0, 3, 4, 0, 5, 6, 0, 3, 0, 3, 0, 0, 0, 0], "tokens": ["Plasmin", "-induced", "expression", "of", "cytokines", "and", "tissue", "factor", "in", "human", "monocytes", "involves", "AP-1", "and", "IKKbeta", "-mediated", "NF-kappaB", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "was", "previously", "shown", "that", "plasmin", "activates", "human", "peripheral", "monocytes", "in", "terms", "of", "lipid", "mediator", "release", "and", "chemotactic", "migration", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 3, 4, 0, 3, 0, 0, 0, 5, 0], "tokens": ["Here", "it", "is", "demonstrated", "that", "plasmin", "induces", "proinflammatory", "cytokine", "release", "and", "tissue", "factor", "(", "TF", ")", "expression", "by", "monocytes", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 3, 4, 4, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Plasmin", "0.043", "to", "1.43", "CTA", "U/mL", ",", "but", "not", "active", "site-blocked", "plasmin", ",", "triggered", "concentration-dependent", "expression", "of", "mRNA", "for", "interleukin-1alpha", "(", "IL-1alpha", ")", ",", "IL-1beta", ",", "tumor", "necrosis", "factor-alpha", "(", "TNF-alpha", ")", ",", "and", "TF", "with", "maximum", "responses", "after", "4", "hours", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Plasmin", "-mediated", "mRNA", "expression", "was", "inhibited", "in", "a", "concentration-dependent", "manner", "by", "the", "lysine", "analogue", "trans-4-", "(", "aminomethyl", ")", "cyclohexane-1-carboxylic", "acid", "(", "t-AMCA", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Increases", "in", "mRNA", "levels", "were", "followed", "by", "concentration-", "and", "time-dependent", "release", "of", "IL-1alpha", ",", "IL-1beta", "and", "TNF-alpha", "and", "by", "TF", "expression", "on", "monocyte", "surfaces", "."]} {"tags": [0, 3, 0, 3, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Neither", "cytokines", "nor", "TF", "could", "be", "detected", "when", "monocytes", "were", "preincubated", "with", "actinomycin", "D", "or", "cycloheximide", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Electrophoretic", "mobility", "shift", "assays", "indicated", "plasmin", "-induced", "activation", "of", "NF-kappaB", ";", "DNA-binding", "complexes", "were", "composed", "of", "p50", ",", "p65", ",", "and", "c-Rel", ",", "as", "shown", "by", "supershift", "experiments", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Nuclear", "translocation", "of", "NF-kappaB/Rel", "proteins", "coincided", "with", "IkappaBalpha", "degradation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["At", "variance", "with", "endotoxic", "lipopolysaccharide", ",", "plasmin", "elicited", "the", "rapid", "degradation", "of", "another", "cytoplasmic", "NF-kappaB", "inhibitor", ",", "p105", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Proteolysis", "of", "NF-kappaB", "inhibitors", "was", "apparently", "due", "to", "transient", "activation", "of", "IkappaB", "kinase", "(", "IKK", ")", "beta", "that", "reached", "maximum", "activity", "at", "1", "hour", "after", "plasmin", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0], "tokens": ["In", "addition", ",", "AP-1", "binding", "was", "increased", "in", "plasmin", "-treated", "monocytes", ",", "with", "most", "complexes", "composed", "of", "JunD", ",", "c-Fos", ",", "and", "FosB", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "findings", "further", "substantiate", "the", "role", "of", "plasmin", "as", "a", "proinflammatory", "activator", "of", "human", "monocytes", "and", "reveal", "an", "important", "new", "link", "between", "the", "plasminogen-plasmin", "system", "and", "inflammation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["(", "Blood.", "2001", ";", "97", ":", "3941-3950", ")"]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["STAT3", "is", "constitutively", "active", "in", "some", "patients", "with", "Polycythemia", "rubra", "vera", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0], "tokens": ["OBJECTIVE", ":", "Polycythemia", "vera", "is", "a", "clonal", "stem", "cell", "disorder", "characterized", "by", "hyperproliferation", "of", "the", "erythroid", ",", "myeloid", ",", "and", "megakaryocytic", "lineages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["While", "it", "has", "been", "shown", "that", "progenitor", "cells", "of", "P.", "vera", "patients", "are", "hypersensitive", "to", "several", "growth", "factors", "including", "erythropoietin", ",", "insulin-like", "growth", "factor-1", ",", "thrombopoietin", ",", "interleukin-3", ",", "and", "granulocyte/monocyte", "colony-stimulating", "factor", ",", "the", "molecular", "pathogenesis", "of", "this", "disease", "remains", "unknown", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Growth", "factor", "hypersensitivity", "could", "be", "mediated", "by", "changes", "in", "signal", "transduction", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0], "tokens": ["We", "therefore", "investigated", "a", "common", "downstream", "effector", "of", "cytokines", ",", "the", "signal", "transducers", "and", "activators", "of", "transcription", "(", "STATs", ")", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "constitutive", "activation", "of", "STAT", "factors", "could", "explain", "the", "increased", "proliferation", "of", "P.", "vera", "cells", "even", "in", "the", "absence", "of", "growth", "factor", "stimulation", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Peripheral", "granulocytes", "from", "patients", "with", "P.", "vera", "and", "from", "healthy", "volunteers", "were", "assayed", "for", "STAT1", ",", "3", ",", "and", "5", "DNA", "binding", "by", "electrophoretic", "mobility", "shift", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "Four", "of", "14", "P.", "vera", "patients", "analyzed", "showed", "constitutive", "STAT3", "DNA", "binding", "in", "unstimulated", "peripheral", "granulocytes", ",", "while", "none", "of", "the", "17", "healthy", "volunteers", "tested", "did", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0], "tokens": ["of", "the", "subjects", "showed", "constitutive", "STAT1", "or", "STAT5", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Western", "blotting", "demonstrated", "that", ",", "in", "the", "three", "patients", ",", "STAT3", "is", "constitutively", "phosphorylated", "on", "Tyr", "705", ",", "whereas", "it", "is", "unphosphorylated", "in", "the", "other", "patients", "and", "in", "controls", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interestingly", ",", "constitutive", "STAT3", "activity", "did", "not", "correlate", "with", "the", "duration", "of", "disease", "or", "the", "treatment", "regimen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "was", "observed", "in", "a", "recently", "diagnosed", "patient", "and", "in", "two", "patients", "treated", "only", "with", "phlebotomy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSION", ":", "Our", "data", "suggest", "that", "constitutive", "phosphorylation", "and", "activation", "of", "STAT3", "is", "not", "a", "secondary", "event", "induced", "by", "mutagenizing", "agents", "or", "by", "prolonged", "hyperproliferation", "of", "hematopoietic", "cells", ",", "but", "rather", "represents", "a", "primary", "molecular", "aberration", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Constitutively", "active", "STAT3", "may", "contribute", "to", "the", "growth", "factor", "hypersensitivity", "of", "P.", "vera", "cells", "."]} {"tags": [0, 0, 3, 4, 0, 3, 4, 4, 4, 0, 0, 3, 4, 4, 0], "tokens": ["Identification", "of", "phosphorylation", "sites", "for", "Bruton", "'s", "tyrosine", "kinase", "within", "the", "transcriptional", "regulator", "BAP/TFII-I", "."]} {"tags": [3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Bruton", "'s", "tyrosine", "kinase", "(", "Btk", ")", ",", "a", "member", "of", "the", "Tec", "family", "of", "cytosolic", "kinases", ",", "is", "essential", "for", "B", "cell", "development", "and", "function", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["BAP/TFII-I", ",", "a", "protein", "implicated", "in", "transcriptional", "regulation", ",", "is", "associated", "with", "Btk", "in", "B", "cells", "and", "is", "transiently", "phosphorylated", "on", "tyrosine", "following", "B", "cell", "receptor", "engagement", "."]} {"tags": [3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0], "tokens": ["BAP/TFII-I", "is", "a", "substrate", "for", "Btk", "in", "vitro", "and", "is", "hyperphosphorylated", "on", "tyrosine", "upon", "coexpression", "with", "Btk", "in", "mammalian", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0], "tokens": ["In", "an", "effort", "to", "understand", "the", "physiologic", "consequences", "of", "BAP/TFII-I", "tyrosine", "phosphorylation", "following", "B", "cell", "receptor", "stimulation", ",", "site-directed", "mutagenesis", "and", "phosphopeptide", "mapping", "were", "used", "to", "locate", "the", "predominant", "sites", "of", "BAP/TFII-I", "phosphorylation", "by", "Btk", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0], "tokens": ["These", "residues", ",", "Tyr248", ",", "Tyr357", ",", "and", "Tyr462", ",", "were", "also", "found", "to", "be", "the", "major", "sites", "for", "Btk", "-dependent", "phosphorylation", "of", "BAP/TFII-I", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 3, 0], "tokens": ["Residues", "Tyr357", "and", "Tyr462", "are", "contained", "within", "the", "loop", "regions", "of", "adjacent", "helix-loop-helix-like", "repeats", "within", "BAP/TFII-I", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mutation", "of", "either", "Tyr248", ",", "Tyr357", ",", "or", "Tyr462", "to", "phenylalanine", "reduced", "transcription", "from", "a", "c-fos", "promoter", "relative", "to", "wild-type", "BAP/TFII-I", "in", "transfected", "COS-7", "cells", ",", "consistent", "with", "the", "interpretation", "that", "phosphorylation", "at", "these", "sites", "contributes", "to", "transcriptional", "activation", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Phosphorylation", "of", "BAP/TFII-I", "by", "Btk", "may", "link", "engagement", "of", "receptors", "such", "as", "surface", "immunoglobulin", "to", "modulation", "of", "gene", "expression", "."]} {"tags": [0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Expression", "of", "interferon", "consensus", "sequence", "binding", "protein", "induces", "potent", "immunity", "against", "BCR/ABL-induced", "leukemia", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Mice", "deficient", "in", "the", "interferon", "consensus", "sequence", "binding", "protein", "(", "ICSBP", ")", "develop", "a", "disease", "resembling", "chronic", "myeloid", "leukemia", "(", "CML", ")", ",", "which", "in", "humans", "is", "caused", "by", "the", "BCR/ABL", "oncoprotein", "."]} {"tags": [3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interferon-alpha", "(", "IFN-alpha", ")", "induces", "ICSBP", "expression", "and", "is", "an", "effective", "therapy", "for", "CML", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["This", "study", "examined", "whether", "enforced", "expression", "of", "ICSBP", "might", "antagonize", "BCR/ABL-induced", "leukemia", ";", "results", "demonstrated", "that", "ICSBP-modified", "cells", "generated", "a", "protective", "CD8", "(", "+", ")", "cytotoxic", "T-cell", "response", "against", "BCR/ABL-transformed", "BaF3", "cells", "in", "a", "murine", "leukemia", "model", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["ICSBP", "expression", "represents", "a", "novel", "means", "of", "stimulating", "a", "host", "immune", "response", "to", "BCR/ABL", "(", "+", ")", "leukemia", "cells", "and", "a", "potential", "strategy", "for", "immunotherapy", "of", "CML", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["(", "Blood.", "2001", ";", "97", ":", "3491-3497", ")"]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 0, 5, 6, 6, 6, 0], "tokens": ["A", "transcriptional", "block", "in", "the", "IL-2", "promoter", "at", "the", "-150", "AP-1", "site", "in", "effector", "CD8+", "T", "cells", "."]} {"tags": [0, 5, 6, 6, 6, 6, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Both", "CD4+", "and", "CD8+", "T", "cells", "that", "produce", "IL-2", "in", "response", "to", "Ag", "recognition", "have", "been", "isolated", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "most", "effector", "CD8+", "T", "cells", "recovered", "after", "exposure", "to", "Ag", "do", "not", "produce", "sufficient", "IL-2", "to", "sustain", "growth", ",", "and", "depend", "on", "CD4+", "T", "helper", "cells", "for", "this", "obligate", "growth", "factor", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0], "tokens": ["IL-2", "expression", "in", "CD4+", "T", "cells", "is", "primarily", "controlled", "at", "the", "level", "of", "transcription", ",", "but", "mechanisms", "restricting", "IL-2", "production", "in", "CD8+", "T", "cells", "have", "not", "been", "elucidated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 6, 0, 0, 0, 0, 1, 2, 0, 3, 0, 7, 8, 8, 8, 0], "tokens": ["To", "evaluate", "transcriptional", "regulation", "of", "the", "IL-2", "gene", "in", "CD8+", "T", "cells", ",", "we", "stably", "transfected", "reporter", "genes", "into", "Ag", "-specific", "CD8+", "T", "cell", "clones", "."]} {"tags": [5, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 1, 2, 2, 0], "tokens": ["CD28+", "CD8", "(", "+", ")", "T", "cells", "unable", "to", "transcribe", "the", "IL-2", "gene", "in", "response", "to", "antigenic", "stimulation", "had", "a", "block", "in", "transactivation", "of", "the", "-150", "CD28", "response", "element", "(", "CD28RE", ")", "/AP-1", "site", "of", "the", "IL-2", "promoter", ",", "but", "did", "transactivate", "the", "composite", "NFAT/AP-1", "and", "OCT/AP-1", "sites", ",", "and", "a", "consensus", "AP-1", "motif", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 1, 0], "tokens": ["Mutation", "of", "the", "nonconsensus", "-150", "AP-1", "site", "to", "a", "consensus", "AP-1", "site", ",", "or", "insertion", "of", "a", "CD28RE/AP-1", "consensus", "site", "upstream", "of", "the", "native", "-150", "CD28RE/AP-1", "site", "restored", "transactivation", "of", "the", "altered", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["These", "results", "suggest", "that", "the", "defect", "at", "the", "-150", "site", "may", "reflect", "the", "absence", "or", "inactivity", "of", "a", "required", "factor", "rather", "than", "repression", "of", "the", "IL-2", "promoter", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 3, 4, 4, 0, 7, 8, 8, 8, 8, 8, 0], "tokens": ["Stem", "cell", "factor", "and", "interleukin-3", "induce", "stepwise", "generation", "of", "erythroid", "precursor", "cells", "from", "a", "basic", "fibroblast", "growth", "factor", "-dependent", "hematopoietic", "stem", "cell", "line", ",", "A-6", "."]} {"tags": [0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 7, 8, 8, 8, 8, 8, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 0], "tokens": ["A", "m", "ultipotent", "immature", "myeloid", "cell", "population", "was", "produced", "from", "a", "basic", "fibroblast", "growth", "factor", "(", "bFGF", ")", "-dependent", "hematopoietic", "stem", "cell", "line", ",", "A-6", ",", "when", "cultured", "with", "stem", "cell", "factor", "(", "SCF", ")", "replacing", "bFGF", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["Those", "cells", "were", "positive", "for", "stem", "cell", "markers", ",", "c-kit", "and", "CD34", ",", "and", "a", "myeloid", "cell", "marker", ",", "F4/80", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Some", "cell", "fractions", "were", "also", "positive", "for", "Mac-1", ",", "a", "macrophage", "marker", "or", "Gr-1", ",", "a", "granulocytic", "maker", ",", "but", "negative", "for", "an", "erythroid", "marker", "TER119", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["They", "also", "showed", "the", "expression", "of", "mRNA", "for", "the", "myeloid-specific", "PU.1", "but", "did", "not", "that", "for", "the", "erythroid-specific", "GATA-1", "."]} {"tags": [0, 0, 3, 0, 3, 0, 3, 0, 0, 5, 6, 6, 0, 0, 0, 3, 4, 0, 3, 4, 0], "tokens": ["Among", "various", "cytokines", ",", "interleukin-3", "(", "IL-3", ")", "induced", "erythroid", "precursor", "cells", "that", "expressed", "the", "erythroid-specific", "GATA-1", "and", "beta-major", "globin", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 3, 0], "tokens": ["The", "quantitative", "analysis", "showed", "that", "erythroid", "precursor", "cells", "were", "newly", "produced", "from", "the", "immature", "myeloid", "cells", "by", "cultivation", "with", "IL-3", "."]} {"tags": [3, 0, 3, 0, 0, 0, 0, 5, 6, 6, 0, 0, 7, 8, 8, 8, 8, 0], "tokens": ["SCF", "and", "IL-3", "induced", "stepwise", "generation", "of", "erythroid", "precursor", "cells", "from", "an", "A-6", "hematopoietic", "stem", "cell", "line", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Copyright", "2001", "Academic", "Press", "."]} {"tags": [0, 3, 0, 3, 0, 0, 0, 5, 0, 5, 6, 6, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0], "tokens": ["Distinct", "BMI-1", "and", "EZH2", "expression", "patterns", "in", "thymocytes", "and", "mature", "T", "cells", "suggest", "a", "role", "for", "Polycomb", "genes", "in", "human", "T", "cell", "differentiation", "."]} {"tags": [3, 0, 3, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BMI-1", "and", "EZH2", "Polycomb-group", "(", "PcG", ")", "proteins", "belong", "to", "two", "distinct", "protein", "complexes", "involved", "in", "the", "regulation", "of", "hematopoiesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 3, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "unique", "PcG-specific", "antisera", "and", "triple", "immunofluorescence", ",", "we", "found", "that", "mature", "resting", "peripheral", "T", "cells", "expressed", "BMI-1", ",", "whereas", "dividing", "blasts", "were", "EZH2", "(", "+", ")", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["By", "contrast", ",", "subcapsular", "immature", "double-negative", "(", "DN", ")", "(", "CD4", "(", "-", ")", "/CD8", "(", "-", ")", ")", "T", "cells", "in", "the", "thymus", "coexpressed", "BMI-1", "and", "EZH2", "or", "were", "BMI-1", "single", "positive", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 3, 0, 3, 0], "tokens": ["Their", "descendants", ",", "double-positive", "(", "DP", ";", "CD4", "(", "+", ")", "/CD8", "(", "+", ")", ")", "cortical", "thymocytes", ",", "expressed", "EZH2", "without", "BMI-1", "."]} {"tags": [0, 5, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0], "tokens": ["Most", "EZH2", "(", "+", ")", "DN", "and", "DP", "thymocytes", "were", "dividing", ",", "while", "DN", "BMI-1", "(", "+", ")", "/EZH2", "(", "-", ")", "thymocytes", "were", "resting", "and", "proliferation", "was", "occasionally", "noted", "in", "DN", "BMI-1", "(", "+", ")", "/EZH2", "(", "+", ")", "cells", "."]} {"tags": [0, 0, 5, 6, 6, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Maturation", "of", "DP", "cortical", "thymocytes", "to", "single-positive", "(", "CD4", "(", "+", ")", "/CD8", "(", "-", ")", "or", "CD8", "(", "+", ")", "/CD4", "(", "-", ")", ")", "medullar", "thymocytes", "correlated", "with", "decreased", "detectability", "of", "EZH2", "and", "continued", "relative", "absence", "of", "BMI-1", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "data", "show", "that", "BMI-1", "and", "EZH2", "expression", "in", "mature", "peripheral", "T", "cells", "is", "mutually", "exclusive", "and", "linked", "to", "proliferation", "status", ",", "and", "that", "this", "pattern", "is", "not", "yet", "established", "in", "thymocytes", "of", "the", "cortex", "and", "medulla", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T", "cell", "stage-specific", "PcG", "expression", "profiles", "suggest", "that", "PcG", "genes", "contribute", "to", "regulation", "of", "T", "cell", "differentiation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["They", "probably", "reflect", "stabilization", "of", "cell", "type-specific", "gene", "expression", "and", "irreversibility", "of", "lineage", "choice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "difference", "in", "PcG", "expression", "between", "medullar", "thymocytes", "and", "mature", "interfollicular", "T", "cells", "indicates", "that", "additional", "maturation", "processes", "occur", "after", "thymocyte", "transportation", "from", "the", "thymus", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 0], "tokens": ["Stepwise", "lineage", "restriction", "of", "progenitors", "in", "lympho-myelopoiesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "has", "long", "been", "controversial", "whether", "hematopoiesis", "progresses", "through", "ordered", "stages", "of", "determination", "as", "in", "embryonic", "development", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["This", "is", "due", "to", "the", "absence", "of", "a", "methodology", "capable", "of", "exactly", "determining", "the", "developmental", "potential", "of", "hematopoietic", "stem/progenitor", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "multilineage", "progenitor", "(", "MLP", ")", "assay", "enabled", "us", "to", "discriminate", "among", "seven", "types", "of", "hematopoietic", "progenitors", ",", "which", "are", "multipotent", "progenitor", "p-MTB", "(", "capable", "of", "generating", "myeloid", ",", "T", "and", "B", "cells", ")", ",", "bipotent", "progenitors", "p-MT", ",", "p-MB", "and", "p-TB", ",", "and", "unipotent", "progenitors", "p-M", ",", "p-T", "and", "p-B", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Among", "these", "seven", "types", ",", "the", "p-TB", "type", "progenitor", "was", "found", "to", "be", "absent", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "findings", "indicate", "that", "the", "process", "of", "lineage", "commitment", "proceeds", "through", "an", "ordered", "but", "not", "random", "process", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["By", "extending", "the", "area", "of", "investigation", "to", "include", "the", "erythroid", "lineage", ",", "more", "convincing", "evidence", "for", "the", "ordered", "process", "was", "obtained", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Detailed", "and", "exact", "illustration", "of", "the", "process", "of", "hematopoiesis", "will", "provide", "an", "opportunity", "to", "revive", "hematopoiesis", "as", "one", "of", "the", "most", "fascinating", "targets", "of", "research", "in", "developmental", "biology"]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0, 5, 6, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Epstein-Barr", "Virus", "and", "its", "glycoprotein-350", "upregulate", "IL-6", "in", "human", "B-lymphocytes", "via", "CD21", ",", "involving", "activation", "of", "NF-kappaB", "and", "different", "signaling", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Epstein-Barr", "virus", "(", "EBV", ")", "is", "a", "ubiquitous", "and", "highly", "immunotropic", "gamma", "herpesvirus", "that", "infects", "more", "than", "90", "%", "of", "humans", "worldwide", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Its", "pathogenicity", "leads", "to", "a", "number", "of", "diseases", "including", "tumors", "that", "result", "from", "EBV", "'s", "ability", "to", "readily", "transform", "B-lymphocytes", "and", ",", "to", "a", "lesser", "extent", ",", "epithelial", "cells", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["EBV", "utilizes", "CD21/CR2", "as", "its", "receptor", "on", "B", "cells", "to", "initiate", "the", "infection", "process", "."]} {"tags": [0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["EBV", "binds", "to", "CR2", "through", "its", "major", "envelope", "glycoprotein-350", "(", "gp350", ")", "and", "is", "also", "a", "remarkable", "immunomodulating", "agent", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["We", "had", "previously", "shown", "that", "EBV", "is", "capable", "of", "modulating", "the", "synthesis", "of", "a", "number", "of", "cytokines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 9, 10, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["We", "now", "show", "that", "while", "both", "purified", "recombinant", "gp350", "(", "rgp350", ")", "and", "EBV", "upregulate", "IL-6", "mRNA", "synthesis", "in", "B", "cells", ",", "EBV-induced", "IL-6", "gene", "activation", "occurs", "for", "a", "significantly", "longer", "period", "of", "time", "(", "i.e.", "12", "hours", "for", "EBV", "as", "compared", "to", "6", "hours", "for", "rgp350", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "the", "half-life", "of", "EBV-induced", "IL-6", "mRNA", "was", "also", "significantly", "longer", "(", "10", "hours", ")", "than", "that", "of", "mRNA", "induced", "by", "rgp350", "(", "about", "6", "hours", ")", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Both", "EBV", "and", "gp350", "enhance", "the", "binding", "of", "the", "NF-kappaB", "transcription", "factor", ",", "as", "determined", "by", "band-shift", "and", "augment", "NF-kappaB", "-mediated", "activation", "of", "a", "CAT", "reporter", "plasmid", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "we", "demonstrate", "that", "while", "the", "activation", "of", "IL-6", "gene", "expression", "by", "gp350", "is", "mediated", "primarily", "by", "the", "protein", "kinase", "C", "pathway", ",", "EBV", "can", "mediate", "its", "effects", "through", "multiple", "signaling", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["To", "our", "knowledge", "this", "is", "the", "first", "report", "showing", "that", "the", "binding", "of", "a", "herpesvirus", "envelope", "glycoprotein", "to", "CR2", "on", "human", "B", "cells", "results", "in", "the", "activation", "of", "the", "NF-kappaB", "transcription", "factor", "leading", "to", "the", "upregulation", "of", "IL-6", "gene", "expression", "in", "these", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Copyright", "2001", "Academic", "Press", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Gene-", "and", "tissue-specificity", "of", "mutation", "in", "Big", "Blue", "rats", "treated", "with", "the", "hepatocarcinogen", "N-hydroxy-2-acetylaminofluorene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 5, 6, 6, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 5, 6, 0], "tokens": ["In", "a", "previous", "study", ",", "we", "found", "that", "treating", "transgenic", "Big", "Blue", "rats", "with", "the", "hepatocarcinogen", "N-hydroxy-2-acetylaminofluorene", "(", "N-OH-AAF", ")", "produced", "the", "same", "major", "DNA", "adduct", "in", "the", "target", "liver", "and", "the", "nontarget", "spleen", "lymphocytes", "and", "bone", "marrow", "cells", ",", "induced", "lacI", "mutants", "in", "the", "liver", ",", "and", "induced", "much", "lower", "frequencies", "of", "l", "acI", "and", "hprt", "mutants", "in", "spleen", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "the", "present", "study", ",", "sequence", "analysis", "was", "conducted", "on", "lacI", "DNA", "and", "hprt", "cDNA", "from", "the", "mutants", ",", "to", "determine", "the", "mutational", "specificity", "of", "N-OH-AAF", "in", "the", "rat", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["All", "the", "mutation", "spectra", "from", "N-OH-AAF-treated", "rats", "differed", "significantly", "from", "corresponding", "mutation", "profiles", "from", "untreated", "animals", "(", "P", "=", "0.02", "to", "P", "<", "0.0001", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "there", "were", "similarities", "among", "the", "mutational", "patterns", "derived", "from", "N-OH-AAF-treated", "rats", "(", "e.g.", ",", "G", ":", "C", "--", ">", "T", ":", "A", "transversion", "was", "the", "most", "common", "mutation", "in", "all", "mutation", "sets", ")", ",", "there", "were", "significant", "differences", "in", "the", "patterns", "of", "basepair", "substitution", "and", "frameshift", "mutation", "between", "the", "liver", "and", "spleen", "lymphocyte", "lacI", "mutants", "(", "P", "=", "0.02", ")", "and", "between", "the", "spleen", "lymphocyte", "lacI", "and", "hprt", "mutants", "(", "P", "=", "0.04", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Also", ",", "multiplex", "PCR", "analysis", "of", "genomic", "DNA", "from", "the", "hprt", "mutants", "indicated", "that", "12", "%", "of", "mutants", "from", "treated", "rats", "had", "major", "deletions", "in", "the", "hprt", "gene", ";", "no", "corresponding", "incidence", "of", "large", "deletions", "was", "evident", "among", "lacI", "mutations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["All", "the", "mutation", "profiles", "reflect", "the", "general", "mutational", "specificity", "of", "the", "major", "DNA", "adduct", "formed", "by", "N-OH-AAF", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "differences", "between", "N-OH-AAF", "mutation", "in", "the", "endogenous", "gene", "and", "transgene", "can", "be", "partially", "explained", "by", "the", "structures", "of", "the", "two", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "tissue-specificity", "of", "the", "mutation", "spectra", "may", "contribute", "to", "targeting", "tumor", "formation", "to", "the", "liver", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Environ.", "Mol.", "Mutagen.", "37", ":", "203-214", ",", "2001", "."]} {"tags": [0, 0, 0, 0, 0, 0], "tokens": ["Published", "2001", "Wiley-Liss", ",", "Inc", "."]} {"tags": [3, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Caspase", "-dependent", "cleavage", "of", "the", "hematopoietic", "specific", "adaptor", "protein", "Gads", "alters", "signalling", "from", "the", "T", "cell", "receptor", "."]} {"tags": [3, 0, 0, 3, 4, 4, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Gads", "is", "a", "SH2", "and", "SH3", "domain", "-containing", ",", "hematopoietic-specific", "adaptor", "protein", "that", "functions", "in", "signalling", "from", "the", "T", "cell", "receptor", "."]} {"tags": [3, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Gads", "acts", "by", "linking", "SLP-76", ",", "bound", "by", "the", "carboxy-terminal", "Gads", "SH3", "domain", ",", "to", "tyrosine", "phosphorylated", "LAT", "which", "contains", "binding", "sites", "for", "the", "Gads", "SH2", "domain", "."]} {"tags": [3, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Gads", "is", "distinguished", "from", "Grb2", "and", "the", "closely", "related", "Grap", "protein", "by", "the", "presence", "of", "a", "120", "amino", "acid", "unique", "region", "between", "the", "SH2", "domain", "and", "the", "carboxy", "terminal", "SH3", "domain", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0], "tokens": ["Here", "we", "demonstrate", "that", "the", "unique", "region", "of", "Gads", "contains", "a", "capase", "cleavage", "site", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Induction", "of", "apoptosis", "in", "lymphocytes", "results", "in", "detectable", "Gads", "cleavage", "by", "60", "min", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Gads", "cleavage", "is", "blocked", "in", "vivo", "by", "treating", "cells", "with", "a", "caspase", "3", "inhibitor", "."]} {"tags": [0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "putative", "caspase", "3", "cleavage", "site", "was", "identified", "within", "the", "unique", "region", "and", "mutation", "of", "this", "site", "prevented", "Gads", "cleavage", "in", "vitro", ",", "and", "in", "vivo", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["The", "Gads", "cleavage", "products", "retained", "the", "predicted", "binding", "specificity", "for", "SLP-76", "and", "LAT", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Expression", "of", "the", "Gads", "cleavage", "products", "in", "Jurkat", "T", "cells", "inhibited", "NFAT", "activation", "following", "TCR", "cross", "linking", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["These", "findings", "indicate", "that", "cleavage", "of", "Gads", "in", "vivo", "could", "function", "to", "alter", "signalling", "downstream", "of", "the", "T", "cell", "receptor", "by", "disrupting", "cross", "talk", "between", "SLP-76", "and", "LAT", "."]} {"tags": [0, 0, 3, 0, 0, 1, 2, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Targeting", "of", "p300", "to", "the", "interleukin-2", "promoter", "via", "CREB-Rel", "cross-talk", "during", "mitogen", "and", "oncogenic", "molecular", "signaling", "in", "activated", "T-cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "report", ",", "we", "explore", "the", "mechanisms", "of", "targeting", "of", "p300", "to", "the", "interleukin-2", "(", "IL-2", ")", "promoter", "in", "response", "to", "mitogenic", "and", "oncogenic", "molecular", "signals", "."]} {"tags": [0, 0, 3, 0, 3, 4, 4, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Recruitment", "of", "p300", "by", "cAMP-responsive", "element-binding", "protein-Rel", "cross-talk", "at", "the", "composite", "CD28", "response", "element", "(", "CD28RE", ")", "-", "TRE", "element", "of", "the", "IL-2", "promoter", "is", "essential", "for", "promoter", "inducibility", "during", "T-cell", "activation", ",", "and", "CD28RE-TRE", "is", "the", "exclusive", "target", "of", "the", "human", "T-cell", "lymphotropic", "virus", "type", "I", "oncoprotein", "Tax", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 1, 2, 0, 0, 0, 1, 2, 0, 0], "tokens": ["The", "intrinsic", "histone", "acetyltransferase", "activity", "of", "p300", "is", "dispensable", "for", "activation", "of", "the", "IL-2", "promoter", ",", "and", "the", "N-terminal", "743", "residues", "contain", "the", "minimal", "structural", "requirements", "for", "synergistic", "transactivation", "of", "the", "CD28RE-TRE", ",", "the", "IL-2", "promoter", ",", "and", "endogenous", "IL-2", "gene", "expression", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 1, 2, 0], "tokens": ["Mutational", "analysis", "of", "p300", "reveals", "differential", "structural", "requirements", "for", "the", "N-terminal", "p300", "module", "by", "individual", "cis-elements", "within", "the", "IL-2", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["These", "findings", "provide", "evidence", "that", "p300", "assembles", "at", "the", "IL-2", "promoter", "to", "form", "an", "enhanceosome-like", "signal", "transduction", "target", "that", "is", "centrally", "integrated", "at", "the", "CD28RE-TRE", "element", "of", "the", "IL-2", "promoter", "through", "specific", "protein", "module-targeted", "associations", "in", "activated", "T-cells", "."]} {"tags": [0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Regulation", "of", "cytokine", "production", "in", "T-cell", "responses", "to", "inhalant", "allergen", ":", "GATA-3", "expression", "distinguishes", "between", "Th1-", "and", "Th2-polarized", "immunity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BACKGROUND", ":", "The", "precise", "nature", "of", "allergen-specific", "cytokine", "responses", "in", "atopics", "versus", "non-atopics", ",", "in", "particular", "the", "'Th1", "polarity", "'", "of", "responses", "in", "non-atopics", ",", "remains", "controversial", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "is", "due", "in", "part", "to", "the", "relative", "insensitivity", "of", "cytokine", "detection", "systems", ",", "and", "associated", "variations", "in", "kinetics", "of", "cytokine", "production", "and", "catabolism", "in", "in", "vitro", "culture", "systems", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["As", "an", "alternative", "to", "cytokine", "measurement", ",", "this", "study", "focuses", "on", "expression", "of", "the", "transcription", "factor", "GATA-3", "for", "analysis", "of", "allergen-specific", "Th", "cell", "responses", "."]} {"tags": [0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Cord", "blood", "mononuclear", "cells", "were", "Th1-", "or", "Th2-polarized", "by", "culture", "in", "IL-12-", "or", "IL-4-employing", "established", "methods", ";", "PBMC", "from", "house", "dust", "mite", "(", "HDM", ")", "-sensitive", "atopics", "and", "controls", "were", "stimulated", "overnight", "with", "HDM", ";", "cytokine", "production", "was", "measured", "by", "ELISA", "and", "GATA-3", "mRNA", "expression", "by", "PCR", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "Cytokine", "-driven", "Th2", "polarization", "of", "naive", "T", "cells", "is", "associated", "with", "marked", "upregulation", "of", "GATA-3", "expression", ",", "whereas", "a", "reciprocal", "expression", "pattern", "accompanies", "differentiation", "towards", "the", "Th1", "cytokine", "phenotype", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["In", "T", "cells", "from", "HDM", "skin", "prick", "test-positive", "(", "HDM-SPT+/HDM-IgE+", ")", "volunteers", ",", "overnight", "stimulation", "results", "in", "marked", "upregulation", "of", "GATA-3", "expression", ",", "compared", "to", "an", "equally", "marked", "downregulation", "of", "expression", "in", "T", "cells", "from", "SPT-/IgE-", "subjects", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "subjects", "who", "are", "HDM-SPT+", "but", "IgE-", ",", "GATA-3", "expression", "levels", "remained", "relatively", "stable", "during", "culture", "with", "HDM", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSIONS", ":", "Upregulation", "of", "GATA-3", "expression", "in", "PBMC", "is", "a", "hallmark", "of", "the", "early", "phase", "of", "Th2", "recall", "responses", "to", "specific", "allergen", "in", "atopics", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "reciprocal", "expression", "pattern", "observed", "in", "HDM-specific", "recall", "responses", "of", "non-atopics", "provides", "independent", "confirmation", "of", "the", "presence", "of", "underlying", "Th1-like", "immunity", "in", "these", "subjects", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "parallel", "findings", "in", "neonatal", "T", "cells", "suggest", "that", "the", "same", "approach", "may", "be", "utilized", "for", "monitoring", "the", "progress", "of", "allergen-specific", "Th1/Th2", "memory", "development", "during", "early", "childhood", ",", "and", "hence", "in", "assessment", "of", "risk", "for", "future", "allergic", "disease", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Copyright", "2001", "S.", "Karger", "AG", ",", "Basel"]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "heat", "shock", "response", "reduces", "myelin", "oligodendrocyte", "glycoprotein", "-induced", "experimental", "autoimmune", "encephalomyelitis", "in", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 4, 4, 0, 3, 0, 0], "tokens": ["The", "stress", "response", "(", "SR", ")", "can", "block", "inflammatory", "gene", "expression", "by", "preventing", "activation", "of", "transcription", "factor", "nuclear", "factor-kappa", "B", "(", "NF-kappaB", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["As", "inflammatory", "gene", "expression", "contributes", "to", "the", "pathogenesis", "of", "demyelinating", "diseases", ",", "we", "tested", "the", "effects", "of", "the", "SR", "on", "the", "progression", "of", "the", "demyelinating", "disease", "experimental", "autoimmune", "encephalomyelitis", "(", "EAE", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 4, 0, 0, 0], "tokens": ["EAE", "was", "actively", "induced", "in", "C57BL/6", "mice", "using", "an", "encephalitogenic", "myelin", "oligodendrocyte", "glycoprotein", "(", "MOG", "(", "35-55", ")", ")", "peptide", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Whole", "body", "hyperthermia", "was", "used", "to", "induce", "a", "heat", "shock", "response", "(", "HSR", ")", "in", "immunized", "mice", "2", "days", "after", "the", "booster", "MOG", "(", "35-55", ")", "peptide", "injection", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "HSR", "reduced", "the", "incidence", "of", "EAE", "by", "70", "%", ",", "delayed", "disease", "onset", "by", "6", "days", ",", "and", "attenuated", "disease", "severity", "."]} {"tags": [0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 5, 0], "tokens": ["The", "HSR", "attenuated", "leukocyte", "infiltration", "into", "CNS", "assessed", "by", "quantitation", "of", "perivascular", "infiltrates", ",", "and", "by", "reduced", "staining", "for", "CD4", "and", "CD25", "immunopositive", "T-cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T-cell", "activation", ",", "assessed", "by", "the", "production", "of", "interferon", "gamma", "(", "IFNgamma", ")", "in", "response", "to", "MOG", "(", "35-55", ")", ",", "was", "also", "decreased", "by", "the", "HSR", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "HSR", "reduced", "inflammatory", "gene", "expression", "in", "the", "brain", "that", "normally", "occurs", "during", "EAE", ",", "including", "the", "early", "increase", "in", "RANTES", "(", "regulated", "on", "activation", "of", "normal", "T-cell", "expressed", "and", "secreted", ")", "expression", ",", "and", "the", "later", "expression", "of", "the", "inducible", "form", "of", "nitric", "oxide", "synthase", "."]} {"tags": [0, 0, 0, 0, 3, 4, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "early", "activation", "of", "transcription", "factor", "NF-kappaB", "was", "also", "blocked", "by", "the", "HSR", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "finding", "that", "the", "SR", "reduces", "inflammation", "in", "the", "brain", "and", "the", "clinical", "severity", "of", "EAE", "opens", "a", "novel", "therapeutic", "approach", "for", "prevention", "of", "autoimmune", "diseases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["In", "vivo", "detection", "of", "intracellular", "signaling", "pathways", "in", "developing", "thymocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Information", "regarding", "the", "intracellular", "signaling", "processes", "that", "occur", "during", "the", "development", "of", "T", "cells", "has", "largely", "been", "obtained", "with", "the", "use", "of", "transgenic", "mouse", "models", ",", "which", "although", "providing", "invaluable", "information", "are", "time", "consuming", "and", "costly", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["To", "this", "end", ",", "we", "have", "developed", "a", "novel", "system", "that", "facilitates", "the", "in", "vivo", "analysis", "of", "signal", "transduction", "pathways", "during", "T-lymphocyte", "development", "."]} {"tags": [0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 4, 0], "tokens": ["This", "approach", "uses", "reporter-plasmids", "for", "the", "detection", "of", "intracellular", "signals", "mediated", "by", "the", "mitogen-activated", "protein", "kinase", "or", "cyclic", "AMP-dependent", "protein", "kinase", "."]} {"tags": [1, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Reporter-plasmids", "are", "transfected", "into", "thymocytes", "in", "fetal", "thymic", "organ", "culture", "by", "accelerated", "DNA/particle", "bombardment", "(", "gene", "gun", ")", ",", "and", "the", "activation", "of", "a", "signaling", "pathway", "is", "determined", "in", "the", "form", "of", "a", "standard", "luciferase", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Importantly", ",", "this", "powerful", "technique", "preserves", "the", "structural", "integrity", "of", "the", "thymus", ",", "and", "will", "provide", "an", "invaluable", "tool", "to", "study", "how", "thymocytes", "respond", "to", "normal", "environmental", "stimuli", "encountered", "during", "differentiation", "within", "the", "thymic", "milieu", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Thus", ",", "this", "method", "allows", "for", "the", "monitoring", "of", "signals", "that", "occur", "in", "a", "biological", "time", "frame", ",", "such", "as", "during", "differentiation", ",", "and", "within", "the", "natural", "environment", "of", "differentiating", "cells", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0], "tokens": ["Core-binding", "factor", "beta", "(", "CBFbeta", ")", ",", "but", "not", "CBFbeta-smooth", "muscle", "myosin", "heavy", "chain", ",", "rescues", "definitive", "hematopoiesis", "in", "CBFbeta", "-deficient", "embryonic", "stem", "cells", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0], "tokens": ["Core-binding", "factor", "beta", "(", "CBFbeta", ")", "is", "the", "non-DNA-binding", "subunit", "of", "the", "heterodimeric", "CBFs", "."]} {"tags": [1, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Genes", "encoding", "CBFbeta", "(", "CBFB", ")", ",", "and", "one", "of", "the", "DNA-binding", "CBFalpha", "subunits", ",", "Runx1", "(", "also", "known", "as", "CBFalpha2", ",", "AML1", ",", "and", "PEBP2alphaB", ")", ",", "are", "required", "for", "normal", "hematopoiesis", "and", "are", "also", "frequent", "targets", "of", "chromosomal", "translocations", "in", "acute", "leukemias", "in", "humans", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Homozygous", "disruption", "of", "either", "the", "Runx1", "or", "Cbfb", "gene", "in", "mice", "results", "in", "embryonic", "lethality", "at", "midgestation", "due", "to", "hemorrhaging", "in", "the", "central", "nervous", "system", ",", "and", "severely", "impairs", "fetal", "liver", "hematopoiesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Results", "of", "this", "study", "show", "that", "Cbfb-deficient", "mouse", "embryonic", "stem", "(", "ES", ")", "cells", "can", "differentiate", "into", "primitive", "erythroid", "colonies", "in", "vitro", ",", "but", "are", "impaired", "in", "their", "ability", "to", "produce", "definitive", "erythroid", "and", "myeloid", "colonies", ",", "mimicking", "the", "in", "vivo", "defect", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0], "tokens": ["Definitive", "hematopoiesis", "is", "restored", "by", "ectopic", "expression", "of", "full-length", "Cbfb", "transgenes", ",", "as", "well", "as", "by", "a", "transgene", "encoding", "only", "the", "heterodimerization", "domain", "of", "CBFbeta", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 3, 4, 4, 4, 0, 0, 7, 8, 8, 0, 7, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["In", "contrast", ",", "the", "CBFbeta", "-", "smooth", "muscle", "myosin", "heavy", "chain", "(", "SMMHC", ")", "fusion", "protein", "generated", "by", "the", "inv", "(", "16", ")", "associated", "with", "acute", "myeloid", "leukemias", "(", "M4Eo", ")", "can", "not", "rescue", "definitive", "hematopoiesis", "by", "Cbfb-deficient", "ES", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0], "tokens": ["Sequences", "responsible", "for", "the", "inability", "of", "CBFbeta", "-SMMHC", "to", "rescue", "definitive", "hematopoiesis", "reside", "in", "the", "SMMHC", "portion", "of", "the", "fusion", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Results", "also", "show", "that", "the", "CBFbeta-SMMHC", "fusion", "protein", "transdominantly", "inhibits", "definitive", "hematopoiesis", ",", "but", "not", "to", "the", "same", "extent", "as", "homozygous", "loss", "of", "Runx1", "or", "Cbfb", "."]} {"tags": [3, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["CBFbeta-SMMHC", "preferentially", "inhibits", "the", "differentiation", "of", "myeloid", "lineage", "cells", ",", "while", "increasing", "the", "number", "of", "blastlike", "cells", "in", "culture", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 6, 6, 0], "tokens": ["The", "latency", "pattern", "of", "Epstein-Barr", "virus", "infection", "and", "viral", "IL-10", "expression", "in", "cutaneous", "natural", "killer/T-cell", "lymphomas", "."]} {"tags": [0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "nasal", "type", ",", "extranodal", "natural", "killer", "or", "T", "(", "NK/T", ")", "-cell", "lymphoma", "is", "usually", "associated", "with", "latent", "Epstein-Barr", "virus", "(", "EBV", ")", "infection", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "order", "to", "elucidate", "the", "EBV", "gene", "expression", "patterns", "in", "vivo", ",", "we", "examined", "eight", "patients", "with", "cutaneous", "EBV-related", "NK/T-cell", "lymphomas", ",", "including", "six", "patients", "with", "a", "NK-cell", "phenotype", "and", "two", "patients", "with", "a", "T-cell", "phenotype", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0], "tokens": ["The", "implication", "of", "EBV", "in", "the", "skin", "lesions", "was", "determined", "by", "the", "presence", "of", "EBV-DNA", ",", "EBV-encoded", "nuclear", "RNA", "(", "EBER", ")", "and", "a", "clonality", "of", "EBV-DNA", "fragments", "containing", "the", "terminal", "repeats", "."]} {"tags": [0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transcripts", "of", "EBV-encoded", "genes", "were", "screened", "by", "reverse", "transcription-", "polymerase", "chain", "reaction", "(", "RT-PCR", ")", ",", "and", "confirmed", "by", "Southern", "blot", "hybridization", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["The", "expression", "of", "EBV-related", "antigens", "was", "examined", "by", "immunostaining", "using", "paraffin-embedded", "tissue", "sections", "and", "cell", "pellets", "of", "EBV-positive", "cell", "lines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 1, 0, 0, 0, 0, 7, 8, 8, 0, 7, 0, 7, 0, 7, 0], "tokens": ["Our", "study", "demonstrated", "that", "all", "samples", "from", "the", "patients", "contained", "EBV", "nuclear", "antigen", "(", "EBNA", ")", "-1", "mRNA", "which", "was", "transcribed", "using", "the", "Q", "promoter", ",", "whereas", "both", "the", "Q", "promoter", "and", "another", "upstream", "promoter", "(", "Cp/Wp", ")", "were", "used", "in", "EBV-positive", "cell", "lines", ",", "B95.8", ",", "Raji", "and", "Jiyoye", "."]} {"tags": [9, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 9, 10, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["Latent", "membrane", "protein-1", "(", "LMP-1", ")", "mRNA", "was", "detected", "in", "seven", "of", "eight", "patients", "and", "all", "cell", "lines", ",", "whereas", "EBNA-2", "transcripts", "were", "found", "only", "in", "the", "cell", "lines", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Immunostaining", "showed", "no", "LMP-1", ",", "EBNA-2", "or", "ZEBRA", "antigens", "in", "the", "paraffin-embedded", "tissue", "sections", ",", "although", "they", "were", "positive", "in", "the", "cell", "line", "cells", "."]} {"tags": [9, 10, 10, 0, 9, 10, 0, 9, 10, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Latent", "BHRF1", "transcripts", "encoding", "bcl-2", "homologue", "and", "BCRF1", "transcripts", "encoding", "viral", "interleukin", "(", "vIL", ")", "-10", "were", "detected", "in", "one", "and", "two", "of", "eight", "patients", ",", "respectively", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "patient", "with", "NK-cell", "lymphoma", "expressing", "both", "transcripts", "died", "of", "rapid", "progression", "of", "the", "illness", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "results", "indicate", "that", "the", "restricted", "expression", "of", "the", "latency-associated", "EBV", "genes", "and", "the", "production", "of", "vIL-10", "and", "bcl-2", "homologue", "may", "favour", "tumour", "growth", ",", "evading", "the", "host", "immune", "surveillance", "."]} {"tags": [0, 0, 0, 0, 0, 0], "tokens": ["Copyright", "2001", "Cancer", "Research", "Campaign", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Oxidized", "alkyl", "phospholipids", "are", "specific", ",", "high", "affinity", "peroxisome", "proliferator-activated", "receptor", "gamma", "ligands", "and", "agonists", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Synthetic", "high", "affinity", "peroxisome", "proliferator-activated", "receptor", "(", "PPAR", ")", "agonists", "are", "known", ",", "but", "biologic", "ligands", "are", "of", "low", "affinity", "."]} {"tags": [3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Oxidized", "low", "density", "lipoprotein", "(", "oxLDL", ")", "is", "inflammatory", "and", "signals", "through", "PPARs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["We", "showed", ",", "by", "phospholipase", "A", "(", "1", ")", "digestion", ",", "that", "PPARgamma", "agonists", "in", "oxLDL", "arise", "from", "the", "small", "pool", "of", "alkyl", "phosphatidylcholines", "in", "LDL", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["We", "identified", "an", "abundant", "oxidatively", "fragmented", "alkyl", "phospholipid", "in", "oxLDL", ",", "hexadecyl", "azelaoyl", "phosphatidylcholine", "(", "azPC", ")", ",", "as", "a", "high", "affinity", "ligand", "and", "agonist", "for", "PPARgamma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["[", "(", "3", ")", "H", "]", "azPC", "bound", "recombinant", "PPARgamma", "with", "an", "affinity", "(", "K", "(", "d", ")", "(", "(", "app", ")", ")", "approximately", "40", "nm", ")", "that", "was", "equivalent", "to", "rosiglitazone", "(", "BRL49653", ")", ",", "and", "competition", "with", "rosiglitazone", "showed", "that", "binding", "occurred", "in", "the", "ligand-binding", "pocket", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["azPC", "induced", "PPRE", "reporter", "gene", "expression", ",", "as", "did", "rosiglitazone", ",", "with", "a", "half-maximal", "effect", "at", "100", "nm", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Overexpression", "of", "PPARalpha", "or", "PPARgamma", "revealed", "that", "azPC", "was", "a", "specific", "PPARgamma", "agonist", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0], "tokens": ["The", "scavenger", "receptor", "CD36", "is", "encoded", "by", "a", "PPRE-responsive", "gene", ",", "and", "azPC", "enhanced", "expression", "of", "CD36", "in", "primary", "human", "monocytes", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "found", "that", "anti-CD36", "inhibited", "azPC", "uptake", ",", "and", "it", "inhibited", "PPRE", "reporter", "induction", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Results", "with", "a", "small", "molecule", "phospholipid", "flippase", "mimetic", "suggest", "azPC", "acts", "intracellularly", "and", "that", "cellular", "azPC", "accumulation", "was", "efficient", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 1, 2], "tokens": ["Thus", ",", "certain", "alkyl", "phospholipid", "oxidation", "products", "in", "oxLDL", "are", "specific", ",", "high", "affinity", "extracellular", "ligands", "and", "agonists", "for", "PPARgamma", "that", "induce", "PPAR-responsive", "genes"]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 3, 4, 4, 0, 3, 4, 0, 3, 4, 0], "tokens": ["Regulation", "of", "the", "human", "MAT2B", "gene", "encoding", "the", "regulatory", "beta", "subunit", "of", "methionine", "adenosyltransferase", ",", "MAT", "II", "."]} {"tags": [3, 4, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Methionine", "adenosyltransferase", "(", "MAT", ")", "catalyzes", "the", "biosynthesis", "of", "S-adenosylmethionine", "(", "AdoMet", ")", ",", "a", "key", "molecule", "in", "transmethylation", "reactions", "and", "polyamine", "biosynthesis", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0], "tokens": ["The", "MAT", "II", "isozyme", "consists", "of", "a", "catalytic", "alpha2", "and", "a", "regulatory", "beta", "subunit", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Down-regulation", "of", "the", "MAT", "II", "beta", "subunit", "expression", "causes", "a", "6-10-fold", "increase", "in", "intracellular", "AdoMet", "levels", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0], "tokens": ["To", "understand", "the", "mechanism", "by", "which", "the", "beta", "subunit", "expression", "is", "regulated", ",", "we", "cloned", "the", "MAT2B", "gene", ",", "determined", "its", "organization", ",", "characterized", "its", "5'-flanking", "sequences", ",", "and", "elucidated", "the", "in", "vitro", "and", "in", "vivo", "regulation", "of", "its", "promoter", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Transcription", "of", "the", "MAT2B", "gene", "initiates", "at", "position", "-203", "relative", "to", "the", "translation", "start", "site", "."]} {"tags": [1, 2, 2, 0, 0, 1, 2, 0, 1, 2, 2, 2, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Promoter", "deletion", "analysis", "defined", "a", "minimal", "promoter", "between", "positions", "+52", "and", "+93", "base", "pairs", ",", "a", "GC-rich", "region", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0], "tokens": ["Inclusion", "of", "the", "sequences", "between", "-4", "and", "+52", "enhanced", "promoter", "activity", ";", "this", "was", "primarily", "because", "of", "an", "Sp1", "recognition", "site", "at", "+9/+15", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0], "tokens": ["The", "inclusion", "of", "sequences", "up", "to", "position", "-115", "provided", "full", "activity", ";", "this", "was", "attributed", "to", "a", "TATA", "at", "-32", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "Sp1", "site", "at", "position", "+9", "was", "key", "for", "the", "formation", "of", "protein.DNA", "complexes", "."]} {"tags": [0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mutation", "of", "both", "the", "Sp1", "site", "at", "+9", "and", "the", "TATA", "at", "-32", "reduced", "promoter", "activity", "to", "its", "minimal", "level", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Supershift", "assays", "showed", "no", "effect", "of", "the", "anti-Sp1", "antibody", "on", "complex", "formation", ",", "whereas", "the", "anti-Sp3", "antibody", "had", "a", "strong", "effect", "on", "protein.DNA", "complex", "formation", ",", "suggesting", "that", "Sp3", "is", "one", "of", "the", "main", "factors", "binding", "to", "this", "Sp1", "site", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Chromatin", "immunoprecipitation", "assays", "supported", "the", "involvement", "of", "both", "Sp1", "and", "Sp3", "in", "complexes", "formed", "on", "the", "MAT2B", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["The", "data", "show", "that", "the", "5'-untranslated", "sequences", "play", "an", "important", "role", "in", "regulating", "the", "MAT2B", "gene", "and", "identifies", "the", "Sp1", "site", "at", "+9", "as", "a", "potential", "target", "for", "modulating", "MAT2B", "expression", ",", "a", "process", "that", "can", "have", "a", "major", "effect", "on", "intracellular", "AdoMet", "levels", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0], "tokens": ["Molecular", "pathogenesis", "of", "influenza", "A", "virus", "infection", "and", "virus-induced", "regulation", "of", "cytokine", "gene", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Despite", "vaccines", "and", "antiviral", "substances", "influenza", "still", "causes", "significant", "morbidity", "and", "mortality", "world", "wide", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Better", "understanding", "of", "the", "molecular", "mechanisms", "of", "influenza", "virus", "replication", ",", "pathogenesis", "and", "host", "immune", "responses", "is", "required", "for", "the", "development", "of", "more", "efficient", "means", "of", "prevention", "and", "treatment", "of", "influenza", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Influenza", "A", "virus", ",", "which", "replicates", "in", "epithelial", "cells", "and", "leukocytes", ",", "regulates", "host", "cell", "transcriptional", "and", "translational", "systems", "and", "activates", ",", "as", "well", "as", "downregulates", "apoptotic", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["Influenza", "A", "virus", "infection", "results", "in", "the", "production", "of", "chemotactic", "(", "RANTES", ",", "MIP-1", "alpha", ",", "MCP-1", ",", "MCP-3", ",", "and", "IP-10", ")", ",", "pro-inflammatory", "(", "IL-1", "beta", ",", "IL-6", ",", "IL-18", ",", "and", "TNF-alpha", ")", ",", "and", "antiviral", "(", "IFN-alpha/beta", ")", "cytokines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 3, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Cytokine", "gene", "expression", "is", "associated", "with", "the", "activation", "of", "NF-kappa", "B", ",", "AP-1", ",", "STAT", "and", "IRF", "signal", "transducing", "molecules", "in", "influenza", "A", "virus-infected", "cells", "."]} {"tags": [0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", "of", "upregulating", "cytokine", "gene", "expression", ",", "influenza", "A", "virus", "infection", "activates", "caspase-1", "enzyme", ",", "which", "is", "involved", "in", "the", "proteolytic", "processing", "of", "proIL-1", "beta", "and", "proIL-18", "into", "their", "biologically", "active", "forms", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 2, 2, 2, 0], "tokens": ["Influenza", "A", "virus-induced", "IFN-alpha/beta", "is", "essential", "in", "host", "'s", "antiviral", "defence", "by", "activating", "the", "expression", "of", "antiviral", "Mx", ",", "PKR", "and", "oligoadenylate", "synthetase", "genes", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["IFN-alpha/beta", "also", "prolongs", "T", "cell", "survival", ",", "upregulates", "IL-12", "and", "IL-18", "receptor", "gene", "expression", "and", "together", "with", "IL-18", "stimulates", "NK", "and", "T", "cell", "IFN-gamma", "production", "and", "the", "development", "of", "Th1-type", "immune", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Comparison", "of", "hprt", "and", "lacI", "mutant", "frequency", "with", "DNA", "adduct", "formation", "in", "N-hydroxy-2-acetylaminofluorene-treated", "Big", "Blue", "rats", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["N-Hydroxy-2-acetylaminofluorene", "(", "N-OH-AAF", ")", "is", "the", "proximate", "carcinogenic", "metabolite", "of", "the", "powerful", "rat", "liver", "carcinogen", "2-acetylaminofluorene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", ",", "transgenic", "Big", "Blue", "(", "R", ")", "rats", "were", "used", "to", "examine", "the", "relationship", "between", "in", "vivo", "mutagenicity", "and", "DNA", "adduct", "formation", "by", "N-OH-AAF", "in", "the", "target", "liver", "compared", "with", "that", "in", "nontarget", "tissues", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Male", "rats", "were", "given", "one", ",", "two", ",", "or", "four", "doses", "of", "25", "mg", "N-OH-AAF/kg", "body", "weight", "by", "i.p.", "injection", "at", "4-day", "intervals", ",", "and", "groups", "of", "treated", "and", "control", "rats", "were", "euthanized", "up", "to", "10", "weeks", "after", "beginning", "the", "dosing", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["Mutant", "frequencies", "were", "measured", "in", "the", "spleen", "lymphocyte", "hprt", "gene", ",", "and", "lacI", "mutant", "frequencies", "were", "determined", "in", "the", "liver", "and", "spleen", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["At", "6", "weeks", "after", "beginning", "the", "dosing", ",", "the", "hprt", "mutant", "frequency", "in", "spleen", "lymphocytes", "from", "the", "four-dose", "group", "was", "16.5", "x", "10", "(", "-6", ")", "compared", "with", "3.2", "x", "10", "(", "-6", ")", "in", "control", "animals", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Also", "at", "6", "weeks", ",", "rats", "given", "one", ",", "two", ",", "or", "four", "doses", "of", "N-OH-AAF", "had", "lacI", "mutant", "frequencies", "in", "the", "liver", "of", "97.6", ",", "155.6", ",", "and", "406.8", "x", "10", "(", "-6", ")", ",", "respectively", ",", "compared", "with", "a", "control", "frequency", "of", "25.7", "x", "10", "(", "-6", ")", ";", "rats", "given", "four", "doses", "had", "lacI", "mutant", "frequencies", "in", "spleen", "lymphocytes", "of", "55.8", "x", "10", "(", "-6", ")", "compared", "with", "a", "control", "frequency", "of", "20.4", "x", "10", "(", "-6", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Additional", "rats", "were", "evaluated", "for", "DNA", "adduct", "formation", "in", "the", "liver", ",", "spleen", "lymphocytes", ",", "and", "bone", "marrow", "by", "(", "32", ")", "P-postlabeling", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Adduct", "analysis", "was", "conducted", "1", "day", "after", "one", ",", "two", ",", "and", "four", "treatments", "with", "N-OH-AAF", ",", "5", "days", "after", "one", "treatment", ",", "and", "9", "days", "after", "two", "treatments", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["N-", "(", "Deoxyguanosin-8-yl", ")", "-2-aminofluorene", "was", "the", "major", "DNA", "adduct", "identified", "in", "all", "the", "tissues", "examined", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Adduct", "concentrations", "increased", "with", "total", "dose", "to", "maximum", "values", "in", "samples", "taken", "1", "day", "after", "two", "doses", ",", "and", "remained", "essentially", "the", "same", "after", "four", "doses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "samples", "taken", "after", "four", "doses", ",", "adduct", "levels", "were", "103", ",", "28", ",", "and", "7", "fmol/microg", "of", "DNA", "in", "liver", ",", "spleen", "lymphocytes", ",", "and", "bone", "marrow", ",", "respectively", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "results", "indicate", "that", "the", "extent", "of", "both", "DNA", "adduct", "formation", "and", "mutant", "induction", "correlates", "with", "the", "organ", "specificity", "for", "N-OH-AAF", "carcinogenesis", "in", "the", "rat", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Environ.", "Mol.", "Mutagen.", "37", ":", "195-202", ",", "2001", "."]} {"tags": [0, 0, 0, 0, 0, 0], "tokens": ["Published", "2001", "Wiley-Liss", ",", "Inc", "."]} {"tags": [0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Expression", "of", "Mad1", "in", "T", "cells", "leads", "to", "reduced", "thymic", "cellularity", "and", "impaired", "mitogen-induced", "proliferation", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 6, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["To", "investigate", "Mad1", "function", "in", "vivo", ",", "transgenic", "mice", "were", "generated", "that", "express", "a", "Mad1", "transgene", "in", "T", "lineage", "cells", "under", "the", "control", "of", "the", "proximal", "lck", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0], "tokens": ["Thymus", "size", "in", "lck-Mad1", "transgenic", "mice", "is", "drastically", "reduced", "although", "representation", "of", "the", "various", "thymocyte", "sub", "populations", "appears", "normal", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "investigate", "more", "closely", "any", "effects", "of", "Mad1", "expression", "on", "thymocytes", ",", "we", "examined", "thymic", "selection", "using", "MHC", "class", "I-restricted", "H-Y-TCR", "transgenic", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mad1", "expression", "in", "vivo", "reduces", "the", "efficiency", "of", "positive", "selection", "."]} {"tags": [0, 0, 5, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Furthermore", ",", "thymocytes", "and", "splenic", "T", "cells", "from", "lck-Mad1", "transgenic", "mice", "display", "a", "profound", "proliferative", "defect", "in", "response", "to", "activation", "with", "either", "PMA/Ionomycin", "or", "immobilized", "anti-CD3/CD28", "antibody", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["This", "proliferative", "defect", "is", "not", "reversed", "by", "addition", "of", "exogenous", "IL-2", "and", "is", "p53-independent", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["The", "growth", "inhibition", "caused", "by", "Mad1", "is", "overcome", "by", "expression", "of", "active", "c-Myc", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 5, 6, 6, 0, 5, 6, 6, 6, 0], "tokens": ["Differential", "requirement", "for", "the", "transcription", "factor", "PU.1", "in", "the", "generation", "of", "natural", "killer", "cells", "versus", "B", "and", "T", "cells", "."]} {"tags": [3, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["PU.1", "is", "a", "member", "of", "the", "Ets", "family", "of", "transcription", "factors", "required", "for", "the", "development", "of", "various", "lymphoid", "and", "myeloid", "cell", "lineages", ",", "but", "its", "role", "in", "natural", "killer", "(", "NK", ")", "cell", "development", "is", "not", "known", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 6, 6, 6, 6, 6, 0, 0, 0, 5, 6, 0, 0, 5, 6, 6, 6, 0], "tokens": ["The", "study", "shows", "that", "PU.1", "is", "expressed", "in", "NK", "cells", "and", "that", ",", "on", "cell", "transfer", "into", "alymphoid", "Rag2/gammac", "(", "-/-", ")", "mice", ",", "hematopoietic", "progenitors", "of", "PU.1", "(", "-/-", ")", "fetal", "liver", "cells", "could", "generate", "functional", "NK", "cells", "but", "not", "B", "or", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Nevertheless", ",", "the", "numbers", "of", "bone", "marrow", "NK", "cell", "precursors", "and", "splenic", "mature", "NK", "cells", "were", "reduced", "compared", "to", "controls", "."]} {"tags": [0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "PU.1", "(", "-/-", ")", "NK", "cells", "displayed", "reduced", "expression", "of", "the", "receptors", "for", "stem", "cell", "factor", "and", "interleukin", "(", "IL", ")", "-7", ",", "suggesting", "a", "nonredundant", "role", "for", "PU.1", "in", "regulating", "the", "expression", "of", "these", "cytokine", "receptor", "genes", "during", "NK", "cell", "development", "."]} {"tags": [5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["PU.1", "(", "-/-", ")", "NK", "cells", "also", "showed", "defective", "expression", "of", "inhibitory", "and", "activating", "members", "of", "the", "Ly49", "family", "and", "failed", "to", "proliferate", "in", "response", "to", "IL-2", "and", "IL-12", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "despite", "the", "less", "stringent", "requirement", "for", "PU.1", "in", "NK", "cell", "development", "compared", "to", "B", "and", "T", "cells", ",", "PU.1", "regulates", "NK", "cell", "differentiation", "and", "homeostasis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Benzene-extracted", "components", "are", "important", "for", "the", "major", "activity", "of", "diesel", "exhaust", "particles", ":", "effect", "on", "interleukin-8", "gene", "expression", "in", "human", "bronchial", "epithelial", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Epidemiologic", "and", "experimental", "studies", "suggest", "that", "diesel", "exhaust", "particles", "(", "DEPs", ")", "may", "be", "related", "to", "increasing", "respiratory", "mortality", "and", "morbidity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 0, 0, 0], "tokens": ["We", "have", "shown", "that", "DEPs", "augmented", "the", "production", "of", "inflammatory", "cytokines", "by", "human", "airway", "epithelial", "cells", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "better", "understand", "the", "mechanisms", "of", "their", "proinflammatory", "activities", ",", "we", "studied", "the", "effects", "of", "several", "components", "extracted", "from", "DEPs", "on", "interleukin", "(", "IL", ")", "-8", "expression", "in", "human", "bronchial", "epithelial", "cell", "line", "BEAS-2B", "and", "normal", "human", "airway", "epithelial", "cells", "obtained", "from", "very", "peripheral", "airways", "by", "an", "ultrathin", "bronchoscope", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0], "tokens": ["We", "used", "several", "agents", "active", "on", "signal", "transduction", "pathways", "in", "cytokine", "expression", ",", "such", "as", "the", "protein", "kinase", "C", "inhibitor", "staurosporin", ",", "antioxidant", "agents", "including", "N-acetyl", "cysteine", "(", "NAC", ")", "and", "pyrrolidine", "dithiocarbamate", "(", "PDTC", ")", ",", "and", "p38", "mitogen-activated", "protein", "kinase", "(", "MAPK", ")", "inhibitor", "SB203580", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Benzene-extracted", "components", "showed", "effects", "mimicking", "DEPs", "on", "IL-8", "gene", "expression", ",", "release", "of", "several", "cytokines", "(", "IL-8", ";", "granulocyte", "macrophage", "colony-stimulating", "factor", ";", "and", "regulated", "on", "activation", ",", "normal", "T", "cells", "expressed", "and", "secreted", ")", "and", "nuclear", "factor", "(", "NF", ")", "-kappa", "B", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["We", "also", "found", "that", "NAC", ",", "PDTC", ",", "and", "SB203580", "suppressed", "the", "activities", "of", "DEPs", "and", "their", "benzene", "extracts", ",", "suggesting", "the", "roles", "of", "oxidants-mediated", "NF-kappa", "B", "activation", "and", "p38MAPK", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Finally", ",", "benzo", "[", "a", "]", "pyrene", ",", "one", "of", "the", "important", "compounds", "included", "in", "the", "benzene", "component", ",", "replicated", "the", "activities", "shown", "by", "DEPs", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "nuclear", "receptor", "PPAR", "gamma", "is", "expressed", "by", "mouse", "T", "lymphocytes", "and", "PPAR", "gamma", "agonists", "induce", "apoptosis", "."]} {"tags": [3, 4, 4, 4, 4, 4, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Peroxisome", "proliferator-activated", "receptor", "(", "PPAR", ")", "-gamma", "is", "a", "nuclear", "hormone", "receptor", "that", "serves", "as", "a", "trans", "factor", "to", "regulate", "lipid", "metabolism", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Intense", "interest", "is", "focused", "on", "PPAR-gamma", "and", "its", "ligands", "owing", "to", "its", "putative", "role", "in", "adipocyte", "differentiation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Little", "is", "known", ",", "however", ",", "about", "the", "functions", "of", "PPAR-gamma", "in", "the", "immune", "system", ",", "especially", "in", "T", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 9, 0, 0, 0, 0], "tokens": ["We", "demonstrate", "that", "both", "naive", "and", "activated", "ovalbumin-specific", "T", "cells", "from", "DO11.10-transgenic", "mice", "express", "PPAR-gamma", "mRNA", "and", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["In", "order", "to", "determine", "the", "function", "of", "PPAR-gamma", ",", "T", "cells", "were", "stimulated", "with", "phorbol", "12-myristate", "13-acetate", "and", "ionomycin", "or", "antigen", "and", "antigen-presenting", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Simultaneous", "exposure", "to", "PPAR-gamma", "ligands", "(", "e.g.", "15-deoxy-Delta", "(", "12", ",", "14", ")", "-prostaglandin", "J", "(", "2", ")", ",", "troglitazone", ")", "showed", "drastic", "inhibition", "of", "proliferation", "and", "significant", "decreases", "in", "cell", "viability", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["The", "decrease", "in", "cell", "viability", "was", "due", "to", "apoptosis", "of", "the", "T", "lymphocytes", ",", "and", "occurred", "only", "when", "cells", "were", "treated", "with", "PPAR-gamma", ",", "and", "not", "PPAR-alpha", "agonists", ",", "revealing", "specificity", "of", "this", "response", "for", "PPAR-gamma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "observations", "suggest", "that", "PPAR-gamma", "agonists", "play", "an", "important", "role", "in", "regulating", "T", "cell-mediated", "immune", "responses", "by", "inducing", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T", "cell", "death", "via", "PPAR-gamma", "ligation", "may", "act", "as", "a", "potent", "anti-inflammatory", "signal", "in", "the", "immune", "system", ",", "and", "ligands", "could", "possibly", "be", "used", "to", "control", "disorders", "in", "which", "excessive", "inflammation", "occurs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Suppression", "of", "lung", "inflammation", "in", "rats", "by", "prevention", "of", "NF-kappaB", "activation", "in", "the", "liver", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Activation", "of", "NF-kappaB", "and", "production", "of", "NF-kappaB", "-dependent", "chemokines", "are", "thought", "to", "be", "involved", "in", "the", "pathogenesis", "of", "neutrophilic", "lung", "inflammation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Calpain-1", "inhibitor", "(", "CI-1", ")", "blocks", "activation", "of", "NF-kappaB", "by", "preventing", "proteolysis", "of", "the", "inhibitory", "protein", "IkappaB-alpha", "by", "the", "ubiquitin/proteasome", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "hypothesized", "that", "inhibition", "of", "proteasome", "function", "with", "CI-1", "would", "block", "NF-kappaB", "activation", "in", "vivo", "after", "intraperitoneal", "(", "i.p.", ")", "treatment", "with", "bacterial", "lipopolysaccharide", "(", "LPS", ")", ",", "and", "that", "NF-kappaB", "inhibition", "would", "be", "associated", "with", "suppression", "of", "chemokine", "gene", "expression", "and", "attenuation", "of", "neutrophilic", "alveolitis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "treated", "rats", "with", "a", "single", "i.p.", "injection", "of", "CI-1", "(", "10", "mg/kg", ")", "two", "hours", "prior", "to", "i.p.", "LPS", "(", "7", "mg/kg", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Treatment", "with", "Cl-1", "prevented", "degradation", "of", "IkappaB-alpha", "and", "activation", "of", "NF-kappaB", "in", "the", "liver", "in", "response", "to", "LPS", ";", "however", ",", "Cl-1", "treatment", "had", "no", "detected", "effect", "on", "NF-kappaB", "activation", "in", "lung", "tissue", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CI-1", "treatment", "prior", "to", "LPS", "resulted", "in", "40", "%", "lower", "MIP-2", "concentration", "in", "lung", "lavage", "fluid", "compared", "to", "rats", "treated", "with", "vehicle", "prior", "to", "LPS", "(", "502", "+/-", "112", "pg/ml", "vs.", "859", "+/-144", "pg/ml", ",", "P", "<", "0.05", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "CI-1", "treatment", "substantially", "inhibited", "LPS-induced", "neutrophilic", "alveolitis", "(", "2.7+", "/-", "1.2", "x", "10", "(", "5", ")", "vs.", "43.7", "+/-", "12.2", "x", "10", "(", "5", ")", "lung", "lavage", "neutrophils", ",", "P", "<", "0.01", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "indicate", "that", "NF-kappaB", "inhibition", "in", "the", "liver", "can", "alter", "lung", "inflammation", "induced", "by", "systemic", "LPS", "treatment", "and", "suggest", "that", "a", "liver-lung", "interaction", "contributes", "to", "the", "inflammatory", "response", "of", "the", "lung", "."]} {"tags": [3, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Notch1", "regulates", "maturation", "of", "CD4+", "and", "CD8+", "thymocytes", "by", "modulating", "TCR", "signal", "strength", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Notch", "signaling", "regulates", "cell", "fate", "decisions", "in", "multiple", "lineages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 5, 6, 6, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0], "tokens": ["We", "demonstrate", "in", "this", "report", "that", "retroviral", "expression", "of", "activated", "Notch1", "in", "mouse", "thymocytes", "abrogates", "differentiation", "of", "immature", "CD4+CD8+", "thymocytes", "into", "both", "CD4", "and", "CD8", "mature", "single-positive", "T", "cells", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["The", "ability", "of", "Notch1", "to", "inhibit", "T", "cell", "development", "was", "observed", "in", "vitro", "and", "in", "vivo", "with", "both", "normal", "and", "TCR", "transgenic", "thymocytes", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Notch1", "-mediated", "developmental", "arrest", "was", "dose", "dependent", "and", "was", "associated", "with", "impaired", "thymocyte", "responses", "to", "TCR", "stimulation", "."]} {"tags": [3, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Notch1", "also", "inhibited", "TCR-mediated", "signaling", "in", "Jurkat", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "indicate", "that", "constitutively", "active", "Notch1", "abrogates", "CD4+", "and", "CD8+", "maturation", "by", "interfering", "with", "TCR", "signal", "strength", "and", "provide", "an", "explanation", "for", "the", "physiological", "regulation", "of", "Notch", "expression", "during", "thymocyte", "development", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Expression", "of", "SART3", "antigen", "and", "induction", "of", "CTLs", "by", "SART3-derived", "peptides", "in", "breast", "cancer", "patients", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 5, 0, 0], "tokens": ["We", "recently", "reported", "the", "SART3", "tumour-rejection", "antigen", "as", "possessing", "tumour", "epitopes", "capable", "of", "inducing", "HLA-class", "I-restricted", "cytotoxic", "T", "lymphocytes", "(", "CTLs", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "study", "investigated", "expression", "of", "the", "SART3", "antigen", "in", "breast", "cancer", "to", "explore", "an", "appropriate", "molecule", "for", "use", "in", "specific", "immunotherapy", "of", "breast", "cancer", "patients", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "SART3", "antigen", "was", "detected", "in", "all", "of", "the", "breast", "cancer", "cell", "lines", "tested", ",", "30", "of", "40", "(", "75", "%", ")", "breast", "cancer", "tissue", "samples", ",", "and", "0", "of", "3", "non-tumourous", "breast", "tissue", "samples", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 5, 0, 0, 0, 5, 6, 6, 0, 0, 5, 6, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0], "tokens": ["SART3", "derived", "peptides", "at", "positions", "109-118", "and", "315-323", "induced", "HLA-A24", "restricted", "CTLs", "that", "reacted", "to", "breast", "cancer", "cells", "from", "the", "peripheral", "blood", "mononuclear", "cells", "(", "PBMCs", ")", "of", "breast", "cancer", "patients", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", ",", "the", "SART3", "antigen", "and", "its", "peptides", "could", "be", "an", "appropriate", "molecule", "for", "use", "in", "specific", "immunotherapy", "of", "the", "majority", "of", "HLA-A24-positive", "breast", "cancer", "patients"]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0], "tokens": ["Autostimulation", "of", "the", "Epstein-Barr", "virus", "BRLF1", "promoter", "is", "mediated", "through", "consensus", "Sp1", "and", "Sp3", "binding", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["As", "an", "essential", "step", "in", "the", "lytic", "cascade", ",", "the", "Rta", "homologues", "of", "gammaherpesviruses", "all", "activate", "their", "own", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 1, 0, 0, 0, 0, 0, 1, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0], "tokens": ["Consistent", "with", "this", "biologic", "function", ",", "the", "Epstein-Barr", "virus", "(", "EBV", ")", "Rta", "protein", "powerfully", "stimulates", "the", "promoter", "of", "its", "own", "gene", ",", "Rp", ",", "in", "EBV-positive", "B", "cells", "in", "transient-transfection", "reporter-based", "assays", "."]} {"tags": [0, 0, 0, 0, 0, 1, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["We", "analyzed", "the", "activity", "of", "RpCAT", "in", "response", "to", "Rta", "by", "deletional", "and", "site-directed", "mutagenesis", "."]} {"tags": [0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0], "tokens": ["Two", "cognate", "Sp1", "binding", "sites", "located", "at", "-279", "and", "-45", "relative", "to", "the", "transcriptional", "start", "site", "proved", "crucial", "for", "Rta-mediated", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 3, 0], "tokens": ["Previously", "described", "binding", "sites", "for", "the", "cellular", "transcription", "factor", "Zif268", "and", "the", "viral", "transactivator", "ZEBRA", "were", "found", "to", "be", "dispensable", "for", "activation", "of", "RpCAT", "by", "Rta", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 4, 0, 0, 1, 0, 0, 0], "tokens": ["Gel", "shift", "analysis", ",", "using", "extracts", "of", "B", "cells", "in", "latency", "or", "induced", "into", "the", "lytic", "cycle", ",", "identified", "Sp1", "and", "Sp3", "as", "the", "predominant", "cellular", "proteins", "bound", "to", "Rp", "near", "-45", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 1, 0, 0, 1, 2, 0], "tokens": ["During", "the", "lytic", "cycle", ",", "ZEBRA", "bound", "Rp", "near", "the", "Sp1/Sp3", "site", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["The", "binding", "of", "Sp1", "and", "Sp3", "to", "Rp", "correlated", "with", "the", "reporter", "activities", "in", "the", "mutagenesis", "study", ",", "establishing", "a", "direct", "link", "between", "transcriptional", "activation", "of", "Rp", "by", "Rta", "and", "DNA", "binding", "by", "Sp1", "and/or", "Sp3", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "relative", "abundance", "or", "functional", "state", "of", "the", "cellular", "Sp1", "and", "Sp3", "transcription", "factors", "may", "be", "altered", "in", "response", "to", "stimuli", "that", "induce", "the", "BRLF1", "promoter", "and", "thereby", "contribute", "to", "the", "activation", "of", "the", "viral", "lytic", "cycle", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Gene", "transfer", "of", "antisense", "hypoxia", "inducible", "factor-1", "alpha", "enhances", "the", "therapeutic", "efficacy", "of", "cancer", "immunotherapy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Solid", "tumors", "meet", "their", "demands", "for", "nascent", "blood", "vessels", "and", "increased", "glycolysis", ",", "to", "combat", "hypoxia", ",", "by", "activating", "multiple", "genes", "involved", "in", "angiogenesis", "and", "glucose", "metabolism", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Hypoxia", "inducible", "factor-1", "(", "HIF-1", ")", "is", "a", "constitutively", "expressed", "basic", "helix-loop-helix", "transcription", "factor", ",", "formed", "by", "the", "assembly", "of", "HIF-1alpha", "and", "HIF-1beta", "(", "Arnt", ")", ",", "that", "is", "stablized", "in", "response", "to", "hypoxia", ",", "and", "rapidly", "degraded", "under", "normoxic", "conditions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "activates", "the", "transcription", "of", "genes", "important", "for", "maintaining", "oxygen", "homeostasis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", ",", "we", "demonstrate", "that", "engineered", "down-regulation", "of", "HIF-1alpha", "by", "intratumoral", "gene", "transfer", "of", "an", "antisense", "HIF-1alpha", "plasmid", "leads", "to", "the", "down-regulation", "of", "VEGF", ",", "and", "decreased", "tumor", "microvessel", "density", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Antisense", "HIF-1alpha", "monotherapy", "resulted", "in", "the", "complete", "and", "permanent", "rejection", "of", "small", "(", "0.1", "cm", "in", "diameter", ")", "EL-4", "tumors", ",", "which", "is", "unusual", "for", "an", "anti-angiogenic", "agent", "where", "transient", "suppression", "of", "tumor", "growth", "is", "the", "norm", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "induced", "NK", "cell", "-dependent", "rejection", "of", "tumors", ",", "but", "failed", "to", "stimulate", "systemic", "T", "cell", "-mediated", "anti-tumor", "immunity", ",", "and", "synergized", "with", "B7-1-mediated", "immunotherapy", "to", "cause", "the", "NK", "cell", "and", "CD8", "T", "cell", "-dependent", "rejection", "of", "larger", "EL-4", "tumors", "(", "0.4", "cm", "in", "diameter", ")", "that", "were", "refractory", "to", "monotherapies", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mice", "cured", "of", "their", "tumors", "by", "combination", "therapy", "resisted", "a", "rechallenge", "with", "parental", "tumor", "cells", ",", "indicating", "systemic", "antitumor", "immunity", "had", "been", "achieved", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "summary", ",", "whilst", "intensive", "investigations", "are", "in", "progress", "to", "target", "the", "many", "HIF-1", "effectors", ",", "the", "results", "herein", "indicate", "that", "blocking", "hypoxia-inducible", "pathways", "and", "enhancing", "NK-mediated", "antitumor", "immunity", "by", "targeting", "HIF-1", "itself", "may", "be", "advantageous", ",", "especially", "when", "combined", "with", "cancer", "immunotherapy", "."]} {"tags": [1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Runx2", ":", "a", "novel", "oncogenic", "effector", "revealed", "by", "in", "vivo", "complementation", "and", "retroviral", "tagging", "."]} {"tags": [0, 1, 0, 1, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "Runx2", "(", "Cbfa1", ",", "Pebp2alphaA", ",", "Aml3", ")", "gene", "was", "previously", "identified", "as", "a", "frequent", "target", "for", "transcriptional", "activation", "by", "proviral", "insertion", "in", "T-cell", "lymphomas", "of", "CD2-MYC", "transgenic", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["We", "have", "recently", "shown", "that", "over-expression", "of", "the", "full-length", ",", "most", "highly", "expressed", "Runx2", "isoform", "in", "the", "thymus", "perturbs", "T-cell", "development", ",", "leads", "to", "development", "of", "spontaneous", "lymphomas", "at", "low", "frequency", "and", "is", "strongly", "synergistic", "with", "Myc", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 1, 2, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["To", "gain", "further", "insight", "into", "the", "relationship", "of", "Runx2", "to", "other", "lymphomagenic", "pathways", ",", "we", "tested", "the", "effect", "of", "combining", "the", "CD2-Runx2", "transgene", "either", "with", "a", "Pim1", "transgene", "(", "E", "(", "mu", ")", "-Pim1", ")", "or", "with", "the", "p53", "null", "genotype", ",", "as", "each", "of", "these", "displays", "independent", "synergy", "with", "Myc", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "both", "cases", "we", "observed", "synergistic", "tumour", "development", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "Runx2", "appeared", "to", "have", "a", "dominant", "effect", "on", "the", "tumour", "phenotype", "in", "each", "case", ",", "with", "most", "tumours", "conforming", "to", "the", "CD3", "(", "+", ")", ",", "CD8", "(", "+", ")", ",", "CD4", "(", "+/-", ")", "phenotype", "seen", "in", "CD2-Runx2", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Neonatal", "infection", "of", "CD2-Runx2", "mice", "with", "Moloney", "murine", "leukaemia", "virus", "(", "Moloney", "MLV", ")", "also", "led", "to", "a", "dramatic", "acceleration", "of", "tumour", "onset", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0], "tokens": ["Analysis", "of", "known", "Moloney", "MLV", "target", "genes", "in", "these", "lymphomas", "showed", "a", "high", "frequency", "of", "rearrangement", "at", "c-Myc", "or", "N-Myc", "(", "82", "%", ")", ",", "and", "a", "significant", "number", "at", "Pim1", "or", "Pim2", "(", "23", "%", ")", ",", "and", "at", "Pal1/Gfi1", "(", "18", "%", ")", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "indicate", "that", "Runx2", "makes", "a", "distinct", "contribution", "to", "T-cell", "lymphoma", "development", "which", "does", "not", "coincide", "with", "any", "of", "the", "oncogene", "complementation", "groups", "previously", "identified", "by", "retroviral", "tagging", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0, 5, 6, 0], "tokens": ["The", "involvement", "of", "TNF-alpha", "-related", "apoptosis-inducing", "ligand", "in", "the", "enhanced", "cytotoxicity", "of", "IFN-beta", "-stimulated", "human", "dendritic", "cells", "to", "tumor", "cells", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 5, 6, 0], "tokens": ["TNF-alpha", "-related", "apoptosis-inducing", "ligand", "(", "TRAIL", ")", "is", "characterized", "by", "its", "preferential", "induction", "of", "apoptosis", "of", "tumor", "cells", "but", "not", "normal", "cells", "."]} {"tags": [5, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Dendritic", "cells", "(", "DCs", ")", ",", "besides", "their", "role", "as", "APCs", ",", "now", "have", "been", "demonstrated", "to", "exert", "cytotoxicity", "or", "cytostasis", "on", "some", "tumor", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 5, 0, 5, 0, 0, 5, 6, 6, 6, 6, 6, 0, 5, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Here", ",", "we", "report", "that", "both", "human", "CD34", "(", "+", ")", "stem", "cell", "-derived", "DCs", "(", "CD34DCs", ")", "and", "human", "CD14", "(", "+", ")", "monocyte", "-derived", "DCs", "(", "MoDCs", ")", "express", "TRAIL", "and", "exhibit", "cytotoxicity", "to", "some", "types", "of", "tumor", "cells", "partially", "through", "TRAIL", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Moderate", "expression", "of", "TRAIL", "appeared", "on", "CD34DCs", "from", "the", "8th", "day", "of", "culture", "and", "was", "also", "seen", "on", "freshly", "isolated", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "level", "of", "TRAIL", "expression", "remained", "constant", "until", "DC", "maturation", "."]} {"tags": [0, 0, 0, 5, 6, 0, 5, 0, 0, 0, 0, 3, 0, 0], "tokens": ["TRAIL", "expression", "on", "immature", "CD34DCs", "or", "MoDCs", "was", "greatly", "up-regulated", "after", "IFN-beta", "stimulation", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 0, 5, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "IFN-beta", "could", "strikingly", "enhance", "the", "ability", "of", "CD34DCs", "or", "MoDCs", "to", "kill", "TRAIL-sensitive", "tumor", "cells", ",", "but", "LPS", "did", "not", "have", "such", "an", "effect", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 5, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 3, 0, 5, 0, 0, 0, 0], "tokens": ["The", "up-regulation", "of", "TRAIL", "on", "IFN-beta", "-stimulated", "DCs", "partially", "contributed", "to", "the", "increased", "cytotoxicity", "of", "DCS", ":", "Pretreatment", "of", "TRAIL-sensitive", "tumor", "cells", "with", "caspase-3", "inhibitor", "could", "significantly", "increase", "their", "resistance", "to", "the", "cytotoxicity", "of", "IFN-beta", "-stimulated", "DCS", ":", "In", "contrast", ",", "NF-kappaB", "inhibitor", "could", "significantly", "increase", "the", "sensitivity", "of", "tumor", "cells", "to", "the", "killing", "by", "nonstimulated", "or", "LPS-stimulated", "DCS", ":", "Our", "studies", "demonstrate", "that", "IFN-beta", "-stimulated", "DCs", "are", "functionally", "cytotoxic", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Thus", ",", "an", "innate", "mechanism", "of", "DC-mediated", "antitumor", "immunity", "might", "exist", "in", "vivo", "in", "which", "DCs", "act", "as", "effectors", "to", "directly", "kill", "tumor", "cells", "partially", "via", "TRAIL", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 5, 6, 6, 6, 6, 6, 0], "tokens": ["Subsequently", ",", "DCs", "act", "as", "APCs", "involved", "in", "the", "uptake", ",", "processing", ",", "and", "presentation", "of", "apoptotic", "tumor", "Ags", "to", "cross-prime", "CD8", "(", "+", ")", "CTL", "cells", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tumor", "cells", "regulate", "the", "lytic", "activity", "of", "tumor-specific", "cytotoxic", "t", "lymphocytes", "by", "modulating", "the", "inhibitory", "natural", "killer", "receptor", "function", "."]} {"tags": [5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tumor-infiltrating", "p58+", "T", "cells", "from", "a", "renal", "tumor", "were", "specifically", "expanded", "in", "response", "to", "tumor", "cell", "stimulation", "and", "cloned", "."]} {"tags": [0, 5, 6, 6, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "p58+", "T", "cells", "were", "found", "to", "express", "a", "memory", "phenotype", "and", "corresponded", "to", "clonal", "TCRBV3", "T-cell", "expansion", "."]} {"tags": [0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["Functionally", ",", "p58", "(", "+", ")", "CTLs", "displayed", "a", "low", "lytic", "activity", "for", "HLA-A2", "tumor", "and", "normal", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 0], "tokens": ["However", ",", "this", "lytic", "activity", "was", "significantly", "increased", "after", "blockade", "of", "p58", "with", "specific", "monoclonal", "antibodies", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Interestingly", ",", "we", "demonstrated", "that", "stimulation", "by", "tumor", "cells", "was", "required", "to", "trigger", "the", "inhibitory", "effect", "of", "p58", "on", "the", "lytic", "activity", "of", "antigen-specific", "CTLs", "and", "that", "stimulation", "of", "the", "inhibitory", "function", "of", "p58", "by", "tumor", "cells", "correlated", "with", "an", "inhibition", "of", "nuclear", "factor-kappaB", "activation", "in", "p58+", "tumor-specific", "CTLS", "."]} {"tags": [3, 4, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 3, 4, 4, 4, 0, 5, 6, 6, 0], "tokens": ["T-cell", "factor-1", "expression", "during", "human", "natural", "killer", "cell", "development", "and", "in", "circulating", "CD56", "(", "+", ")", "bright", "natural", "killer", "cells", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 6, 6, 0], "tokens": ["Transcription", "factors", "are", "essential", "to", "govern", "differentiation", "along", "the", "lymphoid", "lineage", "from", "uncommitted", "hematopoietic", "stem", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "many", "of", "these", "transcription", "factors", "have", "putative", "roles", "based", "on", "murine", "knockout", "experiments", ",", "their", "function", "in", "human", "lymphoid", "development", "is", "less", "known", "and", "was", "studied", "further", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0], "tokens": ["Transcription", "factor", "expression", "in", "fresh", "and", "cultured", "adult", "human", "bone", "marrow", "and", "umbilical", "cord", "blood", "progenitors", "was", "evaluated", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 0, 3, 0, 0, 3, 0], "tokens": ["We", "found", "that", "fresh", "CD34", "(", "+", ")", "Lin", "(", "-", ")", "cells", "that", "are", "human", "leukocyte", "antigen", "(", "HLA", ")", "-DR", "(", "-", ")", "or", "CD38", "(", "-", ")", "constitutively", "express", "GATA-3", "but", "not", "T-cell", "factor-1", "(", "TCF-1", ")", "or", "Id-3", "."]} {"tags": [0, 0, 0, 7, 8, 8, 8, 8, 8, 0, 0, 3, 0, 0, 0, 0, 9, 10, 0], "tokens": ["Culture", "with", "the", "murine", "fetal", "liver", "cell", "line", "AFT024", "and", "defined", "cytokines", "was", "capable", "of", "inducing", "TCF-1", "mRNA", "."]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "no", "T-cell", "receptor", "gene", "rearrangement", "was", "identified", "in", "cultured", "progeny", "."]} {"tags": [3, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Id-3", ",", "a", "basic", "helix", "loop", "helix", "factor", "with", "dominant", "negative", "function", "for", "T-cell", "differentiation", "transcription", "factors", ",", "also", "was", "upregulated", "and", "may", "explain", "unsuccessful", "T-cell", "maturation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 5, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["To", "better", "understand", "the", "developmental", "link", "between", "natural", "killer", "(", "NK", ")", "cells", "derived", "from", "progenitors", ",", "we", "studied", "NK", "cell", "subsets", "circulating", "in", "blood", "."]} {"tags": [5, 6, 6, 6, 0, 0, 0, 5, 6, 6, 6, 0, 5, 6, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CD56", "(", "+bright", ")", ",", "but", "not", "CD56", "(", "+dim", ")", ",", "NK", "cells", "constitutively", "express", "TCF-1", "by", "reverse", "transcriptase", "polymerase", "chain", "reaction", "and", "Western", "blot", "analysis", "."]} {"tags": [0, 3, 4, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "TCF-1", "isoform", "found", "in", "CD56", "(", "+bright", ")", "cells", ",", "which", "express", "lectin", "but", "not", "immunoglobulin", "class", "I", "recognizing", "inhibitory", "receptors", ",", "was", "identical", "to", "that", "induced", "in", "NK", "cell", "differentiation", "culture", "and", "was", "distinctly", "different", "from", "isoforms", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 1, 2, 2, 2, 2, 0, 3, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["These", "results", "suggest", "that", "TCF-1", "does", "not", "target", "human", "killer", "immunoglobulin", "receptor", "genes", ",", "TCF-1", "is", "uniquely", "expressed", "in", "circulating", "CD56", "(", "+bright", ")", "NK", "cells", ",", "and", "specific", "TCF-1", "isoforms", "may", "play", "an", "important", "role", "in", "regulating", "NK", "differentiation", "from", "a", "common", "NK/T-cell", "progenitor", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Transcriptional", "activation", "by", "a", "matrix", "associating", "region-binding", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["contextual", "requirements", "for", "the", "function", "of", "bright", "."]} {"tags": [3, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 5, 6, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 2, 0, 0], "tokens": ["Bright", "(", "B", "cell", "regulator", "of", "IgH", "transcription", ")", "is", "a", "B", "cell", "-specific", ",", "matrix", "associating", "region-binding", "protein", "that", "transactivates", "gene", "expression", "from", "the", "IgH", "intronic", "enhancer", "(", "E", "mu", ")", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["We", "show", "here", "that", "Bright", "has", "multiple", "contextual", "requirements", "to", "function", "as", "a", "transcriptional", "activator", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Bright", "can", "not", "transactivate", "via", "out", "of", "context", ",", "concatenated", "binding", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Transactivation", "is", "maximal", "on", "integrated", "substrates", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Two", "of", "the", "three", "previously", "identified", "binding", "sites", "in", "E", "mu", "are", "required", "for", "full", "Bright", "transactivation", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "Bright", "DNA", "binding", "domain", "defined", "a", "new", "family", ",", "which", "includes", "SWI1", ",", "a", "component", "of", "the", "SWI.SNF", "complex", "shown", "to", "have", "high", "mobility", "group-like", "DNA", "binding", "characteristics", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0], "tokens": ["Similar", "to", "one", "group", "of", "high", "mobility", "group", "box", "proteins", ",", "Bright", "distorts", "E", "mu", "binding", "site", "-containing", "DNA", "on", "binding", ",", "supporting", "the", "concept", "that", "it", "mediates", "E", "mu", "remodeling", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transfection", "studies", "further", "implicate", "Bright", "in", "facilitating", "spatially", "separated", "promoter-enhancer", "interactions", "in", "both", "transient", "and", "stable", "assays", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0], "tokens": ["Finally", ",", "we", "show", "that", "overexpression", "of", "Bright", "leads", "to", "enhanced", "DNase", "I", "sensitivity", "of", "the", "endogenous", "E", "mu", "matrix", "associating", "regions", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 0, 0], "tokens": ["These", "data", "further", "suggest", "that", "Bright", "may", "contribute", "to", "increased", "gene", "expression", "by", "remodeling", "the", "immunoglobulin", "locus", "during", "B", "cell", "development", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Analysis", "of", "BCL-6", "mutations", "in", "classic", "Hodgkin", "disease", "of", "the", "B-", "and", "T-cell", "type", "."]} {"tags": [1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0], "tokens": ["BCL-6", "is", "essential", "for", "germinal", "center", "formation", "and", "thus", "for", "affinity", "maturation", "of", "immunoglobulin", "(", "Ig", ")", "genes", "by", "somatic", "mutations", "."]} {"tags": [0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "5'-noncoding", "region", "of", "the", "BCL-6", "gene", "is", "even", "a", "target", "for", "the", "mutation", "machinery", "."]} {"tags": [0, 0, 0, 1, 2, 0, 1, 2, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Translocations", "of", "the", "BCL-6", "gene", "to", "heterologous", "promoters", "and", "mutations", "of", "its", "5'-noncoding", "regulatory", "region", "were", "reported", "to", "be", "potential", "mechanisms", "for", "deregulating", "BCL-6", "expression", "and", "for", "playing", "a", "role", "in", "the", "genesis", "of", "non-Hodgkin", "lymphoma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "line", "with", "this", "hypothesis", "is", "the", "observation", "that", "B-cell", "lymphoma", "with", "somatic", "mutations", ",", "such", "as", "diffuse", "large", "B-cell", "lymphoma", "and", "follicular", "lymphoma", ",", "also", "carry", "BCL-6", "mutations", ",", "some", "of", "which", "are", "recurrently", "detectable", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Classic", "Hodgkin", "disease", "(", "cHD", ")", "is", "also", "derived", "from", "B", "cells", "with", "high", "loads", "of", "somatic", "mutations", "and", "thus", "a", "further", "candidate", "for", "BCL-6", "mutations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0], "tokens": ["To", "determine", "the", "presence", "and", "potential", "role", "of", "BCL-6", "mutations", "in", "cHD", ",", "the", "5'-noncoding", "BCL-6", "proportion", "of", "single", "Hodgkin", "and", "Reed-Sternberg", "(", "HRS", ")", "cells", "from", "6", "cases", "of", "cHD", "and", "6", "cases", "of", "HD-derived", "cell", "lines", "was", "analyzed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["All", "B-cell-derived", "HD", "cases", "and", "cell", "lines", "harbored", "BCL-6", "mutations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "both", "T-cell-derived", "HD", "cases", "and", "cell", "lines", "were", "devoid", "of", "BCL-6", "mutations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["With", "only", "one", "exception", ",", "there", "were", "no", "lymphoma-specific", "recurrent", "BCL-6", "mutations", "detected", ",", "and", "BCL-6", "protein", "was", "absent", "from", "the", "HRS", "cells", "of", "most", "cases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "conclusion", ",", "(", "1", ")", "somatic", "BCL-6", "mutations", "are", "restricted", "to", "cHD", "cases", "of", "B-cell", "origin", ",", "and", "(", "2", ")", "the", "BCL-6", "mutations", "represent", "mostly", "irrelevant", "somatic", "base", "substitutions", "without", "consequences", "for", "BCL-6", "protein", "expression", "and", "the", "pathogenesis", "of", "cHD", "."]} {"tags": [1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Gadd45gamma", "is", "dispensable", "for", "normal", "mouse", "development", "and", "T-cell", "proliferation", "."]} {"tags": [1, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 5, 6, 6, 0], "tokens": ["Gadd45gamma", ",", "a", "family", "member", "of", "the", "growth", "arrest", "and", "DNA", "damage-inducible", "gene", "family", "45", "(", "Gadd45", ")", ",", "is", "strongly", "induced", "by", "interleukin-2", "(", "IL-2", ")", "in", "peripheral", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["While", "in", "most", "tissues", "all", "Gadd45", "family", "members", "are", "expressed", ",", "Gadd45gamma", "is", "the", "only", "member", "that", "is", "induced", "by", "IL-2", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 3, 0, 3, 0, 3, 4, 4, 4, 4, 4, 0, 0], "tokens": ["Here", "we", "show", "that", "the", "IL-2", "-induced", "expression", "of", "Gadd45gamma", "is", "dependent", "on", "a", "signaling", "pathway", "mediated", "by", "the", "tyrosine", "kinase", "Jak3", "and", "the", "transcription", "factors", "Stat5a", "and", "Stat5b", "(", "signal", "transducer", "and", "activator", "of", "transcription", ")", "."]} {"tags": [0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Previous", "studies", "with", "ectopically", "overexpressed", "Gadd45gamma", "in", "various", "cell", "lines", "implicated", "its", "function", "in", "negative", "growth", "control", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0], "tokens": ["To", "analyze", "the", "physiological", "role", "of", "Gadd45gamma", "we", "used", "homologous", "recombination", "to", "generate", "mice", "lacking", "Gadd45gamma", "."]} {"tags": [1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Gadd45gamma", "-deficient", "mice", "develop", "normally", ",", "are", "indistinguishable", "from", "their", "littermates", ",", "and", "are", "fertile", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 5, 6, 0, 0, 0, 0, 3, 0], "tokens": ["Furthermore", ",", "hematopoiesis", "in", "mice", "lacking", "Gadd45gamma", "is", "not", "impaired", "and", "Gadd45gamma", "-deficient", "T", "lymphocytes", "show", "normal", "responses", "to", "IL-2", "."]} {"tags": [0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["These", "data", "demonstrate", "that", "Gadd45gamma", "is", "not", "essential", "for", "normal", "mouse", "development", "and", "hematopoiesis", ",", "possibly", "due", "to", "functional", "redundancy", "among", "the", "Gadd45", "family", "members", "."]} {"tags": [1, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Gadd45gamma", "is", "also", "dispensable", "for", "IL-2", "-induced", "T-cell", "proliferation", "."]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Decreased", "expression", "of", "c-myc", "family", "genes", "in", "thymuses", "from", "myasthenia", "gravis", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0], "tokens": ["The", "thymus", "is", "a", "critical", "organ", "for", "the", "elimination", "of", "autoreactive", "T", "cells", "by", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 1, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "studied", "the", "expression", "of", "apoptosis-associated", "genes", ",", "bcl-xL", ",", "bad", ",", "caspase-3", ",", "and", "c-myc", "family", "genes", "in", "myasthenia", "gravis", "(", "MG", ")", "thymuses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "observed", "that", "the", "mRNA", "levels", "of", "myc", "family", "genes", ",", "c-myc", "and", "max", ",", "were", "markedly", "reduced", "in", "MG", "thymuses", "."]} {"tags": [0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "indicate", "that", "c-myc", "-mediated", "signaling", "is", "abnormal", "in", "MG", "thymuses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 3, 0, 3, 0, 0, 3], "tokens": ["The", "levels", "of", "molecules", "whose", "expressions", "are", "associated", "with", "myc", ",", "such", "as", "STAM", ",", "prothymosin-alpha", ",", "and", "NFkappaB"]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["Immunohistochemical", "detection", "of", "interferon-gamma-producing", "cells", "in", "dermatophytosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Skin", "lesions", "of", "dermatophytosis", "are", "thought", "to", "be", "a", "result", "of", "a", "T", "cell", "-dependent", "inflammatory", "response", "that", "is", "mediated", "by", "various", "cytokines", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "examined", "whether", "IFN-gamma-positive", "cells", "(", "as", "expression", "of", "Th1", "response", ")", "were", "present", "in", "the", "skin", "lesions", "of", "dermatophytosis", "in", "situ", "by", "immunohistochemical", "techniques", "."]} {"tags": [0, 0, 5, 6, 6, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mixtures", "of", "CD4-positive", "T", "cells", "and", "CD8-positive", "T", "cells", "were", "found", "to", "be", "present", "in", "the", "dermal", "infiltrates", "of", "the", "lesions", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Considerable", "numbers", "of", "CD1a-positive", "cells", "were", "detected", "in", "the", "upper", "dermis", "and", "epidermis", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "marked", "accumulation", "of", "CD68-positive", "cells", "was", "found", "in", "the", "upper", "dermis", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["IFN-gamma-positive", "cells", "were", "present", "in", "the", "upper", "dermis", "of", "the", "lesions", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["The", "pattern", "of", "IFN-gamma", "staining", "appeared", "to", "be", "intracellular", "in", "mononuclear", "lymphoid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["The", "staining", "was", "considered", "to", "be", "highly", "specific", "because", "it", "could", "be", "completely", "blocked", "by", "preabsorption", "with", "recombinant", "IFN-gamma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["Our", "data", "support", "the", "hypothesis", "that", "the", "skin", "lesions", "of", "dermatophytosis", "may", "be", "associated", "with", "a", "Th1", "response", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Th1", "response", ",", "which", "is", "characterized", "by", "IFN-gamma", "release", ",", "is", "thought", "to", "be", "involved", "in", "the", "host", "defense", "against", "dermatophytes", "and", "to", "reflect", "cutaneous", "reaction", "in", "dermatophytosis", "."]} {"tags": [0, 0, 0, 3, 4, 0, 3, 0, 3, 0, 0, 0, 3, 0, 3, 3, 0, 0], "tokens": ["Regulation", "of", "the", "helix-loop-helix", "proteins", ",", "E2A", "and", "Id3", ",", "by", "the", "Ras", "-", "ERK", "MAPK", "cascade", "."]} {"tags": [0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Activation", "of", "mitogen-activated", "protein", "kinase", "(", "MAPK", ")", "pathways", "leads", "to", "cellular", "differentiation", "and/or", "proliferation", "in", "a", "wide", "variety", "of", "cell", "types", ",", "including", "developing", "thymocytes", "."]} {"tags": [0, 3, 4, 4, 4, 4, 4, 3, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "basic", "helix-loop-helix", "(", "bHLH", ")", "proteins", "E12", "and", "E47", "and", "an", "inhibitor", "HLH", "protein", ",", "Id3", ",", "play", "key", "roles", "in", "thymocyte", "differentiation", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "show", "here", "that", "E2A", "DNA", "binding", "is", "lowered", "in", "primary", "immature", "thymocytes", "consequent", "to", "T", "cell", "receptor", "(", "TCR", ")", "-mediated", "ligation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Whereas", "expression", "of", "E2A", "mRNA", "and", "protein", "are", "unaltered", ",", "Id3", "transcripts", "are", "rapidly", "induced", "upon", "signaling", "from", "the", "TCR", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0], "tokens": ["Activation", "of", "Id3", "transcription", "is", "regulated", "in", "a", "dose-dependent", "manner", "by", "the", "extracellular", "signal-regulated", "kinase", "(", "ERK", ")", "MAPK", "module", "."]} {"tags": [0, 0, 0, 0, 0, 3, 3, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["These", "observations", "directly", "connect", "the", "ERK", "MAPK", "cascade", "and", "HLH", "proteins", "in", "a", "linear", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Retinoic", "acid", "up-regulates", "myeloid", "ICAM-3", "expression", "and", "function", "in", "a", "cell-specific", "fashion", "--", "evidence", "for", "retinoid", "signaling", "pathways", "in", "the", "mast", "cell", "lineage", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 7, 8, 8, 8, 8, 0], "tokens": ["Investigation", "of", "mast", "cell", "responsiveness", "toward", "retinoic", "acid", "(", "RA", ")", "revealed", "selective", "promotion", "of", "ICAM-3", "expression", "in", "the", "human", "mast", "cell", "line", "HMC-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "process", "was", "dose-", "and", "time-dependent", "and", "detectable", "by", "flow", "cytometry", ",", "Western", "blot", "analysis", ",", "ELISA", ",", "and", "Northern", "blot", "analysis", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 5, 0, 0, 7, 0, 0, 0, 0, 7, 8, 0], "tokens": ["ICAM-3", "modulation", "was", "found", "to", "be", "cell-type", "dependent", ",", "detectable", "also", "for", "HL-60", "cells", "and", "monocytes", "but", "not", "U-937", "and", "only", "weakly", "for", "KU812", "cells", "."]} {"tags": [5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Terminally", "differentiated", "skin", "mast", "cells", "also", "failed", "to", "up-modulate", "their", "ICAM-3", ",", "suggesting", "the", "requirement", "for", "some", "degree", "of", "immaturity", "for", "the", "process", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["RA-mediated", "effects", "on", "ICAM-1", "expression", ",", "studied", "in", "parallel", ",", "were", "clearly", "distinct", "from", "those", "on", "ICAM-3", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Investigation", "of", "retinoid", "receptor", "expression", ",", "known", "to", "mediate", "intracellular", "RA", "signaling", ",", "revealed", "presence", "of", "RAR", "alpha", ",", "RAR", "gamma", ",", "RXR", "beta", ",", "and", "RXR", "gamma", "transcripts", "in", "all", "cell", "lines", "studied", ",", "and", "HMC-1", "cells", "were", "the", "only", "line", "lacking", "RXR", "alpha", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["RAR", "beta", ",", "not", "expressed", "at", "baseline", ",", "was", "induced", "by", "RA", "in", "a", "fashion", "obviously", "correlating", "with", "ICAM-3", "up-regulation", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Increased", "ICAM-3", "expression", "was", "of", "functional", "significance", ",", "such", "that", "processes", "stimulated", "or", "co-stimulated", "via", "ICAM-3", "(", "homotypic", "aggregation", ",", "IL-8", "secretion", ")", "were", "clearly", "enhanced", "upon", "RA", "pretreatment", ",", "suggesting", "that", "RA", "may", "contribute", "via", "hitherto", "unrecognized", "pathways", "to", "immune", "function", "and", "host", "defense", "."]} {"tags": [3, 0, 5, 6, 0, 0], "tokens": ["CD28", "and", "T", "cell", "co-stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Over", "the", "last", "decade", "the", "concept", "of", "T", "cell", "co-stimulation", "has", "emerged", "to", "take", "a", "central", "role", "in", "the", "process", "of", "T", "cell", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "exact", "definition", "of", "co-stimulation", "is", "still", "unclear", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "review", ",", "we", "re-examine", "the", "concept", "of", "co-stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0], "tokens": ["We", "suggest", "that", "while", "co-stimulation", "is", "important", ",", "there", "is", "little", "evidence", "to", "link", "co-stimulation", "with", "T", "cell", "anergy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "then", "suggest", "a", "framework", "for", "studying", "co-stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Focusing", "on", "recent", "advances", "in", "our", "understanding", "of", "CD28", ",", "we", "discuss", "four", "areas", "of", "T", "cell", "activation", "where", "co-stimulation", "may", "play", "a", "role", "."]} {"tags": [0, 0, 3, 4, 0, 3, 4, 0, 5, 6, 6, 0, 0, 3, 4, 0], "tokens": ["Regulation", "of", "activator", "protein-1", "and", "NF-kappa", "B", "in", "CD8+", "T", "cells", "exposed", "to", "peripheral", "self-antigens", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["The", "transcriptional", "events", "that", "control", "T", "cell", "tolerance", "to", "peripheral", "self", "Ags", "are", "still", "unknown", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 5, 0], "tokens": ["In", "this", "study", ",", "we", "analyzed", "the", "regulation", "of", "AP-1", "-", "and", "NF-kappa", "B", "-mediated", "transcription", "during", "in", "vivo", "induction", "of", "tolerance", "to", "a", "self", "Ag", "expressed", "exclusively", "on", "hepatocytes", "."]} {"tags": [5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 5, 0, 0], "tokens": ["Naive", "CD8", "(", "+", ")", "Desire", "(", "Des", ")", "(", "+", ")", "T", "cells", "isolated", "from", "the", "Des", "TCR-transgenic", "mice", "that", "are", "specific", "for", "the", "H-2K", "(", "b", ")", "class", "I", "Ag", "were", "transferred", "into", "Alb-K", "(", "b", ")", "-transgenic", "mice", "that", "express", "the", "H-2K", "(", "b", ")", "Ag", "on", "hepatocytes", "only", "."]} {"tags": [0, 0, 0, 0, 0, 0], "tokens": ["Tolerance", "develops", "in", "these", "mice", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "found", "that", "the", "self-reactive", "CD8", "(", "+", ")", "Des", "(", "+", ")", "T", "cells", "were", "transiently", "activated", ",", "then", "became", "unresponsive", "and", "were", "further", "deleted", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["In", "contrast", "to", "CD8", "(", "+", ")", "Des", "(", "+", ")", "T", "cells", "activated", "in", "vivo", "with", "APCs", ",", "which", "express", "high", "AP-1", "and", "high", "NF-kappa", "B", "transcriptional", "activity", ",", "the", "unresponsive", "CD8", "(", "+", ")", "Des", "(", "+", ")", "T", "cells", "expressed", "no", "AP-1", "and", "only", "weak", "NF-kappa", "B", "transcriptional", "activity", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "differences", "in", "NF-kappa", "B", "transcriptional", "activity", "correlated", "with", "the", "generation", "of", "distinct", "NF-kappa", "B", "complexes", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 6, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Indeed", ",", "in", "vivo", "primed", "T", "cells", "predominantly", "express", "p50/p50", "and", "p65/p50", "dimers", ",", "whereas", "these", "p50-containing", "complexes", "are", "barely", "detectable", "in", "tolerant", "T", "cells", "that", "express", "p65-", "and", "c-Rel-containing", "complexes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 5, 6, 0, 0], "tokens": ["These", "observations", "suggest", "that", "fine", "regulation", "of", "NF-kappa", "B", "complex", "formation", "may", "determine", "T", "cell", "fate", "."]} {"tags": [0, 0, 0, 5, 0], "tokens": ["Transcriptional", "regulation", "in", "lymphocytes", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Lymphocytes", "have", "been", "used", "to", "investigate", "many", "cellular", "processes", ",", "including", "lineage", "commitment", ",", "differentiation", ",", "proliferation", "and", "apoptosis", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "transcription", "factors", "that", "mediate", "these", "processes", "are", "often", "expressed", "broadly", "in", "many", "cell", "types", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "emerging", "theme", "is", "one", "of", "cell-type-specific", "regulation", ",", "affecting", "not", "only", "the", "functional", "activation", "of", "transcription", "factors", "but", "also", "their", "access", "to", "appropriate", "regions", "of", "DNA", "."]} {"tags": [0, 0, 3, 4, 0, 3, 4, 0, 0, 0, 5, 6, 0, 0], "tokens": ["Existence", "of", "retinoic", "acid-receptor", "-independent", "retinoid", "X-receptor", "-dependent", "pathway", "in", "myeloid", "cell", "function", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0], "tokens": ["We", "previously", "reported", "that", "ER-27191", "(", "4-", "[", "4", ",", "5", ",", "7", ",", "8", ",", "9", ",", "10-hexahydro-7", ",", "7", ",", "10", ",", "10-tetramethyl-1-", "(", "3-pyridylmethyl", ")", "anthra", "[", "1", ",", "2-b", "]", "pyrrol-3-yl", "]", "benzoic", "acid", ")", "is", "a", "potent", "antagonist", "of", "retinoic", "acid", "receptor", "(", "RAR", ")", ",", "and", "ER-35795", "(", "(", "2E", ",", "4E", ",", "6E", ")", "-7-", "[", "1-", "(", "1-methylethyl", ")", "-8-chloro-1", ",", "2", ",", "3", ",", "4-tetrahydroquinolin-6-yl", "]", "-6-fluoro-3-methyl-2", ",", "4", ",", "6-nonatrienoic", "acid", ")", "is", "a", "novel", "retinoid", "X", "receptor", "(", "RXR", ")", "-specific", "agonist", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["By", "using", "these", "compounds", ",", "we", "investigated", "whether", "distinct", "RAR", "-dependent", "and", "RXR", "-dependent", "pathways", "operate", "to", "mediate", "the", "diverse", "activities", "of", "retinoids", ",", "particularly", ",", "the", "effects", "of", "the", "RXR", "pathway", "on", "cellular", "function", "."]} {"tags": [0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["ER-27191", "completely", "antagonized", "HL60", "cell", "differentiation", "induced", "by", "all-trans-retinoic", "acid", "(", "atRA", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "differentiation", "induced", "by", "the", "ER-35795", "was", "not", "antagonized", "at", "all", "by", "the", "RAR", "antagonist", ",", "but", "was", "inhibited", "by", "an", "RXR", "homodimer", "antagonist", "(", "LGD100754", ",", "(", "2E", ",", "4E", ",", "6Z", ")", "-7-", "(", "3-n-propoxy-5", ",", "6", ",", "7", ",", "8-tetrahydro-5", ",", "5", ",", "8", ",", "8-tetramethylnaphthalen-2-yl", ")", "-3-methylocta-2", ",", "4", ",", "6-trienoic", "acid", ")", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Its", "agonistic", "action", "on", "RXR/RAR", "heterodimer", ",", "on", "the", "other", "hand", ",", "was", "neutralized", "by", "the", "RAR", "antagonist", "."]} {"tags": [0, 7, 8, 0, 0, 0, 0, 9, 10, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["During", "HL60", "cell", "differentiation", ",", "atRA", "induced", "RARbeta", "mRNA", ",", "while", "the", "RXR", "had", "no", "effect", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0], "tokens": ["Interestingly", ",", "a", "functional", "RXR", "-pathway", "was", "also", "seen", "in", "lipopolysaccharide-induced", "inhibition", "of", "mouse", "splenocyte", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["These", "results", "strongly", "suggest", "the", "existence", "of", "a", "pharmacological", "RXR", "-dependent", "pathway", "that", "is", "activated", "by", "a", "ligand", "that", "can", "bind", "to", "RXR", "."]} {"tags": [0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0], "tokens": ["Targeting", "Src", "homology", "2", "domain-containing", "tyrosine", "phosphatase", "(", "SHP-1", ")", "into", "lipid", "rafts", "inhibits", "CD3", "-induced", "T", "cell", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["To", "study", "the", "mechanism", "by", "which", "protein", "tyrosine", "phosphatases", "(", "PTPs", ")", "regulate", "CD3", "-induced", "tyrosine", "phosphorylation", ",", "we", "investigated", "the", "distribution", "of", "PTPs", "in", "subdomains", "of", "plasma", "membrane", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "report", "here", "that", "the", "bulk", "PTP", "activity", "associated", "with", "T", "cell", "membrane", "is", "present", "outside", "the", "lipid", "rafts", ",", "as", "determined", "by", "sucrose", "density", "gradient", "sedimentation", "."]} {"tags": [0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "Jurkat", "T", "cells", ",", "approximately", "5", "--", "10", "%", "of", "Src", "homology", "2", "domain-containing", "tyrosine", "phosphatase", "(", "SHP-1", ")", "is", "constitutively", "associated", "with", "plasma", "membrane", ",", "and", "nearly", "50", "%", "of", "SHP-2", "is", "translocated", "to", "plasma", "membrane", "after", "vanadate", "treatment", "."]} {"tags": [0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 3, 4, 0, 0, 0], "tokens": ["Similar", "to", "transmembrane", "PTP", ",", "CD45", ",", "the", "membrane-associated", "populations", "of", "SHP-1", "and", "SHP-2", "are", "essentially", "excluded", "from", "lipid", "rafts", ",", "where", "other", "signaling", "molecules", "such", "as", "Lck", ",", "linker", "for", "activation", "of", "T", "cells", ",", "and", "CD3", "zeta", "are", "enriched", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["We", "further", "demonstrated", "that", "CD3", "-induced", "tyrosine", "phosphorylation", "of", "these", "substrates", "is", "largely", "restricted", "to", "lipid", "rafts", ",", "unless", "PTPs", "are", "inhibited", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0], "tokens": ["It", "suggests", "that", "a", "restricted", "partition", "of", "PTPs", "among", "membrane", "subdomains", "may", "regulate", "protein", "tyrosine", "phosphorylation", "in", "T", "cell", "membrane", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 4, 4, 0, 0], "tokens": ["To", "test", "this", "hypothesis", ",", "we", "targeted", "SHP-1", "into", "lipid", "rafts", "by", "using", "the", "N-terminal", "region", "of", "Lck", "(", "residues", "1", "--", "14", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["The", "results", "indicate", "that", "the", "expression", "of", "Lck/SHP-1", "chimera", "inside", "lipid", "rafts", "profoundly", "inhibits", "CD3", "-induced", "tyrosine", "phosphorylation", "of", "CD3", "zeta/epsilon", ",", "IL-2", "generation", ",", "and", "nuclear", "mobilization", "of", "NF-AT", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Collectively", ",", "these", "results", "suggest", "that", "the", "exclusion", "of", "PTPs", "from", "lipid", "rafts", "may", "be", "a", "mechanism", "that", "potentiates", "TCR", "/", "CD3", "activation"]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 5, 0, 0, 0, 0, 3, 0], "tokens": ["An", "instructive", "component", "in", "T", "helper", "cell", "type", "2", "(", "Th2", ")", "development", "mediated", "by", "GATA-3", "."]} {"tags": [0, 3, 4, 4, 4, 4, 0, 3, 0, 5, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "interleukin", "(", "IL", ")", "-12", "and", "IL-4", "polarize", "naive", "CD4", "(", "+", ")", "T", "cells", "toward", "T", "helper", "cell", "type", "1", "(", "Th1", ")", "or", "Th2", "phenotypes", ",", "it", "is", "not", "known", "whether", "cytokines", "instruct", "the", "developmental", "fate", "in", "uncommitted", "progenitors", "or", "select", "for", "outgrowth", "of", "cells", "that", "have", "stochastically", "committed", "to", "a", "particular", "fate", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "distinguish", "these", "instructive", "and", "selective", "models", ",", "we", "used", "surface", "affinity", "matrix", "technology", "to", "isolate", "committed", "progenitors", "based", "on", "cytokine", "secretion", "phenotype", "and", "developed", "retroviral-based", "tagging", "approaches", "to", "directly", "monitor", "individual", "progenitor", "fate", "decisions", "at", "the", "clonal", "and", "population", "levels", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "observe", "IL-4", "-dependent", "redirection", "of", "phenotype", "in", "cells", "that", "have", "already", "committed", "to", "a", "non-IL-4-producing", "fate", ",", "inconsistent", "with", "predictions", "of", "the", "selective", "model", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Further", ",", "retroviral", "tagging", "of", "naive", "progenitors", "with", "the", "Th2-specific", "transcription", "factor", "GATA-3", "provided", "direct", "evidence", "for", "instructive", "differentiation", ",", "and", "no", "evidence", "for", "the", "selective", "outgrowth", "of", "cells", "committed", "to", "either", "the", "Th1", "or", "Th2", "fate", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "would", "seem", "to", "exclude", "selection", "as", "an", "exclusive", "mechanism", "in", "Th1", "/", "Th2", "differentiation", ",", "and", "support", "an", "instructive", "model", "of", "cytokine-driven", "transcriptional", "programming", "of", "cell", "fate", "decisions", "."]} {"tags": [0, 0, 5, 6, 6, 6, 0, 0, 3, 4, 4, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["In", "vitro-activated", "human", "lupus", "T", "cells", "express", "normal", "estrogen", "receptor", "proteins", "which", "bind", "to", "the", "estrogen", "response", "element", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["We", "have", "shown", "that", "estrogen", "receptor", "(", "ERalpha", ",", "ERbeta", ")", "transcripts", "are", "expressed", "in", "SLE", "and", "normal", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", ",", "T", "cell", "nuclear", "extracts", "from", "female", "lupus", "patients", "and", "normal", "donors", "were", "tested", "for", "biologically", "active", "ER", "proteins", "capable", "of", "binding", "to", "the", "human", "estrogen", "response", "element", "(", "hERE", ")", "by", "electrophoretic", "mobility", "shift", "assays", "."]} {"tags": [0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["When", "peripheral", "blood", "T", "cells", "were", "stimulated", "with", "17beta-estradiol", "(", "E2", ")", ",", "PMA", "and", "ionomycin", ",", "two", "major", "retarded", "bands", "in", "T", "cell", "nuclear", "extracts", "exhibited", "a", "migration", "pattern", "similar", "to", "slow", "migrating", "protein-ERE", "complexes", "in", "human", "breast", "cancer", "cell", "extracts", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T", "cells", "cultured", "only", "with", "E2", "did", "not", "have", "these", "complexes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["The", "formation", "of", "the", "complexes", "was", "inhibited", "by", "competition", "with", "the", "hERE", "cold", "oligonucleotide", "and", "partially", "with", "anti-ERalpha", "antibodies", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "was", "no", "notable", "difference", "in", "the", "migration", "pattern", "of", "ERE-binding", "proteins", "between", "the", "SLE", "and", "normal", "T", "cell", "extracts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Together", ",", "these", "results", "suggest", "that", "activated", "human", "T", "cells", ",", "whether", "lupus-derived", "or", "normal-derived", ",", "contain", "biologically", "active", "ERalpha", "proteins", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0], "tokens": ["Other", "factors", "may", "be", "responsible", "for", "differential", "sensitivity", "of", "lupus", "T", "cells", "to", "estrogen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 5, 0], "tokens": ["Mechanism", "of", "the", "inhibitory", "effect", "of", "protease", "inhibitor", "on", "tumor", "necrosis", "factor", "alpha", "production", "of", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["If", "the", "inflammatory", "response", "becomes", "excessive", "or", "uncontrolled", "by", "some", "stimuli", ",", "inappropriate", "inflammatory", "responses", "occur", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Monocytes", "are", "extremely", "important", "cells", "for", "regulating", "the", "cytokine", "network", "and", "tumor", "necrosis", "factor", "alpha", "(", "TNFalpha", ")", "and", "interleukin-", "(", "IL", ")", "10", ",", "which", "are", "mainly", "synthesized", "by", "monocytes", ",", "are", "representative", "cytokines", "that", "play", "a", "central", "role", "in", "the", "cytokine", "network", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Protease", "inhibitors", "such", "as", "gabexate", "mesilate", "(", "GM", ")", "and", "ulinastatin", "(", "UTI", ")", "have", "been", "shown", "to", "have", "various", "beneficial", "effects", "by", "inhibiting", "the", "activation", "of", "leukocytes", ",", "but", "the", "mechanism", "for", "this", "has", "yet", "to", "be", "fully", "elucidated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["In", "this", "study", "we", "investigated", "the", "mechanism", "of", "the", "inhibitory", "effect", "of", "protease", "inhibitors", "on", "the", "proinflammatory", "cytokine", "production", "of", "lipopolysaccharide-", "(", "LPS", ")", "stimulated", "monocytes", "."]} {"tags": [0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["LPS-stimulated", "monocytes", "were", "treated", "with", "GM", "or", "UTI", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "value", "of", "TNFalpha", "and", "IL-10", "in", "the", "culture", "medium", "of", "monocytes", "was", "measured", "and", "each", "mRNA", "expression", "was", "assayed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0], "tokens": ["The", "inhibitory", "effect", "of", "protease", "inhibitors", "on", "the", "activity", "of", "intracellular", "signal", "transduction", "pathways", "such", "as", "protein", "kinase", "C", "(", "PKC", ")", "and", "nuclear", "factor", "kappa", "B", "(", "NFkappaB", ")", "were", "also", "evaluated", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 0], "tokens": ["GM", "decreased", "the", "TNFalpha", "production", "of", "LPS-stimulated", "monocytes", "as", "shown", "by", "the", "inhibition", "of", "mRNA", "expression", "and", "increased", "the", "IL-10", "production", "of", "LPS-stimulated", "monocytes", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 5, 0], "tokens": ["GM", "also", "suppressed", "the", "NFkappaB", "activity", "of", "LPS-stimulated", "monocytes", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0], "tokens": ["UTI", "decreased", "the", "TNFalpha", "production", "of", "LPS-stimulated", "monocytes", ",", "but", "did", "not", "inhibit", "the", "TNFalpha", "mRNA", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["The", "present", "study", "shows", "that", "the", "inhibitory", "effect", "of", "GM", "on", "the", "TNFalpha", "production", "of", "activated", "human", "monocytes", "is", "mediated", "by", "the", "suppression", "of", "NFkappaB", "activation", ",", "while", "the", "mechanism", "of", "UTI", "inhibiting", "TNFalpha", "production", "of", "human", "monocytes", "may", "be", "due", "to", "the", "inhibition", "of", "either", "the", "translation", "or", "secretion", "of", "TNFalpha", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Homocysteine", "stimulates", "the", "expression", "of", "monocyte", "chemoattractant", "protein-1", "in", "endothelial", "cells", "leading", "to", "enhanced", "monocyte", "chemotaxis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Hyperhomocysteinemia", "has", "been", "identified", "as", "an", "independent", "risk", "factor", "for", "atherosclerosis", "."]} {"tags": [0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "infiltration", "of", "monocytes", "into", "the", "arterial", "wall", "is", "one", "of", "the", "key", "events", "during", "atherogenesis", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Monocyte", "chemoattractant", "protein-1", "(", "MCP-1", ")", "is", "a", "potent", "chemokine", "that", "stimulates", "the", "migration", "of", "monocytes", "into", "the", "intima", "of", "the", "arterial", "wall", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "mechanism", "by", "which", "increased", "monocyte", "infiltration", "occurs", "in", "atherosclerotic", "lesions", "in", "patients", "with", "hyperhomocysteinemia", "has", "not", "been", "delineated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 0], "tokens": ["The", "objective", "of", "the", "present", "study", "was", "to", "investigate", "the", "effect", "of", "homocysteine", "on", "MCP-1", "production", "in", "endothelial", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0], "tokens": ["Cells", "were", "incubated", "with", "homocysteine", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "secretion", "of", "MCP-1", "protein", "was", "significantly", "increased", "(", "195", "%", "as", "compared", "to", "the", "control", ")", "in", "cells", "treated", "with", "pathological", "concentrations", "of", "homocysteine", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Such", "effect", "was", "accompanied", "by", "an", "increased", "expression", "of", "MCP-1", "mRNA", "(", "176", "%", "as", "compared", "to", "the", "control", ")", "in", "endothelial", "cells", "which", "resulted", "in", "enhanced", "monocyte", "chemotaxis", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "p38", "MAP", "kinase", "as", "well", "as", "other", "members", "of", "the", "p38", "MAP", "kinase", "pathway", ",", "including", "MKK3", ",", "MKK6", ",", "ATF-2", "and", "Elk-1", ",", "were", "activated", "in", "homocysteine-treated", "cells", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 0], "tokens": ["Homocysteine-induced", "MCP-1", "expression", "and", "subsequent", "monocyte", "chemotaxis", "were", "blocked", "by", "a", "p38", "MAP", "kinase", "inhibitor", "(", "SB203580", ")", "suggesting", "that", "the", "p38", "MAP", "kinase", "pathway", "might", "be", "involved", "in", "homocysteine-induced", "MCP-1", "expression", "in", "endothelial", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["In", "contrast", ",", "staurosporine", ",", "a", "protein", "kinase", "C", "inhibitor", ",", "had", "no", "effect", "on", "homocysteine-induced", "MCP-1", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["In", "conclusion", ",", "our", "results", "indicate", "that", "homocysteine", "stimulates", "MCP-1", "expression", "in", "endothelial", "cells", "leading", "to", "enhanced", "monocyte", "chemotaxis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Inducible", "resistance", "to", "Fas-mediated", "apoptosis", "in", "B", "cells", "."]} {"tags": [0, 0, 0, 5, 6, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Apoptosis", "produced", "in", "B", "cells", "through", "Fas", "(", "APO-1", ",", "CD95", ")", "triggering", "is", "regulated", "by", "signals", "derived", "from", "other", "surface", "receptors", ":", "CD40", "engagement", "produces", "upregulation", "of", "Fas", "expression", "and", "marked", "susceptibility", "to", "Fas", "-induced", "cell", "death", ",", "whereas", "antigen", "receptor", "engagement", ",", "or", "IL-4R", "engagement", ",", "inhibits", "Fas", "killing", "and", "in", "so", "doing", "induces", "a", "state", "of", "Fas", "-resistance", ",", "even", "in", "otherwise", "sensitive", ",", "CD40", "-stimulated", "targets", "."]} {"tags": [3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0], "tokens": ["Surface", "immunoglobulin", "and", "IL-4R", "utilize", "at", "least", "partially", "distinct", "pathways", "to", "produce", "Fas", "-resistance", "that", "differentially", "depend", "on", "PKC", "and", "STAT6", ",", "respectively", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Further", ",", "surface", "immunoglobulin", "signaling", "for", "inducible", "Fas", "-resistance", "bypasses", "Btk", ",", "requires", "NF-kappaB", ",", "and", "entails", "new", "macromolecular", "synthesis", "."]} {"tags": [0, 0, 0, 5, 6, 3, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 1, 2, 0, 0, 3, 0, 3, 4, 4, 4, 0, 0], "tokens": ["Terminal", "effectors", "of", "B", "cell", "Fas", "-resistance", "include", "the", "known", "anti-apoptotic", "gene", "products", ",", "Bcl-xL", "and", "FLIP", ",", "and", "a", "novel", "anti-apoptotic", "gene", "that", "encodes", "FAIM", "(", "Fas", "Apoptosis", "Inhibitory", "Molecule", ")", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["faim", "was", "identified", "by", "differential", "display", "and", "exists", "in", "two", "alternatively", "spliced", "forms", ";", "faim-S", "is", "broadly", "expressed", ",", "but", "faim-L", "expression", "is", "tissue-specific", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "FAIM", "sequence", "is", "highly", "evolu-", "tionarily", "conserved", ",", "suggesting", "an", "important", "role", "for", "this", "molecule", "throughout", "phylogeny", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 5, 6, 6, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Inducible", "resistance", "to", "Fas", "killing", "is", "hypothesized", "to", "protect", "foreign", "antigen-specific", "B", "cells", "during", "potentially", "hazardous", "interactions", "with", "FasL-bearing", "T", "cells", ",", "whereas", "autoreactive", "B", "cells", "fail", "to", "become", "Fas", "-resistant", "and", "are", "deleted", "via", "Fas", "-dependent", "cytotoxicity", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 6, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Inadvertent", "or", "aberrant", "acquisition", "of", "Fas", "-resistance", "may", "permit", "autoreactive", "B", "cells", "to", "escape", "Fas", "deletion", ",", "and", "malignant", "lymphocytes", "to", "impede", "anti-tumor", "immunity", "."]} {"tags": [3, 4, 4, 4, 4, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Stromal", "cell-derived", "factor", "1", "alpha", "-induced", "chemotaxis", "in", "T", "cells", "is", "mediated", "by", "nitric", "oxide", "signaling", "pathways", "."]} {"tags": [3, 4, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 5, 0], "tokens": ["Stromal", "cell-derived", "factor", "1", "alpha", "(", "SDF1", "alpha", ")", "and", "its", "cognate", "chemokine", "receptor", "CXCR4", "act", "as", "potent", "chemoattractants", "and", "regulate", "trafficking", "and", "homing", "of", "hematopoietic", "progenitor", "cells", "and", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "molecular", "mechanisms", "regulating", "SDF1", "alpha", "-driven", "cell", "migration", "are", "not", "well", "defined", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", ",", "we", "have", "explored", "the", "roles", "of", "the", "second", "messenger", "NO", "and", "the", "transcription", "factor", "NF-kappa", "B", "in", "SDF1", "alpha", "-induced", "T", "cell", "migration", "."]} {"tags": [3, 4, 0, 0, 7, 8, 8, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["SDF1", "alpha", "treatment", "of", "Jurkat", "T", "cells", "increased", "the", "activity", "of", "NO", "synthase", ",", "which", "catalyzes", "the", "generation", "of", "NO", "."]} {"tags": [0, 0, 0, 0, 0, 7, 8, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "observed", "that", "pretreatment", "of", "Jurkat", "cells", "or", "activated", "PBLs", "with", "several", "NO", "donors", "significantly", "enhanced", "the", "SDF1", "alpha", "-induced", "migration", ",", "whereas", "various", "inhibitors", "of", "NO", "synthase", "markedly", "abrogated", "the", "chemotactic", "response", "in", "a", "concentration-dependent", "manner", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "we", "observed", "that", "inhibitors", "of", "the", "transcription", "factor", "NF-kappa", "B", ",", "which", "is", "linked", "to", "NO", "signaling", "pathways", ",", "also", "significantly", "blocked", "the", "SDF1", "alpha", "-induced", "chemotactic", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "these", "compounds", "did", "not", "have", "a", "significant", "effect", "on", "SDF1", "alpha", "-induced", "mitogen-activated", "protein", "kinase", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["In", "addition", ",", "the", "MAP/Erk", "kinase", "kinase", "inhibitor", "PD98059", "did", "not", "abrogate", "SDF1", "alpha", "-induced", "chemotaxis", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0], "tokens": ["AKT", ",", "which", "has", "been", "shown", "to", "mediate", "NO", "production", ",", "was", "also", "phosphorylated", "upon", "SDF1", "alpha", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0], "tokens": ["These", "studies", "suggest", "that", "NO-related", "signaling", "pathways", "may", "mediate", "SDF1", "alpha", "-induced", "chemotaxis", ",", "but", "not", "mitogen-activated", "protein", "kinase", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["The", "lack", "of", "NF-kappa", "B", "transactivation", "and", "PKC", "epsilon", "expression", "in", "CD4", "(", "+", ")", "CD8", "(", "+", ")", "thymocytes", "correlates", "with", "negative", "selection", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Deletion", "of", "autoreactive", "thymocytes", "at", "the", "DP", "stage", "is", "the", "basis", "for", "tolerance", "to", "thymus-expressed", "self", "antigens", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", "we", "investigated", "whether", "distinct", "signalling", "pathways", "are", "induced", "in", "DP", "thymocytes", "as", "compared", "to", "mature", "T", "cells", "upon", "stimulation", "with", "antigen", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 0, 3, 4, 4, 4, 0, 0, 1, 2, 2, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["Using", "triple", "transgenic", "mice", "expressing", "a", "TCR", "transgene", ",", "dominant", "negative", "ras/Mek", "proteins", "and", "a", "reporter", "gene", "construct", "with", "AP-1", "or", "NF-kappa", "B", "binding", "sites", ",", "we", "showed", "a", "complete", "lack", "of", "transcriptional", "activity", "of", "NF-kappa", "B", "but", "not", "AP-1", "in", "DP", "thymocytes", ",", "whereas", "both", "were", "transcriptionally", "active", "in", "mature", "T", "cells", "after", "antigenic", "stimulation", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Lack", "of", "NF-kappa", "B", "induction", "correlated", "with", "increased", "death", "in", "response", "to", "antigen", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["AP-1", "induction", "was", "dependent", "on", "the", "integrity", "of", "the", "ras/Mek", "pathway", "indicating", "that", "this", "pathway", "was", "activated", "in", "the", "DP", "thymocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 6, 0], "tokens": ["In", "contrast", ",", "we", "found", "a", "complete", "lack", "of", "constitutive", "expression", "of", "the", "epsilon", "isoform", "of", "Protein", "Kinase", "C", "(", "PKC", ")", "in", "DP", "thymocytes", ",", "although", "it", "was", "present", "in", "mature", "thymocytes", "and", "peripheral", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Taken", "together", "the", "results", "suggest", "that", "the", "lack", "of", "PKC", "epsilon", "in", "DP", "thymocytes", "could", "lead", "to", "the", "absence", "of", "NF-kappa", "B", "activity", "after", "antigenic", "stimulation", "contributing", "to", "negative", "selection", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cell", "Death", "and", "Differentiation", "(", "2000", ")", "7", ",", "1253", "-", "1262", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 5, 6, 0], "tokens": ["CD2", "stimulation", "leads", "to", "the", "delayed", "and", "prolonged", "activation", "of", "STAT1", "in", "T", "cells", "but", "not", "NK", "cells", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["OBJECTIVE", ":", "T", "lymphocytes", "can", "be", "activated", "by", "soluble", "factors", "such", "as", "cytokines", "or", "through", "direct", "cell-cell", "interactions", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "cytokine", "receptors", "are", "known", "to", "signal", "through", "STAT", "family", "transcription", "factors", ",", "the", "mechanisms", "by", "which", "other", "cell-surface", "molecules", ",", "such", "as", "CD2", ",", "transduce", "signals", "is", "unclear", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "goal", "of", "this", "study", "was", "to", "determine", "whether", "stimulation", "of", "T", "cells", "through", "CD2", "recapitulates", "aspects", "of", "cytokine", "-induced", "T-cell", "activation", "by", "use", "of", "STAT", "transcription", "factors", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0], "tokens": ["MATERIALS", "AND", "METHODS", ":", "T", "cells", "were", "treated", "with", "anti-CD2", "antibodies", "or", "cells", "bearing", "the", "natural", "CD2", "ligand", "CD58", ",", "after", "which", "signaling", "through", "STAT", "transcription", "factors", "was", "assessed", "."]} {"tags": [0, 0, 0, 0, 3, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["RESULTS", ":", "Stimulation", "of", "CD2", "on", "primary", "T", "lymphocytes", "leads", "to", "the", "tyrosine", "phosphorylation", ",", "nuclear", "translocation", ",", "and", "DNA", "binding", "of", "STAT1", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["In", "contrast", "to", "stimulation", "by", "cytokines", ",", "the", "activation", "of", "STAT1", "in", "response", "to", "CD2", "ligation", "is", "delayed", "and", "does", "not", "involve", "Jak", "kinases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "while", "STAT", "phosphorylation", "induced", "by", "cytokines", "is", "generally", "transient", ",", "STAT1", "phosphorylation", "following", "CD2", "stimulation", "persists", "for", "a", "period", "of", "days", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transcription", "of", "key", "target", "genes", "such", "as", "IRF1", "and", "c-fos", "proceeds", "with", "delayed", "kinetics", "following", "CD2", "stimulation", ",", "suggesting", "that", "this", "unique", "pattern", "of", "STAT", "activation", "may", "lead", "to", "a", "distinct", "cellular", "response", "following", "CD2", "ligation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "pathway", "appears", "to", "be", "restricted", "to", "T", "cells", ",", "as", "stimulation", "of", "CD2", "on", "NK", "cells", "does", "not", "lead", "to", "STAT1", "activation", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 3, 4, 0, 0, 3, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSION", ":", "Stimulation", "of", "T", "cells", "through", "cell-surface", "molecules", "such", "as", "CD2", "involves", "activation", "of", "STAT", "transcription", "factors", ",", "thus", "recapitulating", "elements", "of", "cytokine", "signaling", "."]} {"tags": [3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NFATc1", "and", "NFATc2", "together", "control", "both", "T", "and", "B", "cell", "activation", "and", "differentiation", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NFAT", "transcription", "factors", "play", "critical", "roles", "in", "gene", "transcription", "during", "immune", "responses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "investigate", "further", "the", "two", "most", "prominent", "NFAT", "family", "members", ",", "NFATc1", "and", "NFATc2", ",", "we", "generated", "mice", "bearing", "lymphoid", "systems", "devoid", "of", "both", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Doubly", "deficient", "T", "cells", "displayed", "cell", "surface", "markers", "of", "activation", "yet", "were", "significantly", "deficient", "in", "the", "development", "of", "multiple", "effector", "functions", ",", "including", "Th", "cytokine", "production", ",", "surface", "effector", "molecule", "expression", ",", "and", "cytolytic", "activity", "."]} {"tags": [0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nevertheless", ",", "doubly", "deficient", "B", "cells", "were", "hyperactivated", ",", "as", "evidenced", "by", "extremely", "elevated", "serum", "IgG1", "and", "IgE", ",", "as", "well", "as", "plasma", "cell", "expansion", "and", "infiltration", "of", "end", "organs", "."]} {"tags": [0, 0, 0, 5, 6, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "in", "T", "cells", ",", "NFATc1", "and", "NFATc2", "are", "dispensable", "for", "inflammatory", "reactivity", "but", "are", "required", "for", "effector", "differentiation", ",", "while", "in", "B", "cells", ",", "NFATs", "regulate", "both", "normal", "homeostasis", "and", "differentiation", "."]} {"tags": [0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Epstein-barr", "virus", "immediate-early", "protein", "BZLF1", "is", "SUMO-1", "modified", "and", "disrupts", "promyelocytic", "leukemia", "bodies", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "the", "immediate-early", "proteins", "of", "both", "herpes", "simplex", "virus", "(", "HSV", ")", "and", "cytomegalovirus", "(", "CMV", ")", "are", "known", "to", "modify", "promyelocytic", "leukemia", "(", "PML", ")", "(", "ND10", ")", "bodies", "in", "the", "nucleus", "of", "the", "host", "cell", ",", "it", "has", "been", "unclear", "whether", "lytic", "infection", "with", "gamma", "herpesviruses", "induces", "a", "similar", "effect", "."]} {"tags": [0, 3, 4, 0, 0, 0, 3, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "PML", "protein", "is", "induced", "by", "interferon", ",", "involved", "in", "major", "histocompatibility", "complex", "class", "I", "presentation", ",", "and", "necessary", "for", "certain", "types", "of", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", ",", "it", "is", "likely", "that", "PML", "bodies", "function", "in", "an", "antiviral", "capacity", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["SUMO-1", "modification", "of", "PML", "is", "known", "to", "be", "required", "for", "the", "formation", "of", "PML", "bodies", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 1, 2, 2, 2, 0, 1, 2, 2, 2, 0, 7, 8, 8, 0, 0, 3, 0, 0], "tokens": ["To", "examine", "whether", "Epstein-Barr", "virus", "(", "EBV", ")", "lytic", "replication", "interferes", "with", "PML", "bodies", ",", "we", "expressed", "the", "EBV", "immediate-early", "genes", "BZLF1", "(", "Z", ")", "and", "BRLF1", "(", "R", ")", "in", "EBV-positive", "cell", "lines", "and", "examined", "PML", "localization", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 0], "tokens": ["Both", "Z", "and", "R", "expression", "resulted", "in", "PML", "dispersion", "in", "EBV-positive", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Z", "but", "not", "R", "expression", "is", "sufficient", "to", "disrupt", "PML", "bodies", "in", "EBV-negative", "cell", "lines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0], "tokens": ["We", "show", "that", "dispersion", "of", "PML", "bodies", "by", "Z", "requires", "a", "portion", "of", "the", "transcriptional", "activation", "domain", "of", "Z", "but", "not", "the", "DNA-binding", "function", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 1, 0, 0, 0, 0, 3, 4, 0], "tokens": ["As", "was", "previously", "reported", "for", "the", "HSV-1", "ICP0", "and", "CMV", "IE1", "proteins", ",", "Z", "reduces", "the", "amount", "of", "SUMO-1-modified", "PML", "."]} {"tags": [0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 3, 0, 0, 0, 0, 3, 0], "tokens": ["We", "also", "found", "that", "Z", "itself", "is", "SUMO-1", "modified", "(", "through", "amino", "acid", "12", ")", "and", "that", "Z", "competes", "with", "PML", "for", "limiting", "amounts", "of", "SUMO-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "suggest", "that", "disruption", "of", "PML", "bodies", "is", "important", "for", "efficient", "lytic", "replication", "of", "EBV", "."]} {"tags": [0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "Z", "may", "potentially", "alter", "the", "function", "of", "a", "variety", "of", "cellular", "proteins", "by", "inhibiting", "SUMO-1", "modification"]} {"tags": [0, 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Suppression", "of", "nuclear", "factor-kappaB", "and", "stimulation", "of", "inhibitor", "kappaB", "by", "troglitazone", ":", "evidence", "for", "an", "anti-inflammatory", "effect", "and", "a", "potential", "antiatherosclerotic", "effect", "in", "the", "obese", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0], "tokens": ["To", "elucidate", "whether", "troglitazone", "exerts", "an", "antiinflammatory", "effect", "in", "humans", ",", "in", "vivo", ",", "we", "investigated", "the", "suppression", "of", "nuclear", "factor", "kappaB", "(", "NFkappaB", ")", "in", "mononuclear", "cells", "(", "MNC", ")", "by", "this", "drug", "."]} {"tags": [0, 0, 3, 4, 0, 0, 3, 4, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 5, 0], "tokens": ["We", "measured", "intranuclear", "NFkappaB", ",", "total", "cellular", "NFkappaB", ",", "inhibitor", "kappaB", "(", "IkappaB", ")", "alpha", ",", "reactive", "oxygen", "species", "(", "ROS", ")", "generation", ",", "and", "p47", "(", "phox", ")", "subunit", "(", "a", "key", "component", "protein", "of", "nicotinamide", "adenine", "dinucleotide", "phosphate", "oxidase", ")", "in", "MNC", "."]} {"tags": [3, 4, 4, 4, 4, 4, 4, 4, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 4, 0, 0, 3, 4, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Plasma", "tumor", "necrosis", "factor", "(", "TNF", ")", "-alpha", ",", "soluble", "intercellular", "adhesion", "molecule-1", "(", "sICAM-1", ")", ",", "monocyte", "chemoattractant", "protein-1", "(", "MCP-1", ")", ",", "plasminogen", "activator", "inhibitor", "type", "1", "(", "PAI-1", ")", ",", "C-reactive", "protein", "(", "CRP", ")", ",", "and", "interleukin", "(", "IL", ")", "-10", "(", "antiinflammatory", "cytokine", ")", "concentrations", "were", "also", "measured", "as", "mediators", "of", "inflammatory", "activity", "that", "are", "regulated", "by", "the", "proinflammatory", "transcription", "factor", "NFkappaB", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Seven", "nondiabetic", "obese", "patients", "were", "given", "400", "mg", "troglitazone", "daily", "for", "4", "weeks", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Blood", "samples", "were", "collected", "before", "and", "at", "weekly", "intervals", "thereafter", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 3, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["MNC", "were", "separated", ";", "and", "the", "levels", "of", "intranuclear", "NFkappaB", ",", "total", "cellular", "NFkappaB", ",", "IkappaBalpha", ",", "and", "p47", "(", "phox", ")", "subunit", "and", "ROS", "generation", "were", "determined", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0], "tokens": ["Plasma", "was", "used", "to", "measure", "insulin", "glucose", ",", "TNFalpha", ",", "sICAM", ",", "MCP-1", ",", "PAI-1", ",", "CRP", ",", "and", "IL-10", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Plasma", "insulin", "concentrations", "fell", "significantly", "at", "week", "1", ",", "from", "31.2", "+/-", "29.1", "to", "14.2", "+/-", "11.4", "mU/L", "(", "P", "<", "0.01", ")", "and", "remained", "low", "throughout", "4", "weeks", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Plasma", "glucose", "concentrations", "did", "not", "alter", "significantly", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "was", "a", "fall", "in", "intranuclear", "NFkappaB", ",", "total", "cellular", "NFkappaB", ",", "and", "p47", "(", "phox", ")", "subunit", ",", "with", "an", "increase", "in", "cellular", "IkappaBalpha", "at", "week", "2", ",", "which", "persisted", "until", "week", "4", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "was", "a", "parallel", "fall", "in", "ROS", "generation", "by", "MNC", "at", "week", "1", ";", "this", "progressed", "and", "persisted", "until", "week", "4", "(", "P", "<", "0.001", ")", "."]} {"tags": [0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Plasma", "TNF-alpha", ",", "sICAM-1", ",", "MCP-1", ",", "and", "PAI-1", "concentrations", "fell", "significantly", "at", "week", "4", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["Plasma", "IL-10", "concentration", "increased", "significantly", ",", "whereas", "plasma", "CRP", "concentrations", "decreased", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "conclude", "that", "troglitazone", "has", "an", "antiinflammatory", "action", "that", "may", "contribute", "to", "its", "putative", "antiatherosclerotic", "effects", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0], "tokens": ["Tyrosine", "phosphorylation-dependent", "activation", "of", "NF-kappa", "B", "."]} {"tags": [0, 0, 3, 4, 4, 4, 4, 4, 4, 0], "tokens": ["Requirement", "for", "p56", "LCK", "and", "ZAP-70", "protein", "tyrosine", "kinases", "."]} {"tags": [0, 0, 0, 3, 4, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Phosphorylation", "of", "the", "N-terminal", "domain", "of", "I", "kappa", "B", "inhibitory", "subunits", "induces", "activation", "of", "the", "transcription", "factor", "NF-kappa", "B", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "serine", "phosphorylation", "has", "been", "shown", "to", "induce", "ubiquitination", "and", "subsequent", "proteasome-mediated", "degradation", "of", "I", "kappa", "B-alpha", ",", "little", "is", "known", "about", "the", "mechanisms", "that", "lead", "to", "release", "of", "active", "NF-kappa", "B", "in", "T", "cells", "as", "a", "consequence", "of", "tyrosine", "phosphorylation", "of", "I", "kappa", "B-alpha", "[", "Imbert", ",", "V.", ",", "Rupec", ",", "R.A.", ",", "Livolsi", ",", "A.", ",", "Pahl", ",", "H.L.", ",", "Traenckner", ",", "B.M.", ",", "Mueller-Dieckmann", ",", "C.", ",", "Farahifar", ",", "D.", ",", "Rossi", ",", "B.", ",", "Auberger", ",", "P.", ",", "Baeuerle", ",", "P.", "&", "Peyron", ",", "J.F.", "(", "1996", ")", "Cell", "86", ",", "787", "--", "798", "]", "."]} {"tags": [0, 0, 0, 0, 3, 4, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 3, 0], "tokens": ["The", "involvement", "of", "the", "tyrosine", "kinases", "p56", "(", "lck", ")", "and", "ZAP-70", "in", "this", "reaction", "is", "demonstrated", "here", "using", "specific", "pharmacological", "inhibitors", "and", "Jurkat", "mutants", "unable", "to", "express", "these", "kinases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 0, 3, 4, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0], "tokens": ["Although", "the", "inhibitors", "prevented", "both", "pervanadate-induced", "phosphorylation", "of", "I", "kappa", "B-alpha", "on", "Tyr42", "and", "NF-kappa", "B", "activation", ",", "we", "observed", "that", ",", "in", "p56", "(", "lck", ")", "-deficient", "Jurkat", "mutants", ",", "NF-kappa", "B", "could", "still", "associate", "with", "I", "kappa", "B-alpha", "despite", "phosphorylation", "on", "Tyr42", "."]} {"tags": [0, 0, 0, 3, 4, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "the", "SH2", "domain", "of", "p56", "(", "lck", ")", "appeared", "to", "be", "required", "for", "pervanadate-induced", "NF-kappa", "B", "activation", "but", "not", "for", "Tyr42", "phosphorylation", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["These", "results", "show", "that", "p56", "(", "lck", ")", "and", "ZAP-70", "are", "key", "components", "of", "the", "signaling", "pathway", "that", "leads", "to", "phosphotyrosine-dependent", "NF-kappa", "B", "activation", "in", "T", "cells", "and", "confirm", "that", "tyrosine", "kinases", "must", "control", "at", "least", "two", "different", "steps", "to", "induce", "activation", "of", "NF-kappa", "B", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Finally", ",", "we", "show", "that", "H", "(", "2", ")", "O", "(", "2", ")", ",", "which", "stimulates", "p56", "(", "lck", ")", "and", "ZAP-70", "in", "T", "cells", ",", "is", "an", "activator", "of", "NF-kappa", "B", "through", "tyrosine", "phosphorylation", "of", "I", "kappa", "B-alpha", "."]} {"tags": [0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Specific", "missense", "mutations", "in", "NEMO", "result", "in", "hyper-IgM", "syndrome", "with", "hypohydrotic", "ectodermal", "dysplasia", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "gene", "that", "encodes", "nuclear", "factor", "kappaB", "(", "NF-kappaB", ")", "essential", "modulator", "(", "or", "NEMO", ",", "also", "known", "as", "IKKgamma", ")", "is", "required", "for", "activation", "of", "the", "transcription", "factor", "NF-kappaB", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "describe", "mutations", "in", "the", "putative", "zinc-finger", "domain", "of", "NEMO", "that", "result", "in", "an", "X-linked", "primary", "immunodeficiency", "characterized", "by", "hyper-IgM", "syndrome", "and", "hypohydrotic", "ectodermal", "dysplasia", "(", "XHM-ED", ")", "."]} {"tags": [0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0, 0, 0, 0, 0, 3, 4, 3, 4, 0, 3, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 3, 0], "tokens": ["These", "mutations", "prevent", "CD40", "ligand", "(", "CD40L", ")", "-mediated", "degradation", "of", "inhibitor", "of", "NF-kappaB", "alpha", "(", "IkappaB-alpha", ")", "and", "account", "for", "the", "following", "observations", ":", "B", "cells", "from", "XHM-ED", "patients", "are", "unable", "to", "undergo", "immunoglobulin", "class-switch", "recombination", "and", "antigen-presenting", "cells", "(", "APCs", ")", "are", "unable", "to", "synthesize", "the", "NF-kappaB-regulated", "cytokines", "interleukin", "12", "(", "IL-12", ")", "or", "tumor", "necrosis", "factor", "alpha", "(", "TNF-alpha", ")", "when", "stimulated", "with", "CD40L", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0], "tokens": ["Nevertheless", ",", "innate", "immunity", "is", "preserved", "in", "XHM-ED", "patients", "because", "APCs", "retain", "the", "capacity", "to", "respond", "to", "stimulation", "by", "lipopolysaccharide", "or", "Staphylococcus", "aureus", "Cowan", "'s", "antigen", "(", "SAC", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["Overall", ",", "the", "phenotype", "observed", "in", "XHM-ED", "patients", "shows", "that", "the", "putative", "zinc-finger", "domain", "of", "NEMO", "has", "a", "regulatory", "function", "and", "demonstrates", "the", "definite", "requirement", "of", "CD40", "-mediated", "NF-kappaB", "activation", "for", "B", "cell", "immunoglobulin", "class-switching", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Granulocytic", "differentiation", "of", "human", "NB4", "promyelocytic", "leukemia", "cells", "induced", "by", "all-trans", "retinoic", "acid", "metabolites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "metabolism", "of", "all-trans", "retinoic", "acid", "(", "ATRA", ")", "has", "been", "reported", "to", "be", "partly", "responsible", "for", "the", "in", "vivo", "resistance", "to", "ATRA", "seen", "in", "the", "treatment", "of", "human", "acute", "promyelocytic", "leukemia", "(", "APL", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0], "tokens": ["However", ",", "ATRA", "metabolism", "appears", "to", "be", "involved", "in", "the", "growth", "inhibition", "of", "several", "cancer", "cell", "lines", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["The", "purpose", "of", "this", "study", "was", "to", "evaluate", "the", "in", "vitro", "activity", "of", "the", "principal", "metabolites", "of", "ATRA", "[", "4-hydroxy-retinoic", "acid", "(", "4-OH-RA", ")", ",", "18-hydroxy-retinoic", "acid", "(", "18-OH-RA", ")", ",", "4-oxo-retinoic", "acid", "(", "4-oxo-RA", ")", ",", "and", "5", ",", "6-epoxy-retinoic", "acid", "(", "5", ",", "6-epoxy-RA", ")", "]", "in", "NB4", ",", "a", "human", "promyelocytic", "leukemia", "cell", "line", "that", "exhibits", "the", "APL", "diagnostic", "t", "(", "15", ";", "17", ")", "chromosomal", "translocation", "and", "expresses", "the", "PML-RAR", "alpha", "fusion", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0], "tokens": ["We", "established", "that", "the", "four", "ATRA", "metabolites", "were", "indeed", "formed", "by", "the", "NB4", "cells", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NB4", "cell", "growth", "was", "inhibited", "(", "69-78", "%", "at", "120", "h", ")", "and", "cell", "cycle", "progression", "in", "the", "G1", "phase", "(", "82-85", "%", "at", "120", "h", ")", "was", "blocked", "by", "ATRA", "and", "all", "of", "the", "metabolites", "at", "1", "microM", "concentration", "."]} {"tags": [0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0], "tokens": ["ATRA", "and", "its", "metabolites", "could", "induce", "NB4", "cells", "differentiation", "with", "similar", "activity", ",", "as", "evaluated", "by", "cell", "morphology", ",", "by", "the", "nitroblue", "tetrazolium", "reduction", "test", "(", "82-88", "%", "at", "120", "h", ")", "or", "by", "the", "expression", "of", "the", "maturation", "specific", "cell", "surface", "marker", "CD11c", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "nuclear", "body", "reorganization", "to", "macropunctated", "structures", ",", "as", "well", "as", "the", "degradation", "of", "PML-RAR", "alpha", ",", "was", "found", "to", "be", "similar", "for", "ATRA", "and", "all", "of", "its", "metabolites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Comparison", "of", "the", "relative", "potency", "of", "the", "retinoids", "using", "the", "nitroblue", "tetrazolium", "reduction", "test", "showed", "effective", "concentrations", "required", "to", "differentiate", "50", "%", "of", "cells", "in", "72", "h", "as", "follows", ":", "ATRA", ",", "15.8", "+/-", "1.7", "nM", ";", "4-oxo-RA", ",", "38.3", "+/-", "1.3", "nM", ";", "18-OH-RA", ",", "55.5", "+/-", "1.8", "nM", ";", "4-OH-RA", ",", "79.8", "+/-", "1.8", "nM", ";", "and", "5", ",", "6-epoxy-RA", ",", "99.5", "+/-", "1.5", "nM", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["The", "ATRA", "metabolites", "were", "found", "to", "exert", "their", "differentiation", "effects", "via", "the", "RAR", "alpha", "nuclear", "receptors", ",", "because", "the", "RAR", "alpha-specific", "antagonist", "BMS614", "blocked", "metabolite-induced", "CD11c", "expression", "in", "NB4", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "demonstrate", "that", "the", "principal", "ATRA", "Phase", "1", "metabolites", "can", "elicit", "leukemia", "cell", "growth", "inhibition", "and", "differentiation", "in", "vitro", "through", "the", "RAR", "alpha", "signaling", "pathway", ",", "and", "they", "suggest", "that", "these", "metabolites", "may", "play", "a", "role", "in", "ATRA", "antileukemic", "activity", "in", "vivo", "."]} {"tags": [0, 0, 3, 4, 4, 4, 0, 5, 6, 0, 0, 0, 0, 5, 0, 5, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Expression", "of", "oestrogen", "and", "progesterone", "receptors", "by", "mast", "cells", "alone", ",", "but", "not", "lymphocytes", ",", "macrophages", "or", "other", "immune", "cells", "in", "human", "upper", "airways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["BACKGROUND", ":", "Nasal", "polyposis", "often", "coexists", "with", "asthma", "in", "airway", "inflammatory", "conditions", "characterised", "by", "the", "infiltration", "of", "a", "range", "of", "immune", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "potentially", "important", "role", "for", "ovarian", "hormones", "has", "been", "implicated", "in", "airway", "inflammation", "but", "the", "cellular", "target", "for", "such", "action", "is", "not", "known", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Expression", "of", "oestrogen", "receptors", "(", "ER", ")", "and", "progesterone", "receptors", "(", "PR", ")", "was", "examined", "using", "immunohistochemistry", "in", "formalin", "fixed", "nasal", "polyp", "tissues", "from", "47", "subjects", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "cells", "positive", "for", "ER", "or", "PR", "were", "confirmed", "by", "spatial", "location", ",", "dual", "immunolabelling", ",", "and", "histochemical", "staining", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 5, 6, 6, 6, 0, 5, 0, 5, 0, 0, 5, 6, 0, 5, 0, 5, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["RESULTS", ":", "Consistent", "with", "the", "known", "features", "of", "nasal", "polyps", ",", "CD4+", "(", "T", "helper/inducer", ")", ",", "CD8+", "(", "cytotoxic/suppressor", ")", ",", "CD68+", "(", "macrophages", ")", ",", "mast", "cells", ",", "eosinophils", "and", "neutrophils", "were", "all", "clearly", "detected", "by", "their", "relevant", "monoclonal", "antibodies", "or", "appropriate", "histochemical", "staining", ",", "but", "only", "mast", "cells", "tested", "positive", "for", "ER", "/PR", "labelling", "with", "their", "polyclonal", "and", "monoclonal", "antibodies", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["The", "frequencies", "for", "expression", "were", "61.7", "%", "for", "ER", "positive", "and", "59.6", "%", "for", "PR", "positive", "cells", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["The", "expression", "of", "ER", "/", "PR", "was", "independent", "of", "patient", "sex", "and", "age", "but", "was", "highly", "correlated", "with", "the", "numbers", "of", "mast", "cells", "(", "r", "=", "0.973", ",", "p", "<", "0.001", "for", "ER", ";", "r", "=", "0.955", ",", "p", "<", "0.001", "for", "PR", ")", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0], "tokens": ["Fewer", "than", "5", "%", "of", "mast", "cells", "were", "found", "to", "be", "negative", "for", "ER", "/", "PR", "expression", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 5, 0, 5, 0, 0, 0, 5, 6, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0], "tokens": ["CONCLUSIONS", ":", "Mast", "cells", "alone", ",", "but", "not", "lymphocytes", ",", "macrophages", ",", "or", "other", "immune", "cells", ",", "express", "ER", "/", "PR", "in", "human", "upper", "airways", "."]} {"tags": [0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Numerous", "ER/PR", "positive", "mast", "cells", "exist", "in", "nasal", "polyps", ",", "indicating", "that", "this", "may", "be", "a", "major", "route", "for", "the", "involvement", "of", "sex", "hormones", "in", "airway", "inflammation", "when", "exposed", "to", "the", "higher", "and", "varying", "concentration", "of", "oestrogen", "and", "progesterone", "characteristic", "of", "females", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NF", "kappa", "b", "signaling", "in", "posthypoxic", "endothelial", "cells", ":", "relevance", "to", "E-selectin", "expression", "and", "neutrophil", "adhesion", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "previous", "studies", "have", "implicated", "the", "nuclear", "transcription", "factor", "kappa", "B", "(", "NF", "kappa", "B", ")", "in", "the", "regulation", "of", "adhesion", "molecule", "expression", "in", "endothelial", "cells", "exposed", "to", "anoxia-reoxygenation", "(", "A/R", ")", "or", "a", "redox", "imbalance", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0], "tokens": ["The", "objectives", "of", "this", "study", "were", "(", "1", ")", "to", "define", "the", "kinetics", "of", "NF", "kappa", "B", "activation", "by", "examining", "I", "kappa", "B", "alpha", "degradation", "and", "the", "nuclear", "translocation", "of", "p65", "in", "response", "to", "A/R", "or", "redox", "imbalance", "(", "induced", "by", "treatment", "of", "cells", "with", "diamide", "and", "buthionine", "sulfoximine", ")", "and", "(", "2", ")", "to", "determine", "whether", "the", "signal", "for", "I", "kappa", "B", "alpha", "degradation", ",", "nuclear", "translocation", "of", "p65", ",", "and", "E-selectin", "-mediated", "neutrophil", "adhesion", "is", "related", "to", "the", "activity", "of", "protein", "tyrosine", "kinase", "(", "PTK", ")", ",", "protein", "tyrosine", "phosphatase", "(", "PTPase", ")", "and/or", "protein", "kinase", "C", "(", "PKC", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "results", "demonstrate", "that", "both", "A/R", "and", "redox", "imbalance", "led", "to", "I", "kappa", "B", "alpha", "degradation", "within", "30", "min", "and", "the", "concomitant", "appearance", "of", "p65", "in", "the", "nucleus", ",", "consistent", "with", "rapid", "cytosolic", "activation", "of", "NF", "kappa", "B", "and", "subsequent", "nuclear", "translocation", "of", "the", "activated", "p65", "subunit", "."]} {"tags": [0, 0, 3, 0, 3, 4, 4, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Inhibition", "of", "PKC", "blocked", "I", "kappa", "B", "alpha", "degradation", "and", "p65", "translocation", "in", "A/R-challenged", ",", "but", "not", "redox-altered", ",", "endothelial", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["However", ",", "both", "A/R-", "and", "redox-induced", "NF", "kappa", "B", "activation", "was", "blocked", "by", "inhibition", "of", "PTK", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Similarly", ",", "A/R-induced", "E-selectin", "expression", "and", "neutrophil-endothelial", "cell", "adhesion", "were", "blocked", "by", "inhibition", "of", "PKC", "or", "PTK", ",", "while", "only", "PTK", "inhibited", "the", "redox-induced", "adhesion", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Pretreatment", "of", "cells", "with", "N-acetyl", "cysteine", "effectively", "blocked", "A/R-", "or", "redox-induced", "I", "kappa", "B", "degradation", "and", "significantly", "attenuated", "the", "respective", "neutrophil", "adhesion", "responses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Collectively", ",", "these", "findings", "indicate", "that", "A/R-induced", "E-selectin", "expression", "and", "neutrophil-endothelial", "cell", "adhesion", "are", "mediated", "by", "both", "PKC", "and", "PTK", ",", "which", "signal", "rapid", "activation", "of", "NF", "kappa", "B", "."]} {"tags": [0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "A/R-induced", "NF", "kappa", "B", "signaling", "response", "appears", "to", "be", "mediated", ",", "at", "least", "in", "part", ",", "by", "intracellular", "redox", "imbalance", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["Copyright", "2001", "S.", "Karger", "AG", ",", "Basel"]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Induction", "of", "apoptosis", "in", "human", "lymphocytes", "by", "the", "herbicide", "2", ",", "4-dichlorophenoxyacetic", "acid", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Dimethylammonium", "salt", "of", "2", ",", "4-dichlorophenoxyacetic", "acid", "(", "DMA-2", ",", "4-D", ")", "is", "a", "widely", "used", "herbicide", "that", "is", "considered", "moderately", "toxic", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["In", "the", "present", "study", "we", "found", "that", "DMA-2", ",", "4-D", "is", "able", "to", "cause", "apoptosis", "in", "peripheral", "blood", "lymphocytes", "of", "healthy", "individuals", "and", "Jurkat", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Apoptosis", "induced", "by", "DMA-2", ",", "4-D", "was", "dose", "and", "time", "dependent", ",", "independent", "of", "Fas", ",", "TNF", "receptor", "1", "or", "the", "aromatic", "hydrocarbon", "receptor", ",", "and", "involved", "disruption", "of", "the", "mitochondrial", "transmembrane", "potential", "and", "activation", "of", "caspase-9", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["ZVAD-FMK", ",", "a", "broad-spectrum", "inhibitor", "of", "caspases", ",", "blocked", "DMA-2", ",", "4-D-induced", "apoptosis", "completely", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["While", "an", "inhibitor", "of", "caspase-9", ",", "as", "well", "as", "caspase-9", "and", "caspase-3", "inhibitors", "in", "combination", ",", "strongly", "blocked", "DMA-2", ",", "4-D-induced", "apoptosis", ",", "an", "inhibitor", "of", "caspase-3", "had", "a", "moderate", "inhibitory", "effect", "."]} {"tags": [0, 3, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Unlike", "Fas", "-mediated", "apoptosis", ",", "the", "initiator", "caspase", ",", "caspase-8", ",", "was", "not", "involved", "in", "DMA-2", ",", "4-D-induced", "apoptosis", "."]} {"tags": [0, 0, 7, 8, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transfection", "of", "Jurkat", "cells", "with", "Bcl-2", "prevented", "DMA-2", ",", "4-D-induced", "disruption", "of", "the", "mitochondrial", "transmembrane", "potential", "and", "led", "to", "a", "complete", "blockage", "of", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "data", "indicate", "that", "DMA-2", ",", "4-D", "kills", "human", "lymphocytes", "by", "initiating", "apoptosis", "via", "a", "direct", "effect", "on", "mitochondria", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "activation", "of", "caspases", "occurs", "downstream", "of", "mitochondrial", "damage", ",", "and", "the", "dysfunction", "of", "mitochondria", "appears", "to", "be", "sufficient", "for", "triggering", "all", "downstream", "events", "leading", "to", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0], "tokens": ["Inhibition", "of", "Th1", "differentiation", "by", "IL-6", "is", "mediated", "by", "SOCS1", "."]} {"tags": [3, 4, 0, 3, 0, 0, 0, 3, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interleukin", "6", "(", "IL-6", ")", "is", "a", "cytokine", "produced", "by", "immune", "and", "nonimmune", "cells", "and", "exhibits", "functional", "pleiotropy", "and", "redundancy", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["IL-6", "plays", "an", "important", "role", "in", "the", "differentiation", "of", "several", "cell", "types", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Here", ",", "we", "describe", "a", "novel", "function", "of", "IL-6", ":", "the", "negative", "regulation", "of", "CD4", "+", "Th1", "cell", "differentiation", "."]} {"tags": [0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0], "tokens": ["While", "IL-6", "-directed", "CD4", "+", "Th2", "differentiation", "is", "mediated", "by", "IL-4", ",", "inhibition", "of", "Th1", "differentiation", "by", "IL-6", "is", "independent", "of", "IL-4", "."]} {"tags": [3, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0], "tokens": ["IL-6", "upregulates", "suppressor", "of", "cytokine", "signaling", "1", "(", "SOCS1", ")", "expression", "in", "activated", "CD4+", "T", "cells", ",", "thereby", "interfering", "with", "signal", "transducer", "and", "activator", "of", "transcription", "1", "(", "STAT1", ")", "phosphorylation", "induced", "by", "interferon", "gamma", "(", "IFNgamma", ")", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 1, 2, 0, 0, 3, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "IFNgamma", "receptor-mediated", "signals", "by", "IL-6", "prevents", "autoregulation", "of", "IFNgamma", "gene", "expression", "by", "IFNgamma", "during", "CD4+", "T", "cell", "activation", ",", "thereby", "preventing", "Th1", "differentiation", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "IL-6", "promotes", "CD4", "+", "Th2", "differentiation", "and", "inhibits", "Th1", "differentiation", "by", "two", "independent", "molecular", "mechanisms", "."]} {"tags": [0, 0, 3, 4, 4, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Interaction", "between", "CCAAT/enhancer", "binding", "protein", "and", "cyclic", "AMP", "response", "element", "binding", "protein", "1", "regulates", "human", "immunodeficiency", "virus", "type", "1", "transcription", "in", "cells", "of", "the", "monocyte/macrophage", "lineage", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Recent", "observations", "have", "shown", "two", "CCAAT/enhancer", "binding", "protein", "(", "C/EBP", ")", "binding", "sites", "to", "be", "critically", "important", "for", "efficient", "human", "immunodeficiency", "virus", "type", "1", "(", "HIV-1", ")", "replication", "within", "cells", "of", "the", "monocyte/macrophage", "lineage", ",", "a", "cell", "type", "likely", "involved", "in", "transport", "of", "the", "virus", "to", "the", "brain", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 1, 0, 0, 0], "tokens": ["Additionally", ",", "sequence", "variation", "at", "C/EBP", "site", "I", ",", "which", "lies", "immediately", "upstream", "of", "the", "distal", "nuclear", "factor", "kappa", "B", "site", "and", "immediately", "downstream", "of", "a", "binding", "site", "for", "activating", "transcription", "factor", "(", "ATF", ")", "/", "cyclic", "AMP", "response", "element", "binding", "protein", "(", "CREB", ")", ",", "has", "been", "shown", "to", "affect", "HIV-1", "long", "terminal", "repeat", "(", "LTR", ")", "activity", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 3, 4, 0, 0, 1, 2, 2, 0], "tokens": ["Given", "that", "C/EBP", "proteins", "have", "been", "shown", "to", "interact", "with", "many", "other", "transcription", "factors", "including", "members", "of", "the", "ATF/CREB", "family", ",", "we", "proceeded", "to", "determine", "whether", "an", "adjacent", "ATF/CREB", "binding", "site", "could", "affect", "C/EBP", "protein", "binding", "to", "C/EBP", "site", "I", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Electrophoretic", "mobility", "shift", "analyses", "indicated", "that", "selected", "ATF/CREB", "site", "variants", "assisted", "in", "the", "recruitment", "of", "C/EBP", "proteins", "to", "an", "adjacent", ",", "naturally", "occurring", ",", "low-affinity", "C/EBP", "site", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "biophysical", "interaction", "appears", "to", "occur", "via", "at", "least", "two", "mechanisms", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0], "tokens": ["First", ",", "low", "amounts", "of", "CREB-1", "and", "C/EBP", "appear", "to", "heterodimerize", "and", "bind", "to", "a", "site", "consisting", "of", "a", "half", "site", "from", "both", "the", "ATF/CREB", "and", "C/EBP", "binding", "sites", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 1, 2, 0, 0, 3, 4, 0, 0, 0, 1, 2, 2, 0], "tokens": ["In", "addition", ",", "CREB-1", "homodimers", "bind", "to", "the", "ATF/CREB", "site", "and", "recruit", "C/EBP", "dimers", "to", "their", "cognate", "weak", "binding", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 1, 2, 0, 0, 0, 3, 0, 0, 1, 2, 0, 0, 0, 0, 0, 3, 0], "tokens": ["This", "interaction", "is", "reciprocal", ",", "since", "C/EBP", "dimer", "binding", "to", "a", "strong", "C/EBP", "site", "leads", "to", "enhanced", "CREB-1", "recruitment", "to", "ATF/CREB", "sites", "that", "are", "weakly", "bound", "by", "CREB", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Sequence", "variation", "at", "both", "C/EBP", "and", "ATF/CREB", "sites", "affects", "the", "molecular", "interactions", "involved", "in", "mediating", "both", "of", "these", "mechanisms", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Most", "importantly", ",", "sequence", "variation", "at", "the", "ATF/CREB", "binding", "site", "affected", "basal", "LTR", "activity", "as", "well", "as", "LTR", "function", "following", "interleukin-6", "stimulation", ",", "a", "treatment", "that", "leads", "to", "increases", "in", "C/EBP", "activation", "."]} {"tags": [0, 0, 1, 2, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0], "tokens": ["Thus", ",", "HIV-1", "LTR", "ATF/CREB", "binding", "site", "sequence", "variation", "may", "modulate", "cellular", "signaling", "at", "the", "viral", "promoter", "through", "the", "C/EBP", "pathway"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0], "tokens": ["Selective", "inhibition", "of", "interleukin-4", "gene", "expression", "in", "human", "T", "cells", "by", "aspirin", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Previous", "studies", "indicated", "that", "aspirin", "(", "acetylsalicylic", "acid", "[", "ASA", "]", ")", "can", "have", "profound", "immunomodulatory", "effects", "by", "regulating", "cytokine", "gene", "expression", "in", "several", "types", "of", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 0], "tokens": ["This", "study", "is", "the", "first", "in", "which", "concentrations", "of", "ASA", "in", "the", "therapeutic", "range", "were", "found", "to", "significantly", "reduce", "interleukin", "(", "IL", ")", "-4", "secretion", "and", "RNA", "expression", "in", "freshly", "isolated", "and", "mitogen-primed", "human", "CD4+", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0], "tokens": ["In", "contrast", ",", "ASA", "did", "not", "affect", "IL-13", ",", "interferon-gamma", ",", "and", "IL-2", "expression", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["ASA", "inhibited", "IL-4", ",", "but", "not", "IL-2", ",", "promoter-driven", "chloramphenicol", "acetyltransferase", "expression", "in", "transiently", "transfected", "Jurkat", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["The", "structurally", "unrelated", "nonsteroidal", "anti-inflammatory", "drugs", "indomethacin", "and", "flurbiprofen", "did", "not", "affect", "cytokine", "gene", "expression", "in", "T", "cells", ",", "whereas", "the", "weak", "cyclo-oxygenase", "inhibitor", "salicylic", "acid", "was", "at", "least", "as", "effective", "as", "ASA", "in", "inhibiting", "IL-4", "expression", "and", "promoter", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0], "tokens": ["The", "inhibitory", "effect", "of", "ASA", "on", "IL-4", "transcription", "was", "not", "mediated", "by", "decreased", "nuclear", "expression", "of", "the", "known", "salicylate", "target", "nuclear", "factor", "(", "NF", ")", "-kappaB", "and", "was", "accompanied", "by", "reduced", "binding", "of", "an", "inducible", "factor", "to", "an", "IL-4", "promoter", "region", "upstream", "of", ",", "but", "not", "overlapping", ",", "the", "NF", "of", "activated", "T", "cells-", "and", "NF-kappaB-binding", "P1", "element", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["It", "is", "concluded", "that", "anti-inflammatory", "salicylates", ",", "by", "means", "of", "a", "previously", "unrecognized", "mechanism", "of", "action", ",", "can", "influence", "the", "nature", "of", "adaptive", "immune", "responses", "by", "selectively", "inhibiting", "the", "expression", "of", "IL-4", ",", "a", "critical", "effector", "of", "these", "responses", ",", "in", "CD4+", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Molecular", "and", "cellular", "mediators", "of", "interleukin-1", "-dependent", "acute", "inflammatory", "arthritis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 0, 3, 0, 0], "tokens": ["OBJECTIVE", ":", "To", "examine", "the", "molecular", "and", "cellular", "mechanisms", "in", "a", "model", "of", "acute", "inflammatory", "monarticular", "arthritis", "induced", "by", "methylated", "bovine", "serum", "albumin", "(", "mBSA", ")", "and", "interleukin-1", "(", "IL-1", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Mice", "were", "injected", "intraarticularly", "with", "mBSA", "on", "day", "0", "and", "subcutaneously", "with", "recombinant", "human", "IL-1beta", "on", "days", "0-2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["At", "day", "7", ",", "knee", "joints", "were", "removed", "and", "assessed", "histologically", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Flow", "cytometry", "and", "RNase", "protection", "were", "used", "to", "analyze", "IL-1", "-dependent", "events", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "C57BL/6", "(", "B6", ")", ",", "129/Sv", ",", "and", "(", "B6", "x", "129/", "Sv", ")", "F1", "hybrid", "mice", ",", "all", "H-2b", "strains", ",", "were", "susceptible", "to", "mBSA", "/IL-1-induced", "arthritis", ",", "whereas", "C3H/HeJ", "(", "H-2k", ")", "mice", "were", "not", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["B6", "mice", "lacking", "T", "and", "B", "cells", "(", "RAG1-/-", ")", "or", "major", "histocompatibility", "complex", "(", "MHC", ")", "class", "II", "antigens", "(", "MHCII-/-", ")", ",", "and", "B6", "mice", "treated", "with", "a", "CD4+", "T", "cell-depleting", "monoclonal", "antibody", ",", "were", "resistant", "to", "disease", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "B", "cell-deficient", "(", "muMT/", "muMT", ")", "mice", "developed", "arthritis", "at", "an", "incidence", "and", "severity", "similar", "to", "that", "of", "controls", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RelB-deficient", "(", "RelB-/-", ")", "bone", "marrow", "chimeric", "mice", "had", "arthritis", "that", "was", "significantly", "reduced", "in", "incidence", "and", "severity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 3, 3, 4, 4, 4, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "B6", "mice", ",", "flow", "cytometry", "demonstrated", "an", "IL-1-dependent", "leukocyte", "infiltration", "into", "the", "synovial", "compartment", "and", "RNase", "protection", "assays", "revealed", "induction", "of", "messenger", "RNA", "(", "mRNA", ")", "for", "the", "chemokines", "monocyte", "chemoattractant", "protein", "1", ",", "macrophage", "inhibitory", "protein", "2", "(", "MIP-2", ")", ",", "RANTES", ",", "MIP-1alpha", ",", "and", "MIP-1beta", ",", "in", "vivo", "and", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 6, 0, 3, 0], "tokens": ["CONCLUSION", ":", "Arthritis", "induced", "by", "mBSA", "/", "IL-1", "is", "strain", "specific", "and", "dependent", "on", "CD4+", "T", "lymphocytes", "and", "at", "least", "partially", "on", "RelB", ",", "but", "not", "on", "B", "lymphocytes", "or", "antibody", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["IL-1", "contributes", "to", "leukocyte", "recruitment", "to", "the", "synovium", "and", "directly", "induces", "chemokine", "mRNA", "production", "by", "synovial", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["This", "model", "of", "acute", "monarticular", "arthritis", "is", "particularly", "suitable", "for", "further", "investigations", "into", "cell-mediated", "immunity", "in", "arthritis", "and", "the", "role", "of", "IL-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Positive", "and", "negative", "regulation", "of", "granulopoiesis", "by", "endogenous", "RARalpha", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0], "tokens": ["Acute", "promyelocytic", "leukemia", "(", "APL", ")", "is", "always", "associated", "with", "chromosomal", "translocations", "that", "disrupt", "the", "retinoic", "acid", "receptor", "alpha", "(", "RARalpha", ")", "gene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Whether", "these", "translocations", "relate", "to", "a", "role", "for", "endogenous", "RARalpha", "in", "normal", "granulopoiesis", "remains", "uncertain", "because", "most", "studies", "addressing", "this", "question", "have", "used", "non-physiological", "overexpression", "systems", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Granulocyte", "differentiation", "in", "cells", "derived", "from", "RARalpha-deficient", "(", "RARalpha", "(", "-/-", ")", ")", "mice", "was", "studied", "and", "evaluated", "in", "the", "context", "of", "agonist-bound", "and", "ligand-free", "RARalpha", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "results", "demonstrate", "that", "RARalpha", "is", "dispensable", "for", "granulopoiesis", ",", "as", "RARalpha", "(", "-/-", ")", "mice", "have", "a", "normal", "granulocyte", "population", "despite", "an", "impaired", "ability", "to", "respond", "to", "retinoids", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["However", ",", "although", "it", "is", "not", "absolutely", "required", ",", "RARalpha", "can", "bidirectionally", "modulate", "granulopoiesis", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RARalpha", "stimulates", "differentiation", "in", "response", "to", "exogenous", "retinoic", "acid", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "endogenous", "retinoids", "control", "granulopoiesis", "in", "vivo", ",", "as", "either", "vitamin", "A-deficient", "mice", "or", "animals", "treated", "with", "an", "RAR", "antagonist", "accumulate", "more", "immature", "granulocytes", "in", "their", "bone", "marrow", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Conversely", ",", "RARalpha", "acts", "to", "limit", "differentiation", "in", "the", "absence", "of", "ligand", "because", "granulocyte", "precursors", "from", "RARalpha", "(", "-/-", ")", "mice", "differentiate", "earlier", "in", "culture", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 3, 4, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Thus", ",", "the", "block", "in", "granulopoiesis", "exerted", "by", "RARalpha", "fusion", "proteins", "expressed", "in", "APL", "cells", "may", "correspond", "to", "an", "amplification", "of", "a", "normal", "function", "of", "unliganded", "RARalpha", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0], "tokens": ["Expression", "and", "function", "of", "a", "stem", "cell", "promoter", "for", "the", "murine", "CBFalpha2", "gene", ":", "distinct", "roles", "and", "regulation", "in", "natural", "killer", "and", "T", "cell", "development", "."]} {"tags": [0, 3, 4, 4, 4, 3, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "Runt", "family", "transcription", "factor", "CBFalpha2", "(", "AML1", ",", "PEBP2alphaB", ",", "or", "Runx1", ")", "is", "required", "by", "hematopoietic", "stem", "cells", "and", "expressed", "at", "high", "levels", "in", "T-lineage", "cells", "."]} {"tags": [0, 5, 6, 6, 3, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 0, 0, 0, 5, 6, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "human", "T", "cells", "CBFalpha2", "is", "usually", "transcribed", "from", "a", "different", "promoter", "(", "distal", "promoter", ")", "than", "in", "myeloid", "cells", "(", "proximal", "promoter", ")", ",", "but", "the", "developmental", "and", "functional", "significance", "of", "this", "promoter", "switch", "has", "not", "been", "known", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Here", ",", "we", "report", "that", "both", "coding", "and", "noncoding", "sequences", "of", "the", "distal", "5", "'", "end", "are", "highly", "conserved", "between", "the", "human", "and", "the", "murine", "genes", ",", "and", "the", "distal", "promoter", "is", "responsible", "for", "the", "overwhelming", "majority", "of", "CBFalpha2", "expression", "in", "murine", "hematopoietic", "stem", "cells", "as", "well", "as", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Distal", "promoter", "activity", "is", "maintained", "throughout", "T", "cell", "development", "and", "at", "lower", "levels", "in", "B", "cell", "development", ",", "but", "downregulated", "in", "natural", "killer", "cell", "development", "."]} {"tags": [0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["The", "distal", "N-terminal", "isoform", "binds", "to", "functionally", "important", "regulatory", "sites", "from", "known", "target", "genes", "with", "two-", "to", "threefold", "higher", "affinity", "than", "the", "proximal", "N-terminal", "isoform", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["Neither", "full-length", "isoform", "alters", "growth", "of", "a", "myeloid", "cell", "line", "under", "nondifferentiating", "conditions", ",", "but", "the", "proximal", "isoform", "selectively", "delays", "mitotic", "arrest", "of", "the", "cell", "line", "under", "differentiating", "conditions", ",", "resulting", "in", "the", "generation", "of", "greater", "numbers", "of", "neutrophils", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Constitutive", "PI3-K", "activity", "is", "essential", "for", "proliferation", ",", "but", "not", "survival", ",", "of", "Theileria", "parva-transformed", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Theileria", "is", "an", "intracellular", "parasite", "that", "causes", "lymphoproliferative", "disorders", "in", "cattle", ",", "and", "infection", "of", "leucocytes", "induces", "a", "transformed", "phenotype", "similar", "to", "tumour", "cells", ",", "but", "the", "mechanisms", "by", "which", "the", "parasite", "induces", "this", "phenotype", "are", "not", "understood", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", ",", "we", "show", "that", "infected", "B", "lymphocytes", "display", "constitutive", "phosphoinositide", "3-kinase", "(", "PI3-K", ")", "activity", ",", "which", "appears", "to", "be", "necessary", "for", "proliferation", ",", "but", "not", "survival", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Importantly", ",", "we", "demonstrate", "that", "one", "mechanism", "by", "which", "PI3-K", "mediates", "the", "proliferation", "of", "infected", "B", "lymphocytes", "is", "through", "the", "induction", "of", "a", "granulocyte-monocyte", "colony-stimulating", "factor", "(", "GM-CSF", ")", "-dependent", "autocrine", "loop", "."]} {"tags": [3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 5, 0], "tokens": ["PI3-K", "induction", "of", "GM-CSF", "appears", "to", "be", "at", "the", "transcriptional", "level", "and", ",", "consistently", ",", "we", "demonstrate", "that", "PI3-K", "is", "also", "involved", "in", "the", "constitutive", "induction", "of", "AP-1", "and", "NF-kappaB", ",", "which", "characterizes", "Theileria-infected", "leucocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Taken", "together", ",", "our", "results", "highlight", "a", "novel", "strategy", "exploited", "by", "the", "intracellular", "parasite", "Theileria", "to", "induce", "continued", "proliferation", "of", "its", "host", "leucocyte", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Lymphokine", "dependence", "of", "STAT3", "activation", "produced", "by", "surface", "immunoglobulin", "cross-linking", "and", "by", "phorbol", "ester", "plus", "calcium", "ionophore", "treatment", "in", "B", "cells", "."]} {"tags": [0, 0, 5, 6, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Stimulation", "of", "B", "cells", "by", "surface", "immunoglobulin", "(", "sIg", ")", "triggering", ",", "or", "through", "the", "mitogenic", "combination", "of", "phorbol", "ester", "and", "calcium", "ionophore", ",", "is", "accompanied", "by", "activation", "of", "STAT", "transcription", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "mechanism", "responsible", "for", "the", "delayed", "nuclear", "accumulation", "of", "phosphorylated", "STAT3", "was", "examined", "in", "detail", ",", "focusing", "on", "the", "role", "of", "B", "cell-derived", "lymphokines", "."]} {"tags": [3, 0, 0, 0, 3, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0], "tokens": ["sIg", "-induced", "activation", "of", "STAT3", "was", "partially", "inhibited", "in", "B", "cells", "obtained", "from", "IL-6-", "or", "IL-10-deficient", "mice", ",", "and", "was", "partially", "blocked", "by", "neutralizing", "antibodies", "directed", "against", "either", "of", "these", "lymphokines", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["sIg", "-induced", "STAT3", "activation", "was", "completely", "inhibited", "by", "combining", "IL-6-", "and", "IL-10-specific", "neutralizing", "antibodies", ",", "or", "by", "adding", "individual", "neutralizing", "antibodies", "to", "B", "cells", "obtained", "from", "lymphokine-deficient", "animals", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "IL-10", "alone", "appeared", "to", "account", "for", "STAT3", "activation", "resulting", "from", "B", "cell", "stimulation", "with", "phorbol", "ester", "and", "calcium", "ionophore", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["In", "keeping", "with", "these", "results", ",", "soluble", "IL-6", "and", "IL-10", "were", "found", "in", "supernatant", "fluid", "obtained", "from", "stimulated", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["This", "work", "indicates", "that", "a", "lymphokine", "pathway", "is", "responsible", "for", "STAT3", "activation", "that", "occurs", "late", "after", "B", "cell", "stimulation", ",", "and", "points", "out", "differences", "in", "B", "cell", "activation", "that", "result", "from", "stimulation", "through", "the", "antigen", "receptor", "and", "through", "pharmacological", "mimicry", "of", "signaling", "mediators", "."]} {"tags": [3, 4, 4, 0, 0, 3, 0, 1, 2, 2, 2, 0], "tokens": ["Transcription", "factor", "AP-4", "is", "a", "ligand", "for", "immunoglobulin-kappa", "promoter", "E-box", "elements", "."]} {"tags": [1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Immunoglobulin", "(", "Ig", ")", "-kappa", "promoters", "from", "humans", "and", "mice", "share", "conserved", "sequences", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0], "tokens": ["The", "octamer", "element", "is", "common", "to", "all", "Ig", "promoters", "and", "pivotal", "for", "their", "function", "."]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "other", "conserved", "sequence", "motifs", ",", "that", "differ", "between", "Ig", "variable", "gene", "families", ",", "are", "required", "for", "normal", "promoter", "function", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0], "tokens": ["These", "conserved", "motifs", "do", "not", "stimulate", "transcription", "in", "the", "absence", "of", "an", "octamer", "."]} {"tags": [0, 0, 0, 0, 1, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["One", "example", "is", "an", "E-box", "of", "the", "E47/E12", "type", "(", "5'-CAGCTG-3", "'", ")", ",", "which", "is", "found", "in", "all", "promoters", "of", "the", "human", "and", "murine", "Ig-kappa", "gene", "subgroups/families", ",", "with", "the", "exception", "of", "subgroups", "II", "and", "VI", "and", "their", "related", "murine", "families", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0], "tokens": ["In", "the", "present", "study", "we", "show", "that", "the", "ubiquitously", "expressed", "transcription", "factor", "AP-4", ",", "and", "not", "E47", ",", "interacts", "specifically", "with", "the", "kappa", "promoter", "E-boxes", "when", "tested", "in", "electrophoretic", "mobility-shift", "assays", "using", "nuclear", "extracts", "derived", "from", "human", "and", "murine", "B-cell", "lines", "."]} {"tags": [0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "AP-4", ",", "unlike", "E47", ",", "did", "not", "act", "as", "a", "transactivator", ",", "which", "is", "in", "agreement", "with", "previous", "studies", "on", "intact", "kappa", "promoters", ",", "showing", "that", "transcription", "is", "absent", "when", "the", "octamer", "element", "has", "been", "mutated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 3, 0, 0, 3, 4, 0, 1, 2, 2, 0], "tokens": ["Based", "on", "these", "data", ",", "and", "the", "conservation", "of", "the", "5'-CAGCTG-3", "'", "motif", "among", "human", "and", "murine", "kappa", "promoters", ",", "we", "propose", "that", "AP-4", "is", "the", "major", "ligand", "for", "Ig-kappa", "promoter", "E-boxes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "16-mer", "peptide", "(", "RQIKIWFQNRRMKWKK", ")", "from", "antennapedia", "preferentially", "targets", "the", "Class", "I", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Translocation", "of", "antigenic", "peptides", "into", "the", "cytosol", "of", "antigen", "presenting", "cells", "facilitates", "proteosomal", "processing", "and", "loading", "into", "Class", "I", "molecules", "for", "MHC", "presentation", "on", "the", "cell", "surface", "."]} {"tags": [0, 3, 4, 4, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["The", "DNA", "binding", "domain", "of", "the", "Drosophila", "transcription", "factor", "(", "Antennapedia", ")", ",", "a", "60", "amino", "acid", "protein", ",", "is", "rapidly", "taken", "up", "by", "cells", "and", "has", "been", "fused", "to", "selected", "antigens", "to", "enhance", "their", "immunogenicity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "now", "demonstrate", "that", "a", "16", "amino", "acid", "peptide", "from", "antennapedia", "can", "facilitate", "the", "cytoplasmic", "uptake", "of", "CTL", "epitope", "9-mer", "peptides", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Synthetic", "peptides", "were", "made", "containing", "the", "16-mer", "antennapedia", "peptide", "linked", "in", "tandem", "to", "the", "ovalbumin", "SIINFEKL", "CTL", "peptide", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["The", "peptide", "complex", "was", "shown", "to", "rapidly", "internalise", "into", "APCs", "by", "confocal", "microscopy", "."]} {"tags": [0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 7, 0, 0], "tokens": ["This", "peptide", "induced", "CTL", "in", "C57BL/6", "mice", "and", "protected", "them", "against", "growth", "of", "an", "ovalbumin", "expressing", "tumour", "cell", "line", "(", "E.G7-OVA", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0], "tokens": ["The", "ability", "of", "the", "hybrid", "peptide", "to", "be", "processed", "and", "presented", "by", "APCs", "was", "similar", ",", "whether", "the", "SIINFEKL", "sequence", "was", "appended", "at", "the", "C-terminus", "or", "N-terminus", "of", "the", "Antennapedia", "peptide", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0], "tokens": ["The", "production", "of", "synthetic", "peptides", "containing", "other", "CTL", "peptide", "epitopes", "may", "be", "useful", "for", "priming", "CTLs", "in", "vitro", "and", "in", "vivo"]} {"tags": [5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Bone", "marrow", "cells", "promote", "TH2", "polarization", "and", "inhibit", "virus-specific", "CTL", "generation", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 5, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["This", "laboratory", "recently", "reported", "that", "human", "bone", "marrow", "cells", "(", "BMC", ")", "inhibit", "the", "generation", "of", "virus-specific", "CTL", "in", "culture", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "culture", "supernatants", "contained", "increased", "levels", "of", "prostaglandin", "E", "(", "2", ")", "(", "PGE", "(", "2", ")", ")", "(", "shown", "to", "favor", "TH2", "cell", "development", ")", "and", "also", "inhibited", "EBV-CTL", "effector", "cell", "development", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", ",", "we", "obtained", "PBL", "from", "Epstein-Barr", "virus", "(", "EBV", ")", "IgG", "antibody", "positive", "kidney", "transplant", "recipients", "(", "R", ")", "and", "their", "living-related", "donors", "(", "LRD", ")", "one", "year", "after", "renal", "transplantation", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 5, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["EBV-specific", "CTL", "were", "then", "generated", "in", "vitro", "by", "stimulating", "PBL", "with", "autologous", "EBV-transformed", "B", "cells", "(", "EBV-B", ")", "in", "the", "presence", "or", "absence", "of", "autologous", "BMC", "."]} {"tags": [0, 0, 0, 5, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 5, 6, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0], "tokens": ["The", "addition", "of", "BMC", "to", "the", "EBV-CTL", "generation", "cultures", "increased", "the", "intracellular", "expression", "in", "CD3+", "cells", "of", "IL-4", ",", "-5", ",", "-6", ",", "-10", ",", "and", "-13", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["These", "CD3+", "cells", "also", "expressed", "increased", "levels", "of", "the", "TH2", "associated", "receptor", "CCR3", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 5, 6, 0, 0, 5, 0, 5, 0], "tokens": ["Inhibition", "was", "even", "observed", "by", "preparing", "EBV-CTL", "generating", "cultures", "in", "trans-wells", "that", "separated", "the", "autologous", "BMC", "from", "the", "PBL", "+", "EBV-B", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "was", "then", "observed", "that", "CD3+", "cells", "obtained", "after", "7", "days", "of", "culture", "in", "the", "presence", "of", "autologous", "BMC", "could", "be", "used", "as", "inhibitors", "of", "EBV-CTL", "generation", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 5, 0], "tokens": ["Protein", "Kinase", "A", "(", "PKA", ")", ",", "a", "cAMP", "kinase", "that", "is", "involved", "in", "the", "upregulation", "of", "TH2", "cytokine", "activity", ",", "was", "increased", "in", "EBV-CTL", "cultures", "by", "the", "presence", "of", "BMC", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Additionally", ",", "IL-4-mediated", "signal", "transduction", "and", "activation", "of", "transcription", "(", "STAT-6", ")", "phosphorylation", "was", "slightly", "increased", "."]} {"tags": [0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "results", "show", "that", "the", "BMC", "inhibition", "is", "mediated", "by", "soluble", "factors", "(", "cytokines", ")", "and", "that", "cell-cell", "contact", "in", "this", "autologous", "system", "is", "not", "required", ",", "so", "that", "BMC", "(", "at", "least", "partially", ",", "via", "cytokine", "production", ")", "promote", "TH2", "polarization", "in", "culture", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 5, 0], "tokens": ["Moreover", ",", "TH2", "cells", "induced", "by", "culturing", "with", "autologous", "BMC", "directly", "inhibit", "EBV-CTL", "generation", ",", "and", "TH2", "associated", "PKA", ",", "CCR3", ",", "and", "STAT-6", "phosphorylation", "are", "enhanced", "by", "BMC", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Reduction", "in", "DNA", "binding", "activity", "of", "the", "transcription", "factor", "Pax-5a", "in", "B", "lymphocytes", "of", "aged", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Aging", "has", "been", "associated", "with", "intrinsic", "changes", "of", "the", "humoral", "immune", "response", ",", "which", "may", "lead", "to", "an", "increased", "occurrence", "of", "autoimmune", "disorders", "and", "pathogenic", "susceptibility", "."]} {"tags": [0, 3, 4, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "transcription", "factor", "Pax-5", "is", "a", "key", "regulator", "of", "B", "cell", "development", "."]} {"tags": [3, 4, 4, 4, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0], "tokens": ["Pax-5a/B", "cell-specific", "activator", "protein", "and", "an", "alternatively", "spliced", "isoform", ",", "Pax-5d", ",", "may", "have", "opposing", "functions", "in", "transcriptional", "regulation", "due", "to", "the", "lack", "of", "a", "transactivation", "domain", "in", "Pax-5d", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["To", "study", "B", "cell", "-specific", "changes", "that", "occur", "during", "the", "aging", "process", ",", "we", "investigated", "expression", "patterns", "of", "Pax-5a", "and", "5d", "in", "mature", "B", "cells", "of", "young", "and", "aged", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["RNase", "protection", "assays", "showed", "a", "similar", "transcriptional", "pattern", "for", "both", "age", "groups", "that", "indicates", "that", "aging", "has", "no", "affect", "on", "transcription", "initiation", "or", "alternative", "splicing", "for", "either", "isoform", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "a", "significant", "reduction", "in", "the", "DNA", "binding", "activity", "of", "Pax-5a", "but", "not", "Pax-5d", "protein", "was", "observed", "in", "aged", "B", "cells", "in", "vitro", ",", "while", "Western", "blot", "analyses", "showed", "that", "similar", "levels", "of", "Pax-5a", "and", "5d", "proteins", "were", "present", "in", "both", "age", "groups", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 5, 6, 6, 0], "tokens": ["The", "observed", "decrease", "in", "Pax-5a", "binding", "activity", "correlated", "with", "changes", "in", "expression", "of", "two", "Pax-5", "target", "genes", "in", "aged", "B", "cells", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Expression", "of", "the", "Ig", "J", "chain", "and", "the", "secreted", "form", "of", "Ig", "mu", ",", "which", "are", "both", "known", "to", "be", "suppressed", "by", "Pax-5a", "in", "mature", "B", "cells", ",", "were", "increased", "in", "B", "cells", "of", "aged", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3], "tokens": ["Together", ",", "our", "studies", "suggest", "that", "changes", "associated", "with", "the", "aging", "phenotype", "cause", "posttranslational", "modification", "(", "s", ")", "of", "Pax-5a", "but", "not", "Pax-5d", ",", "which", "may", "lead", "to", "an", "abnormal", "B", "cell", "phenotype", "in", "aged", "mice", ",", "associated", "with", "elevated", "levels", "of", "J", "chain", ",", "and", "secretion", "of", "IgM"]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["NF-kappa", "B/Rel", "participation", "in", "the", "lymphokine-dependent", "proliferation", "of", "T", "lymphoid", "cells", "."]} {"tags": [0, 0, 0, 5, 6, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Proliferative", "responses", "of", "lymphoid", "cells", "to", "IL-2", "and", "IL-4", "depend", "on", "activation", "of", "the", "cells", ",", "but", "the", "mechanism", "(", "s", ")", "by", "which", "activation", "enhances", "cellular", "competence", "to", "respond", "to", "cytokines", "is", "not", "fully", "understood", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "NF-kappaB/Rel", "family", "represents", "one", "signal", "transduction", "pathway", "induced", "during", "such", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0], "tokens": ["We", "show", "in", "this", "study", "that", "inhibition", "of", "NF-kappaB", "through", "the", "expression", "of", "an", "IkappaBalpha", "(", "inhibitory", "protein", "that", "dissociates", "from", "NF-kappaB", ")", "mutant", "refractory", "to", "signal-induced", "degradation", "(", "IkappaBalpha", "(", "DeltaN", ")", ")", "interfered", "with", "the", "acquisition", "of", "competence", "to", "proliferate", "in", "response", "to", "IL-4", "as", "well", "as", "IL-2", "."]} {"tags": [5, 0, 5, 6, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Thymocytes", "and", "T", "cells", "from", "IkappaBalpha", "(", "DeltaN", ")", "transgenic", "mice", "expressed", "normal", "levels", "of", "IL-2R", "subunits", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["However", ",", "transgenic", "cells", "exhibited", "a", "dramatic", "defect", "in", "Stat5A", "activation", "treatment", "with", "IL-2", ",", "and", "a", "similar", "defect", "was", "observed", "for", "IL-4", "-induced", "Stat5", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "T", "lymphoid", "cells", "with", "inhibition", "of", "NF-kappaB", "showed", "normal", "insulin", "receptor", "substrate-2", "phosphorylation", "and", "only", "a", "modest", "decrease", "in", "Stat6", "activation", "and", "insulin", "receptor", "substrate-1", "phosphorylation", "after", "IL-4", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["These", "results", "indicate", "that", "the", "NF-kappaB/Rel/IkappaBalpha", "system", "can", "regulate", "cytokine", "receptor", "capacitation", "through", "effects", "on", "the", "induction", "of", "downstream", "signaling", "by", "the", "Stat", "transcription", "factor", "family", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BMS-189453", ",", "a", "novel", "retinoid", "receptor", "antagonist", ",", "is", "a", "potent", "testicular", "toxin", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0], "tokens": ["BMS-189453", "is", "a", "synthetic", "retinoid", "that", "acts", "as", "an", "antagonist", "at", "retinoic", "acid", "receptors", "alpha", ",", "beta", ",", "and", "gamma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 3, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "Sprague", "Dawley", "rats", "at", "daily", "oral", "doses", "of", "15", ",", "60", ",", "or", "240", "mg/kg", "for", "1", "month", ",", "BMS-189453", "produced", "increases", "in", "leukocyte", "counts", ",", "alkaline", "phosphatase", "and", "alanine", "aminotransferase", "levels", ",", "and", "marked", "testicular", "degeneration", "and", "atrophy", "at", "all", "doses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Significant", "overt", "signs", "of", "toxicity", "and", "deaths", "occurred", "at", "240", "mg/kg", ",", "whereas", "body-weight", "and", "food-consumption", "decreases", "occurred", "at", "60", "and", "240", "mg/kg", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["When", "BMS-189453", "was", "administered", "to", "male", "rats", "at", "daily", "doses", "ranging", "from", "12.5", "to", "100", "mg/kg", "for", "1", "week", ",", "only", "minimal", "testicular", "changes", "occurred", "at", "all", "doses", ",", "shortly", "after", "the", "dosing", "period", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "after", "a", "1-month", "drug-free", "observation", "period", ",", "marked", "testicular", "atrophy", "was", "evident", "at", "all", "doses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BMS-189453", "was", "then", "administered", "at", "doses", "of", "2", ",", "10", ",", "or", "50", "mg/kg", "to", "male", "rats", "for", "1", ",", "3", ",", "or", "7", "consecutive", "days", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Dose-", "and", "duration-dependent", "testicular", "toxicity", "that", "occurred", "after", "a", "1-month", "observation", "period", "did", "not", "recover", ",", "and", ",", "in", "some", "cases", ",", "was", "more", "severe", "4", "months", "after", "the", "last", "dose", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "rabbits", "administered", "BMS-189453", "at", "oral", "doses", "of", "2", ",", "10", ",", "or", "50", "mg/kg", "for", "1", "week", ",", "testicular", "degeneration", "and", "atrophy", "were", "evident", "in", "the", "high-dose", "group", "at", "1", "month", "following", "treatment", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "studies", "indicate", "that", "retinoid", "antagonists", "can", "selectively", "produce", "progressive", "and", "prolonged", "testicular", "toxicity", "after", "single", "or", "repeated", "oral", "doses", "that", "are", "otherwise", "well", "tolerated", "."]} {"tags": [0, 0, 0, 3, 0, 3, 3, 4, 0, 0, 0, 0], "tokens": ["Sequential", "involvement", "of", "NFAT", "and", "Egr", "transcription", "factors", "in", "FasL", "regulation", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0], "tokens": ["The", "critical", "function", "of", "NFAT", "proteins", "in", "maintaining", "lymphoid", "homeostasis", "was", "revealed", "in", "mice", "lacking", "both", "NFATp", "and", "NFAT4", "(", "DKO", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["DKO", "mice", "exhibit", "increased", "lymphoproliferation", ",", "decreased", "activation-induced", "cell", "death", ",", "and", "impaired", "induction", "of", "FasL", "."]} {"tags": [0, 3, 4, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "transcription", "factors", "Egr2", "and", "Egr3", "are", "potent", "activators", "of", "FasL", "expression", "."]} {"tags": [0, 0, 0, 0, 1, 0, 1, 0, 1, 2, 2, 0], "tokens": ["Here", "we", "find", "that", "Egr2", "and", "Egr3", "are", "NFAT", "target", "genes", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Activation", "of", "FasL", "occurs", "via", "the", "NFAT", "-dependent", "induction", "of", "Egr3", ",", "as", "demonstrated", "by", "the", "ability", "of", "exogenously", "provided", "NFATp", "to", "restore", "Egr", "-dependent", "FasL", "promoter", "activity", "in", "DKO", "lymph", "node", "cells", "."]} {"tags": [0, 0, 1, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Further", ",", "Egr3", "expression", "is", "enriched", "in", "Th1", "cells", ",", "suggesting", "a", "molecular", "basis", "for", "the", "known", "preferential", "expression", "of", "FasL", "in", "the", "Th1", "versus", "Th2", "subset", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mechanisms", "and", "clinical", "relevance", "of", "nongenomic", "glucocorticoid", "actions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoids", "have", "profound", "anti-inflammatory", "and", "immunosuppressive", "actions", "when", "used", "therapeutically", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "therapeutic", "dose", "is", "quite", "variable", "and", "depends", "on", "the", "disease", ",", "but", "ranges", "from", "very", "low", "to", "extremely", "high", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "rationale", "for", "the", "use", "of", "various", "dosage", "regimens", "for", "specific", "clinical", "indications", "is", "the", "existence", "of", "three", "distinct", ",", "therapeutically", "relevant", "effects", ":", "genomic", ",", "specific", "nongenomic", "and", "unspecific", "nongenomic", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Genomic", "effects", "are", "mediated", "by", "cytosolic", "receptors", "that", "alter", "expression", "of", "specific", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Specific", "nongenomic", "effects", "occur", "within", "a", "few", "minutes", "and", "are", "mediated", "by", "steroid-selective", "membrane", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Unspecific", "nongenomic", "effects", "occur", "within", "seconds", ",", "but", "only", "at", "high", "glucocorticoid", "dosages", ",", "and", "seem", "to", "result", "from", "direct", "interactions", "with", "biological", "membranes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["For", "unspecific", "nongenomic", "effects", ",", "methylprednisolone", "and", "other", "glucocorticoids", "have", "been", "shown", "to", "inhibit", "cation", "cycling", "across", "the", "plasma", "membrane", ",", "but", "to", "have", "little", "effect", "on", "protein", "synthesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "glucocorticoids", "could", "diminish", "or", "prevent", "the", "acute", "immune", "response", "by", "interfering", "with", "processes", "such", "as", "the", "rise", "in", "intracellular", "Ca2+", "concentration", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "is", "proposed", "that", "the", "additional", "therapeutic", "benefit", "of", "higher", "doses", "is", "obtained", "via", "these", "nongenomic", "effects", "."]} {"tags": [0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0], "tokens": ["High", "glucose-induced", "intercellular", "adhesion", "molecule-1", "(", "ICAM-1", ")", "expression", "through", "an", "osmotic", "effect", "in", "rat", "mesangial", "cells", "is", "PKC-NF-kappa", "B-dependent", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["AIMS/HYPOTHESIS", ":", "Infiltration", "of", "mononuclear", "cells", "and", "glomerular", "enlargement", "accompanied", "by", "glomerular", "cell", "proliferation", "are", "very", "early", "characteristics", "of", "the", "pathophysiology", "of", "diabetes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0], "tokens": ["To", "clarify", "the", "mechanism", "of", "early", "diabetic", "nephropathy", ",", "we", "measured", "[", "3H", "]", "-thymidine", "incorporation", "and", "cell", "numbers", "to", "show", "the", "influence", "of", "a", "high", "ambient", "glucose", "concentration", "and", "the", "osmotic", "effect", "on", "rat", "mesangial", "cell", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 5, 0, 5, 6, 0], "tokens": ["We", "also", "measured", "the", "effect", "of", "high", "glucose", "on", "the", "expression", "of", "intercellular", "adhesion", "molecule-1", "and", "vascular", "adhesion", "molecule-1", "by", "flow", "cytometry", "and", "semiquantitative", "RT-PCR", "in", "mesangial", "cells", "and", "the", "adhesion", "of", "leukocytes", "to", "mesangial", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 5, 6, 0], "tokens": ["METHODS/RESULTS", ":", "Cells", "exposed", "to", "high", "D-glucose", "(", "30", "mmol/l", ")", "caused", "an", "increase", "in", "[", "3H", "]", "-thymidine", "incorporation", "and", "cell", "numbers", "at", "24", "and", "48", "h", "and", "normalized", "at", "72", "h", "(", "p", "<", "0.05", ")", ",", "whereas", "these", "changes", "were", "not", "found", "in", "high", "mannitol", "(", "30", "mmol/l", ")", ",", "IL-1", "beta", ",", "or", "TNF", "alpha", "-stimulated", "mesangial", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cells", "exposed", "to", "high-glucose", "(", "15", ",", "30", ",", "or", "60", "mmol/l", ")", "or", "osmotic", "agents", "(", "L-glucose", ",", "raffinose", "and", "mannitol", ")", "showed", "that", "intercellular", "adhesion", "molecule-1", "expression", "began", "to", "increase", "after", "24", "h", ",", "reached", "its", "maximum", "at", "24", "and", "48", "h", "and", "gradually", "decreased", "afterwards", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "stimulatory", "effects", "of", "high", "glucose", "and", "high", "mannitol", "on", "mRNA", "expression", "were", "observed", "as", "early", "as", "6", "h", "and", "reached", "its", "maximum", "at", "12", "h", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 5, 6, 0], "tokens": ["Up-regulation", "of", "ICAM-1", "protein", "and", "mRNA", "was", "also", "found", "in", "IL-1-beta", "and", "TNF-alpha", "-stimulated", "mesangial", "cells", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Neither", "vascular", "adhesion", "molecule-1", "protein", "nor", "mRNA", "expression", "was", ",", "however", ",", "affected", "by", "high", "glucose", "and", "high", "mannitol", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Notably", ",", "the", "protein", "kinase", "C", "inhibitors", "calphostin", "C", "and", "staurosporine", "reduced", "high", "glucose-", "or", "high", "mannitol-induced", "intercellular", "adhesion", "molecule-1", "mRNA", "expression", "and", "high", "glucose-induced", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "the", "NF-kappa", "B", "inhibitor", "N-tosyl-L-phenylalanine", "chloromethyl", "ketone", "reduced", "high", "glucose-", "or", "high", "mannitol-induced", "intercellular", "adhesion", "molecule-1", "mRNA", "expression", "and", "high", "glucose-induced", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Results", "showed", "that", "high", "glucose", "(", "15", ",", "30", "mmol/l", ")", "or", "high", "concentrations", "of", "osmotic", "agents", "remarkably", "increased", "the", "number", "of", "adherent", "leukocytes", "to", "mesangial", "cells", "(", "p", "<", "0.01", ")", "compared", "with", "control", "cells", "(", "5", "mmol/l", "D-glucose", ")", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 5, 6, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Functional", "blocking", "of", "intercellular", "adhesion", "molecule-1", "on", "mesangial", "cells", "with", "rat", "intercellular", "adhesion", "molecule-1", "monoclonal", "antibody", ",", "calphostin", "C", ",", "staurosporine", ",", "or", "N-tosyl-L-phenylalanine", "chloromethyl", "ketone", "significantly", "inhibited", "high", "glucose-", "or", "high", "mannitol-induced", "increase", "in", "leukocyte", "adhesion", "(", "p", "<", "<", "0.05", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 3, 4, 4, 0, 3, 0, 3, 4, 0, 0, 0], "tokens": ["CONCLUSION/INTERPRETATION", ":", "These", "results", "suggest", "that", "high", "glucose", "can", "upregulate", "intercellular", "adhesion", "molecule-1", "protein", "and", "mRNA", "expression", "but", "not", "vascular", "adhesion", "molecule-1", "expression", "in", "mesangial", "cells", "and", "promote", "leukocyte", "adhesion", "through", "up-regulation", "of", "intercellular", "adhesion", "molecule-1", "through", "osmotic", "effect", ",", "possibly", "depending", "on", "the", "protein", "kinase", "C", "nuclear", "factor-kappa", "B", "(", "PKC", "-", "NF-kappa", "B", ")", "pathway", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["High", "glucose", "itself", "can", "also", "promote", "mesangial", "cell", "proliferation", "through", "the", "PKC-NF-kappa", "B", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "conclude", "that", "hyperglycaemia", "in", "itself", "seems", "to", "be", "an", "important", "factor", "in", "the", "development", "of", "early", "diabetic", "nephropathy", "."]} {"tags": [0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 0, 0, 3, 0, 0], "tokens": ["Combined", "corticosteroid/", "granulocyte", "colony-stimulating", "factor", "(", "G-CSF", ")", "therapy", "in", "the", "treatment", "of", "severe", "congenital", "neutropenia", "unresponsive", "to", "G-CSF", ":", "Activated", "glucocorticoid", "receptors", "synergize", "with", "G-CSF", "signals", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0], "tokens": ["More", "than", "90", "%", "of", "patients", "with", "severe", "congenital", "neutropenia", "(", "SCN", ")", "respond", "to", "granulocyte", "colony-stimulating", "factor", "(", "G-CSF", ")", "therapy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "basis", "for", "the", "refractory", "state", "in", "the", "remaining", "patients", "is", "unknown", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 3, 0, 0], "tokens": ["To", "address", "this", "issue", ",", "we", "studied", "a", "child", "with", "SCN", "who", "was", "totally", "unresponsive", "to", "G-CSF", "and", "had", "a", "novel", "point", "mutation", "in", "the", "extracellular", "domain", "of", "the", "G-CSF", "receptor", "(", "GCSF-R", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["Marrow", "stromal", "support", "of", "granulopoiesis", "was", "evaluated", "by", "plating", "CD34", "(", "+", ")", "cells", "on", "preformed", "stromal", "layers", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nonadherent", "cells", "were", "harvested", "and", "assayed", "in", "clonogenic", "assays", "for", "granulocytic", "colony", "production", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "in", "vitro", "effect", "of", "G-CSF", "and", "corticosteroids", "on", "granulopoiesis", "was", "evaluated", "in", "clonogenic", "assays", "of", "marrow", "mononuclear", "cells", ",", "by", "proliferation", "studies", "of", "the", "murine", "myeloid", "cell", "line", "32D", "expressing", "the", "patient", "'s", "mutated", "G-CSFR", ",", "and", "by", "measuring", "STAT5", "activation", "in", "nuclear", "extracts", "from", "stimulated", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["Patient", "'s", "stroma", "supported", "granulopoiesis", "derived", "from", "control", "marrow", "CD34", "(", "+", ")", "cells", "in", "a", "normal", "manner", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["Normal", "stroma", ",", "however", ",", "failed", "to", "induce", "granulopoiesis", "from", "patient", "'s", "CD34", "(", "+", ")", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Clonogenic", "assays", "of", "the", "patient", "'s", "marrow", "mononuclear", "cells", "incorporating", "either", "G-CSF", "or", "hydrocortisone", "produced", "little", "neutrophil", "growth", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "inclusion", "of", "both", "G-CSF", "and", "hydrocortisone", "in", "the", "cytokine", "``", "cocktail", "''", "markedly", "increased", "the", "neutrophil", "numbers", "."]} {"tags": [0, 0, 7, 8, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Proliferation", "of", "32D", "cells", "expressing", "the", "mutated", "receptor", "and", "STAT5", "activation", "were", "improved", "by", "a", "combination", "of", "G-CSF", "and", "dexamethasone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["When", "small", "daily", "doses", "of", "oral", "prednisone", "were", "then", "administered", "to", "the", "patient", "with", "conventional", "doses", "of", "subcutaneous", "G-CSF", ",", "the", "patient", "responded", "with", "increased", "neutrophil", "numbers", "and", "with", "a", "complete", "reversal", "of", "the", "infectious", "problems", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["These", "data", "provide", "insight", "into", "SCN", "unresponsive", "to", "standard", "G-CSF", "treatment", "and", "to", "the", "potential", "corrective", "action", "of", "combined", "treatment", "with", "G-CSF", "and", "corticosteroids", "through", "synergistic", "activation", "of", "STAT5", "."]} {"tags": [5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Human", "renal", "mesangial", "cells", "are", "a", "target", "for", "the", "anti-inflammatory", "action", "of", "9-cis", "retinoic", "acid", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Mesangial", "cells", "play", "an", "active", "role", "in", "the", "inflammatory", "response", "to", "glomerular", "injury", "."]} {"tags": [0, 0, 0, 0, 7, 8, 8, 8, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0], "tokens": ["We", "have", "studied", "in", "cultured", "human", "mesangial", "cells", "(", "CHMC", ")", "several", "effects", "of", "9-cis", "retinoic", "acid", "(", "9-cRA", ")", ",", "an", "activator", "of", "both", "retinoic", "acid", "receptors", "(", "RARs", ")", "and", "retinoid", "X", "receptors", "(", "RXRs", ")", "."]} {"tags": [0, 0, 0, 0, 0, 7, 0, 0], "tokens": ["9-cRA", "inhibited", "foetal", "calf", "serum-induced", "CHMC", "proliferation", "."]} {"tags": [0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "also", "prevented", "CHMC", "death", "induced", "by", "the", "inflammatory", "mediator", "H", "(", "2", ")", "O", "(", "2", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "preventive", "effect", "was", "not", "due", "to", "any", "increase", "in", "H", "(", "2", ")", "O", "(", "2", ")", "catabolism", "and", "it", "persisted", "even", "when", "both", "catalase", "and", "glutathione", "synthesis", "were", "inhibited", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["Finally", ",", "9-cRA", "diminished", "monocyte", "adhesion", "to", "FCS-stimulated", "CHMC", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 0], "tokens": ["Interestingly", ",", "the", "retinoid", "also", "inhibited", "in", "FCS-stimulated", "cells", "the", "protein", "expression", "of", "two", "mesangial", "adhesion", "molecules", ",", "fibronectin", "and", "osteopontin", ",", "but", "it", "did", "not", "modify", "the", "protein", "expression", "of", "intercellular", "adhesion", "molecule-1", "and", "vascular", "adhesion", "molecule-1", "."]} {"tags": [0, 0, 3, 4, 4, 4, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["All", "major", "RARs", "and", "RXRs", "isotypes", "were", "expressed", "in", "CHMC", "regardless", "of", "the", "presence", "or", "absence", "of", "9-cRA", "."]} {"tags": [0, 0, 9, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0], "tokens": ["Transcripts", "to", "RAR-alpha", ",", "RAR-beta", "and", "RXR-alpha", "increased", "after", "incubation", "with", "9-cRA", "whereas", "RXR-gamma", "was", "inhibited", ",", "suggesting", "a", "major", "role", "for", "RARs", "and", "RXRs", "in", "9-cRA-anti-inflammatory", "effects", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["9-cRA", "was", "toxic", "only", "at", "50", "microM", "(", "a", "concentration", "50", "-", "5000", "times", "higher", "than", "required", "for", "the", "effects", "above", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cell", "death", "occurred", "by", "apoptosis", ",", "whose", "onset", "was", "associated", "with", "a", "pronounced", "increase", "in", "catalase", "activity", "and", "reduced", "glutathione", "content", ",", "being", "more", "effectively", "induced", "by", "all-trans", "retinoic", "acid", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Modulation", "of", "the", "oxidant/antioxidant", "balance", "failed", "to", "inhibit", "apoptosis", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "conclude", "that", "mesangial", "cells", "might", "be", "a", "target", "for", "the", "treatment", "of", "inflammatory", "glomerulopathies", "with", "9-cRA", "."]} {"tags": [0, 0, 1, 2, 2, 0], "tokens": ["Expression", "of", "mammalian", "defensin", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Antimicrobial", "peptides", "are", "a", "prevalent", "mechanism", "of", "host", "defense", "found", "throughout", "nature", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["In", "mammals", ",", "defensins", "are", "among", "the", "most", "abundant", "of", "these", "broad-spectrum", "antibiotics", ",", "and", "are", "expressed", "in", "epithelial", "and", "hematopoietic", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "defensin", "peptides", "are", "especially", "abundant", "in", "neutrophils", ";", "however", ",", "gene", "expression", "is", "limited", "to", "the", "promyelocyte", "stage", "."]} {"tags": [0, 5, 6, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "epithelial", "cells", ",", "defensin", "genes", "are", "found", "as", "both", "constitutively", "expressed", "and", "inducible", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Induction", "has", "been", "observed", "in", "vitro", "by", "stimulation", "with", "bacterial", "lipopolysaccharide", "as", "well", "as", "inflammatory", "mediators", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "vivo", ",", "up-regulation", "of", "several", "defensin", "genes", "occurs", "in", "both", "infectious", "and", "inflammatory", "states", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 4, 0], "tokens": ["Gene", "regulation", "occurs", "via", "signal", "transduction", "pathways", "common", "to", "other", "innate", "immune", "responses", ",", "utilizing", "transcription", "factors", "such", "as", "nuclear", "factor", "(", "NF", ")", "-kappaB", "and", "NF", "interleukin-6", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Together", ",", "the", "data", "suggest", "a", "broad-based", "innate", "host", "defense", "whereby", "potent", "antimicrobial", "peptides", "are", "present", "to", "prevent", "initial", "colonization", "by", "pathogenic", "microorganisms", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "the", "recognition", "of", "bacteria", "coupled", "with", "a", "nascent", "inflammatory", "response", "can", "bolster", "this", "defense", "by", "a", "coordinated", "up-regulation", "of", "the", "peptides", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Multiple", "signals", "required", "for", "cyclic", "AMP-responsive", "element", "binding", "protein", "(", "CREB", ")", "binding", "protein", "interaction", "induced", "by", "CD3/CD28", "costimulation", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["The", "optimal", "activation", "of", "cAMP-responsive", "element", "binding", "protein", "(", "CREB", ")", ",", "similar", "to", "the", "full", "activation", "of", "T", "lymphocytes", ",", "requires", "the", "stimulation", "of", "both", "CD3", "and", "CD28", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Using", "a", "reporter", "system", "to", "detect", "interaction", "of", "CREB", "and", "CREB-binding", "protein", "(", "CBP", ")", ",", "in", "this", "study", "we", "found", "that", "CREB", "binds", "to", "CBP", "only", "by", "engagement", "of", "both", "CD3", "and", "CD28", "."]} {"tags": [3, 0, 3, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CD3/CD28", "-promoted", "CREB", "-", "CBP", "interaction", "was", "dependent", "on", "p38", "mitogen-activated", "protein", "kinase", "(", "MAPK", ")", "and", "calcium/calmodulin-dependent", "protein", "kinase", "(", "CaMK", ")", "IV", "in", "addition", "to", "the", "previously", "identified", "extracellular", "signal-regulated", "kinase", "pathway", "."]} {"tags": [3, 4, 4, 4, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Extracellular", "signal-regulated", "kinase", ",", "CaMKIV", ",", "and", "p38", "MAPK", "were", "also", "the", "kinases", "involved", "in", "CREB", "Ser", "(", "133", ")", "phosphorylation", "induced", "by", "CD3/CD28", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["A", "reconstitution", "experiment", "illustrated", "that", "optimum", "CREB", "-", "CBP", "interaction", "and", "CREB", "trans-activation", "were", "attained", "when", "these", "three", "kinase", "pathways", "were", "simultaneously", "activated", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Our", "results", "demonstrate", "that", "coordinated", "activation", "of", "different", "kinases", "leads", "to", "full", "activation", "of", "CREB", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0], "tokens": ["Notably", ",", "CD28", "ligation", "activated", "p38", "MAPK", "and", "CaMKIV", ",", "the", "kinases", "stimulated", "by", "CD3", "engagement", ",", "suggesting", "that", "CD28", "acts", "by", "increasing", "the", "activation", "extent", "of", "p38", "MAPK", "and", "CaMKIV", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0], "tokens": ["These", "results", "support", "the", "model", "of", "a", "minimum", "activation", "threshold", "for", "CREB", "-", "CBP", "interaction", "that", "can", "be", "reached", "only", "when", "both", "CD3", "and", "CD28", "are", "stimulated", "."]} {"tags": [0, 1, 2, 2, 0, 1, 2, 2, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["The", "murine", "IL-2", "promoter", "contains", "distal", "regulatory", "elements", "responsive", "to", "the", "Ah", "receptor", ",", "a", "member", "of", "the", "evolutionarily", "conserved", "bHLH-PAS", "transcription", "factor", "family", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 6, 0], "tokens": ["Signaling", "through", "the", "TCR", "and", "costimulatory", "signals", "primarily", "control", "transcription", "of", "the", "IL-2", "gene", "in", "naive", "T", "cells", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["The", "minimal", "promoter", "necessary", "for", "this", "expression", "lies", "proximal", ",", "between", "-300", "and", "the", "transcription", "start", "site", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 9, 0, 5, 6, 6, 0], "tokens": ["We", "had", "previously", "shown", "that", "activation", "of", "the", "arylhydrocarbon", "receptor", "(", "AHR", ")", ",", "a", "member", "of", "the", "bHLH-PAS", "family", "of", "transcription", "factors", ",", "leads", "to", "increased", "mRNA", "expression", "of", "IL-2", "in", "murine", "fetal", "thymocytes", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "AHR", "is", "abundant", "in", "the", "thymus", "and", "may", "play", "a", "role", "for", "the", "development", "of", "the", "immune", "system", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Moreover", ",", "its", "overactivation", "by", "chemicals", "such", "as", "dioxins", "leads", "to", "immunosuppression", "and", "thymic", "involution", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 1, 2, 2, 0], "tokens": ["Binding", "motifs", "for", "the", "liganded", "AHR", "can", "be", "identified", "in", "the", "distal", "region", "-1300", "to", "-800", "of", "the", "mouse", "IL-2", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "show", "here", "that", "these", "DNA", "motifs", ",", "the", "so-called", "dioxin", "response", "elements", ",", "after", "binding", "to", "the", "liganded", "AHR", "are", "sufficient", "to", "transactivate", "luciferase", "expression", "in", "a", "reporter", "gene", "system", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 0, 3, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["The", "IL-2", "gene", "can", "be", "induced", "by", "the", "AHR", "also", "in", "thymocytes", "in", "vivo", "after", "injection", "of", "2", ",", "3", ",", "7", ",", "8-tetrachlorodibenzo-p-dioxin", ",", "a", "potent", "ligand", "of", "the", "AHR", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["The", "AHR", "mediates", "the", "IL-2", "induction", "as", "shown", "with", "AHR", "-deficient", "mice", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 1, 2, 0, 0], "tokens": ["However", ",", "in", "spleen", "cells", "in", "vitro", "costimulation", "via", "the", "TCR", "is", "necessary", "for", "optimal", "IL-2", "gene", "induction", "."]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 3, 0, 0, 1, 0, 0, 0, 0, 0, 1, 2], "tokens": ["Thus", ",", "the", "IL-2", "promoter", "region", "contains", "novel", "distal", "regulatory", "elements", "that", "can", "be", "addressed", "by", "the", "AHR", "to", "induce", "IL-2", "and", "can", "cooperate", "with", "the", "proximal", "promoter"]} {"tags": [0, 0, 3, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Visualization", "of", "Syk-antigen", "receptor", "interactions", "using", "green", "fluorescent", "protein", ":", "differential", "roles", "for", "Syk", "and", "Lyn", "in", "the", "regulation", "of", "receptor", "capping", "and", "internalization", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["The", "cross-linking", "of", "the", "B", "cell", "Ag", "receptor", "(", "BCR", ")", "is", "coupled", "to", "the", "stimulation", "of", "multiple", "intracellular", "signal", "transduction", "cascades", "via", "receptor-associated", ",", "protein", "tyrosine", "kinases", "of", "both", "the", "Src", "and", "Syk", "families", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0, 7, 8, 8, 0, 3, 4, 0, 0, 3, 0, 0, 3, 4, 4, 0], "tokens": ["To", "monitor", "changes", "in", "the", "subcellular", "distribution", "of", "Syk", "in", "B", "cells", "responding", "to", "BCR", "cross-linking", ",", "we", "expressed", "in", "Syk", "-deficient", "DT40", "B", "cells", "a", "fusion", "protein", "consisting", "of", "Syk", "coupled", "to", "green", "fluorescent", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Treatment", "of", "these", "cells", "with", "anti-IgM", "Abs", "leads", "to", "the", "recruitment", "of", "the", "kinase", "from", "cytoplasmic", "and", "nuclear", "compartments", "to", "the", "site", "of", "the", "cross-linked", "receptor", "at", "the", "plasma", "membrane", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "Syk-receptor", "complexes", "aggregate", "into", "membrane", "patches", "that", "redistribute", "to", "form", "a", "cap", "at", "one", "pole", "of", "the", "cell", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Syk", "is", "not", "demonstrably", "associated", "with", "the", "internalized", "receptor", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0], "tokens": ["Catalytically", "active", "Syk", "promotes", "and", "stabilizes", "the", "formation", "of", "tightly", "capped", "BCR", "complexes", "at", "the", "plasma", "membrane", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Lyn", "is", "not", "required", "for", "the", "recruitment", "of", "Syk", "to", "the", "cross-linked", "receptor", ",", "but", "is", "required", "for", "the", "internalization", "of", "the", "clustered", "BCR", "complexes", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0], "tokens": ["In", "the", "absence", "of", "Lyn", ",", "receptor-Syk", "complexes", "at", "the", "plasma", "membrane", "are", "long", "lived", ",", "and", "the", "receptor-mediated", "activation", "of", "the", "NF-AT", "transcription", "factor", "is", "enhanced", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "Lyn", "appears", "to", "function", "to", "negatively", "regulate", "aspects", "of", "BCR", "-dependent", "signaling", "by", "stimulating", "receptor", "internalization", "and", "down-regulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0], "tokens": ["Renal", "interstitial", "fibrosis", "is", "reduced", "in", "angiotensin", "II", "type", "1a", "receptor", "-deficient", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Unilateral", "ureteral", "obstruction", "(", "UUO", ")", "results", "in", "tubulointerstitial", "fibrosis", "of", "the", "affected", "kidney", "by", "stimulating", "the", "renin-angiotensin", "system", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "study", "established", "a", "UUO", "model", "in", "angiotensin", "type", "1a", "receptor", "(", "AT1a", ")", "deficient", "(", "mutant", ")", "mice", "to", "elucidate", "the", "role", "of", "angiotensin", "II", "through", "AT1a", "on", "the", "fibrosis", "of", "the", "obstructed", "kidney", "(", "OBK", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 5, 0, 5, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["The", "relative", "volume", "of", "the", "tubulointerstitium", "was", "measured", "by", "an", "image", "analyzer", ";", "deposition", "of", "collagen", "types", "III", "and", "IV", "and", "monocyte", "/", "macrophage", "infiltration", "were", "histologically", "examined", "using", "specific", "antibodies", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0], "tokens": ["Also", "determined", "were", "the", "mRNA", "levels", "of", "transforming", "growth", "factor-beta", "by", "Northern", "blot", "analysis", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nuclear", "factor-kappaB", "activity", "was", "assessed", "by", "gel", "shift", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 5, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["UUO", "in", "wild", "mice", "resulted", "in", "a", "marked", "expansion", "of", "relative", "volume", "of", "the", "tubulointerstitium", ",", "together", "with", "increased", "deposition", "of", "collagen", "types", "III", "and", "IV", "and", "number", "of", "infiltrated", "monocytes", "/", "macrophages", "in", "the", "interstitium", ",", "relative", "to", "sham-operated", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "comparison", ",", "these", "changes", "were", "significantly", "lower", "in", "mutant", "mice", "with", "UUO", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "mRNA", "level", "of", "transforming", "growth", "factor-beta", "was", "significantly", "higher", "in", "the", "OBK", "of", "wild", "mice", "with", "UUO", "compared", "with", "sham-operated", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "the", "increase", "in", "mRNA", "level", "in", "the", "OBK", "of", "mutant", "mice", "was", "significantly", "less", "than", "in", "wild", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Finally", ",", "UUO", "resulted", "in", "activation", "of", "nuclear", "factor-kappaB", "in", "wild", "mice", "but", "was", "inhibited", "in", "the", "OBK", "of", "mutant", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "results", "provide", "direct", "evidence", "that", "angiotensin", "II", "acting", "via", "the", "AT1a", "plays", "a", "pivotal", "role", "in", "the", "development", "of", "tubulointerstitial", "fibrosis", "in", "UUO", "."]} {"tags": [3, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["HLA-DQ", "tetramers", "identify", "epitope-specific", "T", "cells", "in", "peripheral", "blood", "of", "herpes", "simplex", "virus", "type", "2-infected", "individuals", ":", "direct", "detection", "of", "immunodominant", "antigen-responsive", "cells", "."]} {"tags": [5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ag-specific", "CD4+", "T", "cells", "are", "present", "in", "peripheral", "blood", "in", "low", "frequency", ",", "where", "they", "undergo", "recruitment", "and", "expansion", "during", "immune", "responses", "and", "in", "the", "pathogenesis", "of", "numerous", "autoimmune", "diseases", "."]} {"tags": [3, 4, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["MHC", "tetramers", ",", "which", "constitute", "a", "labeled", "MHC-peptide", "ligand", "suitable", "for", "binding", "to", "the", "Ag-specific", "receptor", "on", "T", "cells", ",", "provide", "a", "novel", "approach", "for", "the", "detection", "and", "characterization", "of", "such", "rare", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", ",", "we", "utilized", "this", "technology", "to", "identify", "HLA", "DQ", "-restricted", "Ag-specific", "T", "cells", "in", "the", "peripheral", "blood", "of", "human", "subjects", "and", "to", "identify", "immunodominant", "epitopes", "associated", "with", "viral", "infection", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Peptides", "representing", "potential", "epitope", "regions", "of", "the", "VP16", "protein", "from", "HSV-2", "were", "loaded", "onto", "recombinant", "DQ0602", "molecules", "to", "generate", "a", "panel", "of", "Ag-specific", "DQ0602", "tetramers", "."]} {"tags": [3, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["VP16", "Ag", "-specific", "DQ-restricted", "T", "cells", "were", "identified", "and", "expanded", "from", "the", "peripheral", "blood", "of", "HSV-2-infected", "individuals", ",", "representing", "two", "predominant", "epitope", "specificities", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "the", "VP16", "369-380", "peptide", "has", "a", "lower", "binding", "affinity", "for", "DQ0602", "molecules", "than", "the", "VP16", "33-52", "peptide", ",", "T", "cells", "that", "recognized", "the", "VP16", "369-380", "peptide", "occurred", "at", "a", "much", "higher", "frequency", "than", "those", "that", "were", "specific", "for", "the", "VP16", "33-52", "peptide", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Down-regulation", "of", "BOB.1/OBF.1", "and", "Oct2", "in", "classical", "Hodgkin", "disease", "but", "not", "in", "lymphocyte", "predominant", "Hodgkin", "disease", "correlates", "with", "immunoglobulin", "transcription", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["In", "contrast", "to", "the", "tumor", "cells", "(", "L", "&", "H", "cells", ")", "of", "lymphocyte", "predominant", "Hodgkin", "disease", "(", "LPHD", ")", ",", "Hodgkin", "and", "Reed-Sternberg", "(", "HRS", ")", "cells", "of", "classical", "Hodgkin", "disease", "(", "cHD", ")", "are", "unable", "to", "transcribe", "immunoglobulin", ",", "despite", "the", "presence", "of", "rearranged", "immunoglobulin", "genes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0], "tokens": ["Although", "initial", "studies", "have", "suggested", "crippling", "immunoglobulin", "gene", "mutations", "to", "be", "the", "cause", "of", "absent", "immunoglobulin", "expression", "in", "cHD", ",", "recent", "work", "of", "our", "group", "has", "demonstrated", "an", "impaired", "activation", "of", "the", "immunoglobulin", "promoter", "as", "a", "superior", "mechanism", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["As", "immunoglobulin", "transcription", "is", "mainly", "regulated", "by", "the", "B-cell", "transcription", "factors", "Oct2", "and", "BOB.1/OBF.1", ",", "we", "analyzed", "35", "cases", "of", "LPHD", ",", "32", "cases", "of", "cHD", ",", "and", "2", "Hodgkin", "disease", "cell", "lines", "for", "the", "expression", "of", "these", "transcription", "factors", "and", "also", "in", "parallel", "for", "immunoglobulin", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Our", "results", "demonstrate", "an", "absence", "of", "Oct2", "and/or", "BOB.1/OBF.1", "in", "cHD", "and", "a", "striking", "overexpression", "of", "Oct2", "in", "LPHD", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Immunoglobulin", "expression", "was", "lacking", "in", "cHD", "but", "present", "in", "LPHD", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 5, 6, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Furthermore", ",", "the", "reintroduction", "of", "BOB.1/OBF.1", "and", "Oct2", "into", "cultured", "HRS", "cells", "restored", "the", "activity", "of", "cotransduced", "immunoglobulin", "promoter", "constructs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Our", "findings", "dismiss", "the", "concept", "that", "the", "different", "immunoglobulin", "expression", "in", "cHD", "and", "LPHD", "is", "due", "to", "disrupting", "mutations", "of", "immunoglobulin", "V", "genes", "in", "cHD", "but", "is", "most", "likely", "due", "to", "a", "down-regulation", "of", "Oct2", "and/or", "BOB.1/OBF.1", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 5, 6, 0], "tokens": ["This", "study", "further", "revealed", "Oct2", "as", "a", "new", "and", "valuable", "marker", "for", "the", "identification", "of", "L", "&", "H", "cells", "and", "their", "distinction", "from", "HRS", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "impairment", "of", "immunoglobulin", "transcription", "with", "a", "down-regulated", "synthesis", "of", "Oct2", "and", "BOB.1/OBF.1", "is", "the", "first", "established", "general", "recurrent", "defect", "found", "in", "HRS", "cells", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Constitutive", "expression", "of", "NF-kappa", "B", "is", "a", "characteristic", "feature", "of", "mycosis", "fungoides", ":", "implications", "for", "apoptosis", "resistance", "and", "pathogenesis", "."]} {"tags": [0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "NF-kappa", "B", "family", "of", "transcription", "factors", "is", "an", "important", "regulator", "of", "genes", "expressed", "during", "inflammatory", "responses", ",", "immunoglobulin", "(", "Ig", ")", "class", "switching", ",", "cellular", "differentiation", ",", "and", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Recently", ",", "members", "of", "the", "NF-kappaB", "family", ",", "including", "p65", "(", "Rel", "A", ")", ",", "have", "been", "implicated", "in", "promoting", "survival", "of", "various", "hematopoeitic", "neoplasms", ",", "including", "T", "cell", "malignancies", "such", "as", "adult", "T", "cell", "leukemia-lymphoma", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 0], "tokens": ["We", "investigated", "the", "expression", "of", "active", "NF-kappa", "B", "p65", "(", "Rel", "A", ")", "in", "cases", "of", "mycosis", "fungoides", "(", "MF", ")", "and", "the", "effect", "of", "chemical", "inhibitors", "of", "NF-kappa", "B", "on", "apoptosis", "in", "cutaneous", "T", "cell", "lymphoma", "(", "CTCL", ")", "cell", "lines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["Paraffin-embedded", "tissues", "from", "23", "cutaneous", "lesions", "and", "a", "single", "lymph", "node", "biopsy", "from", "patients", "diagnosed", "with", "MF", "were", "evaluated", "for", "p65", "(", "Rel", "A", ")", "expression", "by", "using", "a", "monoclonal", "mouse", "antibody", "that", "detects", "the", "activated", "form", "of", "p65", "(", "Rel", "A", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Apoptosis", "after", "treatment", "with", "the", "NF-kappa", "B", "inhibitors", "gliotoxin", ",", "MG132", ",", "BAY", "11-7082", ",", "and", "BAY", "11-7085", "was", "quantitatively", "measured", "in", "the", "CTCL", "cell", "lines", "HuT-78", "and", "HH", "by", "propidium", "iodide", "(", "PI", ")", "/cell", "cycle", "analysis", "for", "detection", "of", "a", "hypodiploid", "(", "sub-G", "(", "0", ")", ")", "population", "and", "by", "determination", "of", "increased", "Annexin", "V/7-amino-actinomycin", "D", "(", "7-AAD", ")", "expression", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nuclear", "extracts", "from", "CTCL", "cells", "before", "and", "after", "chemical", "inhibition", "were", "analyzed", "for", "NF-kappa", "B", "nuclear", "DNA-binding", "activity", "by", "electrophoretic", "mobility", "shift", "assay", "(", "EMSA", ")", "with", "quantitative", "densitometry", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Nuclear", "expression", "of", "p65", "(", "Rel", "A", ")", "before", "and", "after", "treatment", "with", "the", "various", "inhibitory", "compounds", "was", "measured", "by", "immunofluorescence", "staining", "in", "each", "CTCL", "cell", "line", "."]} {"tags": [5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0], "tokens": ["Neoplastic", "T", "lymphocytes", "from", "22", "of", "24", "cases", "of", "MF", "showed", "strong", "nuclear", "and", "cytoplasmic", "expression", "of", "active", "p65", "(", "Rel", "A", ")", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0], "tokens": ["Compared", "with", "untreated", "control", "cells", ",", "a", "marked", "increase", "in", "apoptosis", ",", "a", "significant", "decrease", "in", "NF-kappa", "B", "DNA-binding", "activity", ",", "and", "a", "marked", "decrease", "in", "nuclear", "p65", "(", "Rel", "A", ")", "expression", "were", "seen", "in", "cells", "from", "both", "CTCL", "cell", "lines", "after", "chemical", "NF-kappa", "B", "inhibition", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0], "tokens": ["These", "data", "show", "that", "the", "active", "form", "of", "NF-kappa", "B", "p65", "(", "Rel", "A", ")", "is", "commonly", "expressed", "in", "neoplastic", "T", "lymphocytes", "in", "patients", "with", "MF", "."]} {"tags": [0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "CTCL", "cell", "lines", ",", "the", "significant", "decrease", "in", "nuclear", "NF-kappa", "B", "expression", "and", "the", "marked", "increase", "in", "spontaneous", "apoptosis", "caused", "by", "chemical", "NF-kappa", "B", "inhibition", "suggest", "a", "critical", "role", "for", "NF-kappa", "B", "in", "the", "pathogenesis", "and", "tumor", "cell", "maintenance", "of", "CTCLs", "."]} {"tags": [0, 0, 0, 0, 0, 0], "tokens": ["HUM", "PATHOL", "31", ":", "1482-1490", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Human", "T-cell", "leukemia", "virus", "type", "1", "tax", "protein", "activates", "transcription", "through", "AP-1", "site", "by", "inducing", "DNA", "binding", "activity", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Human", "T-cell", "leukemia", "virus", "type", "1", "(", "HTLV-1", ")", "Tax", "protein", "induces", "the", "expression", "of", "various", "family", "members", "of", "the", "transcription", "factor", "AP-1", ",", "such", "as", "c-Jun", ",", "JunD", ",", "c-Fos", ",", "and", "Fra-1", ",", "at", "the", "level", "of", "RNA", "expression", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 1, 2, 0, 1, 2, 0, 0, 5, 6, 0], "tokens": ["We", "examined", "the", "activity", "of", "Tax", "in", "transcription", "through", "AP-1-binding", "sites", "(", "AP-1", "site", ")", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 7, 8, 8, 8, 0], "tokens": ["Transient", "transfection", "studies", "showed", "that", "Tax", "activated", "the", "expression", "of", "a", "luciferase", "gene", "regulated", "by", "two", "copies", "of", "an", "AP-1", "site", "in", "the", "human", "Jurkat", "T-cell", "line", "."]} {"tags": [3, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 1, 2, 0], "tokens": ["Tax", "activates", "the", "expression", "of", "viral", "and", "cellular", "genes", "through", "two", "different", "enhancers", ":", "a", "cAMP-responsive", "(", "CRE", ")", "-like", "element", "and", "a", "kappaB", "element", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Two", "Tax", "mutants", "differentially", "activated", "expression", "of", "these", "two", "elements", "."]} {"tags": [3, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Tax703", "preferentially", "activated", "the", "kappaB", "element", "but", "not", "the", "CRE-like", "one", ",", "whereas", "TaxM22", "showed", "the", "reverse", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "Tax703", "and", "Tax", ",", "but", "not", "TaxM22", ",", "converted", "cell", "growth", "of", "a", "mouse", "T-cell", "line", "from", "being", "interleukin", "(", "IL", ")", "-2-dependent", "to", "being", "IL-2-independent", "."]} {"tags": [0, 0, 3, 4, 0, 3, 0, 3, 0, 0, 0, 0, 1, 2, 0, 0, 7, 8, 0], "tokens": ["Unlike", "the", "wild-type", "Tax", ",", "Tax703", "and", "TaxM22", "only", "weakly", "activated", "the", "AP-1", "site", "in", "the", "T-cell", "line", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "Tax", "seems", "to", "activate", "the", "AP-1", "site", "via", "mechanisms", "distinct", "from", "those", "of", "kappaB", "or", "CRE-like", "elements", ",", "and", "the", "activation", "of", "the", "AP-1", "site", "is", "dispensable", "for", "IL-2-independent", "growth", "of", "CTLL-2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Electrophoretic", "mobility", "shift", "assays", "showed", "that", "Tax", "induced", "strong", "binding", "activity", "to", "an", "AP-1", "site", "in", "CTLL-2", ",", "whereas", "Tax703", "did", "not", ",", "indicating", "that", "the", "induction", "of", "binding", "activity", "to", "the", "AP-1", "site", "is", "essential", "for", "the", "transcriptional", "activation", "by", "Tax", "."]} {"tags": [0, 3, 4, 0, 0, 3, 0, 0, 0, 3, 0, 3, 0], "tokens": ["The", "binding", "complex", "induced", "by", "Tax", "in", "CTLL-2", "contained", "JunD", "and", "Fra-2", "."]} {"tags": [0, 3, 4, 0, 0, 0], "tokens": ["Other", "AP-1", "proteins", "were", "undetectable", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 7, 8, 0, 3, 0, 3, 0, 0, 0], "tokens": ["Activation", "of", "transcription", "through", "the", "AP-1", "site", "in", "Jurkat", "cells", "by", "JunD", "and/or", "Fra-2", "was", "weak", "."]} {"tags": [3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["c-Jun", ",", "JunB", ",", "and", "c-Fos", "activation", "was", "greater", ",", "although", "the", "level", "was", "still", "less", "than", "that", "with", "Tax", "."]} {"tags": [0, 0, 0, 0, 0, 9, 10, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 3, 0], "tokens": ["Thus", ",", "the", "induction", "of", "AP-1", "mRNA", "by", "Tax", "may", "not", "be", "sufficient", "for", "a", "complete", "activation", "of", "AP-1", "site", "by", "Tax", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 1, 2, 0, 1, 2, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["Our", "results", "suggest", "that", "Tax", "activates", "the", "transcription", "of", "cellular", "genes", "with", "AP-1", "sites", "by", "inducing", "the", "DNA-binding", "activity", "of", "AP-1", "proteins", "in", "T", "cells", ",", "a", "mechanism", "distinct", "from", "those", "of", "CRE-like", "and", "kappaB", "elements", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Copyright", "2001", "Academic", "Press", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Strict", "control", "of", "human", "immunodeficiency", "virus", "type", "1", "replication", "by", "a", "genetic", "switch", ":", "Tet", "for", "Tat", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Live-attenuated", "human", "immunodeficiency", "virus", "type", "1", "(", "HIV-1", ")", "variants", "have", "shown", "great", "promise", "as", "AIDS", "vaccines", ",", "but", "continued", "replication", "can", "lead", "to", "the", "selection", "of", "faster-replicating", "variants", "that", "are", "pathogenic", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "therefore", "designed", "HIV-1", "genomes", "that", "replicate", "exclusively", "upon", "addition", "of", "the", "nontoxic", "effector", "doxycycline", "(", "dox", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "was", "achieved", "by", "replacement", "of", "the", "viral", "TAR-Tat", "system", "for", "transcriptional", "activation", "by", "the", "Escherichia", "coli-derived", "Tet", "system", "for", "inducible", "gene", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "designer", "``", "HIV-rtTA", "''", "viruses", "replicate", "in", "a", "strictly", "dox-dependent", "manner", "both", "in", "a", "T-cell", "line", "and", "in", "primary", "blood", "cells", ",", "and", "the", "rate", "of", "replication", "can", "be", "fine-tuned", "by", "simple", "variation", "of", "the", "dox", "concentration", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "HIV-rtTA", "viruses", "provide", "a", "tool", "to", "perform", "genetics", ",", "e.g.", ",", "selection", "and", "optimization", "experiments", ",", "with", "the", "E.", "coli-derived", "Tet", "reagents", "in", "a", "eukaryotic", "background", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "such", "viruses", "may", "represent", "improved", "vaccine", "candidates", "because", "their", "replication", "can", "be", "turned", "on", "and", "off", "at", "will", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Synovial", "fluid", "induced", "nuclear", "factor-kappaB", "DNA", "binding", "in", "a", "monocytic", "cell", "line", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 4, 0, 3, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0], "tokens": ["OBJECTIVE", ":", "To", "determine", "the", "effects", "of", "synovial", "fluids", "(", "SF", ")", "on", "DNA", "binding", "activity", "of", "transcription", "factor", "nuclear", "factor-kappaB", "(", "NF-kappaB", ")", "in", "the", "Mono", "Mac", "6", "monocytic/macrophage", "cell", "line", "as", "a", "model", "for", "the", "interaction", "between", "SF", "and", "synovial", "tissue", "macrophages", "in", "arthritis", "."]} {"tags": [0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Mono", "Mac", "6", "cells", "were", "incubated", "with", "SF", "from", "the", "knee", "joints", "of", "human", "subjects", "with", "rheumatoid", "arthritis", "(", "RA", ")", ",", "undifferentiated", "seronegative", "oligoarthritis", ",", "and", "osteoarthritis", "(", "OA", ")", "."]} {"tags": [0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Nuclear", "extracts", "prepared", "from", "the", "Mono", "Mac", "6", "cells", "and", "RA", "synovial", "tissue", "were", "analyzed", "by", "electrophoretic", "mobility", "shift", "analysis", "(", "EMSA", ")", "for", "NF-kappaB", "DNA", "binding", "proteins", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "Induction", "of", "NF-kappaB", "DNA", "binding", "by", "the", "p65", "(", "RelA", ")", "/p50", "heterodimer", "was", "observed", "in", "response", "to", "incubation", "of", "Mono", "Mac", "6", "cells", "with", "SF", "(", "20", "%", "in", "culture", "medium", ")", "from", "5", "of", "8", "subjects", "with", "RA", ",", "4", "of", "5", "with", "OA", ",", "and", "none", "of", "3", "with", "undifferentiated", "seronegative", "oligoarthritis", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 3, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Incubation", "of", "SF", "with", "neutralizing", "antibodies", "against", "tumor", "necrosis", "factor-alpha", "(", "TNF-alpha", ")", ",", "but", "not", "antibodies", "against", "interleukin", "6", "(", "IL-6", ")", ",", "significantly", "reduced", "the", "induction", "of", "p65/p50", "binding", "activity", "in", "SF", "from", "subjects", "with", "RA", "and", "OA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Unexpectedly", ",", "a", "slowly", "migrating", "SF", "inducible", "NF-kappaB", "-binding", "complex", "was", "observed", "in", "EMSA", "of", "Mono", "Mac", "6", "cells", "after", "incubation", "with", "SF", "from", "5", "of", "8", "RA", "and", "2", "of", "5", "OA", "subjects", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 3, 0, 3, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "induction", "of", "this", "complex", "by", "SF", "was", "not", "affected", "by", "neutralization", "of", "TNF-alpha", "or", "IL-6", "in", "SF", ",", "and", "the", "complex", "was", "not", "inducible", "by", "TNF-alpha", ",", "IL-1beta", ",", "TNF-alpha", "/", "IL-1beta", ",", "IL-6", ",", "platelet", "derived", "growth", "factor", ",", "lipopolysaccharide", ",", "or", "tetradecanoyl", "phorbol", "acetate", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["The", "slowly", "migrating", "complex", "could", "not", "be", "supershifted", "with", "antibodies", "against", "NF-kappaB", ",", "Jun", ",", "or", "the", "transcriptional", "coactivators", "p300", "or", "CBP", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "NF-kappaB-binding", "complex", "with", "similar", "slow", "mobility", "was", "observed", "in", "nuclear", "extracts", "prepared", "from", "fresh", "human", "RA", "synovial", "tissue", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 0], "tokens": ["CONCLUSION", ":", "Biological", "activity", "of", "TNF-alpha", "in", "SF", "from", "RA", "and", "OA", "subjects", "is", "capable", "of", "inducing", "p65/p50", "NF-kappaB", "DNA", "binding", "activity", "in", "macrophages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["A", "property", "of", "SF", "that", "is", "independent", "of", "TNF-alpha", "and", "other", "cytokines", "is", "responsible", "for", "the", "induction", "of", "a", "novel", "slowly", "migrating", "NF-kappaB-binding", "complex", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Soluble", "mediators", "in", "SF", "of", "subjects", "with", "RA", "and", "OA", "can", "therefore", "modulate", "binding", "of", "nuclear", "proteins", "to", "the", "NF-kappaB", "binding", "site", "in", "macrophages", "and", "may", "play", "a", "role", "in", "inflammatory", "gene", "expression", "in", "arthritis", "."]} {"tags": [3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Peroxisome", "proliferator", "activator", "receptor-gamma", "agonists", "and", "15-deoxy-Delta", "(", "12", ",", "14", ")", "(", "12", ",", "14", ")", "-PGJ", "(", "2", ")", "induce", "apoptosis", "in", "normal", "and", "malignant", "B-lineage", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 5, 6, 0], "tokens": ["The", "research", "described", "herein", "evaluates", "the", "expression", "and", "functional", "significance", "of", "peroxisome", "proliferator", "activator", "receptor-gamma", "(", "PPAR-gamma", ")", "on", "B-lineage", "cells", "."]} {"tags": [5, 6, 6, 6, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Normal", "mouse", "B", "cells", "and", "a", "variety", "of", "B", "lymphoma", "cells", "reflective", "of", "stages", "of", "B", "cell", "differentiation", "(", "e.g.", ",", "70Z/3", ",", "CH31", ",", "WEHI-231", ",", "CH12", ",", "and", "J558", ")", "express", "PPAR-gamma", "mRNA", "and", ",", "by", "Western", "blot", "analysis", ",", "the", "67-kDa", "PPAR-gamma", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["15-Deoxy-Delta", "(", "12", ",", "14", ")", "-PGJ", "(", "2", ")", "(", "15d-PGJ", "(", "2", ")", ")", ",", "a", "PPAR-gamma", "agonist", ",", "has", "a", "dose-dependent", "antiproliferative", "and", "cytotoxic", "effect", "on", "normal", "and", "malignant", "B", "cells", "as", "shown", "by", "[", "(", "3", ")", "H", "]", "thymidine", "and", "3-", "[", "4", ",", "5-dimethylthiazol-2-yl", "]", "-2", ",", "5-diphenyltetrazolium", "bromide", "assays", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 3, 0], "tokens": ["Only", "PPAR-gamma", "agonists", "(", "thiazolidinediones", ")", ",", "and", "not", "PPAR-alpha", "agonists", ",", "mimicked", "the", "effect", "of", "15d-PGJ", "(", "2", ")", "on", "B-lineage", "cells", ",", "indicating", "that", "the", "mechanism", "by", "which", "15d-PGJ", "(", "2", ")", "negatively", "affects", "B-lineage", "cells", "involves", "in", "part", "PPAR-gamma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "mechanism", "by", "which", "PPAR-gamma", "agonists", "induce", "cytotoxicity", "is", "via", "apoptosis", ",", "as", "shown", "by", "annexin", "V", "staining", "and", "as", "confirmed", "by", "DNA", "fragmentation", "detected", "using", "the", "TUNEL", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Interestingly", ",", "addition", "of", "PGF", "(", "2alpha", ")", ",", "which", "was", "not", "known", "to", "affect", "lymphocytes", ",", "dramatically", "attenuated", "the", "deleterious", "effects", "of", "PPAR-gamma", "agonists", "on", "B", "lymphomas", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Surprisingly", ",", "15d-PGJ", "(", "2", ")", "induced", "a", "massive", "increase", "in", "nuclear", "mitogen-activated", "protein", "kinase", "activation", ",", "and", "pretreatment", "with", "PGF", "(", "2alpha", ")", "blunted", "the", "mitogen-activated", "protein", "kinase", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["This", "is", "the", "first", "study", "evaluating", "PPAR-gamma", "expression", "and", "its", "significance", "on", "B", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["PPAR-gamma", "agonists", "may", "serve", "as", "a", "counterbalance", "to", "the", "stimulating", "effects", "of", "other", "PGs", ",", "namely", "PGE", "(", "2", ")", ",", "which", "promotes", "B", "cell", "differentiation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6], "tokens": ["Finally", ",", "the", "use", "of", "PGs", ",", "such", "as", "15d-PGJ", "(", "2", ")", ",", "and", "synthetic", "PPAR-gamma", "agonists", "to", "induce", "apoptosis", "in", "B-lineage", "cells", "may", "lead", "to", "the", "development", "of", "novel", "therapies", "for", "fatal", "B", "lymphomas"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "STAT3", "signaling", "leads", "to", "apoptosis", "of", "leukemic", "large", "granular", "lymphocytes", "and", "decreased", "Mcl-1", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Large", "granular", "lymphocyte", "(", "LGL", ")", "leukemia", "is", "characterized", "by", "the", "expansion", "of", "antigen-activated", "cytotoxic", "T", "lymphocytes", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["These", "leukemic", "cells", "are", "resistant", "to", "Fas-mediated", "apoptosis", "despite", "expressing", "high", "levels", "of", "Fas", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["We", "found", "that", "leukemic", "LGL", "from", "19", "patients", "displayed", "high", "levels", "of", "activated", "STAT3", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Treatment", "of", "leukemic", "LGL", "with", "the", "JAK-selective", "tyrosine", "kinase", "inhibitor", "AG-490", "induced", "apoptosis", "with", "a", "corresponding", "decrease", "in", "STAT", "-DNA", "binding", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Moreover", ",", "using", "an", "antisense", "oligonucleotide", "approach", "to", "diminish", "STAT3", "expression", ",", "we", "found", "that", "Fas", "sensitivity", "was", "restored", "in", "leukemic", "LGL", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["AG-490-induced", "apoptosis", "in", "leukemic", "LGL", "was", "independent", "of", "Bcl-xL", "or", "Bcl-2", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "we", "found", "that", "the", "Bcl-2-family", "protein", "Mcl-1", "was", "significantly", "reduced", "by", "AG-490", "treatment", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 0], "tokens": ["Activated", "STAT3", "was", "shown", "to", "bind", "an", "SIE-related", "element", "in", "the", "murine", "mcl-1", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "a", "luciferase", "reporter", "assay", ",", "we", "demonstrated", "that", "v-src", "overexpression", "in", "NIH3T3", "induced", "STAT3", "-dependent", "transcriptional", "activity", "from", "the", "mcl-1", "promoter", "and", "increased", "endogenous", "Mcl-1", "protein", "levels", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["We", "conclude", "that", "STAT3", "activation", "contributed", "to", "accumulation", "of", "the", "leukemic", "LGL", "clones", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "findings", "suggest", "that", "investigation", "should", "focus", "on", "novel", "strategies", "targeting", "STAT3", "in", "the", "treatment", "of", "LGL", "leukemia", "."]} {"tags": [0, 0, 1, 2, 0, 0, 3, 4, 4, 0, 0, 0], "tokens": ["Decreased", "immediate", "inflammatory", "gene", "induction", "in", "activating", "transcription", "factor-2", "mutant", "mice", "."]} {"tags": [3, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transcription", "factor", "activating", "transcription", "factor", "(", "ATF", ")", "-2", "is", "activated", "by", "inflammatory", "signals", "transduced", "by", "the", "JNK", "and", "p38", "MAP", "kinase", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["To", "better", "define", "the", "role", "of", "ATF-2", "in", "inflammation", ",", "adult", "mice", "expressing", "small", "amounts", "of", "a", "mutant", "ATF-2", "protein", "were", "challenged", "with", "lipopolysaccharide", "(", "LPS", ")", ",", "anti-CD3", "antibody", "or", "virus", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 0, 3, 0, 3, 0, 0, 0, 0, 3, 3, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Within", "3", "h", "of", "challenge", "by", "LPS", ",", "ATF-2", "mutant", "mice", "had", "decreased", "induction", "of", "the", "adhesion", "molecules", "E-selectin", ",", "P-selectin", "and", "VCAM-1", "as", "well", "as", "the", "cytokines", "tumor", "necrosis", "factor-alpha", ",", "IL-1beta", "and", "IL-6", "compared", "with", "control", "mice", "."]} {"tags": [0, 0, 5, 6, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Stimulation", "of", "T", "lymphocytes", "by", "anti-CD3", "antibody", "also", "showed", "less", "induction", "of", "IL-1", "and", "IL-6", "in", "ATF-2", "mutant", "tissues", "."]} {"tags": [5, 6, 6, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 3, 0], "tokens": ["ATF-2", "mutant", "thymocytes", "treated", "with", "anti-CD3", "antibody", "in", "vitro", "demonstrated", "reduced", "induction", "of", "c-Jun", ",", "JunB", ",", "JunD", "and", "Fra-2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "similar", "to", "what", "was", "observed", "after", "p38", "kinase", "inhibition", "in", "normal", "mice", ",", "relative", "ATF-2", "deficiency", "did", "not", "prevent", "the", "development", "of", "a", "mononuclear", "cell", "infiltrate", "in", "the", "week", "following", "an", "inflammatory", "stimulus", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["ATF-2", "mutant", "mice", "proved", "more", "susceptible", "to", "death", "than", "control", "mice", "from", "LPS", "plus", "D-galactosamine", "injection", "or", "Coxsackievirus", "B3", "infection", "and", "had", "a", "higher", "incidence", "of", "mononuclear", "pulmonary", "infiltrates", "after", "exposure", "to", "Herpes", "simplex", "virus-1", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["ATF-2", "is", "essential", "for", "maximal", "immediate", "induction", "of", "adhesion", "molecules", "and", "cytokine", "genes", ",", "but", "at", "later", "time", "points", "may", "even", "protect", "against", "overactive", "immune", "responses", "."]} {"tags": [3, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["LIGHT", ",", "a", "TNF-like", "molecule", ",", "costimulates", "T", "cell", "proliferation", "and", "is", "required", "for", "dendritic", "cell", "-mediated", "allogeneic", "T", "cell", "response", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 0, 0, 0, 0, 5, 6, 0, 5, 6, 0], "tokens": ["LIGHT", "is", "a", "recently", "identified", "member", "of", "the", "TNF", "superfamily", "and", "its", "receptors", ",", "herpesvirus", "entry", "mediator", "and", "lymphotoxin", "beta", "receptor", ",", "are", "found", "in", "T", "cells", "and", "stromal", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 6, 6, 0, 5, 0, 0, 0, 5, 6, 0], "tokens": ["In", "this", "study", ",", "we", "demonstrate", "that", "LIGHT", "is", "selectively", "expressed", "on", "immature", "dendritic", "cells", "(", "DCs", ")", "generated", "from", "human", "PBMCs", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 5, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "LIGHT", "is", "not", "detectable", "in", "DCs", "either", "freshly", "isolated", "from", "PBMCs", "or", "rendered", "mature", "in", "vitro", "by", "LPS", "treatment", "."]} {"tags": [0, 0, 3, 0, 0, 3, 4, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Blockade", "of", "LIGHT", "by", "its", "soluble", "receptors", ",", "lymphotoxin", "beta", "receptor-Ig", "or", "HVEM-Ig", ",", "inhibits", "the", "induction", "of", "DC", "-mediated", "primary", "allogeneic", "T", "cell", "response", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "engagement", "of", "LIGHT", "costimulates", "human", "T", "cell", "proliferation", ",", "amplifies", "the", "NF-kappaB", "signaling", "pathway", ",", "and", "preferentially", "induces", "the", "production", "of", "IFN-gamma", ",", "but", "not", "IL-4", ",", "in", "the", "presence", "of", "an", "antigenic", "signal", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 3, 4, 0, 0, 5, 0, 0, 0, 0, 0], "tokens": ["Our", "results", "suggest", "that", "LIGHT", "is", "a", "costimulatory", "molecule", "involved", "in", "DC", "-mediated", "cellular", "immune", "responses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Suppression", "of", "HIV", "type", "1", "replication", "by", "a", "dominant-negative", "Ets-1", "mutant", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 1, 2, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Activity", "of", "the", "distal", "region", "of", "the", "human", "immunodeficiency", "virus", "(", "HIV-1", ")", "long", "terminal", "repeat", "(", "LTR", ")", ",", "which", "contains", "binding", "sites", "for", "the", "Ets-1", "and", "USF-1", "proteins", ",", "is", "integral", "for", "HIV-1", "replication", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 1, 0], "tokens": ["The", "Ets-1", "and", "USF-1", "proteins", "play", "a", "critical", "role", "in", "the", "activity", "of", "the", "HIV-1", "LTR", "distal", "enhancer", "region", ",", "as", "indicated", "by", "the", "potent", "dominant", "negative", "effect", "of", "a", "mutant", "Ets-1", "lacking", "trans-activation", "domains", "on", "the", "transcriptional", "activity", "of", "the", "LTR", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["To", "determine", "the", "biological", "relevance", "of", "the", "Ets-1", "and", "USF-1", "proteins", "in", "HIV-1", "replication", ",", "we", "examined", "the", "effect", "of", "expression", "of", "the", "dominant-negative", "mutant", "of", "Ets-1", "(", "dnEts-1", ")", "on", "HIV-1", "infection", "of", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["We", "demonstrated", "that", "expression", "of", "dnEts", "markedly", "suppressed", "HIV-1", "infection", "of", "a", "T", "cell", "line", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "finding", "indicates", "that", "formation", "of", "a", "transcriptionaly", "active", "USF-1/Ets-1", "complex", "is", "important", "in", "the", "productive", "infection", "of", "cells", "by", "HIV-1", ",", "and", "suggests", "that", "inhibition", "of", "the", "interaction", "between", "USF-1", "and", "Ets-1", "with", "the", "HIV-1", "LTR", "may", "provide", "a", "new", "target", "for", "anti-HIV-1", "gene", "therapy", "."]} {"tags": [5, 6, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 0], "tokens": ["Human", "eosinophils", "constitutively", "express", "nuclear", "factor", "of", "activated", "T", "cells", "p", "and", "c", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BACKGROUND", ":", "Eosinophils", "are", "now", "known", "to", "produce", "a", "variety", "of", "proinflammatory", "cytokines", ",", "although", "the", "molecular", "factors", "that", "regulate", "their", "production", "are", "poorly", "understood", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["The", "expression", "of", "almost", "all", "of", "the", "cytokines", "produced", "by", "eosinophils", ",", "including", "the", "proallergic", "cytokine", "IL-4", ",", "is", "now", "known", "to", "be", "regulated", "at", "the", "level", "of", "transcription", "by", "members", "of", "the", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NFAT", ")", "family", "of", "transcription", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 0], "tokens": ["OBJECTIVE", ":", "We", "sought", "to", "characterize", "the", "expression", "of", "different", "NFAT", "proteins", "in", "resting", "and", "activated", "eosinophils", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Nuclear", "and", "whole", "cell", "extracts", "were", "obtained", "from", "both", "peripheral", "blood", "eosinophils", "and", "those", "obtained", "from", "bronchoalveolar", "lavage", "fluid", "of", "asthmatic", "subjects", "after", "endobronchial", "allergen", "challenge", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0], "tokens": ["NFAT", "expression", "was", "determined", "by", "using", "immunoprecipitation", "and", "Western", "blot", "analysis", ",", "DNA-binding", "assays", ",", "and", "RT-PCR", "analysis", "of", "eosinophil", "mRNA", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0, 3, 4, 4, 4, 0], "tokens": ["RESULTS", ":", "Both", "peripheral", "blood", "and", "bronchoalveolar", "lavage", "fluid", "eosinophils", "expressed", "NFATp", "and", "NFATc", "protein", "."]} {"tags": [0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Unlike", "activated", "T", "cells", ",", "which", "express", "multiple", "NFATc", "isoforms", ",", "eosinophils", "preferentially", "express", "the", "approximately", "85-kd", "isoform", "."]} {"tags": [0, 0, 0, 5, 0, 0, 0, 0, 0, 9, 10, 0], "tokens": ["In", "addition", ",", "eosinophils", "were", "found", "to", "constitutively", "express", "NFATc", "mRNA", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["A", "brief", "incubation", "with", "the", "T", "(", "H", ")", "2", "cytokines", "IL-4", "and", "IL-5", "was", "sufficient", "to", "induce", "the", "nuclear", "translocation", "of", "NFATc", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Eosinophil", "nuclear", "extracts", "contain", "multiple", "factors", "that", "can", "specifically", "recognize", "the", "IL-4", "promoter", "P1", "NFAT", "site", "in", "DNA-binding", "assays", ",", "including", "NFATp", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSION", ":", "NFATp", "and", "NFATc", "can", "regulate", "the", "expression", "of", "cytokines", "and", "other", "genes", "in", "eosinophils", "but", "appear", "to", "be", "regulated", "by", "a", "novel", "signal", "transduction", "mechanism", "in", "these", "cells", "."]} {"tags": [3, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 3, 0, 3, 0], "tokens": ["BLyS", "BINDS", "TO", "B", "CELLS", "WITH", "HIGH", "AFFINITY", "AND", "INDUCES", "ACTIVATION", "OF", "THE", "TRANSCRIPTION", "FACTORS", "NF-kappaB", "AND", "ELF-1", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["B", "lymphocyte", "stimulator", "(", "BLyS", ")", "is", "a", "novel", "member", "of", "the", "TNF", "family", "of", "proteins", "expressed", "by", "myeloid", "cells", "as", "membrane-bound", "and", "soluble", "forms", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BLyS", "was", "shown", "to", "act", "specifically", "on", "B", "cells", ",", "inducing", "proliferation", "and", "immunoglobulin", "production", "both", "in", "vitro", "and", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["The", "present", "study", "was", "undertaken", "to", "characterize", "binding", "of", "radiolabeled", "BLyS", "to", "its", "cognate", "receptor", "on", "human", "B", "lymphocytes", "and", "examine", "intracellular", "events", "initiated", "by", "BLyS", "binding", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Similar", "to", "other", "TNF", "family", "members", ",", "BLyS", "is", "present", "in", "solution", "as", "a", "homotrimer", "as", "determined", "by", "gel", "filtration", "chromatography", "and", "light", "scattering", "analysis", "."]} {"tags": [3, 0, 0, 5, 6, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["BLyS", "binding", "to", "B", "cells", "is", "specific", "as", "other", "TNF", "family", "members", "tested", "did", "not", "compete", "for", "(", "125", ")", "I-", "BLyS", "binding", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0], "tokens": ["Analysis", "of", "equilibrium", "binding", "of", "(", "125", ")", "I-labeled", "BLyS", "to", "purified", "human", "tonsillar", "B", "cells", "demonstrated", "saturable", "binding", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Scatchard", "analysis", "of", "the", "binding", "data", "revealed", "a", "single", "class", "of", "high-affinity", "binding", "on", "human", "B", "cells", "with", "approximately", "2600", "binding", "sites", "per", "cell", "and", "an", "apparent", "dissociation", "constant", "(", "K", "(", "D", ")", ")", "of", "about", "0.1", "nM", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0], "tokens": ["In", "addition", "we", "report", "that", "BLyS", "binding", "to", "B", "cells", "results", "in", "the", "activation", "of", "NF-kappaB", "and", "the", "Ets", "family", "transcription", "factor", ",", "ELF-1", ",", "and", "in", "the", "induction", "of", "mRNA", "for", "Polo-like", "kinase", "(", "PLK", ")", "."]} {"tags": [0, 0, 0, 0, 0], "tokens": ["Copyright", "2001", "Academic", "Press", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Design", "and", "use", "of", "an", "inducibly", "activated", "human", "immunodeficiency", "virus", "type", "1", "Nef", "to", "study", "immune", "modulation", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "Nef", "protein", "of", "the", "human", "immunodeficiency", "virus", "type", "1", "(", "HIV-1", ")", "has", "been", "shown", "to", "enhance", "the", "infectivity", "of", "virus", "particles", ",", "downmodulate", "cell", "surface", "proteins", ",", "and", "associate", "with", "many", "intracellular", "proteins", "that", "are", "thought", "to", "facilitate", "HIV", "infection", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0], "tokens": ["One", "of", "the", "challenges", "in", "defining", "the", "molecular", "events", "regulated", "by", "Nef", "has", "been", "obtaining", "good", "expression", "of", "Nef", "protein", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["This", "has", "been", "attributed", "to", "effects", "of", "Nef", "on", "cell", "proliferation", "and", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "designed", "a", "Nef", "protein", "that", "is", "readily", "expressed", "in", "T-cell", "lines", "and", "whose", "function", "is", "inducibly", "activated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0], "tokens": ["It", "is", "composed", "of", "a", "fusion", "between", "full-length", "Nef", "and", "the", "estrogen", "receptor", "hormone-binding", "domain", "(", "Nef-ER", ")", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["The", "Nef-ER", "is", "kept", "in", "an", "inactive", "state", "due", "to", "steric", "hindrance", ",", "and", "addition", "of", "the", "membrane-permeable", "drug", "4-hydroxytamoxifen", "(", "4-HT", ")", ",", "which", "binds", "to", "the", "ER", "domain", ",", "leads", "to", "inducible", "activation", "of", "Nef-ER", "within", "cells", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "demonstrate", "that", "Nef-ER", "inducibly", "associates", "with", "the", "62-kDa", "Ser/Thr", "kinase", "and", "is", "localized", "to", "specific", "membrane", "microdomains", "(", "lipid", "rafts", ")", "only", "after", "activation", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Using", "this", "inducible", "Nef", ",", "we", "also", "compared", "the", "specific", "requirements", "for", "CD4", "and", "HLA-A2", "downmodulation", "in", "a", "SupT1", "T-cell", "line", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Half-maximal", "downmodulation", "of", "cell", "surface", "CD4", "required", "very", "little", "active", "Nef-ER", "and", "occurred", "as", "early", "as", "4", "h", "after", "addition", "of", "4-HT", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "50", "%", "downmodulation", "of", "HLA-A2", "by", "Nef", "required", "16", "to", "24", "h", "and", "about", "50-", "to", "100-fold-greater", "concentrations", "of", "4-HT", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["These", "data", "suggest", "that", "HLA-A2", "downmodulation", "may", "require", "certain", "threshold", "levels", "of", "active", "Nef", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "differential", "timing", "of", "CD4", "and", "HLA-A2", "downmodulation", "may", "have", "implications", "for", "HIV", "pathogenesis", "and", "immune", "evasion", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Regulation", "of", "chemokine", "mRNA", "expression", "in", "a", "rat", "model", "of", "vanadium-induced", "pulmonary", "inflammation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Environmental", "and", "occupational", "exposure", "to", "vanadium", "dusts", "results", "in", "toxic", "effects", "mainly", "confined", "to", "the", "respiratory", "system", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0], "tokens": ["Using", "a", "rat", "model", "of", "acute", "lung", "inflammation", "induced", "by", "intratracheal", "instillation", "of", "sodium", "metavanadate", "(", "NaVO3", ")", "at", "the", "dose", "of", "200", "microg", "V/kg", ",", "we", "investigated", "the", "relationship", "between", "the", "cytologic", "characterization", "of", "pulmonary", "inflammation", "and", "the", "expression", "of", "chemokine", "mRNA", "."]} {"tags": [0, 5, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["Significant", "polymorphonuclear", "leukocyte", "(", "PMN", ")", "influx", "(", "P", "<", "0.01", ")", "into", "the", "lung", "was", "noted", "4", "h", "after", "NaVO3", "instillation", ",", "whereas", "alveolar", "macrophages", "(", "AMs", ")", "in", "bronchoalveolar", "lavage", "(", "BAL", ")", "cells", "appeared", "to", "decrease", "significantly", "."]} {"tags": [0, 0, 0, 0, 5, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "neither", "PMNs", "nor", "AMs", "changed", "substantially", "1", "h", "after", "NaVO3", "instillation", "."]} {"tags": [0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["By", "Northern", "analysis", ",", "macrophage", "inflammatory", "protein", "(", "MIP", ")", "-2", "mRNA", "in", "BAL", "cells", "increased", "markedly", "1", "h", "after", "NaVO3", "instillation", "and", "reduced", "a", "little", "bit", "at", "4", "h", ",", "whereas", "MIP-1alpha", "mRNA", "in", "BAL", "cells", "was", "expressed", "relatively", "high", "1", "h", "after", "NaVO3", "instillation", ",", "although", "a", "basal", "expression", "was", "detected", "in", "control", "group", ",", "and", "returned", "rapidly", "nearly", "to", "control", "level", "at", "4", "h", "."]} {"tags": [0, 3, 0, 0, 0, 5, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Since", "MIP-2", "is", "a", "potent", "PMN", "chemoattractant", "and", "MIP-1alpha", "is", "a", "potent", "macrophage/monocyte", "chemoattractant", "has", "been", "well", "known", "."]} {"tags": [0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 5, 0, 5, 6, 0, 0, 5, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "facts", "that", "PMN", "influx", "was", "preceded", "by", "increased", "MIP-2", "mRNA", "expression", ",", "suggesting", "that", "MIP-2", "is", "involved", "in", "the", "development", "of", "NaVO3-induced", "pulmonary", "inflammation", ",", "whereas", "increased", "MIP-1alpha", "mRNA", "expression", "was", "followed", "by", "decreased", "AMs", "in", "BAL", "cells", ",", "suggesting", "AMs", "might", "be", "activated", "by", "MIP-1alpha", ",", "adherent", "to", "the", "lining", "surface", "of", "the", "airways", "and", "then", "resistant", "to", "be", "washed", "out", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0], "tokens": ["To", "delineate", "the", "mechanisms", "of", "transcriptional", "activation", ",", "we", "recently", "cloned", "the", "5'-flanking", "region", "of", "the", "MIP-2", "gene", "."]} {"tags": [0, 1, 2, 0, 1, 2, 2, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 3, 4, 0, 3, 0, 0], "tokens": ["The", "promotor", "region", "contains", "consensus", "binding", "sites", "for", "transcription", "factor", "nuclear", "factor", "kappaB", "(", "NF-kappaB", ")", "and", "activator", "protein-1", "(", "AP-1", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0], "tokens": ["Using", "electrophoretic", "mobility", "shift", "assay", ",", "increased", "nuclear", "NF-kappaB", ",", "not", "AP-1", ",", "binding", "activity", "was", "detected", "1", "h", "after", "NaVO3", "instillation", ",", "which", "correlated", "with", "the", "induction", "of", "MIP-2", "mRNA", "."]} {"tags": [3, 4, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["p65", "(", "Rel", "A", ")", "and", "p50", "protein", "appears", "to", "be", "involved", "in", "MIP-2", "NF-kappaB", "binding", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Taken", "together", ",", "our", "studies", "suggest", "that", "MIP-2", "is", "an", "important", "mediator", "of", "NaVO3-induced", "pulmonary", "inflammation", "in", "the", "rat", "model", "."]} {"tags": [0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 3, 0, 0, 0, 3, 0], "tokens": ["In", "addition", ",", "elevated", "MIP-2", "mRNA", "levels", "are", "accompanied", "by", "increased", "NF-kappaB", "binding", "activity", "in", "BAL", "cells", ",", "suggesting", "possible", "MIP-2", "transcriptional", "regulation", "through", "NF-kappaB", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 5, 6, 0], "tokens": ["Activation", "of", "signal", "transduction", "and", "apoptosis", "in", "healthy", "lymphomonocytes", "exposed", "to", "bystander", "HIV-1-infected", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Persistent", "activation", "of", "the", "immune", "system", "is", "one", "of", "the", "hallmarks", "of", "HIV-1", "infection", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 9, 10, 0, 0, 5, 6, 6, 6, 6, 0, 5, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", "we", "analysed", "the", "induction", "of", "factors", "involved", "in", "cytokine", "signal", "transduction", ",", "such", "as", "STAT", "1", "proteins", "and", "IRF-1", "mRNA", ",", "in", "normal", "peripheral", "blood", "mononuclear", "cells", "(", "PBMC", ")", "exposed", "to", "HIV-infected", "cells", ",", "and", "the", "induction", "of", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 9, 10, 0], "tokens": ["Western", "blot", "analyses", "and", "reverse", "transcriptase-polymerase", "chain", "reaction", "results", "indicate", "that", "both", "cells", "infected", "with", "a", "X4", "strain", "and", "cells", "infected", "with", "a", "R5", "strain", "are", "able", "to", "increase", "intracellular", "levels", "of", "STAT", "1alpha", "and", "beta", "proteins", "as", "well", "as", "IRF-1", "mRNA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0], "tokens": ["This", "effect", "was", "prevented", "by", "neutralizing", "antibodies", "against", "interferon-alpha", "(", "IFN-alpha", ")", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0], "tokens": ["HIV-1-infected", "cells", "dose-dependently", "induced", "apoptotic", "commitment", "in", "normal", "PBMC", ",", "as", "revealed", "by", "DNA", "fragmentation", "analysis", ",", "but", "this", "was", "not", "accompanied", "by", "an", "increase", "of", "caspase-3", "activity", ",", "even", "if", "a", "slight", "up-regulation", "of", "IL-1beta-converting", "enzyme", "mRNA", "was", "detected", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Apoptosis", "induction", "could", "be", "abrogated", "mainly", "by", "antibodies", "against", "tumour", "necrosis", "factor-alpha", "(", "TNF-alpha", ")", "and", ",", "to", "a", "lesser", "extent", ",", "by", "antibodies", "against", "IFN-gamma", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 3, 0], "tokens": ["All", "these", "findings", "suggest", "that", "uninfected", "PBMC", "can", "undergo", "activation", "of", "signal", "transduction", "and", "apoptosis", "after", "exposure", "to", "bystander", "HIV-infected", "cells", ",", "subsequent", "to", "the", "induction", "of", "cytokines", "such", "as", "IFNs", "and", "TNF-alpha", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["The", "physical", "association", "of", "protein", "kinase", "C", "theta", "with", "a", "lipid", "raft-associated", "inhibitor", "of", "kappa", "B", "factor", "kinase", "(", "IKK", ")", "complex", "plays", "a", "role", "in", "the", "activation", "of", "the", "NF-kappa", "B", "cascade", "by", "TCR", "and", "CD28", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0], "tokens": ["We", "investigated", "the", "role", "of", "protein", "kinase", "C", "theta", "(", "PKCtheta", ")", "in", "the", "activation", "of", "the", "NF-kappaB", "cascade", "in", "primary", "human", "CD4", "(", "+", ")", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Among", "six", "or", "so", "PKC", "isoforms", "expressed", "in", "T", "cells", ",", "only", "PKCtheta", "participates", "in", "the", "assembly", "of", "the", "supramolecular", "activation", "clusters", "at", "the", "contact", "site", "of", "the", "TCR", "with", "Ag", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Signaling", "via", "both", "the", "TCR", "and", "CD28", "is", "required", "for", "optimal", "activation", "of", "the", "multisubunit", "IkappaB", "kinase", "(", "IKK", ")", "complex", "in", "primary", "human", "T", "lymphocytes", ";", "this", "activation", "could", "be", "inhibited", "by", "a", "Ca", "(", "2+", ")", "-independent", "PKC", "isoform", "inhibitor", ",", "rottlerin", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 6, 6, 6, 6, 0], "tokens": ["Moreover", ",", "endogenous", "PKCtheta", "physically", "associates", "with", "activated", "IKK", "complexes", "in", "CD3/CD28-costimulated", "primary", "CD4", "(", "+", ")", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["The", "same", "set", "of", "stimuli", "also", "induced", "relocation", "of", "endogenous", "PKCtheta", "and", "IKKs", "to", "a", "GM1", "ganglioside-enriched", ",", "detergent-insoluble", "membrane", "compartment", "in", "primary", "T", "cells", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["IKKs", "recruited", "to", "these", "lipid", "rafts", "were", "capable", "of", "phosphorylating", "a", "recombinant", "IkappaBalpha", "sustrate", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0], "tokens": ["Confocal", "microscopy", "further", "demonstrated", "that", "exogenously", "expressed", "PKCtheta", "and", "IKKss", "colocalize", "in", "the", "membrane", "of", "CD3/CD28-costimulated", "Jurkat", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 3, 0, 7, 8, 8, 0], "tokens": ["Constitutively", "active", "but", "not", "kinase-inactive", "PKCtheta", "activated", "IKKbeta", "in", "Jurkat", "T", "cells", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 0], "tokens": ["Expression", "of", "dominant-active", "PKCtheta", "also", "had", "stimulatory", "effects", "on", "the", "CD28", "response", "element", "of", "the", "IL-2", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Taken", "together", ",", "these", "data", "show", "that", "the", "activation", "of", "PKCtheta", "by", "the", "TCR", "and", "CD28", "plays", "an", "important", "role", "in", "the", "assembly", "and", "activation", "of", "IKK", "complexes", "in", "the", "T", "cell", "membrane"]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["T-cell", "-mediated", "regulation", "of", "osteoclastogenesis", "by", "signalling", "cross-talk", "between", "RANKL", "and", "IFN-gamma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 3, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Bone", "resorption", "is", "regulated", "by", "the", "immune", "system", ",", "where", "T-cell", "expression", "of", "RANKL", "(", "receptor", "activator", "of", "nuclear", "factor", "(", "NF", ")", "-kappaB", "ligand", ")", ",", "a", "member", "of", "the", "tumour-necrosis", "factor", "family", "that", "is", "essential", "for", "osteoclastogenesis", ",", "may", "contribute", "to", "pathological", "conditions", ",", "such", "as", "autoimmune", "arthritis", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["However", ",", "whether", "activated", "T", "cells", "maintain", "bone", "homeostasis", "by", "counterbalancing", "the", "action", "of", "RANKL", "remains", "unknown", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Here", "we", "show", "that", "T-cell", "production", "of", "interferon", "(", "IFN", ")", "-gamma", "strongly", "suppresses", "osteoclastogenesis", "by", "interfering", "with", "the", "RANKL", "-RANK", "signalling", "pathway", "."]} {"tags": [3, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 3, 0, 3, 0], "tokens": ["IFN-gamma", "induces", "rapid", "degradation", "of", "the", "RANK", "adapter", "protein", ",", "TRAF6", "(", "tumour", "necrosis", "factor", "receptor-associated", "factor", "6", ")", ",", "which", "results", "in", "strong", "inhibition", "of", "the", "RANKL", "-induced", "activation", "of", "the", "transcription", "factor", "NF-kappaB", "and", "JNK", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["This", "inhibition", "of", "osteoclastogenesis", "is", "rescued", "by", "overexpressing", "TRAF6", "in", "precursor", "cells", ",", "which", "indicates", "that", "TRAF6", "is", "the", "target", "critical", "for", "the", "IFN-gamma", "action", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "we", "provide", "evidence", "that", "the", "accelerated", "degradation", "of", "TRAF6", "requires", "both", "its", "ubiquitination", ",", "which", "is", "initiated", "by", "RANKL", ",", "and", "IFN-gamma", "-induced", "activation", "of", "the", "ubiquitin-proteasome", "system", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0], "tokens": ["Our", "study", "shows", "that", "there", "is", "cross-talk", "between", "the", "tumour", "necrosis", "factor", "and", "IFN", "families", "of", "cytokines", ",", "through", "which", "IFN-gamma", "provides", "a", "negative", "link", "between", "T-cell", "activation", "and", "bone", "resorption", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "results", "may", "offer", "a", "therapeutic", "approach", "to", "treat", "the", "inflammation-induced", "tissue", "breakdown", "."]} {"tags": [3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Stromal-derived", "factor", "1", "and", "thrombopoietin", "regulate", "distinct", "aspects", "of", "human", "megakaryopoiesis", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["The", "role", "of", "the", "chemokine", "binding", "stromal-derived", "factor", "1", "(", "SDF-1", ")", "in", "normal", "human", "megakaryopoiesis", "at", "the", "cellular", "and", "molecular", "levels", "and", "its", "comparison", "with", "that", "of", "thrombopoietin", "(", "TPO", ")", "have", "not", "been", "determined", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "study", "it", "was", "found", "that", "SDF-1", ",", "unlike", "TPO", ",", "does", "not", "stimulate", "alpha", "(", "IIb", ")", "beta", "(", "3", ")", "(", "+", ")", "cell", "proliferation", "or", "differentiation", "or", "have", "an", "antiapoptotic", "effect", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 3, 0, 3, 0], "tokens": ["However", ",", "it", "does", "induce", "chemotaxis", ",", "trans-Matrigel", "migration", ",", "and", "secretion", "of", "matrix", "metalloproteinase", "9", "(", "MMP-9", ")", "and", "vascular", "endothelial", "growth", "factor", "(", "VEGF", ")", "by", "these", "cells", ",", "and", "both", "SDF-1", "and", "TPO", "increase", "the", "adhesion", "of", "alpha", "(", "IIb", ")", "beta", "(", "3", ")", "(", "+", ")", "cells", "to", "fibrinogen", "and", "vitronectin", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 3, 4, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 3, 4, 0, 0], "tokens": ["Investigating", "the", "intracellular", "signaling", "pathways", "induced", "by", "SDF-1", "and", "TPO", "revealed", "some", "overlapping", "patterns", "of", "protein", "phosphorylation/activation", "(", "mitogen-activated", "protein", "kinase", "[", "MAPK", "]", "p42/44", ",", "MAPK", "p38", ",", "and", "AKT", "[", "protein", "kinase", "B", "]", ")", "and", "some", "that", "were", "distinct", "for", "TPO", "(", "eg", ",", "JAK-STAT", ")", "and", "for", "SDF-1", "(", "eg", ",", "NF-kappa", "B", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "was", "also", "found", "that", "though", "inhibition", "of", "phosphatidyl-inositol", "3-kinase", "(", "PI-3K", ")", "by", "LY294002", "in", "alpha", "(", "IIb", ")", "beta", "(", "3", ")", "(", "+", ")", "cells", "induced", "apoptosis", "and", "inhibited", "chemotaxis", "adhesion", "and", "the", "secretion", "of", "MMP-9", "and", "VEGF", ",", "the", "inhibition", "of", "MAPK", "p42/44", "(", "by", "the", "MEK", "inhibitor", "U0126", ")", "had", "no", "effect", "on", "the", "survival", ",", "proliferation", ",", "and", "migration", "of", "these", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Hence", ",", "it", "is", "suggested", "that", "the", "proliferative", "effect", "of", "TPO", "is", "more", "related", "to", "activation", "of", "the", "JAK-STAT", "pathway", "(", "unique", "to", "TPO", ")", ",", "and", "the", "PI-3K-AKT", "axis", "is", "differentially", "involved", "in", "TPO-", "and", "SDF-1-dependent", "signaling", "."]} {"tags": [0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0], "tokens": ["Accordingly", ",", "PI-3K", "is", "involved", "in", "TPO", "-mediated", "inhibition", "of", "apoptosis", ",", "TPO-", "and", "SDF-1-regulated", "adhesion", "to", "fibrinogen", "and", "vitronectin", ",", "and", "SDF-1", "-mediated", "migration", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "study", "expands", "the", "understanding", "of", "the", "role", "of", "SDF-1", "and", "TPO", "in", "normal", "human", "megakaryopoiesis", "and", "indicates", "the", "molecular", "basis", "of", "the", "observed", "differences", "in", "cellular", "responses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["(", "Blood.", "2000", ";", "96", ":", "4142-4151", ")"]} {"tags": [0, 0, 5, 6, 6, 6, 6, 0, 0, 5, 6, 6, 6, 0, 5, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Adhesion", "of", "immature", "and", "mature", "T", "cells", "induces", "in", "human", "thymic", "epithelial", "cells", "(", "TEC", ")", "activation", "of", "IL-6", "gene", "trascription", "factors", "(", "NF-kappaB", "and", "NF-IL6", ")", "and", "IL-6", "gene", "expression", ":", "role", "of", "alpha3beta1", "and", "alpha6beta4", "integrins", "."]} {"tags": [5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["T", "cell", "precursors", "homed", "to", "thymus", "develop", "in", "close", "contact", "with", "stromal", "cells", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Among", "them", ",", "thymic", "epithelial", "cells", "(", "TEC", ")", "are", "known", "to", "exert", "dominant", "roles", "in", "their", "survival", "and", "functional", "shaping", "."]} {"tags": [0, 0, 0, 5, 0, 5, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["Key", "molecules", "mediating", "TEC", "/", "thymocytes", "interactions", "include", "cytokines", "and", "growth", "factors", "secreted", "by", "the", "two", "cell", "types", "and", "adhesion", "receptors", "mediating", "cell", "contact", "."]} {"tags": [0, 0, 0, 0, 5, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Signaling", "events", "triggered", "in", "thymocytes", "by", "adhesion", "to", "epithelial", "cells", "have", "been", "extensively", "investigated", ",", "whereas", "little", "is", "known", "on", "the", "opposite", "phenomenon", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["We", "have", "previously", "investigated", "this", "issue", "in", "a", "co-culture", "system", "composed", "of", "TEC", "cultures", "derived", "from", "human", "normal", "thymus", "and", "heterologous", "thymocytes", "."]} {"tags": [0, 0, 0, 5, 0, 0, 5, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0], "tokens": ["We", "demonstrated", "that", "thymocytes", "adhere", "to", "TEC", "involving", "beta1", "and", "beta4", "integrins", "and", "induce", "the", "clustering", "of", "alpha3beta1", "and", "alpha6beta4", "heterodimers", "at", "the", "TEC", "surface", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 3, 4, 4, 0, 0, 0, 0, 0], "tokens": ["In", "addition", "thymocyte", "adhesion", "was", "followed", "by", "activation", "of", "NF-kappaB", "and", "NF-IL6", "gene", "transcription", "factors", "and", "enhanced", "IL-6", "production", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["The", "two", "latter", "phenomena", "were", "reproduced", "by", "the", "cross-linking", "of", "the", "alpha3", ",", "alpha6", ",", "beta1", "and", "beta4", "integrins", ",", "thus", "implying", "that", "the", "alpha3beta1", "and", "alpha6beta4", "heterodimers", "can", "signal", "during", "thymocyte", "adhesion", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["We", "have", "extended", "our", "previous", "work", "investigating", "in", "the", "same", "experimental", "setting", "the", "inducing", "activity", "of", "non", "stimulated", "or", "activated", "policlonal", "or", "clonal", "mature", "T", "cells", "as", "representative", "of", "the", "more", "mature", "thymocyte", "subset", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0], "tokens": ["We", "found", "that", "adhesion", "of", "unstimulated", "T", "cell", "i", ")", "involved", "beta1", ",", "but", "not", "beta4", "integrin", "functions", "at", "the", "surface", "ii", ")", "induced", "the", "clustering", "of", "alpha3beta1", ",", "but", "not", "alpha2beta1", "heterodimers", "at", "the", "TEC", "surface", "and", "iii", ")", "up-regulated", "the", "nuclear", "binding", "activity", "of", "NF-kappaB", "transcription", "factor", "and", "the", "IL-6", "secretion", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "propose", "that", "alpha3beta1", "and", "alpha6beta4", "heterodimers", "are", "induced", "to", "cluster", "at", "the", "TEC", "surface", "recognizing", "yet", "unknown", "cellular", "ligands", "differentially", "expressed", "during", "T", "cell", "development", "."]} {"tags": [0, 0, 0, 0, 1, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["Identification", "and", "characterization", "of", "SKAT-2", ",", "a", "novel", "Th2-specific", "zinc", "finger", "gene", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["We", "have", "identified", "a", "novel", "Kruppel-type", "zinc", "finger", "(", "ZF", ")", "gene", ",", "SKAT-2", ",", "which", "is", "selectively", "expressed", "by", "murine", "Th2", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 1, 2, 0, 1, 2, 0], "tokens": ["The", "protein", "encoded", "by", "this", "gene", "has", "14", "C2H2-type", "ZF", "tandemly", "arrayed", "at", "its", "C", "terminus", "and", "N-terminal", "SCAN", "box", "and", "KRAB", "domains", "."]} {"tags": [1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["SKAT-2", "is", "tissue", "restricted", "in", "expression", "at", "the", "RNA", "level", ",", "detectable", "only", "in", "brain", "and", "at", "low", "levels", "in", "kidney", "and", "spleen", "and", "few", "hematopoietic", "cell", "lines", "."]} {"tags": [0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["By", "in", "situ", "hybridization", ",", "SKAT-2", "expression", "was", "found", "to", "peak", "in", "antigen-stimulated", "CD4", "(", "+", ")", "T", "cells", "after", "2-3", "days", "of", "culture", "under", "Th2", "but", "not", "Th1", "biasing", "conditions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["This", "pattern", "of", "expression", "closely", "mirrored", "that", "of", "GATA-3", "in", "the", "same", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 1, 0], "tokens": ["In", "transient", "transfection", "experiments", "in", "phorbol", "12-myristate", "13-acetate/ionomycin-stimulated", "EL4", "cells", ",", "SKAT-2", "was", "found", "to", "up-regulate", "the", "activity", "of", "the", "IL-4", "but", "not", "the", "IL-5", "promoter", ",", "contrasting", "with", "the", "ability", "of", "GATA-3", "to", "activate", "both", "promoters", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 1, 0, 0, 1, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 0], "tokens": ["This", "result", "was", "confirmed", "using", "clones", "of", "EL4", "cells", "stably", "expressing", "an", "inducible", "form", "of", "SKAT-2", ",", "thus", "SKAT-2", "is", "a", "novel", "Th2-specific", "gene", "that", "may", "play", "a", "role", "in", "selective", "regulation", "of", "cytokine", "genes", "in", "T", "cells", "."]} {"tags": [3, 4, 4, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["hsp70", "interacting", "protein", "Hip", "does", "not", "affect", "glucocorticoid", "receptor", "folding", "by", "the", "hsp90-based", "chaperone", "machinery", "except", "to", "oppose", "the", "effect", "of", "BAG-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0], "tokens": ["Reticulocyte", "lysate", "contains", "a", "chaperone", "system", "that", "assembles", "glucocorticoid", "receptor", "(", "GR", ")", ".hsp90", "heterocomplexes", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "purified", "proteins", ",", "we", "have", "prepared", "a", "five-protein", "heterocomplex", "assembly", "system", "consisting", "of", "two", "proteins", "essential", "for", "heterocomplex", "assembly-", "hsp90", "and", "hsp70", "-and", "three", "proteins", "that", "act", "as", "co-chaperones", "to", "enhance", "assembly-", "Hop", ",", "hsp40", ",", "p23", "[", "Morishima", ",", "Y.", ",", "Kanelakis", ",", "K.", "C.", ",", "Silverstein", ",", "A.", "M.", ",", "Dittmar", ",", "K.", "D.", ",", "Estrada", ",", "L.", ",", "and", "Pratt", ",", "W.", "B.", "(", "2000", ")", "J.", "Biol.", "Chem.", "275", ",", "6894-6900", "]", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "hsp70", "co-chaperone", "Hip", "has", "been", "recovered", "in", "receptor.hsp90", "heterocomplexes", "at", "an", "intermediate", "stage", "of", "assembly", "in", "reticulocyte", "lysate", ",", "and", "Hip", "is", "also", "thought", "to", "be", "an", "intrinsic", "component", "of", "the", "assembly", "machinery", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "show", "that", "immunodepletion", "of", "Hip", "from", "reticulocyte", "lysate", "or", "addition", "of", "high", "levels", "of", "Hip", "to", "the", "purified", "five-protein", "system", "does", "not", "affect", "GR.hsp90", "heterocomplex", "assembly", "or", "the", "activation", "of", "steroid", "binding", "activity", "that", "occurs", "with", "assembly", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Despite", "the", "fact", "that", "Hip", "does", "not", "affect", "assembly", ",", "it", "is", "recovered", "in", "GR.hsp90", "heterocomplexes", "assembled", "by", "both", "systems", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["In", "the", "five-protein", "system", ",", "Hip", "prevents", "inhibition", "of", "assembly", "by", "the", "hsp70", "co-chaperone", "BAG-1", ",", "and", "cotransfection", "of", "Hip", "with", "BAG-1", "opposes", "BAG-1", "reduction", "of", "steroid", "binding", "activity", "in", "COS", "cells", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["We", "conclude", "that", "Hip", "is", "not", "a", "component", "of", "the", "assembly", "machinery", "but", "that", "it", "could", "play", "a", "regulatory", "role", "in", "opposition", "to", "BAG-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cutting", "edge", ":", "STAT6-deficient", "mice", "have", "enhanced", "tumor", "immunity", "to", "primary", "and", "metastatic", "mammary", "carcinoma", "."]} {"tags": [3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["STAT4", "and", "STAT6", "are", "essential", "for", "the", "development", "of", "CD4", "(", "+", ")", "Th1", "and", "Th2", "development", ",", "respectively", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Tumor", "immunologists", "have", "hypothesized", "that", "Th1", "cells", "are", "critical", "in", "tumor", "immunity", "because", "they", "facilitate", "differentiation", "of", "CD8", "(", "+", ")", "T", "cells", ",", "which", "are", "potent", "anti-tumor", "effectors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "used", "STAT4", "(", "-/-", ")", "and", "STAT6", "(", "-/-", ")", "mice", "to", "test", "this", "hypothesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["BALB/c", "and", "knockout", "mice", "were", "challenged", "in", "the", "mammary", "gland", "with", "the", "highly", "malignant", "and", "spontaneously", "metastatic", "BALB/c-derived", "4T1", "mammary", "carcinoma", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Primary", "tumor", "growth", "and", "metastatic", "disease", "are", "reduced", "in", "STAT6", "(", "-/-", ")", "mice", "relative", "to", "BALB/c", "and", "STAT4", "(", "-/-", ")", "mice", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ab", "depletions", "demonstrate", "that", "the", "effect", "is", "mediated", "by", "CD8", "(", "+", ")", "T", "cells", ",", "and", "immunized", "STAT6", "(", "-/-", ")", "mice", "have", "higher", "levels", "of", "4T1-specific", "CTL", "than", "BALB/c", "or", "STAT4", "(", "-/-", ")", "mice", "."]} {"tags": [0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Surprisingly", ",", "Th1", "or", "Th2", "cells", "are", "not", "involved", ",", "because", "CD4", "depletion", "does", "not", "diminish", "the", "anti-tumor", "effect", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", ",", "deletion", "of", "the", "STAT6", "gene", "facilitates", "development", "of", "potent", "anti-tumor", "immunity", "via", "a", "CD4", "(", "+", ")", "-independent", "pathway", "."]} {"tags": [0, 0, 3, 4, 4, 4, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Activation", "of", "oncogenic", "transcription", "factor", "AP-1", "in", "T", "cells", "infected", "with", "human", "T", "cell", "leukemia", "virus", "type", "1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 0, 0, 0], "tokens": ["Human", "T", "cell", "leukemia", "virus", "type", "1", "(", "HTLV-1", ")", "Tax", "protein", "transforms", "primary", "human", "T", "cells", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["We", "previously", "showed", "that", "Tax", "induces", "the", "expression", "of", "various", "family", "members", "of", "the", "transcription", "factor", "AP-1", "such", "as", "c-Jun", ",", "JunD", ",", "c-Fos", ",", "and", "Fra-1", "at", "the", "mRNA", "level", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 0, 0], "tokens": ["In", "this", "study", ",", "we", "have", "examined", "the", "ability", "of", "Tax", "to", "activate", "transcription", "through", "the", "AP-1-binding", "site", "(", "AP-1", "site", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 1, 2, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0], "tokens": ["A", "transient", "transfection", "study", "showed", "that", "Tax", "can", "activate", "transcription", "through", "the", "AP-1-binding", "site", "in", "a", "human", "T", "cell", "line", ",", "whereas", "any", "combination", "of", "AP-1", "proteins", "did", "so", "much", "less", "than", "Tax", ",", "indicating", "that", "the", "activation", "of", "the", "AP-1", "site", "by", "Tax", "may", "require", "a", "mechanism", "other", "than", "the", "induction", "of", "AP-1", "mRNA", "."]} {"tags": [5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Fresh", "peripheral", "blood", "leukemia", "cells", "of", "all", "surveyed", "ATL", "patients", "displayed", "constitutive", "AP-1", "DNA-binding", "activity", ",", "whereas", "no", "normal", "individuals", "did", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["However", ",", "the", "HTLV-1", "genes", ",", "including", "tax", ",", "are", "not", "significantly", "expressed", "in", "fresh", "leukemia", "cells", "from", "ATL", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Our", "present", "results", "suggest", "that", "activation", "of", "AP-1", "occurs", "through", "Tax-dependent", "and", "-independent", "mechanisms", "in", "HTLV-1-infected", "T", "cells", ",", "which", "may", "play", "some", "roles", "in", "dysregulated", "phenotypes", "of", "HTLV-1-infected", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["2", ",", "2", "'", ",", "4", ",", "6", ",", "6'-pentachlorobiphenyl", "induces", "apoptosis", "in", "human", "monocytic", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Polychlorinatedbiphenyls", "(", "PCBs", ")", "are", "a", "group", "of", "persistent", "and", "widely", "dispersed", "environmental", "pollutants", ",", "some", "of", "which", "may", "be", "immunotoxic", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0], "tokens": ["In", "the", "present", "study", ",", "we", "investigated", "the", "effect", "of", "PCBs", "on", "immune", "system", "by", "assessing", "apoptotic", "cell", "death", "in", "human", "monocytic", "U937", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Among", "the", "various", "congeners", "tested", ",", "2", ",", "2", "'", ",", "4", ",", "6", ",", "6'-pentachlorobiphenyl", "(", "PeCB", ")", ",", "a", "highly", "ortho-substituted", "congener", ",", "specifically", "induced", "DNA", "fragmentation", ",", "a", "hallmark", "of", "apoptosis", ",", "while", "the", "other", "examined", "di-", ",", "tri-", ",", "tetra-", ",", "and", "pentachlorobiphenyls", "did", "not", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "further", "study", "the", "2", ",", "2", "'", ",", "4", ",", "6", ",", "6'-PeCB-induced", "cell", "death", ",", "various", "features", "of", "apoptosis", "were", "examined", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["2", ",", "2", "'", ",", "4", ",", "6", ",", "6'-PeCB", "caused", "a", "decrease", "in", "cell", "viability", "and", "induced", "cellular", "morphologic", "features", "characteristic", "of", "apoptosis", "such", "as", "chromatin", "aggregation", "and", "apoptotic", "bodies", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "caspase-3", ",", "an", "executioner", "of", "apoptosis", ",", "was", "activated", "and", "its", "substrate", ",", "poly", "(", "ADP-ribose", ")", "polymerase", "(", "PARP", ")", ",", "was", "cleaved", "during", "2", ",", "2", "'", ",", "4", ",", "6", ",", "6'-PeCB-induced", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 3, 4, 0, 7, 8, 8, 8, 8, 0], "tokens": ["In", "contrast", ",", "3", ",", "3", "'", ",", "4", ",", "4", "'", ",", "5-PeCB", ",", "a", "congener", "of", "coplanar", "structure", ",", "as", "well", "as", "2", ",", "3", ",", "7", ",", "8-TCDD", "did", "not", "induce", "apoptosis", "in", "these", "human", "monocytic", "cells", ",", "although", "they", "potently", "induced", "CYP", "1A1", "in", "human", "hepatoma", "Hep", "G2", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Taken", "together", ",", "the", "data", "indicate", "that", "2", ",", "2", "'", ",", "4", ",", "6", ",", "6'-PeCB", "induces", "apoptosis", "in", "human", "monocytic", "cells", "through", "a", "mechanism", "that", "is", "independent", "of", "the", "arylhydrocarbon", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "suggests", "a", "possibly", "separate", "mechanism", "by", "which", "PCBs", "cause", "immunosuppression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Effects", "of", "deregulated", "Raf", "activation", "on", "integrin", ",", "cytokine-receptor", "expression", "and", "the", "induction", "of", "apoptosis", "in", "hematopoietic", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["The", "effects", "of", "deregulated", "Raf", "activation", "on", "the", "growth", "and", "differentiation", "of", "hematopoietic", "cells", "were", "investigated", "."]} {"tags": [0, 7, 8, 8, 8, 0, 7, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["The", "cytokine-dependent", "murine", "myeloid", "FDC-P1", "and", "human", "erythroleukemic", "TF-1", "cell", "lines", "were", "transformed", "to", "grow", "in", "response", "to", "deregulated", "Raf", "expression", "in", "the", "absence", "of", "exogenous", "cytokines", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 1, 0, 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 0, 3, 4, 4, 0, 0], "tokens": ["The", "conditionally", "active", "Raf", "proteins", "were", "regulated", "by", "beta-estradiol", "as", "cDNAs", "containing", "the", "Raf", "catalytic", ",", "but", "lacking", "negative-regulatory", "domains", ",", "were", "ligated", "to", "the", "hormone", "binding", "domain", "of", "the", "estrogen", "receptor", "(", "deltaRaf", ":", "ER", ")", "."]} {"tags": [0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Continuous", "deltaRaf", "expression", "prevented", "apoptosis", "in", "the", "absence", "of", "exogenous", "cytokines", "and", "altered", "the", "morphology", "of", "the", "FD/deltaRaf", ":", "ER", "cells", "as", "they", "grew", "in", "large", "aggregated", "masses", "(", ">", "100", "cells", ")", "whereas", "the", "parental", "cytokine-dependent", "FDC-P1", "cells", "grew", "in", "smaller", "grape-like", "clusters", "(", "<", "10", "cells", ")", "."]} {"tags": [7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 5, 6, 0], "tokens": ["FD/deltaRaf-1", ":", "ER", "cells", "growing", "in", "response", "to", "Raf", "activation", "displayed", "decreased", "levels", "of", "the", "Mac-2", "and", "Mac-3", "molecules", "on", "their", "cell", "surface", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["In", "contrast", ",", "when", "these", "cells", "were", "cultured", "in", "IL-3", ",", "higher", "levels", "of", "these", "adhesion", "molecules", "were", "detected", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["Expression", "of", "activated", "Raf", "oncoproteins", "also", "abrogated", "cytokine", "dependency", "and", "prevented", "apoptosis", "of", "TF-1", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 5, 6, 0], "tokens": ["Moreover", ",", "the", "differentiation", "status", "of", "these", "Raf-responsive", "cells", "was", "more", "immature", "upon", "Raf", "activation", "as", "culture", "with", "the", "differentiation-inducing", "agent", "phorbol", "12", "myristate", "13-acetate", "(", "PMA", ")", "and", "beta-estradiol", "resulted", "in", "decreased", "levels", "of", "the", "CD11b", "and", "CD18", "integrin", "molecules", "on", "the", "cell", "surface", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0], "tokens": ["In", "contrast", "when", "the", "Raf-responsive", "cells", "were", "induced", "to", "differentiate", "with", "PMA", "and", "GM-CSF", ",", "in", "the", "absence", "of", "deltaRaf", ":", "ER", "activation", ",", "increased", "levels", "of", "the", "CD11b", "and", "CD18", "molecules", "were", "detected", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Retinoic", "acid", "(", "RA", ")", "inhibited", "3H-thymidine", "incorporation", "in", "response", "to", "GM-CSF", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interestingly", ",", "Raf", "activation", "counterbalanced", "the", "inhibition", "of", "DNA", "synthesis", "caused", "by", "RA", "but", "not", "PMA", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", "deregulated", "Raf", "expression", "can", "alter", "cytokine", "dependency", ",", "integrin", "expression", "and", "the", "stage", "of", "differentiation", "."]} {"tags": [0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["These", "Raf-responsive", "cell", "lines", "will", "be", "useful", "in", "elucidating", "the", "roles", "of", "the", "MAP", "kinase", "cascade", "on", "hematopoietic", "cell", "differentiation", "and", "malignant", "transformation"]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 5, 6, 0, 0, 0, 3, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["Cyclic", "AMP", "activates", "p38", "mitogen-activated", "protein", "kinase", "in", "Th2", "cells", ":", "phosphorylation", "of", "GATA-3", "and", "stimulation", "of", "Th2", "cytokine", "gene", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["cAMP", "is", "an", "important", "second", "messenger", "with", "immunomodulatory", "properties", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["Elevation", "of", "intracellular", "cAMP", "in", "T", "cells", ",", "induced", "by", "agents", "such", "as", "IL-1alpha", "or", "PGs", ",", "inhibits", "T", "cell", "activation", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 0, 0, 3, 0, 0, 5, 6, 0], "tokens": ["In", "effector", "T", "cells", ",", "an", "increase", "in", "the", "level", "of", "intracellular", "cAMP", "inhibits", "cytokine", "production", "in", "Th1", "cells", "but", "stimulates", "cytokine", "production", "in", "Th2", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "report", "that", "cAMP-induced", "effects", "in", "Th2", "cells", "occur", "independently", "of", "the", "protein", "kinase", "A", "pathway", ",", "which", "is", "the", "major", "mediator", "of", "cAMP-induced", "signaling", "events", "in", "most", "cell", "types", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 5, 6, 0], "tokens": ["Instead", ",", "cAMP", "stimulates", "activation", "of", "p38", "mitogen-activated", "protein", "kinase", "in", "Th2", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 0], "tokens": ["This", "appears", "to", "be", "a", "Th2", "-selective", "event", "because", "cAMP", "barely", "increased", "p38", "phosphorylation", "in", "Th1", "cells", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "show", "that", "in", "Th2", "cells", ",", "cAMP", "promotes", "the", "production", "of", "both", "IL-5", "and", "IL-13", ",", "which", "play", "distinct", "but", "critical", "roles", "in", "asthma", "pathogenesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Our", "data", "also", "show", "that", "cAMP", "causes", "increased", "phosphorylation", "of", "the", "transcription", "factor", "GATA-3", ",", "which", "we", "have", "shown", "is", "a", "critical", "regulator", "of", "Th2", "cytokine", "gene", "expression", "and", ",", "in", "turn", ",", "of", "airway", "inflammation", "in", "mice", "."]} {"tags": [0, 0, 5, 0, 3, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Thus", ",", "Th2", "-specific", "GATA-3", "expression", "and", "p38", "mitogen-activated", "protein", "kinase", "activation", "together", "provide", "a", "molecular", "basis", "for", "the", "differential", "effects", "of", "cAMP", "in", "the", "two", "T", "helper", "cell", "subsets", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Characterization", "of", "IL-4", "and", "IL-13", "signals", "dependent", "on", "the", "human", "IL-13", "receptor", "alpha", "chain", "1", ":", "redundancy", "of", "requirement", "of", "tyrosine", "residue", "for", "STAT3", "activation", "."]} {"tags": [3, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["IL-4", "and", "IL-13", "are", "pleiotropic", "cytokines", "whose", "biological", "activities", "overlap", "with", "each", "other", "."]} {"tags": [3, 4, 4, 4, 4, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 4, 0, 0, 0, 0, 0], "tokens": ["IL-13", "receptor", "alpha", "chain", "1", "(", "IL-13R", "alpha", "1", ")", "is", "necessary", "for", "binding", "to", "IL-13", ",", "and", "the", "heterodimer", "composed", "of", "IL-13R", "alpha", "1", "and", "IL-4R", "alpha", "chain", "transduces", "IL-13", "and", "IL-4", "signals", ";", "however", ",", "the", "functional", "mapping", "of", "the", "intracellular", "domain", "of", "IL-13R", "alpha", "1", "is", "not", "fully", "understood", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 4, 4, 0, 7, 8, 8, 8, 0], "tokens": ["In", "this", "study", ",", "we", "constructed", "wild", "and", "mutated", "types", "of", "human", "IL-13R", "alpha", "1", ",", "and", "analyzed", "IL-4", "and", "IL-13", "signals", "using", "an", "IL-13R", "alpha", "1", "-transfected", "human", "B", "cell", "line", "."]} {"tags": [0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 0, 3, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0], "tokens": ["Expression", "of", "IL-13R", "alpha", "1", "evoked", "STAT3", "activation", "by", "IL-4", "and", "IL-13", ",", "and", "in", "stimulated", "human", "B", "cells", ",", "on", "which", "IL-13R", "alpha", "1", "was", "highly", "expressed", ",", "IL-4", "and", "IL-13", "induced", "STAT3", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Replacement", "of", "the", "two", "tyrosine", "residues", "completely", "abolished", "STAT3", "activation", ",", "although", "replacing", "either", "tyrosine", "residue", "alone", "retained", "it", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0], "tokens": ["Furthermore", ",", "we", "found", "that", "the", "Box1", "region", "and", "the", "C-terminal", "tail", "of", "IL-13R", "alpha", "1", "were", "critical", "for", "binding", "to", "Tyk2", ",", "and", "activation", "of", "Jak1", ",", "Tyk2", ",", "the", "insulin", "receptor", "substrate-1", "and", "STAT6", "respectively", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 3, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 0], "tokens": ["These", "results", "suggest", "that", "STAT3", "activation", "is", "involved", "with", "IL-4", "and", "IL-13", "signals", "in", "human", "B", "cells", "along", "with", "the", "activation", "of", "STAT6", ",", "and", "that", "there", "is", "a", "unique", "sequence", "in", "IL-13R", "alpha", "1", "to", "activate", "STAT3", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Functional", "uncoupling", "of", "the", "Janus", "kinase", "3-Stat5", "pathway", "in", "malignant", "growth", "of", "human", "T", "cell", "leukemia", "virus", "type", "1-transformed", "human", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Human", "T", "cell", "leukemia", "virus", "type", "1", "(", "HTLV-1", ")", "transforms", "cytokine", "-dependent", "T", "lymphocytes", "and", "causes", "adult", "T", "cell", "leukemia", "."]} {"tags": [3, 4, 4, 4, 4, 4, 4, 0, 3, 4, 3, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Janus", "tyrosine", "kinase", "(", "Jak", ")", "3", "and", "transcription", "factors", "Stat5a", "and", "Stat5b", "are", "essential", "for", "the", "proliferation", "of", "normal", "T", "cells", "and", "are", "constitutively", "hyperactivated", "in", "both", "HTLV-1-transformed", "human", "T", "cell", "lines", "and", "lymphocytes", "isolated", "from", "HTLV-1-infected", "patients", ";", "therefore", ",", "a", "critical", "role", "for", "the", "Jak3-Stat5", "pathway", "in", "the", "progression", "of", "this", "disease", "has", "been", "postulated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 7, 8, 8, 8, 0], "tokens": ["We", "recently", "reported", "that", "tyrphostin", "AG-490", "selectively", "blocked", "IL-2", "activation", "of", "Jak3/Stat5", "and", "growth", "of", "murine", "T", "cell", "lines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 7, 0, 7, 0], "tokens": ["Here", "we", "demonstrate", "that", "disruption", "of", "Jak3/Stat5a/b", "signaling", "with", "AG-490", "(", "50", "&", "mgr", ";", "M", ")", "blocked", "the", "proliferation", "of", "primary", "human", "T", "lymphocytes", ",", "but", "paradoxically", "failed", "to", "inhibit", "the", "proliferation", "of", "HTLV-1-transformed", "human", "T", "cell", "lines", ",", "HuT-102", "and", "MT-2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 5, 6, 0], "tokens": ["Structural", "homologues", "of", "AG-490", "also", "inhibited", "the", "proliferation", "of", "primary", "human", "T", "cells", ",", "but", "not", "HTLV-1-infected", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Disruption", "of", "constitutive", "Jak3/Stat5", "activation", "by", "AG-490", "was", "demonstrated", "by", "inhibition", "of", "1", ")", "tyrosine", "phosphorylation", "of", "Jak3", ",", "Stat5a", "(", "Tyr", "(", "694", ")", ")", ",", "and", "Stat5b", "(", "Tyr", "(", "699", ")", ")", ";", "2", ")", "serine", "phosphorylation", "of", "Stat5a", "(", "Ser", "(", "726", ")", ")", "as", "determined", "by", "a", "novel", "phosphospecific", "Ab", ";", "and", "3", ")", "Stat5a/b", "DNA", "binding", "to", "the", "Stat5-responsive", "beta-casein", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 3, 4, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["In", "contrast", ",", "AG-490", "had", "no", "effect", "on", "DNA", "binding", "by", "p50/p65", "components", "of", "NF-kappaB", ",", "a", "transcription", "factor", "activated", "by", "the", "HTLV-1-encoded", "phosphoprotein", ",", "Tax", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Collectively", ",", "these", "data", "suggest", "that", "the", "Jak3-Stat5", "pathway", "in", "HTLV-1-transformed", "T", "cells", "has", "become", "functionally", "redundant", "for", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Reversal", "of", "this", "functional", "uncoupling", "may", "be", "required", "before", "Jak3/Stat5", "inhibitors", "will", "be", "useful", "in", "the", "treatment", "of", "this", "malignancy", "."]} {"tags": [0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 0], "tokens": ["The", "Epstein-Barr", "virus", "promoter", "initiating", "B-cell", "transformation", "is", "activated", "by", "RFX", "proteins", "and", "the", "B-cell-specific", "activator", "protein", "BSAP/Pax5", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Epstein-Barr", "virus", "(", "EBV", ")", "-induced", "B-cell", "growth", "transformation", ",", "a", "central", "feature", "of", "the", "virus", "'", "strategy", "for", "colonizing", "the", "human", "B-cell", "system", ",", "requires", "full", "virus", "latent", "gene", "expression", "and", "is", "initiated", "by", "transcription", "from", "the", "viral", "promoter", "Wp", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interestingly", ",", "when", "EBV", "accesses", "other", "cell", "types", ",", "this", "growth-transforming", "program", "is", "not", "activated", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 5, 0, 0, 0], "tokens": ["The", "present", "work", "focuses", "on", "a", "region", "of", "Wp", "which", "in", "reporter", "assays", "confers", "B-cell", "-specific", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Bandshift", "studies", "indicate", "that", "this", "region", "contains", "three", "factor", "binding", "sites", ",", "termed", "sites", "B", ",", "C", ",", "and", "D", ",", "in", "addition", "to", "a", "previously", "characterized", "CREB", "site", "."]} {"tags": [0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 1, 2, 2, 2, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["Here", "we", "show", "that", "site", "C", "binds", "members", "of", "the", "ubiquitously", "expressed", "RFX", "family", "of", "proteins", ",", "notably", "RFX1", ",", "RFX3", ",", "and", "the", "associated", "factor", "MIBP1", ",", "whereas", "sites", "B", "and", "D", "both", "bind", "the", "B-cell-specific", "activator", "protein", "BSAP/Pax5", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 1, 2, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["In", "reporter", "assays", "with", "mutant", "Wp", "constructs", ",", "the", "loss", "of", "factor", "binding", "to", "any", "one", "of", "these", "sites", "severely", "impaired", "promoter", "activity", "in", "B", "cells", ",", "while", "the", "wild-type", "promoter", "could", "be", "activated", "in", "non-B", "cells", "by", "ectopic", "BSAP", "expression", "."]} {"tags": [0, 0, 0, 1, 0, 0, 3, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "suggest", "that", "Wp", "regulation", "by", "BSAP", "helps", "to", "ensure", "the", "B-cell", "specificity", "of", "EBV", "'s", "growth-transforming", "function", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Activation", "of", "the", "Lck", "tyrosine", "protein", "kinase", "by", "the", "Herpesvirus", "saimiri", "tip", "protein", "involves", "two", "binding", "interactions", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["The", "Tip", "protein", "of", "Herpesvirus", "saimiri", "strain", "484C", "binds", "to", "and", "activates", "the", "Lck", "tyrosine", "protein", "kinase", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Two", "sequences", "in", "the", "Tip", "protein", "were", "previously", "shown", "to", "be", "involved", "in", "binding", "to", "Lck", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0], "tokens": ["A", "proline-rich", "region", ",", "residues", "132-141", ",", "binds", "to", "the", "SH3", "domain", "of", "the", "Lck", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["We", "show", "here", "that", "the", "other", "Lck-binding", "domain", ",", "residues", "104-113", ",", "binds", "to", "the", "carboxyl-terminal", "half", "of", "Lck", "and", "that", "this", "binding", "does", "not", "require", "the", "Lck", "SH3", "domain", "."]} {"tags": [0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Mutated", "Tip", "containing", "only", "one", "functional", "Lck-binding", "domain", "can", "bind", "stably", "to", "Lck", ",", "although", "not", "as", "strongly", "as", "wild-type", "Tip", "."]} {"tags": [0, 0, 3, 0, 3, 0, 0, 3, 4, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Interaction", "of", "Tip", "with", "Lck", "through", "either", "Lck-binding", "domain", "increases", "the", "activity", "of", "Lck", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Simultaneous", "binding", "of", "both", "domains", "is", "required", "for", "maximal", "activation", "of", "Lck", "."]} {"tags": [0, 0, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0], "tokens": ["The", "transient", "expression", "of", "Tip", "in", "T", "cells", "was", "found", "to", "stimulate", "both", "Stat3", "-dependent", "and", "NF-AT", "-dependent", "transcription", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["Mutant", "forms", "of", "Tip", "lacking", "one", "or", "the", "other", "of", "the", "two", "Lck-binding", "domains", "retained", "the", "ability", "to", "stimulate", "Stat3", "-dependent", "transcription", "."]} {"tags": [3, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tip", "lacking", "the", "proline-rich", "Lck-binding", "domain", "exhibited", "almost", "wild-type", "activity", "in", "this", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0], "tokens": ["In", "contrast", ",", "ablation", "of", "either", "Lck-binding", "domain", "abolished", "the", "ability", "of", "Tip", "to", "stimulate", "NF-AT", "-dependent", "transcription", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Full", "biological", "activity", "of", "Tip", ",", "therefore", ",", "appears", "to", "require", "both", "Lck-binding", "domains", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Accumulation", "of", "RXR", "alpha", "during", "activation", "of", "cycling", "human", "T", "lymphocytes", ":", "modulation", "of", "RXRE", "transactivation", "function", "by", "mitogen-activated", "protein", "kinase", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0], "tokens": ["We", "have", "previously", "reported", "that", "the", "activation", "of", "resting", "human", "immature", "peripheral", "blood", "T", "(", "PBT", ")", "lymphocytes", "is", "associated", "with", "the", "loss", "of", "retinoid", "X", "receptor", "alpha", "(", "RXRalpha", ")", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 7, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["In", "the", "present", "study", ",", "we", "have", "demonstrated", "that", ",", "unlike", "resting", "cells", ",", "activation", "of", "cycling", "human", "mature", "PBT", "lymphocytes", ",", "and", "T", "lymphocyte", "leukemia", "cell", "lines", "is", "accompanied", "by", "the", "accumulation", "of", "RXRalpha", "mRNA", "and", "protein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interestingly", ",", "cyclosporin", "A", "further", "augmented", "RXRalpha", "expression", ",", "indicating", "the", "involvement", "of", "calcineurin", "pathways", "in", "the", "process", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["9-cis", "retinoic", "acid", "inhibited", "the", "accumulation", ",", "suggesting", "that", "retinoids", "can", "regulate", "the", "synthesis", "of", "their", "own", "receptors", "during", "T", "cell", "activation", "."]} {"tags": [0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transfection", "analysis", "in", "Jurkat", "cells", ",", "using", "RXRE-dependent", "reporter", "assays", ",", "showed", "that", "RXRalpha", "accumulated", "during", "T", "cell", "activation", "was", "transcriptionally", "inactive", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "investigate", "the", "mechanism", "of", "such", "inhibition", ",", "the", "role", "of", "two", "mitogen-activated", "protein", "kinase", "pathways", ",", "c-Jun", "N-terminal", "kinase", "(", "JNK", ")", "and", "extracellular", "signal-regulated", "kinase", "(", "ERK", ")", ",", "in", "modulating", "RXRE-dependent", "transcription", ",", "was", "explored", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["The", "expression", "of", "constitutively", "active", "MAP/ERK", "kinase", "kinase", "1", "(", "MEKK1", ")", "inhibited", "RXRE-dependent", "transcription", ",", "whereas", "dominant", "negative", "MEKK1", "increased", "the", "transcription", ",", "indicating", "the", "involvement", "of", "JNK", "signaling", "pathways", "in", "the", "process", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "expression", "of", "constitutively", "active", "MEK1", ",", "which", "activates", "ERK", "pathway", ",", "enhanced", "RXRE-dependent", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["When", "both", "were", "activated", "simultaneously", ",", "JNK", "pathway", "was", "dominant", "over", "ERK", "pathway", "and", "resulted", "in", "inhibition", "of", "RXRE-mediated", "transcription", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "demonstrate", "a", "dual", "regulatory", "control", "of", "RXRalpha", "expression", "during", "the", "activation", "of", "resting", "and", "cycling", "T", "lymphocytes", "and", "indicate", "a", "dynamic", "balance", "between", "JNK", "and", "ERK", "pathways", "in", "modulating", "RXRE-mediated", "transactivation", "."]} {"tags": [0, 3, 0, 0, 0, 0, 3, 0], "tokens": ["The", "proteasome", "regulates", "receptor-mediated", "endocytosis", "of", "interleukin-2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Recent", "studies", "have", "increasingly", "implicated", "the", "proteasome", "in", "the", "regulation", "of", "cell", "surface", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0], "tokens": ["In", "the", "present", "study", ",", "we", "investigated", "the", "role", "of", "the", "proteasome", "for", "ligand-dependent", "endocytosis", "and", "degradation", "of", "the", "interleukin-2", "(", "IL-2", ")", "-interleukin-2", "receptor", "(", "IL-2R", ")", "complex", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Proteasome", "inhibitors", "impaired", "internalization", "of", "IL-2.IL-2R", "and", "prevented", "the", "lysosomal", "degradation", "of", "this", "cytokine", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Based", "on", "time-course", "studies", ",", "proteasome", "activity", "is", "primarily", "required", "after", "initial", "endocytosis", "of", "the", "IL-2.IL-2R", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 3, 4, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Proteasome", "function", "was", "also", "necessary", "for", "the", "lysosomal", "degradation", "of", "IL-2", "internalized", "by", "IL-2R", "that", "were", "comprised", "of", "cytoplasmic", "tailless", "beta-", "or", "gamma", "c-subunits", ",", "suggesting", "that", "the", "target", "protein", "for", "the", "proteasome", "is", "independent", "of", "either", "the", "cytoplasmic", "tail", "of", "the", "IL-2R", "beta-", "or", "gamma", "c-subunits", "and", "their", "associated", "signaling", "components", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", ",", "a", "functional", "proteasome", "is", "required", "for", "optimal", "endocytosis", "of", "the", "IL-2R/ligand", "complex", "and", "is", "essential", "for", "the", "subsequent", "lysosomal", "degradation", "of", "IL-2", ",", "possibly", "by", "regulating", "trafficking", "to", "the", "lysosome", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["Functional", "characterization", "of", "the", "two", "alternative", "promoters", "of", "human", "p45", "NF-E2", "gene", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 4, 0, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 5, 0, 0, 0], "tokens": ["OBJECTIVE", ":", "The", "transcription", "factor", "NF-E2", ",", "a", "heterodimeric", "protein", "complex", "composed", "of", "p45", "and", "small", "Maf", "family", "proteins", ",", "is", "considered", "crucial", "for", "the", "proper", "differentiation", "of", "erythrocytes", "and", "megakaryocytes", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 5, 6, 0], "tokens": ["We", "report", "the", "results", "of", "studies", "aimed", "at", "understanding", "the", "regulatory", "mechanisms", "controlling", "p45", "gene", "expression", "in", "erythroid", "cells", "."]} {"tags": [0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["MATERIALS", "AND", "METHODS", ":", "Human", "p45", "mRNAs", "have", "two", "alternative", "isoforms", ",", "aNF-E2", "and", "fNF-E2", ",", "and", "these", "isoforms", "are", "transcribed", "from", "the", "alternative", "promoters", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "investigated", "lineage-specific", "expression", "of", "both", "isomers", "in", "human", "erythroid", "and", "megakaryocytic", "cells", "by", "reverse", "transcriptase", "polymerase", "chain", "reaction", "or", "Northern", "blot", "analysis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 7, 8, 8, 8, 0], "tokens": ["For", "functional", "characterization", "of", "both", "promoters", ",", "plasmids", "in", "which", "reporter", "genes", "were", "placed", "under", "the", "control", "of", "a", "series", "of", "truncated", "or", "mutated", "promoter", "fragments", "were", "transfected", "to", "human", "hematopoietic", "cell", "lines", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["RESULTS", ":", "When", "CD34", "(", "+", ")", "cells", "isolated", "from", "human", "cord", "blood", "were", "induced", "to", "unilineage", "erythroid", "or", "megakaryocytic", "differentiation", "in", "liquid", "suspension", "culture", ",", "both", "transcripts", ",", "although", "barely", "detected", "at", "day", "0", ",", "were", "induced", "in", "both", "erythroid", "and", "megakaryocytic", "cultures", "."]} {"tags": [9, 10, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["fNF-E2", "mRNA", "was", "found", "to", "be", "more", "abundant", "in", "erythroid", "cells", "than", "megakaryocytic", "cells", "at", "day", "7", "of", "culture", "."]} {"tags": [0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0], "tokens": ["Although", "both", "isomers", "were", "expressed", "in", "human", "erythroid-megakaryocytic", "cell", "lines", ",", "megakaryocytic", "maturation", "with", "loss", "of", "erythroid", "phenotype", "induced", "by", "phorbol", "12-myristate", "13-acetate", "(", "PMA", ")", "resulted", "in", "exclusive", "downregulation", "of", "fNF-E2", ",", "suggesting", "that", "fNF-E2", "promoter", "is", "more", "erythroid", "specific", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Functional", "analysis", "of", "fNF-E2", "promoter", "showed", "that", "the", "promoter", "is", "active", "only", "in", "erythroid-megakaryocytic", "cells", "and", "that", "the", "double", "GATA", "sit", "e", "in", "the", "proximal", "region", "is", "necessary", "for", "its", "efficient", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["CONCLUSION", ":", "These", "results", "suggest", "that", "GATA", "proteins", ",", "which", "govern", "the", "differentiation", "of", "erythroid", "lineage", "cells", ",", "are", "required", "for", "full", "promoter", "activity", "of", "the", "p45", "gene", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transcriptional", "activation", "of", "heme", "oxygenase-1", "and", "its", "functional", "significance", "in", "acetaminophen-induced", "hepatitis", "and", "hepatocellular", "injury", "in", "the", "rat", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["BACKGROUND/AIM", ":", "Glutathione", "depletion", "contributes", "to", "acetaminophen", "hepatotoxicity", "and", "is", "known", "to", "induce", "the", "oxidative", "stress", "reactant", "heme", "oxygenase-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "metabolites", "of", "the", "heme", "oxygenase", "pathway", ",", "biliverdin", ",", "carbon", "monoxide", ",", "and", "iron", "may", "modulate", "acetaminophen", "toxicity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "aim", "of", "this", "study", "was", "to", "assess", "cell-type", "specific", "expression", "of", "heme", "oxygenase-1", "and", "its", "impact", "on", "liver", "injury", "and", "microcirculatory", "disturbances", "in", "a", "model", "of", "acetaminophen-induced", "hepatitis", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["METHODS", ":", "Gene", "expression", "of", "heme", "oxygenase-1", "was", "studied", "by", "Northern-", "and", "Western", "analysis", "as", "well", "as", "immunohistochemistry", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["The", "time", "course", "of", "heme", "oxygenase-1", "and", "-2", ",", "cytokine-induced", "neutrophil", "chemoattractant-1", ",", "and", "intercellular", "adhesion", "molecule-1", "was", "studied", "by", "Northern", "analysis", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["DNA-binding", "activity", "of", "nuclear", "factor-kappaB", "was", "determined", "by", "electrophoretic", "mobility", "shift", "assay", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Sinusoidal", "perfusion", "and", "leukocyte-endothelial", "interactions", "were", "assessed", "by", "intravital", "microscopy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 3, 4, 0, 3, 4, 4, 0, 3, 4, 4, 0, 0], "tokens": ["RESULTS", ":", "Acetaminophen", "caused", "a", "moderate", "sinusoidal", "perfusion", "failure", "(", "-15", "%", ")", "and", "infiltration", "of", "neutrophils", "along", "with", "activation", "of", "nuclear", "factor-kappaB", "and", "intercellular", "adhesion", "molecule-1", "and", "cytokine-induced", "neutrophil", "chemoattractant-1", "mRNAs", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Induction", "of", "heme", "oxygenase-1", "mRNA", "and", "protein", "(", "approximately", "30-fold", ")", "in", "hepatocytes", "and", "non-parenchymal", "cells", "paralleled", "the", "inflammatory", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Blockade", "of", "heme", "oxygenase", "activity", "with", "tin-protoporphyrin-IX", "abrogated", "acetaminophen-induced", "hepatic", "neutrophil", "accumulation", "and", "nuclear", "factor-kappaB", "activation", ",", "but", "failed", "to", "affect", "sinusoidal", "perfusion", "and", "liver", "injury", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["CONCLUSIONS", ":", "The", "inflammatory", "response", "associated", "with", "acetaminophen", "hepatotoxicity", "is", "modulated", "by", "the", "parallel", "induction", "of", "the", "heme", "oxygenase-1", "gene", "."]} {"tags": [0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "heme", "oxygenase-1", "has", "no", "permissive", "effect", "on", "sinusoidal", "perfusion", "and", "does", "not", "affect", "liver", "injury", "in", "this", "model", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "data", "argue", "against", "a", "central", "role", "of", "nuclear", "factor-kappaB", "activation", "and", "neutrophil", "infiltration", "as", "perpetuating", "factors", "of", "liver", "injury", "in", "acetaminophen", "toxicity", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Tumor", "necrosis", "factor-alpha", "-induced", "proliferation", "requires", "synthesis", "of", "granulocyte-macrophage", "colony-stimulating", "factor", "."]} {"tags": [0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["OBJECTIVE", ":", "Tumor", "necrosis", "factor-", "alpha", "(", "TNF-alpha", ")", "induces", "a", "variety", "of", "cellular", "responses", ",", "some", "of", "them", "being", "at", "least", "seemingly", "contradictory", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Thus", ",", "we", "set", "out", "to", "find", "differences", "in", "the", "modes", "of", "proliferative", "and", "apoptotic", "responses", "to", "TNF-", "alpha", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 3, 4, 0, 0], "tokens": ["MATERIALS", "AND", "METHODS", ":", "We", "screened", "a", "panel", "of", "acute", "myeloid", "leukemia-derived", "cell", "lines", "for", "TNF-", "alpha", "-responsiveness", "."]} {"tags": [0, 0, 0, 0, 7, 0, 7, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 7, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "two", "lines", "(", "OCI-AML-1", ",", "OCI-AML-11", ")", ",", "TNF-", "alpha", "acted", "as", "an", "apoptotic", "agent", ";", "in", "others", "(", "HU-3", ",", "M-07e", ",", "TF-1", ")", ",", "it", "had", "the", "opposite", "effect", ",", "preventing", "apoptosis", "and", "inducing", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Direct", "and", "indirect", "signaling", "mechanisms", ",", "including", "NF-kappaB", "activation", "and", "cytokine", "synthesis", ",", "were", "analyzed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 4, 0], "tokens": ["RESULTS", ":", "All", "cell", "lines", "tested", "expressed", "TNF-", "alpha", "receptors", "I", "and", "II", "and", "responded", "to", "TNF-", "alpha", "by", "upregulation", "of", "intercellular", "adhesion", "molecule-1", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", "to", "granulocyte-macrophage", "colony-stimulating", "factor", "(", "GM-CSF", ")", ",", "TNF-", "alpha", "did", "not", "activate", "the", "MAP", "kinase", "and", "p70S6", "kinase", "pathways", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Nevertheless", ",", "inhibitors", "of", "these", "pathways", "clearly", "reduced", "the", "TNF-alpha", "-induced", "cell", "growth", ",", "indicating", "that", "TNF-", "alpha-proliferative", "cells", "produced", "a", "growth", "factor", "that", "induced", "proliferation", "upon", "stimulation", "of", "the", "above", "pathways", "."]} {"tags": [3, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Anti-GM-CSF", "antibodies", "inhibited", "the", "TNF-alpha", "-induced", "growth", ",", "suggesting", "the", "presence", "of", "an", "autocrine", "loop", "for", "cell", "proliferation", "mediated", "by", "GM-CSF", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Supporting", "this", "notion", ",", "TNF-alpha", "-induced", "upregulation", "of", "GM-CSF", "mRNA", "levels", "and", "protein", "secretion", "in", "the", "TNF-alpha", "-proliferative", ",", "but", "not", "in", "the", "TNF-alpha-apoptotic", "cell", "lines", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["CONCLUSION", ":", "These", "data", "identify", "GM-CSF", "synthesis", "as", "an", "early", "and", "essential", "step", "in", "TNF-", "alpha-induced", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "show", "for", "the", "first", "time", "that", "TNF-alpha-treated", "cell", "lines", "producing", "no", "or", "only", "minimal", "amounts", "of", "GM-CSF", "demonstrate", "an", "apoptotic", "phenotype", ",", "while", "cell", "lines", "with", "high", "GM-CSF", "expression", "rates", "can", "escape", "from", "growth", "arrest", "or", "even", "apoptosis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["In", "this", "context", ",", "we", "discuss", "arguments", "pointing", "at", "NF-kappaB", "as", "regulator", "of", "GM-CSF", "synthesis", "and", "thus", "indirectly", "as", "regulator", "for", "the", "escape", "of", "TNF-alpha", "-induced", "apoptosis"]} {"tags": [3, 4, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptor", "content", "of", "T", "lymphocytes", ":", "evidence", "for", "heterogeneity", "."]} {"tags": [3, 4, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptors", "were", "measured", "in", "T", "lymphocytes", "that", "were", "isolated", "from", "peripheral", "blood", "by", "either", "nylon", "wool", "filtration", "or", "E-rosette", "sedimentation", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T", "cells", "isolated", "by", "nylon", "wool", "filtration", "specifically", "bind", "6.7", "+/-", "0.2", "fmol", "of", "dexamethasone", "per", "million", "cells", "(", "equivalent", "to", "4000", "+/-", "200", "receptors", "per", "cell", ")", ",", "whereas", "T", "cells", "isolated", "by", "E-rosette", "sedimentation", "bind", "12.0", "+/-", "0.7", "fmol", "of", "dexamethasone", "per", "million", "cells", "(", "equivalent", "to", "7200", "+/-", "400", "receptors", "per", "cell", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "difference", "in", "the", "amount", "of", "dexamethasone", "bound", "by", "the", "two", "T", "cell", "preparations", "was", "significant", "(", "p", "less", "than", ".001", ")", "and", "was", "present", "immediately", "after", "cell", "isolation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0], "tokens": ["The", "binding", "affinities", "of", "the", "different", "T", "cell", "preparations", "for", "dexamethasone", "were", "similar", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["T", "cells", "that", "are", "isolated", "by", "a", "combination", "of", "nylon", "wool", "filtration", "followed", "by", "E-rosette", "sedimentation", "bind", "the", "same", "amount", "of", "dexamethasone", "as", "T", "cells", "isolated", "by", "nylon", "wool", "filtration", "alone", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["T", "cells", "isolated", "by", "a", "combination", "of", "E-rosette", "sedimentation", "following", "by", "nylon", "wool", "filtration", "bind", "less", "dexamethasone", "than", "do", "T", "cells", "isolated", "by", "E-rosette", "sedimentation", "alone", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["These", "findings", "suggest", "that", "T", "cells", "are", "heterogeneous", "with", "respect", "to", "their", "quantity", "of", "glucocorticoid", "receptors", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Isolation", "of", "T", "cells", "by", "E-rosette", "sedimentation", "enriches", "for", "T", "cells", "that", "have", "a", "greater", "number", "of", "glucocorticoid", "receptors", ",", "and", "isolation", "of", "T", "cells", "by", "nylon", "wool", "filtration", "enriches", "for", "T", "cells", "that", "have", "a", "lesser", "number", "of", "glucocorticoid", "receptors", "."]} {"tags": [3, 4, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Glucocorticoid", "receptors", "and", "glucocorticoid", "sensitivity", "of", "human", "leukemic", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 6, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "established", "optimal", "conditions", "for", "the", "measurement", "of", "glucocorticoid", "receptors", "(", "GR", ")", "in", "human", "white", "cells", "using", "a", "whole-cell", "binding", "assay", "with", "[", "3H", "]", "dexamethasone", "as", "the", "ligand", ",", "and", "the", "subsequent", "determination", "of", "the", "GR", "content", "in", "normal", "human", "lymphocytes", "and", "in", "leukemic", "cells", "of", "patients", "with", "various", "forms", "of", "acute", "and", "chronic", "leukemia", "."]} {"tags": [0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0], "tokens": ["A", "number", "of", "leukemia", "cell", "lines", "in", "continuous", "culture", "were", "also", "subjected", "to", "the", "GR", "assay", ",", "and", "the", "results", "were", "correlated", "with", "the", "sensitivity", "of", "these", "cell", "lines", "to", "glucocorticoid", "steroids", "in", "vitro", "."]} {"tags": [0, 3, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "GR", "content", "of", "normal", "human", "lymphocytes", "amounted", "to", "4", ",", "850", "+/-", "1", ",", "340", "(", "mean", "+/-", "SD", ")", "receptors/cell", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "mean", "equilibrium", "dissociation", "constant", "(", "KD", ")", "of", "the", "interaction", "of", "[", "3H", "]", "dexamethasone", "with", "the", "GR", "was", "1.2", "x", "10", "(", "-8", ")", "M", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Steroidal", "compounds", "with", "a", "known", "glucocorticoid", "potency", "effectively", "competed", "for", "the", "binding", ",", "whereas", "steroids", "devoid", "of", "glucocorticoid", "activity", "(", "e.g.", "estradiol-17", "beta", "and", "testosterone", ")", "were", "ineffective", "."]} {"tags": [0, 3, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["The", "GR", "content", "of", "the", "blast", "cells", "obtained", "from", "eight", "patients", "suffering", "from", "acute", "leukemia", "and", "four", "patients", "with", "a", "blast", "crisis", "of", "chronic", "myelocytic", "leukemia", "was", "found", "to", "be", "highly", "variable", "(", "3", ",", "230-29", ",", "900", "receptors/cell", ")", ",", "while", "the", "lymphocytes", "of", "six", "patients", "with", "chronic", "lymphatic", "leukemia", "contained", "a", "rather", "stable", "GR", "content", "(", "2", ",", "930-5", ",", "120", "receptors/cell", ")", ",", "which", "was", "comparable", "with", "that", "of", "normal", "lymphocytes", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 0], "tokens": ["GR", "was", "identified", "in", "all", "the", "12", "malignant", "continuous", "white", "cell", "lines", "studied", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Large", "cells", "contained", "more", "GR", "than", "the", "smaller", "ones", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["There", "was", "no", "apparent", "correlation", "between", "the", "GR", "concentration", "and", "the", "sensitivity", "of", "the", "cells", "in", "vitro", "to", "glucocorticoids", "as", "judged", "by", "[", "3H", "]", "thymidine", "incorporation", "studies", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Distribution", "of", "the", "surface", "markers", "in", "the", "leukemic", "cell", "lines", "did", "not", "relate", "to", "the", "GR", "concentration", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "conclude", "that", "the", "presence", "of", "GR", "is", "probably", "a", "universal", "feature", "of", "the", "leukemic", "cells", ",", "and", ",", "from", "a", "clinical", "standpoint", ",", "probably", "does", "not", "alone", "imply", "steroid", "responsiveness", "."]} {"tags": [0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 0], "tokens": ["Evidence", "for", "a", "steroid", "receptor", "in", "rheumatoid", "synovial", "tissue", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["One", "mechanism", "by", "which", "glucocorticoids", "could", "exert", "their", "anti-inflammatory", "action", "is", "via", "rapidly", "saturable", ",", "stereo-specific", "cytoplasmic", "protein", "receptors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["This", "report", "is", "of", "an", "investigation", "into", "such", "a", "possibility", "in", "synovial", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Synovium", ",", "obtained", "from", "knee", "joints", "of", "rheumatoid", "patients", "undergoing", "surgery", ",", "was", "incubated", "with", "clostridiopeptidase", "A", "and", "trypsin-EDTA", "to", "obtain", "cell", "suspensions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", ",", "together", "with", "cells", "obtained", "from", "synovial", "fluid", "aspirated", "from", "patients", "with", "rheumatoid", "arthritis", ",", "were", "identified", "by", "electron", "microscopy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Duplicate", "samples", "of", "these", "cell", "suspensions", "were", "incubated", "with", "increasing", "concentrations", "of", "H3Dexamethasone", "(", "1", "x", "10", "(", "-10", ")", "M-1", "x", "10", "(", "-9", ")", "M", ")", "for", "30", "minutes", "at", "37", "degrees", "C", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Analysis", "of", "the", "proportion", "of", "steroid", "bound", "to", "whole", "cells", "showed", "evidence", "for", "specific", ",", "rapidly", "saturable", ",", "receptors", "in", "the", "cells", "obtained", "from", "synovial", "tissue", ",", "but", "this", "was", "not", "found", "in", "synovial", "fluid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 5, 0, 5, 0, 5, 0, 5, 0], "tokens": ["Electron", "micrographs", "showed", "that", "cells", "obtained", "from", "synovial", "tissue", "consisted", "of", "synovial", "fibroblast", "-", "and", "macrophage-types", ",", "lymphocytes", ",", "monocytes", "and", "macrophages", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0], "tokens": ["Polymorphonuclear", "leucocytes", "appeared", "to", "be", "absent", "."]} {"tags": [0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "in", "synovial", "fluid", "cell", "type", "polymorphonuclear", "leucocytes", "were", "the", "predominant", "cell", "type", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["We", "concluded", "from", "this", ",", "that", "one", "or", "more", "of", "the", "cell", "types", "present", "in", "synovial", "tissue", "contain", "a", "specific", "steroid", "receptor", ",", "but", "that", "this", "is", "lacking", "in", "synovial", "fluid", "polymorphonuclear", "leucocytes", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["Clinical", "implications", "of", "glucocorticoid", "receptors", "in", "human", "leukemia", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 5, 6, 0], "tokens": ["Glucorticoid", "receptors", "were", "studied", "in", "various", "populations", "of", "normal", "human", "peripheral", "blood", "lymphocytes", "and", "leukemic", "lymphoblasts", "."]} {"tags": [5, 6, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Normal", "lymphocytes", "contain", "low", "levels", "of", "glucocorticoid", "receptor", "(", "approximately", "2", ",", "500", "sites/cell", ")", "which", "are", "identical", "in", "T-", "and", "non-T-fractions", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0], "tokens": ["Phytohemagglutinin", "treatment", "increases", "levels", "about", "3-fold", "."]} {"tags": [5, 6, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Leukemic", "lymphoblasts", "contain", "larger", "numbers", "of", "receptor", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Presence", "of", "receptor", "is", "correlated", "with", "in", "vitro", "sensitivitiy", "to", "glucocorticoids", "and", "in", "vivo", "response", "to", "therapy", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0], "tokens": ["Quantity", "of", "receptor", "is", "also", "correlated", "with", "complete", "remission", "duration", "independently", "of", "leukemic", "cell", "type", "(", "T", "or", "null", ")", ",", "initial", "WBC", ",", "or", "age", "of", "patient", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Quantitative", "determination", "of", "glucocorticoid", "receptor", "levels", "in", "acute", "lymphoblastic", "leukemia", "may", "be", "of", "value", "both", "as", "an", "independent", "prognostic", "variable", "and", "in", "suggesting", "which", "patients", "should", "receive", "glucocorticoid", "therapy", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["Functional", "and", "physical", "interaction", "of", "protein-tyrosine", "kinases", "Fyn", "and", "Csk", "in", "the", "T-cell", "signaling", "system", "."]} {"tags": [0, 3, 4, 4, 3, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "Src-like", "protein-tyrosine", "kinase", "Fyn", "is", "associated", "with", "T-cell", "antigen", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 1, 2, 2, 2, 2, 0, 1, 0, 0, 1, 2, 2, 0, 1, 2, 2, 2, 0, 0, 1, 2, 0], "tokens": ["Transient", "expression", "of", "actively", "mutated", "Fyn", ",", "having", "Phe-528", "instead", "of", "Tyr-528", "or", "Thr-338", "instead", "of", "Ile-338", ",", "in", "Jurkat", "T-cells", "stimulated", "the", "serum", "response", "element", "(", "SRE", ")", ",", "12-O-tetradecanoyl-phorbol-13-acetate", "response", "element", ",", "cyclic", "AMP", "response", "element", ",", "and", "c-fos", "promoter", "."]} {"tags": [0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["The", "stimulation", "of", "SRE", "was", "particularly", "prominent", "not", "only", "with", "active", "Fyn", "but", "also", "with", "normal", "(", "wild-type", ")", "Fyn", "."]} {"tags": [1, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["SRE", "was", "also", "stimulated", "by", "both", "normal", "and", "active", "Lck", "."]} {"tags": [0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["Furthermore", ",", "normal", "and", "active", "Fyn", "stimulated", "transcription", "from", "the", "IL-2", "gene", "promoter", "when", "transfected", "cells", "were", "stimulated", "by", "concanavalin", "A", "plus", "12-O-tetradecanoylphorbol-13-acetate", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Under", "the", "same", "conditions", ",", "Lck", "did", "not", "stimulate", "IL-2", "promoter", "unless", "it", "was", "activated", "by", "mutation", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["Interestingly", ",", "a", "mutant", "Fyn", ",", "which", "has", "deletions", "within", "the", "SH2", "region", "and", "so", "is", "able", "to", "transform", "chicken", "embryo", "fibroblasts", ",", "did", "not", "stimulate", "either", "the", "c-fos", "or", "IL-2", "promoter", ",", "suggesting", "the", "importance", "of", "this", "region", "in", "T-cell", "signaling", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 0, 0], "tokens": ["Csk", ",", "which", "phosphorylates", "tyrosine", "residues", "in", "the", "negative", "regulatory", "sites", "of", "Src", "family", "kinases", ",", "down-regulated", "Fyn-", "and", "Lck-mediated", "stimulation", "of", "the", "serum", "response", "element", "and", "Fyn-mediated", "enhancement", "of", "IL-2", "promoter", "activity", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["These", "data", "suggest", "that", "Fyn", "and", "Lck", ",", "whose", "activities", "are", "regulated", "by", "Csk", ",", "are", "involved", "in", "different", "phases", "of", "T-cell", "activation", "."]} {"tags": [0, 0, 3, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "novel", "NF-kappa", "B", "complex", "containing", "p65", "homodimers", ":", "implications", "for", "transcriptional", "control", "at", "the", "level", "of", "subunit", "dimerization", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 3, 4, 0, 0, 3, 4, 4, 0, 3, 4, 0, 3, 0], "tokens": ["The", "predominant", "inducible", "form", "of", "the", "NF-kappa", "B", "transcription", "factor", "is", "a", "heteromeric", "complex", "containing", "two", "Rel-related", "DNA-binding", "subunits", ",", "termed", "p65", "and", "p50", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Prior", "transfection", "studies", "have", "shown", "that", "when", "these", "p65", "and", "p50", "subunits", "are", "expressed", "independently", "as", "stable", "homodimers", ",", "p65", "stimulates", "kappa", "B-directed", "transcription", ",", "whereas", "p50", "functions", "as", "a", "kappa", "B-specific", "repressor", "."]} {"tags": [0, 0, 3, 4, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["While", "authentic", "p50", "homodimers", "(", "previously", "termed", "KBF1", ")", "have", "been", "detected", "in", "nuclear", "extracts", "from", "nontransfected", "cells", ",", "experimental", "evidence", "supporting", "the", "existence", "of", "p65", "homodimers", "in", "vivo", "was", "lacking", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 6, 0], "tokens": ["We", "now", "provide", "direct", "biochemical", "evidence", "for", "the", "presence", "of", "an", "endogenous", "pool", "of", "inducible", "p65", "homodimers", "in", "intact", "human", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["As", "with", "the", "prototypical", "NF-kappa", "B", "p50-p65", "heterodimer", ",", "this", "novel", "p65", "homodimeric", "form", "of", "NF-kappa", "B", "is", "functionally", "sequestered", "in", "the", "cytoplasm", "but", "rapidly", "appears", "in", "the", "nuclear", "compartment", "following", "cellular", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["Site-directed", "mutagenesis", "studies", "indicate", "that", "the", "homodimerization", "function", "of", "p65", "is", "dependent", "upon", "the", "presence", "of", "cysteine", "216", "and", "a", "conserved", "recognition", "motif", "for", "protein", "kinase", "A", "(", "RRPS", ";", "amino", "acids", "273", "to", "276", ")", ",", "both", "of", "which", "reside", "within", "a", "91-amino-acid", "segment", "of", "the", "Rel", "homology", "domain", "that", "mediates", "self-association", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "mutations", "at", "these", "two", "sites", "do", "not", "affect", "heterodimerization", "of", "p65", "with", "p50", "or", "its", "functional", "interaction", "with", "I", "kappa", "B", "alpha", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 3, 0], "tokens": ["These", "later", "findings", "indicate", "that", "neither", "homo-", "nor", "heterodimer", "formation", "is", "an", "absolute", "prerequisite", "for", "I", "kappa", "B", "alpha", "recognition", "of", "p65", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0], "tokens": ["Taken", "together", "with", "prior", "in", "vivo", "transcription", "studies", ",", "these", "results", "suggest", "that", "the", "biological", "activities", "of", "p65", "and", "p50", "homodimers", "are", "independently", "regulated", ",", "thereby", "providing", "an", "integrated", "and", "flexible", "control", "mechanism", "for", "the", "rapid", "activation", "and", "repression", "of", "NF-kappa", "B", "/", "Rel", "-directed", "gene", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Carrier", "determination", "for", "X-linked", "agammaglobulinemia", "using", "X", "inactivation", "analysis", "of", "purified", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "report", "the", "development", "of", "a", "relatively", "quick", "and", "simple", "method", "for", "the", "assessment", "of", "X", "inactivation", "status", "for", "carrier", "determination", "in", "families", "affected", "by", "X-linked", "agammaglobulinemia", "(", "XLA", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "method", "utilises", "an", "immunomagnetic", "separation", "technique", "for", "B", "cell", "purification", "and", "a", "polymerase", "chain", "reaction", "(", "PCR", ")", "based", "assay", "for", "the", "determination", "of", "methylation", "status", "at", "the", "androgen", "receptor", "(", "AR", ")", "gene", "locus", "to", "assess", "whether", "X", "inactivation", "is", "random", "or", "non-random", "at", "this", "locus", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "report", "the", "results", "we", "have", "obtained", "using", "this", "assay", "to", "investigate", "females", "known", "to", "be", "carriers", "of", "various", "X-linked", "immunodeficiency", "disorders", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "we", "investigated", "four", "females", "from", "different", "families", "affected", "by", "XLA", ",", "two", "of", "whom", "were", "of", "unknown", "carrier", "status", ",", "and", "we", "discuss", "the", "results", "obtained", "with", "this", "and", "other", "X-inactivation", "assays", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "similar", "assay", "has", "recently", "been", "described", "by", "Allen", "et", "al.", "(", "1992", ")", "and", "applied", "to", "members", "of", "one", "family", "affected", "by", "XLA", "."]} {"tags": [0, 0, 3, 0, 3, 4, 4, 4, 0, 0, 3, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["Effects", "of", "IL-4", "and", "Fc", "gamma", "receptor", "II", "engagement", "on", "Egr-1", "expression", "during", "stimulation", "of", "B", "lymphocytes", "by", "membrane", "immunoglobulin", "crosslinking", "."]} {"tags": [1, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Egr-1", "is", "an", "immediate", "early", "gene", "that", "is", "rapidly", "upregulated", "in", "response", "to", "mitogenic", "signals", "induced", "by", "antigen", "receptor", "crosslinking", "on", "murine", "B", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "has", "been", "shown", "that", "levels", "of", "Egr-1", "expression", "are", "closely", "correlated", "with", "B", "cell", "proliferation", "in", "several", "models", "of", "B", "cell", "activation", "and", "tolerance", "."]} {"tags": [0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 3, 4, 0], "tokens": ["We", "compared", "the", "expression", "of", "Egr-1", "during", "B", "cell", "stimulation", "with", "Fab'2", "and", "IgG", "anti-immunoglobulin", "(", "anti-Ig", ")", ",", "since", "it", "is", "known", "that", "Fab'2", "anti-Ig", "is", "mitogenic", "while", "IgG", "anti-Ig", "is", "not", ",", "owing", "to", "a", "dominant", "inhibitory", "effect", "of", "crosslinking", "the", "B", "cell", "Fc", "gamma", "RII", "to", "membrane", "Ig", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["While", "mitogenic", "doses", "of", "Fab'2", "anti-Ig", "induce", "large", "and", "rapid", "increases", "in", "Egr-1", "expression", ",", "IgG", "anti-Ig", "results", "in", "smaller", "increases", "in", "Egr-1", "mRNA", ",", "comparable", "to", "that", "seen", "with", "submitogenic", "concentrations", "of", "Fab'2", "anti-Ig", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 0], "tokens": ["However", ",", "the", "correlation", "between", "Egr-1", "expression", "and", "B", "cell", "proliferation", "breaks", "down", "when", "IL-4", "is", "added", "as", "a", "co-mitogen", "to", "induce", "B", "cell", "proliferation", "with", "IgG", "anti-Ig", "or", "submitogenic", "concentrations", "of", "Fab'2", "anti-Ig", "."]} {"tags": [0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 3, 0, 0, 0], "tokens": ["No", "corresponding", "increases", "in", "Egr-1", "mRNA", "levels", "are", "observed", "when", "IL-4", "is", "added", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0], "tokens": ["Therefore", ",", "IL-4", "overcomes", "Fc", "receptor-mediated", "inhibition", "of", "B", "cell", "proliferation", "without", "affecting", "inhibition", "of", "Egr-1", "mRNA", "induction", ",", "as", "demonstrated", "earlier", "for", "c-myc", "mRNA", "in", "this", "system", "."]} {"tags": [0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0], "tokens": ["Identification", "of", "a", "novel", "cyclosporin-sensitive", "element", "in", "the", "human", "tumor", "necrosis", "factor", "alpha", "gene", "promoter", "."]} {"tags": [3, 4, 4, 4, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Tumor", "necrosis", "factor", "alpha", "(", "TNF-alpha", ")", ",", "a", "cytokine", "with", "pleiotropic", "biological", "effects", ",", "is", "produced", "by", "a", "variety", "of", "cell", "types", "in", "response", "to", "induction", "by", "diverse", "stimuli", "."]} {"tags": [0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "paper", ",", "TNF-alpha", "mRNA", "is", "shown", "to", "be", "highly", "induced", "in", "a", "murine", "T", "cell", "clone", "by", "stimulation", "with", "T", "cell", "receptor", "(", "TCR", ")", "ligands", "or", "by", "calcium", "ionophores", "alone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Induction", "is", "rapid", ",", "does", "not", "require", "de", "novo", "protein", "synthesis", ",", "and", "is", "completely", "blocked", "by", "the", "immunosuppressant", "cyclosporin", "A", "(", "CsA", ")", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "identified", "a", "human", "TNF-alpha", "promoter", "element", ",", "kappa", "3", ",", "which", "plays", "a", "key", "role", "in", "the", "calcium-mediated", "inducibility", "and", "CsA", "sensitivity", "of", "the", "gene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["In", "electrophoretic", "mobility", "shift", "assays", ",", "an", "oligonucleotide", "containing", "kappa", "3", "forms", "two", "DNA", "protein", "complexes", "with", "proteins", "that", "are", "present", "in", "extracts", "from", "unstimulated", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "complexes", "appear", "in", "nuclear", "extracts", "only", "after", "T", "cell", "stimulation", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Induction", "of", "the", "inducible", "nuclear", "complexes", "is", "rapid", ",", "independent", "of", "protein", "synthesis", ",", "and", "blocked", "by", "CsA", ",", "and", "thus", ",", "exactly", "parallels", "the", "induction", "of", "TNF-alpha", "mRNA", "by", "TCR", "ligands", "or", "by", "calcium", "ionophore", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0], "tokens": ["Our", "studies", "indicate", "that", "the", "kappa", "3", "binding", "factor", "resembles", "the", "preexisting", "component", "of", "nuclear", "factor", "of", "activated", "T", "cells", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 5, 6, 6, 0], "tokens": ["Thus", ",", "the", "TNF-alpha", "gene", "is", "an", "immediate", "early", "gene", "in", "activated", "T", "cells", "and", "provides", "a", "new", "model", "system", "in", "which", "to", "study", "CsA-sensitive", "gene", "induction", "in", "activated", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 7, 8, 8, 8, 0, 0, 0, 5, 0, 5, 0], "tokens": ["Differences", "in", "expression", "of", "transcription", "factor", "AP-1", "in", "human", "promyelocytic", "HL-60", "cells", "during", "differentiation", "towards", "macrophages", "versus", "granulocytes", "."]} {"tags": [0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Commitment", "of", "HL-60", "cells", "to", "macrophage", "or", "granulocytic", "differentiation", "was", "achieved", "by", "incubation", "with", "4", "beta-phorbol", "12-myristate", "13-acetate", "(", "PMA", ")", "for", "30-60", "min", "or", "with", "dimethyl", "sulphoxide", "(", "DMSO", ")", "for", "24", "h", "respectively", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "commitment", "stage", "towards", "PMA-induced", "macrophage", "differentiation", "was", "associated", "with", "increases", "in", "jun", "B", "and", "c-fos", "mRNA", "levels", ",", "as", "well", "as", "with", "an", "increase", "in", "the", "binding", "activity", "of", "transcription", "factor", "AP-1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["Nevertheless", ",", "gel", "retardation", "analysis", "indicated", "that", "the", "AP-1", "activity", "detected", "in", "untreated", "cells", "was", "drastically", "reduced", "during", "the", "commitment", "stage", "of", "DMSO-induced", "HL-60", "differentiation", "towards", "granulocytes", "."]} {"tags": [0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["When", "HL-60", "cells", "were", "treated", "with", "sodium", "butyrate", ",", "which", "induced", "monocytic", "differentiation", ",", "a", "remarkable", "increase", "in", "AP-1", "binding", "activity", "was", "detected", "."]} {"tags": [0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Treatment", "of", "HL-60", "cells", "with", "1", "alpha", ",", "25-dihydroxyvitamin", "D3", ",", "another", "monocytic", "differentiation", "agent", ",", "induced", "a", "weak", ",", "but", "appreciable", ",", "increase", "in", "AP-1", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0], "tokens": ["Furthermore", ",", "addition", "of", "sodium", "butyrate", "or", "1", "alpha", ",", "25-dihydroxyvitamin", "D3", "to", "HL-60", "cells", "induced", "the", "expression", "of", "c-fos", ",", "c-jun", ",", "jun", "B", "and", "jun", "D", "proto-oncogenes", "."]} {"tags": [0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "when", "HL-60", "cells", "were", "treated", "with", "retinoic", "acid", ",", "a", "granulocytic", "differentiation", "inducer", ",", "no", "enhanced", "AP-1", "binding", "activity", "was", "observed", ",", "and", "only", "a", "weak", "increase", "in", "jun", "D", "mRNA", "level", "was", "detected", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["These", "data", "indicate", "that", "formation", "of", "AP-1", "is", "not", "required", "for", "the", "induction", "of", "HL-60", "differentiation", "towards", "granulocytes", ",", "whereas", "induction", "of", "monocytic", "differentiation", "is", "correlated", "with", "an", "increase", "in", "AP-1", "activity", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 5, 6, 6, 6], "tokens": ["The", "differential", "expression", "of", "AP-1", "activity", "may", "be", "critical", "in", "the", "differentiation", "of", "HL-60", "cells", "towards", "monocytic", "or", "granulocytic", "lineages"]} {"tags": [3, 4, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptors", "and", "sensitivity", "in", "leukemias", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0], "tokens": ["In", "an", "attempt", "to", "investigate", "the", "utility", "of", "glucocorticoid", "receptor", "determination", "to", "predict", "clinical", "responsiveness", "in", "human", "leukemias", "we", "have", "studied", "glucocorticoid", "receptors", "in", "the", "leukemic", "cells", "from", "46", "patients", "and", "in", "the", "lymphocytes", "from", "18", "normal", "donors", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "the", "normal", "lymphocytes", "there", "were", "3", ",", "875", "(", "Median", ")", "specific", "binding", "sites", "per", "cell", "."]} {"tags": [0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "blasts", "from", "17", "patients", "with", "ANLL", "had", "on", "average", "higher", "levels", "of", "binding", "sites", "per", "cell", "(", "Median", "=", "7", ",", "250", ",", "range", ":", "0", "to", "15", ",", "295", ")", "than", "the", "other", "leukemias", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Of", "the", "15", "patients", "with", "CLL", ",", "six", "had", "received", "glucocorticoid", "treatment", "for", "3", "to", "5", "years", "."]} {"tags": [0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Their", "lymphocytes", "had", "lower", "number", "of", "receptors", "(", "Median", "=", "2", ",", "000", ")", "than", "the", "other", "cases", "which", "were", "newly", "diagnosed", "(", "Median", "=", "4", ",", "500", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Four", "patients", "had", "ALL/AUL", ",", "three", "patients", "had", "blast", "crisis", "as", "terminal", "phase", "of", "CML", ",", "and", "seven", "had", "leukemic", "Non-Hodgkin", "lymphomas", "(", "Median", "=", "3", ",", "500", "sites/cell", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0], "tokens": ["In", "24", "patients", "we", "have", "also", "studied", "the", "in", "vitro", "sensitivity", "of", "the", "leukemic", "cells", "to", "dexamethasone", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0], "tokens": ["There", "was", "no", "marked", "correlation", "between", "glucocorticoid", "receptor", "levels", "and", "in", "vitro", "sensitivity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["An", "attempt", "to", "correlate", "receptor", "levels", "with", "clinical", "responsiveness", "demonstrated", "that", "glucocorticoid", "receptor", "determination", "might", "be", "of", "value", "in", "patients", "with", "lymphoid", "malignancies", "but", "probably", "not", "in", "patients", "with", "other", "leukemias", "."]} {"tags": [3, 4, 4, 4, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0], "tokens": ["'Activation-labile", "'", "glucocorticoid-receptor", "complexes", "of", "a", "steroid-resistant", "variant", "of", "CEM-C7", "human", "lymphoid", "cells", "."]} {"tags": [0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["For", "cytoplasmic", "glucocorticoid-receptor", "complexes", "to", "enter", "and", "accumulate", "in", "the", "nucleus", "a", "temperature-dependent", "event", ",", "'activation", "'", "is", "required", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Activation", "can", "be", "achieved", "in", "vitro", "by", "increased", "ionic", "strength", ",", "dilution", "or", "gel", "filtration", "and", "is", "manifested", "by", "an", "increased", "affinity", "of", "steroid-receptor", "complex", "for", "DNA", "and", "an", "altered", "elution", "profile", "from", "ion-exchange", "resins", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Munck", "and", "Foley", "have", "shown", "that", "activated", "complexes", "isolated", "from", "thymocytes", "elute", "from", "DEAE-cellulose", "in", "a", "manner", "identical", "to", "complexes", "activated", "in", "vitro", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 7, 0, 0, 7, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 3, 4, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "report", "here", "that", "DEAE-cellulose", "chromatography", "of", "steroid-receptor", "complexes", "from", "CEM-C7", ",", "a", "cloned", "human", "leukaemic", "T-cell", "line", "sensitive", "to", "the", "cytolytic", "action", "of", "glucocorticoids", ",", "and", "its", "steroid-resistant", "subclone", "4R4", "demonstrated", "that", "steroid", "receptors", "of", "clone", "4R4", "can", "not", "form", "stable", "activated", "complexes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 0, 0, 0, 7, 8, 8, 0], "tokens": ["This", "defines", "a", "new", "defect", "in", "receptor", "action", ",", "activation", "lability", "(", "r+act1", ")", ",", "which", "is", "unlike", "either", "the", "r-", ",", "r+nt-", ",", "or", "r+nti", "phenotypes", "previously", "described", "for", "mouse", "lymphoid", "variants", "."]} {"tags": [5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Granulocytes", "in", "the", "endometrium", "of", "post-partum", "women", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Endometrial", "samples", "of", "women", "at", "various", "stages", "of", "gonadal", "activity", "after", "parturition", "were", "examined", "for", "the", "presence", "and", "numbers", "of", "endometrial", "granulocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "samples", "at", "all", "the", "stages", "contained", "significant", "numbers", "of", "the", "granulocytes", "(", "i.e.", "greater", "than", "7/high-power", "field", ")", ",", "the", "100", "%", "values", "for", "late-proliferative", "and", "adaptation", "hyperplasia", "were", "significantly", "higher", "than", "the", "values", "for", "the", "resting", "(", "81.8", "%", ")", ",", "early", "(", "82.4", "%", ")", "and", "mid-", "(", "87.9", "%", ")", "proliferative", "and", "secretory", "(", "83.3", "%", ")", "phases", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "suggest", "that", "this", "correlates", "with", "the", "suggestion", "that", "the", "granulocytes", "constitute", "a", "receptor", "system", "for", "oestrogens", "."]} {"tags": [0, 0, 0, 0, 5, 0], "tokens": ["Interaction", "of", "glucocorticoids", "with", "macrophages", "."]} {"tags": [0, 0, 3, 4, 0, 5, 0, 5, 0], "tokens": ["Identification", "of", "glucocorticoid", "receptors", "in", "monocytes", "and", "macrophages", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 5, 6, 6, 0, 0, 5, 6, 0], "tokens": ["Glucocorticoid", "binding", "was", "measured", "in", "resident", "and", "thioglycollate-elicited", "mouse", "peritoneal", "macrophages", ",", "rabbit", "alveolar", "macrophages", ",", "and", "human", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Two", "assays", "of", "binding", "were", "used", "--", "an", "assay", "with", "intact", "cells", "in", "suspension", "or", "monolayers", ",", "and", "an", "assay", "of", "cytosol", "and", "nuclear", "forms", "of", "glucocorticoid", "receptors", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "mononuclear", "phagocytes", "contained", "approximately", "equal", "to", "4", "--", "10", "X", "10", "(", "3", ")", "high", "affinity", "receptor", "sites", "per", "cell", ",", "with", "dissociation", "constants", "of", "approximately", "equal", "to", "2", "--", "8", "nM", "dexamethasone", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "binding", "to", "the", "saturable", "sites", "was", "specific", "for", "steroids", "with", "glucocorticoid", "or", "antiglucocorticoid", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cortisol", ",", "corticosterone", ",", "and", "progesterone", "competed", "with", "dexamethasone", "for", "binding", ",", "whereas", "estradiol", ",", "dihydrotestosterone", ",", "and", "11-epicortisol", "competed", "very", "little", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Binding", "of", "dexamethasone", "to", "cytosol", "and", "nuclear", "forms", "of", "the", "receptor", "complex", "and", "temperature-sensitive", "translocation", "of", "cytosol", "forms", "to", "nuclear", "forms", "were", "shown", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["At", "37", "degrees", "C", "the", "predominant", "form", "of", "the", "hormone-receptor", "complex", "was", "nuclear", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0], "tokens": ["These", "results", "demonstrate", "that", "corticosteroids", "interact", "with", "macrophages", "at", "physiological", "concentrations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Nitric", "oxide", "signaling", ":", "a", "possible", "role", "for", "G", "proteins", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["We", "have", "previously", "reported", "various", "inductive", "effects", "of", "nitric", "oxide", "on", "human", "PBMC", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 0, 0], "tokens": ["We", "describe", "a", "novel", "and", "potentially", "important", "mechanism", "of", "nitric", "oxide", "signaling-through", "direct", "activation", "of", "guanine", "nucleotide-binding", "proteins", "(", "G", "proteins", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "found", "that", "nitric", "oxide", "treatment", "of", "membranes", "isolated", "from", "fresh", "human", "PBMC", "enhances", "the", "ability", "of", "these", "membranes", "to", "hydrolyze", "[", "gamma-32P", "]", "GTP", "and", "bind", "[", "gamma-35S", "]", "GTP", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "treatment", "of", "whole", "cells", "with", "nitric", "oxide", "yielded", "membranes", "with", "enhanced", "GTPase", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 4, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "the", "GTPase", "activity", "of", "pure", ",", "recombinant", "Gs", "alpha", ",", "Gi", "alpha", "1", ",", "and", "p21ras", "was", "greatly", "enhanced", "by", "nitric", "oxide", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["In", "support", "of", "the", "existence", "of", "this", "pathway", "in", "whole", "cells", ",", "we", "found", "that", "the", "G", "protein", "inhibitor", ",", "GDP-beta-S", ",", "blocked", "NF-kappa", "B", "translocation", "induced", "by", "nitric", "oxide", "or", "LPS", "in", "permeabilized", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "nitric", "oxide", "greatly", "reduced", "the", "pertussis", "toxin-mediated", "ADP-ribosylation", "of", "45-", "and", "41-kDa", "proteins", "in", "membranes", "of", "these", "cells", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Because", "G", "proteins", "play", "a", "central", "role", "in", "many", "diverse", "signaling", "systems", ",", "activation", "by", "an", "endogenous", "and", "inducible", "oxidant", "may", "represent", "a", "novel", "signaling", "pathway", "."]} {"tags": [0, 1, 2, 2, 2, 2, 2, 1, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["The", "granulocyte-macrophage", "colony-stimulating", "factor", "promoter", "cis-acting", "element", "CLE0", "mediates", "induction", "signals", "in", "T", "cells", "and", "is", "recognized", "by", "factors", "related", "to", "AP1", "and", "NFAT", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Expression", "of", "the", "granulocyte-macrophage", "colony-stimulating", "factor", "(", "GM-CSF", ")", "gene", "in", "T", "cells", "is", "activated", "by", "the", "combination", "of", "phorbol", "ester", "(", "phorbol", "myristate", "acetate", ")", "and", "calcium", "ionophore", "(", "A23187", ")", ",", "which", "mimic", "antigen", "stimulation", "through", "the", "T-cell", "receptor", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 0, 0, 7, 8, 8, 8, 0], "tokens": ["We", "have", "previously", "shown", "that", "a", "fragment", "containing", "bp", "-95", "to", "+27", "of", "the", "mouse", "GM-CSF", "promoter", "can", "confer", "inducibility", "to", "reporter", "genes", "in", "the", "human", "Jurkat", "T-cell", "line", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "use", "an", "in", "vitro", "transcription", "system", "to", "demonstrate", "that", "a", "cis-acting", "element", "(", "positions", "-54", "to", "-40", ")", ",", "referred", "to", "as", "CLE0", ",", "is", "a", "target", "for", "the", "induction", "signals", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["We", "observed", "induction", "with", "templates", "containing", "intact", "CLE0", "but", "not", "with", "templates", "with", "deleted", "or", "mutated", "CLE0", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "also", "observed", "that", "two", "distinct", "signals", "were", "required", "for", "the", "stimulation", "through", "CLE0", ",", "since", "only", "extracts", "from", "cells", "treated", "with", "both", "phorbol", "myristate", "acetate", "and", "A23187", "supported", "optimal", "induction", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["Stimulation", "probably", "was", "mediated", "by", "CLE0-binding", "proteins", "because", "depletion", "of", "these", "proteins", "specifically", "reduced", "GM-CSF", "transcription", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["One", "of", "the", "binding", "factors", "possessed", "biochemical", "and", "immunological", "features", "identical", "to", "those", "of", "the", "transcription", "factor", "AP1", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Another", "factor", "resembled", "the", "T-cell-specific", "factor", "NFAT", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "characteristics", "of", "these", "two", "factors", "are", "consistent", "with", "their", "involvement", "in", "GM-CSF", "induction", "."]} {"tags": [0, 0, 0, 1, 2, 0, 0, 1, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["The", "presence", "of", "CLE0-like", "elements", "in", "the", "promoters", "of", "interleukin-3", "(", "IL-3", ")", ",", "IL-4", ",", "IL-5", ",", "GM-CSF", ",", "and", "NFAT", "sites", "in", "the", "IL-2", "promoter", "suggests", "that", "the", "factors", "we", "detected", ",", "or", "related", "factors", "that", "recognize", "these", "sites", ",", "may", "account", "for", "the", "coordinate", "induction", "of", "these", "genes", "during", "T-cell", "activation", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 1, 0, 0, 1, 2, 2, 2, 0], "tokens": ["Identification", "and", "characterization", "of", "an", "Alu-containing", ",", "T-cell-specific", "enhancer", "located", "in", "the", "last", "intron", "of", "the", "human", "CD8", "alpha", "gene", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Expression", "of", "the", "human", "CD8", "alpha", "gene", "is", "restricted", "to", "cells", "of", "the", "lymphoid", "lineage", "and", "developmentally", "regulated", "during", "thymopoiesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["As", "an", "initial", "step", "towards", "understanding", "the", "molecular", "basis", "for", "tissue-specific", "expression", "of", "this", "gene", ",", "we", "surveyed", "the", "surrounding", "chromatin", "structure", "for", "potential", "cis-acting", "regulatory", "regions", "by", "DNase", "I", "hypersensitivity", "mapping", "and", "found", "four", "hypersensitive", "sites", ",", "three", "of", "which", "were", "T", "cell", "restricted", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["By", "using", "a", "reporter-based", "expression", "approach", ",", "a", "T-cell-specific", "enhancer", "was", "identified", "by", "its", "close", "association", "with", "a", "prominent", "T-cell-restricted", "hypersensitive", "sites", "in", "the", "last", "intron", "of", "the", "CD8", "alpha", "gene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Deletion", "studies", "demonstrated", "that", "the", "minimal", "enhancer", "is", "adjacent", "to", "a", "negative", "regulatory", "element", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 1, 2, 2, 0, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["DNA", "sequence", "analysis", "of", "the", "minimal", "enhancer", "revealed", "a", "striking", "cluster", "of", "consensus", "binding", "sites", "for", "Ets-1", ",", "TCF-1", ",", "CRE", ",", "GATA-3", ",", "LyF-1", ",", "and", "bHLH", "proteins", "which", "were", "verified", "by", "electrophoretic", "mobility", "shift", "assays", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0], "tokens": ["In", "addition", ",", "the", "5", "'", "end", "of", "the", "enhancer", "was", "composed", "of", "an", "Alu", "repeat", "which", "contained", "the", "GATA-3", ",", "bHLH", ",", "and", "LyF-1", "binding", "sites", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0], "tokens": ["Site-directed", "mutation", "of", "the", "Ets-1", "and", "GATA-3", "sites", "dramatically", "reduced", "enhancer", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "functional", "importance", "of", "the", "other", "binding", "sites", "only", "became", "apparent", "when", "combinations", "of", "mutations", "were", "analyzed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0], "tokens": ["Taken", "together", ",", "these", "results", "suggest", "that", "the", "human", "CD8", "alpha", "gene", "is", "regulated", "by", "the", "interaction", "of", "multiple", "T-cell", "nuclear", "proteins", "with", "a", "transcriptional", "enhancer", "located", "in", "the", "last", "intron", "of", "the", "gene", "."]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 1, 2, 0], "tokens": ["Comparison", "of", "the", "CD8", "alpha", "enhancer", "with", "other", "recently", "identified", "T-cell-specific", "regulatory", "elements", "suggests", "that", "a", "common", "set", "of", "transcription", "factors", "regulates", "several", "T-cell", "genes", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0], "tokens": ["Molecular", "regulation", "of", "the", "human", "IL-3", "gene", ":", "inducible", "T", "cell-restricted", "expression", "requires", "intact", "AP-1", "and", "Elf-1", "nuclear", "protein", "binding", "sites", "."]} {"tags": [3, 4, 0, 3, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Interleukin", "3", "(", "IL-3", ")", "is", "a", "hematopoietic", "stem-cell", "growth", "and", "differentiation", "factor", "that", "is", "expressed", "solely", "in", "activated", "T", "and", "NK", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Studies", "to", "date", "have", "identified", "elements", "5", "'", "to", "the", "IL-3", "coding", "sequences", "that", "regulate", "its", "transcription", ",", "but", "the", "sequences", "that", "confer", "T", "cell-specific", "expression", "remain", "to", "be", "clearly", "defined", "."]} {"tags": [0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0], "tokens": ["We", "have", "now", "identified", "DNA", "sequences", "that", "are", "required", "for", "T", "cell-restricted", "IL-3", "gene", "transcription", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 5, 6, 6, 6, 0, 0, 0, 1, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 5, 6, 0], "tokens": ["A", "series", "of", "transient", "transfections", "performed", "with", "human", "IL-3-chloramphenicol", "acetyltransferase", "(", "CAT", ")", "reporter", "plasmids", "in", "T", "and", "non-T", "cells", "revealed", "that", "a", "plasmid", "containing", "319", "bp", "of", "5", "'", "flanking", "sequences", "was", "active", "exclusively", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 2, 2, 0, 0, 0, 0, 1, 2, 2, 0, 3, 4, 4, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 0], "tokens": ["Deletion", "analysis", "revealed", "that", "T", "cell", "specificity", "was", "conferred", "by", "a", "49-bp", "fragment", "(", "bp", "-319", "to", "-270", ")", "that", "included", "a", "potential", "binding", "site", "for", "AP-1", "transcription", "factors", "6", "bp", "upstream", "of", "a", "binding", "site", "for", "Elf-1", ",", "a", "member", "of", "the", "Ets", "family", "of", "transcription", "factors", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 2, 0, 0, 1, 2, 2, 2, 2, 2, 0], "tokens": ["DNaseI", "footprint", "and", "electrophoretic", "mobility", "shift", "assay", "analyses", "performed", "with", "MLA-144", "T", "cell", "nuclear", "extracts", "demonstrated", "that", "this", "49-bp", "region", "contains", "a", "nuclear", "protein", "binding", "region", "that", "includes", "consensus", "AP-1", "and", "Elf-1", "binding", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0], "tokens": ["In", "addition", ",", "extracts", "prepared", "from", "purified", "human", "T", "cells", "contained", "proteins", "that", "bound", "to", "synthetic", "oligonucleotides", "corresponding", "to", "the", "AP-1", "and", "Elf-1", "binding", "sites", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0], "tokens": ["In", "vitro-transcribed", "and", "-translated", "Elf-1", "protein", "bound", "specifically", "to", "the", "Elf-1", "site", ",", "and", "Elf-1", "antisera", "competed", "and", "super", "shifted", "nuclear", "protein", "complexes", "present", "in", "MLA-144", "nuclear", "extracts", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Moreover", ",", "addition", "of", "anti-Jun", "family", "antiserum", "in", "electrophoretic", "mobility", "shift", "assay", "reactions", "completely", "blocked", "formation", "of", "the", "AP-1-related", "complexes", "."]} {"tags": [0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 0, 1, 2, 2, 2, 0, 1, 2, 2, 2, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Transient", "transfection", "studies", "in", "MLA-144", "T", "cells", "revealed", "that", "constructs", "containing", "mutations", "in", "the", "AP-1", "site", "almost", "completely", "abolished", "CAT", "activity", "while", "mutation", "of", "the", "Elf-1", "site", "or", "the", "NF-IL-3", "site", ",", "a", "previously", "described", "nuclear", "protein", "binding", "site", "(", "bp.", "-155", "to", "-148", ")", "in", "the", "IL-3", "promoter", ",", "reduced", "CAT", "activity", "to", "<", "25", "%", "of", "the", "activity", "given", "by", "wild-type", "constructs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 3, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["We", "conclude", "that", "expression", "of", "the", "human", "IL-3", "gene", "requires", "the", "AP-1", "and", "Elf-1", "binding", "sites", ";", "however", ",", "unlike", "other", "previously", "characterized", "cytokine", "genes", "such", "as", "IL-2", ",", "the", "AP-1", "and", "Elf-1", "factors", "can", "bind", "independently", "in", "the", "IL-3", "gene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["(", "ABSTRACT", "TRUNCATED", "AT", "400", "WORDS", ")"]} {"tags": [0, 3, 0, 3, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0], "tokens": ["Combination", "IL-2", "and", "IL-4", "reduces", "glucocorticoid", "receptor-binding", "affinity", "and", "T", "cell", "response", "to", "glucocorticoids", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "mechanisms", "contributing", "to", "persistent", "T", "cell", "activation", "and", "poor", "response", "to", "glucocorticoids", "in", "chronic", "inflammatory", "illnesses", "such", "as", "steroid", "resistant", "(", "SR", ")", "asthma", "are", "poorly", "defined", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "examined", "the", "possibility", "that", "certain", "cytokines", ",", "specifically", "IL-2", "and", "IL-4", ",", "could", "affect", "T", "cell", "response", "to", "glucocorticoids", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["A", "[", "3H", "]", "dexamethasone", "radioligand-binding", "assay", "was", "used", "to", "measure", "the", "number", "of", "glucocorticoid", "receptors", "(", "GR", ")", "and", "dissociation", "constant", "(", "Kd", ")", "in", "PBMC", "from", "normal", "donors", "and", "patients", "with", "SR", "asthma", ",", "cultured", "in", "the", "absence", "and", "presence", "of", "these", "cytokines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["PBMC", "from", "normal", "donors", "incubated", "for", "48", "h", "in", "the", "presence", "of", "combination", "IL-2", "+", "IL-4", "had", "nuclear", "GR", "with", "significantly", "reduced", "binding", "affinity", "(", "GR", "Kd", "=", "36.1", "+/-", "1.63", "nM", ",", "mean", "+/-", "SEM", ";", "p", "=", "0.0001", ")", "as", "compared", "with", "PBMC", "incubated", "with", "medium", "alone", "(", "GR", "Kd", "=", "6.74", "+/-", "0.46", "nM", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "cytosolic", "GR", "Kd", "remained", "unchanged", "."]} {"tags": [0, 0, 0, 5, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "when", "PBMC", "were", "incubated", "with", "IL-2", "alone", "or", "IL-4", "alone", ",", "no", "change", "in", "GR-binding", "affinity", "was", "observed", "."]} {"tags": [0, 0, 0, 5, 6, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "when", "T", "cells", "and", "non-T", "cells", "were", "individually", "stimulated", "with", "combination", "IL-2", "+", "IL-4", ",", "a", "significant", "reduction", "in", "GR-binding", "affinity", "was", "observed", "only", "in", "the", "T", "cell", "population", "(", "p", "=", "0.0001", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "IL-2", "+", "IL-4-induced", "alteration", "in", "PBMC", "GR", "Kd", "was", "associated", "with", "an", "increase", "in", "GR", "number", "(", "8348", "+/-", "964", "vs", "1710", "+/-", "228", "sites/cell", ";", "p", "=", "0.0003", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0], "tokens": ["More", "importantly", ",", "the", "alteration", "in", "PBMC", "GR-binding", "affinity", "with", "IL-2", "+", "IL-4", "was", "associated", "with", "a", "functional", "change", "in", "T", "cell", "response", "to", "methylprednisolone", "MPN", ",", "i.e.", ",", "a", "reduced", "inhibitory", "effect", "of", "MPN", "on", "PMA/ionomycin-induced", "T", "cell", "proliferation", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 5, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0], "tokens": ["These", "effects", "of", "IL-2", "+", "IL-4", "on", "PBMC", "GR", "affinity", "and", "response", "to", "MPN", "were", "blocked", "by", "co-incubation", "with", "IFN-gamma", "."]} {"tags": [0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Freshly", "isolated", "PBMC", "from", "four", "patients", "with", "SR", "asthma", "had", "a", "significantly", "reduced", "GR-binding", "affinity", "(", "Kd", "=", "40.0", "+/-", "2.68", "nM", ";", "p", "=", "0.0001", ")", "when", "compared", "with", "seven", "normal", "subjects", "(", "7.15", "+/-", "0.41", "nM", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["The", "altered", "PBMC", "GR", "binding", "from", "patients", "with", "SR", "asthma", "reversed", "to", "normal", "when", "incubated", "with", "medium", "alone", ",", "but", "was", "sustained", "with", "IL-2", "+", "IL-4", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "observations", "suggest", "that", "with", "persistent", "inflammation", "certain", "cytokines", "may", "contribute", "to", "an", "impaired", "response", "to", "glucocorticoids", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 0], "tokens": ["Furthermore", ",", "the", "effects", "of", "IL-2", "and", "IL-4", "were", "blocked", "by", "IFN-gamma", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Characterization", "of", "the", "human", "CD4", "gene", "promoter", ":", "transcription", "from", "the", "CD4", "gene", "core", "promoter", "is", "tissue-specific", "and", "is", "activated", "by", "Ets", "proteins", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 1, 2, 2, 0], "tokens": ["We", "analyzed", "the", "5", "'", "transcription", "control", "sequences", "of", "the", "human", "CD4", "gene", "."]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 2, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["We", "located", "the", "transcription", "initiation", "site", "and", "showed", "that", "the", "CD4", "core", "promoter", "(", "positions", "-40", "to", "+16", ")", "lacks", "a", "classical", "``", "TATA", "''", "or", "initiator", "positioning", "consensus", "sequence", "but", "directs", "precise", "and", "efficient", "transcription", "when", "coupled", "to", "the", "ubiquitously", "active", "simian", "virus", "40", "enhancer", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "transcriptional", "activity", "of", "the", "CD4", "gene", "promoter", "correlated", "with", "CD4", "expression", "in", "various", "cell", "types", "."]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interestingly", ",", "the", "CD4", "core", "promoter", "also", "displayed", "a", "tissue-specific", "transcriptional", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0], "tokens": ["Within", "this", "fragment", ",", "three", "nucleic", "acid", "sequences", "are", "completely", "conserved", "in", "the", "murine", "CD4", "gene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["One", "of", "these", "sequences", "contains", "a", "perfect", "ETS", "consensus", "sequence", "."]} {"tags": [0, 1, 2, 2, 0, 0, 0, 0, 0, 0], "tokens": ["Another", "ETS", "consensus", "sequence", "is", "located", "1060", "nt", "upstream", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Electrophoretic-mobility-shift", "assays", "showed", "that", "the", "core", "promoter", "ETS", "motif", "binds", "an", "Ets-related", "protein", "specifically", "expressed", "at", "high", "levels", "in", "CD4+", "cells", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 0], "tokens": ["Moreover", ",", "in", "CD4-", "cells", ",", "overexpression", "of", "Ets-1", "or", "Ets-2", "efficiently", "and", "specifically", "activated", "transcription", "from", "the", "CD4", "promoter", "and", "core", "promoter", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 1, 2, 2], "tokens": ["These", "data", "indicate", "that", "Ets", "transcription", "factors", "play", "a", "central", "role", "in", "controlling", "CD4", "gene", "expression", ",", "by", "binding", "to", "both", "a", "classical", "remote", "site", "and", "an", "unusual", "proximal", "activator", "sequence"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Glucocorticoid", "receptor", "activation", "and", "inactivation", "in", "c", "ultured", "human", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", "glucocorticoids", "are", "not", "cytolytic", "for", "and", "do", "not", "inhibit", "the", "growth", "of", "the", "IM-9", "line", "of", "cultured", "human", "lymphoblasts", ",", "these", "cells", "have", "a", "high", "steroid-binding", "capacity", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["We", "have", "used", "IM-9", "cells", "in", "order", "to", "examine", "whether", "unoccupied", "glucocorticoid", "receptors", "are", "inactivated", "and", "activated", "in", "intact", "cells", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["when", "IM-9", "cells", "are", "incubated", "in", "glucose-free", "medium", "in", "a", "nitrogen", "atmosphere", ",", "both", "their", "ability", "to", "bind", "triamcinolone", "acetonide", "and", "their", "ATP", "levels", "decline", "and", ",", "when", "glucose", "and", "oxygen", "are", "reintroduced", ",", "ATP", "levels", "and", "receptor", "activity", "return", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "specific", "glucocorticoid-binding", "activity", "of", "cytosol", "prepared", "from", "cells", "exposed", "to", "various", "degrees", "of", "energy", "limitation", "is", "directly", "correlated", "with", "the", "ATP", "content", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Receptor", "activation", "in", "intact", "cells", "is", "rapid", "and", "independent", "of", "protein", "synthesis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cytosol", "prepared", "from", "inactivated", "cells", "can", "not", "be", "activated", "by", "addition", "of", "ATP", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "inactivation", "of", "glucocorticoid", "receptors", "that", "occurs", "when", "cytosol", "from", "normal", "IM-9", "cells", "is", "incubated", "at", "25", "degrees", "C", "is", "inhibited", "by", "molybdate", ",", "vanadate", ",", "fluoride", ",", "ATP", ",", "and", "several", "other", "nucleotides", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["The", "experiments", "with", "intact", "human", "lymphoblasts", "suggest", "that", "assays", "of", "specific", "glucocorticoid-binding", "capacity", "do", "not", "necessarily", "reflect", "the", "cellular", "content", "of", "receptor", "protein", "."]} {"tags": [0, 0, 5, 0], "tokens": ["Glucocorticoids", "and", "lymphocytes", "."]} {"tags": [0, 0], "tokens": ["II", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0], "tokens": ["Cell", "cycle-dependent", "changes", "in", "glucocorticoid", "receptor", "content", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "study", "variations", "in", "glucocorticoid", "receptor", "levels", "during", "the", "cell", "cycle", ",", "we", "have", "separated", "mitogen-stimulated", "human", "peripheral", "lymphocytes", "and", "rat", "lymph", "node", "cells", "by", "unit", "gravity", "sedimentation", "and", "measured", "glucocorticoid", "binding", "in", "the", "resultant", "fractions", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 0], "tokens": ["By", "morphologic", "criteria", "and", "thymidine", "incorporation", ",", "the", "fractions", "were", "separated", "into", "populations", "of", "G0", "and", "G1", "phase", "and", "S", "and", "post-S", "phase", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 7, 8, 8, 8, 8, 0], "tokens": ["A", "2-", "to", "3-fold", "increase", "in", "glucocorticoid", "receptor", "sites", "per", "cell", ",", "for", "cells", "in", "the", "S", "and", "post-S", "phase", "over", "those", "in", "G0", "and", "G1", ",", "was", "observed", "with", "both", "nonstimulated", "rat", "lymph", "node", "cell", "suspensions", "and", "concanavalin", "A-stimulated", "human", "peripheral", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["These", "observations", "together", "with", "those", "from", "other", "studies", "indicate", "that", "formation", "of", "new", "glucocorticoid", "receptors", "near", "the", "S", "phase", "may", "be", "a", "general", "phenomenon", "in", "proliferating", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 0], "tokens": ["We", "propose", "that", "this", "increase", "in", "glucocorticoid", "receptors", "during", "the", "cell", "cycle", "may", "explain", "the", "increase", "in", "glucocorticoid", "receptors", "in", "mitogen-stimulated", "lymphocytes", "."]} {"tags": [0, 5, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "leukocyte", "migration", "inhibition", "response", "to", "certain", "breast", "cancer-related", "antigens", "(", "MCF-7", "and", "MuMTV", ")", ":", "their", "potential", "as", "discriminants", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Certain", "oncogenic", "viruses", "have", "been", "implicated", "in", "human", "breast", "cancer", ",", "including", "the", "murine", "mammary", "tumor", "virus", "(", "MuMTV", ")", "and", "the", "Mason-Pfizer", "monkey", "virus", "(", "MPMV", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 7, 8, 8, 8, 8, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "used", "the", "leukocyte", "migration", "inhibition", "(", "LMI", ")", "response", "to", "assay", "the", "response", "to", "several", "potential", "breast", "cancer-related", "antigens", ",", "including", "MuMTV", ",", "MPMV", ",", "and", "a", "breast", "cancer", "cultured", "cell", "line", ",", "MCF-7", ",", "in", "96", "breast", "cancer", "patients", ",", "in", "32", "women", "with", "benign", "breast", "disease", ",", "and", "in", "67", "normal", "women", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "lowest", "tenth", "percentile", "of", "control", "(", "LMI", ")", "responses", "was", "used", "as", "the", "cutoff", "point", "to", "designate", "responders", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["Breast", "cancer", "patients", "showed", "significant", "responses", "to", "MuMTV", "(", "49", "%", "and", "to", "MCF-7", "(", "50", "%", ")", ",", "but", "not", "to", "MPMV", "(", "29", "%", ")", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "a", "paired-antigen", "study", "using", "MuMTV", "and", "MCF-7", ",", "75", "%", "of", "the", "breast", "cancer", "patients", "responded", ",", "versus", "18", "%", "of", "the", "normal", "women", "(", "P", "less", "than", "0.0050", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "potential", "for", "this", "assay", "to", "distinguish", "``", "normal", "''", "from", "``", "breast", "cancer", "''", "was", "analyzed", "using", "a", "migration", "index", "derived", "from", "discriminant", "analysis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "ability", "of", "the", "assay", "to", "discriminate", "``", "normal", "''", "from", "``", "cancer", "''", "was", "significant", "(", "P", "less", "than", "0.001", ")", "and", "showed", "a", "sensitivity", "of", "detecting", "``", "cancer", "''", "of", "75", "%", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "overall", "responses", "to", "MuMTV", "and", "MCF-7", "were", "analyzed", "with", "reference", "to", "certain", "prognostic", "factors", ",", "but", "showed", "no", "relation", "to", "age", ",", "menstrual", "status", ",", "estrogen", "receptor", "status", ",", "or", "stage", "of", "disease", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "above", "reactions", "suggest", "that", "a", "large", "proportion", "of", "breast", "cancer", "patients", "exhibit", "presensitization", "to", "antigenfs", "found", "in", "MuMTV", "and", "MCF-7", ",", "which", "may", "be", "cross-reactive", "with", "antigens", "in", "the", "primary", "cancer", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "responses", "appear", "to", "be", "independent", "of", "major", "prognostic", "variables", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Further", "refinement", "of", "this", "assay", "may", "yield", "one", "which", "is", "more", "highly", "discriminating", "for", "breast", "cancer", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Chronic", "lymphatic", "leukaemia", ":", "cellular", "effects", "of", "glucocorticoids", "in", "vitro", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoid", "receptor", "levels", "and", "steroid", "induced", "inhibition", "of", "nucleic", "acid", "precursors", "have", "been", "examined", "in", "lymphocytes", "from", "27", "patients", "at", "different", "stages", "of", "chronic", "lymphatic", "leukaemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["No", "correlation", "can", "be", "found", "between", "the", "level", "of", "glucocorticoid", "receptors", "and", "the", "stage", "of", "the", "disease", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["On", "the", "other", "hand", ",", "a", "significant", "difference", "(", "P", "less", "than", "0.02", ")", "was", "found", "between", "stage", "O", "and", "stage", "III/IV", "patients", ",", "in", "terms", "of", "the", "in", "vitro", "effect", "of", "dexamethasone", "on", "[", "3H", "]", "uridine", "incorporation", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Pyrrolidine", "dithiocarbamate", "inhibits", "NF-kappa", "B", "mobilization", "and", "TNF", "production", "in", "human", "monocytes", "."]} {"tags": [0, 1, 2, 2, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "human", "TNF", "promoter", "contains", "four", "potential", "nuclear", "factor-kappa", "B", "(", "NF-kappa", "B", ")", "-binding", "sites", ",", "with", "the", "strongest", "binding", "seen", "for", "the", "-605", "motif", "."]} {"tags": [0, 0, 0, 7, 8, 0, 0, 7, 8, 8, 8, 0, 7, 8, 8, 0, 0, 0, 3, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Nuclear", "extracts", "from", "unstimulated", "cells", "of", "the", "human", "monocytic", "cell", "line", ",", "Mono", "Mac", "6", ",", "contain", "one", "specific", "binding", "protein", "(", "complex", "II", ")", ",", "consistent", "with", "a", "constitutive", "p50", "homodimer", "."]} {"tags": [0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Stimulation", "of", "Mono", "Mac", "6", "cells", "with", "LPS", "will", "increase", "complex", "II", "and", "will", "strongly", "induce", "a", "second", "specific", "complex", "(", "complex", "I", ")", ",", "which", "represents", "the", "p50/65", "heterodimer", "."]} {"tags": [0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0], "tokens": ["Treatment", "of", "Mono", "Mac", "6", "cells", "with", "pyrrolidine-dithiocarbamate", "(", "PDTC", ")", "at", "300", "microM", "will", "block", "the", "LPS-induced", "complex", "I", "almost", "completely", "and", "will", "reduce", "complex", "II", "to", "the", "constitutive", "level", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Binding", "activity", "of", "other", "nuclear", "factors", "that", "recognize", "the", "SP-1", "and", "c/EBP", "motifs", "of", "the", "human", "TNF", "promoter", "is", "not", "affected", "by", "such", "treatment", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Northern", "blot", "analysis", "demonstrates", "that", "PDTC", "treatment", "will", "strongly", "reduce", "LPS-induced", "TNF", "transcripts", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Secreted", "TNF", "protein", "as", "detected", "in", "the", "Wehi", "164S/ActD", "bioassay", "and", "in", "a", "sandwich", "immunoassay", "was", "similarly", "reduced", "by", "PDTC", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["Kinetic", "analyses", "show", "that", "after", "LPS", "stimulation", ",", "NF-kappa", "B", "will", "peak", "at", "1", "h", ",", "TNF", "transcript", "prevalence", "at", "2", "h", ",", "and", "TNF", "protein", "at", "4", "h", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["PDTC", "did", "not", "shift", "this", "response", "to", "LPS", "to", "a", "later", "time", ",", "but", "suppressed", "NF-kappa", "B", "mobilization", ",", "TNF", "transcripts", ",", "and", "TNF", "protein", "over", "the", "entire", "8-h", "observation", "period", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Analysis", "of", "freshly", "isolated", ",", "LPS-stimulated", "blood", "monocytes", "showed", "a", "similar", "blockade", "of", "NF-kappa", "B", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0], "tokens": ["Furthermore", ",", "in", "these", "primary", "cells", ",", "induction", "of", "TNF", "transcripts", ",", "as", "determined", "by", "Northern", "blot", "analysis", "and", "by", "quantitative", "polymerase", "chain", "reaction", ",", "was", "prevented", "by", "PDTC", "as", "was", "TNF", "protein", "production", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["These", "data", "show", "that", "dithiocarbamates", "can", "profoundly", "affect", "cytokine", "expression", "and", "suggest", "that", "NF-kappa", "B", "is", "involved", "in", "LPS-induced", "TNF", "gene", "expression", "in", "human", "monocytes", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Comparing", "regions", "of", "the", "Epstein-Barr", "virus", "ZEBRA", "protein", "which", "function", "as", "transcriptional", "activating", "sequences", "in", "Saccharomyces", "cerevisiae", "and", "in", "B", "cells", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 1, 2, 0, 5, 6, 6, 0], "tokens": ["The", "ZEBRA", "protein", "activates", "expression", "of", "Epstein-Barr", "virus", "early-lytic-cycle", "genes", "in", "human", "B", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["Here", "it", "is", "shown", "that", "ZEBRA", "also", "behaves", "as", "a", "sequence-specific", "transcriptional", "activator", "in", "Saccharomyces", "cerevisiae", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Deletional", "mutagenesis", "defined", "three", "regions", "of", "ZEBRA", "that", "participate", "in", "activation", "in", "S.", "cerevisiae", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 3, 0, 3, 4, 4, 4, 0, 0, 0, 3, 0, 3, 4, 4, 4, 0, 0], "tokens": ["These", "regions", "are", "designated", "YI", "(", "amino", "acids", "[", "aa", "]", "1", "to", "25", ")", ",", "YII", "(", "aa", "51", "to", "102", ")", ",", "and", "YIII", "(", "aa", "228", "to", "245", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Two", "of", "the", "three", "regions", "of", "the", "native", "ZEBRA", "protein", "act", "together", "to", "mediate", "activation", "when", "assayed", "on", "ZEBRA", "binding", "sites", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "when", "fused", "to", "the", "DNA", "binding", "domain", "of", "GAL", "4", "and", "assayed", "on", "GAL4", "binding", "sites", ",", "regions", "YII", "and", "YIII", "were", "each", "sufficient", "to", "confer", "activation", "in", "S.", "cerevisiae", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Regions", "of", "ZEBRA", "which", "affected", "activation", "in", "S.", "cerevisiae", "were", "also", "required", "in", "human", "B", "lymphocytes", "."]} {"tags": [0, 3, 4, 0, 3, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "amino-terminal", "region", "of", "ZEBRA", "(", "aa", "1", "to", "98", ")", "was", "required", "for", "activation", "both", "in", "S.", "cerevisiae", "and", "in", "human", "B", "cells", ";", "deletion", "of", "the", "carboxy-terminal", "18", "aa", "also", "significantly", "reduced", "activation", "in", "both", "cell", "types", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Thus", ",", "the", "behavior", "of", "ZEBRA", "in", "human", "B", "cells", "and", "S.", "cerevisiae", "suggests", "that", "the", "protein", "contains", "universal", "activation", "motifs", "that", "interact", "with", "conserved", "components", "of", "the", "transcription", "machinery", "."]} {"tags": [0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["However", ",", "certain", "deletion", "mutants", "of", "ZEBRA", "containing", "mutations", "in", "the", "N-terminal", "region", "exhibited", "discordant", "behaviors", "in", "S.", "cerevisiae", "and", "in", "B", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["For", "example", ",", "deletion", "of", "ZEBRA", "aa", "26", "to", "51", "impaired", "activation", "to", "a", "great", "extent", "in", "B", "cells", "but", "had", "little", "or", "no", "effect", "in", "S.", "cerevisiae", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "discordant", "mutants", "may", "reflect", "interactions", "with", "a", "variable", "domain", "of", "a", "conserved", "component", "or", "unique", "interactions", "with", "specialized", "components", "of", "the", "basal", "transcription", "apparatus", "in", "different", "cells", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 3, 4, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Functional", "interaction", "of", "the", "v-Rel", "and", "c-Rel", "oncoproteins", "with", "the", "TATA-binding", "protein", "and", "association", "with", "transcription", "factor", "IIB", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Rel", "family", "proteins", "regulate", "the", "expression", "of", "genes", "linked", "to", "kappa", "B-binding", "motifs", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Little", "is", "known", ",", "however", ",", "of", "the", "mechanism", "by", "which", "they", "enhance", "transcription", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "investigated", "the", "ability", "of", "the", "v-Rel", "and", "c-Rel", "oncoproteins", "to", "interact", "with", "components", "of", "the", "basal", "transcription", "machinery", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 4, 0, 3, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", "we", "report", "that", "both", "the", "acidic", "transcription", "activation", "domain", "mapping", "to", "the", "unique", "C", "terminus", "of", "chicken", "c-Rel", "and", "the", "F9", "cell-specific", "activation", "region", "common", "to", "both", "v-Rel", "and", "c-Rel", "interact", "with", "the", "TATA-binding", "protein", "(", "TBP", ")", "and", "transcription", "factor", "IIB", "(", "TFIIB", ")", "in", "vitro", "and", "in", "vivo", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0], "tokens": ["We", "also", "demonstrate", "that", "TPB", "interaction", "with", "Rel", "activation", "regions", "leads", "to", "synergistic", "activation", "of", "transcription", "of", "a", "kappa", "B-linked", "reporter", "gene", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Combined", "with", "the", "observation", "that", "the", "mouse", "c-Rel", "and", "human", "RelA", "proteins", "also", "interact", "with", "TBP", "and", "TFIIB", "in", "vitro", ",", "these", "results", "suggest", "that", "association", "with", "basal", "transcription", "factors", "is", "important", "for", "the", "transcriptional", "activities", "of", "Rel", "family", "proteins", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 0], "tokens": ["p21ras", "and", "calcineurin", "synergize", "to", "regulate", "the", "nuclear", "factor", "of", "activated", "T", "cells", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 5, 6, 0, 7, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 3, 4, 0], "tokens": ["In", "T", "lymphocytes", ",", "triggering", "of", "the", "T", "cell", "receptor", "(", "TCR", ")", "induces", "several", "signaling", "cascades", "which", "ultimately", "synergize", "to", "induce", "the", "activity", "of", "the", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NFAT", ")", ",", "a", "DNA", "binding", "complex", "critical", "to", "the", "inducibility", "and", "T", "cell", "specificity", "of", "the", "T", "cell", "growth", "factor", "interleukin", "2", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["One", "immediate", "consequence", "of", "T", "cell", "activation", "via", "the", "TCR", "is", "an", "increase", "in", "cytosolic", "calcium", "."]} {"tags": [0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Calcium", "signals", "are", "important", "for", "NFAT", "induction", ",", "and", "recent", "studies", "have", "identified", "calcineurin", ",", "a", "calcium-calmodulin", "dependent", "serine-threonine", "phosphatase", ",", "as", "a", "prominent", "component", "of", "the", "calcium", "signaling", "pathway", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 4, 0, 0, 0], "tokens": ["A", "second", "important", "molecule", "in", "TCR", "signal", "transduction", "is", "the", "guanine", "nucleotide", "binding", "protein", ",", "p21ras", ",", "which", "is", "coupled", "to", "the", "TCR", "by", "a", "protein", "tyrosine", "kinase", "dependent", "mechanism", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 5, 0, 0], "tokens": ["The", "experiments", "presented", "here", "show", "that", "expression", "by", "transfection", "of", "mutationally", "activated", "calcineurin", "or", "activated", "p21ras", "alone", "is", "insufficient", "for", "NFAT", "transactivation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0], "tokens": ["However", ",", "coexpression", "of", "the", "activated", "calcineurin", "with", "activated", "p21ras", "could", "mimic", "TCR", "signals", "in", "NFAT", "induction", "."]} {"tags": [0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 5, 6, 0, 0], "tokens": ["These", "data", "identify", "calcineurin", "and", "p21ras", "as", "cooperative", "partners", "in", "T", "cell", "activation", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Expression", "of", "the", "chicken", "GATA", "factor", "family", "during", "early", "erythroid", "development", "and", "differentiation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 0, 1, 2, 0, 0, 3, 4, 0, 0, 0, 0, 1, 2, 0, 0, 3, 4, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["The", "DNA", "motif", "WGATAR", "has", "been", "identified", "within", "transcriptional", "regulatory", "domains", "of", "globin", "and", "other", "erythroid-specific", "genes", "and", "the", "activator", "proteins", "that", "bind", "to", "this", "regulatory", "element", ",", "the", "GATA", "factors", ",", "belong", "to", "a", "multi-gene", "family", "that", "is", "expressed", "in", "chicken", "erythroid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["Here", "we", "show", "that", ",", "as", "in", "chickens", ",", "multiple", "members", "of", "the", "GATA", "factor", "family", "are", "expressed", "in", "human", "and", "murine", "erythroid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["During", "the", "early", "stages", "of", "chicken", "embryogenesis", "(", "well", "before", "blood", "island", "formation", ")", ",", "each", "of", "the", "GATA", "family", "members", "is", "transcribed", "with", "a", "unique", "temporal", "and", "spatial", "pattern", "."]} {"tags": [0, 0, 5, 6, 6, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0], "tokens": ["In", "the", "primitive", "erythroid", "lineage", ",", "transcription", "of", "the", "embryonic", "epsilon-globin", "gene", "parallels", "GATA-1", "expression", "while", "the", "switch", "to", "beta-globin", "transcription", "in", "definitive", "erythroid", "cells", "is", "directly", "preceded", "by", "a", "pronounced", "increase", "in", "GATA-3", "accumulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "timing", "and", "pattern", "of", "expression", "of", "these", "different", "mRNAs", "during", "avian", "erythroid", "development", "and", "differentiation", "suggests", "that", "temporally", "regulated", "changes", "in", "GATA", "factor", "expression", "are", "required", "for", "vertebrate", "hematopoiesis", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 5, 0, 0, 3, 4, 0], "tokens": ["Molecular", "regulation", "of", "human", "interleukin", "2", "and", "T-cell", "function", "by", "interleukin", "4", "."]} {"tags": [0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Distinct", "functional", "T-cell", "subsets", ",", "differing", "in", "the", "patterns", "of", "lymphokines", "produced", ",", "regulate", "cell-mediated", "and", "humoral", "immune", "responses", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 4, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "two", "major", "types", "and", "their", "principal", "products", ",", "interleukin", "4", "and", "interferon", "gamma", "(", "IL-4", "and", "IFN-gamma", ")", ",", "are", "reciprocally", "negatively", "interactive", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 3, 0], "tokens": ["To", "analyze", "the", "molecular", "mechanism", "of", "IL-4-mediated", "suppression", "of", "cell-mediated", "immunity", "we", "studied", "its", "effects", "on", "expression", "of", "interleukin", "2", "(", "IL-2", ")", "and", "IFN-gamma", "."]} {"tags": [3, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["IL-4", "pretreatment", "of", "Jurkat", "cells", "prior", "to", "stimulation", "resulted", "in", "a", "decrease", "in", "transcription", "of", "the", "IL2", "gene", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["IL-4", "suppressed", "IL-2", "and", "IFN-gamma", "mRNA", "levels", "in", "primary", "human", "T", "cells", ",", "and", "addition", "of", "anti-CD28", "antibodies", "relieved", "this", "suppression", "."]} {"tags": [0, 1, 2, 0, 3, 0, 0, 0, 1, 2, 0], "tokens": ["Using", "enhancer-reporter", "constructs", ",", "IL-4", "specifically", "down-regulated", "the", "NFIL-2B", "element", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 3, 0, 0, 3, 4, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 0, 0], "tokens": ["Electrophoretic", "mobility", "shift", "assays", "using", "a", "DNA", "oligomer", "containing", "the", "NFIL-2B", "binding", "site", "indicated", "that", "IL-4", "inhibited", "the", "NFIL-2B", "complex", "and", "that", "the", "NFIL-2B", "DNA", "binding", "factor", "is", "distinct", "from", "AP-", "1", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 1, 2], "tokens": ["These", "results", "suggest", "that", "IL-4", "may", "regulate", "development", "and", "function", "of", "T-cell", "subsets", "involved", "in", "cell-mediated", "immunity", "in", "part", "by", "inhibiting", "factors", "required", "for", "transcription", "of", "the", "IL2", "gene"]} {"tags": [0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 6, 0], "tokens": ["Immunochemical", "differences", "between", "glucocorticoid", "receptors", "from", "corticoid-sensitive", "and", "-resistant", "malignant", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 6, 0], "tokens": ["We", "have", "explored", "the", "possibility", "of", "using", "antibodies", "against", "purified", "rat", "liver", "glucocorticoid", "receptors", "to", "study", "the", "immunochemical", "properties", "of", "glucocorticoid", "receptors", "from", "murine", "and", "human", "malignant", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 3, 4, 0], "tokens": ["For", "this", "purpose", ",", "purified", "immune", "immunoglobulin", "G", "was", "covalently", "linked", "to", "Sepharose", "CL-4B", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 8, 8, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 5, 6, 6, 0, 0, 0, 0, 7, 0, 0], "tokens": ["We", "then", "examined", "the", "ability", "of", "the", "affinity", "gel", "to", "recognize", "cytosolic", "[", "3H", "]", "triamcinolone", "acetonide-receptor", "complexes", "from", "the", "corticoid-sensitive", "(", "CS", ")", "and", "-resistant", "strains", "of", "mouse", "lymphoma", "P1798", ",", "from", "CS", "lymphocytes", "of", "patients", "with", "chronic", "lymphatic", "leukemia", ",", "and", "from", "a", "CS", "clone", "of", "human", "leukemic", "lymphoblasts", "in", "tissue", "culture", "(", "CH6", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0], "tokens": ["Mouse", "thymus", "was", "used", "as", "a", "source", "of", "glucocorticoid", "receptor", "from", "normal", "CS", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0, 0, 0, 7, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 7, 8, 8, 8, 0], "tokens": ["Whereas", "the", "immunoaffinity", "column", "retained", "70", "to", "84", "%", "of", "the", "58-", "to", "62-A", "(", "Stokes", "radius", ")", "[", "3H", "]", "triamcinolone", "acetonide-receptor", "complexes", "characteristic", "of", "the", "CS", "mouse", "and", "human", "lymphocytes", ",", "it", "failed", "to", "recognize", "the", "27-", "to", "28-A", "(", "Stokes", "radius", ")", "glucocorticoid", "receptor", "present", "in", "corticoid-resistant", "mouse", "lymphoma", "P1798", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Therefore", ",", "under", "appropriate", "experimental", "conditions", ",", "it", "was", "possible", "to", "demonstrate", "cross-reactivity", "between", "the", "antiserum", "against", "rat", "liver", "glucocorticoid", "receptor", "and", "the", "58-", "to", "62-A", "(", "Stokes", "radius", ")", "glucocorticoid", "receptor", "from", "species", "as", "diverse", "as", "mouse", "and", "humans", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Heterogeneity", "of", "the", "in", "vitro", "responses", "to", "glucocorticoids", "in", "acute", "leukemia", "."]} {"tags": [0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "leukocyte", "population", "freshly", "isolated", "from", "the", "blood", "of", "26", "patients", "with", "acute", "leukemia", ",", "we", "have", "measured", "several", "parameters", "including", "glucocorticoid", "receptors", ",", "nucleoside", "incorporation", ",", "percentage", "of", "cells", "in", "S", "phase", ",", "and", "steroid-induced", "cell", "lysis", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "in", "some", "cases", ",", "the", "short-term", "response", "to", "steroid", "therapy", "was", "determined", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Although", ",", "in", "all", "the", "patients", "studied", ",", "leukocytes", "were", "found", "to", "contain", "glucocorticoid", "receptors", ",", "we", "failed", "to", "demonstrate", "any", "correlation", "between", "the", "level", "of", "binding", "sites", "and", "the", "in", "vitro", "or", "in", "vivo", "response", "to", "glucocorticoids", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["This", "absence", "of", "correlation", "could", "be", "in", "part", "explained", "by", "the", "marked", "heterogeneity", "of", "the", "steroid", "response", "demonstrated", "in", "leukocyte", "subpopulations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "appears", ",", "however", ",", "that", "the", "degree", "of", "steroid", "action", "in", "vitro", "as", "well", "as", "the", "extent", "of", "spontaneous", "and", "dexamethasone-induced", "cell", "death", "may", "be", "related", "to", "the", "number", "of", "cells", "in", "the", "S", "phase", "of", "the", "cell", "cycle", "."]} {"tags": [3, 4, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Glucocorticoid", "receptors", "in", "cytosol", "and", "nuclear", "extract", "of", "human", "leukocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cortisol", "binding", "by", "cytosol", "and", "0.4", "M", "KCl", "extract", "of", "the", "nuclear", "fraction", "of", "human", "leukocytes", "were", "studied", "by", "gel", "chromatography", "and", "ion", "exchange", "filtration", "on", "DEAE", "cellulose", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0], "tokens": ["The", "cytoplasmic", "cortisol", "binding", "protein", "has", "a", "molecular", "weight", "95", "000", "and", "the", "soluble", "nuclear", "binding", "protein", "50", "000", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "absence", "of", "the", "uptake", "of", "radioactive", "cortisol", "by", "isolated", "nuclei", "and", "the", "apparent", "requirement", "of", "the", "cytosol", "for", "glucocorticoid", "specific", "binding", "in", "nuclear", "receptor", "sites", "was", "observed", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "association", "constant", "characterising", "the", "binding", "of", "cortisol", "to", "cytosol", "was", "KA", "=", "3.5", ".", "10", "(", "9", ")", "l/mol", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Corticosteroid-induced", "lymphopenia", ",", "immunosuppression", ",", "and", "body", "defense", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0], "tokens": ["The", "apparent", "paradox", "of", "heightened", "adrenal", "corticosteroid", "levels", "associated", "with", "reduction", "in", "the", "competence", "of", "the", "body", "'s", "defensive", "apparatus", "to", "cope", "with", "exposure", "to", "new", "microbial", "antigens", "is", "considered", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "question", "is", "asked", "how", "this", "lowered", "defensive", "capability", ",", "which", "occurs", "in", "the", "face", "of", "a", "threat", "to", "body", "integrity", ",", "is", "consistent", "with", "Cannon", "'s", "principals", "of", "the", "``", "wisdom", "of", "the", "body.", "''"]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "suggestion", "is", "offered", "that", "the", "immunologic", "response", "to", "self-antigens", "exposed", "by", "disease", "or", "trauma", "may", "be", "suppressed", "by", "corticosteroid", "to", "offset", "the", "likelihood", "of", "autoimmune", "attack", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 5, 6, 6, 0], "tokens": ["Protein", "tyrosine", "kinase", "activation", "is", "required", "for", "lipopolysaccharide", "induction", "of", "cytokines", "in", "human", "blood", "monocytes", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 3, 0, 0, 3, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Bacterial", "LPS", "induce", "production", "of", "cytokines", "such", "as", "IL-1", ",", "IL-6", ",", "and", "TNF", "in", "mononuclear", "phagocytes", ",", "and", "this", "represents", "a", "central", "component", "in", "the", "pathogenesis", "of", "septic", "shock", "syndrome", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0], "tokens": ["However", ",", "the", "mechanisms", "by", "which", "LPS", "activates", "these", "cells", "to", "express", "cytokines", "are", "not", "completely", "characterized", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 0], "tokens": ["The", "present", "study", "addressed", "the", "role", "of", "different", "protein", "kinases", "in", "the", "LPS", "induction", "of", "cytokines", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "is", "shown", "that", "LPS", "induced", "a", "12-", "to", "16-fold", "increase", "in", "IL-1", "beta", ",", "IL-6", ",", "and", "TNF-alpha", "mRNA", "levels", ",", "and", "this", "was", "completely", "or", "more", "than", "80", "%", "blocked", "by", "the", "protein", "tyrosine", "kinase", "specific", "inhibitors", "herbimycin", "A", "and", "genistein", "at", "the", "concentrations", "of", "1.7", "and", "37", "microM", ",", "respectively", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Protein", "kinase", "C", "inhibition", "by", "staurosporine", "reduced", "LPS", "induction", "of", "TNF-alpha", ",", "whereas", "it", "had", "no", "effects", "on", "IL-6", "and", "IL-1", "beta", "."]} {"tags": [0, 0, 3, 4, 4, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "protein", "kinase", "A", "by", "H89", "reduced", "IL-6", "mRNA", "levels", "but", "did", "not", "detectably", "change", "IL-1", "beta", "or", "TNF-alpha", "mRNA", "levels", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "contrast", ",", "LPS", "did", "not", "increase", "leukemia", "inhibitory", "factor", "mRNA", ",", "which", "was", "constitutively", "expressed", "and", "not", "significantly", "reduced", "by", "these", "inhibitors", "."]} {"tags": [0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", "to", "cytokine", "mRNA", "levels", ",", "LPS-induced", "IL-6", "protein", "synthesis", "and", "IL-6", "bioactivity", "were", "also", "reduced", "to", "baseline", "levels", "by", "the", "PTK", "inhibitors", "herbimycin", "A", "and", "genistein", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 9, 10, 0, 0, 1, 0, 1, 0], "tokens": ["Both", "PTK", "inhibitors", "also", "reduced", "the", "LPS", "activation", "of", "nuclear", "factor-kappa", "B", "(", "NF-kappa", "B", ")", ",", "which", "is", "a", "transcription", "factor", "involved", "in", "the", "expression", "of", "cytokine", "genes", "such", "as", "IL-6", "and", "TNF-alpha", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "activation", "of", "NF-kappa", "B", "was", "also", "reduced", "by", "H89", ",", "whereas", "staurosporine", "had", "no", "effect", "on", "this", "response", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 5, 0], "tokens": ["In", "summary", ",", "these", "findings", "suggest", "that", "protein", "kinase", "C", "and", "protein", "kinase", "A", "appear", "to", "have", "selective", "effects", "in", "the", "LPS", "induction", "of", "cytokines", ",", "whereas", "PTK", "is", "required", "for", "LPS", "induction", "of", "a", "broad", "spectrum", "of", "cytokines", "and", "NF-kappa", "B", "activation", "in", "monocytes", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 4, 0], "tokens": ["In", "vivo", "control", "of", "NF-kappa", "B", "activation", "by", "I", "kappa", "B", "alpha", "."]} {"tags": [0, 3, 4, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 0], "tokens": ["The", "transcription", "factor", "NF-kappa", "B", "is", "stored", "in", "the", "cytoplasm", "in", "complexes", "with", "the", "inhibitor", "protein", "I", "kappa", "B", "alpha", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["It", "has", "been", "shown", "in", "vitro", "that", "dissociation", "of", "I", "kappa", "B", "alpha", "from", "these", "complexes", "results", "in", "active", "NF-kappa", "B", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 4, 0, 0, 0], "tokens": ["In", "this", "report", "we", "show", "that", "lipopolysaccharide", "(", "LPS", ")", "-induced", "activation", "of", "B", "or", "pre-B", "cells", "results", "in", "loss", "of", "I", "kappa", "B", "alpha", "from", "NF-kappa", "B", "complexes", "in", "vivo", "."]} {"tags": [0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Many", "liberated", "NF-kappa", "B", "dimers", "reached", "the", "nucleus", ",", "where", "increased", "c-rel", ",", "p65", "and", "p50", "were", "detected", "by", "immunoblotting", "and", "by", "DNA", "binding", "assays", "."]} {"tags": [0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Some", "liberated", "dimers", "were", "retained", "in", "the", "cytoplasm", ",", "however", ",", "through", "binding", "to", "newly", "synthesized", "I", "kappa", "B", "alpha", ",", "a", "finding", "which", "strongly", "suggests", "(", "i", ")", "that", "the", "LPS-induced", "signal", "causes", "dissociation", "of", "complexes", "rather", "than", "preventing", "their", "association", "and", "(", "ii", ")", "that", "dissociation", "results", "from", "modification", "of", "I", "kappa", "B", "alpha", "and", "not", "of", "c-rel", "or", "p65", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0], "tokens": ["No", "effect", "of", "LPS", "treatment", "was", "detected", "on", "p105", "or", "p100", ",", "which", "also", "retain", "rel", "family", "members", "in", "the", "cytoplasm", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["Quite", "unexpectedly", ",", "we", "also", "found", "that", "in", "unstimulated", "cells", "there", "is", "a", "constant", "ongoing", "process", "of", "degradation", "and", "replacement", "of", "complexed", "I", "kappa", "B", "alpha", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "propose", "that", "this", "turnover", "results", "in", "the", "low", "level", "of", "active", "NF-kappa", "B", "presumably", "necessary", "even", "in", "the", "unstimulated", "cell", ",", "and", "that", "the", "high", "rate", "of", "synthesis", "of", "I", "kappa", "B", "alpha", "provides", "the", "ability", "to", "turn", "off", "NF-kappa", "B", "activity", "rapidly", "as", "soon", "as", "the", "activating", "signal", "ceases", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Identification", "of", "a", "killer", "cell-specific", "regulatory", "element", "of", "the", "mouse", "perforin", "gene", ":", "an", "Ets-binding", "site-homologous", "motif", "that", "interacts", "with", "Ets-related", "proteins", "."]} {"tags": [0, 0, 0, 0, 3, 4, 3, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["The", "gene", "encoding", "the", "cytolytic", "protein", "perforin", "is", "selectively", "expressed", "by", "activated", "killer", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 0, 1, 0, 0], "tokens": ["To", "understand", "the", "mechanisms", "underlying", "the", "cell-type-specific", "expression", "of", "this", "gene", ",", "we", "have", "characterized", "the", "regulatory", "functions", "and", "the", "DNA-protein", "interactions", "of", "the", "5'-flanking", "region", "of", "the", "mouse", "perforin", "gene", "(", "Pfp", ")", "."]} {"tags": [0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "region", "extending", "from", "residues", "+62", "through", "-141", ",", "which", "possesses", "the", "essential", "promoter", "activity", ",", "and", "regions", "further", "upstream", ",", "which", "are", "able", "to", "either", "enhance", "or", "suppress", "gene", "expression", ",", "were", "identified", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "region", "between", "residues", "-411", "and", "-566", "was", "chosen", "for", "further", "characterization", ",", "since", "it", "contains", "an", "enhancer-like", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "identified", "a", "32-mer", "sequence", "(", "residues", "-491", "to", "-522", ")", "which", "appeared", "to", "be", "capable", "of", "enhancing", "gene", "expression", "in", "a", "killer", "cell-specific", "manner", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0], "tokens": ["Within", "this", "segment", ",", "a", "9-mer", "motif", "(", "5'-ACAGGAAGT-3", "'", ",", "residues", "-505", "to", "-497", ";", "designated", "NF-P", "motif", ")", ",", "which", "is", "highly", "homologous", "to", "the", "Ets", "proto-oncoprotein-binding", "site", ",", "was", "found", "to", "interact", "with", "two", "proteins", ",", "NF-P1", "and", "NF-P2", "."]} {"tags": [3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["NF-P2", "appears", "to", "be", "induced", "by", "reagents", "known", "to", "up-regulate", "the", "perforin", "message", "level", "and", "is", "present", "exclusively", "in", "killer", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 4, 0], "tokens": ["Electrophoretic", "mobility", "shift", "assay", "and", "UV", "cross-linking", "experiments", "revealed", "that", "NF-P1", "and", "NF-P2", "may", "possess", "common", "DNA-binding", "subunits", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["However", ",", "the", "larger", "native", "molecular", "mass", "of", "NF-P1", "suggests", "that", "NF-P1", "contains", "an", "additional", "non-DNA-binding", "subunit", "(", "s", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 3, 4, 4, 0], "tokens": ["In", "view", "of", "the", "homology", "between", "the", "NF-P", "motif", "and", "other", "Ets", "proto-oncoprotein-binding", "sites", ",", "it", "is", "postulated", "that", "NF-P1", "and", "NF-P2", "belong", "to", "the", "Ets", "protein", "family", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Results", "obtained", "from", "the", "binding", "competition", "assay", ",", "nevertheless", ",", "suggest", "that", "NF-P1", "and", "NF-P2", "are", "related", "to", "but", "distinct", "from", "Ets", "proteins", ",", "e.g.", ",", "Ets-1", ",", "Ets-2", ",", "and", "NF-AT/Elf-1", ",", "known", "to", "be", "expressed", "in", "T", "cells", "."]} {"tags": [0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 5, 6, 6, 0, 3, 4, 4, 4, 4, 0], "tokens": ["The", "role", "of", "NF-kappa", "B1", "(", "p50/p105", ")", "gene", "expression", "in", "activation", "of", "human", "blood", "T-lymphocytes", "via", "CD2", "and", "CD28", "adhesion", "molecules", "."]} {"tags": [0, 0, 5, 6, 6, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Stimulation", "of", "primary", "human", "T-lymphocytes", "via", "CD2", "and", "CD28", "adhesion", "molecules", "induces", "a", "long-lasting", "proliferation", "(", ">", "3", "weeks", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0], "tokens": ["This", "potent", "activation", "does", "not", "require", "accessory", "cells", ",", "such", "as", "monocytes", ",", "but", "depends", "on", "persistent", "interleukin", "2", "(", "IL-2", ")", "secretion", "and", "receptivity", ",", "which", "is", "associated", "with", "high", "and", "prolonged", "expression", "of", "the", "inducible", "CD25/IL-2", "receptor", "alpha", "(", "IL-2R", "alpha", ")", "chain", "gene", "."]} {"tags": [0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0], "tokens": ["The", "transcription", "factor", "NF-kappa", "B", "participates", "in", "the", "regulation", "of", "both", "IL-2", "and", "IL-2R", "alpha", "genes", ",", "as", "well", "as", "multiple", "cellular", "genes", "involved", "in", "T-cell", "proliferation", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0], "tokens": ["To", "evaluate", "the", "role", "of", "NF-kappa", "B", "in", "human", "peripheral", "blood", "T-lymphocytes", ",", "we", "previously", "analyzed", "the", "activation", "of", "NF-kappa", "B-related", "complexes", "in", "response", "to", "CD2+CD28", "costimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4], "tokens": ["We", "demonstrated", "a", "long-term", "induction", "of", "p50/p65", "heterodimer", ",", "a", "putative", "p65/c-Rel", "heterodimer", ",", "and", "a", "constitutive", "nuclear", "expression", "of", "KBF1/p50", "homodimers", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0], "tokens": ["As", "the", "role", "of", "p50", "remains", "unclear", ",", "we", "focused", "our", "present", "study", "on", "NF-kappa", "B1", "(", "p50/p105", ")", "gene", "regulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 3, 0], "tokens": ["Using", "electrophoretic", "mobility", "shift", "assays", "and", "Western", "and", "Northern", "blot", "analyses", ",", "we", "studied", "NF-kappa", "B1", "gene", "expression", "during", "T-cell", "stimulation", "via", "CD2+CD28", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "observed", "a", "transient", "4-", "to", "5-fold", "increase", "of", "NF-kappa", "B1", "gene", "expression", "at", "both", "the", "mRNA", "and", "protein", "levels", ",", "lasting", "for", "at", "least", "24", "h", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0], "tokens": ["p50", "DNA-binding", "activity", "apparently", "stays", "highly", "controlled", "when", "p105", "expression", "is", "enhanced", "by", "a", "physiological", "stimulus", "of", "peripheral", "blood", "T-cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["Partial", "inhibition", "of", "p50", "and", "p105", "expression", "by", "NF-kappa", "B1", "antisense", "oligonucleotides", "significantly", "reduced", "T-cell", "proliferation", "and", "CD25/IL-2R", "alpha", "cell", "surface", "expression", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0], "tokens": ["(", "ABSTRACT", "TRUNCATED", "AT", "250", "WORDS", ")"]} {"tags": [3, 4, 4, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0], "tokens": ["Vitamin", "D", "receptor", "quantitation", "in", "human", "blood", "mononuclear", "cells", "in", "health", "and", "disease", "."]} {"tags": [3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Vitamin", "D", "receptor", "(", "VDR", ")", "concentration", "was", "quantitated", "in", "human", "peripheral", "blood", "mononuclear", "cells", "(", "PBMC", ")", "from", "patients", "with", "absorptive", "hypercalciuria", "(", "AH", ")", "and", "patients", "with", "high", "1", ",", "25", "(", "OH", ")", "2D3", "due", "to", "acquired", "or", "transient", "disease", "states", "and", "the", "results", "compared", "to", "those", "in", "normal", "subjects", "."]} {"tags": [0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0], "tokens": ["VDR", "concentration", "in", "resting", "cells", "was", "not", "different", "between", "the", "three", "groups", "and", "represented", "constitutive", "receptor", "expression", "of", "monocytes", "."]} {"tags": [0, 0, 0, 3, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Following", "activation", "with", "phytohemagglutinin", ",", "patients", "with", "hypercalcitriolemia", "demonstrated", "significantly", "greater", "VDR", "concentrations", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Patients", "with", "AH", "demonstrated", "a", "normal", "value", "for", "the", "group", ",", "but", "6", "patients", "had", "significantly", "greater", "concentrations", "of", "VDR", "despite", "normal", "plasma", "1", ",", "25", "(", "OH", ")", "2D3", "in", "four", "of", "the", "patients", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Proliferation", ",", "as", "assessed", "from", "[", "3H", "]", "thymidine", "incorporation", "was", "inversely", "correlated", "with", "serum", "1", ",", "25", "(", "OH", ")", "2D3", "and", "was", "significant", "(", "R", "=", "-0.299", ",", "p", "=", "0.048", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Taken", "together", ",", "the", "results", "suggest", "that", "PBMC", "provide", "a", "useful", "system", "for", "studying", "VDR", "status", "in", "transient", "or", "acquired", "states", "of", "hypercalcitriolemia", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Furthermore", ",", "the", "studies", "in", "patients", "with", "absorptive", "hypercalciuria", "disclosed", "it", "to", "be", "a", "heterogeneous", "disorder", ",", "characterized", "by", "both", "vitamin", "D-dependent", "and", "D-independent", "forms", "of", "receptor", "up-regulation", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 5, 6, 0], "tokens": ["Reactive", "oxygen", "intermediates", "activate", "NF-kappa", "B", "in", "a", "tyrosine", "kinase-dependent", "mechanism", "and", "in", "combination", "with", "vanadate", "activate", "the", "p56lck", "and", "p59fyn", "tyrosine", "kinases", "in", "human", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "have", "previously", "observed", "that", "ionizing", "radiation", "induces", "tyrosine", "phosphorylation", "in", "human", "B-lymphocyte", "precursors", "by", "stimulation", "of", "unidentified", "tyrosine", "kinases", "and", "this", "phosphorylation", "is", "substantially", "augmented", "by", "vanadate", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Ionizing", "radiation", "generates", "reactive", "oxygen", "intermediates", "(", "ROI", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Because", "H2O2", "is", "a", "potent", "ROI", "generator", "that", "readily", "crosses", "the", "plasma", "membrane", ",", "we", "used", "H2O2", "to", "examine", "the", "effects", "of", "ROI", "on", "signal", "transduction", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "now", "provide", "evidence", "that", "the", "tyrosine", "kinase", "inhibitor", "herbimycin", "A", "and", "the", "free", "radical", "scavenger", "N-acetyl-cysteine", "inhibit", "both", "radiation-induced", "and", "H2O2-induced", "activation", "of", "NF-kappa", "B", ",", "indicating", "that", "activation", "triggered", "by", "ROI", "is", "dependent", "on", "tyrosine", "kinase", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["H2O2", "was", "found", "to", "stimulate", "Ins-1", ",", "4", ",", "5-P3", "production", "in", "a", "tyrosine", "kinase-dependent", "manner", "and", "to", "induce", "calcium", "signals", "that", "were", "greatly", "augmented", "by", "vanadate", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["The", "synergistic", "induction", "of", "tyrosine", "phosphorylation", "by", "H2O2", "plus", "vanadate", "included", "physiologically", "relevant", "proteins", "such", "as", "PLC", "gamma", "1", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["Although", "treatment", "of", "cells", "with", "H2O2", "alone", "did", "not", "affect", "the", "activity", "of", "src", "family", "kinases", ",", "treatment", "with", "H2O2", "plus", "vanadate", "led", "to", "activation", "of", "the", "p56lck", "and", "p59fyn", "tyrosine", "kinases", "."]} {"tags": [0, 0, 0, 0, 3, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "combined", "inhibition", "of", "phosphatases", "and", "activation", "of", "kinases", "provides", "a", "potent", "mechanism", "for", "the", "synergistic", "effects", "of", "H2O2", "plus", "vanadate", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4], "tokens": ["Induction", "of", "tyrosine", "phosphorylation", "by", "ROI", "may", "thus", "lead", "to", "many", "of", "the", "pleiotropic", "effects", "of", "ROI", "in", "lymphoid", "cells", ",", "including", "downstream", "activation", "of", "PLC", "gamma", "1", "and", "NF-kappa", "B"]} {"tags": [0, 0, 3, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Influence", "of", "sex", "hormone", "binding", "globulin", "and", "serum", "albumin", "on", "the", "conversion", "of", "androstenedione", "to", "testosterone", "by", "human", "erythrocytes", "."]} {"tags": [0, 0, 0, 3, 4, 4, 0, 3, 4, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0], "tokens": ["The", "influence", "of", "human", "serum", "albumin", "and", "sex", "hormone", "binding", "globulin", "(", "SHBG", ")", "on", "the", "enzymic", "conversion", "of", "androstenedione", "to", "testosterone", "in", "human", "erythrocytes", "was", "investigated", "in", "vitro", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0], "tokens": ["Total", "plasma", "and", "albumin", "delayed", "the", "conversion", "rate", "of", "androstenedione", ",", "while", "SHBG", "increased", "it", "markedly", "."]} {"tags": [0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "effect", "of", "SHBG", "was", "largely", "abolished", "by", "heating", "to", "60", "degrees", "C", "for", "1", "h", "and", "by", "saturating", "its", "binding", "sites", "by", "DHT", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "effect", "of", "both", "proteins", "was", "found", "to", "be", "related", "to", "their", "concentration", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["It", "appears", "that", "the", "binding", "sites", "of", "albumin", "provide", "a", "mechanism", "for", "retarding", "androstenedione", "uptake", "by", "the", "erythrocytes", "and", "that", "the", "high", "binding", "affinity", "of", "SHBG", "for", "testosterone", "facilitates", "the", "diffusion", "of", "this", "steroid", "out", "of", "the", "cell", "and", "thus", ",", "displaces", "the", "chemical", "equilibrium", "within", "the", "cell", "."]} {"tags": [0, 0, 5, 0], "tokens": ["Glucocorticoids", "and", "lymphocytes", "."]} {"tags": [0, 0], "tokens": ["I", "."]} {"tags": [0, 3, 4, 0, 0, 7, 8, 0], "tokens": ["Increased", "glucocorticoid", "receptor", "levels", "in", "antigen-stimulated", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Recently", "a", "2-", "to", "3-fold", "increase", "in", "the", "number", "of", "glucocorticoid", "receptors", "in", "human", "peripheral", "lymphocytes", "has", "been", "noted", "after", "in", "vitro", "mitogen", "stimulation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Here", ",", "we", "extend", "these", "observations", "to", "in", "vivo", "immunization", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["After", "unilateral", "immunization", "of", "adrenalectomized", "male", "rats", ",", "a", "50", "%", "increase", "in", "glucocorticoid", "receptor", "sites", "per", "cell", ",", "determined", "by", "binding", "of", "dexamethasone", ",", "was", "observed", "in", "cell", "suspensions", "of", "homolateral", "lymph", "nodes", "over", "those", "from", "the", "contralateral", "nonimmunized", "side", "of", "the", "same", "animal", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "association", "constant", "for", "dexamethasone", "was", "similar", "in", "both", "groups", ",", "as", "was", "the", "stereospecificity", "for", "various", "steroids", ",", "the", "time", "course", "of", "cytoplasmic", "and", "nuclear", "association", ",", "and", "cytoplasmic-to-nuclear", "translocation", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Despite", "a", "50", "%", "increase", "in", "the", "number", "of", "glucocorticoid", "receptor", "sites", "per", "cell", ",", "the", "cells", "from", "the", "homolateral", "and", "controlateral", "lymph", "nodes", "were", "equally", "sensitive", "to", "the", "inhibitory", "effects", "of", "dexamethasone", ",", "as", "determined", "by", "measurements", "of", "the", "incorporation", "of", "radiolabeled", "precursors", "of", "protein", ",", "RNA", ",", "and", "DNA", ",", "or", "measurements", "of", "in", "vitro", "cell", "survival", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 5, 6, 6, 0], "tokens": ["Glucocorticoid", "receptors", "and", "actions", "in", "rat", "thymocytes", "and", "immunologically", "stimulated", "human", "peripheral", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0], "tokens": ["After", "reviewing", "briefly", "our", "earlier", "studies", "on", "glucocorticoid", "receptors", "and", "mechanisms", "in", "thymus", "cells", ",", "we", "have", "outlined", "results", "from", "the", "following", "two", "areas", "of", "current", "interest", "in", "our", "laboratories", ":", "the", "``", "life-cycle", "''", "of", "glucocorticoid", "receptors", "and", "complexes", "in", "thymus", "cells", ",", "and", "the", "levels", "of", "glucocorticoid", "receptors", "and", "sensitivity", "in", "immunologically", "stimulated", "human", "peripheral", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Several", "of", "our", "results", "on", "energetics", "and", "kinetics", "of", "hormone", "binding", "to", "glucocorticoid", "receptors", "in", "rat", "thymus", "cells", "seem", "to", "require", "extension", "of", "the", "simplest", "model", "of", "hormone-receptor", "transformations", "in", "intact", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["ATP-depletion", "experiments", "suggest", "the", "existence", "of", "a", "nonbinding", "form", "of", "the", "receptor", ";", "``", "chase", "''", "experiments", "suggest", "reaction", "of", "hormone", "directly", "with", "nuclear-bound", "receptor", ";", "experiments", "on", "depletion", "and", "replenishment", "of", "cytoplasmic", "receptor", "using", "cortisol", "and", "dexamethasone", "suggest", "the", "existence", "of", "at", "least", "two", "subpopulations", "of", "nuclear-bound", "hormone-receptor", "complex", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0], "tokens": ["We", "have", "found", "that", "mitogen", "or", "immunologic", "stimulation", "of", "human", "peripheral", "lymphocytes", "in", "culture", "leads", "within", "24", "h", "or", "so", "to", "a", "striking", "increase", "in", "the", "number", "of", "glucocorticoid", "receptor", "sites", "per", "cell", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["We", "believe", "this", "increase", "may", "be", "due", "to", "partial", "synchronization", "of", "the", "cell", "population", "in", "a", "phase", "of", "the", "cell", "cycle", "in", "which", "receptor", "content", "is", "high", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Contrary", "to", "the", "widely", "held", "view", "that", "mitogen-stimulated", "cells", "become", "insensitive", "to", "glucocorticoids", ",", "our", "experiments", "show", "that", "with", "respect", "to", "inhibition", "of", "thymidine", "and", "uridine", "incorporation", "and", "glucose", "uptake", ",", "the", "cells", "are", "highly", "sensitive", "to", "dexamethasone", "at", "24", ",", "48", ",", "and", "72", "h", "after", "stimulation", "with", "concanavalin", "A", "."]} {"tags": [0, 0, 0, 0, 5, 6, 6, 6, 0], "tokens": ["Nuclear", "glucocorticoid", "binding", "in", "chronic", "lymphatic", "leukemia", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["A", "reliable", "procedure", "is", "described", "for", "isolating", "3H-triamcinolone", "acetonide", "(", "3H-TA", ")", "receptor", "complexes", "from", "purified", "chronic", "lymphatic", "leukemia", "(", "CLL", ")", "lymphocyte", "nuclei", ",", "based", "on", "the", "use", "of", "carbobenzoxy-L-phenylalanine", "(", "CBZ-L-phe", ")", "to", "prevent", "breakdown", "of", "hormone-receptor", "complexes", "during", "extraction", "of", "nuclei", "with", "0.6M", "KCl", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Using", "this", "method", ",", "specific", "nuclear", "glucocorticoid", "binding", "was", "demonstrated", "in", "14/14", "patients", "with", "untreated", "CLL", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["No", "correlation", "was", "found", "between", "levels", "of", "nuclear-associated", "3H-TA", "and", "peripheral", "white", "blood", "cell", "count", "or", "rosetting", "ability", "of", "circulating", "lymphocytes", "."]} {"tags": [0, 0, 1, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Multiple", "closely-linked", "NFAT/octamer", "and", "HMG", "I", "(", "Y", ")", "binding", "sites", "are", "part", "of", "the", "interleukin-4", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["We", "show", "here", "that", "the", "immediate", "upstream", "region", "(", "from", "position", "-12", "to", "-270", ")", "of", "the", "murine", "interleukin", "4", "(", "Il-4", ")", "gene", "harbors", "a", "strong", "cell-type", "specific", "transcriptional", "enhancer", "."]} {"tags": [0, 5, 6, 6, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "T", "lymphoma", "cells", ",", "the", "activity", "of", "the", "Il-4", "promoter/enhancer", "is", "stimulated", "by", "phorbol", "esters", ",", "Ca++", "ionophores", "and", "agonists", "of", "protein", "kinase", "A", "and", "inhibited", "by", "low", "doses", "of", "the", "immunosuppressant", "cyclosporin", "A", "."]} {"tags": [0, 1, 2, 0, 0, 0, 0, 5, 6, 6, 0, 5, 6, 0], "tokens": ["The", "Il-4", "promoter/enhancer", "is", "transcriptionally", "inactive", "in", "B", "lymphoma", "cells", "and", "HeLa", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 0], "tokens": ["DNase", "I", "footprint", "protection", "experiments", "revealed", "six", "sites", "of", "the", "Il-4", "promoter/enhancer", "to", "be", "bound", "by", "nuclear", "proteins", "from", "lymphoid", "and", "myeloid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["Among", "them", "are", "four", "purine", "boxes", "which", "have", "been", "described", "to", "be", "important", "sequence", "motifs", "of", "the", "Il-2", "promoter", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0], "tokens": ["They", "contain", "the", "motif", "GGAAA", "and", "are", "recognized", "by", "the", "inducible", "and", "cyclosporin", "A-sensitive", "transcription", "factor", "NFAT-1", "."]} {"tags": [0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Three", "of", "the", "Il-4", "NFAT-1", "sites", "are", "closely", "linked", "to", "weak", "binding", "sites", "of", "Octamer", "factors", "."]} {"tags": [0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 3, 4, 4, 4, 0, 0], "tokens": ["Several", "purine", "boxes", "and", "an", "AT-rich", "protein-binding", "site", "of", "the", "Il-4", "promoter", "are", "also", "recognized", "by", "the", "high", "mobility", "group", "protein", "HMG", "I", "(", "Y", ")", "."]} {"tags": [0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 5, 6, 6, 0], "tokens": ["Whereas", "the", "binding", "of", "NFAT-1", "and", "Octamer", "factors", "enhance", "the", "activity", "of", "the", "Il-4", "promoter", ",", "the", "binding", "of", "HMG", "I", "(", "Y", ")", "suppresses", "its", "activity", "and", ",", "therefore", ",", "appears", "to", "be", "involved", "in", "the", "suppression", "of", "Il-4", "transcription", "in", "resting", "T", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Reversibility", "of", "the", "differentiated", "state", "in", "somatic", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["Analysis", "of", "de", "novo", "gene", "activation", "in", "multinucleated", "heterokaryons", "has", "shown", "that", "the", "differentiated", "state", ",", "although", "stable", ",", "is", "not", "irreversible", ",", "and", "can", "be", "reprogrammed", "in", "the", "presence", "of", "appropriate", "combinations", "of", "trans-acting", "regulatory", "molecules", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "properties", "have", "been", "exploited", "to", "design", "strategies", "for", "identifying", "novel", "regulators", "of", "cellular", "differentiation", "."]} {"tags": [0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 6, 0], "tokens": ["Phosphatidylcholine", "hydrolysis", "activates", "NF-kappa", "B", "and", "increases", "human", "immunodeficiency", "virus", "replication", "in", "human", "monocytes", "and", "T", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 3, 4, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0], "tokens": ["We", "have", "tested", "whether", "breakdown", "of", "phosphatidylcholine", "(", "PC", ")", "initiated", "by", "exogenous", "addition", "of", "a", "PC-specific", "phospholipase", "C", "(", "PC-PLC", ")", "from", "Bacillus", "cereus", "or", "by", "endogenous", "overexpression", "of", "PC-PLC", "induces", "functional", "activation", "of", "NF-kappa", "B", "and", "increases", "human", "immunodeficiency", "virus", "(", "HIV", ")", "enhancer", "activity", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0], "tokens": ["PC-PLC-activated", "hydrolysis", "of", "PC", "was", "found", "to", "induce", "bona", "fide", "p50/p65", "NF-kappa", "B", "binding", "activity", "in", "three", "different", "cell", "lines", "of", "human", "or", "murine", "origin", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0], "tokens": ["No", "significant", "changes", "in", "the", "turnover", "of", "other", "cellular", "phospholipids", "were", "detected", "in", "PC-PLC-treated", "cells", "."]} {"tags": [0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 3, 4, 0], "tokens": ["Induction", "of", "NF-kappa", "B", "by", "PC-PLC", "did", "not", "depend", "on", "de", "novo", "synthesis", "of", "proteins", "or", "autocrine", "secretion", "of", "either", "tumor", "necrosis", "factor", "or", "interleukin", "1", "."]} {"tags": [0, 7, 8, 8, 8, 8, 8, 0, 0, 0, 3, 4, 0, 3, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0], "tokens": ["In", "human", "monocytic", "and", "lymphoblastoid", "T-cell", "lines", ",", "induction", "of", "NF-kappa", "B", "by", "PC-PLC", "resulted", "in", "clear", "induction", "of", "luciferase", "expression", "vectors", "placed", "under", "the", "control", "of", "synthetic", "kappa", "B", "enhancers", "or", "wild", "type", ",", "but", "not", "kappa", "B-mutated", ",", "HIV", "long", "terminal", "repeat", "constructs", "."]} {"tags": [0, 0, 0, 0, 0, 3, 0, 0, 0, 5, 0, 0, 5, 0], "tokens": ["HIV", "replication", "was", "increased", "by", "PC-PLC", "in", "chronically", "infected", "monocytes", "and", "T", "lymphocytes", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["NF-kappa", "B", "activation", "promoted", "by", "addition", "of", "exogenous", "PC-PLC", "correlated", "with", "an", "intense", "production", "of", "diacylglycerol", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0], "tokens": ["However", ",", "addition", "of", "a", "phosphatidylinositol-specific", "PLC", "from", "B.cereus", "also", "induced", "diacylglycerol", "but", "did", "not", "activate", "kappa", "B", "enhancer-directed", "vectors", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0], "tokens": ["PC-PLC-induced", "NF-kappa", "B", "activation", "could", "not", "be", "blocked", "by", "a", "specific", "inhibitor", "of", "phorbol", "ester-inducible", "protein", "kinases", "C", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 5, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0], "tokens": ["These", "results", "indicate", "that", "a", "cellular", "transduction", "pathway", ",", "dependent", "on", "specific", "PC", "breakdown", ",", "is", "functional", "in", "T", "lymphocytes", "and", "monocytes", "and", "may", "be", "used", "by", "various", "transmembrane", "receptors", "to", "activate", "HIV", "transcription", "through", "NF-kappa", "B-dependent", "induction", "of", "the", "HIV", "enhancer", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0], "tokens": ["Novel", "mechanism", "for", "inhibition", "of", "human", "T", "cells", "by", "glucocorticoids", "."]} {"tags": [0, 0, 0, 0, 0, 3, 4, 0], "tokens": ["Glucocorticoids", "inhibit", "signal", "transduction", "through", "IL-2", "receptor", "."]} {"tags": [0, 0, 3, 0, 0, 3, 4, 4, 4, 4, 0, 3, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Interaction", "of", "IL-2", "with", "its", "high", "affinity", "membrane", "receptor", "complex", "(", "IL-2R", ")", "present", "on", "activated", "T", "lymphocytes", "induces", "cell", "proliferation", "and", "mediates", "effector", "functions", "."]} {"tags": [0, 0, 3, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Glucocorticoids", "inhibit", "IL-2", "production", "by", "inhibiting", "TCR-mediated", "signal", "transduction", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 3, 0], "tokens": ["We", "asked", "whether", "they", "also", "inhibit", "the", "action", "of", "IL-2", "by", "inhibiting", "signal", "transduction", "through", "IL-2R", "."]} {"tags": [5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Human", "peripheral", "blood", "T", "cells", ",", "stimulated", "with", "PMA", "for", "48", "h", "(", "PMA", "blasts", ")", ",", "were", "incubated", "with", "IL-2", "in", "the", "presence", "of", "incremental", "dosages", "of", "dexamethasone", "(", "Dex", ";", "10", "(", "-5", ")", "-10", "(", "-9", ")", "M", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Dex", "inhibited", "the", "IL-2-dependent", "proliferation", "of", "PMA", "blasts", "in", "a", "dose-dependent", "fashion", "(", "IC50", ",", "5", "x", "10", "(", "-8", ")", "M", ")", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Cell", "surface", "expression", "of", "IL-2R", "alpha-", "and", "beta-chains", "as", "determined", "by", "immunofluorescence", "analysis", "was", "not", "affected", "by", "Dex", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "addition", ",", "Scatchard", "plot", "analysis", "of", "125I-labeled", "IL-2", "showed", "that", "Dex", "did", "not", "affect", "the", "binding", "of", "IL-2", ",", "thus", "suggesting", "that", "inhibition", "is", "due", "to", "a", "postreceptor", "effect", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Inhibition", "of", "T", "cell", "proliferation", "by", "Dex", "was", "associated", "with", "decreased", "IL-2-dependent", "tyrosine", "phosphorylation", "of", "several", "intracellular", "proteins", "and", "decreased", "phosphorylation", "of", "the", "retinoblastoma", "gene", "product", "Rb", ",", "a", "protein", "essential", "for", "controlling", "the", "progression", "of", "cells", "through", "the", "cell", "cycle", "."]} {"tags": [0, 0, 0, 0, 0, 7, 8, 0, 3, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0], "tokens": ["IL-2-dependent", "IL-2R", "alpha", "expression", "in", "PMA", "blasts", "and", "NF-kB", "induction", "in", "resting", "human", "T", "cells", "were", "also", "inhibited", "by", "Dex", "."]} {"tags": [0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 3, 0], "tokens": ["These", "results", "demonstrate", "that", "glucocorticoids", "inhibit", "preactivated", "T", "cells", "by", "down-regulating", "signal", "transduction", "through", "IL-2R", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0], "tokens": ["Chronic", "human", "immunodeficiency", "virus", "type", "1", "infection", "stimulates", "distinct", "NF-kappa", "B/rel", "DNA", "binding", "activities", "in", "myelomonoblastic", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["The", "relationship", "between", "human", "immunodeficiency", "virus", "type", "1", "(", "HIV-1", ")", "infection", "and", "the", "induction", "of", "NF-kappa", "B", "binding", "activity", "was", "examined", "in", "a", "myeloid", "cell", "model", "of", "HIV-1", "infection", "derived", "from", "the", "PLB-985", "cell", "line", "."]} {"tags": [0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Chronic", "infection", "of", "PLB-985", "cells", "led", "to", "increased", "monocyte-specific", "surface", "marker", "expression", ",", "increased", "c-fms", "gene", "transcription", ",", "and", "morphological", "alterations", "consistent", "with", "differentiation", "along", "the", "monocytic", "pathway", "."]} {"tags": [7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0], "tokens": ["PLB-IIIB", "cells", "displayed", "a", "constitutive", "NF-kappa", "B-like", "binding", "activity", "that", "was", "distinct", "from", "that", "induced", "by", "tumor", "necrosis", "factor", "alpha", "or", "phorbol", "12-myristate", "13-acetate", "treatment", "of", "the", "parental", "PLB-985", "cell", "line", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 3, 4, 0, 3, 4, 0], "tokens": ["This", "unique", "DNA", "binding", "activity", "consisted", "of", "proteins", "of", "70", ",", "90", ",", "and", "100", "kDa", "with", "a", "high", "degree", "of", "binding", "specificity", "for", "the", "NF-kappa", "B", "site", "within", "the", "PRDII", "domain", "of", "beta", "interferon", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["In", "this", "report", ",", "we", "characterize", "the", "nature", "of", "these", "proteins", "and", "demonstrate", "that", "binding", "of", "these", "proteins", "is", "also", "induced", "following", "Sendai", "paramyxovirus", "infection", "."]} {"tags": [0, 3, 4, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["The", "70-kDa", "protein", "corresponds", "to", "the", "NF-kappa", "B", "RelA", "(", "p65", ")", "subunit", ",", "which", "is", "activated", "in", "response", "to", "an", "acute", "paramyxovirus", "infection", "or", "a", "chronic", "HIV-1", "infection", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 0, 3, 0, 3, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Virus", "infection", "does", "not", "appear", "to", "alter", "the", "amount", "of", "RelA", "(", "p65", ")", "or", "NFKB1", "(", "p50", ")", "but", "rather", "affects", "the", "capacity", "of", "I", "kappa", "B", "alpha", "to", "sequester", "RelA", "(", "p65", ")", ",", "therefore", "leading", "to", "constitutive", "levels", "of", "RelA", "DNA", "binding", "activity", "and", "to", "increased", "levels", "of", "NF-kappa", "B-dependent", "gene", "activity", "."]} {"tags": [0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 3, 0, 3, 0, 0, 3, 0, 3, 0, 0, 0, 3, 0], "tokens": ["The", "virally", "induced", "90-", "to", "100-kDa", "proteins", "have", "a", "distinct", "binding", "specificity", "for", "the", "PRDII", "domain", "and", "an", "AT-rich", "sequence", "but", "do", "not", "cross-react", "with", "NF-kappa", "B", "subunit-specific", "antisera", "directed", "against", "NFKB1", "(", "p105", "or", "p50", ")", ",", "NFKB2", "(", "p100", "or", "p52", ")", ",", "RelA", "(", "p65", ")", ",", "or", "c-rel", "."]} {"tags": [0, 0, 0, 0, 3, 4, 4, 4, 0, 0, 0, 0, 3, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0], "tokens": ["DNA", "binding", "of", "the", "90-", "to", "100-kDa", "proteins", "was", "not", "inhibited", "by", "recombinant", "I", "kappa", "B", "alpha/MAD-3", "and", "was", "resistant", "to", "tryptic", "digestion", ",", "suggesting", "that", "these", "proteins", "may", "not", "be", "NF-kappa", "B", "related", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0], "tokens": ["Transient", "cotransfection", "experiments", "demonstrated", "that", "RelA", "and", "NFKB1", "expression", "maximally", "stimulated", "HIV-1", "LTR-", "and", "NF-kappa", "B-dependent", "reporter", "genes", ";", "differences", "in", "NF-kappa", "B-like", "binding", "activity", "were", "also", "reflected", "in", "higher", "constitutive", "levels", "of", "NF-kappa", "B-regulated", "gene", "expression", "in", "HIV-1-infected", "myeloid", "cells", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 7, 8, 8, 8, 8, 8, 8, 0, 0, 0, 0, 0, 0], "tokens": ["Presence", "of", "estrogen-binding", "sites", "on", "macrophage-like", "synoviocytes", "and", "CD8+", ",", "CD29+", ",", "CD45RO+", "T", "lymphocytes", "in", "normal", "and", "rheumatoid", "synovium", "."]} {"tags": [0, 0], "tokens": ["OBJECTIVE", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "study", "the", "presence", "of", "estrogen-binding", "sites", "(", "EBS", ")", "in", "the", "synovial", "tissues", "of", "male", "and", "female", "patients", "with", "rheumatoid", "arthritis", "(", "RA", ")", "and", "in", "age-", "and", "sex-matched", "healthy", "controls", "."]} {"tags": [0, 0], "tokens": ["METHODS", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Both", "type", "1", "(", "high", "affinity", ",", "low", "binding", "capacity", ")", "and", "type", "2", "(", "reduced", "affinity", ",", "higher", "binding", "capacity", ")", "EBS", "were", "investigated", "in", "both", "soluble", "and", "nuclear", "fractions", "of", "homogenized", "synovial", "tissue", "samples", "by", "a", "dextran-coated", "charcoal", "method", "."]} {"tags": [0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["To", "determine", "what", "type", "of", "synovial", "cell", "was", "positive", "for", "EBS", ",", "cryosections", "of", "synovial", "tissues", "were", "immunostained", "with", "a", "specific", "monoclonal", "anti-estrogen", "receptor", "antibody", "(", "anti-ER", "MAb", ")", "using", "both", "immunofluorescence", "and", "immunoperoxidase", "techniques", "."]} {"tags": [0, 0, 0, 0, 3, 4, 0, 0, 3, 4, 0, 0, 0, 3, 4, 0, 3, 0, 3, 0, 3, 0, 0, 7, 8, 8, 8, 0, 7, 0, 7, 0, 7, 0, 7, 0, 0, 0, 0], "tokens": ["Double", "immunostaining", "with", "the", "anti-ER", "MAb", "and", "with", "specific", "MAb", "to", "detect", "different", "macrophage", "antigens", "(", "Ber-MAC3", ",", "MAC387", ",", "CD68", ")", "and", "CD8+", "T", "cell", "subsets", "(", "CD29+", ",", "CD45RO+", "and", "CD29-", ",", "CD45RO-", ")", "was", "performed", "."]} {"tags": [0, 0], "tokens": ["RESULTS", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Higher", "affinity", "EBS", "were", "found", "mostly", "in", "nuclear", "cell", "fractions", "of", "either", "RA", "or", "control", "synovial", "tissues", "(", "28", "of", "the", "33", ")", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["These", "EBS", "were", "present", "to", "a", "lesser", "extent", "in", "soluble", "cell", "fractions", "(", "11", "of", "the", "33", ")", "."]} {"tags": [0, 0, 0, 5, 6, 6, 0, 0, 0, 5, 6, 0, 0, 7, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0], "tokens": ["Immunostaining", "showed", "the", "estrogen", "receptor-positive", "cells", "to", "be", "the", "macrophage-like", "synoviocytes", "and", "the", "CD8+", ",", "CD29+", "T", "cells", "both", "in", "RA", "and", "in", "control", "synovial", "tissues", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 5, 6, 0], "tokens": ["Higher", "nuclear", "content", "of", "EBS", "was", "consistent", "with", "more", "intense", "nuclear", "staining", "of", "synoviocytes", "and", "T", "cells", "."]} {"tags": [0, 0], "tokens": ["CONCLUSION", "."]} {"tags": [0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 0, 7, 0, 7], "tokens": ["It", "is", "conceivable", "that", "the", "immunomodulatory", "activity", "exerted", "by", "estrogens", "is", "at", "least", "partly", "mediated", "through", "their", "interaction", "with", "EBS", "that", "are", "present", "on", "macrophage-like", "synoviocytes", ",", "functioning", "as", "antigen-processing", "and", "antigen-presenting", "cells", ",", "and", "on", "antigen-experienced", "(", "memory", ")", "CD8+", "T", "lymphocytes", "(", "CD29+", ",", "CD45RO+"]}