text: The sensitivity of DAT for IMHA in dogs is widely debated ranging from 50% to 89%.(6,8,11,12,18,22,29,30) False-negative DAT results have been attributed to technical difficulties with the assay, such as inappropriate or inadequate strength of antisera, exclusive cold agglutinins, failing to adequately wash RBCs, prozone effect, low affinity or quantities of autoantibodies on RBCs, or presence of drug-induced autoantibodies.