text: For experiments studying FMT (44), fibroblasts were cultured in DMEM/F12 10% FBS at low density for 24 hours, then at 40%–50% confluency, media was changed to DMEM/F12 1% FBS for 4 hours prior to treatments with SMOC2 (Preprotech) and/or TGFβ1(Preprotech).