IEPA.d91.s215
Oxytocin-stimulated insulin release in a clonal beta-cell line RINm5F: involvement of phospholipase C-dependent and -independent pathways.
IEPA.d91.s216
CONCLUSION: Oxy increases insulin release through both PLC and non-PLC mediated signal transduction mechanisms.
IEPA.d91.s217
In addition, U-73122 diminished the Oxy-induced increase in intracellular concentration of inositol 1, 4, 5-triphosphate (IP3)
IEPA.d91.s218
U-73122 at 8 mumol.L-1 totally abolished the Oxy-induced increases in [Ca2+]i and IP3; however it reduced the Oxy-induced increase in insulin release only by 36% and 63% in the monolayer and suspended cell preparations, respectively
IEPA.d91.s219
Oxytocin-stimulated insulin release in a clonal beta-cell line RINm5F: involvement of phospholipase C-dependent and -independent pathways
IEPA.d91.s220
AIM: To study the mechanisms underlying oxytocin (Oxy)-induced insulin release
IEPA.d91.s221
RESULTS: Oxy increased insulin release and [Ca2+]i in a concentration-dependent manner
IEPA.d91.s222
Oxy-induced insulin release was not altered by pretreatment with pertussis toxin (PT)
IEPA.d91.s223
U-73122 at 8 mumol.L-1 totally abolished the Oxy-induced increases in [Ca2+]i and IP3; however it reduced the Oxy-induced increase in insulin release only by 36% and 63% in the monolayer and suspended cell preparations, respectively
IEPA.d91.s224
CONCLUSION: Oxy increases insulin release through both PLC and non-PLC mediated signal transduction mechanisms