IEPA.d17.s34
The aim of this study was to investigate the effects of hCG, hCG plus oxytocin and oxytocin on [3H] inositol phosphate (IP) formations in porcine myometrial cells obtained from ovariectomized and cyclic gilts
IEPA.d17.s35
The highest production of total inositol phosphates (d.p.m.) was found in cells from ovariectomized gilts given in vivo estradiol benzoate and progesterone for five consecutive days and treated with 1000 mU hCG and 100 mU hCG plus 1 microM oxytocin (984 +/- 84 and 1063 +/- 131 vs 314 +/- 36, respectively)
IEPA.d17.s36
There was also a very significant increase of IP1 after the addition of 1000 mU hCG (p < 0.001) and IP1 and IP3 when 1000 mU hCG plus oxytocin were added (p < 0.001 and p < 0.01, respectively)
IEPA.d17.s37
Only oxytocin alone increased the formation of IPs in cells from ovariectomized pigs treated with estradiol in vivo (p < 0.01)
IEPA.d17.s38
The highest dose of hCG plus The highest dose of hCG plus oxytocin provoked accumulation of total [3H]inositol phosphate (p < 0.05) 53% over the basal level on days 21/1 of the estrous cycle
IEPA.d17.s39
The present study demonstrates that hCG and oxytocin can increase the accumulation of inositol phosphates in porcine myometrial cells