IEPA.d194.s465
Insulin-induced phospholipase D1 and phospholipase D2 activity in human embryonic kidney-293 cells mediated by the phospholipase Cgamma and protein kinase Calpha signalling cascade.
IEPA.d194.s466
Co-expression of PLD1 with phospholipase C (PLC)gamma has the same effect, while co-expression of PLD2 with PLCgamma allows PLD2 activity to be stimulated in an insulin-dependent manner.
IEPA.d194.s467
The PKC-specific inhibitors bisindolylmaleimide and Go 6976 abolish insulin-induced PLD2 activation in HEK-293 cells co-expressing the insulin receptor, PLCgamma and PLD2, confirming that not only PLD1, but PLD2 as well, is regulated in a PKC-dependent manner.
IEPA.d194.s468
In summary, we demonstrate that insulin treatment results in activation of both PLD1 and PLD2 in appropriate cell types when the appropriate upstream intermediate signalling components, i.e. PKCalpha and PLCgamma, are expressed at sufficient levels.