sentences.id: IEPA.d138.s327
sentences.origID: 32
sentences.text: Insulin-like signaling in yeast: modulation of protein phosphatase 2A, protein kinase A, cAMP-specific phosphodiesterase, and glycosyl-phosphatidylinositol-specific phospholipase C activities
sentences.id: IEPA.d138.s327.e0
sentences.origID: 76
sentences.text: Insulin
sentences.id: IEPA.d138.s327.e1
sentences.origID: 77
sentences.text: phospholipase C
sentences.id: IEPA.d138.s328
sentences.origID: 33
sentences.text: In addition, glycosyl-phosphatidylinositol-specific phospholipase C (GPI-PLC), which in isolated rat adipocytes is activated by insulin, was stimulated to up to 5-fold by glucose and 10-fold by glucose plus insulin in both yeast spheroplasts and intact cells leading to a concentration-dependent leftward shift of the glucose-response curve for activation of the GPI-PLC
sentences.id: IEPA.d138.s328.e0
sentences.origID: 78
sentences.text: glycosyl-phosphatidylinositol-specific phospholipase C
sentences.id: IEPA.d138.s328.e1
sentences.origID: 79
sentences.text: insulin
sentences.id: IEPA.d138.s328.e2
sentences.origID: 80
sentences.text: insulin
sentences.id: IEPA.d138.s328.e3
sentences.origID: 81
sentences.text: GPI-PLC
sentences.id: IEPA.d138.s328.i0
sentences.e1: IEPA.d138.s328.e0
sentences.e2: IEPA.d138.s328.e1
sentences.type: PPI
sentences.id: IEPA.d138.s328.i1
sentences.e1: IEPA.d138.s328.e0
sentences.e2: IEPA.d138.s328.e2
sentences.type: PPI
sentences.id: IEPA.d138.s329
sentences.origID: 34
sentences.text: GPI-PLC was most pronouncedly stimulated by authentic human insulin compared to various insulin analogues and insulin-like growth factor I
sentences.id: IEPA.d138.s329.e0
sentences.origID: 82
sentences.text: GPI-PLC
sentences.id: IEPA.d138.s329.e1
sentences.origID: 83
sentences.text: insulin
sentences.id: IEPA.d138.s329.i0
sentences.e1: IEPA.d138.s329.e0
sentences.e2: IEPA.d138.s329.e1
sentences.type: PPI
sentences.id: IEPA.d138.s330
sentences.origID: 35
sentences.text: As the GPI-PLC reaction is rate limiting, the efficiency of the two-step anchor cleavage was significantly increased when insulin was present together with glucose as compared to glucose alone
sentences.id: IEPA.d138.s330.e0
sentences.origID: 84
sentences.text: GPI-PLC
sentences.id: IEPA.d138.s330.e1
sentences.origID: 85
sentences.text: insulin
sentences.id: IEPA.d138.s331
sentences.origID: 36
sentences.text: The insulin concentrations effective in modulating PP2A, PKA, cAMP-PDE, and GPI-PLC activities correlate well with those required for half-saturation of the specific binding sites as well as for stimulation of protein phosphorylation and glycogen accumulation
sentences.id: IEPA.d138.s331.e0
sentences.origID: 86
sentences.text: insulin
sentences.id: IEPA.d138.s331.e1
sentences.origID: 87
sentences.text: GPI-PLC
sentences.id: IEPA.d138.s331.i0
sentences.e1: IEPA.d138.s331.e0
sentences.e2: IEPA.d138.s331.e1
sentences.type: PPI
sentences.id: IEPA.d138.s332
sentences.origID: 37
sentences.text: The data suggest that mammalian insulin-sensitive cells and yeast share (part of) the key regulatory mechanism (consisting of PP2A, PKA, cAMP-PDE, and GPI-PLC) involved in the transduction of the insulin signal from the respective receptor systems to glycogen synthase and phosphorylase
sentences.id: IEPA.d138.s332.e0
sentences.origID: 88
sentences.text: insulin
sentences.id: IEPA.d138.s332.e1
sentences.origID: 89
sentences.text: GPI-PLC
sentences.id: IEPA.d138.s332.e2
sentences.origID: 90
sentences.text: insulin
sentences.id: IEPA.d138.s332.i0
sentences.e1: IEPA.d138.s332.e1
sentences.e2: IEPA.d138.s332.e2
sentences.type: PPI