sentences.id: IEPA.d194.s465
sentences.origID: 15
sentences.text: Insulin-induced phospholipase D1 and phospholipase D2 activity in human embryonic kidney-293 cells mediated by the phospholipase Cgamma and protein kinase Calpha signalling cascade.
sentences.id: IEPA.d194.s465.e0
sentences.origID: 37
sentences.text: Insulin
sentences.id: IEPA.d194.s465.e1
sentences.origID: 38
sentences.text: phospholipase Cgamma
sentences.id: IEPA.d194.s465.i0
sentences.e1: IEPA.d194.s465.e0
sentences.e2: IEPA.d194.s465.e1
sentences.type: PPI
sentences.id: IEPA.d194.s466
sentences.origID: 16
sentences.text: Co-expression of PLD1 with phospholipase C (PLC)gamma has the same effect, while co-expression of PLD2 with PLCgamma allows PLD2 activity to be stimulated in an insulin-dependent manner.
sentences.id: IEPA.d194.s466.e0
sentences.origID: 39
sentences.text: phospholipase C (PLC)gamma
sentences.id: IEPA.d194.s466.e1
sentences.origID: 40
sentences.text: PLCgamma
sentences.id: IEPA.d194.s466.e2
sentences.origID: 41
sentences.text: insulin
sentences.id: IEPA.d194.s467
sentences.origID: 17
sentences.text: The PKC-specific inhibitors bisindolylmaleimide and Go 6976 abolish insulin-induced PLD2 activation in HEK-293 cells co-expressing the insulin receptor, PLCgamma and PLD2, confirming that not only PLD1, but PLD2 as well, is regulated in a PKC-dependent manner.
sentences.id: IEPA.d194.s467.e0
sentences.origID: 42
sentences.text: insulin
sentences.id: IEPA.d194.s467.e1
sentences.origID: 43
sentences.text: PLCgamma
sentences.id: IEPA.d194.s468
sentences.origID: 18
sentences.text: In summary, we demonstrate that insulin treatment results in activation of both PLD1 and PLD2 in appropriate cell types when the appropriate upstream intermediate signalling components, i.e. PKCalpha and PLCgamma, are expressed at sufficient levels.
sentences.id: IEPA.d194.s468.e0
sentences.origID: 44
sentences.text: insulin
sentences.id: IEPA.d194.s468.e1
sentences.origID: 45
sentences.text: PLCgamma
sentences.id: IEPA.d194.s468.i0
sentences.e1: IEPA.d194.s468.e0
sentences.e2: IEPA.d194.s468.e1
sentences.type: PPI