sentences.id: IEPA.d146.s351
sentences.origID: 211
sentences.text: Ca2+ signalling in rat vascular smooth muscle cells: a role for protein kinase C at physiological vasoconstrictor concentrations of vasopressin
sentences.id: IEPA.d146.s351.e0
sentences.origID: 458
sentences.text: protein kinase C
sentences.id: IEPA.d146.s351.e1
sentences.origID: 459
sentences.text: vasopressin
sentences.id: IEPA.d146.s351.i0
sentences.e1: IEPA.d146.s351.e0
sentences.e2: IEPA.d146.s351.e1
sentences.type: PPI
sentences.id: IEPA.d146.s352
sentences.origID: 212
sentences.text: The protein kinase C (PKC) activator 4beta-phorbol 12-myristate 13-acetate (PMA, 100 pM to 200 nM), also stimulated Ca2+ spiking and this effect was additive with a submaximal concentration of AVP (50 pM)
sentences.id: IEPA.d146.s352.e0
sentences.origID: 460
sentences.text: protein kinase C
sentences.id: IEPA.d146.s352.e1
sentences.origID: 461
sentences.text: AVP
sentences.id: IEPA.d146.s353
sentences.origID: 213
sentences.text: The PKC inhibitors Ro-31-8220 (1 microM) and calphostin C (250 nM) completely blocked the stimulation of Ca2+ spiking by either PMA or AVP
sentences.id: IEPA.d146.s353.e0
sentences.origID: 462
sentences.text: PKC inhibitors
sentences.id: IEPA.d146.s353.e1
sentences.origID: 463
sentences.text: AVP
sentences.id: IEPA.d146.s353.i0
sentences.e1: IEPA.d146.s353.e0
sentences.e2: IEPA.d146.s353.e1
sentences.type: PPI
sentences.id: IEPA.d146.s354
sentences.origID: 214
sentences.text: Time-dependent redistribution of PKC-alpha, -delta and -epsilon isoforms between the membrane and cytosolic fractions occurred in response to 100 pM AVP
sentences.id: IEPA.d146.s354.e0
sentences.origID: 464
sentences.text: PKC-alpha
sentences.id: IEPA.d146.s354.e1
sentences.origID: 465
sentences.text: AVP
sentences.id: IEPA.d146.s354.i0
sentences.e1: IEPA.d146.s354.e0
sentences.e2: IEPA.d146.s354.e1
sentences.type: PPI
sentences.id: IEPA.d146.s355
sentences.origID: 215
sentences.text: Pretreatment for 24 h with 1 microM PMA downregulated expression of PKC-alpha and -delta, but not PKC-epsilon, and prevented the Ca2+-spiking responses to either 1 nM PMA or 100 pM AVP
sentences.id: IEPA.d146.s355.e0
sentences.origID: 466
sentences.text: PKC-alpha
sentences.id: IEPA.d146.s355.e1
sentences.origID: 467
sentences.text: PKC-epsilon
sentences.id: IEPA.d146.s355.e2
sentences.origID: 468
sentences.text: AVP
sentences.id: IEPA.d146.s356
sentences.origID: 216
sentences.text: We conclude that PKC activation is a necessary step in the signal transduction pathway linking low concentrations of AVP to Ca2+ spiking in A7r5 cells
sentences.id: IEPA.d146.s356.e0
sentences.origID: 469
sentences.text: PKC
sentences.id: IEPA.d146.s356.e1
sentences.origID: 470
sentences.text: AVP
sentences.id: IEPA.d146.s356.i0
sentences.e1: IEPA.d146.s356.e0
sentences.e2: IEPA.d146.s356.e1
sentences.type: PPI