IEPA.d115.s277
40
Overexpression of G11alpha and isoforms of phospholipase C in islet  beta-cells reveals a lack of correlation between inositol phosphate  accumulation and insulin secretion.
IEPA.d115.s277.e0
95
phospholipase C
IEPA.d115.s277.e1
96
insulin
IEPA.d115.s278
41
It has been suggested that insulin secretion from pancreatic islets may be  mediated in part by activation of phospholipases C (PLCs) and  phosphoinositide hydrolysis.
IEPA.d115.s278.e0
97
insulin
IEPA.d115.s278.e1
98
phospholipases C
IEPA.d115.s278.i0
IEPA.d115.s278.e0
IEPA.d115.s278.e1
PPI
IEPA.d115.s279
42
The purpose of this study was to determine  whether the relatively modest fuel-stimulated insulin secretion responses  of rodent beta-cell lines might be explained by inadequate expression or  activation of PLC isoforms.
IEPA.d115.s279.e0
99
insulin
IEPA.d115.s279.e1
100
PLC
IEPA.d115.s279.i0
IEPA.d115.s279.e0
IEPA.d115.s279.e1
PPI
IEPA.d115.s280
43
Adenovirus-mediated overexpression of PLC-delta1, -beta1, or  -beta3 in INS-1 or betaG 40/110 cells results in little or no enhancement  in inositol phosphate (IP) accumulation and no improvement in insulin  secretion when the cells are stimulated with glucose or carbachol, despite  the fact that the overexpressed proteins are fully active in cell  extracts.
IEPA.d115.s280.e0
101
PLC
IEPA.d115.s280.e1
102
insulin
IEPA.d115.s281
44
Overexpression of PLC-beta1 or -beta3 in normal rat islets  elicits a larger increase in IP accumulation but, again, has no effect on  insulin secretion.
IEPA.d115.s281.e0
103
PLC
IEPA.d115.s281.e1
104
insulin
IEPA.d115.s282
45
Because the effect of carbachol on insulin secretion is  thought to be mediated through muscarinic receptors that link to the Gq/11  class of heterotrimeric G proteins, we also overexpressed G11alpha in  INS-1 cells, either alone or in concert with overexpression of PLC-beta1  or -beta3.
IEPA.d115.s282.e0
105
insulin
IEPA.d115.s282.e1
106
PLC
IEPA.d115.s283
46
Overexpression of G11alpha enhances IP accumulation, an effect  slightly potentiated by co-overexpression of PLC-beta1 or -beta3, but  these maneuvers do not affect glucose or carbachol-stimulated insulin  secretion.
IEPA.d115.s283.e0
107
PLC
IEPA.d115.s283.e1
108
insulin