IEPA.d50.s127
68
Insulin secretion is regulated by changes in blood glucose, as well as by peptides from the gastrointestinal tract and neurotransmitters that activate the pancreatic islet adenylyl cyclase (e.g., glucagon-like peptide-1) and phospholipase C (PLC) (e.g., acetylcholine) signaling pathways to further potentiate glucose-induced insulin secretion
IEPA.d50.s127.e0
153
Insulin
IEPA.d50.s127.e1
154
phospholipase C
IEPA.d50.s127.e2
155
insulin
IEPA.d50.s127.i0
IEPA.d50.s127.e1
IEPA.d50.s127.e2
PPI
IEPA.d50.s128
69
However, leptin did constrain the enhanced PLC-mediated insulin secretion characteristic of islets from ob/ob mice, without influencing release from islets of lean mice
IEPA.d50.s128.e0
156
PLC
IEPA.d50.s128.e1
157
insulin
IEPA.d50.s128.i0
IEPA.d50.s128.e0
IEPA.d50.s128.e1
PPI
IEPA.d50.s129
70
A specific enhancement in PLC-mediated insulin secretion is the earliest reported developmental alteration in insulin secretion from islets of ob/ob mice, and thus a logical target for leptin action
IEPA.d50.s129.e0
158
PLC
IEPA.d50.s129.e1
159
insulin
IEPA.d50.s129.e2
160
insulin
IEPA.d50.s129.i0
IEPA.d50.s129.e0
IEPA.d50.s129.e1
PPI
IEPA.d50.s130
71
This action of leptin on PLC-mediated insulin secretion was dose-dependent, rapid-onset (i.e., within 3 min), and reversible
IEPA.d50.s130.e0
161
PLC
IEPA.d50.s130.e1
162
insulin
IEPA.d50.s130.i0
IEPA.d50.s130.e0
IEPA.d50.s130.e1
PPI
IEPA.d50.s131
72
Leptin was equally effective in constraining the enhanced insulin release from islets of ob/ob mice caused by protein kinase C (PKC) activation, a downstream mediator of the PLC signal pathway
IEPA.d50.s131.e0
163
insulin
IEPA.d50.s131.e1
164
PLC
IEPA.d50.s132
73
One function of leptin in control of body composition is thus to target a PKC-regulated component of the PLC-PKC signaling system within islets to prevent hypersecretion of insulin
IEPA.d50.s132.e0
165
PLC
IEPA.d50.s132.e1
166
insulin