A cDNA encoding the complete amino acid sequence of aminoacylase 1 (N-acylamino acid aminohydrolase, ACY-1) [<< EC 3.5.1.14 >>], a dimeric metalloprotein having two Zn2+ in the molecule, which catalyzes the deacylation of [[ N-acylated L-amino acids ]] except L-aspartic acid, has been isolated from porcine kidney lambda gt10 cDNA library and sequenced.